Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009311.t1 | AES68336.2 | 94.9 | 352 | 18 | 0 | 1 | 352 | 1 | 352 | 5.30E-194 | 686.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009311.t1 | G7J093 | 94.9 | 352 | 18 | 0 | 1 | 352 | 1 | 352 | 3.8e-194 | 686.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009311.t1 | MTR_3g006120 | 94.886 | 352 | 18 | 0 | 1 | 352 | 1 | 352 | 0.0 | 691 |
| MS.gene009311.t1 | MTR_3g006100 | 92.264 | 349 | 27 | 0 | 1 | 349 | 1 | 349 | 0.0 | 670 |
| MS.gene009311.t1 | MTR_3g006180 | 92.604 | 338 | 25 | 0 | 14 | 351 | 1 | 338 | 0.0 | 649 |
| MS.gene009311.t1 | MTR_3g006010 | 86.932 | 352 | 46 | 0 | 1 | 352 | 118 | 469 | 0.0 | 639 |
| MS.gene009311.t1 | MTR_3g006140 | 73.837 | 344 | 90 | 0 | 4 | 347 | 26 | 369 | 0.0 | 533 |
| MS.gene009311.t1 | MTR_4g133490 | 70.520 | 346 | 90 | 2 | 9 | 349 | 7 | 345 | 2.55e-168 | 473 |
| MS.gene009311.t1 | MTR_4g133320 | 70.684 | 307 | 86 | 2 | 44 | 349 | 2 | 305 | 9.31e-146 | 414 |
| MS.gene009311.t1 | MTR_3g068220 | 58.611 | 360 | 132 | 4 | 1 | 349 | 1 | 354 | 1.08e-138 | 398 |
| MS.gene009311.t1 | MTR_6g091830 | 55.866 | 358 | 145 | 5 | 4 | 352 | 102 | 455 | 2.87e-135 | 393 |
| MS.gene009311.t1 | MTR_6g092140 | 55.587 | 358 | 146 | 5 | 4 | 352 | 102 | 455 | 5.25e-134 | 390 |
| MS.gene009311.t1 | MTR_7g070100 | 58.092 | 346 | 141 | 4 | 8 | 352 | 8 | 350 | 7.81e-129 | 373 |
| MS.gene009311.t1 | MTR_3g045700 | 55.899 | 356 | 147 | 5 | 1 | 352 | 1 | 350 | 1.73e-127 | 369 |
| MS.gene009311.t1 | MTR_7g083950 | 55.028 | 358 | 146 | 8 | 1 | 352 | 84 | 432 | 7.52e-119 | 350 |
| MS.gene009311.t1 | MTR_6g088340 | 55.732 | 314 | 126 | 5 | 48 | 352 | 124 | 433 | 1.00e-109 | 327 |
| MS.gene009311.t1 | MTR_1g100280 | 50.000 | 358 | 137 | 7 | 4 | 352 | 13 | 337 | 5.51e-105 | 311 |
| MS.gene009311.t1 | MTR_6g011110 | 56.272 | 279 | 110 | 3 | 83 | 352 | 2 | 277 | 4.57e-100 | 297 |
| MS.gene009311.t1 | MTR_1g109340 | 60.079 | 253 | 93 | 3 | 103 | 351 | 106 | 354 | 1.45e-94 | 286 |
| MS.gene009311.t1 | MTR_3g085910 | 47.664 | 321 | 109 | 6 | 6 | 316 | 5 | 276 | 1.18e-91 | 275 |
| MS.gene009311.t1 | MTR_3g086480 | 56.641 | 256 | 105 | 4 | 90 | 344 | 3 | 253 | 6.18e-86 | 260 |
| MS.gene009311.t1 | MTR_1g109520 | 59.494 | 158 | 60 | 1 | 198 | 351 | 19 | 176 | 3.20e-57 | 184 |
| MS.gene009311.t1 | MTR_1g089620 | 60.448 | 134 | 50 | 1 | 175 | 308 | 30 | 160 | 3.42e-47 | 162 |
| MS.gene009311.t1 | MTR_1g012330 | 63.504 | 137 | 50 | 0 | 83 | 219 | 2 | 138 | 1.98e-45 | 156 |
| MS.gene009311.t1 | MTR_1g089085 | 57.447 | 141 | 55 | 3 | 4 | 143 | 39 | 175 | 2.49e-45 | 153 |
| MS.gene009311.t1 | MTR_1g109090 | 50.388 | 129 | 63 | 1 | 1 | 128 | 13 | 141 | 3.62e-35 | 126 |
| MS.gene009311.t1 | MTR_1g106050 | 58.879 | 107 | 42 | 1 | 4 | 108 | 2 | 108 | 1.38e-34 | 124 |
| MS.gene009311.t1 | MTR_6g023220 | 27.759 | 299 | 208 | 6 | 9 | 305 | 18 | 310 | 4.33e-29 | 115 |
| MS.gene009311.t1 | MTR_1g093300 | 52.336 | 107 | 51 | 0 | 176 | 282 | 1 | 107 | 1.76e-27 | 105 |
| MS.gene009311.t1 | MTR_0128s0040 | 42.105 | 133 | 75 | 2 | 33 | 165 | 3 | 133 | 3.88e-25 | 99.4 |
| MS.gene009311.t1 | MTR_6g091850 | 43.564 | 101 | 51 | 2 | 258 | 352 | 1 | 101 | 3.89e-24 | 95.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCTAAAAATTTTATATCTG+TGG | + | 24081:24925-24944 | MS.gene009311:intergenic | 15.0% |
| !!! | ATAAGAAGAATTGACTTTAA+AGG | + | 24081:24676-24695 | MS.gene009311:intergenic | 20.0% |
| ! | AACTTATTGGAAAAAGTTCT+TGG | - | 24081:24425-24444 | MS.gene009311:CDS | 25.0% |
| ! | AAGATGGTTGTAAAATTTCT+TGG | - | 24081:24313-24332 | MS.gene009311:CDS | 25.0% |
| ! | AGATGGTTGTAAAATTTCTT+GGG | - | 24081:24314-24333 | MS.gene009311:CDS | 25.0% |
| ! | GGGAATTTCATAAAACTTAT+TGG | - | 24081:24412-24431 | MS.gene009311:CDS | 25.0% |
| ! | GTAAACATTCAATATTTCCT+CGG | + | 24081:25033-25052 | MS.gene009311:intergenic | 25.0% |
| ! | TTTGAATAAGTAATGGTTCT+TGG | + | 24081:24455-24474 | MS.gene009311:intergenic | 25.0% |
| !! | CTATCATGCTTAATGTATTT+TGG | + | 24081:25248-25267 | MS.gene009311:intergenic | 25.0% |
| !! | GAAGACTTTTGAATAAGTAA+TGG | + | 24081:24462-24481 | MS.gene009311:intergenic | 25.0% |
| !!! | ATTGCATTATTTTATGCTTC+GGG | - | 24081:25104-25123 | MS.gene009311:CDS | 25.0% |
| !!! | CATTGCATTATTTTATGCTT+CGG | - | 24081:25103-25122 | MS.gene009311:CDS | 25.0% |
| AACATGTAAAGTGTGTTTAG+GGG | - | 24081:25208-25227 | MS.gene009311:CDS | 30.0% | |
| ACAACATGTAAAGTGTGTTT+AGG | - | 24081:25206-25225 | MS.gene009311:CDS | 30.0% | |
| ACCTTCTTTCTTCTTGTAAA+TGG | + | 24081:24639-24658 | MS.gene009311:intergenic | 30.0% | |
| CAACATGTAAAGTGTGTTTA+GGG | - | 24081:25207-25226 | MS.gene009311:CDS | 30.0% | |
| GGAAAAGGATTTCTTATCAT+TGG | + | 24081:24484-24503 | MS.gene009311:intergenic | 30.0% | |
| TAAACACACTTTACATGTTG+TGG | + | 24081:25207-25226 | MS.gene009311:intergenic | 30.0% | |
| TCCATTTACAAGAAGAAAGA+AGG | - | 24081:24635-24654 | MS.gene009311:CDS | 30.0% | |
| TTCCATCTTGACAAAATTCA+TGG | + | 24081:25072-25091 | MS.gene009311:intergenic | 30.0% | |
| TTTGTCAAGATGGAAACTTT+GGG | - | 24081:25077-25096 | MS.gene009311:CDS | 30.0% | |
| ! | AACCATGAATTTTGTCAAGA+TGG | - | 24081:25067-25086 | MS.gene009311:CDS | 30.0% |
| ! | TTTTGTCAAGATGGAAACTT+TGG | - | 24081:25076-25095 | MS.gene009311:CDS | 30.0% |
| !! | TTGCATTATTTTATGCTTCG+GGG | - | 24081:25105-25124 | MS.gene009311:CDS | 30.0% |
| AGTTTGTATGTCTATCAGTC+TGG | - | 24081:24774-24793 | MS.gene009311:CDS | 35.0% | |
| ATTCAACAATGGAAAGTCCA+TGG | - | 24081:24817-24836 | MS.gene009311:CDS | 35.0% | |
| CTGGGTTACATATTCAACAA+TGG | - | 24081:24806-24825 | MS.gene009311:CDS | 35.0% | |
| CTTTAGACATCTACACTATG+AGG | - | 24081:25313-25332 | MS.gene009311:CDS | 35.0% | |
| GTCTATCAGTCTGGAAATTT+GGG | - | 24081:24783-24802 | MS.gene009311:CDS | 35.0% | |
| TGTCTATCAGTCTGGAAATT+TGG | - | 24081:24782-24801 | MS.gene009311:CDS | 35.0% | |
| TGTTGTGGATGAAAGATTGA+TGG | + | 24081:25192-25211 | MS.gene009311:intergenic | 35.0% | |
| ! | CCTTGGAGTGATAAACTTTT+CGG | + | 24081:24524-24543 | MS.gene009311:intergenic | 35.0% |
| !! | TGCATTATTTTATGCTTCGG+GGG | - | 24081:25106-25125 | MS.gene009311:CDS | 35.0% |
| AACAATGGAAAGTCCATGGA+AGG | - | 24081:24821-24840 | MS.gene009311:CDS | 40.0% | |
| ACAAAGTCAACAACCTTCCA+TGG | + | 24081:24837-24856 | MS.gene009311:intergenic | 40.0% | |
| AGCGGATATTTGATCATGAC+AGG | - | 24081:24585-24604 | MS.gene009311:CDS | 40.0% | |
| AGCTATGCTGATGAAGGAAA+AGG | + | 24081:24499-24518 | MS.gene009311:intergenic | 40.0% | |
| AGTGAGGTAGATGTTACATC+GGG | + | 24081:24960-24979 | MS.gene009311:intergenic | 40.0% | |
| AGTGATTCATATTGCTTCCG+AGG | - | 24081:25013-25032 | MS.gene009311:CDS | 40.0% | |
| CAAGTCAAGTACACCTATGT+TGG | + | 24081:24898-24917 | MS.gene009311:intergenic | 40.0% | |
| CCGAAAAGTTTATCACTCCA+AGG | - | 24081:24521-24540 | MS.gene009311:CDS | 40.0% | |
| CGGATATTTGATCATGACAG+GGG | - | 24081:24587-24606 | MS.gene009311:CDS | 40.0% | |
| GAGTGAGGTAGATGTTACAT+CGG | + | 24081:24961-24980 | MS.gene009311:intergenic | 40.0% | |
| GCGGATATTTGATCATGACA+GGG | - | 24081:24586-24605 | MS.gene009311:CDS | 40.0% | |
| GTCATCGAAATCTAGATCTC+TGG | + | 24081:24358-24377 | MS.gene009311:intergenic | 40.0% | |
| GTCGCTTCATGTAAAAGTTC+TGG | - | 24081:24750-24769 | MS.gene009311:CDS | 40.0% | |
| TAGACATCTACACTATGAGG+TGG | - | 24081:25316-25335 | MS.gene009311:CDS | 40.0% | |
| TATATACGTTGTCACCGACA+AGG | - | 24081:24872-24891 | MS.gene009311:CDS | 40.0% | |
| ! | TACCATGTCTTGCTTGCTTT+TGG | - | 24081:24702-24721 | MS.gene009311:CDS | 40.0% |
| AAAGGAAAACGCCTACTTGC+TGG | - | 24081:25283-25302 | MS.gene009311:CDS | 45.0% | |
| ACACACCAGCGAATTGAAAG+AGG | + | 24081:24380-24399 | MS.gene009311:intergenic | 45.0% | |
| ATCAGTCTGGAAATTTGGGC+TGG | - | 24081:24787-24806 | MS.gene009311:CDS | 45.0% | |
| CATCACAACTAACCATCCTG+AGG | + | 24081:24983-25002 | MS.gene009311:intergenic | 45.0% | |
| CCAAGGCGATCAATCAATTC+TGG | - | 24081:24538-24557 | MS.gene009311:CDS | 45.0% | |
| CCAGAATTGATTGATCGCCT+TGG | + | 24081:24541-24560 | MS.gene009311:intergenic | 45.0% | |
| CTTTCAAAACCCCACCTTCT+CGG | + | 24081:25155-25174 | MS.gene009311:intergenic | 45.0% | |
| GATGACCTCTTTCAATTCGC+TGG | - | 24081:24372-24391 | MS.gene009311:CDS | 45.0% | |
| TAACATCTACCTCACTCCTC+AGG | - | 24081:24964-24983 | MS.gene009311:CDS | 45.0% | |
| TAGAACTCGAGTTCCTCCAA+AGG | - | 24081:25265-25284 | MS.gene009311:CDS | 45.0% | |
| TCAGTCTGGAAATTTGGGCT+GGG | - | 24081:24788-24807 | MS.gene009311:CDS | 45.0% | |
| TGCCAAAAGCAAGCAAGACA+TGG | + | 24081:24707-24726 | MS.gene009311:intergenic | 45.0% | |
| TGGTGTGTGCAAGAATTGGA+GGG | - | 24081:24392-24411 | MS.gene009311:CDS | 45.0% | |
| TTCTGGCACGTTGCTTATTC+TGG | - | 24081:24555-24574 | MS.gene009311:CDS | 45.0% | |
| TTTCAAAACCCCACCTTCTC+GGG | + | 24081:25154-25173 | MS.gene009311:intergenic | 45.0% | |
| ! | AAAACGCCTACTTGCTGGAT+TGG | - | 24081:25288-25307 | MS.gene009311:CDS | 45.0% |
| ! | AAAGCACCAATCCAGCAAGT+AGG | + | 24081:25297-25316 | MS.gene009311:intergenic | 45.0% |
| ! | AAGTAGGCGTTTTCCTTTGG+AGG | + | 24081:25281-25300 | MS.gene009311:intergenic | 45.0% |
| ! | AGCAAGTAGGCGTTTTCCTT+TGG | + | 24081:25284-25303 | MS.gene009311:intergenic | 45.0% |
| ! | CTCAGGATGGTTAGTTGTGA+TGG | - | 24081:24981-25000 | MS.gene009311:CDS | 45.0% |
| ! | GCTTATTCTGGCTGTTCTAG+CGG | - | 24081:24567-24586 | MS.gene009311:CDS | 45.0% |
| !! | GTCTTGCTTGCTTTTGGCAA+AGG | - | 24081:24708-24727 | MS.gene009311:CDS | 45.0% |
| !! | TACACCTATGTTGGCCTTGT+CGG | + | 24081:24889-24908 | MS.gene009311:intergenic | 45.0% |
| ACTAACCATCCTGAGGAGTG+AGG | + | 24081:24976-24995 | MS.gene009311:intergenic | 50.0% | |
| ATCTACCTCACTCCTCAGGA+TGG | - | 24081:24968-24987 | MS.gene009311:CDS | 50.0% | |
| ATGCATTGAGCAACCCGAGA+AGG | - | 24081:25138-25157 | MS.gene009311:CDS | 50.0% | |
| ATTGAGCAACCCGAGAAGGT+GGG | - | 24081:25142-25161 | MS.gene009311:CDS | 50.0% | |
| CTGGTGTGTGCAAGAATTGG+AGG | - | 24081:24391-24410 | MS.gene009311:CDS | 50.0% | |
| TCGCTGGTGTGTGCAAGAAT+TGG | - | 24081:24388-24407 | MS.gene009311:CDS | 50.0% | |
| TCGGTCAGCTATGCTGATGA+AGG | + | 24081:24505-24524 | MS.gene009311:intergenic | 50.0% | |
| ! | TGCTTTTGGCAAAGGCTCAG+AGG | - | 24081:24716-24735 | MS.gene009311:CDS | 50.0% |
| CATTGAGCAACCCGAGAAGG+TGG | - | 24081:25141-25160 | MS.gene009311:CDS | 55.0% | |
| GTCACCGACAAGGCCAACAT+AGG | - | 24081:24882-24901 | MS.gene009311:CDS | 55.0% | |
| TTGAGCAACCCGAGAAGGTG+GGG | - | 24081:25143-25162 | MS.gene009311:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24081 | gene | 24300 | 25358 | 24300 | ID=MS.gene009311 |
| 24081 | mRNA | 24300 | 25358 | 24300 | ID=MS.gene009311.t1;Parent=MS.gene009311 |
| 24081 | exon | 24300 | 25358 | 24300 | ID=MS.gene009311.t1.exon1;Parent=MS.gene009311.t1 |
| 24081 | CDS | 24300 | 25358 | 24300 | ID=cds.MS.gene009311.t1;Parent=MS.gene009311.t1 |
| Gene Sequence |
| Protein sequence |
