Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68810.t1 | XP_003626648.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 6.30E-29 | 136 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68810.t1 | Q9C9Z5 | 77.4 | 62 | 14 | 0 | 1 | 62 | 1 | 62 | 2.0e-23 | 109.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68810.t1 | Q1STQ6 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 4.6e-29 | 136.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene68810 | 0.852717 | 3.84E-61 | -1.69E-46 |
| MS.gene049341 | MS.gene68810 | 0.848372 | 6.39E-60 | -1.69E-46 |
| MS.gene049382 | MS.gene68810 | 0.807229 | 5.60E-50 | -1.69E-46 |
| MS.gene049703 | MS.gene68810 | -0.802596 | 5.20E-49 | -1.69E-46 |
| MS.gene049756 | MS.gene68810 | 0.831269 | 1.84E-55 | -1.69E-46 |
| MS.gene049887 | MS.gene68810 | 0.807444 | 5.04E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene68810 | 0.896794 | 2.62E-76 | -1.69E-46 |
| MS.gene050320 | MS.gene68810 | 0.810121 | 1.35E-50 | -1.69E-46 |
| MS.gene050437 | MS.gene68810 | 0.859666 | 3.53E-63 | -1.69E-46 |
| MS.gene050689 | MS.gene68810 | 0.820495 | 6.66E-53 | -1.69E-46 |
| MS.gene050752 | MS.gene68810 | 0.81553 | 8.82E-52 | -1.69E-46 |
| MS.gene050815 | MS.gene68810 | 0.84918 | 3.82E-60 | -1.69E-46 |
| MS.gene050838 | MS.gene68810 | 0.841587 | 4.34E-58 | -1.69E-46 |
| MS.gene051063 | MS.gene68810 | 0.847215 | 1.33E-59 | -1.69E-46 |
| MS.gene051241 | MS.gene68810 | 0.828877 | 7.04E-55 | -1.69E-46 |
| MS.gene051243 | MS.gene68810 | 0.837345 | 5.49E-57 | -1.69E-46 |
| MS.gene051245 | MS.gene68810 | 0.865273 | 6.65E-65 | -1.69E-46 |
| MS.gene051328 | MS.gene68810 | 0.851622 | 7.87E-61 | -1.69E-46 |
| MS.gene05134 | MS.gene68810 | 0.840389 | 8.96E-58 | -1.69E-46 |
| MS.gene051451 | MS.gene68810 | 0.829632 | 4.62E-55 | -1.69E-46 |
| MS.gene051633 | MS.gene68810 | 0.836941 | 6.97E-57 | -1.69E-46 |
| MS.gene051746 | MS.gene68810 | 0.852558 | 4.27E-61 | -1.69E-46 |
| MS.gene051748 | MS.gene68810 | 0.84829 | 6.73E-60 | -1.69E-46 |
| MS.gene051756 | MS.gene68810 | 0.831971 | 1.23E-55 | -1.69E-46 |
| MS.gene051757 | MS.gene68810 | 0.850865 | 1.29E-60 | -1.69E-46 |
| MS.gene051789 | MS.gene68810 | 0.80661 | 7.57E-50 | -1.69E-46 |
| MS.gene051850 | MS.gene68810 | 0.820808 | 5.64E-53 | -1.69E-46 |
| MS.gene051903 | MS.gene68810 | 0.857315 | 1.78E-62 | -1.69E-46 |
| MS.gene052133 | MS.gene68810 | 0.813691 | 2.25E-51 | -1.69E-46 |
| MS.gene052202 | MS.gene68810 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene052286 | MS.gene68810 | 0.855579 | 5.74E-62 | -1.69E-46 |
| MS.gene052302 | MS.gene68810 | 0.821131 | 4.75E-53 | -1.69E-46 |
| MS.gene052376 | MS.gene68810 | 0.814203 | 1.74E-51 | -1.69E-46 |
| MS.gene052856 | MS.gene68810 | 0.805488 | 1.30E-49 | -1.69E-46 |
| MS.gene052879 | MS.gene68810 | 0.808903 | 2.46E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene68810 | 0.846466 | 2.13E-59 | -1.69E-46 |
| MS.gene053109 | MS.gene68810 | 0.861008 | 1.39E-63 | -1.69E-46 |
| MS.gene053216 | MS.gene68810 | 0.803312 | 3.70E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene68810 | 0.80392 | 2.77E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene68810 | 0.808322 | 3.28E-50 | -1.69E-46 |
| MS.gene055133 | MS.gene68810 | 0.824029 | 1.01E-53 | -1.69E-46 |
| MS.gene055162 | MS.gene68810 | 0.872988 | 2.08E-67 | -1.69E-46 |
| MS.gene055424 | MS.gene68810 | 0.801546 | 8.56E-49 | -1.69E-46 |
| MS.gene055441 | MS.gene68810 | 0.808153 | 3.56E-50 | -1.69E-46 |
| MS.gene055591 | MS.gene68810 | -0.802207 | 6.26E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene68810 | -0.816505 | 5.34E-52 | -1.69E-46 |
| MS.gene056432 | MS.gene68810 | 0.847225 | 1.32E-59 | -1.69E-46 |
| MS.gene056580 | MS.gene68810 | 0.830766 | 2.44E-55 | -1.69E-46 |
| MS.gene056601 | MS.gene68810 | 0.86017 | 2.49E-63 | -1.69E-46 |
| MS.gene056631 | MS.gene68810 | 0.871441 | 6.82E-67 | -1.69E-46 |
| MS.gene056760 | MS.gene68810 | 0.857093 | 2.06E-62 | -1.69E-46 |
| MS.gene057057 | MS.gene68810 | 0.881059 | 3.29E-70 | -1.69E-46 |
| MS.gene057094 | MS.gene68810 | 0.819517 | 1.11E-52 | -1.69E-46 |
| MS.gene057140 | MS.gene68810 | 0.856922 | 2.32E-62 | -1.69E-46 |
| MS.gene057234 | MS.gene68810 | 0.89732 | 1.58E-76 | -1.69E-46 |
| MS.gene057705 | MS.gene68810 | 0.82051 | 6.60E-53 | -1.69E-46 |
| MS.gene05795 | MS.gene68810 | 0.804075 | 2.57E-49 | -1.69E-46 |
| MS.gene058054 | MS.gene68810 | 0.819219 | 1.30E-52 | -1.69E-46 |
| MS.gene058055 | MS.gene68810 | 0.87043 | 1.47E-66 | -1.69E-46 |
| MS.gene058084 | MS.gene68810 | 0.807052 | 6.10E-50 | -1.69E-46 |
| MS.gene05809 | MS.gene68810 | 0.807117 | 5.91E-50 | -1.69E-46 |
| MS.gene058182 | MS.gene68810 | 0.823034 | 1.72E-53 | -1.69E-46 |
| MS.gene058238 | MS.gene68810 | 0.859992 | 2.82E-63 | -1.69E-46 |
| MS.gene058646 | MS.gene68810 | 0.811772 | 5.92E-51 | -1.69E-46 |
| MS.gene058654 | MS.gene68810 | 0.820614 | 6.25E-53 | -1.69E-46 |
| MS.gene058906 | MS.gene68810 | 0.910275 | 2.25E-82 | -1.69E-46 |
| MS.gene059898 | MS.gene68810 | 0.875799 | 2.32E-68 | -1.69E-46 |
| MS.gene06001 | MS.gene68810 | 0.867238 | 1.59E-65 | -1.69E-46 |
| MS.gene060165 | MS.gene68810 | 0.803774 | 2.97E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene68810 | 0.800857 | 1.18E-48 | -1.69E-46 |
| MS.gene060463 | MS.gene68810 | 0.815064 | 1.12E-51 | -1.69E-46 |
| MS.gene060465 | MS.gene68810 | 0.81791 | 2.58E-52 | -1.69E-46 |
| MS.gene060702 | MS.gene68810 | 0.833772 | 4.40E-56 | -1.69E-46 |
| MS.gene061145 | MS.gene68810 | 0.841895 | 3.60E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68810.t1 | MTR_8g005360 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 1.66e-43 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68810.t1 | AT3G08610 | 75.000 | 64 | 16 | 0 | 1 | 64 | 1 | 64 | 2.40e-33 | 108 |
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGTACCTGTTTGTTAAT+TGG | 0.273208 | 8.4:-81671052 | None:intergenic |
| TGCTGGAATGCTTTGTGTTA+TGG | 0.308874 | 8.4:+81670815 | MS.gene68810:CDS |
| GCTGGAATGCTTTGTGTTAT+GGG | 0.333642 | 8.4:+81670816 | MS.gene68810:CDS |
| TTACCTTTGGGAATAATTGC+TGG | 0.341296 | 8.4:+81670798 | MS.gene68810:CDS |
| GTTGGAAGCTATGTTACCTT+TGG | 0.352532 | 8.4:+81670785 | MS.gene68810:CDS |
| TTGGAAGCTATGTTACCTTT+GGG | 0.406774 | 8.4:+81670786 | MS.gene68810:CDS |
| CTTCTTGTCCCTACGTTCCA+TGG | 0.446698 | 8.4:-81671007 | None:intergenic |
| AAATCATTGCCGATGTGCTT+AGG | 0.473520 | 8.4:-81670973 | None:intergenic |
| AGCACATCGGCAATGATTTG+TGG | 0.486432 | 8.4:+81670977 | MS.gene68810:CDS |
| AGAGATGGCGTTGCATTGGT+TGG | 0.487344 | 8.4:+81670767 | None:intergenic |
| GCACATCGGCAATGATTTGT+GGG | 0.508503 | 8.4:+81670978 | MS.gene68810:CDS |
| AGAGAGAGATGGCGTTGCAT+TGG | 0.509492 | 8.4:+81670763 | None:intergenic |
| CGGCAATGATTTGTGGGACG+TGG | 0.531999 | 8.4:+81670984 | MS.gene68810:CDS |
| TGATTTGTGGGACGTGGCCA+TGG | 0.553811 | 8.4:+81670990 | MS.gene68810:CDS |
| GGACGTGGCCATGGAACGTA+GGG | 0.570234 | 8.4:+81670999 | MS.gene68810:CDS |
| ATTCCAGCAATTATTCCCAA+AGG | 0.601922 | 8.4:-81670801 | None:intergenic |
| TGTGGTTAGCCTAAGCACAT+CGG | 0.628887 | 8.4:+81670964 | MS.gene68810:intron |
| CTCGTCCGTGAGCAGCTCTG+TGG | 0.632697 | 8.4:-81670857 | None:intergenic |
| TACATCCACAGAGCTGCTCA+CGG | 0.640107 | 8.4:+81670852 | MS.gene68810:CDS |
| GGGACGTGGCCATGGAACGT+AGG | 0.664354 | 8.4:+81670998 | MS.gene68810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAAAATTGAATTGAAT+TGG | + | chr8.4:81670932-81670951 | MS.gene68810:intron | 10.0% |
| !!! | TTGAATTGGATCTATTTTTG+TGG | + | chr8.4:81670946-81670965 | MS.gene68810:intron | 25.0% |
| ATTCCAGCAATTATTCCCAA+AGG | - | chr8.4:81670804-81670823 | None:intergenic | 35.0% | |
| TTGGAAGCTATGTTACCTTT+GGG | + | chr8.4:81670786-81670805 | MS.gene68810:CDS | 35.0% | |
| ! | TTACCTTTGGGAATAATTGC+TGG | + | chr8.4:81670798-81670817 | MS.gene68810:CDS | 35.0% |
| AAATCATTGCCGATGTGCTT+AGG | - | chr8.4:81670976-81670995 | None:intergenic | 40.0% | |
| GCTGGAATGCTTTGTGTTAT+GGG | + | chr8.4:81670816-81670835 | MS.gene68810:CDS | 40.0% | |
| GTTGGAAGCTATGTTACCTT+TGG | + | chr8.4:81670785-81670804 | MS.gene68810:CDS | 40.0% | |
| TGCTGGAATGCTTTGTGTTA+TGG | + | chr8.4:81670815-81670834 | MS.gene68810:CDS | 40.0% | |
| AGCACATCGGCAATGATTTG+TGG | + | chr8.4:81670977-81670996 | MS.gene68810:CDS | 45.0% | |
| ! | GCACATCGGCAATGATTTGT+GGG | + | chr8.4:81670978-81670997 | MS.gene68810:CDS | 45.0% |
| !! | TGTGGTTAGCCTAAGCACAT+CGG | + | chr8.4:81670964-81670983 | MS.gene68810:intron | 45.0% |
| CTTCTTGTCCCTACGTTCCA+TGG | - | chr8.4:81671010-81671029 | None:intergenic | 50.0% | |
| TACATCCACAGAGCTGCTCA+CGG | + | chr8.4:81670852-81670871 | MS.gene68810:CDS | 50.0% | |
| ! | CGGCAATGATTTGTGGGACG+TGG | + | chr8.4:81670984-81671003 | MS.gene68810:CDS | 55.0% |
| ! | TGATTTGTGGGACGTGGCCA+TGG | + | chr8.4:81670990-81671009 | MS.gene68810:CDS | 55.0% |
| GGACGTGGCCATGGAACGTA+GGG | + | chr8.4:81670999-81671018 | MS.gene68810:CDS | 60.0% | |
| CTCGTCCGTGAGCAGCTCTG+TGG | - | chr8.4:81670860-81670879 | None:intergenic | 65.0% | |
| GGGACGTGGCCATGGAACGT+AGG | + | chr8.4:81670998-81671017 | MS.gene68810:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 81670771 | 81671059 | 81670771 | ID=MS.gene68810 |
| chr8.4 | mRNA | 81670771 | 81671059 | 81670771 | ID=MS.gene68810.t1;Parent=MS.gene68810 |
| chr8.4 | exon | 81670771 | 81670878 | 81670771 | ID=MS.gene68810.t1.exon1;Parent=MS.gene68810.t1 |
| chr8.4 | CDS | 81670771 | 81670878 | 81670771 | ID=cds.MS.gene68810.t1;Parent=MS.gene68810.t1 |
| chr8.4 | exon | 81670973 | 81671059 | 81670973 | ID=MS.gene68810.t1.exon2;Parent=MS.gene68810.t1 |
| chr8.4 | CDS | 81670973 | 81671059 | 81670973 | ID=cds.MS.gene68810.t1;Parent=MS.gene68810.t1 |
| Gene Sequence |
| Protein sequence |
