AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0168390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0168390 MtrunA17_Chr1g0184791 97.107 242 6 1 3 243 4 245 1.25e-174 480
Msa0168390 MtrunA17_Chr7g0260641 70.312 256 57 7 1 243 1 250 3.86e-109 314
Msa0168390 MtrunA17_Chr1g0187921 66.390 241 56 7 14 243 10 236 1.15e-97 285
Msa0168390 MtrunA17_Chr1g0190711 63.934 244 67 6 14 243 17 253 6.83e-93 273
Msa0168390 MtrunA17_Chr5g0411501 64.497 169 55 4 77 243 170 335 7.79e-69 215
Msa0168390 MtrunA17_Chr5g0428141 65.217 161 52 3 83 243 191 347 2.24e-68 214
Msa0168390 MtrunA17_Chr6g0486121 53.810 210 83 4 35 243 58 254 1.34e-67 209
Msa0168390 MtrunA17_Chr8g0366881 64.671 167 53 4 79 243 186 348 2.04e-67 211
Msa0168390 MtrunA17_Chr2g0331411 64.417 163 53 4 83 243 167 326 3.57e-66 207
Msa0168390 MtrunA17_Chr8g0390281 61.677 167 55 4 81 243 132 293 1.80e-64 202
Msa0168390 MtrunA17_Chr1g0184801 42.500 240 92 6 1 232 1 202 3.19e-51 166
Msa0168390 MtrunA17_Chr1g0166011 44.105 229 74 7 12 232 1 183 3.03e-50 162
Msa0168390 MtrunA17_Chr7g0260621 43.162 234 86 6 9 232 6 202 1.18e-48 159
Msa0168390 MtrunA17_Chr1g0187961 41.304 230 69 7 9 231 8 178 3.28e-47 154
Msa0168390 MtrunA17_Chr4g0004181 50.000 152 43 6 86 235 52 172 3.81e-35 123
Msa0168390 MtrunA17_Chr2g0332141 57.426 101 32 4 126 223 8 100 3.60e-32 114
Msa0168390 MtrunA17_Chr1g0183251 36.078 255 108 7 21 238 27 263 2.84e-30 113
Msa0168390 MtrunA17_Chr2g0330951 33.460 263 138 8 6 234 4 263 4.81e-30 112
Msa0168390 MtrunA17_Chr8g0340731 43.226 155 72 4 85 224 104 257 5.70e-30 112
Msa0168390 MtrunA17_Chr4g0069561 37.821 156 61 7 80 228 58 184 9.59e-20 83.6
Msa0168390 MtrunA17_Chr3g0137601 30.986 142 78 3 88 228 88 210 3.07e-19 83.2
Msa0168390 MtrunA17_Chr1g0193861 38.835 103 50 1 122 224 81 170 3.62e-17 76.6
Msa0168390 MtrunA17_Chr4g0064131 30.435 138 73 3 89 226 93 207 2.28e-16 75.1
Msa0168390 MtrunA17_Chr7g0270851 37.374 99 49 1 126 224 70 155 9.64e-16 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0168390 AT4G14550.1 65.432 243 58 8 12 243 1 228 3.39e-96 281
Msa0168390 AT3G23050.1 65.702 242 53 9 21 243 13 243 4.29e-95 279
Msa0168390 AT3G04730.1 61.382 246 66 7 14 243 4 236 6.03e-93 273
Msa0168390 AT4G14550.2 63.793 232 58 8 12 232 1 217 1.24e-87 259
Msa0168390 AT1G04250.1 61.765 238 60 9 18 243 11 229 4.44e-87 258
Msa0168390 AT4G14550.3 62.381 210 53 8 12 210 1 195 5.30e-74 223
Msa0168390 AT3G23050.2 62.679 209 49 8 21 211 13 210 1.37e-72 220
Msa0168390 AT4G29080.1 60.106 188 62 4 63 242 122 304 8.02e-68 211
Msa0168390 AT5G65670.2 54.751 221 84 6 35 243 120 336 1.83e-67 212
Msa0168390 AT5G65670.1 54.260 223 84 7 35 243 120 338 1.96e-65 206
Msa0168390 AT2G22670.3 59.259 162 61 4 83 242 160 318 1.53e-60 193
Msa0168390 AT2G22670.1 59.259 162 61 4 83 242 160 318 1.53e-60 193
Msa0168390 AT2G22670.4 59.259 162 61 4 83 242 177 335 1.91e-60 194
Msa0168390 AT2G22670.5 58.025 162 61 5 83 242 160 316 2.92e-57 185
Msa0168390 AT2G22670.2 58.025 162 61 5 83 242 160 316 2.92e-57 185
Msa0168390 AT5G43700.1 58.000 150 37 5 84 232 58 182 3.48e-50 162
Msa0168390 AT1G04240.1 46.222 225 80 9 9 232 2 186 1.04e-48 159
Msa0168390 AT3G23030.1 54.362 149 35 2 83 231 55 170 2.10e-48 157
Msa0168390 AT3G23030.2 54.362 149 35 2 83 231 142 257 1.63e-47 158
Msa0168390 AT1G04240.2 46.222 225 80 9 9 232 102 286 1.77e-47 159
Msa0168390 AT4G14550.4 58.182 165 44 7 12 165 1 151 2.35e-45 150
Msa0168390 AT3G23050.3 57.317 164 42 7 21 166 13 166 3.33e-43 144
Msa0168390 AT3G15540.1 39.662 237 96 8 8 238 2 197 1.40e-40 138
Msa0168390 AT4G14560.1 45.833 216 56 7 14 228 9 164 2.79e-40 137
Msa0168390 AT2G33310.3 44.211 190 84 5 65 237 52 236 4.05e-40 139
Msa0168390 AT2G33310.1 44.211 190 84 5 65 237 52 236 4.05e-40 139
Msa0168390 AT1G04550.2 43.814 194 80 5 65 238 46 230 1.63e-39 137
Msa0168390 AT2G33310.2 43.979 191 84 6 65 237 52 237 1.23e-38 135
Msa0168390 AT1G15580.1 40.094 212 62 6 14 224 10 157 1.10e-37 130
Msa0168390 AT1G52830.1 36.207 232 91 8 9 232 3 185 3.97e-33 119
Msa0168390 AT3G16500.1 41.143 175 87 4 66 227 81 252 4.62e-33 121
Msa0168390 AT3G16500.2 41.143 175 87 4 66 227 81 252 4.80e-33 121
Msa0168390 AT4G28640.1 44.375 160 71 5 87 232 87 242 1.29e-32 119
Msa0168390 AT4G28640.3 44.304 158 70 5 87 230 87 240 2.31e-32 119
Msa0168390 AT4G28640.2 44.304 158 70 5 87 230 87 240 2.98e-31 117
Msa0168390 AT1G04100.1 41.176 170 69 7 90 232 95 260 1.56e-27 106
Msa0168390 AT1G51950.1 38.415 164 74 5 85 227 93 250 3.95e-27 105
Msa0168390 AT5G25890.1 37.838 148 68 2 87 234 47 170 6.41e-26 100
Msa0168390 AT3G17600.1 31.875 160 78 4 69 224 22 154 8.17e-21 86.3
Msa0168390 AT3G62100.1 38.532 109 51 2 118 224 74 168 2.57e-17 77.4
Msa0168390 AT2G46990.1 40.404 99 45 1 126 224 86 170 4.23e-17 76.6
Msa0168390 AT4G32280.2 31.579 114 53 3 126 239 119 207 4.67e-15 72.0
Msa0168390 AT4G32280.1 25.824 182 81 6 87 239 93 249 4.91e-15 72.8
Msa0168390 AT2G46990.2 40.698 86 37 1 126 211 86 157 2.80e-12 63.2
Msa0168390 AT3G62100.2 37.500 96 44 2 118 211 74 155 4.52e-12 62.8
Msa0168390 AT1G04550.1 35.246 122 60 3 65 167 46 167 5.18e-12 62.8
Msa0168390 AT1G15050.2 29.630 135 76 3 95 229 35 150 3.94e-11 60.1
Msa0168390 AT1G15050.1 29.630 135 76 3 95 229 67 182 8.40e-11 59.7

Find 100 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATCCTTCACTGCTCTTT+TGG 0.116525 1_4:+50949821 None:intergenic
CTAACTTTCACAAAGCTTAT+TGG 0.117785 1_4:+50949769 None:intergenic
TTTAGAGTCATTATCGTTTC+TGG 0.134360 1_4:+50947712 None:intergenic
TATTTAATTTGCATAGAAAT+TGG 0.213907 1_4:-50947462 Msa0168390:three_prime_UTR
GGTAAAGGAGTTAAACATTT+TGG 0.221116 1_4:+50949669 None:intergenic
GCTCTTCTGCTCTCTGATTC+TGG 0.233246 1_4:-50950774 Msa0168390:CDS
AATCCACCTCCACCATGTTT+AGG 0.296733 1_4:+50947395 None:intergenic
GCTTCAGTTGCCAAGTCAAC+TGG 0.308202 1_4:+50947887 None:intergenic
TTGTGAATCCCAAGGCATTA+AGG 0.313394 1_4:-50948981 Msa0168390:CDS
CATGGTGGAGGTGGATTGAT+TGG 0.322396 1_4:-50947389 Msa0168390:three_prime_UTR
TGTGTATGTGTAACATTATA+TGG 0.326644 1_4:-50947798 Msa0168390:three_prime_UTR
ACATCTTGAGGTCTACTTTA+CGG 0.340864 1_4:+50949725 None:intergenic
GGAGGTGGATTGATTGGGTG+GGG 0.344659 1_4:-50947383 Msa0168390:three_prime_UTR
GGAAAACAACAGAAGTAATA+AGG 0.347716 1_4:-50950911 Msa0168390:CDS
ATCCACCTCCACCATGTTTA+GGG 0.351865 1_4:+50947396 None:intergenic
GGAAGTTGCAGGGCTGAAAA+AGG 0.353058 1_4:-50950988 Msa0168390:CDS
AGCAGAAGAGCCTTCTCCTT+TGG 0.360991 1_4:+50950786 None:intergenic
CCATCTTTGTCTTCATAAGT+TGG 0.383939 1_4:+50948902 None:intergenic
CATGAAATCCTTAATGCCTT+GGG 0.384985 1_4:+50948973 None:intergenic
GCATCACATGGAGCAGGTTA+TGG 0.392191 1_4:+50947910 None:intergenic
GTGGAGGTGGATTGATTGGG+TGG 0.398888 1_4:-50947385 Msa0168390:three_prime_UTR
GAAGCTTAATCTTTCTACTA+AGG 0.400977 1_4:-50950835 Msa0168390:CDS
TTCTGGTCATAATTTATACT+TGG 0.403351 1_4:+50947729 None:intergenic
TCATGAAATCCTTAATGCCT+TGG 0.406074 1_4:+50948972 None:intergenic
CATCTTTGTCTTCATAAGTT+GGG 0.414636 1_4:+50948903 None:intergenic
AGGCTAGGACTTGGATTGCC+AGG 0.417824 1_4:-50950933 Msa0168390:CDS
TTCTCCTTTGGCTTAAATTG+TGG 0.418936 1_4:+50950798 None:intergenic
AGCATTCAGTGTGATTTGAT+TGG 0.424793 1_4:+50951054 None:intergenic
AACTTGATAATGGAAGTTGC+AGG 0.451323 1_4:-50950999 Msa0168390:exon
GAGGTGGATTGATTGGGTGG+GGG 0.454102 1_4:-50947382 Msa0168390:three_prime_UTR
GAATGAGAGTAATAAGTTGA+TGG 0.457906 1_4:-50948951 Msa0168390:CDS
GGAAAGGAAGCCATTGGACT+AGG 0.462382 1_4:-50948557 Msa0168390:intron
GCTAAGGCATCAGAGAGCTC+TGG 0.465095 1_4:+50949691 None:intergenic
GTCCCTAAACATGGTGGAGG+TGG 0.468896 1_4:-50947398 Msa0168390:three_prime_UTR
GGAGGAAAAGATGGTGTTTG+AGG 0.477721 1_4:-50950967 Msa0168390:CDS
GGGCAGCAAACATGTTCTTA+CGG 0.483487 1_4:+50949842 None:intergenic
CTTAATCTTTCTACTAAGGA+AGG 0.487124 1_4:-50950831 Msa0168390:CDS
TAATGAACAACTTACTTAGC+AGG 0.499421 1_4:+50950741 None:intergenic
TTTGTGACAATAATAGAGGA+AGG 0.505887 1_4:+50951077 None:intergenic
AAGAAAATTTAAAGACAACT+TGG 0.509922 1_4:+50947572 None:intergenic
TGGAGGTGGATTGATTGGGT+GGG 0.511444 1_4:-50947384 Msa0168390:three_prime_UTR
ATAGAGGAAGGGGTATAGAA+AGG 0.512057 1_4:+50951089 None:intergenic
AGGCAAGAGCACGTTGAAGT+GGG 0.512186 1_4:+50951109 None:intergenic
AAAATCATTAATTTAATACA+AGG 0.512218 1_4:+50947524 None:intergenic
TAAGCTGCAGCAGAAACATA+TGG 0.513103 1_4:-50947859 Msa0168390:three_prime_UTR
CATGTGATGCCCAGTTGACT+TGG 0.514344 1_4:-50947897 Msa0168390:three_prime_UTR
CTAAGGCATCAGAGAGCTCT+GGG 0.519415 1_4:+50949692 None:intergenic
AGGTCTACTTTACGGAGGTA+AGG 0.526416 1_4:+50949733 None:intergenic
ATGAAAGGAAAGGAAGCCAT+TGG 0.526527 1_4:-50948563 Msa0168390:CDS
TTGGTTTGTGACAATAATAG+AGG 0.530133 1_4:+50951073 None:intergenic
ATGGTGGAGGTGGATTGATT+GGG 0.531331 1_4:-50947388 Msa0168390:three_prime_UTR
TGTGACAATAATAGAGGAAG+GGG 0.540700 1_4:+50951079 None:intergenic
ACAATTTAAGCCAAAGGAGA+AGG 0.543283 1_4:-50950796 Msa0168390:CDS
ACTGAACTGAGGCTAGGACT+TGG 0.544230 1_4:-50950942 Msa0168390:CDS
ATGTTTAACTCCTTTACCAC+AGG 0.544923 1_4:-50949664 Msa0168390:intron
AAGCTTTGTGAAAGTTAGCA+TGG 0.545131 1_4:-50949764 Msa0168390:CDS
GGTAACTCTTGTACATCTTG+AGG 0.548937 1_4:+50949713 None:intergenic
TTTGTGAAAGTTAGCATGGA+TGG 0.550924 1_4:-50949760 Msa0168390:CDS
ACGTATAGGGCACAAGTGGT+AGG 0.553577 1_4:-50949886 Msa0168390:intron
CTTCAGTTGCCAAGTCAACT+GGG 0.554821 1_4:+50947888 None:intergenic
CCAACTTATGAAGACAAAGA+TGG 0.567272 1_4:-50948902 Msa0168390:CDS
AACTGGGCATCACATGGAGC+AGG 0.567331 1_4:+50947904 None:intergenic
CGTATAGGGCACAAGTGGTA+GGG 0.568467 1_4:-50949885 Msa0168390:intron
AAGGCAAGAGCACGTTGAAG+TGG 0.578771 1_4:+50951108 None:intergenic
TGCTTGTCGGTGATGTACCA+TGG 0.581796 1_4:-50948871 Msa0168390:CDS
TATGTCAGCACCTAGAGCCA+TGG 0.584052 1_4:-50947975 Msa0168390:intron
TTGTGACAATAATAGAGGAA+GGG 0.584516 1_4:+50951078 None:intergenic
GCTGCCCAAAAGAGCAGTGA+AGG 0.587247 1_4:-50949826 Msa0168390:CDS
GCAAGAGCACGTTGAAGTGG+GGG 0.588919 1_4:+50951111 None:intergenic
ATGAAGACAAAGATGGCGAC+TGG 0.589440 1_4:-50948895 Msa0168390:CDS
TTCATAAGTTGGGACATAGT+CGG 0.596025 1_4:+50948913 None:intergenic
TTTACGTATCATGAAAGGAA+AGG 0.596184 1_4:-50948573 Msa0168390:CDS
AGATCCACAATTTAAGCCAA+AGG 0.598269 1_4:-50950802 Msa0168390:CDS
GGCTAGGACTTGGATTGCCA+GGG 0.598855 1_4:-50950932 Msa0168390:CDS
AAGCGTTTACGTATCATGAA+AGG 0.600017 1_4:-50948578 Msa0168390:CDS
GGCAAGAGCACGTTGAAGTG+GGG 0.601053 1_4:+50951110 None:intergenic
GAATTGAAAACTTACTCCCA+TGG 0.601391 1_4:+50948854 None:intergenic
GATGGCGACTGGATGCTTGT+CGG 0.601801 1_4:-50948884 Msa0168390:CDS
TTACGGAATGACCGCACTGG+TGG 0.603134 1_4:+50949859 None:intergenic
GCTTGTCGGTGATGTACCAT+GGG 0.605812 1_4:-50948870 Msa0168390:CDS
CAAGTCAACTGGGCATCACA+TGG 0.606051 1_4:+50947898 None:intergenic
GAGGAAACTGAACTGAGGCT+AGG 0.606535 1_4:-50950948 Msa0168390:CDS
TTCTTACGGAATGACCGCAC+TGG 0.606920 1_4:+50949856 None:intergenic
CTAGTCCCTAAACATGGTGG+AGG 0.607718 1_4:-50947401 Msa0168390:three_prime_UTR
CTGTACGTATAGGGCACAAG+TGG 0.611935 1_4:-50949890 Msa0168390:intron
ACAGAAGTAATAAGGAAGAG+AGG 0.613004 1_4:-50950903 Msa0168390:CDS
ATCATACTAACCTAGTCCAA+TGG 0.619665 1_4:+50948547 None:intergenic
GAAGGAAATTGTGAATCCCA+AGG 0.620096 1_4:-50948989 Msa0168390:intron
TGAACAACTTACTTAGCAGG+AGG 0.624213 1_4:+50950744 None:intergenic
TCTTATGCAAGCACACAACA+AGG 0.626643 1_4:+50947654 None:intergenic
ACTTGATAATGGAAGTTGCA+GGG 0.628469 1_4:-50950998 Msa0168390:exon
CAAGAGCACGTTGAAGTGGG+GGG 0.644816 1_4:+50951112 None:intergenic
ATGCTATTTGAAAATGCACA+TGG 0.668928 1_4:+50947353 None:intergenic
TGGTAGGGTGGCCACCAGTG+CGG 0.683033 1_4:-50949870 Msa0168390:CDS
ATAGGGCACAAGTGGTAGGG+TGG 0.690264 1_4:-50949882 Msa0168390:intron
ACGTAGCTAGTCCCTAAACA+TGG 0.698273 1_4:-50947407 Msa0168390:three_prime_UTR
TCTTGAGGTCTACTTTACGG+AGG 0.703203 1_4:+50949728 None:intergenic
TAGCTAGTCCCTAAACATGG+TGG 0.721127 1_4:-50947404 Msa0168390:three_prime_UTR
TGAAAGTGAAACAAACACTG+TGG 0.729821 1_4:-50950862 Msa0168390:CDS
TGTTTGAGGAAACTGAACTG+AGG 0.788719 1_4:-50950953 Msa0168390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAAATAAATAAAAAA+GGG + chr1_4:50949682-50949701 None:intergenic 0.0%
!! ATTAAAAAATAAATAAAATT+TGG - chr1_4:50949747-50949766 Msa0168390:CDS 0.0%
!! ATATAAATTATTAATCACAA+AGG + chr1_4:50947927-50947946 None:intergenic 10.0%
!! GATAACAATATTATATAATA+AGG + chr1_4:50948208-50948227 None:intergenic 10.0%
!!! TTTTAATTGTGAATTAAATA+TGG - chr1_4:50948255-50948274 Msa0168390:intron 10.0%
!!! GAACTATTTTAAAATTTATA+TGG + chr1_4:50948306-50948325 None:intergenic 10.0%
!!! TTAAAATAGTTCTATATTAA+AGG - chr1_4:50948314-50948333 Msa0168390:intron 10.0%
!! AAATTAAATTTATTCTTTCA+TGG + chr1_4:50949363-50949382 None:intergenic 10.0%
!!! TAAATTTAATTTCATAGAAA+TGG - chr1_4:50949371-50949390 Msa0168390:intron 10.0%
!!! AAATTTAATTTCATAGAAAT+GGG - chr1_4:50949372-50949391 Msa0168390:intron 10.0%
!!! AAGATATTTTTATAAGTAAA+GGG + chr1_4:50950420-50950439 None:intergenic 10.0%
!!! AAAGATATTTTTATAAGTAA+AGG + chr1_4:50950421-50950440 None:intergenic 10.0%
!! AAAATCATTAATTTAATACA+AGG + chr1_4:50950961-50950980 None:intergenic 10.0%
!!! TTATGTTCCAATTAATTTTA+TGG + chr1_4:50947871-50947890 None:intergenic 15.0%
!!! AAAATTTGCTATATTGTTAT+GGG + chr1_4:50948044-50948063 None:intergenic 15.0%
!! AAACTATCAACTTTATAATA+AGG - chr1_4:50948352-50948371 Msa0168390:intron 15.0%
!! AATTTCATTGCTAAAATAAA+TGG - chr1_4:50948446-50948465 Msa0168390:intron 15.0%
!! TATTATTAATATGTTAGATG+AGG - chr1_4:50948869-50948888 Msa0168390:CDS 15.0%
!! ATTATTAATATGTTAGATGA+GGG - chr1_4:50948870-50948889 Msa0168390:CDS 15.0%
!!! GCAAAATAGTTTTATAAAAA+GGG + chr1_4:50949017-50949036 None:intergenic 15.0%
!!! AGCAAAATAGTTTTATAAAA+AGG + chr1_4:50949018-50949037 None:intergenic 15.0%
!! TATACATACAATAAAATTTG+TGG + chr1_4:50949279-50949298 None:intergenic 15.0%
!! TTTATATTGAAATTGATTGA+AGG - chr1_4:50949475-50949494 Msa0168390:intron 15.0%
!!! TATTTAATTTGCATAGAAAT+TGG - chr1_4:50951020-50951039 Msa0168390:five_prime_UTR 15.0%
!!! TTCCAATTAATTTTATGGAT+CGG + chr1_4:50947866-50947885 None:intergenic 20.0%
!! AATTATTAATCACAAAGGAA+TGG + chr1_4:50947922-50947941 None:intergenic 20.0%
!!! AAATTTGCTATATTGTTATG+GGG + chr1_4:50948043-50948062 None:intergenic 20.0%
!!! CAAAATTTGCTATATTGTTA+TGG + chr1_4:50948045-50948064 None:intergenic 20.0%
!! TACTTATAGAAAAAAGTAAG+AGG + chr1_4:50948111-50948130 None:intergenic 20.0%
!! GTACAATAAAAACATATAGT+AGG + chr1_4:50948386-50948405 None:intergenic 20.0%
!! ATAGTTAAAAAAATTACCTG+TGG + chr1_4:50948837-50948856 None:intergenic 20.0%
!! TTATCAAAAAAAAATTACGC+AGG - chr1_4:50948940-50948959 Msa0168390:CDS 20.0%
!! TATCAAAAAAAAATTACGCA+GGG - chr1_4:50948941-50948960 Msa0168390:CDS 20.0%
!!! TATAATAAATAAGGGTATGT+AGG + chr1_4:50949069-50949088 None:intergenic 20.0%
!!! AAACAAACTATCTCTTTTAA+AGG - chr1_4:50949132-50949151 Msa0168390:intron 20.0%
!!! CCTTATTTATAAGACTTTTT+TGG + chr1_4:50949235-50949254 None:intergenic 20.0%
!!! CCAAAAAAGTCTTATAAATA+AGG - chr1_4:50949232-50949251 Msa0168390:intron 20.0%
!!! AAAAGTCTTATAAATAAGGA+TGG - chr1_4:50949236-50949255 Msa0168390:intron 20.0%
!! GCAATATTAAGACAAAATTA+AGG + chr1_4:50949320-50949339 None:intergenic 20.0%
!!! AATTTTGTCTTAATATTGCA+TGG - chr1_4:50949321-50949340 Msa0168390:intron 20.0%
!! AGATCAATATCAAGTATTAA+TGG - chr1_4:50949437-50949456 Msa0168390:intron 20.0%
!!! TTTTTGTTAGCTATATATTG+TGG + chr1_4:50949839-50949858 None:intergenic 20.0%
!! TTAATAGTATGCTCATAAAT+TGG - chr1_4:50950018-50950037 Msa0168390:intron 20.0%
!!! TTTTTTTTGAAGGATGTTTA+GGG + chr1_4:50950108-50950127 None:intergenic 20.0%
!!! TTTTTTTTTGAAGGATGTTT+AGG + chr1_4:50950109-50950128 None:intergenic 20.0%
!!! CTCTCTTTTTTTTTTTTTGA+AGG + chr1_4:50950118-50950137 None:intergenic 20.0%
!! CTTAAAGTAAAAGAAAGTTA+GGG + chr1_4:50950171-50950190 None:intergenic 20.0%
!! TCTTAAAGTAAAAGAAAGTT+AGG + chr1_4:50950172-50950191 None:intergenic 20.0%
!!! TTACTTTAAGATACAATAGA+TGG - chr1_4:50950181-50950200 Msa0168390:intron 20.0%
!! ACCTATATAACTCATTTATT+TGG - chr1_4:50950262-50950281 Msa0168390:intron 20.0%
!! ACCAAATAAATGAGTTATAT+AGG + chr1_4:50950266-50950285 None:intergenic 20.0%
!!! TTTGGTTCAAACAATTTAAT+TGG - chr1_4:50950280-50950299 Msa0168390:intron 20.0%
!! ATTCAAATGTTTAAAAGTTG+TGG + chr1_4:50950370-50950389 None:intergenic 20.0%
!! AAGAAAATTTAAAGACAACT+TGG + chr1_4:50950913-50950932 None:intergenic 20.0%
!!! TTGATCTTTTATTGTTAGTT+AGG - chr1_4:50951046-50951065 Msa0168390:five_prime_UTR 20.0%
! TTACGCATGCTTAAATATTT+AGG + chr1_4:50947768-50947787 None:intergenic 25.0%
! TTCCGATCCATAAAATTAAT+TGG - chr1_4:50947861-50947880 Msa0168390:three_prime_UTR 25.0%
!! TAGTTCTATATTAAAGGACA+AGG - chr1_4:50948320-50948339 Msa0168390:intron 25.0%
!! GAAAATTCTCAAGTTTTTGA+AGG + chr1_4:50948512-50948531 None:intergenic 25.0%
! AAAAAAATTACCTGTGGTAA+AGG + chr1_4:50948831-50948850 None:intergenic 25.0%
! ATCAAAAAAAAATTACGCAG+GGG - chr1_4:50948942-50948961 Msa0168390:CDS 25.0%
!!! AGACTTTTTTGGTAAAATCA+CGG + chr1_4:50949224-50949243 None:intergenic 25.0%
! TATGATTGAAATTAGTACGT+AGG + chr1_4:50949727-50949746 None:intergenic 25.0%
! AAATTGGTATTAAGTACAAG+AGG - chr1_4:50950034-50950053 Msa0168390:intron 25.0%
! TGACATTCTAAGCAAAATAA+CGG - chr1_4:50950468-50950487 Msa0168390:intron 25.0%
! TGTGTATGTGTAACATTATA+TGG - chr1_4:50950684-50950703 Msa0168390:intron 25.0%
! TTCTGGTCATAATTTATACT+TGG + chr1_4:50950756-50950775 None:intergenic 25.0%
TTGTGACAATAATAGAGGAA+GGG + chr1_4:50947407-50947426 None:intergenic 30.0%
TTTGTGACAATAATAGAGGA+AGG + chr1_4:50947408-50947427 None:intergenic 30.0%
!! TTGGTTTGTGACAATAATAG+AGG + chr1_4:50947412-50947431 None:intergenic 30.0%
GAGAGAGAAAAACTTGATAA+TGG - chr1_4:50947473-50947492 Msa0168390:three_prime_UTR 30.0%
GGAAAACAACAGAAGTAATA+AGG - chr1_4:50947571-50947590 Msa0168390:three_prime_UTR 30.0%
GAAGCTTAATCTTTCTACTA+AGG - chr1_4:50947647-50947666 Msa0168390:three_prime_UTR 30.0%
CTTAATCTTTCTACTAAGGA+AGG - chr1_4:50947651-50947670 Msa0168390:three_prime_UTR 30.0%
TAATGAACAACTTACTTAGC+AGG + chr1_4:50947744-50947763 None:intergenic 30.0%
ATAGAAAAAAGTAAGAGGCA+AGG + chr1_4:50948106-50948125 None:intergenic 30.0%
AGTTCATGATTGTATTGCTT+TGG + chr1_4:50948486-50948505 None:intergenic 30.0%
AAAGCAATACAATCATGAAC+TGG - chr1_4:50948485-50948504 Msa0168390:intron 30.0%
ACTAATTGAACTGTACGTAT+AGG - chr1_4:50948582-50948601 Msa0168390:CDS 30.0%
CTAATTGAACTGTACGTATA+GGG - chr1_4:50948583-50948602 Msa0168390:CDS 30.0%
CTAACTTTCACAAAGCTTAT+TGG + chr1_4:50948716-50948735 None:intergenic 30.0%
! GGTAAAGGAGTTAAACATTT+TGG + chr1_4:50948816-50948835 None:intergenic 30.0%
! AGTCTTATAATTAGAGACAG+AGG + chr1_4:50948978-50948997 None:intergenic 30.0%
!!! GACTTTTTTGGTAAAATCAC+GGG + chr1_4:50949223-50949242 None:intergenic 30.0%
! GAATGAGAGTAATAAGTTGA+TGG - chr1_4:50949531-50949550 Msa0168390:intron 30.0%
CATCTTTGTCTTCATAAGTT+GGG + chr1_4:50949582-50949601 None:intergenic 30.0%
CATTCTTCCACTGAATTAAT+TGG - chr1_4:50949650-50949669 Msa0168390:intron 30.0%
!! TTTATTTTCTTTGTGTCGAC+AGG - chr1_4:50949863-50949882 Msa0168390:CDS 30.0%
TTTACGTATCATGAAAGGAA+AGG - chr1_4:50949909-50949928 Msa0168390:intron 30.0%
!!! TCTTTGTTTTGGTGATTAAG+TGG + chr1_4:50950082-50950101 None:intergenic 30.0%
GGTTCAAACAATTTAATTGG+CGG - chr1_4:50950283-50950302 Msa0168390:intron 30.0%
TTGCTAAGAACAGAGAATAA+GGG - chr1_4:50950316-50950335 Msa0168390:intron 30.0%
! TTTAGAGTCATTATCGTTTC+TGG + chr1_4:50950773-50950792 None:intergenic 30.0%
TGTGACAATAATAGAGGAAG+GGG + chr1_4:50947406-50947425 None:intergenic 35.0%
AGCATTCAGTGTGATTTGAT+TGG + chr1_4:50947431-50947450 None:intergenic 35.0%
!! AACTTGATAATGGAAGTTGC+AGG - chr1_4:50947483-50947502 Msa0168390:three_prime_UTR 35.0%
!! ACTTGATAATGGAAGTTGCA+GGG - chr1_4:50947484-50947503 Msa0168390:three_prime_UTR 35.0%
!!! ATTACTTCTGTTGTTTTCCC+TGG + chr1_4:50947570-50947589 None:intergenic 35.0%
ACAGAAGTAATAAGGAAGAG+AGG - chr1_4:50947579-50947598 Msa0168390:three_prime_UTR 35.0%
TGAAAGTGAAACAAACACTG+TGG - chr1_4:50947620-50947639 Msa0168390:three_prime_UTR 35.0%
AGATCCACAATTTAAGCCAA+AGG - chr1_4:50947680-50947699 Msa0168390:three_prime_UTR 35.0%
! TTCTCCTTTGGCTTAAATTG+TGG + chr1_4:50947687-50947706 None:intergenic 35.0%
ACAATTTAAGCCAAAGGAGA+AGG - chr1_4:50947686-50947705 Msa0168390:three_prime_UTR 35.0%
GAGTACACATCAAGACTAAT+TGG + chr1_4:50948019-50948038 None:intergenic 35.0%
! GAAGTGCTTGAGTATATACA+TGG + chr1_4:50948562-50948581 None:intergenic 35.0%
! AAGCTTTGTGAAAGTTAGCA+TGG - chr1_4:50948718-50948737 Msa0168390:intron 35.0%
TTTGTGAAAGTTAGCATGGA+TGG - chr1_4:50948722-50948741 Msa0168390:intron 35.0%
ACATCTTGAGGTCTACTTTA+CGG + chr1_4:50948760-50948779 None:intergenic 35.0%
!!! GGAGTTAAACATTTTGGCTA+AGG + chr1_4:50948810-50948829 None:intergenic 35.0%
ATGTTTAACTCCTTTACCAC+AGG - chr1_4:50948818-50948837 Msa0168390:intron 35.0%
AGTGAAAGAGCGAAATGATA+AGG + chr1_4:50948912-50948931 None:intergenic 35.0%
CATGAAATCCTTAATGCCTT+GGG + chr1_4:50949512-50949531 None:intergenic 35.0%
TCATGAAATCCTTAATGCCT+TGG + chr1_4:50949513-50949532 None:intergenic 35.0%
! TTCATAAGTTGGGACATAGT+CGG + chr1_4:50949572-50949591 None:intergenic 35.0%
CCATCTTTGTCTTCATAAGT+TGG + chr1_4:50949583-50949602 None:intergenic 35.0%
CCAACTTATGAAGACAAAGA+TGG - chr1_4:50949580-50949599 Msa0168390:intron 35.0%
GAATTGAAAACTTACTCCCA+TGG + chr1_4:50949631-50949650 None:intergenic 35.0%
GTCACTACCAATTAATTCAG+TGG + chr1_4:50949660-50949679 None:intergenic 35.0%
TTAGCTATATATTGTGGTCG+TGG + chr1_4:50949833-50949852 None:intergenic 35.0%
AAGCGTTTACGTATCATGAA+AGG - chr1_4:50949904-50949923 Msa0168390:intron 35.0%
ATCATACTAACCTAGTCCAA+TGG + chr1_4:50949938-50949957 None:intergenic 35.0%
!! GTTTAGGGATCTCTTTGTTT+TGG + chr1_4:50950093-50950112 None:intergenic 35.0%
CTTGCTAAGAACAGAGAATA+AGG - chr1_4:50950315-50950334 Msa0168390:intron 35.0%
TGCTAAGAACAGAGAATAAG+GGG - chr1_4:50950317-50950336 Msa0168390:intron 35.0%
! ATAGAGGAAGGGGTATAGAA+AGG + chr1_4:50947396-50947415 None:intergenic 40.0%
GCTGAAAAAGGAGGAAAAGA+TGG - chr1_4:50947506-50947525 Msa0168390:three_prime_UTR 40.0%
TGTTTGAGGAAACTGAACTG+AGG - chr1_4:50947529-50947548 Msa0168390:three_prime_UTR 40.0%
TGAACAACTTACTTAGCAGG+AGG + chr1_4:50947741-50947760 None:intergenic 40.0%
ATCACAAAGGAATGGAACAC+AGG + chr1_4:50947914-50947933 None:intergenic 40.0%
! TGATCCTTCACTGCTCTTTT+GGG + chr1_4:50948663-50948682 None:intergenic 40.0%
GGTAACTCTTGTACATCTTG+AGG + chr1_4:50948772-50948791 None:intergenic 40.0%
TTAGAGACAGAGGTAGTAGT+TGG + chr1_4:50948968-50948987 None:intergenic 40.0%
CGTGCATGTGAATATACACA+TGG - chr1_4:50949413-50949432 Msa0168390:intron 40.0%
GAAGGAAATTGTGAATCCCA+AGG - chr1_4:50949493-50949512 Msa0168390:intron 40.0%
! TTGTGAATCCCAAGGCATTA+AGG - chr1_4:50949501-50949520 Msa0168390:intron 40.0%
GTGGTTTCTCTGTCTCATTA+TGG + chr1_4:50949814-50949833 None:intergenic 40.0%
ATGAAAGGAAAGGAAGCCAT+TGG - chr1_4:50949919-50949938 Msa0168390:intron 40.0%
! TTGCATTTTTCCATGGCTCT+AGG + chr1_4:50950520-50950539 None:intergenic 40.0%
! CCTGTTCTTGCATTTTTCCA+TGG + chr1_4:50950527-50950546 None:intergenic 40.0%
CCATGGAAAAATGCAAGAAC+AGG - chr1_4:50950524-50950543 Msa0168390:intron 40.0%
TAAGCTGCAGCAGAAACATA+TGG - chr1_4:50950623-50950642 Msa0168390:intron 40.0%
TCTTATGCAAGCACACAACA+AGG + chr1_4:50950831-50950850 None:intergenic 40.0%
GGAGGAAAAGATGGTGTTTG+AGG - chr1_4:50947515-50947534 Msa0168390:three_prime_UTR 45.0%
GGGCAGCAAACATGTTCTTA+CGG + chr1_4:50948643-50948662 None:intergenic 45.0%
! CTGATCCTTCACTGCTCTTT+TGG + chr1_4:50948664-50948683 None:intergenic 45.0%
AGGTCTACTTTACGGAGGTA+AGG + chr1_4:50948752-50948771 None:intergenic 45.0%
TCTTGAGGTCTACTTTACGG+AGG + chr1_4:50948757-50948776 None:intergenic 45.0%
ATGAAGACAAAGATGGCGAC+TGG - chr1_4:50949587-50949606 Msa0168390:intron 45.0%
CTTCAGTTGCCAAGTCAACT+GGG + chr1_4:50950597-50950616 None:intergenic 45.0%
ACGTAGCTAGTCCCTAAACA+TGG - chr1_4:50951075-50951094 Msa0168390:five_prime_UTR 45.0%
TAGCTAGTCCCTAAACATGG+TGG - chr1_4:50951078-50951097 Msa0168390:five_prime_UTR 45.0%
ATCCACCTCCACCATGTTTA+GGG + chr1_4:50951089-50951108 None:intergenic 45.0%
AATCCACCTCCACCATGTTT+AGG + chr1_4:50951090-50951109 None:intergenic 45.0%
!! ATGGTGGAGGTGGATTGATT+GGG - chr1_4:50951094-50951113 Msa0168390:five_prime_UTR 45.0%
!! AAAAAATAAAAAAAATAGTA+AGG + chr1_4:50948135-50948154 None:intergenic 5.0%
!!! TACTATTTTTTTTATTTTTT+TGG - chr1_4:50948135-50948154 Msa0168390:intron 5.0%
!! AAAAAAAATAGTATATAATA+AGG + chr1_4:50948167-50948186 None:intergenic 5.0%
!! AAAAAATGTATAATAAATAA+GGG + chr1_4:50949077-50949096 None:intergenic 5.0%
!! AAAAAAATGTATAATAAATA+AGG + chr1_4:50949078-50949097 None:intergenic 5.0%
!! CTAAAATAAATAAATAAAAA+AGG + chr1_4:50949683-50949702 None:intergenic 5.0%
! AGGCAAGAGCACGTTGAAGT+GGG + chr1_4:50947376-50947395 None:intergenic 50.0%
! AAGGCAAGAGCACGTTGAAG+TGG + chr1_4:50947377-50947396 None:intergenic 50.0%
GGAAGTTGCAGGGCTGAAAA+AGG - chr1_4:50947494-50947513 Msa0168390:three_prime_UTR 50.0%
AGTTGCAGGGCTGAAAAAGG+AGG - chr1_4:50947497-50947516 Msa0168390:three_prime_UTR 50.0%
GAGGAAACTGAACTGAGGCT+AGG - chr1_4:50947534-50947553 Msa0168390:three_prime_UTR 50.0%
ACTGAACTGAGGCTAGGACT+TGG - chr1_4:50947540-50947559 Msa0168390:three_prime_UTR 50.0%
AGCAGAAGAGCCTTCTCCTT+TGG + chr1_4:50947699-50947718 None:intergenic 50.0%
GCTCTTCTGCTCTCTGATTC+TGG - chr1_4:50947708-50947727 Msa0168390:three_prime_UTR 50.0%
CTGTACGTATAGGGCACAAG+TGG - chr1_4:50948592-50948611 Msa0168390:CDS 50.0%
ACGTATAGGGCACAAGTGGT+AGG - chr1_4:50948596-50948615 Msa0168390:CDS 50.0%
CGTATAGGGCACAAGTGGTA+GGG - chr1_4:50948597-50948616 Msa0168390:CDS 50.0%
TTCTTACGGAATGACCGCAC+TGG + chr1_4:50948629-50948648 None:intergenic 50.0%
! CTAAGGCATCAGAGAGCTCT+GGG + chr1_4:50948793-50948812 None:intergenic 50.0%
TGCTTGTCGGTGATGTACCA+TGG - chr1_4:50949611-50949630 Msa0168390:intron 50.0%
GCTTGTCGGTGATGTACCAT+GGG - chr1_4:50949612-50949631 Msa0168390:intron 50.0%
GGAAAGGAAGCCATTGGACT+AGG - chr1_4:50949925-50949944 Msa0168390:intron 50.0%
TATGTCAGCACCTAGAGCCA+TGG - chr1_4:50950507-50950526 Msa0168390:intron 50.0%
GCATCACATGGAGCAGGTTA+TGG + chr1_4:50950575-50950594 None:intergenic 50.0%
CAAGTCAACTGGGCATCACA+TGG + chr1_4:50950587-50950606 None:intergenic 50.0%
CATGTGATGCCCAGTTGACT+TGG - chr1_4:50950585-50950604 Msa0168390:intron 50.0%
GCTTCAGTTGCCAAGTCAAC+TGG + chr1_4:50950598-50950617 None:intergenic 50.0%
CTAGTCCCTAAACATGGTGG+AGG - chr1_4:50951081-50951100 Msa0168390:five_prime_UTR 50.0%
!! CATGGTGGAGGTGGATTGAT+TGG - chr1_4:50951093-50951112 Msa0168390:five_prime_UTR 50.0%
!! TGGAGGTGGATTGATTGGGT+GGG - chr1_4:50951098-50951117 Msa0168390:five_prime_UTR 50.0%
! CAAGAGCACGTTGAAGTGGG+GGG + chr1_4:50947373-50947392 None:intergenic 55.0%
! GCAAGAGCACGTTGAAGTGG+GGG + chr1_4:50947374-50947393 None:intergenic 55.0%
! GGCAAGAGCACGTTGAAGTG+GGG + chr1_4:50947375-50947394 None:intergenic 55.0%
AGGCTAGGACTTGGATTGCC+AGG - chr1_4:50947549-50947568 Msa0168390:three_prime_UTR 55.0%
GGCTAGGACTTGGATTGCCA+GGG - chr1_4:50947550-50947569 Msa0168390:three_prime_UTR 55.0%
ATAGGGCACAAGTGGTAGGG+TGG - chr1_4:50948600-50948619 Msa0168390:CDS 55.0%
TTACGGAATGACCGCACTGG+TGG + chr1_4:50948626-50948645 None:intergenic 55.0%
GCTGCCCAAAAGAGCAGTGA+AGG - chr1_4:50948656-50948675 Msa0168390:intron 55.0%
! GCTAAGGCATCAGAGAGCTC+TGG + chr1_4:50948794-50948813 None:intergenic 55.0%
GATGGCGACTGGATGCTTGT+CGG - chr1_4:50949598-50949617 Msa0168390:intron 55.0%
AACTGGGCATCACATGGAGC+AGG + chr1_4:50950581-50950600 None:intergenic 55.0%
! GTCCCTAAACATGGTGGAGG+TGG - chr1_4:50951084-50951103 Msa0168390:five_prime_UTR 55.0%
!! GTGGAGGTGGATTGATTGGG+TGG - chr1_4:50951097-50951116 Msa0168390:five_prime_UTR 55.0%
!! GGAGGTGGATTGATTGGGTG+GGG - chr1_4:50951099-50951118 Msa0168390:five_prime_UTR 55.0%
!! GAGGTGGATTGATTGGGTGG+GGG - chr1_4:50951100-50951119 Msa0168390:five_prime_UTR 55.0%
TGGTAGGGTGGCCACCAGTG+CGG - chr1_4:50948612-50948631 Msa0168390:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 50947355 50951149 50947355 ID=Msa0168390;Name=Msa0168390
chr1_4 mRNA 50947355 50951149 50947355 ID=Msa0168390-mRNA-1;Parent=Msa0168390;Name=Msa0168390-mRNA-1;_AED=0.04;_eAED=0.04;_QI=137|1|1|1|1|1|5|597|243
chr1_4 exon 50947355 50947989 50947355 ID=Msa0168390-mRNA-1:exon:14991;Parent=Msa0168390-mRNA-1
chr1_4 exon 50948558 50948619 50948558 ID=Msa0168390-mRNA-1:exon:14990;Parent=Msa0168390-mRNA-1
chr1_4 exon 50948869 50949007 50948869 ID=Msa0168390-mRNA-1:exon:14989;Parent=Msa0168390-mRNA-1
chr1_4 exon 50949665 50949900 50949665 ID=Msa0168390-mRNA-1:exon:14988;Parent=Msa0168390-mRNA-1
chr1_4 exon 50950756 50951149 50950756 ID=Msa0168390-mRNA-1:exon:14987;Parent=Msa0168390-mRNA-1
chr1_4 five_prime_UTR 50951013 50951149 50951013 ID=Msa0168390-mRNA-1:five_prime_utr;Parent=Msa0168390-mRNA-1
chr1_4 CDS 50950756 50951012 50950756 ID=Msa0168390-mRNA-1:cds;Parent=Msa0168390-mRNA-1
chr1_4 CDS 50949665 50949900 50949665 ID=Msa0168390-mRNA-1:cds;Parent=Msa0168390-mRNA-1
chr1_4 CDS 50948869 50949007 50948869 ID=Msa0168390-mRNA-1:cds;Parent=Msa0168390-mRNA-1
chr1_4 CDS 50948558 50948619 50948558 ID=Msa0168390-mRNA-1:cds;Parent=Msa0168390-mRNA-1
chr1_4 CDS 50947952 50947989 50947952 ID=Msa0168390-mRNA-1:cds;Parent=Msa0168390-mRNA-1
chr1_4 three_prime_UTR 50947355 50947951 50947355 ID=Msa0168390-mRNA-1:three_prime_utr;Parent=Msa0168390-mRNA-1
Gene Sequence

>Msa0168390

ATGGAAGTTGCAGGGCTGAAAAAGGAGGAAAAGATGGTGTTTGAGGAAACTGAACTGAGGCTAGGACTTGGATTGCCAGGGAAAACAACAGAAGTAATAAGGAAGAGAGGTTTTTCTGAAACTGAGAGTGAAAGTGAAACAAACACTGTGGATTTGAAGCTTAATCTTTCTACTAAGGAAGGAGCAACAGATCCACAATTTAAGCCAAAGGAGAAGGCTCTTCTGCTCTCTGATTCTGGTGTCAAGCCTCCTGCTAAGGCACAAGTGGTAGGGTGGCCACCAGTGCGGTCATTCCGTAAGAACATGTTTGCTGCCCAAAAGAGCAGTGAAGGATCAGAAGAAAGTGAAAAGAAGAATAGCACTAATCCAATAAGCTTTGTGAAAGTTAGCATGGATGGAGCACCTTACCTCCGTAAAGTAGACCTCAAGATGTACAAGAGTTACCCAGAGCTCTCTGATGCCTTAGCCAAAATGTTTAACTCCTTTACCACAGGAAATTGTGAATCCCAAGGCATTAAGGATTTCATGAATGAGAGTAATAAGTTGATGGATCTATTGAACAGCTCCGACTATGTCCCAACTTATGAAGACAAAGATGGCGACTGGATGCTTGTCGGTGATGTACCATGGGAGATGTTTATTGATTCATGCAAGCGTTTACGTATCATGAAAGGAAAGGAAGCCATTGGACTAGCACCTAGAGCCATGGAAAAATGCAAGAACAGGAGCTAG
Protein sequence

>Msa0168390

MEVAGLKKEEKMVFEETELRLGLGLPGKTTEVIRKRGFSETESESETNTVDLKLNLSTKEGATDPQFKPKEKALLLSDSGVKPPAKAQVVGWPPVRSFRKNMFAAQKSSEGSEESEKKNSTNPISFVKVSMDGAPYLRKVDLKMYKSYPELSDALAKMFNSFTTGNCESQGIKDFMNESNKLMDLLNSSDYVPTYEDKDGDWMLVGDVPWEMFIDSCKRLRIMKGKEAIGLAPRAMEKCKNRS*