AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62806


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62806.t1 MTR_4g102740 96.786 280 9 0 1 280 1 280 0.0 543
MS.gene62806.t1 MTR_0086s0010 45.724 304 118 4 20 280 22 321 6.55e-79 243
MS.gene62806.t1 MTR_1g101440 47.902 286 114 5 28 280 18 301 5.06e-76 235
MS.gene62806.t1 MTR_2g018860 39.759 249 114 5 32 279 30 243 2.42e-53 174
MS.gene62806.t1 MTR_7g069610 44.554 202 111 1 32 232 19 220 1.68e-47 158
MS.gene62806.t1 MTR_4g017810 37.179 234 119 2 47 280 19 224 2.80e-40 140
MS.gene62806.t1 MTR_2g018860 39.109 202 88 4 79 279 34 201 2.15e-35 126
MS.gene62806.t1 MTR_6g022040 41.176 119 61 2 24 136 22 137 3.57e-21 87.8
MS.gene62806.t1 MTR_6g022020 43.810 105 57 1 32 136 38 140 3.81e-21 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62806.t1 AT5G52060 46.545 275 119 3 34 280 41 315 1.95e-72 226
MS.gene62806.t1 AT5G62100 45.238 252 131 3 31 280 14 260 1.53e-66 209
MS.gene62806.t1 AT5G07220 44.615 260 132 4 32 280 21 279 3.89e-65 206
MS.gene62806.t1 AT5G62100 43.726 263 130 5 31 280 14 271 2.45e-63 201
MS.gene62806.t1 AT5G62100 51.087 184 89 1 31 214 14 196 1.40e-56 181
MS.gene62806.t1 AT5G62100 50.543 184 90 1 31 214 14 196 5.47e-56 179
MS.gene62806.t1 AT3G51780 35.985 264 124 5 31 279 9 242 8.95e-46 155
MS.gene62806.t1 AT5G62100 54.412 136 61 1 31 166 14 148 4.53e-44 147
MS.gene62806.t1 AT5G62100 54.412 136 61 1 31 166 14 148 4.53e-44 147
MS.gene62806.t1 AT5G14360 40.000 105 60 1 33 137 49 150 2.03e-20 86.3
MS.gene62806.t1 AT5G40630 39.815 108 62 2 26 132 50 155 1.24e-19 84.0

Find 41 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATTATTGTGTACCTTTC+TGG 0.064078 8.1:-21036960 None:intergenic
ATTACATTGGATGAAATTTA+TGG 0.207184 8.1:+21037165 MS.gene62806:CDS
CTCTAAGGTTACTTCAATTA+AGG 0.213411 8.1:-21036924 None:intergenic
CTTAATTGAAGTAACCTTAG+AGG 0.272826 8.1:+21036925 MS.gene62806:CDS
CATGAGAACTGATATGAATT+TGG 0.287339 8.1:-21036570 None:intergenic
ATCAGTTCTCATGCAAGTTT+TGG 0.294978 8.1:+21036580 MS.gene62806:CDS
GGCAAGTTTGTCAACCTCTA+AGG 0.304140 8.1:-21036939 None:intergenic
GAGTAGAGAAAGAAGGATAT+TGG 0.354055 8.1:+21036862 MS.gene62806:CDS
ATCAAAGTCAAAGTGAAGTA+TGG 0.369232 8.1:+21036535 MS.gene62806:CDS
CTTCACTTTGACTTTGATGA+TGG 0.373301 8.1:-21036530 None:intergenic
AATTCCCATTCAGCTACATT+TGG 0.373777 8.1:-21036448 None:intergenic
TTAATCAAAAGTTTCCCATT+TGG 0.385906 8.1:-21037498 None:intergenic
CAATCCAAATGTAGCTGAAT+GGG 0.407553 8.1:+21036444 MS.gene62806:CDS
AATTTGGTAGGTTTCAGCTT+TGG 0.444763 8.1:+21037065 MS.gene62806:intron
TAACATAACAAAGGAGAGTA+AGG 0.457931 8.1:+21037388 MS.gene62806:CDS
TCAATCCAAATGTAGCTGAA+TGG 0.461891 8.1:+21036443 MS.gene62806:CDS
TTGGAGGGTAATGTTTCTAA+AGG 0.464546 8.1:+21037084 MS.gene62806:CDS
AAGAATTGCAAAGAAGGAAA+AGG 0.466398 8.1:+21036892 MS.gene62806:CDS
GAAATTGCAGAGAAAAGAAC+AGG 0.476993 8.1:+21037200 MS.gene62806:CDS
CAGTTGTAGTAACAACCAAA+TGG 0.480586 8.1:+21037483 MS.gene62806:CDS
TTGGTAGGTTTCAGCTTTGG+AGG 0.481735 8.1:+21037068 MS.gene62806:intron
GAAATTAAGAATTGCAAAGA+AGG 0.485109 8.1:+21036886 MS.gene62806:CDS
AGGTGAAGTTATTTCAGAGT+TGG 0.494651 8.1:+21037104 MS.gene62806:CDS
AGTTGTAGTAACAACCAAAT+GGG 0.521225 8.1:+21037484 MS.gene62806:CDS
GGTTGACAAACTTGCCAGAA+AGG 0.524609 8.1:+21036946 MS.gene62806:CDS
AACTGATATGAATTTGGTGA+TGG 0.530668 8.1:-21036564 None:intergenic
AAAATGTTGACAGAATCAAC+AGG 0.539679 8.1:+21036722 MS.gene62806:CDS
GATATTGAGAGAGTCAAAGA+TGG 0.547900 8.1:+21036809 MS.gene62806:CDS
ACATCGAGAGTAGAGAAAGA+AGG 0.558063 8.1:+21036855 MS.gene62806:CDS
ACTGACTCAGAATGTTTCAA+AGG 0.560272 8.1:-21037464 None:intergenic
GATGAGAGTATTGATTACAT+TGG 0.562495 8.1:+21037152 MS.gene62806:CDS
TTGGATGAAATTTATGGTGA+GGG 0.566235 8.1:+21037171 MS.gene62806:CDS
AAAACTTGCAATGCAGTCAA+AGG 0.573016 8.1:+21036357 None:intergenic
TGGTAGGTTTCAGCTTTGGA+GGG 0.580586 8.1:+21037069 MS.gene62806:intron
ATTTGGATTGACATGAACAT+TGG 0.587267 8.1:-21036431 None:intergenic
GCTGAATGGGAATTAAGACC+AGG 0.631294 8.1:+21036457 MS.gene62806:CDS
ACTGATATGAATTTGGTGAT+GGG 0.633798 8.1:-21036563 None:intergenic
ATTGGATGAAATTTATGGTG+AGG 0.644553 8.1:+21037170 MS.gene62806:CDS
TGCAGTCAAAGGTTACAAAG+AGG 0.668299 8.1:+21036368 MS.gene62806:CDS
GAATGGGAATTAAGACCAGG+TGG 0.675489 8.1:+21036460 MS.gene62806:CDS
AAATAACAATAACATAACAA+AGG 0.682250 8.1:+21037379 MS.gene62806:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAATTAATGTGT+AGG + chr8.1:21037295-21037314 MS.gene62806:intron 10.0%
!! AAATAACAATAACATAACAA+AGG + chr8.1:21037379-21037398 MS.gene62806:CDS 15.0%
!! CTAAATAATTCAATGAAATT+TGG + chr8.1:21037049-21037068 MS.gene62806:intron 15.0%
!! TGATTATTATTGGATTATTA+TGG + chr8.1:21036674-21036693 MS.gene62806:intron 15.0%
!! TTAAAATATGCAAAGTATAA+AGG - chr8.1:21036630-21036649 None:intergenic 15.0%
!! AAGTATAAAGGAAATTCTAA+AGG - chr8.1:21036618-21036637 None:intergenic 20.0%
!! ATAAAATTAATGTGTAGGTT+AGG + chr8.1:21037300-21037319 MS.gene62806:intron 20.0%
!! ATAATTCAATGAAATTTGGT+AGG + chr8.1:21037053-21037072 MS.gene62806:intron 20.0%
!! ATTACATTGGATGAAATTTA+TGG + chr8.1:21037165-21037184 MS.gene62806:CDS 20.0%
!! TTTCAACATTGTAAAAATAG+TGG + chr8.1:21037026-21037045 MS.gene62806:intron 20.0%
!!! TGATTGATGATGATTATTAT+TGG + chr8.1:21036664-21036683 MS.gene62806:intron 20.0%
! GAAATTAAGAATTGCAAAGA+AGG + chr8.1:21036886-21036905 MS.gene62806:CDS 25.0%
! TTAATCAAAAGTTTCCCATT+TGG - chr8.1:21037501-21037520 None:intergenic 25.0%
!! TTGGATTATTATGGTTTTTG+TGG + chr8.1:21036683-21036702 MS.gene62806:intron 25.0%
AAAATGTTGACAGAATCAAC+AGG + chr8.1:21036722-21036741 MS.gene62806:CDS 30.0%
AAGAATTGCAAAGAAGGAAA+AGG + chr8.1:21036892-21036911 MS.gene62806:CDS 30.0%
AGTTGTAGTAACAACCAAAT+GGG + chr8.1:21037484-21037503 MS.gene62806:CDS 30.0%
ATCAAAGTCAAAGTGAAGTA+TGG + chr8.1:21036535-21036554 MS.gene62806:CDS 30.0%
ATTGGATGAAATTTATGGTG+AGG + chr8.1:21037170-21037189 MS.gene62806:CDS 30.0%
ATTTGGATTGACATGAACAT+TGG - chr8.1:21036434-21036453 None:intergenic 30.0%
CAGTTATGAAAACAAAGAGT+TGG - chr8.1:21037229-21037248 None:intergenic 30.0%
CATGAGAACTGATATGAATT+TGG - chr8.1:21036573-21036592 None:intergenic 30.0%
CTTAATTGAAGTAACCTTAG+AGG + chr8.1:21036925-21036944 MS.gene62806:CDS 30.0%
TAACATAACAAAGGAGAGTA+AGG + chr8.1:21037388-21037407 MS.gene62806:CDS 30.0%
TTGGATGAAATTTATGGTGA+GGG + chr8.1:21037171-21037190 MS.gene62806:CDS 30.0%
! AACGATCAACATGTTTTCAA+GGG + chr8.1:21036393-21036412 MS.gene62806:CDS 30.0%
! AACTGATATGAATTTGGTGA+TGG - chr8.1:21036567-21036586 None:intergenic 30.0%
! ACTGATATGAATTTGGTGAT+GGG - chr8.1:21036566-21036585 None:intergenic 30.0%
! ATTATGGTTTTTGTGGTGTA+GGG + chr8.1:21036690-21036709 MS.gene62806:intron 30.0%
! TATTATGGTTTTTGTGGTGT+AGG + chr8.1:21036689-21036708 MS.gene62806:intron 30.0%
!! CTCTAAGGTTACTTCAATTA+AGG - chr8.1:21036927-21036946 None:intergenic 30.0%
!! GATGAGAGTATTGATTACAT+TGG + chr8.1:21037152-21037171 MS.gene62806:CDS 30.0%
!!! CAACTCTTTGTTTTCATAAC+TGG + chr8.1:21037227-21037246 MS.gene62806:intron 30.0%
AATTCCCATTCAGCTACATT+TGG - chr8.1:21036451-21036470 None:intergenic 35.0%
AATTTGGTAGGTTTCAGCTT+TGG + chr8.1:21037065-21037084 MS.gene62806:intron 35.0%
CAATCCAAATGTAGCTGAAT+GGG + chr8.1:21036444-21036463 MS.gene62806:CDS 35.0%
CACATTATTGTGTACCTTTC+TGG - chr8.1:21036963-21036982 None:intergenic 35.0%
CAGTTGTAGTAACAACCAAA+TGG + chr8.1:21037483-21037502 MS.gene62806:CDS 35.0%
GAAATTGCAGAGAAAAGAAC+AGG + chr8.1:21037200-21037219 MS.gene62806:CDS 35.0%
GAGTAGAGAAAGAAGGATAT+TGG + chr8.1:21036862-21036881 MS.gene62806:CDS 35.0%
GATATTGAGAGAGTCAAAGA+TGG + chr8.1:21036809-21036828 MS.gene62806:CDS 35.0%
TCAATCCAAATGTAGCTGAA+TGG + chr8.1:21036443-21036462 MS.gene62806:CDS 35.0%
! ACTGACTCAGAATGTTTCAA+AGG - chr8.1:21037467-21037486 None:intergenic 35.0%
! AGGTGAAGTTATTTCAGAGT+TGG + chr8.1:21037104-21037123 MS.gene62806:CDS 35.0%
! ATCAGTTCTCATGCAAGTTT+TGG + chr8.1:21036580-21036599 MS.gene62806:CDS 35.0%
! CAACGATCAACATGTTTTCA+AGG + chr8.1:21036392-21036411 MS.gene62806:CDS 35.0%
! CTTCACTTTGACTTTGATGA+TGG - chr8.1:21036533-21036552 None:intergenic 35.0%
! TTATGGTTTTTGTGGTGTAG+GGG + chr8.1:21036691-21036710 MS.gene62806:intron 35.0%
! TTGGAGGGTAATGTTTCTAA+AGG + chr8.1:21037084-21037103 MS.gene62806:CDS 35.0%
ACATCGAGAGTAGAGAAAGA+AGG + chr8.1:21036855-21036874 MS.gene62806:CDS 40.0%
TGCAGTCAAAGGTTACAAAG+AGG + chr8.1:21036368-21036387 MS.gene62806:CDS 40.0%
! TTTTGTACTAGCATTCCACC+TGG - chr8.1:21036478-21036497 None:intergenic 40.0%
GAATGGGAATTAAGACCAGG+TGG + chr8.1:21036460-21036479 MS.gene62806:CDS 45.0%
GCTGAATGGGAATTAAGACC+AGG + chr8.1:21036457-21036476 MS.gene62806:CDS 45.0%
GGCAAGTTTGTCAACCTCTA+AGG - chr8.1:21036942-21036961 None:intergenic 45.0%
GGTTGACAAACTTGCCAGAA+AGG + chr8.1:21036946-21036965 MS.gene62806:CDS 45.0%
! TTGGTAGGTTTCAGCTTTGG+AGG + chr8.1:21037068-21037087 MS.gene62806:intron 45.0%
!! TGGTAGGTTTCAGCTTTGGA+GGG + chr8.1:21037069-21037088 MS.gene62806:intron 45.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 21036367 21037520 21036367 ID=MS.gene62806
chr8.1 mRNA 21036367 21037520 21036367 ID=MS.gene62806.t1;Parent=MS.gene62806
chr8.1 exon 21036367 21036601 21036367 ID=MS.gene62806.t1.exon1;Parent=MS.gene62806.t1
chr8.1 CDS 21036367 21036601 21036367 ID=cds.MS.gene62806.t1;Parent=MS.gene62806.t1
chr8.1 exon 21036711 21036967 21036711 ID=MS.gene62806.t1.exon2;Parent=MS.gene62806.t1
chr8.1 CDS 21036711 21036967 21036711 ID=cds.MS.gene62806.t1;Parent=MS.gene62806.t1
chr8.1 exon 21037075 21037221 21037075 ID=MS.gene62806.t1.exon3;Parent=MS.gene62806.t1
chr8.1 CDS 21037075 21037221 21037075 ID=cds.MS.gene62806.t1;Parent=MS.gene62806.t1
chr8.1 exon 21037317 21037520 21037317 ID=MS.gene62806.t1.exon4;Parent=MS.gene62806.t1
chr8.1 CDS 21037317 21037520 21037317 ID=cds.MS.gene62806.t1;Parent=MS.gene62806.t1
Gene Sequence

>MS.gene62806

ATGCAGTCAAAGGTTACAAAGAGGTCAACGATCAACATGTTTTCAAGGGACAAAAAAAGTGACACCAATGTTCATGTCAATCCAAATGTAGCTGAATGGGAATTAAGACCAGGTGGAATGCTAGTACAAAAACGTAATTCAGATGTGAATGATAATTTTTCATCCATCATCAAAGTCAAAGTGAAGTATGGCTCATCCCATCACCAAATTCATATCAGTTCTCATGCAAGTTTTGGTACATACATTCACCTTTAGAATTTCCTTTATACTTTGCATATTTTAATTGATTATATAAATTGATTGATGATGATTATTATTGGATTATTATGGTTTTTGTGGTGTAGGGGAATTGAAGAAAATGTTGACAGAATCAACAGGATTGCATGTTCAAGATCAGAAGTTGATTTTCAAGAAAAAAGAGAGAGACTCAAAGTCATATCTTGATATTGAGAGAGTCAAAGATGGATCAAAGTTGATTTTAATTGAAGACATCGAGAGTAGAGAAAGAAGGATATTGGAGAAATTAAGAATTGCAAAGAAGGAAAAGGCTTCAAAATCCTTAATTGAAGTAACCTTAGAGGTTGACAAACTTGCCAGAAAGGTACACAATAATGTGCATTATTTGTTCAATTGTTACACTCTTTTATTTATTTATTTAATTTCAACATTGTAAAAATAGTGGCTAAATAATTCAATGAAATTTGGTAGGTTTCAGCTTTGGAGGGTAATGTTTCTAAAGGTGAAGTTATTTCAGAGTTGGATGTAGAGAGTTTAACAGAAAATTTGATGAGAGTATTGATTACATTGGATGAAATTTATGGTGAGGGTGAACTGAAATTGCAGAGAAAAGAACAGGTAACCAACTCTTTGTTTTCATAACTGGCTATCTAGATTAAATTAATTGATATTTATAAGTTATGAAAATAAAATAAAATAAAATTAATGTGTAGGTTAGGAGAGTTCAGAAGCACATTGAGACTCTTGATATGTTAAAGATGTCAAAGTCAATTGAAAATAACAATAACATAACAAAGGAGAGTAAGGTTGATTGTGATGTTCAAAAGCAGCATTTGCAAGAGAAGCAACAAGAACAACAACCTTTGAAACATTCTGAGTCAGTTGTAGTAACAACCAAATGGGAAACTTTTGATTAA

Protein sequence

>MS.gene62806.t1

MQSKVTKRSTINMFSRDKKSDTNVHVNPNVAEWELRPGGMLVQKRNSDVNDNFSSIIKVKVKYGSSHHQIHISSHASFGELKKMLTESTGLHVQDQKLIFKKKERDSKSYLDIERVKDGSKLILIEDIESRERRILEKLRIAKKEKASKSLIEVTLEVDKLARKVSALEGNVSKGEVISELDVESLTENLMRVLITLDEIYGEGELKLQRKEQVRRVQKHIETLDMLKMSKSIENNNNITKESKVDCDVQKQHLQEKQQEQQPLKHSESVVVTTKWETFD