AlfalfaGEDB | Msa1332450

M. sativa cultivar zhongmu-4 / Msa1332450

KEGG: K03955
GO: GO:0000035 GO:0000036 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009245 GO:0009247 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016053 GO:0019637 GO:0019752 GO:0019842 GO:0031177 GO:0032787 GO:0033218 GO:0036094 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0044620 GO:0046394 GO:0046467 GO:0046493 GO:0048037 GO:0051192 GO:0071704 GO:0072330 GO:0072341 GO:0090407 GO:0140104 GO:1901135 GO:1901137 GO:1901269 GO:1901271 GO:1901576 GO:1903509
PFAM: PF00550.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332450	PNY14930.1	95.161	124	6	0	1	124	1	124	1.61e-79	239

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332450	sp\|Q9FGJ4\|ACPM3_ARATH	56.693	127	51	1	1	123	1	127	1.07e-41	137

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332450	A0A2K3PI09	95.161	124	6	0	1	124	1	124	7.68e-80	239

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008330	Msa1332450	0.801541	8.577067e-49	-8.615850e-47
Msa0036150	Msa1332450	0.821571	3.762120e-53	-8.615850e-47
Msa0040190	Msa1332450	0.813525	2.449761e-51	-8.615850e-47
Msa0075850	Msa1332450	0.804871	1.754029e-49	-8.615850e-47
Msa0619330	Msa1332450	0.809552	1.788740e-50	-8.615850e-47
Msa0739570	Msa1332450	0.822111	2.820650e-53	-8.615850e-47
Msa0749550	Msa1332450	0.803043	4.208962e-49	-8.615850e-47
Msa0756480	Msa1332450	0.814825	1.264597e-51	-8.615850e-47
Msa0782330	Msa1332450	0.805713	1.168851e-49	-8.615850e-47
Msa0792510	Msa1332450	0.813692	2.250477e-51	-8.615850e-47
Msa0835510	Msa1332450	0.817957	2.518459e-52	-8.615850e-47
Msa0134550	Msa1332450	0.812715	3.687639e-51	-8.615850e-47
Msa0168390	Msa1332450	0.800750	1.244830e-48	-8.615850e-47
Msa0189050	Msa1332450	0.822076	2.874157e-53	-8.615850e-47
Msa0231310	Msa1332450	0.822485	2.310177e-53	-8.615850e-47
Msa1254770	Msa1332450	0.805362	1.384651e-49	-8.615850e-47
Msa1254800	Msa1332450	0.804267	2.344767e-49	-8.615850e-47
Msa1332450	Msa1337190	0.947346	8.044868e-106	-8.615850e-47
Msa1332450	Msa1355260	0.833443	5.316716e-56	-8.615850e-47
Msa1332450	Msa1373950	0.816862	4.443595e-52	-8.615850e-47
Msa1332450	Msa1387090	0.806091	9.734659e-50	-8.615850e-47
Msa1332450	Msa1389120	0.993105	1.743915e-197	-8.615850e-47
Msa1332450	Msa1390410	0.826008	3.433270e-54	-8.615850e-47
Msa1332450	Msa1409520	0.800946	1.135442e-48	-8.615850e-47
Msa0365360	Msa1332450	0.821127	4.762279e-53	-8.615850e-47
Msa0371610	Msa1332450	0.808692	2.733443e-50	-8.615850e-47
Msa0413740	Msa1332450	0.803350	3.634990e-49	-8.615850e-47
Msa0418000	Msa1332450	0.824722	6.918799e-54	-8.615850e-47
Msa0431710	Msa1332450	0.800721	1.261665e-48	-8.615850e-47
Msa0466100	Msa1332450	0.803572	3.270626e-49	-8.615850e-47
Msa0475830	Msa1332450	0.819218	1.303698e-52	-8.615850e-47
Msa1009000	Msa1332450	0.800610	1.329289e-48	-8.615850e-47
Msa1064420	Msa1332450	0.803706	3.067789e-49	-8.615850e-47
Msa1081600	Msa1332450	0.846158	2.589011e-59	-8.615850e-47
Msa0257930	Msa1332450	-0.801387	9.222995e-49	-8.615850e-47
Msa0286790	Msa1332450	0.808280	3.346436e-50	-8.615850e-47
Msa0304990	Msa1332450	0.815042	1.132051e-51	-8.615850e-47
Msa0306130	Msa1332450	0.830792	2.404847e-55	-8.615850e-47
Msa0309160	Msa1332450	0.824120	9.589573e-54	-8.615850e-47
Msa1166370	Msa1332450	0.832756	7.882381e-56	-8.615850e-47
Msa1208070	Msa1332450	0.800130	1.664922e-48	-8.615850e-47
Msa1220710	Msa1332450	0.804276	2.334319e-49	-8.615850e-47
Msa1224400	Msa1332450	0.986913	2.098991e-168	-8.615850e-47
Msa0498920	Msa1332450	0.804853	1.769493e-49	-8.615850e-47
Msa0504630	Msa1332450	0.806668	7.356257e-50	-8.615850e-47
Msa0535020	Msa1332450	0.809846	1.546420e-50	-8.615850e-47
Msa0535050	Msa1332450	0.816069	6.687682e-52	-8.615850e-47
Msa0591780	Msa1332450	0.814416	1.557873e-51	-8.615850e-47
Msa0855520	Msa1332450	0.813304	2.738674e-51	-8.615850e-47
Msa0860270	Msa1332450	0.836808	7.530795e-57	-8.615850e-47
Msa0960270	Msa1332450	0.806091	9.734335e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332450	MtrunA17_Chr4g0076301	94.355	124	7	1	124	1	124	6.58e-83	238
Msa1332450	MtrunA17_Chr7g0249561	50.649	77	38	47	123	54	130	2.02e-21	82.8
Msa1332450	MtrunA17_Chr7g0249801	48.649	74	38	47	120	43	116	2.03e-21	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332450	AT5G47630.1	56.693	127	51	1	1	123	1	127	1.09e-42	137
Msa1332450	AT5G47630.2	56.693	127	51	1	1	123	1	127	1.09e-42	137
Msa1332450	AT5G47630.3	56.693	127	51	1	1	123	1	127	1.09e-42	137
Msa1332450	AT1G65290.1	50.649	77	38	0	47	123	50	126	3.52e-21	82.4
Msa1332450	AT2G44620.1	41.000	100	46	1	17	116	29	115	6.34e-21	81.6

Find 34 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCAACAAAACTTCTCTTTA+TGG	0.154842	8_4:-52410710	Msa1332460:three_prime_UTR
GGATAGGGTTGAGCTTATTA+TGG	0.307345	8_4:+52410531	Msa1332450:CDS
AGTAAAAGTGAAAATAATAT+TGG	0.326289	8_4:-52410074	None:intergenic
AGCTTATCTGCTTTCTCATC+AGG	0.361227	8_4:-52410583	Msa1332460:three_prime_UTR
GCAAAACAATTGAAAAGTTA+TGG	0.376214	8_4:-52410669	Msa1332460:three_prime_UTR
ATATGCCGACAAAATTTGAT+TGG	0.384777	8_4:-52409166	None:intergenic
CACTCACTGGGAGATTCGTT+TGG	0.407167	8_4:-52409189	None:intergenic
TCAGGTGTCATGCTTACATT+GGG	0.419498	8_4:-52410821	Msa1332460:three_prime_UTR
ATCAGGTGTCATGCTTACAT+TGG	0.439491	8_4:-52410822	Msa1332460:three_prime_UTR
CATAATCACATTATCATCCT+TGG	0.439969	8_4:-52410237	Msa1332460:three_prime_UTR
GGACTTAAACCTGGACAGCT+TGG	0.459469	8_4:+52410510	Msa1332450:CDS
TGATTATGCATTTGAGATGT+TGG	0.460487	8_4:+52410252	Msa1332450:CDS
TGAGAAGGTCAACTGAAAGA+TGG	0.475010	8_4:+52410207	Msa1332450:CDS
GTCTAGTATCGGATCAGAGC+TGG	0.475971	8_4:-52410291	Msa1332460:three_prime_UTR
TGTCATGCTTACATTGGGGT+GGG	0.479726	8_4:-52410816	Msa1332460:three_prime_UTR
AGATGTTGGTGCTCTTCAGC+AGG	0.487417	8_4:+52410266	Msa1332450:CDS
CAACCCTATCCAAGCTGTCC+AGG	0.498453	8_4:-52410519	Msa1332460:three_prime_UTR
TGCCACTTTGAATTAGCACT+TGG	0.508091	8_4:+52410122	Msa1332450:five_prime_UTR
TTCAATAAAGCATAACTCAA+CGG	0.513754	8_4:-52410892	Msa1332460:three_prime_UTR
TAAACCTGGACAGCTTGGAT+AGG	0.517739	8_4:+52410515	Msa1332450:CDS
GTGTCATGCTTACATTGGGG+TGG	0.521344	8_4:-52410817	Msa1332460:three_prime_UTR
TCTTGACCAGTCTGATTACT+CGG	0.521850	8_4:-52410313	Msa1332460:three_prime_UTR
AAACCTGGACAGCTTGGATA+GGG	0.522123	8_4:+52410516	Msa1332450:CDS
CTGATTACTCGGTCTAGTAT+CGG	0.536163	8_4:-52410302	Msa1332460:three_prime_UTR
GTCATGCTTACATTGGGGTG+GGG	0.536900	8_4:-52410815	Msa1332460:three_prime_UTR
GGCTATGTACTTTGCAACAT+CGG	0.551029	8_4:-52410617	Msa1332460:three_prime_UTR
TTTCTCATCAGGAATTTCGA+TGG	0.580152	8_4:-52410572	Msa1332460:three_prime_UTR
TGATAAAATTAATGCAGCAA+AGG	0.580568	8_4:+52410340	Msa1332450:CDS
TGTACGAAAATAGAAAAGCA+AGG	0.585420	8_4:-52410855	Msa1332460:three_prime_UTR
GTACGAAAATAGAAAAGCAA+GGG	0.606712	8_4:-52410854	Msa1332460:three_prime_UTR
TAAGTCATGTGAATTTGAGA+AGG	0.613186	8_4:+52410192	Msa1332450:CDS
ATCCAAGTGCTAATTCAAAG+TGG	0.625412	8_4:-52410124	None:intergenic
ACTAGACCGAGTAATCAGAC+TGG	0.680991	8_4:+52410307	Msa1332450:CDS
CAGGTGTCATGCTTACATTG+GGG	0.762397	8_4:-52410820	Msa1332460:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGTCATTTTTTATTTTTTTT+TGG	+	chr8_4:52409528-52409547	Msa1332450:intron	10.0%
!!!	GTCATTTTTTATTTTTTTTT+GGG	+	chr8_4:52409529-52409548	Msa1332450:intron	10.0%
!!!	TCATTTTTTATTTTTTTTTG+GGG	+	chr8_4:52409530-52409549	Msa1332450:intron	10.0%
!!!	TGTCATTTTTTATTTTTTTT+TGG	+	chr8_4:52409528-52409547	Msa1332450:intron	10.0%
!!!	GTCATTTTTTATTTTTTTTT+GGG	+	chr8_4:52409529-52409548	Msa1332450:intron	10.0%
!!!	TCATTTTTTATTTTTTTTTG+GGG	+	chr8_4:52409530-52409549	Msa1332450:intron	10.0%
!!!	CATTTTTTATTTTTTTTTGG+GGG	+	chr8_4:52409531-52409550	Msa1332450:intron	15.0%
!!!	GATTTTGTTATTTTTAATGA+AGG	+	chr8_4:52409938-52409957	Msa1332450:intron	15.0%
!!	AGTAAAAGTGAAAATAATAT+TGG	-	chr8_4:52410077-52410096	None:intergenic	15.0%
!!	TGACATAAAAAAATAAGAAA+AGG	-	chr8_4:52410795-52410814	Msa1332460:three_prime_UTR	15.0%
!!!	CATTTTTTATTTTTTTTTGG+GGG	+	chr8_4:52409531-52409550	Msa1332450:intron	15.0%
!!!	GATTTTGTTATTTTTAATGA+AGG	+	chr8_4:52409938-52409957	Msa1332450:intron	15.0%
!!	AGTAAAAGTGAAAATAATAT+TGG	-	chr8_4:52410077-52410096	None:intergenic	15.0%
!!	TGACATAAAAAAATAAGAAA+AGG	-	chr8_4:52410795-52410814	Msa1332460:three_prime_UTR	15.0%
!!!	TTAATCCAATCAAATTTTGT+CGG	+	chr8_4:52409161-52409180	Msa1332450:five_prime_UTR	20.0%
!!	AATTCAACAATAGAAGTAAA+TGG	+	chr8_4:52409592-52409611	Msa1332450:intron	20.0%
!!	AAATAAAAAGTAGAAGTCAT+AGG	-	chr8_4:52409889-52409908	None:intergenic	20.0%
!!	TGCATGTTTATTTATATGAA+TGG	+	chr8_4:52409908-52409927	Msa1332450:intron	20.0%
!!!	TATTTTCTTTTGACTATAAC+TGG	-	chr8_4:52410370-52410389	Msa1332460:three_prime_UTR	20.0%
!!!	ATTGGTATATATTTTTGTGT+AGG	+	chr8_4:52410462-52410481	Msa1332450:intron	20.0%
!!!	TTAATCCAATCAAATTTTGT+CGG	+	chr8_4:52409161-52409180	Msa1332450:five_prime_UTR	20.0%
!!	AATTCAACAATAGAAGTAAA+TGG	+	chr8_4:52409592-52409611	Msa1332450:intron	20.0%
!!	AAATAAAAAGTAGAAGTCAT+AGG	-	chr8_4:52409889-52409908	None:intergenic	20.0%
!!	TGCATGTTTATTTATATGAA+TGG	+	chr8_4:52409908-52409927	Msa1332450:intron	20.0%
!!!	TATTTTCTTTTGACTATAAC+TGG	-	chr8_4:52410370-52410389	Msa1332460:three_prime_UTR	20.0%
!!!	ATTGGTATATATTTTTGTGT+AGG	+	chr8_4:52410462-52410481	Msa1332450:intron	20.0%
!	TACAAGACATAAAGAAGAAA+AGG	-	chr8_4:52409226-52409245	None:intergenic	25.0%
!!!	ATTTTTTTTTGGGGGTATAT+AGG	+	chr8_4:52409539-52409558	Msa1332450:intron	25.0%
!!!	TTTTTTTTTGGGGGTATATA+GGG	+	chr8_4:52409540-52409559	Msa1332450:intron	25.0%
!	AGAAAACACAAATCCTTTAT+AGG	+	chr8_4:52409705-52409724	Msa1332450:intron	25.0%
!	TCATTAGTGAAAACCTATAA+AGG	-	chr8_4:52409721-52409740	None:intergenic	25.0%
!	TGATAAAATTAATGCAGCAA+AGG	+	chr8_4:52410340-52410359	Msa1332450:CDS	25.0%
!	GCAAAACAATTGAAAAGTTA+TGG	-	chr8_4:52410672-52410691	Msa1332460:three_prime_UTR	25.0%
!	AAAAAATAAGAAAAGGAGAC+AGG	-	chr8_4:52410788-52410807	Msa1332460:three_prime_UTR	25.0%
!	TACAAGACATAAAGAAGAAA+AGG	-	chr8_4:52409226-52409245	None:intergenic	25.0%
!!!	ATTTTTTTTTGGGGGTATAT+AGG	+	chr8_4:52409539-52409558	Msa1332450:intron	25.0%
!!!	TTTTTTTTTGGGGGTATATA+GGG	+	chr8_4:52409540-52409559	Msa1332450:intron	25.0%
!	AGAAAACACAAATCCTTTAT+AGG	+	chr8_4:52409705-52409724	Msa1332450:intron	25.0%
!	TCATTAGTGAAAACCTATAA+AGG	-	chr8_4:52409721-52409740	None:intergenic	25.0%
!	TGATAAAATTAATGCAGCAA+AGG	+	chr8_4:52410340-52410359	Msa1332450:CDS	25.0%
!	GCAAAACAATTGAAAAGTTA+TGG	-	chr8_4:52410672-52410691	Msa1332460:three_prime_UTR	25.0%
!	AAAAAATAAGAAAAGGAGAC+AGG	-	chr8_4:52410788-52410807	Msa1332460:three_prime_UTR	25.0%
	ATATGCCGACAAAATTTGAT+TGG	-	chr8_4:52409169-52409188	None:intergenic	30.0%
	TTATGTCTTGTAAGTACTTC+TGG	+	chr8_4:52409234-52409253	Msa1332450:intron	30.0%
!	TTTTCACAATACCAATTGTG+TGG	+	chr8_4:52409271-52409290	Msa1332450:intron	30.0%
	TGATTCCTGATAGTACATAA+AGG	+	chr8_4:52409501-52409520	Msa1332450:intron	30.0%
	GATTCCTGATAGTACATAAA+GGG	+	chr8_4:52409502-52409521	Msa1332450:intron	30.0%
!!!	TTTTTTTTGGGGGTATATAG+GGG	+	chr8_4:52409541-52409560	Msa1332450:intron	30.0%
	AAGAAAGGAAACTAACTAAG+TGG	-	chr8_4:52409654-52409673	None:intergenic	30.0%
!!!	GTTTCCTTTCTTATTTTCCT+TGG	+	chr8_4:52409662-52409681	Msa1332450:intron	30.0%
	AATTGTATTACATTCGACCA+AGG	-	chr8_4:52409682-52409701	None:intergenic	30.0%
!	ATCAAGTTTTCAGACATTTC+TGG	+	chr8_4:52409771-52409790	Msa1332450:intron	30.0%
	TAAGTCATGTGAATTTGAGA+AGG	+	chr8_4:52410192-52410211	Msa1332450:CDS	30.0%
	CATAATCACATTATCATCCT+TGG	-	chr8_4:52410240-52410259	Msa1332460:three_prime_UTR	30.0%
!	TGATTATGCATTTGAGATGT+TGG	+	chr8_4:52410252-52410271	Msa1332450:CDS	30.0%
	TTCACGTGTAGATTAAGTAT+TGG	+	chr8_4:52410444-52410463	Msa1332450:intron	30.0%
!	TTTTCAAAAGGACTTAAACC+TGG	+	chr8_4:52410501-52410520	Msa1332450:CDS	30.0%
	TGAAGCTGACCAAAAAATTT+AGG	+	chr8_4:52410642-52410661	Msa1332450:CDS	30.0%
!!!	TATGGCTTGCCTAAATTTTT+TGG	-	chr8_4:52410654-52410673	Msa1332460:three_prime_UTR	30.0%
	ACCAACAAAACTTCTCTTTA+TGG	-	chr8_4:52410713-52410732	Msa1332460:three_prime_UTR	30.0%
	GTACGAAAATAGAAAAGCAA+GGG	-	chr8_4:52410857-52410876	Msa1332460:three_prime_UTR	30.0%
	TGTACGAAAATAGAAAAGCA+AGG	-	chr8_4:52410858-52410877	Msa1332460:three_prime_UTR	30.0%
	ATATGCCGACAAAATTTGAT+TGG	-	chr8_4:52409169-52409188	None:intergenic	30.0%
	TTATGTCTTGTAAGTACTTC+TGG	+	chr8_4:52409234-52409253	Msa1332450:intron	30.0%
!	TTTTCACAATACCAATTGTG+TGG	+	chr8_4:52409271-52409290	Msa1332450:intron	30.0%
	TGATTCCTGATAGTACATAA+AGG	+	chr8_4:52409501-52409520	Msa1332450:intron	30.0%
	GATTCCTGATAGTACATAAA+GGG	+	chr8_4:52409502-52409521	Msa1332450:intron	30.0%
!!!	TTTTTTTTGGGGGTATATAG+GGG	+	chr8_4:52409541-52409560	Msa1332450:intron	30.0%
	AAGAAAGGAAACTAACTAAG+TGG	-	chr8_4:52409654-52409673	None:intergenic	30.0%
!!!	GTTTCCTTTCTTATTTTCCT+TGG	+	chr8_4:52409662-52409681	Msa1332450:intron	30.0%
	AATTGTATTACATTCGACCA+AGG	-	chr8_4:52409682-52409701	None:intergenic	30.0%
!	ATCAAGTTTTCAGACATTTC+TGG	+	chr8_4:52409771-52409790	Msa1332450:intron	30.0%
	TAAGTCATGTGAATTTGAGA+AGG	+	chr8_4:52410192-52410211	Msa1332450:CDS	30.0%
	CATAATCACATTATCATCCT+TGG	-	chr8_4:52410240-52410259	Msa1332460:three_prime_UTR	30.0%
!	TGATTATGCATTTGAGATGT+TGG	+	chr8_4:52410252-52410271	Msa1332450:CDS	30.0%
	TTCACGTGTAGATTAAGTAT+TGG	+	chr8_4:52410444-52410463	Msa1332450:intron	30.0%
!	TTTTCAAAAGGACTTAAACC+TGG	+	chr8_4:52410501-52410520	Msa1332450:CDS	30.0%
	TGAAGCTGACCAAAAAATTT+AGG	+	chr8_4:52410642-52410661	Msa1332450:CDS	30.0%
!!!	TATGGCTTGCCTAAATTTTT+TGG	-	chr8_4:52410654-52410673	Msa1332460:three_prime_UTR	30.0%
	ACCAACAAAACTTCTCTTTA+TGG	-	chr8_4:52410713-52410732	Msa1332460:three_prime_UTR	30.0%
	GTACGAAAATAGAAAAGCAA+GGG	-	chr8_4:52410857-52410876	Msa1332460:three_prime_UTR	30.0%
	TGTACGAAAATAGAAAAGCA+AGG	-	chr8_4:52410858-52410877	Msa1332460:three_prime_UTR	30.0%
	GAAAAACATAAGCACTCACT+GGG	-	chr8_4:52409204-52409223	None:intergenic	35.0%
!!!	CTTGTAAGTACTTCTGGTTT+TGG	+	chr8_4:52409240-52409259	Msa1332450:intron	35.0%
	AAGATCCGAAGGCTTATATT+CGG	+	chr8_4:52409373-52409392	Msa1332450:intron	35.0%
	AAAGTCCGAATATAAGCCTT+CGG	-	chr8_4:52409381-52409400	None:intergenic	35.0%
	CTGCAACTGCAATGAATTAT+TGG	+	chr8_4:52409466-52409485	Msa1332450:intron	35.0%
	TATCAGGAATCAGATTCCTT+GGG	-	chr8_4:52409493-52409512	None:intergenic	35.0%
	ACATCCCTTTATGTACTATC+AGG	-	chr8_4:52409509-52409528	None:intergenic	35.0%
	GGGTATTATACATACATAGC+AGG	+	chr8_4:52409561-52409580	Msa1332450:intron	35.0%
	ATTATACATACATAGCAGGC+AGG	+	chr8_4:52409565-52409584	Msa1332450:intron	35.0%
	TCGACCAAGGAAAATAAGAA+AGG	-	chr8_4:52409669-52409688	None:intergenic	35.0%
!	CTCCTTTTCCTCAATCAAAA+CGG	-	chr8_4:52410047-52410066	None:intergenic	35.0%
	ATCCAAGTGCTAATTCAAAG+TGG	-	chr8_4:52410127-52410146	None:intergenic	35.0%
!	GTCAACTGAAAGATGGTTTT+TGG	+	chr8_4:52410214-52410233	Msa1332450:CDS	35.0%
	TTTATCAGTGTTACACAGTC+TGG	+	chr8_4:52410412-52410431	Msa1332450:intron	35.0%
	TTATCAGTGTTACACAGTCT+GGG	+	chr8_4:52410413-52410432	Msa1332450:intron	35.0%
!!	TGAAGCAGCTGATTTTCAAA+AGG	+	chr8_4:52410489-52410508	Msa1332450:CDS	35.0%
	TTTCTCATCAGGAATTTCGA+TGG	-	chr8_4:52410575-52410594	Msa1332460:three_prime_UTR	35.0%
!!!	AATTTTTTGGTCAGCTTCAG+AGG	-	chr8_4:52410641-52410660	Msa1332460:three_prime_UTR	35.0%
!!	GCCATAAAGAGAAGTTTTGT+TGG	+	chr8_4:52410709-52410728	Msa1332450:three_prime_UTR	35.0%
!!!	AGCAAGGGAATATTTTGATC+AGG	-	chr8_4:52410842-52410861	Msa1332460:three_prime_UTR	35.0%
	GAAAAACATAAGCACTCACT+GGG	-	chr8_4:52409204-52409223	None:intergenic	35.0%
!!!	CTTGTAAGTACTTCTGGTTT+TGG	+	chr8_4:52409240-52409259	Msa1332450:intron	35.0%
	AAGATCCGAAGGCTTATATT+CGG	+	chr8_4:52409373-52409392	Msa1332450:intron	35.0%
	AAAGTCCGAATATAAGCCTT+CGG	-	chr8_4:52409381-52409400	None:intergenic	35.0%
	CTGCAACTGCAATGAATTAT+TGG	+	chr8_4:52409466-52409485	Msa1332450:intron	35.0%
	TATCAGGAATCAGATTCCTT+GGG	-	chr8_4:52409493-52409512	None:intergenic	35.0%
	ACATCCCTTTATGTACTATC+AGG	-	chr8_4:52409509-52409528	None:intergenic	35.0%
	GGGTATTATACATACATAGC+AGG	+	chr8_4:52409561-52409580	Msa1332450:intron	35.0%
	ATTATACATACATAGCAGGC+AGG	+	chr8_4:52409565-52409584	Msa1332450:intron	35.0%
	TCGACCAAGGAAAATAAGAA+AGG	-	chr8_4:52409669-52409688	None:intergenic	35.0%
!	CTCCTTTTCCTCAATCAAAA+CGG	-	chr8_4:52410047-52410066	None:intergenic	35.0%
	ATCCAAGTGCTAATTCAAAG+TGG	-	chr8_4:52410127-52410146	None:intergenic	35.0%
!	GTCAACTGAAAGATGGTTTT+TGG	+	chr8_4:52410214-52410233	Msa1332450:CDS	35.0%
	TTTATCAGTGTTACACAGTC+TGG	+	chr8_4:52410412-52410431	Msa1332450:intron	35.0%
	TTATCAGTGTTACACAGTCT+GGG	+	chr8_4:52410413-52410432	Msa1332450:intron	35.0%
!!	TGAAGCAGCTGATTTTCAAA+AGG	+	chr8_4:52410489-52410508	Msa1332450:CDS	35.0%
	TTTCTCATCAGGAATTTCGA+TGG	-	chr8_4:52410575-52410594	Msa1332460:three_prime_UTR	35.0%
!!!	AATTTTTTGGTCAGCTTCAG+AGG	-	chr8_4:52410641-52410660	Msa1332460:three_prime_UTR	35.0%
!!	GCCATAAAGAGAAGTTTTGT+TGG	+	chr8_4:52410709-52410728	Msa1332450:three_prime_UTR	35.0%
!!!	AGCAAGGGAATATTTTGATC+AGG	-	chr8_4:52410842-52410861	Msa1332460:three_prime_UTR	35.0%
	GGAAAAACATAAGCACTCAC+TGG	-	chr8_4:52409205-52409224	None:intergenic	40.0%
	TCACAATACCAATTGTGTGG+TGG	+	chr8_4:52409274-52409293	Msa1332450:intron	40.0%
	ATCAGGAATCAGATTCCTTG+GGG	-	chr8_4:52409492-52409511	None:intergenic	40.0%
	CTATCAGGAATCAGATTCCT+TGG	-	chr8_4:52409494-52409513	None:intergenic	40.0%
!!!	TCTATGCACCGTTTTGATTG+AGG	+	chr8_4:52410036-52410055	Msa1332450:five_prime_UTR	40.0%
!!!	CACCGTTTTGATTGAGGAAA+AGG	+	chr8_4:52410042-52410061	Msa1332450:five_prime_UTR	40.0%
!!!	CGTTTTGATTGAGGAAAAGG+AGG	+	chr8_4:52410045-52410064	Msa1332450:five_prime_UTR	40.0%
!!	TGCCACTTTGAATTAGCACT+TGG	+	chr8_4:52410122-52410141	Msa1332450:five_prime_UTR	40.0%
	TGAGAAGGTCAACTGAAAGA+TGG	+	chr8_4:52410207-52410226	Msa1332450:CDS	40.0%
!!!	TGAAAGATGGTTTTTGGCCA+AGG	+	chr8_4:52410220-52410239	Msa1332450:CDS	40.0%
	CTGATTACTCGGTCTAGTAT+CGG	-	chr8_4:52410305-52410324	Msa1332460:three_prime_UTR	40.0%
	TCTTGACCAGTCTGATTACT+CGG	-	chr8_4:52410316-52410335	Msa1332460:three_prime_UTR	40.0%
	GGATAGGGTTGAGCTTATTA+TGG	+	chr8_4:52410531-52410550	Msa1332450:CDS	40.0%
	AGCTTATCTGCTTTCTCATC+AGG	-	chr8_4:52410586-52410605	Msa1332460:three_prime_UTR	40.0%
	GGCTATGTACTTTGCAACAT+CGG	-	chr8_4:52410620-52410639	Msa1332460:three_prime_UTR	40.0%
!!	GTTTTGTTGGTTGAGCTGTT+TGG	+	chr8_4:52410722-52410741	Msa1332450:three_prime_UTR	40.0%
!	TCAGGTGTCATGCTTACATT+GGG	-	chr8_4:52410824-52410843	Msa1332460:three_prime_UTR	40.0%
!	ATCAGGTGTCATGCTTACAT+TGG	-	chr8_4:52410825-52410844	Msa1332460:three_prime_UTR	40.0%
	GGAAAAACATAAGCACTCAC+TGG	-	chr8_4:52409205-52409224	None:intergenic	40.0%
	TCACAATACCAATTGTGTGG+TGG	+	chr8_4:52409274-52409293	Msa1332450:intron	40.0%
	ATCAGGAATCAGATTCCTTG+GGG	-	chr8_4:52409492-52409511	None:intergenic	40.0%
	CTATCAGGAATCAGATTCCT+TGG	-	chr8_4:52409494-52409513	None:intergenic	40.0%
!!!	TCTATGCACCGTTTTGATTG+AGG	+	chr8_4:52410036-52410055	Msa1332450:five_prime_UTR	40.0%
!!!	CACCGTTTTGATTGAGGAAA+AGG	+	chr8_4:52410042-52410061	Msa1332450:five_prime_UTR	40.0%
!!!	CGTTTTGATTGAGGAAAAGG+AGG	+	chr8_4:52410045-52410064	Msa1332450:five_prime_UTR	40.0%
!!	TGCCACTTTGAATTAGCACT+TGG	+	chr8_4:52410122-52410141	Msa1332450:five_prime_UTR	40.0%
	TGAGAAGGTCAACTGAAAGA+TGG	+	chr8_4:52410207-52410226	Msa1332450:CDS	40.0%
!!!	TGAAAGATGGTTTTTGGCCA+AGG	+	chr8_4:52410220-52410239	Msa1332450:CDS	40.0%
	CTGATTACTCGGTCTAGTAT+CGG	-	chr8_4:52410305-52410324	Msa1332460:three_prime_UTR	40.0%
	TCTTGACCAGTCTGATTACT+CGG	-	chr8_4:52410316-52410335	Msa1332460:three_prime_UTR	40.0%
	GGATAGGGTTGAGCTTATTA+TGG	+	chr8_4:52410531-52410550	Msa1332450:CDS	40.0%
	AGCTTATCTGCTTTCTCATC+AGG	-	chr8_4:52410586-52410605	Msa1332460:three_prime_UTR	40.0%
	GGCTATGTACTTTGCAACAT+CGG	-	chr8_4:52410620-52410639	Msa1332460:three_prime_UTR	40.0%
!!	GTTTTGTTGGTTGAGCTGTT+TGG	+	chr8_4:52410722-52410741	Msa1332450:three_prime_UTR	40.0%
!	TCAGGTGTCATGCTTACATT+GGG	-	chr8_4:52410824-52410843	Msa1332460:three_prime_UTR	40.0%
!	ATCAGGTGTCATGCTTACAT+TGG	-	chr8_4:52410825-52410844	Msa1332460:three_prime_UTR	40.0%
	AGCCTTCGGATCTTCAATCA+GGG	-	chr8_4:52409367-52409386	None:intergenic	45.0%
	AAGCCTTCGGATCTTCAATC+AGG	-	chr8_4:52409368-52409387	None:intergenic	45.0%
	GCAATGAATTATTGGCCCCA+AGG	+	chr8_4:52409474-52409493	Msa1332450:intron	45.0%
	ACTAGACCGAGTAATCAGAC+TGG	+	chr8_4:52410307-52410326	Msa1332450:CDS	45.0%
	TAAACCTGGACAGCTTGGAT+AGG	+	chr8_4:52410515-52410534	Msa1332450:CDS	45.0%
	AAACCTGGACAGCTTGGATA+GGG	+	chr8_4:52410516-52410535	Msa1332450:CDS	45.0%
	TGTCATGCTTACATTGGGGT+GGG	-	chr8_4:52410819-52410838	Msa1332460:three_prime_UTR	45.0%
!	CAGGTGTCATGCTTACATTG+GGG	-	chr8_4:52410823-52410842	Msa1332460:three_prime_UTR	45.0%
	AGCCTTCGGATCTTCAATCA+GGG	-	chr8_4:52409367-52409386	None:intergenic	45.0%
	AAGCCTTCGGATCTTCAATC+AGG	-	chr8_4:52409368-52409387	None:intergenic	45.0%
	GCAATGAATTATTGGCCCCA+AGG	+	chr8_4:52409474-52409493	Msa1332450:intron	45.0%
	ACTAGACCGAGTAATCAGAC+TGG	+	chr8_4:52410307-52410326	Msa1332450:CDS	45.0%
	TAAACCTGGACAGCTTGGAT+AGG	+	chr8_4:52410515-52410534	Msa1332450:CDS	45.0%
	AAACCTGGACAGCTTGGATA+GGG	+	chr8_4:52410516-52410535	Msa1332450:CDS	45.0%
	TGTCATGCTTACATTGGGGT+GGG	-	chr8_4:52410819-52410838	Msa1332460:three_prime_UTR	45.0%
!	CAGGTGTCATGCTTACATTG+GGG	-	chr8_4:52410823-52410842	Msa1332460:three_prime_UTR	45.0%
	CACTCACTGGGAGATTCGTT+TGG	-	chr8_4:52409192-52409211	None:intergenic	50.0%
	TCTGCGTACCACCACACAAT+TGG	-	chr8_4:52409285-52409304	None:intergenic	50.0%
	TCCCCTGATTGAAGATCCGA+AGG	+	chr8_4:52409362-52409381	Msa1332450:intron	50.0%
	GCCTTCGGATCTTCAATCAG+GGG	-	chr8_4:52409366-52409385	None:intergenic	50.0%
!	AGATGTTGGTGCTCTTCAGC+AGG	+	chr8_4:52410266-52410285	Msa1332450:CDS	50.0%
!	GTCTAGTATCGGATCAGAGC+TGG	-	chr8_4:52410294-52410313	Msa1332460:three_prime_UTR	50.0%
	GGACTTAAACCTGGACAGCT+TGG	+	chr8_4:52410510-52410529	Msa1332450:CDS	50.0%
	GTCATGCTTACATTGGGGTG+GGG	-	chr8_4:52410818-52410837	Msa1332460:three_prime_UTR	50.0%
	GTGTCATGCTTACATTGGGG+TGG	-	chr8_4:52410820-52410839	Msa1332460:three_prime_UTR	50.0%
	CACTCACTGGGAGATTCGTT+TGG	-	chr8_4:52409192-52409211	None:intergenic	50.0%
	TCTGCGTACCACCACACAAT+TGG	-	chr8_4:52409285-52409304	None:intergenic	50.0%
	TCCCCTGATTGAAGATCCGA+AGG	+	chr8_4:52409362-52409381	Msa1332450:intron	50.0%
	GCCTTCGGATCTTCAATCAG+GGG	-	chr8_4:52409366-52409385	None:intergenic	50.0%
!	AGATGTTGGTGCTCTTCAGC+AGG	+	chr8_4:52410266-52410285	Msa1332450:CDS	50.0%
!	GTCTAGTATCGGATCAGAGC+TGG	-	chr8_4:52410294-52410313	Msa1332460:three_prime_UTR	50.0%
	GGACTTAAACCTGGACAGCT+TGG	+	chr8_4:52410510-52410529	Msa1332450:CDS	50.0%
	GTCATGCTTACATTGGGGTG+GGG	-	chr8_4:52410818-52410837	Msa1332460:three_prime_UTR	50.0%
	GTGTCATGCTTACATTGGGG+TGG	-	chr8_4:52410820-52410839	Msa1332460:three_prime_UTR	50.0%
	CAACCCTATCCAAGCTGTCC+AGG	-	chr8_4:52410522-52410541	Msa1332460:three_prime_UTR	55.0%
	CAACCCTATCCAAGCTGTCC+AGG	-	chr8_4:52410522-52410541	Msa1332460:three_prime_UTR	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	52409134	52410899	52409134	ID=Msa1332450;Name=Msa1332450
chr8_4	mRNA	52409134	52410899	52409134	ID=Msa1332450-mRNA-1;Parent=Msa1332450;Name=Msa1332450-mRNA-1;_AED=0.01;_eAED=0.01;_QI=204\|1\|1\|1\|0.5\|0.33\|3\|236\|124
chr8_4	exon	52409134	52409204	52409134	ID=Msa1332450-mRNA-1:exon:15718;Parent=Msa1332450-mRNA-1
chr8_4	exon	52410034	52410361	52410034	ID=Msa1332450-mRNA-1:exon:15719;Parent=Msa1332450-mRNA-1
chr8_4	exon	52410484	52410899	52410484	ID=Msa1332450-mRNA-1:exon:15720;Parent=Msa1332450-mRNA-1
chr8_4	five_prime_UTR	52409134	52409204	52409134	ID=Msa1332450-mRNA-1:five_prime_utr;Parent=Msa1332450-mRNA-1
chr8_4	five_prime_UTR	52410034	52410166	52410034	ID=Msa1332450-mRNA-1:five_prime_utr;Parent=Msa1332450-mRNA-1
chr8_4	CDS	52410167	52410361	52410167	ID=Msa1332450-mRNA-1:cds;Parent=Msa1332450-mRNA-1
chr8_4	CDS	52410484	52410663	52410484	ID=Msa1332450-mRNA-1:cds;Parent=Msa1332450-mRNA-1
chr8_4	three_prime_UTR	52410664	52410899	52410664	ID=Msa1332450-mRNA-1:three_prime_utr;Parent=Msa1332450-mRNA-1

Gene Sequence	>Msa1332450 ATGCAAAGCATAAAGAAATCTATACTAAGTCATGTGAATTTGAGAAGGTCAACTGAAAGATGGTTTTTGGCCAAGGATGATAATGTGATTATGCATTTGAGATGTTGGTGCTCTTCAGCAGGTGCCAGCTCTGATCCGATACTAGACCGAGTAATCAGACTGGTCAAGAAATATGATAAAATTAATGCAGCAAAGGTTACTGAAGCAGCTGATTTTCAAAAGGACTTAAACCTGGACAGCTTGGATAGGGTTGAGCTTATTATGGCTCTTGAAGAAGAATTTTCCATCGAAATTCCTGATGAGAAAGCAGATAAGCTTACTTGCTGTACCGATGTTGCAAAGTACATAGCCTCTGAAGCTGACCAAAAAATTTAG
Protein sequence	>Msa1332450 MQSIKKSILSHVNLRRSTERWFLAKDDNVIMHLRCWCSSAGASSDPILDRVIRLVKKYDKINAAKVTEAADFQKDLNLDSLDRVELIMALEEEFSIEIPDEKADKLTCCTDVAKYIASEADQKI*