AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67877

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67877.t1 MTR_7g083950 60.135 444 146 6 12 451 16 432 1.13e-171 489
MS.gene67877.t1 MTR_3g045700 64.835 364 110 4 90 451 3 350 1.02e-153 440
MS.gene67877.t1 MTR_3g006010 48.025 481 193 10 9 451 8 469 4.30e-131 387
MS.gene67877.t1 MTR_1g100280 57.453 369 113 6 87 451 9 337 8.93e-123 361
MS.gene67877.t1 MTR_6g091830 45.957 470 201 15 11 451 10 455 4.05e-117 350
MS.gene67877.t1 MTR_6g092140 45.745 470 202 15 11 451 10 455 1.25e-116 349
MS.gene67877.t1 MTR_4g133490 56.284 366 136 8 91 451 2 348 5.26e-115 341
MS.gene67877.t1 MTR_3g006120 52.174 368 150 8 91 451 4 352 1.16e-112 335
MS.gene67877.t1 MTR_3g006100 51.630 368 152 8 91 451 4 352 6.33e-112 333
MS.gene67877.t1 MTR_3g006180 52.925 359 139 9 101 450 1 338 1.29e-108 325
MS.gene67877.t1 MTR_3g006140 50.802 374 158 8 84 450 19 373 6.65e-107 322
MS.gene67877.t1 MTR_7g070100 49.727 366 156 10 93 451 6 350 4.51e-103 311
MS.gene67877.t1 MTR_1g109340 64.344 244 84 2 210 450 111 354 1.52e-101 307
MS.gene67877.t1 MTR_1g109340 45.562 169 54 5 1 153 2 148 1.10e-29 119
MS.gene67877.t1 MTR_3g068220 49.067 375 154 10 90 449 3 355 9.23e-97 295
MS.gene67877.t1 MTR_4g133320 53.517 327 128 8 130 451 1 308 1.09e-90 278
MS.gene67877.t1 MTR_6g088340 38.655 476 199 17 11 451 16 433 2.06e-79 253
MS.gene67877.t1 MTR_3g085910 43.109 341 116 9 89 417 1 275 1.12e-71 228
MS.gene67877.t1 MTR_6g011110 45.423 284 132 8 184 451 1 277 4.10e-64 208
MS.gene67877.t1 MTR_3g086480 47.674 258 123 6 192 443 2 253 3.50e-61 200
MS.gene67877.t1 MTR_1g109520 63.354 161 49 2 297 450 19 176 6.48e-58 189
MS.gene67877.t1 MTR_1g093300 86.667 105 14 0 275 379 1 105 2.05e-57 186
MS.gene67877.t1 MTR_1g089620 64.800 125 38 2 274 398 30 148 6.83e-43 153
MS.gene67877.t1 MTR_1g106050 69.912 113 18 3 91 203 2 98 3.02e-40 141
MS.gene67877.t1 MTR_1g089085 50.000 180 59 8 72 244 20 175 1.41e-38 138
MS.gene67877.t1 MTR_1g109090 53.459 159 55 6 83 238 8 150 2.95e-37 133
MS.gene67877.t1 MTR_1g093310 78.824 85 12 1 1 85 1 79 7.65e-37 130
MS.gene67877.t1 MTR_1g012330 51.079 139 63 4 184 318 1 138 4.07e-28 112
MS.gene67877.t1 MTR_8g062700 41.606 137 60 2 1 137 36 152 4.46e-22 100
MS.gene67877.t1 MTR_8g062700 41.606 137 60 2 1 137 36 152 4.46e-22 100
MS.gene67877.t1 MTR_5g036670 56.522 92 38 1 1 92 37 126 1.46e-21 98.6
MS.gene67877.t1 MTR_1g105960 64.179 67 24 0 11 77 3 69 5.00e-21 88.2
MS.gene67877.t1 MTR_6g023220 25.344 363 236 14 95 447 17 354 3.37e-19 89.4
MS.gene67877.t1 MTR_6g091850 42.308 104 48 2 357 451 1 101 2.41e-18 80.5
MS.gene67877.t1 MTR_6g008995 39.535 129 58 2 9 137 37 145 2.86e-18 87.4
MS.gene67877.t1 MTR_6g008970 40.146 137 62 3 1 137 57 173 5.53e-18 87.4
MS.gene67877.t1 MTR_4g133300 57.746 71 30 0 12 82 175 245 8.21e-18 83.2
MS.gene67877.t1 MTR_4g133300 50.704 71 32 1 9 79 12 79 2.55e-11 64.3
MS.gene67877.t1 MTR_4g133300 44.000 75 42 0 6 80 86 160 2.65e-11 63.9
MS.gene67877.t1 MTR_4g133470 57.746 71 30 0 12 82 175 245 1.18e-17 82.8
MS.gene67877.t1 MTR_4g133470 50.704 71 32 1 9 79 12 79 2.96e-11 63.9
MS.gene67877.t1 MTR_1g105955 58.462 65 27 0 11 75 18 82 1.57e-17 80.9
MS.gene67877.t1 MTR_1g105955 50.000 66 33 0 12 77 98 163 1.53e-13 69.3
MS.gene67877.t1 MTR_1g091047 56.250 64 27 1 9 71 9 72 1.40e-16 74.7
MS.gene67877.t1 MTR_3g006065 48.039 102 41 3 1 91 72 172 1.48e-16 78.2
MS.gene67877.t1 MTR_3g006065 53.521 71 30 1 9 79 8 75 1.63e-13 69.3
MS.gene67877.t1 MTR_1g104940 43.820 89 47 1 9 94 14 102 3.18e-16 75.1
MS.gene67877.t1 MTR_0128s0040 36.667 150 73 6 120 265 3 134 8.45e-16 74.3
MS.gene67877.t1 MTR_3g006185 51.282 78 37 1 9 85 98 175 1.03e-15 75.5
MS.gene67877.t1 MTR_3g006185 50.685 73 35 1 9 80 8 80 4.42e-12 64.7
MS.gene67877.t1 MTR_8g062350 43.000 100 53 2 9 106 14 111 1.35e-15 75.9
MS.gene67877.t1 MTR_3g006125 52.564 78 36 1 9 85 180 257 1.47e-15 76.6
MS.gene67877.t1 MTR_3g006125 50.685 73 35 1 9 80 89 161 8.73e-12 65.5
MS.gene67877.t1 MTR_3g006150 52.778 72 31 1 9 80 8 76 2.89e-14 70.9
MS.gene67877.t1 MTR_3g006150 54.930 71 31 1 9 78 90 160 6.63e-14 69.7
MS.gene67877.t1 MTR_1g104980 43.529 85 44 1 12 92 73 157 3.00e-14 71.2
MS.gene67877.t1 MTR_7g013410 52.239 67 32 0 13 79 2 68 3.46e-14 70.1
MS.gene67877.t1 MTR_1g109550 52.941 68 31 1 9 76 16 82 5.20e-14 69.7
MS.gene67877.t1 MTR_1g100260 47.619 84 40 2 10 89 25 108 8.38e-14 68.6
MS.gene67877.t1 MTR_1g104960 45.588 68 37 0 11 78 101 168 1.66e-13 68.9
MS.gene67877.t1 MTR_6g008995 38.318 107 46 2 31 137 2 88 6.12e-13 70.5
MS.gene67877.t1 MTR_1g105010 42.029 69 40 0 9 77 16 84 7.36e-13 65.5
MS.gene67877.t1 MTR_3g006105 50.649 77 37 1 9 84 99 175 1.17e-12 66.6
MS.gene67877.t1 MTR_3g006105 47.436 78 40 1 9 85 8 85 2.52e-12 65.9
MS.gene67877.t1 MTR_1g023820 60.000 50 20 0 39 88 17 66 1.56e-12 64.7
MS.gene67877.t1 MTR_2g097830 50.704 71 32 1 9 79 70 137 1.82e-12 65.9
MS.gene67877.t1 MTR_1g106560 52.542 59 13 1 28 86 16 59 3.32e-12 61.6
MS.gene67877.t1 MTR_1g104937 39.535 86 51 1 9 93 12 97 1.52e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67877.t1 AT1G24190 57.778 90 34 2 1 90 8 93 1.53e-20 95.5
MS.gene67877.t1 AT1G24190 57.778 90 34 2 1 90 8 93 1.60e-20 95.5
MS.gene67877.t1 AT1G24190 57.778 90 34 2 1 90 8 93 1.64e-20 95.5
MS.gene67877.t1 AT3G01320 43.478 138 55 5 1 137 51 166 6.27e-20 93.6
MS.gene67877.t1 AT3G01320 43.478 138 55 5 1 137 51 166 7.48e-20 93.6
MS.gene67877.t1 AT1G70060 60.256 78 29 1 1 78 8 83 8.25e-19 90.1
MS.gene67877.t1 AT1G70060 60.256 78 29 1 1 78 8 83 8.25e-19 90.1
MS.gene67877.t1 AT5G15020 40.310 129 57 2 9 137 52 160 1.11e-18 89.7
MS.gene67877.t1 AT5G15020 40.310 129 57 2 9 137 52 160 1.14e-18 89.7
MS.gene67877.t1 AT1G59890 54.321 81 37 0 9 89 45 125 2.68e-18 88.6
MS.gene67877.t1 AT1G59890 54.321 81 37 0 9 89 45 125 2.81e-18 88.6
MS.gene67877.t1 AT1G59890 54.321 81 37 0 9 89 45 125 2.91e-18 88.6
MS.gene67877.t1 AT1G59890 54.321 81 37 0 9 89 45 125 2.92e-18 88.6
MS.gene67877.t1 AT1G10450 54.286 70 32 0 9 78 33 102 9.08e-15 77.4
MS.gene67877.t1 AT1G10450 54.286 70 32 0 9 78 33 102 9.08e-15 77.4
MS.gene67877.t1 AT1G10450 54.286 70 32 0 9 78 84 153 9.13e-15 77.4
MS.gene67877.t1 AT1G27250 51.471 68 33 0 9 76 21 88 1.21e-14 71.2
MS.gene67877.t1 AT1G24200 50.725 69 34 0 9 77 14 82 1.61e-14 72.4
MS.gene67877.t1 AT5G15025 50.000 70 35 0 9 78 47 116 7.38e-14 68.6
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 7.58e-14 74.7
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 9.02e-14 74.3
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 9.02e-14 74.3
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 9.07e-14 74.3
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 9.07e-14 74.3
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 9.49e-14 74.3
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 9.59e-14 74.3
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 9.67e-14 74.3
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 9.75e-14 74.3
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 308 376 9.76e-14 74.3
MS.gene67877.t1 AT4G12020 54.286 70 31 1 9 78 241 309 9.96e-14 74.3
MS.gene67877.t1 AT1G70030 42.553 94 53 1 9 101 22 115 1.53e-13 67.8
MS.gene67877.t1 AT1G70030 36.641 131 62 3 9 139 22 131 2.60e-13 67.4
MS.gene67877.t1 AT1G70030 42.553 94 53 1 9 101 11 104 6.51e-13 67.0
MS.gene67877.t1 AT5G15040 45.833 72 39 0 12 83 6 77 1.14e-12 63.9
MS.gene67877.t1 AT5G15040 45.833 72 39 0 12 83 6 77 1.14e-12 63.9
MS.gene67877.t1 AT5G15040 45.833 72 39 0 12 83 6 77 1.14e-12 63.9
MS.gene67877.t1 AT1G27220 45.333 75 41 0 9 83 14 88 1.19e-12 66.6
MS.gene67877.t1 AT5G35610 42.000 100 55 2 13 111 14 111 1.52e-12 65.9

Find 84 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTCTTTGTTCTTGTAAA+TGG 0.109229 2.4:-57953853 None:intergenic
AGGGCATACAGATTTAATTT+TGG 0.178085 2.4:+57953381 MS.gene67877:CDS
AATCTACTGGAGAAATTTCT+TGG 0.234930 2.4:+57953597 MS.gene67877:CDS
GACGATCTCTTTCAGTTTGC+CGG 0.249250 2.4:+57953544 MS.gene67877:CDS
AACCATGAATTATGTTAAAA+TGG 0.251991 2.4:+57954275 MS.gene67877:CDS
AAATTTCTTGGAATCCTTCT+TGG 0.269320 2.4:+57953609 MS.gene67877:CDS
CTCTCATATCCCCACAAGTT+TGG 0.270711 2.4:-57954369 None:intergenic
AATATATGTTGTCACTATTA+AGG 0.297389 2.4:+57954080 MS.gene67877:CDS
TATATGCGATGAACAACTTT+TGG 0.306580 2.4:+57954200 MS.gene67877:CDS
TGACAACATATATTATATTA+TGG 0.318413 2.4:-57954071 None:intergenic
CAGGTTCTTTCATGTTTGAT+TGG 0.323947 2.4:+57954496 MS.gene67877:CDS
CCTTCTTTGTTCTTGTAAAT+GGG 0.329673 2.4:-57953852 None:intergenic
TATTCAACAATGAAAGATAA+AGG 0.331753 2.4:+57954024 MS.gene67877:CDS
CTTGCCATGGTTGCTAATAA+AGG 0.331817 2.4:-57953686 None:intergenic
GTTATAGTAATAAATTTCAT+TGG 0.336574 2.4:-57954166 None:intergenic
GCAGTAAAGGAGCTGTTTAA+AGG 0.344725 2.4:+57953361 MS.gene67877:CDS
ATCGTCAAAATCTAGTGTTT+TGG 0.353186 2.4:-57953527 None:intergenic
TTTATGTTGCTGATAAGTTC+AGG 0.367338 2.4:+57954322 MS.gene67877:CDS
CAAGTTGTAGTGTGTATTCA+TGG 0.371052 2.4:+57954424 MS.gene67877:CDS
ATTACGGTTTCAACTCTTGA+TGG 0.382679 2.4:+57954471 MS.gene67877:CDS
ATTCAACAATGAAAGATAAA+GGG 0.382953 2.4:+57954025 MS.gene67877:CDS
AAGAATGTGTTCAAGATAGA+AGG 0.385935 2.4:+57954249 MS.gene67877:CDS
TTGGTTGGGTATGCTAATAA+AGG 0.390018 2.4:-57953719 None:intergenic
TTATGTTAAAATGGAAACTT+TGG 0.395281 2.4:+57954284 MS.gene67877:CDS
TTGCCGGTGTATGCAAGAAT+TGG 0.398043 2.4:+57953560 MS.gene67877:CDS
AAACGTTCTGATGAGTTTCA+AGG 0.401132 2.4:+57953306 MS.gene67877:CDS
GAAGAACCATTACTACTTCA+AGG 0.405321 2.4:+57953637 MS.gene67877:CDS
AGTTTATTGCTTGAAGATGA+TGG 0.413789 2.4:+57953744 MS.gene67877:CDS
CCATTCGTCTCAAGTTGAAA+TGG 0.424230 2.4:-57953445 None:intergenic
TATGTTAAAATGGAAACTTT+GGG 0.446419 2.4:+57954285 MS.gene67877:CDS
ATACTCAGAAGAATTTATCT+TGG 0.447982 2.4:+57953942 MS.gene67877:CDS
GTTTCAACTCTTGATGGAAC+AGG 0.448641 2.4:+57954477 MS.gene67877:CDS
TGATTTCATGTTCCTTTGGC+AGG 0.451994 2.4:-57953417 None:intergenic
AGTGTGATTTCATGTTCCTT+TGG 0.456196 2.4:-57953421 None:intergenic
AGAGAATTGTGAACTTCCTT+CGG 0.458702 2.4:+57953486 MS.gene67877:CDS
CATCGCATATAACGAGCCTT+AGG 0.459211 2.4:-57954188 None:intergenic
TTATAGTAATAAATTTCATT+GGG 0.460815 2.4:-57954165 None:intergenic
CATGATCGCTTGCCACTACA+TGG 0.464402 2.4:-57953661 None:intergenic
AGGTGTTGTGGCGGCAGTAA+AGG 0.465057 2.4:+57953348 MS.gene67877:CDS
TATAATTGTTAATTCCGATA+GGG 0.469816 2.4:-57953885 None:intergenic
TTATGTTGCTGATAAGTTCA+GGG 0.470327 2.4:+57954323 MS.gene67877:CDS
AAAATGTAACATAGTTATAG+TGG 0.472211 2.4:-57953780 None:intergenic
CAGTAAAGGAGCTGTTTAAA+GGG 0.479626 2.4:+57953362 MS.gene67877:CDS
TTCATGTTCCTTTGGCAGGA+AGG 0.481994 2.4:-57953413 None:intergenic
GTCTATCAATCTCGAAATTG+TGG 0.493729 2.4:+57953991 MS.gene67877:CDS
TGGTGGTTAATTTAAGGCAT+AGG 0.495040 2.4:+57954220 MS.gene67877:CDS
CCGGTGTATGCAAGAATTGG+AGG 0.497149 2.4:+57953563 MS.gene67877:CDS
CCATTTCAACTTGAGACGAA+TGG 0.510483 2.4:+57953445 MS.gene67877:CDS
AAATTTATTACTATAACCTA+AGG 0.513173 2.4:+57954172 MS.gene67877:CDS
CCCATTTACAAGAACAAAGA+AGG 0.515788 2.4:+57953852 MS.gene67877:CDS
TCACTATTAAGGCCAACATA+GGG 0.520688 2.4:+57954091 MS.gene67877:CDS
ATAATTGTTAATTCCGATAG+GGG 0.524435 2.4:-57953884 None:intergenic
CATGGACCTTGAAGTAGTAA+TGG 0.525481 2.4:-57953643 None:intergenic
CTTTATTAGCAACCATGGCA+AGG 0.525891 2.4:+57953687 MS.gene67877:CDS
ATCAATCTCGAAATTGTGGA+TGG 0.526711 2.4:+57953995 MS.gene67877:CDS
TGGTTCTTCAGATGCCAAGA+AGG 0.541790 2.4:-57953623 None:intergenic
GTCACTATTAAGGCCAACAT+AGG 0.545424 2.4:+57954090 MS.gene67877:CDS
TTTATTAGCAACCATGGCAA+GGG 0.548269 2.4:+57953688 MS.gene67877:CDS
CTCAAACCACTACGATCGAC+TGG 0.548987 2.4:+57953222 MS.gene67877:CDS
TGTTCCTTTATTAGCAACCA+TGG 0.550724 2.4:+57953682 MS.gene67877:CDS
GATCATAGAATTGATATAAG+AGG 0.552187 2.4:+57953328 MS.gene67877:CDS
TTATAATTGTTAATTCCGAT+AGG 0.553099 2.4:-57953886 None:intergenic
ATTGAGCAATCCAAACTTGT+GGG 0.554665 2.4:+57954359 MS.gene67877:CDS
CGGTGTATGCAAGAATTGGA+GGG 0.575557 2.4:+57953564 MS.gene67877:CDS
CAAGCTGAGTACCCCTATGT+TGG 0.581306 2.4:-57954103 None:intergenic
AATGAAAGATAAAGGGGTCG+TGG 0.582610 2.4:+57954032 MS.gene67877:CDS
CATTGAGCAATCCAAACTTG+TGG 0.583187 2.4:+57954358 MS.gene67877:CDS
AATTGATATAAGAGGTGTTG+TGG 0.583198 2.4:+57953336 MS.gene67877:CDS
AAGGTAATCGATACCCCTAT+CGG 0.585550 2.4:+57953871 MS.gene67877:CDS
TTCAACAATGAAAGATAAAG+GGG 0.603126 2.4:+57954026 MS.gene67877:CDS
CTACTTCAAGGTCCATGTAG+TGG 0.613260 2.4:+57953649 MS.gene67877:CDS
ATTCAACACCTTCCTGCCAA+AGG 0.618562 2.4:+57953405 MS.gene67877:CDS
TCAATCTCGAAATTGTGGAT+GGG 0.618953 2.4:+57953996 MS.gene67877:CDS
GTATTTGTCAAAAGCAAGCA+AGG 0.624429 2.4:-57953923 None:intergenic
TGATATAAGAGGTGTTGTGG+CGG 0.630919 2.4:+57953339 MS.gene67877:CDS
CACTATTAAGGCCAACATAG+GGG 0.633566 2.4:+57954092 MS.gene67877:CDS
TGTCAAAAGCAAGCAAGGCA+CGG 0.637399 2.4:-57953918 None:intergenic
ATGATATCAAGCACATCCGA+AGG 0.639846 2.4:-57953502 None:intergenic
CGATCGACTGGCATATCTTG+CGG 0.643609 2.4:+57953234 MS.gene67877:CDS
AGGAACATGATCCCTTGCCA+TGG 0.650753 2.4:-57953699 None:intergenic
CCTCCAATTCTTGCATACAC+CGG 0.651637 2.4:-57953563 None:intergenic
AAGTTGTAGTGTGTATTCAT+GGG 0.670579 2.4:+57954425 MS.gene67877:CDS
TTGAGCAATCCAAACTTGTG+GGG 0.674083 2.4:+57954360 MS.gene67877:CDS
GATATGCCAGTCGATCGTAG+TGG 0.714548 2.4:-57953228 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAGTAATAAATTTCATT+GGG - chr2.4:57954168-57954187 None:intergenic 10.0%
!! AAATTTATTACTATAACCTA+AGG + chr2.4:57954172-57954191 MS.gene67877:CDS 15.0%
!! GTTATAGTAATAAATTTCAT+TGG - chr2.4:57954169-57954188 None:intergenic 15.0%
!! TGACAACATATATTATATTA+TGG - chr2.4:57954074-57954093 None:intergenic 15.0%
!! AAAATGTAACATAGTTATAG+TGG - chr2.4:57953783-57953802 None:intergenic 20.0%
!! AACCATGAATTATGTTAAAA+TGG + chr2.4:57954275-57954294 MS.gene67877:CDS 20.0%
!! AATATATGTTGTCACTATTA+AGG + chr2.4:57954080-57954099 MS.gene67877:CDS 20.0%
!! ATTCAACAATGAAAGATAAA+GGG + chr2.4:57954025-57954044 MS.gene67877:CDS 20.0%
!! TATAATTGTTAATTCCGATA+GGG - chr2.4:57953888-57953907 None:intergenic 20.0%
!! TATGTTAAAATGGAAACTTT+GGG + chr2.4:57954285-57954304 MS.gene67877:CDS 20.0%
!! TATTCAACAATGAAAGATAA+AGG + chr2.4:57954024-57954043 MS.gene67877:CDS 20.0%
!! TTATAATTGTTAATTCCGAT+AGG - chr2.4:57953889-57953908 None:intergenic 20.0%
!! TTATGTTAAAATGGAAACTT+TGG + chr2.4:57954284-57954303 MS.gene67877:CDS 20.0%
!!! TTCCATTTTAACATAATTCA+TGG - chr2.4:57954280-57954299 None:intergenic 20.0%
! AAAGTTGCAAAAATGCATTA+CGG + chr2.4:57954455-57954474 MS.gene67877:CDS 25.0%
! ATAATTGTTAATTCCGATAG+GGG - chr2.4:57953887-57953906 None:intergenic 25.0%
! ATACTCAGAAGAATTTATCT+TGG + chr2.4:57953942-57953961 MS.gene67877:CDS 25.0%
! GATCATAGAATTGATATAAG+AGG + chr2.4:57953328-57953347 MS.gene67877:CDS 25.0%
! TTCAACAATGAAAGATAAAG+GGG + chr2.4:57954026-57954045 MS.gene67877:CDS 25.0%
!! AACTATGTTACATTTTCTAG+TGG + chr2.4:57953787-57953806 MS.gene67877:CDS 25.0%
!! TCTTCAAGCAATAAACTTTT+TGG - chr2.4:57953741-57953760 None:intergenic 25.0%
!!! AACTTTTGGTGGTTAATTTA+AGG + chr2.4:57954214-57954233 MS.gene67877:CDS 25.0%
!!! AAGCAATAAACTTTTTGGTT+GGG - chr2.4:57953736-57953755 None:intergenic 25.0%
!!! TTCTAGTGGATATTTTATCA+CGG + chr2.4:57953801-57953820 MS.gene67877:CDS 25.0%
AAATTTCTTGGAATCCTTCT+TGG + chr2.4:57953609-57953628 MS.gene67877:CDS 30.0%
AAGAATGTGTTCAAGATAGA+AGG + chr2.4:57954249-57954268 MS.gene67877:CDS 30.0%
AAGTTGTAGTGTGTATTCAT+GGG + chr2.4:57954425-57954444 MS.gene67877:CDS 30.0%
AATCTACTGGAGAAATTTCT+TGG + chr2.4:57953597-57953616 MS.gene67877:CDS 30.0%
ACCTTCTTTGTTCTTGTAAA+TGG - chr2.4:57953856-57953875 None:intergenic 30.0%
AGTTTATTGCTTGAAGATGA+TGG + chr2.4:57953744-57953763 MS.gene67877:CDS 30.0%
CCTTCTTTGTTCTTGTAAAT+GGG - chr2.4:57953855-57953874 None:intergenic 30.0%
TTATGTTGCTGATAAGTTCA+GGG + chr2.4:57954323-57954342 MS.gene67877:CDS 30.0%
TTTATGTTGCTGATAAGTTC+AGG + chr2.4:57954322-57954341 MS.gene67877:CDS 30.0%
! ATCGTCAAAATCTAGTGTTT+TGG - chr2.4:57953530-57953549 None:intergenic 30.0%
! GGGTTTTTCATAAAATCTAC+TGG + chr2.4:57953584-57953603 MS.gene67877:CDS 30.0%
! TATATGCGATGAACAACTTT+TGG + chr2.4:57954200-57954219 MS.gene67877:CDS 30.0%
!! AATTGATATAAGAGGTGTTG+TGG + chr2.4:57953336-57953355 MS.gene67877:CDS 30.0%
!! AGGGCATACAGATTTAATTT+TGG + chr2.4:57953381-57953400 MS.gene67877:CDS 30.0%
!! CAAGCAATAAACTTTTTGGT+TGG - chr2.4:57953737-57953756 None:intergenic 30.0%
!! GGGCATACAGATTTAATTTT+GGG + chr2.4:57953382-57953401 MS.gene67877:CDS 30.0%
AAACGTTCTGATGAGTTTCA+AGG + chr2.4:57953306-57953325 MS.gene67877:CDS 35.0%
AGAGAATTGTGAACTTCCTT+CGG + chr2.4:57953486-57953505 MS.gene67877:CDS 35.0%
AGTGTGATTTCATGTTCCTT+TGG - chr2.4:57953424-57953443 None:intergenic 35.0%
ATCAATCTCGAAATTGTGGA+TGG + chr2.4:57953995-57954014 MS.gene67877:CDS 35.0%
ATTACGGTTTCAACTCTTGA+TGG + chr2.4:57954471-57954490 MS.gene67877:CDS 35.0%
ATTGAGCAATCCAAACTTGT+GGG + chr2.4:57954359-57954378 MS.gene67877:CDS 35.0%
CAAGTTGTAGTGTGTATTCA+TGG + chr2.4:57954424-57954443 MS.gene67877:CDS 35.0%
CAGGTTCTTTCATGTTTGAT+TGG + chr2.4:57954496-57954515 MS.gene67877:CDS 35.0%
CAGTAAAGGAGCTGTTTAAA+GGG + chr2.4:57953362-57953381 MS.gene67877:CDS 35.0%
CCCATTTACAAGAACAAAGA+AGG + chr2.4:57953852-57953871 MS.gene67877:CDS 35.0%
GAAGAACCATTACTACTTCA+AGG + chr2.4:57953637-57953656 MS.gene67877:CDS 35.0%
GACGAATGGAAAAACATTGA+AGG + chr2.4:57953459-57953478 MS.gene67877:CDS 35.0%
GTATTTGTCAAAAGCAAGCA+AGG - chr2.4:57953926-57953945 None:intergenic 35.0%
GTCTATCAATCTCGAAATTG+TGG + chr2.4:57953991-57954010 MS.gene67877:CDS 35.0%
TCAATCTCGAAATTGTGGAT+GGG + chr2.4:57953996-57954015 MS.gene67877:CDS 35.0%
TCACTATTAAGGCCAACATA+GGG + chr2.4:57954091-57954110 MS.gene67877:CDS 35.0%
TGTTCCTTTATTAGCAACCA+TGG + chr2.4:57953682-57953701 MS.gene67877:CDS 35.0%
TTGGTTGGGTATGCTAATAA+AGG - chr2.4:57953722-57953741 None:intergenic 35.0%
TTTATTAGCAACCATGGCAA+GGG + chr2.4:57953688-57953707 MS.gene67877:CDS 35.0%
! TGGATATTTTATCACGGTGA+GGG + chr2.4:57953807-57953826 MS.gene67877:CDS 35.0%
! TGGTGGTTAATTTAAGGCAT+AGG + chr2.4:57954220-57954239 MS.gene67877:CDS 35.0%
AAGGTAATCGATACCCCTAT+CGG + chr2.4:57953871-57953890 MS.gene67877:CDS 40.0%
AATGAAAGATAAAGGGGTCG+TGG + chr2.4:57954032-57954051 MS.gene67877:CDS 40.0%
ATGATATCAAGCACATCCGA+AGG - chr2.4:57953505-57953524 None:intergenic 40.0%
CACTATTAAGGCCAACATAG+GGG + chr2.4:57954092-57954111 MS.gene67877:CDS 40.0%
CATGGACCTTGAAGTAGTAA+TGG - chr2.4:57953646-57953665 None:intergenic 40.0%
CATTGAGCAATCCAAACTTG+TGG + chr2.4:57954358-57954377 MS.gene67877:CDS 40.0%
CCATTTCAACTTGAGACGAA+TGG + chr2.4:57953445-57953464 MS.gene67877:CDS 40.0%
CTTGCCATGGTTGCTAATAA+AGG - chr2.4:57953689-57953708 None:intergenic 40.0%
CTTTATTAGCAACCATGGCA+AGG + chr2.4:57953687-57953706 MS.gene67877:CDS 40.0%
GCAGTAAAGGAGCTGTTTAA+AGG + chr2.4:57953361-57953380 MS.gene67877:CDS 40.0%
GTCACTATTAAGGCCAACAT+AGG + chr2.4:57954090-57954109 MS.gene67877:CDS 40.0%
GTTTCAACTCTTGATGGAAC+AGG + chr2.4:57954477-57954496 MS.gene67877:CDS 40.0%
TGATTTCATGTTCCTTTGGC+AGG - chr2.4:57953420-57953439 None:intergenic 40.0%
TTGAGCAATCCAAACTTGTG+GGG + chr2.4:57954360-57954379 MS.gene67877:CDS 40.0%
! ATGCGATGAACAACTTTTGG+TGG + chr2.4:57954203-57954222 MS.gene67877:CDS 40.0%
! GTGGATATTTTATCACGGTG+AGG + chr2.4:57953806-57953825 MS.gene67877:CDS 40.0%
!! CCATTCGTCTCAAGTTGAAA+TGG - chr2.4:57953448-57953467 None:intergenic 40.0%
!! TGATATAAGAGGTGTTGTGG+CGG + chr2.4:57953339-57953358 MS.gene67877:CDS 40.0%
ATTCAACACCTTCCTGCCAA+AGG + chr2.4:57953405-57953424 MS.gene67877:CDS 45.0%
CATCGCATATAACGAGCCTT+AGG - chr2.4:57954191-57954210 None:intergenic 45.0%
CCTCCAATTCTTGCATACAC+CGG - chr2.4:57953566-57953585 None:intergenic 45.0%
CGGTGTATGCAAGAATTGGA+GGG + chr2.4:57953564-57953583 MS.gene67877:CDS 45.0%
CTACTTCAAGGTCCATGTAG+TGG + chr2.4:57953649-57953668 MS.gene67877:CDS 45.0%
CTCTCATATCCCCACAAGTT+TGG - chr2.4:57954372-57954391 None:intergenic 45.0%
GACGATCTCTTTCAGTTTGC+CGG + chr2.4:57953544-57953563 MS.gene67877:CDS 45.0%
TGGTTCTTCAGATGCCAAGA+AGG - chr2.4:57953626-57953645 None:intergenic 45.0%
TGTCAAAAGCAAGCAAGGCA+CGG - chr2.4:57953921-57953940 None:intergenic 45.0%
TTCATGTTCCTTTGGCAGGA+AGG - chr2.4:57953416-57953435 None:intergenic 45.0%
TTGCCGGTGTATGCAAGAAT+TGG + chr2.4:57953560-57953579 MS.gene67877:CDS 45.0%
AGGAACATGATCCCTTGCCA+TGG - chr2.4:57953702-57953721 None:intergenic 50.0%
CAAGCTGAGTACCCCTATGT+TGG - chr2.4:57954106-57954125 None:intergenic 50.0%
CATGATCGCTTGCCACTACA+TGG - chr2.4:57953664-57953683 None:intergenic 50.0%
CCGGTGTATGCAAGAATTGG+AGG + chr2.4:57953563-57953582 MS.gene67877:CDS 50.0%
CGATCGACTGGCATATCTTG+CGG + chr2.4:57953234-57953253 MS.gene67877:CDS 50.0%
CTCAAACCACTACGATCGAC+TGG + chr2.4:57953222-57953241 MS.gene67877:CDS 50.0%
GATATGCCAGTCGATCGTAG+TGG - chr2.4:57953231-57953250 None:intergenic 50.0%
! AGGTGTTGTGGCGGCAGTAA+AGG + chr2.4:57953348-57953367 MS.gene67877:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 57953211 57954566 57953211 ID=MS.gene67877
chr2.4 mRNA 57953211 57954566 57953211 ID=MS.gene67877.t1;Parent=MS.gene67877
chr2.4 exon 57953211 57954566 57953211 ID=MS.gene67877.t1.exon1;Parent=MS.gene67877.t1
chr2.4 CDS 57953211 57954566 57953211 ID=cds.MS.gene67877.t1;Parent=MS.gene67877.t1
Gene Sequence

>MS.gene67877

ATGAAGCTCTGCTCAAACCACTACGATCGACTGGCATATCTTGCGGCAGTCAAATTTGCATTTCAACATAACATGATGAAGTATGATCAATTCTTAAACGTTCTGATGAGTTTCAAGGATCATAGAATTGATATAAGAGGTGTTGTGGCGGCAGTAAAGGAGCTGTTTAAAGGGCATACAGATTTAATTTTGGGATTCAACACCTTCCTGCCAAAGGAACATGAAATCACACTTCCATTTCAACTTGAGACGAATGGAAAAACATTGAAGGTAGAAGAGAATTGTGAACTTCCTTCGGATGTGCTTGATATCATTTCCAAAACACTAGATTTTGACGATCTCTTTCAGTTTGCCGGTGTATGCAAGAATTGGAGGGTTTTTCATAAAATCTACTGGAGAAATTTCTTGGAATCCTTCTTGGCATCTGAAGAACCATTACTACTTCAAGGTCCATGTAGTGGCAAGCGATCATGTTCCTTTATTAGCAACCATGGCAAGGGATCATGTTCCTTTATTAGCATACCCAACCAAAAAGTTTATTGCTTGAAGATGATGGAATACTTCTTCAACCACTATAACTATGTTACATTTTCTAGTGGATATTTTATCACGGTGAGGGATGATGTTTATGTGCTAATCAACCCATTTACAAGAACAAAGAAGGTAATCGATACCCCTATCGGAATTAACAATTATAATTGTACTGACCGTGCCTTGCTTGCTTTTGACAAATACTCAGAAGAATTTATCTTGGTGATTTTATGTCTAAGTCGTTTGCATGTCTATCAATCTCGAAATTGTGGATGGGTTACTTATTCAACAATGAAAGATAAAGGGGTCGTGGTTGACTTTGTGATTTTCCATAATATAATATATGTTGTCACTATTAAGGCCAACATAGGGGTACTCAGCTTGAACTCTTCAAATATTAAATTTCTAAAACTGAATAGTACTCCCAATGAAATTTATTACTATAACCTAAGGCTCGTTATATGCGATGAACAACTTTTGGTGGTTAATTTAAGGCATAGGCAAATAAAGAATGTGTTCAAGATAGAAGGCTCAACCATGAATTATGTTAAAATGGAAACTTTGGGTGACATTGCACTATTTTATGTTGCTGATAAGTTCAGGGCAAATTGTTATGCATTGAGCAATCCAAACTTGTGGGGATATGAGAGCAACTCTGTGTACATCATCAATATGTTTTCTACAAGTTGTAGTGTGTATTCATGGGATGATAAAAAGTTGCAAAAATGCATTACGGTTTCAACTCTTGATGGAACAGGTTCTTTCATGTTTGATTGGTGTGTTAGACATCTACGATATGAAGTAGATTATTCTCTAGTTGAGTAG
Protein sequence

>MS.gene67877.t1

MKLCSNHYDRLAYLAAVKFAFQHNMMKYDQFLNVLMSFKDHRIDIRGVVAAVKELFKGHTDLILGFNTFLPKEHEITLPFQLETNGKTLKVEENCELPSDVLDIISKTLDFDDLFQFAGVCKNWRVFHKIYWRNFLESFLASEEPLLLQGPCSGKRSCSFISNHGKGSCSFISIPNQKVYCLKMMEYFFNHYNYVTFSSGYFITVRDDVYVLINPFTRTKKVIDTPIGINNYNCTDRALLAFDKYSEEFILVILCLSRLHVYQSRNCGWVTYSTMKDKGVVVDFVIFHNIIYVVTIKANIGVLSLNSSNIKFLKLNSTPNEIYYYNLRLVICDEQLLVVNLRHRQIKNVFKIEGSTMNYVKMETLGDIALFYVADKFRANCYALSNPNLWGYESNSVYIINMFSTSCSVYSWDDKKLQKCITVSTLDGTGSFMFDWCVRHLRYEVDYSLVE