AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43075


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43075.t1 MTR_2g085025 98.851 174 2 0 1 174 1 174 1.74e-123 345
MS.gene43075.t1 MTR_7g104020 67.742 155 48 1 20 174 14 166 1.10e-61 188
MS.gene43075.t1 MTR_7g104020 66.456 158 48 2 20 174 14 169 1.27e-59 183
MS.gene43075.t1 MTR_1g492930 56.329 158 68 1 17 173 1 158 3.93e-51 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43075.t1 AT2G27740 59.649 171 68 1 1 171 1 170 4.65e-68 205
MS.gene43075.t1 AT2G36410 67.647 136 44 0 38 173 57 192 2.01e-61 189
MS.gene43075.t1 AT3G52920 68.889 135 42 0 38 172 28 162 3.93e-61 187
MS.gene43075.t1 AT3G09980 70.139 144 43 0 31 174 35 178 9.03e-60 184
MS.gene43075.t1 AT2G36410 66.187 139 44 1 38 173 57 195 2.42e-59 183
MS.gene43075.t1 AT3G52920 67.391 138 42 1 38 172 28 165 6.10e-59 182
MS.gene43075.t1 AT5G03660 66.418 134 45 0 38 171 36 169 2.16e-49 157
MS.gene43075.t1 AT3G52900 51.974 152 73 0 23 174 13 164 1.47e-48 155
MS.gene43075.t1 AT2G36355 51.299 154 69 2 22 173 5 154 1.67e-44 144
MS.gene43075.t1 AT2G27740 53.659 123 56 1 1 123 1 122 1.12e-41 136
MS.gene43075.t1 AT5G03660 63.793 116 42 0 38 153 36 151 8.52e-40 132
MS.gene43075.t1 AT2G36410 66.279 86 29 0 38 123 57 142 3.19e-35 120
MS.gene43075.t1 AT5G03660 53.731 134 40 1 38 171 36 147 9.69e-32 112
MS.gene43075.t1 AT2G36355 62.651 83 31 0 91 173 1 83 1.15e-30 107
MS.gene43075.t1 AT2G36355 61.628 86 33 0 88 173 27 112 1.43e-30 107
MS.gene43075.t1 AT2G36355 44.144 111 55 3 22 130 5 110 3.07e-22 87.0

Find 30 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCTAACCTCCACTAATT+TGG 0.167435 2.4:-14235816 None:intergenic
AGAAATTGAGAGAAGAAAAT+TGG 0.229998 2.4:+14234459 MS.gene43075:CDS
ATTGCAACATCCTTCCTCTT+TGG 0.283049 2.4:-14235126 None:intergenic
TTTCATCTCTTCTTGGACTC+TGG 0.297684 2.4:-14234384 None:intergenic
GGGTCAAACCTGCCAGAAAA+AGG 0.317850 2.4:+14235194 MS.gene43075:CDS
GTGCAACATGTGAAGAATTC+AGG 0.351410 2.4:+14234346 MS.gene43075:CDS
TCTAGCAATGTTTAGAGAAA+AGG 0.359449 2.4:+14234432 MS.gene43075:CDS
TTCTGGCAGGTTTGACCCAA+TGG 0.366095 2.4:-14235189 None:intergenic
AAAATTGGAGGTTAGAGATA+AGG 0.419428 2.4:+14234474 MS.gene43075:CDS
GAGTCCAAGAAGAGATGAAA+AGG 0.433573 2.4:+14234387 MS.gene43075:CDS
AACAAAGAGTTGAAGCCATT+GGG 0.443322 2.4:+14235174 MS.gene43075:CDS
AGAAATTGAATAGGAAATTG+AGG 0.443344 2.4:+14235092 MS.gene43075:intron
GAGGATAAGGATGAAGAAGC+TGG 0.468797 2.4:+14235964 MS.gene43075:CDS
CGAAAAGGAAACAAAGAGAT+TGG 0.472358 2.4:+14234519 MS.gene43075:CDS
TCAACTTGTTACCAAATTAG+TGG 0.474598 2.4:+14235805 MS.gene43075:CDS
GAAATTGAATAGGAAATTGA+GGG 0.474638 2.4:+14235093 MS.gene43075:intron
TAACAAAGAGTTGAAGCCAT+TGG 0.493465 2.4:+14235173 MS.gene43075:CDS
GATAAGGTTCATGCTCATCT+TGG 0.506717 2.4:+14234490 MS.gene43075:CDS
TCATCTTGGTCGAGTCGAAA+AGG 0.517837 2.4:+14234504 MS.gene43075:CDS
TGACTGAAAGTGAGAGGATA+AGG 0.518680 2.4:+14235951 MS.gene43075:CDS
AAGAAGAGATGAAAAGGAAG+AGG 0.527146 2.4:+14234393 MS.gene43075:CDS
AATTGAGAGAAGAAAATTGG+AGG 0.531288 2.4:+14234462 MS.gene43075:CDS
AGAATACAATGAAGCTCTTG+AGG 0.569694 2.4:+14235754 MS.gene43075:CDS
GTTCACAGATCCAAAGAGGA+AGG 0.618763 2.4:+14235116 MS.gene43075:CDS
AGCTTGTGACTGAAAGTGAG+AGG 0.623994 2.4:+14235945 MS.gene43075:intron
ACTTGTTACCAAATTAGTGG+AGG 0.627774 2.4:+14235808 MS.gene43075:CDS
AGGGGTTCACAGATCCAAAG+AGG 0.671132 2.4:+14235112 MS.gene43075:CDS
AAATTGAATAGGAAATTGAG+GGG 0.691169 2.4:+14235094 MS.gene43075:intron
GATAAGGATGAAGAAGCTGG+AGG 0.694341 2.4:+14235967 MS.gene43075:CDS
AGTTAGAAAGAAGATAGACA+TGG 0.715757 2.4:+14235149 MS.gene43075:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATAAAATCAAATG+TGG + chr2.4:14234995-14235014 MS.gene43075:intron 10.0%
!!! TATTTTTTAAAAACAATGTA+CGG - chr2.4:14235649-14235668 None:intergenic 10.0%
!! ATATGTTTATTTAGTTGTTT+AGG + chr2.4:14235435-14235454 MS.gene43075:intron 15.0%
!! CAAACAAAGAATATTTATAA+TGG - chr2.4:14235072-14235091 None:intergenic 15.0%
!! GAAATTAAAGCAAATAATTA+AGG - chr2.4:14235371-14235390 None:intergenic 15.0%
!! TTATAATATCTTTACAACAT+AGG + chr2.4:14235264-14235283 MS.gene43075:intron 15.0%
!!! AATTATTTGCTTTAATTTCT+TGG + chr2.4:14235372-14235391 MS.gene43075:intron 15.0%
!!! TTGTGAAACTATATATTTTT+GGG + chr2.4:14235719-14235738 MS.gene43075:intron 15.0%
!! AAAAAAGAAATATGTTAGCT+AGG - chr2.4:14235892-14235911 None:intergenic 20.0%
!! ATTAAAGCAAATAATTAAGG+TGG - chr2.4:14235368-14235387 None:intergenic 20.0%
!! TTTGATATTGTAGAAAATCA+CGG + chr2.4:14234757-14234776 MS.gene43075:intron 20.0%
!!! CTTGTGAAACTATATATTTT+TGG + chr2.4:14235718-14235737 MS.gene43075:intron 20.0%
!!! CTTTTGATGACATAATATAT+GGG - chr2.4:14235399-14235418 None:intergenic 20.0%
!!! TGTGAAACTATATATTTTTG+GGG + chr2.4:14235720-14235739 MS.gene43075:intron 20.0%
! AAATTGAATAGGAAATTGAG+GGG + chr2.4:14235094-14235113 MS.gene43075:intron 25.0%
! AGAAATTGAATAGGAAATTG+AGG + chr2.4:14235092-14235111 MS.gene43075:intron 25.0%
! AGAAATTGAGAGAAGAAAAT+TGG + chr2.4:14234459-14234478 MS.gene43075:CDS 25.0%
! ATATCAAAAGCAAAACACAT+TGG - chr2.4:14235315-14235334 None:intergenic 25.0%
! ATGATGTTTCATTGTTTGTT+TGG + chr2.4:14234598-14234617 MS.gene43075:intron 25.0%
! CAAATTCAGTGTTTGTTTAT+TGG - chr2.4:14234565-14234584 None:intergenic 25.0%
! CAATGAGAAAAACAAAGAAA+AGG + chr2.4:14235781-14235800 MS.gene43075:CDS 25.0%
! CCATATATTATGTCATCAAA+AGG + chr2.4:14235397-14235416 MS.gene43075:intron 25.0%
! GAAATTGAATAGGAAATTGA+GGG + chr2.4:14235093-14235112 MS.gene43075:intron 25.0%
! TATATTATGTCATCAAAAGG+AGG + chr2.4:14235400-14235419 MS.gene43075:intron 25.0%
! TATCAAAAGCAAAACACATT+GGG - chr2.4:14235314-14235333 None:intergenic 25.0%
! TGTGCAAAGTTTCAAAATAA+CGG + chr2.4:14234704-14234723 MS.gene43075:intron 25.0%
! TTACATTTGATCTATAGTCA+AGG + chr2.4:14234911-14234930 MS.gene43075:intron 25.0%
!! CTTTGTTTGAGAAATTGAAT+AGG + chr2.4:14235083-14235102 MS.gene43075:intron 25.0%
!!! ATTGTTTGTTTGGTTTTTAC+TGG + chr2.4:14234608-14234627 MS.gene43075:intron 25.0%
!!! CCTTTTGATGACATAATATA+TGG - chr2.4:14235400-14235419 None:intergenic 25.0%
!!! TTCACTTGTTTGGTTTTTAT+TGG + chr2.4:14234661-14234680 MS.gene43075:intron 25.0%
AAAATTGGAGGTTAGAGATA+AGG + chr2.4:14234474-14234493 MS.gene43075:CDS 30.0%
AACAGTTAGTACTAAACAGT+TGG - chr2.4:14235229-14235248 None:intergenic 30.0%
AAGTTTCAAAATAACGGTCA+CGG + chr2.4:14234710-14234729 MS.gene43075:intron 30.0%
AATTGAGAGAAGAAAATTGG+AGG + chr2.4:14234462-14234481 MS.gene43075:CDS 30.0%
AATTTGAATTCCGTCTGTTT+CGG - chr2.4:14235572-14235591 None:intergenic 30.0%
ACAATATAACGTTGAAATCG+TGG + chr2.4:14234830-14234849 MS.gene43075:intron 30.0%
AGTTAGAAAGAAGATAGACA+TGG + chr2.4:14235149-14235168 MS.gene43075:CDS 30.0%
ATTTCTAACCTCCACTAATT+TGG - chr2.4:14235819-14235838 None:intergenic 30.0%
TAAAGCTAATCCTTAAATCC+TGG - chr2.4:14234894-14234913 None:intergenic 30.0%
TCTAGCAATGTTTAGAGAAA+AGG + chr2.4:14234432-14234451 MS.gene43075:CDS 30.0%
TTAAAAACAATGTACGGAAG+AGG - chr2.4:14235643-14235662 None:intergenic 30.0%
! CTTTGCATTATGTGAACTTT+TGG + chr2.4:14235919-14235938 MS.gene43075:intron 30.0%
! TACAACATAGGATTTGTTCA+AGG + chr2.4:14235276-14235295 MS.gene43075:intron 30.0%
! TGCTGATCATATGATCTTTT+TGG - chr2.4:14234310-14234329 None:intergenic 30.0%
! TGGAAAATCTAACCTTTTTC+TGG - chr2.4:14235209-14235228 None:intergenic 30.0%
!! TCAACTTGTTACCAAATTAG+TGG + chr2.4:14235805-14235824 MS.gene43075:CDS 30.0%
!! TTTTAAAAAACGGTCTGCAA+CGG - chr2.4:14234865-14234884 None:intergenic 30.0%
!!! CTATATATTTTTGGGGTTAC+AGG + chr2.4:14235727-14235746 MS.gene43075:intron 30.0%
!!! CTGGTCAAGGTTTTAAAAAA+CGG - chr2.4:14234875-14234894 None:intergenic 30.0%
AAAACCTTGACCAGGATTTA+AGG + chr2.4:14234881-14234900 MS.gene43075:intron 35.0%
AACAAAGAGTTGAAGCCATT+GGG + chr2.4:14235174-14235193 MS.gene43075:CDS 35.0%
AAGAAGAGATGAAAAGGAAG+AGG + chr2.4:14234393-14234412 MS.gene43075:CDS 35.0%
AGAATACAATGAAGCTCTTG+AGG + chr2.4:14235754-14235773 MS.gene43075:CDS 35.0%
AGACTAAAGTCTAAGCAATG+TGG - chr2.4:14235340-14235359 None:intergenic 35.0%
CAATATCAAAGATTACTGCG+TGG - chr2.4:14234747-14234766 None:intergenic 35.0%
CGAAAAGGAAACAAAGAGAT+TGG + chr2.4:14234519-14234538 MS.gene43075:CDS 35.0%
CGGAATTCAAATTTCTCAGT+TGG + chr2.4:14235579-14235598 MS.gene43075:intron 35.0%
TAACAAAGAGTTGAAGCCAT+TGG + chr2.4:14235173-14235192 MS.gene43075:CDS 35.0%
TAATCCTTAAATCCTGGTCA+AGG - chr2.4:14234888-14234907 None:intergenic 35.0%
TAGCGACCACAATTTGATAT+CGG + chr2.4:14234805-14234824 MS.gene43075:intron 35.0%
TGTGCAAAGTTTCACTTGTT+TGG + chr2.4:14234651-14234670 MS.gene43075:intron 35.0%
TTGTCACCGATATCAAATTG+TGG - chr2.4:14234814-14234833 None:intergenic 35.0%
! ACTTGTTACCAAATTAGTGG+AGG + chr2.4:14235808-14235827 MS.gene43075:CDS 35.0%
! TCTTCCTTTTCATCTCTTCT+TGG - chr2.4:14234394-14234413 None:intergenic 35.0%
!! AAATCTAACCTTTTTCTGGC+AGG - chr2.4:14235205-14235224 None:intergenic 35.0%
!! CTAACTAGCTTGTTTCATAC+AGG - chr2.4:14234964-14234983 None:intergenic 35.0%
!!! CGTTTTTTAAAACCTTGACC+AGG + chr2.4:14234873-14234892 MS.gene43075:intron 35.0%
ATTGCAACATCCTTCCTCTT+TGG - chr2.4:14235129-14235148 None:intergenic 40.0%
GACTTTGTAACCGAAACAGA+CGG + chr2.4:14235559-14235578 MS.gene43075:intron 40.0%
GAGTCCAAGAAGAGATGAAA+AGG + chr2.4:14234387-14234406 MS.gene43075:CDS 40.0%
GATAAGGTTCATGCTCATCT+TGG + chr2.4:14234490-14234509 MS.gene43075:CDS 40.0%
GTCAATCTCTTACCACCAAA+CGG - chr2.4:14235542-14235561 None:intergenic 40.0%
GTGCAACATGTGAAGAATTC+AGG + chr2.4:14234346-14234365 MS.gene43075:CDS 40.0%
TCAAATTGTGGTCGCTATTG+CGG - chr2.4:14234802-14234821 None:intergenic 40.0%
TGACTGAAAGTGAGAGGATA+AGG + chr2.4:14235951-14235970 MS.gene43075:CDS 40.0%
TTTCATCTCTTCTTGGACTC+TGG - chr2.4:14234387-14234406 None:intergenic 40.0%
AGCTTGTGACTGAAAGTGAG+AGG + chr2.4:14235945-14235964 MS.gene43075:intron 45.0%
ATCTCTTACCACCAAACGGA+AGG - chr2.4:14235538-14235557 None:intergenic 45.0%
GAGGATAAGGATGAAGAAGC+TGG + chr2.4:14235964-14235983 MS.gene43075:CDS 45.0%
GATAAGGATGAAGAAGCTGG+AGG + chr2.4:14235967-14235986 MS.gene43075:CDS 45.0%
GTTCACAGATCCAAAGAGGA+AGG + chr2.4:14235116-14235135 MS.gene43075:CDS 45.0%
TCATCTTGGTCGAGTCGAAA+AGG + chr2.4:14234504-14234523 MS.gene43075:CDS 45.0%
TGCTTCTTGTTCCTTCCGTT+TGG + chr2.4:14235524-14235543 MS.gene43075:intron 45.0%
TTCTTGTTCCTTCCGTTTGG+TGG + chr2.4:14235527-14235546 MS.gene43075:intron 45.0%
AGGGGTTCACAGATCCAAAG+AGG + chr2.4:14235112-14235131 MS.gene43075:CDS 50.0%
GGGTCAAACCTGCCAGAAAA+AGG + chr2.4:14235194-14235213 MS.gene43075:CDS 50.0%
TTCTGGCAGGTTTGACCCAA+TGG - chr2.4:14235192-14235211 None:intergenic 50.0%
! TAACGGTCACGGTCGCTTTG+CGG + chr2.4:14234721-14234740 MS.gene43075:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 14234298 14236021 14234298 ID=MS.gene43075
chr2.4 mRNA 14234298 14236021 14234298 ID=MS.gene43075.t1;Parent=MS.gene43075
chr2.4 exon 14234298 14234555 14234298 ID=MS.gene43075.t1.exon1;Parent=MS.gene43075.t1
chr2.4 CDS 14234298 14234555 14234298 ID=cds.MS.gene43075.t1;Parent=MS.gene43075.t1
chr2.4 exon 14235105 14235215 14235105 ID=MS.gene43075.t1.exon2;Parent=MS.gene43075.t1
chr2.4 CDS 14235105 14235215 14235105 ID=cds.MS.gene43075.t1;Parent=MS.gene43075.t1
chr2.4 exon 14235749 14235829 14235749 ID=MS.gene43075.t1.exon3;Parent=MS.gene43075.t1
chr2.4 CDS 14235749 14235829 14235749 ID=cds.MS.gene43075.t1;Parent=MS.gene43075.t1
chr2.4 exon 14235947 14236021 14235947 ID=MS.gene43075.t1.exon4;Parent=MS.gene43075.t1
chr2.4 CDS 14235947 14236021 14235947 ID=cds.MS.gene43075.t1;Parent=MS.gene43075.t1
Gene Sequence

>MS.gene43075

ATGGCAACACCAAAAAGATCATATGATCAGCAACAACAACTACAGATTGTGCAACATGTGAAGAATTCAGGAATGATTAACAACATCCAGAGTCCAAGAAGAGATGAAAAGGAAGAGGAAATGTCAAGATCAGTTCTAGCAATGTTTAGAGAAAAGGAAGAAGAAATTGAGAGAAGAAAATTGGAGGTTAGAGATAAGGTTCATGCTCATCTTGGTCGAGTCGAAAAGGAAACAAAGAGATTGGCTGAGATAAGAGAAGTAAGTCCAATAAACAAACACTGAATTTGAGCTAGATTGTTTATGATGTTTCATTGTTTGTTTGGTTTTTACTGGTTTATGCATTTATAAATCATTGTGCAAAGTTTCACTTGTTTGGTTTTTATTGGTTTATGTATTTATAAATCTTTGTGCAAAGTTTCAAAATAACGGTCACGGTCGCTTTGCGGCCACGCAGTAATCTTTGATATTGTAGAAAATCACGGATAAATACAACTTGTGTTGCCGCAATAGCGACCACAATTTGATATCGGTGACAATATAACGTTGAAATCGTGGCTATATTTGCCGTTGCAGACCGTTTTTTAAAACCTTGACCAGGATTTAAGGATTAGCTTTACATTTGATCTATAGTCAAGGAAAATTGTATTCACATGTTCATATATACCTGTATGAAACAAGCTAGTTAGAAACAATTTAAAAATAAAATAAAATCAAATGTGGTGTTTTTTTTTTTAAATTATTCACATTTTGTTATATTCAAGATTTTTTCATCCATTATAAATATTCTTTGTTTGAGAAATTGAATAGGAAATTGAGGGGTTCACAGATCCAAAGAGGAAGGATGTTGCAATAGTTAGAAAGAAGATAGACATGGTTAACAAAGAGTTGAAGCCATTGGGTCAAACCTGCCAGAAAAAGGTTAGATTTTCCAACTGTTTAGTACTAACTGTTGTTTGCTTTATTCTTTTATAATATCTTTACAACATAGGATTTGTTCAAGGAGACAAAGTGATCCCAATGTGTTTTGCTTTTGATATAACCACATTGCTTAGACTTTAGTCTATTGACCACCTTAATTATTTGCTTTAATTTCTTGGTCCCATATATTATGTCATCAAAAGGAGGCTATATAATACTATATGTTTATTTAGTTGTTTAGGTGATTATTCAAAATAATTGTGTTTGAATTTGCAAGTAGAAAATTGCTATTTGAAATTCGTCATACATGCTTCTTGTTCCTTCCGTTTGGTGGTAAGAGATTGACTTTGTAACCGAAACAGACGGAATTCAAATTTCTCAGTTGGAAAATGTCATTTTTGCAACTTTAATTTATGATAATTTTCCTCTTCCGTACATTGTTTTTAAAAAATAAATTAAAACAAGAATGAAAGTATGTTAACTATAAAAGTTTCTGAATCTTCTTGTGAAACTATATATTTTTGGGGTTACAGGAGAGAGAATACAATGAAGCTCTTGAGGCTTTCAATGAGAAAAACAAAGAAAAGGCTCAACTTGTTACCAAATTAGTGGAGGTTAGAAATTATTCATTGAATTGAATATTAGTACACTAATAATATCATATATTTTTATTCCTAGCTAACATATTTCTTTTTTTCTCTCTCTTTGCATTATGTGAACTTTTGGATTAGCTTGTGACTGAAAGTGAGAGGATAAGGATGAAGAAGCTGGAGGATCTAAGCAAAAATATAGATACACTTCACTAA

Protein sequence

>MS.gene43075.t1

MATPKRSYDQQQQLQIVQHVKNSGMINNIQSPRRDEKEEEMSRSVLAMFREKEEEIERRKLEVRDKVHAHLGRVEKETKRLAEIREEIEGFTDPKRKDVAIVRKKIDMVNKELKPLGQTCQKKEREYNEALEAFNEKNKEKAQLVTKLVELVTESERIRMKKLEDLSKNIDTLH