AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073083


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073083.t1 MTR_2g085025 98.851 174 2 0 1 174 1 174 1.74e-123 345
MS.gene073083.t1 MTR_7g104020 67.742 155 48 1 20 174 14 166 1.10e-61 188
MS.gene073083.t1 MTR_7g104020 66.456 158 48 2 20 174 14 169 1.27e-59 183
MS.gene073083.t1 MTR_1g492930 56.329 158 68 1 17 173 1 158 3.93e-51 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073083.t1 AT2G27740 59.649 171 68 1 1 171 1 170 4.65e-68 205
MS.gene073083.t1 AT2G36410 67.647 136 44 0 38 173 57 192 2.01e-61 189
MS.gene073083.t1 AT3G52920 68.889 135 42 0 38 172 28 162 3.93e-61 187
MS.gene073083.t1 AT3G09980 70.139 144 43 0 31 174 35 178 9.03e-60 184
MS.gene073083.t1 AT2G36410 66.187 139 44 1 38 173 57 195 2.42e-59 183
MS.gene073083.t1 AT3G52920 67.391 138 42 1 38 172 28 165 6.10e-59 182
MS.gene073083.t1 AT5G03660 66.418 134 45 0 38 171 36 169 2.16e-49 157
MS.gene073083.t1 AT3G52900 51.974 152 73 0 23 174 13 164 1.47e-48 155
MS.gene073083.t1 AT2G36355 51.299 154 69 2 22 173 5 154 1.67e-44 144
MS.gene073083.t1 AT2G27740 53.659 123 56 1 1 123 1 122 1.12e-41 136
MS.gene073083.t1 AT5G03660 63.793 116 42 0 38 153 36 151 8.52e-40 132
MS.gene073083.t1 AT2G36410 66.279 86 29 0 38 123 57 142 3.19e-35 120
MS.gene073083.t1 AT5G03660 53.731 134 40 1 38 171 36 147 9.69e-32 112
MS.gene073083.t1 AT2G36355 62.651 83 31 0 91 173 1 83 1.15e-30 107
MS.gene073083.t1 AT2G36355 61.628 86 33 0 88 173 27 112 1.43e-30 107
MS.gene073083.t1 AT2G36355 44.144 111 55 3 22 130 5 110 3.07e-22 87.0

Find 30 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCTAACCTCCACTAATT+TGG 0.202427 2.2:-12584946 None:intergenic
AGAAATTGAGAGAAGAAAAT+TGG 0.229998 2.2:+12583589 MS.gene073083:CDS
ATTGCAACATCCTTCCTCTT+TGG 0.283049 2.2:-12584256 None:intergenic
TTTCATCTCTTCTTGGACTC+TGG 0.297684 2.2:-12583514 None:intergenic
GGGTCAAACCTGCCAGAAAA+AGG 0.317850 2.2:+12584324 MS.gene073083:CDS
GTGCAACATGTGAAGAATTC+AGG 0.351410 2.2:+12583476 MS.gene073083:CDS
TCTAGCAATGTTTAGAGAAA+AGG 0.359449 2.2:+12583562 MS.gene073083:CDS
TTCTGGCAGGTTTGACCCAA+TGG 0.366095 2.2:-12584319 None:intergenic
AAAATTGGAGGTTAGAGATA+AGG 0.419428 2.2:+12583604 MS.gene073083:CDS
GAGTCCAAGAAGAGATGAAA+AGG 0.433573 2.2:+12583517 MS.gene073083:CDS
AACAAAGAGTTGAAGCCATT+GGG 0.443322 2.2:+12584304 MS.gene073083:CDS
AGAAATTGAATAGGAAATTG+AGG 0.443344 2.2:+12584222 MS.gene073083:intron
GAGGATAAGGATGAAGAAGC+TGG 0.468797 2.2:+12585096 MS.gene073083:CDS
CGAAAAGGAAACAAAGAGAT+TGG 0.472358 2.2:+12583649 MS.gene073083:CDS
TCAACTTGTTACCAAATTAG+TGG 0.474598 2.2:+12584935 MS.gene073083:CDS
GAAATTGAATAGGAAATTGA+GGG 0.474638 2.2:+12584223 MS.gene073083:intron
TAACAAAGAGTTGAAGCCAT+TGG 0.493465 2.2:+12584303 MS.gene073083:CDS
GATAAGGTTCATGCTCATCT+TGG 0.506717 2.2:+12583620 MS.gene073083:CDS
TCATCTTGGTCGAGTCGAAA+AGG 0.517837 2.2:+12583634 MS.gene073083:CDS
TGACTGAAAGTGAGAGGATA+AGG 0.518680 2.2:+12585083 MS.gene073083:CDS
AAGAAGAGATGAAAAGGAAG+AGG 0.527146 2.2:+12583523 MS.gene073083:CDS
AATTGAGAGAAGAAAATTGG+AGG 0.531288 2.2:+12583592 MS.gene073083:CDS
AGAATACAATGAAGCTCTTG+AGG 0.569694 2.2:+12584884 MS.gene073083:CDS
GTTCACAGATCCAAAGAGGA+AGG 0.618763 2.2:+12584246 MS.gene073083:CDS
AGCTTGTGACTGAAAGTGAG+AGG 0.623994 2.2:+12585077 MS.gene073083:intron
ACTTGTTACCAAATTAGTGG+AGG 0.627774 2.2:+12584938 MS.gene073083:CDS
AGGGGTTCACAGATCCAAAG+AGG 0.671132 2.2:+12584242 MS.gene073083:CDS
AAATTGAATAGGAAATTGAG+GGG 0.691169 2.2:+12584224 MS.gene073083:intron
GATAAGGATGAAGAAGCTGG+AGG 0.694341 2.2:+12585099 MS.gene073083:CDS
AGTTAGAAAGAAGATAGACA+TGG 0.715757 2.2:+12584279 MS.gene073083:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATAAAATCAAATG+TGG + chr2.2:12584125-12584144 MS.gene073083:intron 10.0%
!!! TATTTTTTAAAAACAATGTA+CGG - chr2.2:12584779-12584798 None:intergenic 10.0%
!! ATATGTTTATTTAGTTGTTT+AGG + chr2.2:12584565-12584584 MS.gene073083:intron 15.0%
!! CAAACAAAGAATATTTATAA+TGG - chr2.2:12584202-12584221 None:intergenic 15.0%
!! GAAATTAAAGCAAATAATTA+AGG - chr2.2:12584501-12584520 None:intergenic 15.0%
!! TTATAATATCTTTACAACAT+AGG + chr2.2:12584394-12584413 MS.gene073083:intron 15.0%
!!! AATTATTTGCTTTAATTTCT+TGG + chr2.2:12584502-12584521 MS.gene073083:intron 15.0%
!!! TTGTGAAACTATATATTTTT+GGG + chr2.2:12584849-12584868 MS.gene073083:intron 15.0%
!! AAAAAAGAAATATGTTAGCT+AGG - chr2.2:12585022-12585041 None:intergenic 20.0%
!! ATTAAAGCAAATAATTAAGG+TGG - chr2.2:12584498-12584517 None:intergenic 20.0%
!! TTTGATATTGTAGAAAATCA+CGG + chr2.2:12583887-12583906 MS.gene073083:intron 20.0%
!!! CTTGTGAAACTATATATTTT+TGG + chr2.2:12584848-12584867 MS.gene073083:intron 20.0%
!!! CTTTTGATGACATAATATAT+GGG - chr2.2:12584529-12584548 None:intergenic 20.0%
!!! TGTGAAACTATATATTTTTG+GGG + chr2.2:12584850-12584869 MS.gene073083:intron 20.0%
! AAATTGAATAGGAAATTGAG+GGG + chr2.2:12584224-12584243 MS.gene073083:intron 25.0%
! AAGTTTCAAAATAATGGTCA+CGG + chr2.2:12583840-12583859 MS.gene073083:intron 25.0%
! AGAAATTGAATAGGAAATTG+AGG + chr2.2:12584222-12584241 MS.gene073083:intron 25.0%
! AGAAATTGAGAGAAGAAAAT+TGG + chr2.2:12583589-12583608 MS.gene073083:CDS 25.0%
! ATATCAAAAGCAAAACACAT+TGG - chr2.2:12584445-12584464 None:intergenic 25.0%
! ATGATGTTTCATTGTTTGTT+TGG + chr2.2:12583728-12583747 MS.gene073083:intron 25.0%
! CAAATTCAGTGTTTGTTTAT+TGG - chr2.2:12583695-12583714 None:intergenic 25.0%
! CAATGAGAAAAACAAAGAAA+AGG + chr2.2:12584911-12584930 MS.gene073083:CDS 25.0%
! CCATATATTATGTCATCAAA+AGG + chr2.2:12584527-12584546 MS.gene073083:intron 25.0%
! GAAATTGAATAGGAAATTGA+GGG + chr2.2:12584223-12584242 MS.gene073083:intron 25.0%
! TATATTATGTCATCAAAAGG+AGG + chr2.2:12584530-12584549 MS.gene073083:intron 25.0%
! TATCAAAAGCAAAACACATT+GGG - chr2.2:12584444-12584463 None:intergenic 25.0%
! TGTGCAAAGTTTCAAAATAA+TGG + chr2.2:12583834-12583853 MS.gene073083:intron 25.0%
! TTACATTTGATCTATAGTCA+AGG + chr2.2:12584041-12584060 MS.gene073083:intron 25.0%
!! CTTTGTTTGAGAAATTGAAT+AGG + chr2.2:12584213-12584232 MS.gene073083:intron 25.0%
!!! ATTGTTTGTTTGGTTTTTAC+TGG + chr2.2:12583738-12583757 MS.gene073083:intron 25.0%
!!! CCTTTTGATGACATAATATA+TGG - chr2.2:12584530-12584549 None:intergenic 25.0%
!!! TTCACTTGTTTGGTTTTTAT+TGG + chr2.2:12583791-12583810 MS.gene073083:intron 25.0%
AAAATTGGAGGTTAGAGATA+AGG + chr2.2:12583604-12583623 MS.gene073083:CDS 30.0%
AACAGTTAGTACTAAACAGT+TGG - chr2.2:12584359-12584378 None:intergenic 30.0%
AATTGAGAGAAGAAAATTGG+AGG + chr2.2:12583592-12583611 MS.gene073083:CDS 30.0%
AATTTGAATTCCGTCTGTTT+CGG - chr2.2:12584702-12584721 None:intergenic 30.0%
ACAATATAACGTTGAAATCG+TGG + chr2.2:12583960-12583979 MS.gene073083:intron 30.0%
AGTTAGAAAGAAGATAGACA+TGG + chr2.2:12584279-12584298 MS.gene073083:CDS 30.0%
TAAAGCTAATCCTTAAATCC+TGG - chr2.2:12584024-12584043 None:intergenic 30.0%
TCTAGCAATGTTTAGAGAAA+AGG + chr2.2:12583562-12583581 MS.gene073083:CDS 30.0%
TTAAAAACAATGTACGGAAG+AGG - chr2.2:12584773-12584792 None:intergenic 30.0%
! CTTTGCATTATGTGAACTTT+TGG + chr2.2:12585051-12585070 MS.gene073083:intron 30.0%
! TACAACATAGGATTTGTTCA+AGG + chr2.2:12584406-12584425 MS.gene073083:intron 30.0%
! TGCTGATCATATGATCTTTT+TGG - chr2.2:12583440-12583459 None:intergenic 30.0%
! TGGAAAATCTAACCTTTTTC+TGG - chr2.2:12584339-12584358 None:intergenic 30.0%
!! TCAACTTGTTACCAAATTAG+TGG + chr2.2:12584935-12584954 MS.gene073083:CDS 30.0%
!!! CTATATATTTTTGGGGTTAC+AGG + chr2.2:12584857-12584876 MS.gene073083:intron 30.0%
!!! CTGGTCAAGGTTTTAAAAAA+CGG - chr2.2:12584005-12584024 None:intergenic 30.0%
AAAACCTTGACCAGGATTTA+AGG + chr2.2:12584011-12584030 MS.gene073083:intron 35.0%
AACAAAGAGTTGAAGCCATT+GGG + chr2.2:12584304-12584323 MS.gene073083:CDS 35.0%
AAGAAGAGATGAAAAGGAAG+AGG + chr2.2:12583523-12583542 MS.gene073083:CDS 35.0%
ACTTCTAACCTCCACTAATT+TGG - chr2.2:12584949-12584968 None:intergenic 35.0%
AGAATACAATGAAGCTCTTG+AGG + chr2.2:12584884-12584903 MS.gene073083:CDS 35.0%
AGACTAAAGTCTAAGCAATG+TGG - chr2.2:12584470-12584489 None:intergenic 35.0%
CAATATCAAAGATTACTGCG+TGG - chr2.2:12583877-12583896 None:intergenic 35.0%
CGAAAAGGAAACAAAGAGAT+TGG + chr2.2:12583649-12583668 MS.gene073083:CDS 35.0%
CGGAATTCAAATTTCTCAGT+TGG + chr2.2:12584709-12584728 MS.gene073083:intron 35.0%
TAACAAAGAGTTGAAGCCAT+TGG + chr2.2:12584303-12584322 MS.gene073083:CDS 35.0%
TAATCCTTAAATCCTGGTCA+AGG - chr2.2:12584018-12584037 None:intergenic 35.0%
TAGCGACCACAATTTGATAT+CGG + chr2.2:12583935-12583954 MS.gene073083:intron 35.0%
TGTGCAAAGTTTCACTTGTT+TGG + chr2.2:12583781-12583800 MS.gene073083:intron 35.0%
TTGTCACCGATATCAAATTG+TGG - chr2.2:12583944-12583963 None:intergenic 35.0%
! ACTTGTTACCAAATTAGTGG+AGG + chr2.2:12584938-12584957 MS.gene073083:CDS 35.0%
! TCTTCCTTTTCATCTCTTCT+TGG - chr2.2:12583524-12583543 None:intergenic 35.0%
!! AAATCTAACCTTTTTCTGGC+AGG - chr2.2:12584335-12584354 None:intergenic 35.0%
!! CTAACTAGCTTGTTTCATAC+AGG - chr2.2:12584094-12584113 None:intergenic 35.0%
!!! CGTTTTTTAAAACCTTGACC+AGG + chr2.2:12584003-12584022 MS.gene073083:intron 35.0%
ATTGCAACATCCTTCCTCTT+TGG - chr2.2:12584259-12584278 None:intergenic 40.0%
GACTTTGTAACCGAAACAGA+CGG + chr2.2:12584689-12584708 MS.gene073083:intron 40.0%
GAGTCCAAGAAGAGATGAAA+AGG + chr2.2:12583517-12583536 MS.gene073083:CDS 40.0%
GATAAGGTTCATGCTCATCT+TGG + chr2.2:12583620-12583639 MS.gene073083:CDS 40.0%
GTCAATCTCTTACCACCAAA+CGG - chr2.2:12584672-12584691 None:intergenic 40.0%
GTGCAACATGTGAAGAATTC+AGG + chr2.2:12583476-12583495 MS.gene073083:CDS 40.0%
TCAAATTGTGGTCGCTATTG+CGG - chr2.2:12583932-12583951 None:intergenic 40.0%
TGACTGAAAGTGAGAGGATA+AGG + chr2.2:12585083-12585102 MS.gene073083:CDS 40.0%
TTTCATCTCTTCTTGGACTC+TGG - chr2.2:12583517-12583536 None:intergenic 40.0%
AGCTTGTGACTGAAAGTGAG+AGG + chr2.2:12585077-12585096 MS.gene073083:intron 45.0%
ATCTCTTACCACCAAACGGA+AGG - chr2.2:12584668-12584687 None:intergenic 45.0%
GAGGATAAGGATGAAGAAGC+TGG + chr2.2:12585096-12585115 MS.gene073083:CDS 45.0%
GATAAGGATGAAGAAGCTGG+AGG + chr2.2:12585099-12585118 MS.gene073083:CDS 45.0%
GTTCACAGATCCAAAGAGGA+AGG + chr2.2:12584246-12584265 MS.gene073083:CDS 45.0%
TCATCTTGGTCGAGTCGAAA+AGG + chr2.2:12583634-12583653 MS.gene073083:CDS 45.0%
TGCTTCTTGTTCCTTCCGTT+TGG + chr2.2:12584654-12584673 MS.gene073083:intron 45.0%
TTCTTGTTCCTTCCGTTTGG+TGG + chr2.2:12584657-12584676 MS.gene073083:intron 45.0%
! TAATGGTCACGGTCACTTTG+CGG + chr2.2:12583851-12583870 MS.gene073083:intron 45.0%
AGGGGTTCACAGATCCAAAG+AGG + chr2.2:12584242-12584261 MS.gene073083:CDS 50.0%
GGGTCAAACCTGCCAGAAAA+AGG + chr2.2:12584324-12584343 MS.gene073083:CDS 50.0%
TTCTGGCAGGTTTGACCCAA+TGG - chr2.2:12584322-12584341 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 12583428 12585153 12583428 ID=MS.gene073083
chr2.2 mRNA 12583428 12585153 12583428 ID=MS.gene073083.t1;Parent=MS.gene073083
chr2.2 exon 12583428 12583685 12583428 ID=MS.gene073083.t1.exon1;Parent=MS.gene073083.t1
chr2.2 CDS 12583428 12583685 12583428 ID=cds.MS.gene073083.t1;Parent=MS.gene073083.t1
chr2.2 exon 12584235 12584345 12584235 ID=MS.gene073083.t1.exon2;Parent=MS.gene073083.t1
chr2.2 CDS 12584235 12584345 12584235 ID=cds.MS.gene073083.t1;Parent=MS.gene073083.t1
chr2.2 exon 12584879 12584959 12584879 ID=MS.gene073083.t1.exon3;Parent=MS.gene073083.t1
chr2.2 CDS 12584879 12584959 12584879 ID=cds.MS.gene073083.t1;Parent=MS.gene073083.t1
chr2.2 exon 12585079 12585153 12585079 ID=MS.gene073083.t1.exon4;Parent=MS.gene073083.t1
chr2.2 CDS 12585079 12585153 12585079 ID=cds.MS.gene073083.t1;Parent=MS.gene073083.t1
Gene Sequence

>MS.gene073083

ATGGCAACACCAAAAAGATCATATGATCAGCAACAACAACTACAGATTGTGCAACATGTGAAGAATTCAGGAATGATTAACAACATCCAGAGTCCAAGAAGAGATGAAAAGGAAGAGGAAATGTCAAGATCAGTTCTAGCAATGTTTAGAGAAAAGGAAGAAGAAATTGAGAGAAGAAAATTGGAGGTTAGAGATAAGGTTCATGCTCATCTTGGTCGAGTCGAAAAGGAAACAAAGAGATTGGCTGAGATAAGAGAAGTAAGTCCAATAAACAAACACTGAATTTGAGCTAGATTGTTTATGATGTTTCATTGTTTGTTTGGTTTTTACTGGTTTATGCATTTATAAATCATTGTGCAAAGTTTCACTTGTTTGGTTTTTATTGGTTTATGTATTTATAAATCTTTGTGCAAAGTTTCAAAATAATGGTCACGGTCACTTTGCGGCCACGCAGTAATCTTTGATATTGTAGAAAATCACGGATAAATACAACTTGTGTTGCCGCAATAGCGACCACAATTTGATATCGGTGACAATATAACGTTGAAATCGTGGCTATATTTGCTGTTGCAGACCGTTTTTTAAAACCTTGACCAGGATTTAAGGATTAGCTTTACATTTGATCTATAGTCAAGGAAAATTGTATTCACATGTTCATATATACCTGTATGAAACAAGCTAGTTAGAAACAATTTAAAAATAAAATAAAATCAAATGTGGTATTTTTTTTTTAAAATTATTCACATTTTGTTATATTCAAGATTTTTTGATCCATTATAAATATTCTTTGTTTGAGAAATTGAATAGGAAATTGAGGGGTTCACAGATCCAAAGAGGAAGGATGTTGCAATAGTTAGAAAGAAGATAGACATGGTTAACAAAGAGTTGAAGCCATTGGGTCAAACCTGCCAGAAAAAGGTTAGATTTTCCAACTGTTTAGTACTAACTGTTGTTTGCTTTATTCTTTTATAATATCTTTACAACATAGGATTTGTTCAAGGAGACAAAGTGATCCCAATGTGTTTTGCTTTTGATATAACCACATTGCTTAGACTTTAGTCTATTGACCACCTTAATTATTTGCTTTAATTTCTTGGTCCCATATATTATGTCATCAAAAGGAGGCTATATAATACTATATGTTTATTTAGTTGTTTAGGTGATTATTCAAAATAATTGTGTTTGAATTTGCAAGTAGAAAATTGCTATTTGAAATTCGTCATACATGCTTCTTGTTCCTTCCGTTTGGTGGTAAGAGATTGACTTTGTAACCGAAACAGACGGAATTCAAATTTCTCAGTTGGAAAATGTCATTTTTGCAACTTTAATTTATGATAATTTTCCTCTTCCGTACATTGTTTTTAAAAAATAAATTAAAACAAGAATGAAAGTATGTTAACTATAAAAGTTTCTGAATCTTCTTGTGAAACTATATATTTTTGGGGTTACAGGAGAGAGAATACAATGAAGCTCTTGAGGCTTTCAATGAGAAAAACAAAGAAAAGGCTCAACTTGTTACCAAATTAGTGGAGGTTAGAAGTTATTCATTGATTTAAATGTTAGTACACTAACAATATCATATATTTTTGTTCCTAGCTAACATATTTCTTTTTTATTCTCTCTCTTTGCATTATGTGAACTTTTGGATTAGCTTGTGACTGAAAGTGAGAGGATAAGGATGAAGAAGCTGGAGGATCTAAGCAAAAATATAGATACACTTCACTAA

Protein sequence

>MS.gene073083.t1

MATPKRSYDQQQQLQIVQHVKNSGMINNIQSPRRDEKEEEMSRSVLAMFREKEEEIERRKLEVRDKVHAHLGRVEKETKRLAEIREEIEGFTDPKRKDVAIVRKKIDMVNKELKPLGQTCQKKEREYNEALEAFNEKNKEKAQLVTKLVELVTESERIRMKKLEDLSKNIDTLH