AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99313


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATGAAGGATTGTTTCTA+AGG 0.251354 6.3:+10307783 None:intergenic
TGTATCGAAGTTGGGCTTGC+TGG 0.327795 6.3:+10307807 None:intergenic
AGAGTCGGTGTAAGAGAGAT+TGG 0.343423 6.3:+10306832 None:intergenic
TCCAACGGTGTCCACAGTTT+GGG 0.348103 6.3:-10307230 MS.gene99313:CDS
TTCCAACGGTGTCCACAGTT+TGG 0.352249 6.3:-10307231 MS.gene99313:CDS
TTGAACGCAGCGAACGGCTT+TGG 0.355771 6.3:-10307127 MS.gene99313:CDS
CCTTCATTTCTTCGATGATA+AGG 0.358076 6.3:-10307770 MS.gene99313:intron
TCTGTTCTCTGTCTTCCTTA+AGG 0.368606 6.3:-10306960 MS.gene99313:CDS
GAGGGAAGAGAGTGTGAGTT+AGG 0.376366 6.3:+10306870 None:intergenic
TGAGCGCACTCAAGTGTTGC+AGG 0.377539 6.3:+10307074 None:intergenic
TGGTACTCATCTTGTCTTGT+TGG 0.380839 6.3:-10306903 MS.gene99313:CDS
TGAATCTCGAATGCGCCGTT+TGG 0.398019 6.3:-10307908 None:intergenic
CCTCGCCTCTGGCAGTTGCC+TGG 0.401298 6.3:-10307176 MS.gene99313:CDS
ACATTCACGGCAAAACTTCA+TGG 0.409173 6.3:-10306923 MS.gene99313:CDS
AGAAGTTATCAGCTGCCTTC+TGG 0.416563 6.3:-10307017 MS.gene99313:CDS
TCTAAGGGTGTATCGAAGTT+GGG 0.431737 6.3:+10307799 None:intergenic
CGTCATTCCGTCCTCGCCTC+TGG 0.431810 6.3:-10307187 MS.gene99313:CDS
TAACAACATTCTGTACCTTA+AGG 0.438260 6.3:+10306945 None:intergenic
TTCTAAGGGTGTATCGAAGT+TGG 0.438874 6.3:+10307798 None:intergenic
CCTTCTGGTGATCGCAATTA+CGG 0.443863 6.3:-10307002 MS.gene99313:CDS
AGATTGGAAAGGAGAGAAGT+GGG 0.444912 6.3:+10306848 None:intergenic
GTATCGAAGTTGGGCTTGCT+GGG 0.471039 6.3:+10307808 None:intergenic
CCTTATCATCGAAGAAATGA+AGG 0.473594 6.3:+10307770 None:intergenic
CGCTGCGTTCAATGCAATCA+TGG 0.490943 6.3:+10307138 None:intergenic
CGAAAATAACTGTTCTTCGA+TGG 0.507553 6.3:-10307861 MS.gene99313:CDS
CGCAATTACGGCAAGCGTAG+AGG 0.511532 6.3:-10306990 MS.gene99313:CDS
GAGATTGGAAAGGAGAGAAG+TGG 0.517620 6.3:+10306847 None:intergenic
ACAGAATGTTGTTACATTCA+CGG 0.518581 6.3:-10306936 MS.gene99313:CDS
GAGCACGTGCTTCAGTTTGA+CGG 0.521354 6.3:+10307830 None:intergenic
GCAACTGCCAGAGGCGAGGA+CGG 0.521541 6.3:+10307180 None:intergenic
TGGAAAGGAGAGAAGTGGGA+GGG 0.552626 6.3:+10306852 None:intergenic
GCTTTGGTGCGAGGAAGTGT+TGG 0.554939 6.3:-10307111 MS.gene99313:CDS
ACCCAAACTGTGGACACCGT+TGG 0.558999 6.3:+10307229 None:intergenic
CAGCGAACGGCTTTGGTGCG+AGG 0.559451 6.3:-10307120 MS.gene99313:CDS
CCAGGCAACTGCCAGAGGCG+AGG 0.567483 6.3:+10307176 None:intergenic
GAAAGTAGTTTAGTAAGAGT+CGG 0.567570 6.3:+10306817 None:intergenic
TTGGAAAGGAGAGAAGTGGG+AGG 0.571142 6.3:+10306851 None:intergenic
AAACAACGGATATCTCCAAA+CGG 0.577476 6.3:+10307893 None:intergenic
AAATGAAGGATTGTTTCTAA+GGG 0.580437 6.3:+10307784 None:intergenic
CGGTGTAAGAGAGATTGGAA+AGG 0.583026 6.3:+10306837 None:intergenic
ATTGCATTGAACGCAGCGAA+CGG 0.596659 6.3:-10307133 MS.gene99313:CDS
GTTTGCTGCAACCCAAACTG+TGG 0.602356 6.3:+10307219 None:intergenic
CCGTAATTGCGATCACCAGA+AGG 0.604582 6.3:+10307002 None:intergenic
TTTCGGATAAGGACAAACAA+CGG 0.634830 6.3:+10307879 None:intergenic
ACGAAACTCAGTCTTTCCAA+CGG 0.689569 6.3:-10307245 MS.gene99313:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAGATAGTATTTGTTTAA+AGG + chr6.3:10307150-10307169 None:intergenic 15.0%
!! TATTAAAAATCAAGTGATGT+GGG + chr6.3:10307369-10307388 None:intergenic 20.0%
!! TTATTAAAAATCAAGTGATG+TGG + chr6.3:10307370-10307389 None:intergenic 20.0%
!!! AATTATAGCCTTAAAAACAT+AGG - chr6.3:10307012-10307031 MS.gene99313:CDS 20.0%
! ATTAAAAATCAAGTGATGTG+GGG + chr6.3:10307368-10307387 None:intergenic 25.0%
!!! AAATGAAGGATTGTTTCTAA+GGG + chr6.3:10306943-10306962 None:intergenic 25.0%
ACAGAATGTTGTTACATTCA+CGG - chr6.3:10307788-10307807 MS.gene99313:CDS 30.0%
GTTGTCGATCAAATGATTAA+TGG + chr6.3:10307290-10307309 None:intergenic 30.0%
TAACAAACCAAGGAAATTCA+GGG - chr6.3:10307222-10307241 MS.gene99313:CDS 30.0%
TAACAACATTCTGTACCTTA+AGG + chr6.3:10307782-10307801 None:intergenic 30.0%
TCTCTAATCTGTGTTAAAAC+AGG - chr6.3:10307338-10307357 MS.gene99313:intron 30.0%
TTAACAAACCAAGGAAATTC+AGG - chr6.3:10307221-10307240 MS.gene99313:CDS 30.0%
!! ACTGTTAGCCTATGTTTTTA+AGG + chr6.3:10307023-10307042 None:intergenic 30.0%
!! AGAACAGTTATTTTCGGATA+AGG + chr6.3:10306859-10306878 None:intergenic 30.0%
!! CATCGAAGAACAGTTATTTT+CGG + chr6.3:10306865-10306884 None:intergenic 30.0%
!! GAAATGAAGGATTGTTTCTA+AGG + chr6.3:10306944-10306963 None:intergenic 30.0%
AAACAACGGATATCTCCAAA+CGG + chr6.3:10306834-10306853 None:intergenic 35.0%
CCTTATCATCGAAGAAATGA+AGG + chr6.3:10306957-10306976 None:intergenic 35.0%
CCTTCATTTCTTCGATGATA+AGG - chr6.3:10306954-10306973 MS.gene99313:CDS 35.0%
CGAAAATAACTGTTCTTCGA+TGG - chr6.3:10306863-10306882 MS.gene99313:CDS 35.0%
CTTAAAGCGCAACACAAAAA+TGG - chr6.3:10307165-10307184 MS.gene99313:CDS 35.0%
GAGGAAATGTGGAATCAAAA+TGG + chr6.3:10307191-10307210 None:intergenic 35.0%
GTAGTGTTAAATGCAGTCAA+AGG + chr6.3:10306988-10307007 None:intergenic 35.0%
TCGACAACTACAGTTAAAAC+AGG - chr6.3:10307302-10307321 MS.gene99313:intron 35.0%
TTTCGGATAAGGACAAACAA+CGG + chr6.3:10306848-10306867 None:intergenic 35.0%
! AAGGTTCAACTCAACTTTTG+GGG + chr6.3:10307446-10307465 None:intergenic 35.0%
! CAAGGTTCAACTCAACTTTT+GGG + chr6.3:10307447-10307466 None:intergenic 35.0%
! TCAAGGTTCAACTCAACTTT+TGG + chr6.3:10307448-10307467 None:intergenic 35.0%
! TGGCGTGCTGAAATTTTATA+TGG + chr6.3:10307252-10307271 None:intergenic 35.0%
!! AGTTGAGTTGAACCTTGATT+TGG - chr6.3:10307450-10307469 MS.gene99313:intron 35.0%
!! GTTGAGTTGAACCTTGATTT+GGG - chr6.3:10307451-10307470 MS.gene99313:intron 35.0%
!! TCCTTGGTTTGTTAACTTGA+GGG + chr6.3:10307216-10307235 None:intergenic 35.0%
!! TTCCTTGGTTTGTTAACTTG+AGG + chr6.3:10307217-10307236 None:intergenic 35.0%
ACATTCACGGCAAAACTTCA+TGG - chr6.3:10307801-10307820 MS.gene99313:CDS 40.0%
ACGAAACTCAGTCTTTCCAA+CGG - chr6.3:10307479-10307498 MS.gene99313:intron 40.0%
AGATTGGAAAGGAGAGAAGT+GGG + chr6.3:10307879-10307898 None:intergenic 40.0%
CTCATTATGTTCTCCACTCT+AGG + chr6.3:10307412-10307431 None:intergenic 40.0%
CTTTCTCTTCTATCCTAGAG+TGG - chr6.3:10307396-10307415 MS.gene99313:intron 40.0%
TCCCTCAAGTTAACAAACCA+AGG - chr6.3:10307212-10307231 MS.gene99313:CDS 40.0%
TCTGTTCTCTGTCTTCCTTA+AGG - chr6.3:10307764-10307783 MS.gene99313:intron 40.0%
TGGTACTCATCTTGTCTTGT+TGG - chr6.3:10307821-10307840 MS.gene99313:CDS 40.0%
! AGGTTCAACTCAACTTTTGG+GGG + chr6.3:10307445-10307464 None:intergenic 40.0%
! GATTAATGGACAATCCTTGC+AGG + chr6.3:10307276-10307295 None:intergenic 40.0%
! GTTTGTTAACTTGAGGGATG+AGG + chr6.3:10307210-10307229 None:intergenic 40.0%
! TCTAAGGGTGTATCGAAGTT+GGG + chr6.3:10306928-10306947 None:intergenic 40.0%
! TTCTAAGGGTGTATCGAAGT+TGG + chr6.3:10306929-10306948 None:intergenic 40.0%
AATGGACAATCCTTGCAGGT+TGG + chr6.3:10307272-10307291 None:intergenic 45.0%
ACTTGAGGGATGAGGAAATG+TGG + chr6.3:10307202-10307221 None:intergenic 45.0%
AGAAGTTATCAGCTGCCTTC+TGG - chr6.3:10307707-10307726 MS.gene99313:intron 45.0%
AGAGTCGGTGTAAGAGAGAT+TGG + chr6.3:10307895-10307914 None:intergenic 45.0%
ATTGCATTGAACGCAGCGAA+CGG - chr6.3:10307591-10307610 MS.gene99313:intron 45.0%
CCTTCTGGTGATCGCAATTA+CGG - chr6.3:10307722-10307741 MS.gene99313:intron 45.0%
CGGTGTAAGAGAGATTGGAA+AGG + chr6.3:10307890-10307909 None:intergenic 45.0%
GAGATTGGAAAGGAGAGAAG+TGG + chr6.3:10307880-10307899 None:intergenic 45.0%
TGGATCACCCTGAATTTCCT+TGG + chr6.3:10307232-10307251 None:intergenic 45.0%
! TGGTGAAGCTGTGTTTTTCC+AGG + chr6.3:10307569-10307588 None:intergenic 45.0%
!! AGTTGAACCTTGATTTGGGC+AGG - chr6.3:10307455-10307474 MS.gene99313:intron 45.0%
CCGTAATTGCGATCACCAGA+AGG + chr6.3:10307725-10307744 None:intergenic 50.0%
CGCTGCGTTCAATGCAATCA+TGG + chr6.3:10307589-10307608 None:intergenic 50.0%
GAGCACGTGCTTCAGTTTGA+CGG + chr6.3:10306897-10306916 None:intergenic 50.0%
TCCAACGGTGTCCACAGTTT+GGG - chr6.3:10307494-10307513 MS.gene99313:intron 50.0%
TGGAAAGGAGAGAAGTGGGA+GGG + chr6.3:10307875-10307894 None:intergenic 50.0%
TGTATCGAAGTTGGGCTTGC+TGG + chr6.3:10306920-10306939 None:intergenic 50.0%
TTCCAACGGTGTCCACAGTT+TGG - chr6.3:10307493-10307512 MS.gene99313:intron 50.0%
TTGGAAAGGAGAGAAGTGGG+AGG + chr6.3:10307876-10307895 None:intergenic 50.0%
TTTCGTGCCTGCCCAAATCA+AGG + chr6.3:10307465-10307484 None:intergenic 50.0%
! GAGGGAAGAGAGTGTGAGTT+AGG + chr6.3:10307857-10307876 None:intergenic 50.0%
! GTATCGAAGTTGGGCTTGCT+GGG + chr6.3:10306919-10306938 None:intergenic 50.0%
! GTTTGCTGCAACCCAAACTG+TGG + chr6.3:10307508-10307527 None:intergenic 50.0%
! TTTTTCCAGGCAACTGCCAG+AGG + chr6.3:10307556-10307575 None:intergenic 50.0%
ACCCAAACTGTGGACACCGT+TGG + chr6.3:10307498-10307517 None:intergenic 55.0%
CGCAATTACGGCAAGCGTAG+AGG - chr6.3:10307734-10307753 MS.gene99313:intron 55.0%
TTGAACGCAGCGAACGGCTT+TGG - chr6.3:10307597-10307616 MS.gene99313:intron 55.0%
TTTCAGCACGCCAACCTGCA+AGG - chr6.3:10307259-10307278 MS.gene99313:intron 55.0%
! GCTTTGGTGCGAGGAAGTGT+TGG - chr6.3:10307613-10307632 MS.gene99313:intron 55.0%
! TGAGCGCACTCAAGTGTTGC+AGG + chr6.3:10307653-10307672 None:intergenic 55.0%
CGTCATTCCGTCCTCGCCTC+TGG - chr6.3:10307537-10307556 MS.gene99313:intron 65.0%
GCAACTGCCAGAGGCGAGGA+CGG + chr6.3:10307547-10307566 None:intergenic 65.0%
! CAGCGAACGGCTTTGGTGCG+AGG - chr6.3:10307604-10307623 MS.gene99313:intron 65.0%
CCAGGCAACTGCCAGAGGCG+AGG + chr6.3:10307551-10307570 None:intergenic 70.0%
CCTCGCCTCTGGCAGTTGCC+TGG - chr6.3:10307548-10307567 MS.gene99313:intron 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 10306826 10307920 10306826 ID=MS.gene99313
chr6.3 mRNA 10306826 10307920 10306826 ID=MS.gene99313.t1;Parent=MS.gene99313
chr6.3 exon 10307771 10307920 10307771 ID=MS.gene99313.t1.exon1;Parent=MS.gene99313.t1
chr6.3 CDS 10307771 10307920 10307771 ID=cds.MS.gene99313.t1;Parent=MS.gene99313.t1
chr6.3 exon 10306826 10307269 10306826 ID=MS.gene99313.t1.exon2;Parent=MS.gene99313.t1
chr6.3 CDS 10306826 10307269 10306826 ID=cds.MS.gene99313.t1;Parent=MS.gene99313.t1
Gene Sequence

>MS.gene99313

ATGCGCCGTTTGGAGATATCCGTTGTTTGTCCTTATCCGAAAATAACTGTTCTTCGATGGTTCTTCATCCGTCAAACTGAAGCACGTGCTCCCAGCAAGCCCAACTTCGATACACCCTTAGAAACAATCCTTCATTTCTTCGATGATAAGGTCAACTCTCCTTTGACTGCATTTAACACTACATTAAATTATAGCCTTAAAAACATAGGCTAACAGTTACATGAATTTGTCTTACAATAACTTAGAATTTTTCTGTCATCTTTCGTTGTGTATATTTTCGATTCTTACTAAACTTTTTTTTTTTAAGTGTTCAACAAGAAACCTTTAAACAAATACTATCTTAAAGCGCAACACAAAAATGGCCATTTTGATTCCACATTTCCTCATCCCTCAAGTTAACAAACCAAGGAAATTCAGGGTGATCCATATAAAATTTCAGCACGCCAACCTGCAAGGATTGTCCATTAATCATTTGATCGACAACTACAGTTAAAACAGGTATCACACATAATTCTCTAATCTGTGTTAAAACAGGTCTTCCCCACATCACTTGATTTTTAATAACTACAACTTTCTCTTCTATCCTAGAGTGGAGAACATAATGAGTTATAGATTTCCCCCAAAAGTTGAGTTGAACCTTGATTTGGGCAGGCACGAAACTCAGTCTTTCCAACGGTGTCCACAGTTTGGGTTGCAGCAAACGAAGAAAAGCGTCATTCCGTCCTCGCCTCTGGCAGTTGCCTGGAAAAACACAGCTTCACCATGATTGCATTGAACGCAGCGAACGGCTTTGGTGCGAGGAAGTGTTGGATCTGCAGCTACATCCTGCAACACTTGAGTGCGCTCAGCGACAGAGTGATGAATCTCATTTCTGTAGACACAGAAGTTATCAGCTGCCTTCTGGTGATCGCAATTACGGCAAGCGTAGAGGAGAATCTTCTGTTCTCTGTCTTCCTTAAGGTACAGAATGTTGTTACATTCACGGCAAAACTTCATGGTACTCATCTTGTCTTGTTGGAAGAAGAAGACCTAACTCACACTCTCTTCCCTCCCACTTCTCTCCTTTCCAATCTCTCTTACACCGACTCTTACTAA

Protein sequence

>MS.gene99313.t1

MRRLEISVVCPYPKITVLRWFFIRQTEARAPSKPNFDTPLETILHFFDDKARNSVFPTVSTVWVAANEEKRHSVLASGSCLEKHSFTMIALNAANGFGARKCWICSYILQHLSALSDRVMNLISVDTEVISCLLVIAITASVEENLLFSVFLKVQNVVTFTAKLHGTHLVLLEEEDLTHTLFPPTSLLSNLSYTDSY