AlfalfaGEDB | MS.gene68171

M. sativa cultivar XinJiangDaYe / MS.gene68171

KEGG: K19033
GO: GO:0005575 GO:0005840 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0110165
PFAM: PF17067.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68171.t1	KEH36378.1	95.1	81	3	1	1	81	1	80	3.00E-32	147.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68171.t1	P47909	57.7	71	27	1	11	81	4	71	4.5e-13	75.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68171.t1	A0A072V4Y3	95.1	81	3	1	1	81	1	80	2.1e-32	147.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene68171	0.817962	2.51E-52	-1.69E-46
MS.gene04917	MS.gene68171	0.819105	1.38E-52	-1.69E-46
MS.gene049255	MS.gene68171	0.802284	6.03E-49	-1.69E-46
MS.gene049382	MS.gene68171	0.80063	1.32E-48	-1.69E-46
MS.gene049703	MS.gene68171	-0.82132	4.30E-53	-1.69E-46
MS.gene049756	MS.gene68171	0.825409	4.76E-54	-1.69E-46
MS.gene04984	MS.gene68171	0.834948	2.23E-56	-1.69E-46
MS.gene049887	MS.gene68171	0.806768	7.01E-50	-1.69E-46
MS.gene050114	MS.gene68171	0.818133	2.30E-52	-1.69E-46
MS.gene050277	MS.gene68171	0.85353	2.25E-61	-1.69E-46
MS.gene050317	MS.gene68171	0.837961	3.82E-57	-1.69E-46
MS.gene050320	MS.gene68171	0.848292	6.73E-60	-1.69E-46
MS.gene050623	MS.gene68171	-0.800307	1.53E-48	-1.69E-46
MS.gene050838	MS.gene68171	0.833254	5.93E-56	-1.69E-46
MS.gene050992	MS.gene68171	0.800188	1.62E-48	-1.69E-46
MS.gene051063	MS.gene68171	0.830358	3.07E-55	-1.69E-46
MS.gene051243	MS.gene68171	0.820693	6.00E-53	-1.69E-46
MS.gene051245	MS.gene68171	0.872348	3.41E-67	-1.69E-46
MS.gene051328	MS.gene68171	0.810166	1.32E-50	-1.69E-46
MS.gene051633	MS.gene68171	0.836172	1.09E-56	-1.69E-46
MS.gene051746	MS.gene68171	0.841833	3.74E-58	-1.69E-46
MS.gene051748	MS.gene68171	0.853964	1.69E-61	-1.69E-46
MS.gene051756	MS.gene68171	0.824805	6.61E-54	-1.69E-46
MS.gene051757	MS.gene68171	0.828784	7.42E-55	-1.69E-46
MS.gene051903	MS.gene68171	0.843173	1.65E-58	-1.69E-46
MS.gene052286	MS.gene68171	0.825072	5.72E-54	-1.69E-46
MS.gene052302	MS.gene68171	0.827156	1.83E-54	-1.69E-46
MS.gene052376	MS.gene68171	0.821329	4.28E-53	-1.69E-46
MS.gene052397	MS.gene68171	0.80188	7.31E-49	-1.69E-46
MS.gene052434	MS.gene68171	0.812068	5.10E-51	-1.69E-46
MS.gene052856	MS.gene68171	0.82734	1.65E-54	-1.69E-46
MS.gene052947	MS.gene68171	0.801246	9.86E-49	-1.69E-46
MS.gene053058	MS.gene68171	0.81311	3.02E-51	-1.69E-46
MS.gene053109	MS.gene68171	0.859277	4.62E-63	-1.69E-46
MS.gene053381	MS.gene68171	0.808996	2.35E-50	-1.69E-46
MS.gene05344	MS.gene68171	-0.808972	2.38E-50	-1.69E-46
MS.gene054823	MS.gene68171	0.807072	6.04E-50	-1.69E-46
MS.gene055133	MS.gene68171	0.815744	7.90E-52	-1.69E-46
MS.gene055174	MS.gene68171	0.825854	3.74E-54	-1.69E-46
MS.gene055413	MS.gene68171	0.804109	2.53E-49	-1.69E-46
MS.gene055424	MS.gene68171	0.813343	2.69E-51	-1.69E-46
MS.gene055441	MS.gene68171	0.83905	2.00E-57	-1.69E-46
MS.gene056432	MS.gene68171	0.8435	1.35E-58	-1.69E-46
MS.gene056580	MS.gene68171	0.831904	1.28E-55	-1.69E-46
MS.gene056631	MS.gene68171	0.833069	6.59E-56	-1.69E-46
MS.gene056752	MS.gene68171	0.816594	5.10E-52	-1.69E-46
MS.gene056760	MS.gene68171	0.815995	6.95E-52	-1.69E-46
MS.gene056787	MS.gene68171	0.817291	3.56E-52	-1.69E-46
MS.gene057057	MS.gene68171	0.865277	6.63E-65	-1.69E-46
MS.gene057094	MS.gene68171	0.83675	7.79E-57	-1.69E-46
MS.gene057140	MS.gene68171	0.856197	3.79E-62	-1.69E-46
MS.gene057234	MS.gene68171	0.866152	3.51E-65	-1.69E-46
MS.gene057451	MS.gene68171	-0.817943	2.54E-52	-1.69E-46
MS.gene057452	MS.gene68171	-0.825313	5.02E-54	-1.69E-46
MS.gene057999	MS.gene68171	0.804342	2.26E-49	-1.69E-46
MS.gene058054	MS.gene68171	0.803207	3.89E-49	-1.69E-46
MS.gene058055	MS.gene68171	0.846756	1.78E-59	-1.69E-46
MS.gene058182	MS.gene68171	0.850472	1.66E-60	-1.69E-46
MS.gene058238	MS.gene68171	0.859417	4.20E-63	-1.69E-46
MS.gene058399	MS.gene68171	0.805386	1.37E-49	-1.69E-46
MS.gene058646	MS.gene68171	0.828325	9.57E-55	-1.69E-46
MS.gene058654	MS.gene68171	0.84471	6.39E-59	-1.69E-46
MS.gene058906	MS.gene68171	0.874582	6.04E-68	-1.69E-46
MS.gene059719	MS.gene68171	0.809356	1.97E-50	-1.69E-46
MS.gene059898	MS.gene68171	0.826011	3.43E-54	-1.69E-46
MS.gene06001	MS.gene68171	0.848587	5.57E-60	-1.69E-46
MS.gene060084	MS.gene68171	0.828583	8.29E-55	-1.69E-46
MS.gene06011	MS.gene68171	0.802599	5.20E-49	-1.69E-46
MS.gene060165	MS.gene68171	0.80452	2.08E-49	-1.69E-46
MS.gene060297	MS.gene68171	0.814206	1.73E-51	-1.69E-46
MS.gene060463	MS.gene68171	0.821528	3.85E-53	-1.69E-46
MS.gene060465	MS.gene68171	0.832579	8.72E-56	-1.69E-46
MS.gene060586	MS.gene68171	0.803145	4.01E-49	-1.69E-46
MS.gene060702	MS.gene68171	0.836975	6.83E-57	-1.69E-46
MS.gene060869	MS.gene68171	0.812594	3.92E-51	-1.69E-46
MS.gene060870	MS.gene68171	0.801839	7.45E-49	-1.69E-46
MS.gene061145	MS.gene68171	0.815743	7.91E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68171.t1	MTR_3g117570	93.902	82	4	1	1	82	1	81	2.05e-49	152
MS.gene68171.t1	MTR_3g117570	95.062	81	3	1	1	81	1	80	6.40e-49	151

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCACAAATACAGATTGTT+TGG	0.302653	3.1:-92501626	MS.gene68171:intron
ATAACTACTTCTCTTGGTTA+TGG	0.376607	3.1:-92501566	MS.gene68171:CDS
TCGATCATTTGTTCTCTCTT+CGG	0.404252	3.1:+92502299	None:intergenic
AAAAGATTCAAGGGCTCGTA+TGG	0.464506	3.1:-92502332	MS.gene68171:CDS
AAAGAAAGGGAAAAGATTCA+AGG	0.481880	3.1:-92502342	MS.gene68171:CDS
ACGCCTTGTGCTTCTTCTGC+CGG	0.494602	3.1:-92502410	MS.gene68171:CDS
GAGTGCATAACTACTTCTCT+TGG	0.501421	3.1:-92501572	MS.gene68171:CDS
GCTGCACGGTGGTTGATAAG+AGG	0.503001	3.1:-92502446	MS.gene68171:CDS
GATCGAACGAATCAAAGATA+AGG	0.533385	3.1:-92502282	MS.gene68171:CDS
GCTCCGATAGTGTGCGGACG+CGG	0.535798	3.1:-92502377	MS.gene68171:CDS
TCACCGCGTCCGCACACTAT+CGG	0.538358	3.1:+92502374	None:intergenic
TATCAACCACCGTGCAGCTA+CGG	0.541002	3.1:+92502451	None:intergenic
AAGATAAGGTTGAAGTTCCC+AGG	0.546325	3.1:-92502268	MS.gene68171:intron
AGGAATTTCGATAAAAGAAG+TGG	0.550885	3.1:-92501543	MS.gene68171:CDS
TATCGGAGCAGCTGTTACGC+CGG	0.576176	3.1:+92502391	None:intergenic
AAGAAAGGGAAAAGATTCAA+GGG	0.576778	3.1:-92502341	MS.gene68171:CDS
ACTACTTCTCTTGGTTATGG+AGG	0.586505	3.1:-92501563	MS.gene68171:CDS
AGCGGTGCACCGTAGCTGCA+CGG	0.586905	3.1:-92502460	MS.gene68171:CDS
GTTGATAAGAGGAGCGTCGT+CGG	0.589779	3.1:-92502435	MS.gene68171:CDS
AAAGATTCAAGGGCTCGTAT+GGG	0.635720	3.1:-92502331	MS.gene68171:CDS
GTGGCGCTAGTGTGATGCAG+CGG	0.656151	3.1:-92502478	MS.gene68171:CDS
AAAGTGTGTATGGCGAGTGG+TGG	0.695087	3.1:-92502497	None:intergenic
ACAGCTGCTCCGATAGTGTG+CGG	0.696198	3.1:-92502383	MS.gene68171:CDS
AGAAAAGTGTGTATGGCGAG+TGG	0.701894	3.1:-92502500	None:intergenic
ACGCCGGCAGAAGAAGCACA+AGG	0.711011	3.1:+92502407	None:intergenic
GGTGCACCGTAGCTGCACGG+TGG	0.772757	3.1:-92502457	MS.gene68171:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTAAAATCAAAATCCATA+AGG	-	chr3.1:92502104-92502123	MS.gene68171:intron	15.0%
!!	GAAATTGTTCATAGATATAT+AGG	-	chr3.1:92502289-92502308	MS.gene68171:CDS	20.0%
!!!	TATTTTGAGAATACTACTAA+TGG	-	chr3.1:92502191-92502210	MS.gene68171:intron	20.0%
!!!	TTTTCTGTTGAAATAGAAAA+TGG	+	chr3.1:92501861-92501880	None:intergenic	20.0%
!	GTGATAAAAAGACAAAGAAA+GGG	-	chr3.1:92501653-92501672	MS.gene68171:intron	25.0%
!	TAAATTTGTGACTTACAAGT+TGG	+	chr3.1:92502136-92502155	None:intergenic	25.0%
!	TTTGATGCATTATGTTATGT+TGG	-	chr3.1:92501887-92501906	MS.gene68171:intron	25.0%
!!	AAGTATTTGAAGTGAATTAG+AGG	+	chr3.1:92502074-92502093	None:intergenic	25.0%
!!	AGTATTTGAAGTGAATTAGA+GGG	+	chr3.1:92502073-92502092	None:intergenic	25.0%
!!	TCTTGCAAATTTTCCAAAAT+GGG	-	chr3.1:92502237-92502256	MS.gene68171:intron	25.0%
!!!	TCATTTCATTCCCATTTTTA+GGG	-	chr3.1:92501801-92501820	MS.gene68171:intron	25.0%
!!!	TTCATTTCATTCCCATTTTT+AGG	-	chr3.1:92501800-92501819	MS.gene68171:intron	25.0%
	AAACAATCTGTATTTGTGAG+TGG	+	chr3.1:92502383-92502402	None:intergenic	30.0%
	AAAGAAAGGGAAAAGATTCA+AGG	-	chr3.1:92501666-92501685	MS.gene68171:intron	30.0%
	AAATGCTAGTAATGTTTGTG+GGG	+	chr3.1:92502163-92502182	None:intergenic	30.0%
	AAGAAAGGGAAAAGATTCAA+GGG	-	chr3.1:92501667-92501686	MS.gene68171:intron	30.0%
	ACTCACAAATACAGATTGTT+TGG	-	chr3.1:92502382-92502401	MS.gene68171:CDS	30.0%
	AGAAATGCTAGTAATGTTTG+TGG	+	chr3.1:92502165-92502184	None:intergenic	30.0%
	AGGAATTTCGATAAAAGAAG+TGG	-	chr3.1:92502465-92502484	MS.gene68171:CDS	30.0%
	ATAACTACTTCTCTTGGTTA+TGG	-	chr3.1:92502442-92502461	MS.gene68171:CDS	30.0%
	GAAATGCTAGTAATGTTTGT+GGG	+	chr3.1:92502164-92502183	None:intergenic	30.0%
	GGTGATAAAAAGACAAAGAA+AGG	-	chr3.1:92501652-92501671	MS.gene68171:intron	30.0%
	TGAAATCAAATGAGCTTGAA+AGG	+	chr3.1:92501784-92501803	None:intergenic	30.0%
!	AAAATGGCAATGAAAGTACA+TGG	+	chr3.1:92501845-92501864	None:intergenic	30.0%
!	GTCTTGCAAATTTTCCAAAA+TGG	-	chr3.1:92502236-92502255	MS.gene68171:intron	30.0%
!!!	GACTTTATTTGTTTTGTGAG+TGG	+	chr3.1:92502219-92502238	None:intergenic	30.0%
	AATTCCACAGTGTTGTGTTT+GGG	-	chr3.1:92501921-92501940	MS.gene68171:intron	35.0%
	AGACGAGAAAACCCTAAAAA+TGG	+	chr3.1:92501815-92501834	None:intergenic	35.0%
	ATATAGGACACACAGATTCA+TGG	-	chr3.1:92502305-92502324	MS.gene68171:CDS	35.0%
	GACGAGAAAACCCTAAAAAT+GGG	+	chr3.1:92501814-92501833	None:intergenic	35.0%
	GATCGAACGAATCAAAGATA+AGG	-	chr3.1:92501726-92501745	MS.gene68171:intron	35.0%
	TAATTCCACAGTGTTGTGTT+TGG	-	chr3.1:92501920-92501939	MS.gene68171:intron	35.0%
	TCCTTACTCAAAGTTAGTGA+GGG	-	chr3.1:92502334-92502353	MS.gene68171:CDS	35.0%
	TCGATCATTTGTTCTCTCTT+CGG	+	chr3.1:92501712-92501731	None:intergenic	35.0%
!	GTAAAATAGCATGCCCATTT+TGG	+	chr3.1:92502253-92502272	None:intergenic	35.0%
!!	ACAAGTTGGTTTCAACCTTA+TGG	+	chr3.1:92502122-92502141	None:intergenic	35.0%
!!!	AAATGGGCATGCTATTTTAC+TGG	-	chr3.1:92502253-92502272	MS.gene68171:intron	35.0%
!!!	AATGGGCATGCTATTTTACT+GGG	-	chr3.1:92502254-92502273	MS.gene68171:intron	35.0%
	AAAACCCAAACACAACACTG+TGG	+	chr3.1:92501928-92501947	None:intergenic	40.0%
	AAAAGATTCAAGGGCTCGTA+TGG	-	chr3.1:92501676-92501695	MS.gene68171:intron	40.0%
	AAAGATTCAAGGGCTCGTAT+GGG	-	chr3.1:92501677-92501696	MS.gene68171:intron	40.0%
	AAGATAAGGTTGAAGTTCCC+AGG	-	chr3.1:92501740-92501759	MS.gene68171:intron	40.0%
	ACTACTTCTCTTGGTTATGG+AGG	-	chr3.1:92502445-92502464	MS.gene68171:CDS	40.0%
	CAAATGAGCTTGAAAGGAAG+AGG	+	chr3.1:92501778-92501797	None:intergenic	40.0%
	CCTTACTCAAAGTTAGTGAG+GGG	-	chr3.1:92502335-92502354	MS.gene68171:CDS	40.0%
	CTTACTCAAAGTTAGTGAGG+GGG	-	chr3.1:92502336-92502355	MS.gene68171:CDS	40.0%
	GAGTGCATAACTACTTCTCT+TGG	-	chr3.1:92502436-92502455	MS.gene68171:CDS	40.0%
	GTCCTTACTCAAAGTTAGTG+AGG	-	chr3.1:92502333-92502352	MS.gene68171:CDS	40.0%
	CCCCTCACTAACTTTGAGTA+AGG	+	chr3.1:92502338-92502357	None:intergenic	45.0%
	AAGTTCCCAGGTCTACTCCT+TGG	-	chr3.1:92501752-92501771	MS.gene68171:intron	50.0%
	AGCTTGAAAGGAAGAGGCCA+AGG	+	chr3.1:92501772-92501791	None:intergenic	50.0%
	GGGGTCATTCTTGGTTGATC+TGG	-	chr3.1:92502355-92502374	MS.gene68171:CDS	50.0%
	GTTAGTGAGGGGGTCATTCT+TGG	-	chr3.1:92502346-92502365	MS.gene68171:CDS	50.0%
	TATCAACCACCGTGCAGCTA+CGG	+	chr3.1:92501560-92501579	None:intergenic	50.0%
!!	GTTGATAAGAGGAGCGTCGT+CGG	-	chr3.1:92501573-92501592	MS.gene68171:CDS	50.0%
	AAGAGGCCAAGGAGTAGACC+TGG	+	chr3.1:92501761-92501780	None:intergenic	55.0%
	ACAGCTGCTCCGATAGTGTG+CGG	-	chr3.1:92501625-92501644	MS.gene68171:intron	55.0%
	ACGCCTTGTGCTTCTTCTGC+CGG	-	chr3.1:92501598-92501617	MS.gene68171:CDS	55.0%
	AGAGGCCAAGGAGTAGACCT+GGG	+	chr3.1:92501760-92501779	None:intergenic	55.0%
	TATCGGAGCAGCTGTTACGC+CGG	+	chr3.1:92501620-92501639	None:intergenic	55.0%
!!	GCTGCACGGTGGTTGATAAG+AGG	-	chr3.1:92501562-92501581	MS.gene68171:CDS	55.0%
	GTGGCGCTAGTGTGATGCAG+CGG	-	chr3.1:92501530-92501549	MS.gene68171:CDS	60.0%
	TCACCGCGTCCGCACACTAT+CGG	+	chr3.1:92501637-92501656	None:intergenic	60.0%
!	ACGCCGGCAGAAGAAGCACA+AGG	+	chr3.1:92501604-92501623	None:intergenic	60.0%
	AGCGGTGCACCGTAGCTGCA+CGG	-	chr3.1:92501548-92501567	MS.gene68171:CDS	65.0%
	GCTCCGATAGTGTGCGGACG+CGG	-	chr3.1:92501631-92501650	MS.gene68171:intron	65.0%
	GGTGCACCGTAGCTGCACGG+TGG	-	chr3.1:92501551-92501570	MS.gene68171:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	92501520	92502510	92501520	ID=MS.gene68171
chr3.1	mRNA	92501520	92502510	92501520	ID=MS.gene68171.t1;Parent=MS.gene68171
chr3.1	exon	92502269	92502510	92502269	ID=MS.gene68171.t1.exon1;Parent=MS.gene68171.t1
chr3.1	CDS	92502269	92502510	92502269	ID=cds.MS.gene68171.t1;Parent=MS.gene68171.t1
chr3.1	exon	92501520	92501634	92501520	ID=MS.gene68171.t1.exon2;Parent=MS.gene68171.t1
chr3.1	CDS	92501520	92501634	92501520	ID=cds.MS.gene68171.t1;Parent=MS.gene68171.t1

Gene Sequence

>MS.gene68171

ATGGCGAGTGGTGGCGCTAGTGTGATGCAGCGGTGCACCGTAGCTGCACGGTGGTTGATAAGAGGAGCGTCGTCGGAGACGCCTTGTGCTTCTTCTGCCGGCGTAACAGCTGCTCCGATAGTGTGCGGACGCGGTGATAAAAAGACAAAGAAAGGGAAAAGATTCAAGGGCTCGTATGGGAAATCTCGTCCGAAGAGAGAACAAATGATCGAACGAATCAAAGATAAGGTTGAAGTTCCCAGGTCTACTCCTTGGCCTCTTCCTTTCAAGCTCATTTGATTTCATTTCATTCCCATTTTTAGGGTTTTCTCGTCTGAACAATCCATGTACTTTCATTGCCATTTTCTATTTCAACAGAAAATTTTATTTTGATGCATTATGTTATGTTGGTTGCTCATTGTAATTCCACAGTGTTGTGTTTGGGTTTTATCAATCATTACAAACAAATTAATAAATTTGCTGTGCAATATTGTTGTTTATGCGTATAGATTAATCATTTCACTCTCACAGACACTTGTATAGAAAAATATCGTAAATATGAAAAGTAAAGCCCTCTAATTCACTTCAAATACTTAATCAGATTGATTTAAAATCAAAATCCATAAGGTTGAAACCAACTTGTAAGTCACAAATTTAAGAGCCCCACAAACATTACTAGCATTTCTAGAAAATATTTTGAGAATACTACTAATGGAACCACTCACAAAACAAATAAAGTCTTGCAAATTTTCCAAAATGGGCATGCTATTTTACTGGGATTCGTTTTTTAGAAATTGTTCATAGATATATAGGACACACAGATTCATGGACACAGTCCTTACTCAAAGTTAGTGAGGGGGTCATTCTTGGTTGATCTGGTTCCACTCACAAATACAGATTGTTTGGAATGTGCACTGCAGCATTTGCAGAGAAATTAGAGTGCATAACTACTTCTCTTGGTTATGGAGGAATTTCGATAAAAGAAGTGGACAGTTCTTGTATAAAATGCTAA

Protein sequence

>MS.gene68171.t1

MASGGASVMQRCTVAARWLIRGASSETPCASSAGVTAAPIVCGRGDKKTKKGKRFKGSYGKSRPKREQMIERIKDKVEVPRLFGMCTAAFAEKLECITTSLGYGGISIKEVDSSCIKC