AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53925

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53925.t1 MTR_3g006120 94.886 352 18 0 1 352 1 352 0.0 691
MS.gene53925.t1 MTR_3g006100 92.264 349 27 0 1 349 1 349 0.0 670
MS.gene53925.t1 MTR_3g006180 92.604 338 25 0 14 351 1 338 0.0 649
MS.gene53925.t1 MTR_3g006010 86.932 352 46 0 1 352 118 469 0.0 639
MS.gene53925.t1 MTR_3g006140 73.837 344 90 0 4 347 26 369 0.0 533
MS.gene53925.t1 MTR_4g133490 70.520 346 90 2 9 349 7 345 2.55e-168 473
MS.gene53925.t1 MTR_4g133320 70.684 307 86 2 44 349 2 305 9.31e-146 414
MS.gene53925.t1 MTR_3g068220 58.611 360 132 4 1 349 1 354 1.08e-138 398
MS.gene53925.t1 MTR_6g091830 55.866 358 145 5 4 352 102 455 2.87e-135 393
MS.gene53925.t1 MTR_6g092140 55.587 358 146 5 4 352 102 455 5.25e-134 390
MS.gene53925.t1 MTR_7g070100 58.092 346 141 4 8 352 8 350 7.81e-129 373
MS.gene53925.t1 MTR_3g045700 55.899 356 147 5 1 352 1 350 1.73e-127 369
MS.gene53925.t1 MTR_7g083950 55.028 358 146 8 1 352 84 432 7.52e-119 350
MS.gene53925.t1 MTR_6g088340 55.732 314 126 5 48 352 124 433 1.00e-109 327
MS.gene53925.t1 MTR_1g100280 50.000 358 137 7 4 352 13 337 5.51e-105 311
MS.gene53925.t1 MTR_6g011110 56.272 279 110 3 83 352 2 277 4.57e-100 297
MS.gene53925.t1 MTR_1g109340 60.079 253 93 3 103 351 106 354 1.45e-94 286
MS.gene53925.t1 MTR_3g085910 47.664 321 109 6 6 316 5 276 1.18e-91 275
MS.gene53925.t1 MTR_3g086480 56.641 256 105 4 90 344 3 253 6.18e-86 260
MS.gene53925.t1 MTR_1g109520 59.494 158 60 1 198 351 19 176 3.20e-57 184
MS.gene53925.t1 MTR_1g089620 60.448 134 50 1 175 308 30 160 3.42e-47 162
MS.gene53925.t1 MTR_1g012330 63.504 137 50 0 83 219 2 138 1.98e-45 156
MS.gene53925.t1 MTR_1g089085 57.447 141 55 3 4 143 39 175 2.49e-45 153
MS.gene53925.t1 MTR_1g109090 50.388 129 63 1 1 128 13 141 3.62e-35 126
MS.gene53925.t1 MTR_1g106050 58.879 107 42 1 4 108 2 108 1.38e-34 124
MS.gene53925.t1 MTR_6g023220 27.759 299 208 6 9 305 18 310 4.33e-29 115
MS.gene53925.t1 MTR_1g093300 52.336 107 51 0 176 282 1 107 1.76e-27 105
MS.gene53925.t1 MTR_0128s0040 42.105 133 75 2 33 165 3 133 3.88e-25 99.4
MS.gene53925.t1 MTR_6g091850 43.564 101 51 2 258 352 1 101 3.89e-24 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTCTTTCTTCTTGTAAA+TGG 0.145840 3.2:+940492 None:intergenic
TGTCTATCAGTCTGGAAATT+TGG 0.158326 3.2:-940346 MS.gene53925:CDS
TTCTGGCACGTTGCTTATTC+TGG 0.158749 3.2:-940573 MS.gene53925:CDS
GGGAATTTCATAAAACTTAT+TGG 0.162427 3.2:-940716 MS.gene53925:CDS
ATAAGAAGAATTGACTTTAA+AGG 0.192960 3.2:+940455 None:intergenic
CTATCATGCTTAATGTATTT+TGG 0.196702 3.2:+939883 None:intergenic
TTTGAATAAGTAATGGTTCT+TGG 0.234598 3.2:+940676 None:intergenic
TACCATGTCTTGCTTGCTTT+TGG 0.255664 3.2:-940426 MS.gene53925:CDS
AAGATGGTTGTAAAATTTCT+TGG 0.292218 3.2:-940815 MS.gene53925:CDS
TTCCATCTTGACAAAATTCA+TGG 0.296900 3.2:+940059 None:intergenic
ACAACATGTAAAGTGTGTTT+AGG 0.310726 3.2:-939922 MS.gene53925:CDS
GTCTATCAGTCTGGAAATTT+GGG 0.319305 3.2:-940345 MS.gene53925:CDS
CTTTCAAAACCCCACCTTCT+CGG 0.331626 3.2:+939976 None:intergenic
GGAAAAGGATTTCTTATCAT+TGG 0.332744 3.2:+940647 None:intergenic
CAACATGTAAAGTGTGTTTA+GGG 0.332853 3.2:-939921 MS.gene53925:CDS
GTCGCTTCATGTAAAAGTTC+TGG 0.337852 3.2:-940378 MS.gene53925:CDS
AAAGGAAAACGCCTACTTGC+TGG 0.351938 3.2:-939845 MS.gene53925:CDS
ATCAGTCTGGAAATTTGGGC+TGG 0.359141 3.2:-940341 MS.gene53925:CDS
CCAAGGCGATCAATCAATTC+TGG 0.360139 3.2:-940590 MS.gene53925:CDS
TACACCTATGTTGGCCTTGT+CGG 0.389440 3.2:+940242 None:intergenic
AGATGGTTGTAAAATTTCTT+GGG 0.404155 3.2:-940814 MS.gene53925:CDS
TTTCAAAACCCCACCTTCTC+GGG 0.416348 3.2:+939977 None:intergenic
CAATAACCAGATTACTCAAC+TGG 0.422972 3.2:+939781 None:intergenic
GATGACCTCTTTCAATTCGC+TGG 0.428564 3.2:-940756 MS.gene53925:CDS
TAAACACACTTTACATGTTG+TGG 0.439177 3.2:+939924 None:intergenic
AGCGGATATTTGATCATGAC+AGG 0.440460 3.2:-940543 MS.gene53925:CDS
GTCATCGAAATCTAGATCTC+TGG 0.451722 3.2:+940773 None:intergenic
TCGCTGGTGTGTGCAAGAAT+TGG 0.467150 3.2:-940740 MS.gene53925:CDS
TTTGTCAAGATGGAAACTTT+GGG 0.468280 3.2:-940051 MS.gene53925:CDS
AGTTTGTATGTCTATCAGTC+TGG 0.474620 3.2:-940354 MS.gene53925:CDS
AAAACGCCTACTTGCTGGAT+TGG 0.477038 3.2:-939840 MS.gene53925:CDS
CTCAGGATGGTTAGTTGTGA+TGG 0.492017 3.2:-940147 MS.gene53925:CDS
TCCATTTACAAGAAGAAAGA+AGG 0.500617 3.2:-940493 MS.gene53925:CDS
TCGGTCAGCTATGCTGATGA+AGG 0.509137 3.2:+940626 None:intergenic
TCAGTCTGGAAATTTGGGCT+GGG 0.521625 3.2:-940340 MS.gene53925:CDS
CCAGAATTGATTGATCGCCT+TGG 0.527310 3.2:+940590 None:intergenic
GTAAACATTCAATATTTCCT+CGG 0.528220 3.2:+940098 None:intergenic
AGCTATGCTGATGAAGGAAA+AGG 0.531287 3.2:+940632 None:intergenic
ACAAAGTCAACAACCTTCCA+TGG 0.536142 3.2:+940294 None:intergenic
TAACATCTACCTCACTCCTC+AGG 0.540088 3.2:-940164 MS.gene53925:CDS
TGTTGTGGATGAAAGATTGA+TGG 0.544678 3.2:+939939 None:intergenic
AGTGATTCATATTGCTTCCG+AGG 0.546777 3.2:-940115 MS.gene53925:CDS
CAAGTCAAGTACACCTATGT+TGG 0.548742 3.2:+940233 None:intergenic
ATTCAACAATGGAAAGTCCA+TGG 0.553899 3.2:-940311 MS.gene53925:CDS
GCTTATTCTGGCTGTTCTAG+CGG 0.565805 3.2:-940561 MS.gene53925:CDS
CATTGAGCAACCCGAGAAGG+TGG 0.569290 3.2:-939987 MS.gene53925:CDS
AACAATGGAAAGTCCATGGA+AGG 0.572932 3.2:-940307 MS.gene53925:CDS
ACACACCAGCGAATTGAAAG+AGG 0.578254 3.2:+940751 None:intergenic
AGTGAGGTAGATGTTACATC+GGG 0.579813 3.2:+940171 None:intergenic
ATGCATTGAGCAACCCGAGA+AGG 0.585187 3.2:-939990 MS.gene53925:CDS
GCAATGAATAACATACAAGA+TGG 0.590425 3.2:-940831 MS.gene53925:CDS
CTTTAGACATCTACACTATG+AGG 0.594856 3.2:-939815 MS.gene53925:CDS
CTGGTGTGTGCAAGAATTGG+AGG 0.595567 3.2:-940737 MS.gene53925:CDS
TGCCAAAAGCAAGCAAGACA+TGG 0.610086 3.2:+940424 None:intergenic
GAGTGAGGTAGATGTTACAT+CGG 0.616275 3.2:+940170 None:intergenic
ATTGAGCAACCCGAGAAGGT+GGG 0.616952 3.2:-939986 MS.gene53925:CDS
CCGAAAAGTTTATCACTCCA+AGG 0.620313 3.2:-940607 MS.gene53925:CDS
AAAGCACCAATCCAGCAAGT+AGG 0.620337 3.2:+939834 None:intergenic
TTGAGCAACCCGAGAAGGTG+GGG 0.622323 3.2:-939985 MS.gene53925:CDS
CTGGGTTACATATTCAACAA+TGG 0.624667 3.2:-940322 MS.gene53925:CDS
GTCACCGACAAGGCCAACAT+AGG 0.627828 3.2:-940246 MS.gene53925:CDS
ATCTACCTCACTCCTCAGGA+TGG 0.644112 3.2:-940160 MS.gene53925:CDS
TAGAACTCGAGTTCCTCCAA+AGG 0.651017 3.2:-939863 MS.gene53925:CDS
TAGACATCTACACTATGAGG+TGG 0.658182 3.2:-939812 MS.gene53925:CDS
TATATACGTTGTCACCGACA+AGG 0.661708 3.2:-940256 MS.gene53925:CDS
ACTAACCATCCTGAGGAGTG+AGG 0.672997 3.2:+940155 None:intergenic
GCGGATATTTGATCATGACA+GGG 0.678093 3.2:-940542 MS.gene53925:CDS
TGGTGTGTGCAAGAATTGGA+GGG 0.700448 3.2:-940736 MS.gene53925:CDS
AACATGTAAAGTGTGTTTAG+GGG 0.701783 3.2:-939920 MS.gene53925:CDS
CGGATATTTGATCATGACAG+GGG 0.702309 3.2:-940541 MS.gene53925:CDS
CATCACAACTAACCATCCTG+AGG 0.720834 3.2:+940148 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTAAAAATTTTATATCTG+TGG + chr3.2:940417-940436 None:intergenic 15.0%
!!! ATAAGAAGAATTGACTTTAA+AGG + chr3.2:940168-940187 None:intergenic 20.0%
! AACTTATTGGAAAAAGTTCT+TGG - chr3.2:939917-939936 MS.gene53925:CDS 25.0%
! AAGATGGTTGTAAAATTTCT+TGG - chr3.2:939805-939824 MS.gene53925:CDS 25.0%
! AGATGGTTGTAAAATTTCTT+GGG - chr3.2:939806-939825 MS.gene53925:CDS 25.0%
! GGGAATTTCATAAAACTTAT+TGG - chr3.2:939904-939923 MS.gene53925:CDS 25.0%
! GTAAACATTCAATATTTCCT+CGG + chr3.2:940525-940544 None:intergenic 25.0%
! TTTGAATAAGTAATGGTTCT+TGG + chr3.2:939947-939966 None:intergenic 25.0%
!! CTATCATGCTTAATGTATTT+TGG + chr3.2:940740-940759 None:intergenic 25.0%
!! GAAGACTTTTGAATAAGTAA+TGG + chr3.2:939954-939973 None:intergenic 25.0%
!!! ATTGCATTATTTTATGCTTC+GGG - chr3.2:940596-940615 MS.gene53925:CDS 25.0%
!!! CATTGCATTATTTTATGCTT+CGG - chr3.2:940595-940614 MS.gene53925:CDS 25.0%
AACATGTAAAGTGTGTTTAG+GGG - chr3.2:940700-940719 MS.gene53925:CDS 30.0%
ACAACATGTAAAGTGTGTTT+AGG - chr3.2:940698-940717 MS.gene53925:CDS 30.0%
ACCTTCTTTCTTCTTGTAAA+TGG + chr3.2:940131-940150 None:intergenic 30.0%
CAACATGTAAAGTGTGTTTA+GGG - chr3.2:940699-940718 MS.gene53925:CDS 30.0%
GGAAAAGGATTTCTTATCAT+TGG + chr3.2:939976-939995 None:intergenic 30.0%
TAAACACACTTTACATGTTG+TGG + chr3.2:940699-940718 None:intergenic 30.0%
TCCATTTACAAGAAGAAAGA+AGG - chr3.2:940127-940146 MS.gene53925:CDS 30.0%
TTCCATCTTGACAAAATTCA+TGG + chr3.2:940564-940583 None:intergenic 30.0%
TTTGTCAAGATGGAAACTTT+GGG - chr3.2:940569-940588 MS.gene53925:CDS 30.0%
! AACCATGAATTTTGTCAAGA+TGG - chr3.2:940559-940578 MS.gene53925:CDS 30.0%
! TTTTGTCAAGATGGAAACTT+TGG - chr3.2:940568-940587 MS.gene53925:CDS 30.0%
!! TTGCATTATTTTATGCTTCG+GGG - chr3.2:940597-940616 MS.gene53925:CDS 30.0%
AGTTTGTATGTCTATCAGTC+TGG - chr3.2:940266-940285 MS.gene53925:CDS 35.0%
ATTCAACAATGGAAAGTCCA+TGG - chr3.2:940309-940328 MS.gene53925:CDS 35.0%
CTGGGTTACATATTCAACAA+TGG - chr3.2:940298-940317 MS.gene53925:CDS 35.0%
CTTTAGACATCTACACTATG+AGG - chr3.2:940805-940824 MS.gene53925:CDS 35.0%
GTCTATCAGTCTGGAAATTT+GGG - chr3.2:940275-940294 MS.gene53925:CDS 35.0%
TGTCTATCAGTCTGGAAATT+TGG - chr3.2:940274-940293 MS.gene53925:CDS 35.0%
TGTTGTGGATGAAAGATTGA+TGG + chr3.2:940684-940703 None:intergenic 35.0%
! CCTTGGAGTGATAAACTTTT+CGG + chr3.2:940016-940035 None:intergenic 35.0%
!! TGCATTATTTTATGCTTCGG+GGG - chr3.2:940598-940617 MS.gene53925:CDS 35.0%
AACAATGGAAAGTCCATGGA+AGG - chr3.2:940313-940332 MS.gene53925:CDS 40.0%
ACAAAGTCAACAACCTTCCA+TGG + chr3.2:940329-940348 None:intergenic 40.0%
AGCGGATATTTGATCATGAC+AGG - chr3.2:940077-940096 MS.gene53925:CDS 40.0%
AGCTATGCTGATGAAGGAAA+AGG + chr3.2:939991-940010 None:intergenic 40.0%
AGTGAGGTAGATGTTACATC+GGG + chr3.2:940452-940471 None:intergenic 40.0%
AGTGATTCATATTGCTTCCG+AGG - chr3.2:940505-940524 MS.gene53925:CDS 40.0%
CAAGTCAAGTACACCTATGT+TGG + chr3.2:940390-940409 None:intergenic 40.0%
CCGAAAAGTTTATCACTCCA+AGG - chr3.2:940013-940032 MS.gene53925:CDS 40.0%
CGGATATTTGATCATGACAG+GGG - chr3.2:940079-940098 MS.gene53925:CDS 40.0%
GAGTGAGGTAGATGTTACAT+CGG + chr3.2:940453-940472 None:intergenic 40.0%
GCGGATATTTGATCATGACA+GGG - chr3.2:940078-940097 MS.gene53925:CDS 40.0%
GTCATCGAAATCTAGATCTC+TGG + chr3.2:939850-939869 None:intergenic 40.0%
GTCGCTTCATGTAAAAGTTC+TGG - chr3.2:940242-940261 MS.gene53925:CDS 40.0%
TAGACATCTACACTATGAGG+TGG - chr3.2:940808-940827 MS.gene53925:CDS 40.0%
TATATACGTTGTCACCGACA+AGG - chr3.2:940364-940383 MS.gene53925:CDS 40.0%
! TACCATGTCTTGCTTGCTTT+TGG - chr3.2:940194-940213 MS.gene53925:CDS 40.0%
AAAGGAAAACGCCTACTTGC+TGG - chr3.2:940775-940794 MS.gene53925:CDS 45.0%
ACACACCAGCGAATTGAAAG+AGG + chr3.2:939872-939891 None:intergenic 45.0%
ATCAGTCTGGAAATTTGGGC+TGG - chr3.2:940279-940298 MS.gene53925:CDS 45.0%
CATCACAACTAACCATCCTG+AGG + chr3.2:940475-940494 None:intergenic 45.0%
CCAAGGCGATCAATCAATTC+TGG - chr3.2:940030-940049 MS.gene53925:CDS 45.0%
CCAGAATTGATTGATCGCCT+TGG + chr3.2:940033-940052 None:intergenic 45.0%
CTTTCAAAACCCCACCTTCT+CGG + chr3.2:940647-940666 None:intergenic 45.0%
GATGACCTCTTTCAATTCGC+TGG - chr3.2:939864-939883 MS.gene53925:CDS 45.0%
TAACATCTACCTCACTCCTC+AGG - chr3.2:940456-940475 MS.gene53925:CDS 45.0%
TAGAACTCGAGTTCCTCCAA+AGG - chr3.2:940757-940776 MS.gene53925:CDS 45.0%
TCAGTCTGGAAATTTGGGCT+GGG - chr3.2:940280-940299 MS.gene53925:CDS 45.0%
TGCCAAAAGCAAGCAAGACA+TGG + chr3.2:940199-940218 None:intergenic 45.0%
TGGTGTGTGCAAGAATTGGA+GGG - chr3.2:939884-939903 MS.gene53925:CDS 45.0%
TTCTGGCACGTTGCTTATTC+TGG - chr3.2:940047-940066 MS.gene53925:CDS 45.0%
TTTCAAAACCCCACCTTCTC+GGG + chr3.2:940646-940665 None:intergenic 45.0%
! AAAACGCCTACTTGCTGGAT+TGG - chr3.2:940780-940799 MS.gene53925:CDS 45.0%
! AAAGCACCAATCCAGCAAGT+AGG + chr3.2:940789-940808 None:intergenic 45.0%
! AAGTAGGCGTTTTCCTTTGG+AGG + chr3.2:940773-940792 None:intergenic 45.0%
! AGCAAGTAGGCGTTTTCCTT+TGG + chr3.2:940776-940795 None:intergenic 45.0%
! CTCAGGATGGTTAGTTGTGA+TGG - chr3.2:940473-940492 MS.gene53925:CDS 45.0%
! GCTTATTCTGGCTGTTCTAG+CGG - chr3.2:940059-940078 MS.gene53925:CDS 45.0%
!! GTCTTGCTTGCTTTTGGCAA+AGG - chr3.2:940200-940219 MS.gene53925:CDS 45.0%
!! TACACCTATGTTGGCCTTGT+CGG + chr3.2:940381-940400 None:intergenic 45.0%
ACTAACCATCCTGAGGAGTG+AGG + chr3.2:940468-940487 None:intergenic 50.0%
ATCTACCTCACTCCTCAGGA+TGG - chr3.2:940460-940479 MS.gene53925:CDS 50.0%
ATGCATTGAGCAACCCGAGA+AGG - chr3.2:940630-940649 MS.gene53925:CDS 50.0%
ATTGAGCAACCCGAGAAGGT+GGG - chr3.2:940634-940653 MS.gene53925:CDS 50.0%
CTGGTGTGTGCAAGAATTGG+AGG - chr3.2:939883-939902 MS.gene53925:CDS 50.0%
TCGCTGGTGTGTGCAAGAAT+TGG - chr3.2:939880-939899 MS.gene53925:CDS 50.0%
TCGGTCAGCTATGCTGATGA+AGG + chr3.2:939997-940016 None:intergenic 50.0%
! TGCTTTTGGCAAAGGCTCAG+AGG - chr3.2:940208-940227 MS.gene53925:CDS 50.0%
CATTGAGCAACCCGAGAAGG+TGG - chr3.2:940633-940652 MS.gene53925:CDS 55.0%
GTCACCGACAAGGCCAACAT+AGG - chr3.2:940374-940393 MS.gene53925:CDS 55.0%
TTGAGCAACCCGAGAAGGTG+GGG - chr3.2:940635-940654 MS.gene53925:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 939792 940850 939792 ID=MS.gene53925
chr3.2 mRNA 939792 940850 939792 ID=MS.gene53925.t1;Parent=MS.gene53925
chr3.2 exon 939792 940850 939792 ID=MS.gene53925.t1.exon1;Parent=MS.gene53925.t1
chr3.2 CDS 939792 940850 939792 ID=cds.MS.gene53925.t1;Parent=MS.gene53925.t1
Gene Sequence

>MS.gene53925

ATGAATAACATACAAGATGGTTGTAAAATTTCTTGGGATATGCTTGATCTCATTTCCAGAGATCTAGATTTCGATGACCTCTTTCAATTCGCTGGTGTGTGCAAGAATTGGAGGGAATTTCATAAAACTTATTGGAAAAAGTTCTTGGCATTCCAAGAACCATTACTTATTCAAAAGTCTTCCAATGATAAGAAATCCTTTTCCTTCATCAGCATAGCTGACCGAAAAGTTTATCACTCCAAGGCGATCAATCAATTCTGGCACGTTGCTTATTCTGGCTGTTCTAGCGGATATTTGATCATGACAGGGGACAATAATTCTTTTCTTCTATTGAATCCATTTACAAGAAGAAAGAAGGTAATCAATACATCAACCTTTAAAGTCAATTCTTCTTATTTTGCTTACCATGTCTTGCTTGCTTTTGGCAAAGGCTCAGAGGAATTTGTCTTAGTCGCTTCATGTAAAAGTTCTGGTAGTTTGTATGTCTATCAGTCTGGAAATTTGGGCTGGGTTACATATTCAACAATGGAAAGTCCATGGAAGGTTGTTGACTTTGTTGTTTTGCATCATACTATATACGTTGTCACCGACAAGGCCAACATAGGTGTACTTGACTTGAATTCCACAGATATAAAATTTTTAGAACTGAAGAGTACACCCGATGTAACATCTACCTCACTCCTCAGGATGGTTAGTTGTGATGGACAACTTTTAGTGATTCATATTGCTTCCGAGGAAATATTGAATGTTTACAAGATAGACTTCTCAACCATGAATTTTGTCAAGATGGAAACTTTGGGTGACATTGCATTATTTTATGCTTCGGGGGAATACTTCTATGCATTGAGCAACCCGAGAAGGTGGGGTTTTGAAAGCAACTCTTTGTATGCCATCAATCTTTCATCCACAACATGTAAAGTGTGTTTAGGGGATGATAACGAACTGCCAAAATACATTAAGCATGATAGAACTCGAGTTCCTCCAAAGGAAAACGCCTACTTGCTGGATTGGTGCTTTAGACATCTACACTATGAGGTGGATCTTTCTCCAGTTGAGTAA
Protein sequence

>MS.gene53925.t1

MNNIQDGCKISWDMLDLISRDLDFDDLFQFAGVCKNWREFHKTYWKKFLAFQEPLLIQKSSNDKKSFSFISIADRKVYHSKAINQFWHVAYSGCSSGYLIMTGDNNSFLLLNPFTRRKKVINTSTFKVNSSYFAYHVLLAFGKGSEEFVLVASCKSSGSLYVYQSGNLGWVTYSTMESPWKVVDFVVLHHTIYVVTDKANIGVLDLNSTDIKFLELKSTPDVTSTSLLRMVSCDGQLLVIHIASEEILNVYKIDFSTMNFVKMETLGDIALFYASGEYFYALSNPRRWGFESNSLYAINLSSTTCKVCLGDDNELPKYIKHDRTRVPPKENAYLLDWCFRHLHYEVDLSPVE