AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05311

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05311.t1 MTR_6g091830 82.430 461 60 5 1 446 1 455 0.0 764
MS.gene05311.t1 MTR_6g092140 81.996 461 62 5 1 446 1 455 0.0 760
MS.gene05311.t1 MTR_6g088340 74.304 467 65 5 1 446 1 433 0.0 679
MS.gene05311.t1 MTR_3g068220 68.362 354 100 1 103 444 2 355 2.38e-173 490
MS.gene05311.t1 MTR_3g006140 56.131 367 138 4 94 445 15 373 7.57e-136 395
MS.gene05311.t1 MTR_4g133490 57.423 357 127 5 105 446 2 348 1.65e-135 393
MS.gene05311.t1 MTR_3g006010 46.414 474 202 9 6 443 9 466 2.14e-135 397
MS.gene05311.t1 MTR_6g011110 68.345 278 85 2 171 446 1 277 1.42e-134 388
MS.gene05311.t1 MTR_3g006120 55.085 354 136 4 105 443 4 349 3.74e-132 385
MS.gene05311.t1 MTR_3g006100 54.342 357 140 4 105 446 4 352 3.10e-131 383
MS.gene05311.t1 MTR_3g006180 54.971 342 131 4 115 441 1 334 3.98e-125 367
MS.gene05311.t1 MTR_4g133320 55.031 318 118 4 144 446 1 308 9.59e-113 334
MS.gene05311.t1 MTR_7g083950 45.415 458 190 14 8 446 16 432 2.01e-111 335
MS.gene05311.t1 MTR_3g085910 55.864 324 71 5 104 404 2 276 5.41e-110 326
MS.gene05311.t1 MTR_3g045700 50.815 368 138 10 103 446 2 350 1.83e-104 314
MS.gene05311.t1 MTR_3g086480 61.832 262 89 4 178 438 2 253 6.91e-102 304
MS.gene05311.t1 MTR_7g070100 47.887 355 160 7 107 446 6 350 1.50e-96 294
MS.gene05311.t1 MTR_1g109340 40.000 445 159 10 7 445 12 354 4.31e-89 275
MS.gene05311.t1 MTR_1g100280 45.380 368 142 9 100 446 8 337 1.05e-87 271
MS.gene05311.t1 MTR_6g091850 83.168 101 17 0 346 446 1 101 1.68e-59 190
MS.gene05311.t1 MTR_1g089085 52.318 151 55 3 95 232 29 175 1.06e-41 146
MS.gene05311.t1 MTR_1g109520 49.091 165 74 4 284 445 19 176 8.01e-41 144
MS.gene05311.t1 MTR_1g089620 60.833 120 42 1 265 384 34 148 1.74e-37 138
MS.gene05311.t1 MTR_1g012330 51.449 138 64 1 171 305 1 138 6.25e-31 120
MS.gene05311.t1 MTR_1g109090 47.656 128 54 2 97 211 8 135 3.56e-30 114
MS.gene05311.t1 MTR_1g106050 58.586 99 29 1 105 191 2 100 1.93e-29 112
MS.gene05311.t1 MTR_0128s0040 41.791 134 64 3 134 255 3 134 1.21e-24 99.4
MS.gene05311.t1 MTR_6g023220 25.767 326 225 6 114 426 22 343 4.39e-23 100
MS.gene05311.t1 MTR_1g093300 53.191 94 42 1 268 361 7 98 6.13e-20 86.3
MS.gene05311.t1 MTR_3g006185 39.080 87 43 3 1 85 94 172 2.90e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 84 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATATTTCTTCTTGTAAA+TGG 0.163180 6.3:-74539941 None:intergenic
TTTGATGACCTCTTTCAATT+TGG 0.236098 6.3:+74539677 MS.gene05311:CDS
TCTTTGTTGGATTGCTTATT+CGG 0.252635 6.3:+74540105 MS.gene05311:CDS
GAGAATTTCATAAAATTTAT+TGG 0.253294 6.3:+74539720 MS.gene05311:CDS
TTATGTCAAGTTGGAATCTT+TGG 0.283864 6.3:+74540378 MS.gene05311:CDS
ATCAATCTCGAAATCTTTGT+TGG 0.320105 6.3:+74540092 MS.gene05311:CDS
ATGGCAAACGAACACGGAAT+TGG 0.323818 6.3:+74538803 MS.gene05311:CDS
GACATATTCCTCTGAGCCTT+TGG 0.328107 6.3:-74540029 None:intergenic
AGGATGAATAAGGTACTTAA+AGG 0.337066 6.3:+74538965 MS.gene05311:CDS
TTTGGACCAGTAATGGTTCT+TGG 0.342105 6.3:-74539760 None:intergenic
ATTCAAAGAAAGGATGAATA+AGG 0.348934 6.3:+74538955 MS.gene05311:CDS
ATGCCAGAATTCATTCTTCC+TGG 0.356806 6.3:+74540544 MS.gene05311:CDS
CGTTGTTGATGATCATTGAT+TGG 0.356815 6.3:-74539058 None:intergenic
AATTTATTGGAGGAATTTCA+TGG 0.361680 6.3:+74539733 MS.gene05311:CDS
CTTTGTTGGATTGCTTATTC+GGG 0.379417 6.3:+74540106 MS.gene05311:CDS
GGCATCCCAAGAACCATTAC+TGG 0.386873 6.3:+74539754 MS.gene05311:CDS
ATGTTCCATGTGGCAAATAC+AGG 0.390944 6.3:+74540497 MS.gene05311:CDS
TGCTTATTCGGGACCACATA+AGG 0.394621 6.3:+74540117 MS.gene05311:CDS
TCATTGATTGGGATTGGAAT+TGG 0.394903 6.3:-74539046 None:intergenic
TGGCAAACGAACACGGAATT+GGG 0.404920 6.3:+74538804 MS.gene05311:CDS
GGCAAATACAGGGTGTATAA+AGG 0.407440 6.3:+74540508 MS.gene05311:CDS
CAAAACAACGTTACGTAGAT+TGG 0.408142 6.3:+74540608 MS.gene05311:CDS
AAGAGAACTGTCAACTTCCT+TGG 0.409046 6.3:+74539621 MS.gene05311:CDS
TTGGACCAGTAATGGTTCTT+GGG 0.410638 6.3:-74539759 None:intergenic
TATGTCAAGTTGGAATCTTT+GGG 0.417393 6.3:+74540379 MS.gene05311:CDS
TACTTCCGATTTGCCTATCA+AGG 0.418933 6.3:+74539860 MS.gene05311:CDS
TGATGATCATTGATTGGGAT+TGG 0.430835 6.3:-74539052 None:intergenic
ACACCAGGAAGAATGAATTC+TGG 0.440652 6.3:-74540547 None:intergenic
GTTGTTGATGATCATTGATT+GGG 0.445628 6.3:-74539057 None:intergenic
AGAGAACTGTCAACTTCCTT+GGG 0.449563 6.3:+74539622 MS.gene05311:CDS
CTGGTGTAACTTCTAATTGT+TGG 0.451842 6.3:+74540254 MS.gene05311:CDS
GATCTGTGTTTCATGGTAAG+AGG 0.473039 6.3:+74538843 MS.gene05311:CDS
CACTGAGCAACCCGCGCATG+TGG 0.492803 6.3:+74540443 MS.gene05311:CDS
AGTGCTTAATATTATGTCAA+AGG 0.495744 6.3:+74540315 MS.gene05311:CDS
AAGACCCTTGATAGGCAAAT+CGG 0.497171 6.3:-74539865 None:intergenic
ACGCACCTTTGGACCAGTAA+TGG 0.499173 6.3:-74539767 None:intergenic
GGATGAATAAGGTACTTAAA+GGG 0.501407 6.3:+74538966 MS.gene05311:CDS
GTAGGGTTGGTTAGTTGTGA+TGG 0.506721 6.3:+74540283 MS.gene05311:CDS
ATACATTACCAAATTGAAAG+AGG 0.507339 6.3:-74539685 None:intergenic
CTAATTGTTGGTCACACGTA+GGG 0.509336 6.3:+74540266 MS.gene05311:CDS
CTCAGAGGAATATGTCTTGG+TGG 0.510484 6.3:+74540036 MS.gene05311:CDS
GAAGTAAGATCTGTGTTTCA+TGG 0.513154 6.3:+74538836 MS.gene05311:CDS
TTAGAACTGAAGAGTACTCC+TGG 0.514280 6.3:+74540235 MS.gene05311:CDS
ATCAACAACGAGGAGGTCAT+AGG 0.524747 6.3:+74539071 MS.gene05311:CDS
TACCATGGATTATGTCAAGT+TGG 0.524896 6.3:+74540369 MS.gene05311:CDS
CAAGCTGAGTATCCCTATGT+TGG 0.527658 6.3:-74540191 None:intergenic
ATCTGTGTTTCATGGTAAGA+GGG 0.535708 6.3:+74538844 MS.gene05311:CDS
TCCGCTAGAAGACCCTTGAT+AGG 0.537852 6.3:-74539873 None:intergenic
GAGCAGCAATAACTAGGATG+TGG 0.542005 6.3:-74540586 None:intergenic
AATTCCGTGTTCGTTTGCCA+TGG 0.544629 6.3:-74538801 None:intergenic
TTCTCATAACCCCACATGCG+CGG 0.556155 6.3:-74540454 None:intergenic
GTCACTGACAAGGCCAACAT+AGG 0.560597 6.3:+74540178 MS.gene05311:CDS
GAGTGATAAACTTTATGATG+AGG 0.561825 6.3:-74539821 None:intergenic
AGGCTCAGAGGAATATGTCT+TGG 0.563313 6.3:+74540033 MS.gene05311:CDS
TCTCATAACCCCACATGCGC+GGG 0.566108 6.3:-74540453 None:intergenic
GCAAAGTCAACAACCTTATG+TGG 0.569183 6.3:-74540130 None:intergenic
CTGAGCAACCCGCGCATGTG+GGG 0.569892 6.3:+74540445 MS.gene05311:CDS
GCCTCCATGGCAAACGAACA+CGG 0.570354 6.3:+74538797 None:intergenic
TACACCCTGTATTTGCCACA+TGG 0.574023 6.3:-74540502 None:intergenic
AATTTCATAAAATTTATTGG+AGG 0.580475 6.3:+74539723 MS.gene05311:CDS
ACTGAGCAACCCGCGCATGT+GGG 0.589599 6.3:+74540444 MS.gene05311:CDS
AGTTCACTGCGTGTAACACC+AGG 0.590897 6.3:-74540562 None:intergenic
TCACTGACAAGGCCAACATA+GGG 0.593942 6.3:+74540179 MS.gene05311:CDS
CGTATATGTTGTCACTGACA+AGG 0.598708 6.3:+74540168 MS.gene05311:CDS
TTCCAACTTGACATAATCCA+TGG 0.602044 6.3:-74540371 None:intergenic
GCCTATCAAGGGTCTTCTAG+CGG 0.602253 6.3:+74539872 MS.gene05311:CDS
CAACGAGGAGGTCATAGGAC+TGG 0.607727 6.3:+74539076 MS.gene05311:CDS
AAGAACCATTACTGGTCCAA+AGG 0.607946 6.3:+74539762 MS.gene05311:CDS
GATACGAAAATGCTTCATGT+AGG 0.610679 6.3:-74539017 None:intergenic
TGTTCCATGTGGCAAATACA+GGG 0.612242 6.3:+74540498 MS.gene05311:CDS
TTGTTGGTCACACGTAGGGT+TGG 0.615794 6.3:+74540270 MS.gene05311:CDS
ATATTTCATCATGACACGAA+AGG 0.624589 6.3:+74539895 MS.gene05311:CDS
ACTTCCGATTTGCCTATCAA+GGG 0.626406 6.3:+74539861 MS.gene05311:CDS
CAACAATTAGAAGTTACACC+AGG 0.627295 6.3:-74540253 None:intergenic
CAAGATAGACTTCTCTACCA+TGG 0.632047 6.3:+74540354 MS.gene05311:CDS
ACACGGAATTGGGTTTCTCG+AGG 0.651937 6.3:+74538814 MS.gene05311:CDS
TCTAATTGTTGGTCACACGT+AGG 0.652885 6.3:+74540265 MS.gene05311:CDS
ATGATGTCAAGCAAATCCCA+AGG 0.658074 6.3:-74539638 None:intergenic
TCGATAATCATTAATCCATG+TGG 0.658195 6.3:-74538909 None:intergenic
TTTGCCACATGGAACATCGA+GGG 0.658254 6.3:-74540491 None:intergenic
ATTTGCCACATGGAACATCG+AGG 0.704595 6.3:-74540492 None:intergenic
AATGATCATCAACAACGAGG+AGG 0.708383 6.3:+74539064 MS.gene05311:CDS
TATGCCCTCGATGTTCCATG+TGG 0.716778 6.3:+74540487 MS.gene05311:CDS
ATCAATGATCATCAACAACG+AGG 0.770468 6.3:+74539061 MS.gene05311:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTTTTTTATTTATTA+TGG - chr6.3:74539507-74539526 None:intergenic 0.0%
!! AAAAAATAGTAAAAACAAGA+AGG - chr6.3:74539425-74539444 None:intergenic 15.0%
!! AATTTCATAAAATTTATTGG+AGG + chr6.3:74539723-74539742 MS.gene05311:CDS 15.0%
!! GAGAATTTCATAAAATTTAT+TGG + chr6.3:74539720-74539739 MS.gene05311:CDS 15.0%
!!! ACTATTTTTTTCGAAAAAAT+GGG + chr6.3:74539435-74539454 MS.gene05311:intron 15.0%
!!! CTCATTTTCTATAATATTAT+GGG + chr6.3:74539480-74539499 MS.gene05311:intron 15.0%
!!! TACTATTTTTTTCGAAAAAA+TGG + chr6.3:74539434-74539453 MS.gene05311:intron 15.0%
!!! TTTTATCTGATATATGTTTT+GGG + chr6.3:74539120-74539139 MS.gene05311:intron 15.0%
!!! TTTTTATCTGATATATGTTT+TGG + chr6.3:74539119-74539138 MS.gene05311:intron 15.0%
!! CCATAATATTATAGAAAATG+AGG - chr6.3:74539482-74539501 None:intergenic 20.0%
!!! CCTCATTTTCTATAATATTA+TGG + chr6.3:74539479-74539498 MS.gene05311:intron 20.0%
!!! TTTTCTTCTCAACATTAAAA+CGG - chr6.3:74539258-74539277 None:intergenic 20.0%
! AATTTATTGGAGGAATTTCA+TGG + chr6.3:74539733-74539752 MS.gene05311:CDS 25.0%
! AGCATATTTCTTCTTGTAAA+TGG - chr6.3:74539944-74539963 None:intergenic 25.0%
! AGTGCTTAATATTATGTCAA+AGG + chr6.3:74540315-74540334 MS.gene05311:CDS 25.0%
! ATACATTACCAAATTGAAAG+AGG - chr6.3:74539688-74539707 None:intergenic 25.0%
! ATTCAAAGAAAGGATGAATA+AGG + chr6.3:74538955-74538974 MS.gene05311:CDS 25.0%
! CAGTTCAAAAATTCAAAGAA+AGG + chr6.3:74538945-74538964 MS.gene05311:CDS 25.0%
! TATATTATTCCTTTGTAGCT+AGG + chr6.3:74539150-74539169 MS.gene05311:intron 25.0%
!! ATGAAGTTCTAGCATAATTA+AGG - chr6.3:74539349-74539368 None:intergenic 25.0%
!! TTTTAGACATCTACAATATG+AGG + chr6.3:74540633-74540652 MS.gene05311:CDS 25.0%
!!! GGGCATAATCATTTAATTTT+TGG + chr6.3:74538986-74539005 MS.gene05311:CDS 25.0%
!!! TTGGTAATGTATGCAAAAAT+TGG + chr6.3:74539696-74539715 MS.gene05311:CDS 25.0%
AACATTAAAACGGCAGATAA+TGG - chr6.3:74539248-74539267 None:intergenic 30.0%
ATATTTCATCATGACACGAA+AGG + chr6.3:74539895-74539914 MS.gene05311:CDS 30.0%
ATCAATCTCGAAATCTTTGT+TGG + chr6.3:74540092-74540111 MS.gene05311:CDS 30.0%
CAAAGGACAAAAAAAGAAGA+AGG + chr6.3:74539595-74539614 MS.gene05311:intron 30.0%
GAGTGATAAACTTTATGATG+AGG - chr6.3:74539824-74539843 None:intergenic 30.0%
TAAAAACAAGAAGGTAGAAG+AGG - chr6.3:74539416-74539435 None:intergenic 30.0%
TCGATAATCATTAATCCATG+TGG - chr6.3:74538912-74538931 None:intergenic 30.0%
TCTTTGTTGGATTGCTTATT+CGG + chr6.3:74540105-74540124 MS.gene05311:CDS 30.0%
! AGGATGAATAAGGTACTTAA+AGG + chr6.3:74538965-74538984 MS.gene05311:CDS 30.0%
! ATTTGCTTGACATCATTTTG+AGG + chr6.3:74539645-74539664 MS.gene05311:CDS 30.0%
! GGATGAATAAGGTACTTAAA+GGG + chr6.3:74538966-74538985 MS.gene05311:CDS 30.0%
! TTTGATGACCTCTTTCAATT+TGG + chr6.3:74539677-74539696 MS.gene05311:CDS 30.0%
!! GTTGTTGATGATCATTGATT+GGG - chr6.3:74539060-74539079 None:intergenic 30.0%
!! TATGTCAAGTTGGAATCTTT+GGG + chr6.3:74540379-74540398 MS.gene05311:CDS 30.0%
!! TTATGTCAAGTTGGAATCTT+TGG + chr6.3:74540378-74540397 MS.gene05311:CDS 30.0%
!!! AGTGCATAATACTTTTTCGT+CGG - chr6.3:74540427-74540446 None:intergenic 30.0%
!!! ATCTGATATATGTTTTGGGA+AGG + chr6.3:74539124-74539143 MS.gene05311:intron 30.0%
!!! GGTATCAAGGTTTTAAATAC+AGG + chr6.3:74539171-74539190 MS.gene05311:intron 30.0%
ATCAATGATCATCAACAACG+AGG + chr6.3:74539061-74539080 MS.gene05311:CDS 35.0%
ATCTGTGTTTCATGGTAAGA+GGG + chr6.3:74538844-74538863 MS.gene05311:CDS 35.0%
ATTTCTTAACAACGCACCTT+TGG - chr6.3:74539781-74539800 None:intergenic 35.0%
CAAAACAACGTTACGTAGAT+TGG + chr6.3:74540608-74540627 MS.gene05311:CDS 35.0%
CAACAATTAGAAGTTACACC+AGG - chr6.3:74540256-74540275 None:intergenic 35.0%
CTGGTGTAACTTCTAATTGT+TGG + chr6.3:74540254-74540273 MS.gene05311:CDS 35.0%
CTTTGTTGGATTGCTTATTC+GGG + chr6.3:74540106-74540125 MS.gene05311:CDS 35.0%
GAAGTAAGATCTGTGTTTCA+TGG + chr6.3:74538836-74538855 MS.gene05311:CDS 35.0%
GATACGAAAATGCTTCATGT+AGG - chr6.3:74539020-74539039 None:intergenic 35.0%
GCATAATTAAGGATGTTTCG+CGG - chr6.3:74539338-74539357 None:intergenic 35.0%
TCACAACAAAGGATGATGTT+AGG - chr6.3:74539573-74539592 None:intergenic 35.0%
TGTCCTTTGTATCACAACAA+AGG - chr6.3:74539584-74539603 None:intergenic 35.0%
TTCCAACTTGACATAATCCA+TGG - chr6.3:74540374-74540393 None:intergenic 35.0%
! CATCCTTTGTTGTGATACAA+AGG + chr6.3:74539578-74539597 MS.gene05311:intron 35.0%
! GAATTTTTGAACTGCAAGTC+TGG - chr6.3:74538939-74538958 None:intergenic 35.0%
! TACCATGGATTATGTCAAGT+TGG + chr6.3:74540369-74540388 MS.gene05311:CDS 35.0%
! TCATTGATTGGGATTGGAAT+TGG - chr6.3:74539049-74539068 None:intergenic 35.0%
! TGATGATCATTGATTGGGAT+TGG - chr6.3:74539055-74539074 None:intergenic 35.0%
!! CGTTGTTGATGATCATTGAT+TGG - chr6.3:74539061-74539080 None:intergenic 35.0%
!! TGTTTTGAGCAGCAATAACT+AGG - chr6.3:74540595-74540614 None:intergenic 35.0%
!!! TACATGCTTTTAAGACCACA+TGG + chr6.3:74538894-74538913 MS.gene05311:CDS 35.0%
AAGAACCATTACTGGTCCAA+AGG + chr6.3:74539762-74539781 MS.gene05311:CDS 40.0%
AAGACCCTTGATAGGCAAAT+CGG - chr6.3:74539868-74539887 None:intergenic 40.0%
AAGAGAACTGTCAACTTCCT+TGG + chr6.3:74539621-74539640 MS.gene05311:CDS 40.0%
AATGATCATCAACAACGAGG+AGG + chr6.3:74539064-74539083 MS.gene05311:CDS 40.0%
ACACCAGGAAGAATGAATTC+TGG - chr6.3:74540550-74540569 None:intergenic 40.0%
ACCTTGATACCTAGCTACAA+AGG - chr6.3:74539162-74539181 None:intergenic 40.0%
AGAGAACTGTCAACTTCCTT+GGG + chr6.3:74539622-74539641 MS.gene05311:CDS 40.0%
ATGATGTCAAGCAAATCCCA+AGG - chr6.3:74539641-74539660 None:intergenic 40.0%
ATGCCAGAATTCATTCTTCC+TGG + chr6.3:74540544-74540563 MS.gene05311:CDS 40.0%
ATGTTCCATGTGGCAAATAC+AGG + chr6.3:74540497-74540516 MS.gene05311:CDS 40.0%
CAAGATAGACTTCTCTACCA+TGG + chr6.3:74540354-74540373 MS.gene05311:CDS 40.0%
CGATATCGGTCGTTACAAAA+CGG + chr6.3:74539204-74539223 MS.gene05311:intron 40.0%
CGTATATGTTGTCACTGACA+AGG + chr6.3:74540168-74540187 MS.gene05311:CDS 40.0%
CTAATTGTTGGTCACACGTA+GGG + chr6.3:74540266-74540285 MS.gene05311:CDS 40.0%
GATCTGTGTTTCATGGTAAG+AGG + chr6.3:74538843-74538862 MS.gene05311:CDS 40.0%
GCAAAGTCAACAACCTTATG+TGG - chr6.3:74540133-74540152 None:intergenic 40.0%
GGCAAATACAGGGTGTATAA+AGG + chr6.3:74540508-74540527 MS.gene05311:CDS 40.0%
TCCTTTGTAGCTAGGTATCA+AGG + chr6.3:74539158-74539177 MS.gene05311:intron 40.0%
TCTAATTGTTGGTCACACGT+AGG + chr6.3:74540265-74540284 MS.gene05311:CDS 40.0%
TGTTCCATGTGGCAAATACA+GGG + chr6.3:74540498-74540517 MS.gene05311:CDS 40.0%
TTGGACCAGTAATGGTTCTT+GGG - chr6.3:74539762-74539781 None:intergenic 40.0%
TTTGGACCAGTAATGGTTCT+TGG - chr6.3:74539763-74539782 None:intergenic 40.0%
! ACTTCCGATTTGCCTATCAA+GGG + chr6.3:74539861-74539880 MS.gene05311:CDS 40.0%
! TACTTCCGATTTGCCTATCA+AGG + chr6.3:74539860-74539879 MS.gene05311:CDS 40.0%
! TTTTGTAACGACCGATATCG+TGG - chr6.3:74539204-74539223 None:intergenic 40.0%
!! TTAGAACTGAAGAGTACTCC+TGG + chr6.3:74540235-74540254 MS.gene05311:CDS 40.0%
!!! GTCTTGCTTGCTTTTTCCAA+AGG + chr6.3:74540013-74540032 MS.gene05311:CDS 40.0%
AAAACGGTATAGGCAGCCAA+CGG + chr6.3:74539220-74539239 MS.gene05311:intron 45.0%
AGGCTCAGAGGAATATGTCT+TGG + chr6.3:74540033-74540052 MS.gene05311:CDS 45.0%
ATCAACAACGAGGAGGTCAT+AGG + chr6.3:74539071-74539090 MS.gene05311:CDS 45.0%
ATGGCAAACGAACACGGAAT+TGG + chr6.3:74538803-74538822 MS.gene05311:CDS 45.0%
ATTTGCCACATGGAACATCG+AGG - chr6.3:74540495-74540514 None:intergenic 45.0%
CAAGCTGAGTATCCCTATGT+TGG - chr6.3:74540194-74540213 None:intergenic 45.0%
CAGGAACGATACCACGATAT+CGG + chr6.3:74539190-74539209 MS.gene05311:intron 45.0%
CGGTCGTTACAAAACGGTAT+AGG + chr6.3:74539210-74539229 MS.gene05311:intron 45.0%
CTCAGAGGAATATGTCTTGG+TGG + chr6.3:74540036-74540055 MS.gene05311:CDS 45.0%
GACATATTCCTCTGAGCCTT+TGG - chr6.3:74540032-74540051 None:intergenic 45.0%
GAGCAGCAATAACTAGGATG+TGG - chr6.3:74540589-74540608 None:intergenic 45.0%
TACACCCTGTATTTGCCACA+TGG - chr6.3:74540505-74540524 None:intergenic 45.0%
TCACTGACAAGGCCAACATA+GGG + chr6.3:74540179-74540198 MS.gene05311:CDS 45.0%
TGCTTATTCGGGACCACATA+AGG + chr6.3:74540117-74540136 MS.gene05311:CDS 45.0%
TGGCAAACGAACACGGAATT+GGG + chr6.3:74538804-74538823 MS.gene05311:CDS 45.0%
TTTGCCACATGGAACATCGA+GGG - chr6.3:74540494-74540513 None:intergenic 45.0%
!! GTAGGGTTGGTTAGTTGTGA+TGG + chr6.3:74540283-74540302 MS.gene05311:CDS 45.0%
!!! TGCTTTTTCCAAAGGCTCAG+AGG + chr6.3:74540021-74540040 MS.gene05311:CDS 45.0%
ACACGGAATTGGGTTTCTCG+AGG + chr6.3:74538814-74538833 MS.gene05311:CDS 50.0%
ACGCACCTTTGGACCAGTAA+TGG - chr6.3:74539770-74539789 None:intergenic 50.0%
AGTTCACTGCGTGTAACACC+AGG - chr6.3:74540565-74540584 None:intergenic 50.0%
GCCTATCAAGGGTCTTCTAG+CGG + chr6.3:74539872-74539891 MS.gene05311:CDS 50.0%
GGCATCCCAAGAACCATTAC+TGG + chr6.3:74539754-74539773 MS.gene05311:CDS 50.0%
GTCACTGACAAGGCCAACAT+AGG + chr6.3:74540178-74540197 MS.gene05311:CDS 50.0%
TATGCCCTCGATGTTCCATG+TGG + chr6.3:74540487-74540506 MS.gene05311:CDS 50.0%
TCCGCTAGAAGACCCTTGAT+AGG - chr6.3:74539876-74539895 None:intergenic 50.0%
TTCTCATAACCCCACATGCG+CGG - chr6.3:74540457-74540476 None:intergenic 50.0%
TTGTTGGTCACACGTAGGGT+TGG + chr6.3:74540270-74540289 MS.gene05311:CDS 50.0%
! ACGGCAGATAATGGTACCGT+TGG - chr6.3:74539239-74539258 None:intergenic 50.0%
AGGATGTTTCGCGGCCGTAA+CGG - chr6.3:74539329-74539348 None:intergenic 55.0%
CAACGAGGAGGTCATAGGAC+TGG + chr6.3:74539076-74539095 MS.gene05311:CDS 55.0%
CCTGTCACGGCGATACGTAA+CGG - chr6.3:74539295-74539314 None:intergenic 55.0%
TCTCATAACCCCACATGCGC+GGG - chr6.3:74540456-74540475 None:intergenic 55.0%
ACTGAGCAACCCGCGCATGT+GGG + chr6.3:74540444-74540463 MS.gene05311:CDS 60.0%
ATGTTTCGCGGCCGTAACGG+CGG - chr6.3:74539326-74539345 None:intergenic 60.0%
CCGTTACGTATCGCCGTGAC+AGG + chr6.3:74539292-74539311 MS.gene05311:intron 60.0%
AGGCGCGAAACCCGCCGTTA+CGG + chr6.3:74539312-74539331 MS.gene05311:intron 65.0%
CACTGAGCAACCCGCGCATG+TGG + chr6.3:74540443-74540462 MS.gene05311:CDS 65.0%
CTGAGCAACCCGCGCATGTG+GGG + chr6.3:74540445-74540464 MS.gene05311:CDS 65.0%
TGTTTCGCGGCCGTAACGGC+GGG - chr6.3:74539325-74539344 None:intergenic 65.0%
! GGCGGGTTTCGCGCCTGTCA+CGG - chr6.3:74539308-74539327 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 74538803 74540678 74538803 ID=MS.gene05311
chr6.3 mRNA 74538803 74540678 74538803 ID=MS.gene05311.t1;Parent=MS.gene05311
chr6.3 exon 74538803 74539097 74538803 ID=MS.gene05311.t1.exon1;Parent=MS.gene05311.t1
chr6.3 CDS 74538803 74539097 74538803 ID=cds.MS.gene05311.t1;Parent=MS.gene05311.t1
chr6.3 exon 74539600 74539783 74539600 ID=MS.gene05311.t1.exon2;Parent=MS.gene05311.t1
chr6.3 CDS 74539600 74539783 74539600 ID=cds.MS.gene05311.t1;Parent=MS.gene05311.t1
chr6.3 exon 74539817 74540678 74539817 ID=MS.gene05311.t1.exon3;Parent=MS.gene05311.t1
chr6.3 CDS 74539817 74540678 74539817 ID=cds.MS.gene05311.t1;Parent=MS.gene05311.t1
Gene Sequence

>MS.gene05311

ATGGCAAACGAACACGGAATTGGGTTTCTCGAGGAAGTAAGATCTGTGTTTCATGGTAAGAGGGAAGAGTATGAAGAGTTTATAAAAGACATACATGCTTTTAAGACCACATGGATTAATGATTATCGAATCACCAGACTTGCAGTTCAAAAATTCAAAGAAAGGATGAATAAGGTACTTAAAGGGCATAATCATTTAATTTTTGGATTCAACGCCTACATGAAGCATTTTCGTATCACACTTCCAATTCCAATCCCAATCAATGATCATCAACAACGAGGAGGTCATAGGACTGGTTAGTACTTGTTTTTACTATTTTTTATCTGATATATGTTTTGGGAAGGTCGTATATTATTCCTTTGTAGCTAGGTATCAAGGTTTTAAATACAGGAACGATACCACGATATCGGTCGTTACAAAACGGTATAGGCAGCCAACGGTACCATTATCTGCCGTTTTAATGTTGAGAAGAAAAATCACACTAAAATGCCGTTACGTATCGCCGTGACAGGCGCGAAACCCGCCGTTACGGCCGCGAAACATCCTTAATTATGCTAGAACTTCATATATGTTTCTTCTTTTCAGCTTCATTATTCAATCTACATTGCTTCCTCTTCTACCTTCTTGTTTTTACTATTTTTTTCGAAAAAATGGGAAAATAAATATTTCTTTATCTCCTCATTTTCTATAATATTATGGGACCATAATAAATAAAAAAATTATATGTTTTAGAAATATAGAGAAAAAAAAAACATGTTTGTAAATCACCTAACATCATCCTTTGTTGTGATACAAAGGACAAAAAAAGAAGAAGGTAGAAGAGAACTGTCAACTTCCTTGGGATTTGCTTGACATCATTTTGAGGAATCTAGATTTTGATGACCTCTTTCAATTTGGTAATGTATGCAAAAATTGGCGAGAATTTCATAAAATTTATTGGAGGAATTTCATGGCATCCCAAGAACCATTACTGGTCCAAAGGTGCGTTGTTAAGAAATCTTTCATCTTCTTCAGCTTACCTCATCATAAAGTTTATCACTCAAAGATGATCAATAACTACTTCCGATTTGCCTATCAAGGGTCTTCTAGCGGATATTTCATCATGACACGAAAGGATAATTCATTTATACTTATTAATCCATTTACAAGAAGAAATATGCTAATCAATAACTCAGTCTTCAATGTTGATTTCAAACATTTTTCTTGCAAAGTCTTGCTTGCTTTTTCCAAAGGCTCAGAGGAATATGTCTTGGTGGTTTTATGTATTAATTCTGATAATTTGCATGTCTATCAATCTCGAAATCTTTGTTGGATTGCTTATTCGGGACCACATAAGGTTGTTGACTTTGCTGTTTTAAATTATATCGTATATGTTGTCACTGACAAGGCCAACATAGGGATACTCAGCTTGAAATATGCAAATATAAATTTTTTAGAACTGAAGAGTACTCCTGGTGTAACTTCTAATTGTTGGTCACACGTAGGGTTGGTTAGTTGTGATGGATATCTTTTAGTGCTTAATATTATGTCAAAGGTAACGTTTAACGTGTACAAGATAGACTTCTCTACCATGGATTATGTCAAGTTGGAATCTTTGGGTGACATTGCAATATTTTATCTTCCGACGAAAAAGTATTATGCACTGAGCAACCCGCGCATGTGGGGTTATGAGAACAATTCTATCTATGCCCTCGATGTTCCATGTGGCAAATACAGGGTGTATAAAGGAGATAACAATAAAATGCCAGAATTCATTCTTCCTGGTGTTACACGCAGTGAACTTCCACATCCTAGTTATTGCTGCTCAAAACAACGTTACGTAGATTGGTGTTTTAGACATCTACAATATGAGGTAGATTATACTCTCATTGAGTAG
Protein sequence

>MS.gene05311.t1

MANEHGIGFLEEVRSVFHGKREEYEEFIKDIHAFKTTWINDYRITRLAVQKFKERMNKVLKGHNHLIFGFNAYMKHFRITLPIPIPINDHQQRGGHRTGQKKKKVEENCQLPWDLLDIILRNLDFDDLFQFGNVCKNWREFHKIYWRNFMASQEPLLVQSLPHHKVYHSKMINNYFRFAYQGSSSGYFIMTRKDNSFILINPFTRRNMLINNSVFNVDFKHFSCKVLLAFSKGSEEYVLVVLCINSDNLHVYQSRNLCWIAYSGPHKVVDFAVLNYIVYVVTDKANIGILSLKYANINFLELKSTPGVTSNCWSHVGLVSCDGYLLVLNIMSKVTFNVYKIDFSTMDYVKLESLGDIAIFYLPTKKYYALSNPRMWGYENNSIYALDVPCGKYRVYKGDNNKMPEFILPGVTRSELPHPSYCCSKQRYVDWCFRHLQYEVDYTLIE