Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06756.t1 | AES72443.1 | 98.4 | 63 | 1 | 0 | 1 | 63 | 1 | 63 | 9.60E-22 | 112.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06756.t1 | Q8L9T8 | 63.5 | 63 | 23 | 0 | 1 | 63 | 1 | 63 | 1.1e-10 | 66.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06756.t1 | G7J699 | 98.4 | 63 | 1 | 0 | 1 | 63 | 1 | 63 | 6.9e-22 | 112.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04926 | MS.gene06756 | 0.803991 | 2.68E-49 | -1.69E-46 |
| MS.gene04932 | MS.gene06756 | 0.852772 | 3.71E-61 | -1.69E-46 |
| MS.gene049549 | MS.gene06756 | 0.812701 | 3.71E-51 | -1.69E-46 |
| MS.gene050133 | MS.gene06756 | 0.824966 | 6.06E-54 | -1.69E-46 |
| MS.gene050315 | MS.gene06756 | -0.800992 | 1.11E-48 | -1.69E-46 |
| MS.gene050329 | MS.gene06756 | 0.808079 | 3.69E-50 | -1.69E-46 |
| MS.gene050344 | MS.gene06756 | 0.811019 | 8.63E-51 | -1.69E-46 |
| MS.gene050369 | MS.gene06756 | 0.815911 | 7.25E-52 | -1.69E-46 |
| MS.gene050710 | MS.gene06756 | 0.827867 | 1.23E-54 | -1.69E-46 |
| MS.gene050720 | MS.gene06756 | 0.804584 | 2.01E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene06756 | 0.803709 | 3.06E-49 | -1.69E-46 |
| MS.gene050874 | MS.gene06756 | 0.811677 | 6.21E-51 | -1.69E-46 |
| MS.gene050875 | MS.gene06756 | 0.805111 | 1.56E-49 | -1.69E-46 |
| MS.gene051158 | MS.gene06756 | 0.807579 | 4.72E-50 | -1.69E-46 |
| MS.gene051385 | MS.gene06756 | 0.801117 | 1.05E-48 | -1.69E-46 |
| MS.gene051439 | MS.gene06756 | 0.845048 | 5.18E-59 | -1.69E-46 |
| MS.gene051552 | MS.gene06756 | 0.845394 | 4.17E-59 | -1.69E-46 |
| MS.gene051838 | MS.gene06756 | -0.803981 | 2.69E-49 | -1.69E-46 |
| MS.gene051973 | MS.gene06756 | 0.820601 | 6.29E-53 | -1.69E-46 |
| MS.gene051996 | MS.gene06756 | 0.850363 | 1.78E-60 | -1.69E-46 |
| MS.gene052011 | MS.gene06756 | 0.812971 | 3.24E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene06756 | 0.812062 | 5.12E-51 | -1.69E-46 |
| MS.gene052223 | MS.gene06756 | 0.855126 | 7.78E-62 | -1.69E-46 |
| MS.gene05240 | MS.gene06756 | 0.861585 | 9.25E-64 | -1.69E-46 |
| MS.gene052501 | MS.gene06756 | -0.806354 | 8.57E-50 | -1.69E-46 |
| MS.gene052505 | MS.gene06756 | -0.806782 | 6.96E-50 | -1.69E-46 |
| MS.gene052702 | MS.gene06756 | 0.817024 | 4.09E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene06756 | -0.805486 | 1.30E-49 | -1.69E-46 |
| MS.gene052825 | MS.gene06756 | -0.806172 | 9.36E-50 | -1.69E-46 |
| MS.gene053406 | MS.gene06756 | 0.818244 | 2.17E-52 | -1.69E-46 |
| MS.gene054107 | MS.gene06756 | -0.813056 | 3.11E-51 | -1.69E-46 |
| MS.gene054138 | MS.gene06756 | 0.827937 | 1.19E-54 | -1.69E-46 |
| MS.gene054147 | MS.gene06756 | 0.829042 | 6.42E-55 | -1.69E-46 |
| MS.gene054277 | MS.gene06756 | 0.869754 | 2.44E-66 | -1.69E-46 |
| MS.gene054706 | MS.gene06756 | 0.800167 | 1.64E-48 | -1.69E-46 |
| MS.gene054742 | MS.gene06756 | 0.892735 | 1.21E-74 | -1.69E-46 |
| MS.gene054884 | MS.gene06756 | 0.807859 | 4.11E-50 | -1.69E-46 |
| MS.gene055159 | MS.gene06756 | 0.80742 | 5.10E-50 | -1.69E-46 |
| MS.gene055215 | MS.gene06756 | 0.811421 | 7.06E-51 | -1.69E-46 |
| MS.gene055240 | MS.gene06756 | 0.817813 | 2.71E-52 | -1.69E-46 |
| MS.gene056076 | MS.gene06756 | 0.805199 | 1.50E-49 | -1.69E-46 |
| MS.gene056330 | MS.gene06756 | 0.873005 | 2.05E-67 | -1.69E-46 |
| MS.gene056514 | MS.gene06756 | -0.807041 | 6.14E-50 | -1.69E-46 |
| MS.gene056590 | MS.gene06756 | 0.830605 | 2.67E-55 | -1.69E-46 |
| MS.gene056591 | MS.gene06756 | 0.829142 | 6.07E-55 | -1.69E-46 |
| MS.gene056633 | MS.gene06756 | -0.80885 | 2.53E-50 | -1.69E-46 |
| MS.gene056680 | MS.gene06756 | 0.871136 | 8.60E-67 | -1.69E-46 |
| MS.gene056688 | MS.gene06756 | 0.822946 | 1.80E-53 | -1.69E-46 |
| MS.gene056690 | MS.gene06756 | 0.813797 | 2.13E-51 | -1.69E-46 |
| MS.gene056691 | MS.gene06756 | 0.818111 | 2.32E-52 | -1.69E-46 |
| MS.gene056697 | MS.gene06756 | 0.813762 | 2.17E-51 | -1.69E-46 |
| MS.gene056748 | MS.gene06756 | 0.804651 | 1.95E-49 | -1.69E-46 |
| MS.gene056920 | MS.gene06756 | 0.8835 | 4.26E-71 | -1.69E-46 |
| MS.gene056922 | MS.gene06756 | 0.842009 | 3.36E-58 | -1.69E-46 |
| MS.gene056923 | MS.gene06756 | 0.828566 | 8.37E-55 | -1.69E-46 |
| MS.gene056924 | MS.gene06756 | 0.82244 | 2.37E-53 | -1.69E-46 |
| MS.gene056925 | MS.gene06756 | 0.812846 | 3.45E-51 | -1.69E-46 |
| MS.gene056988 | MS.gene06756 | 0.812191 | 4.80E-51 | -1.69E-46 |
| MS.gene057048 | MS.gene06756 | -0.801109 | 1.05E-48 | -1.69E-46 |
| MS.gene057081 | MS.gene06756 | 0.800126 | 1.67E-48 | -1.69E-46 |
| MS.gene057261 | MS.gene06756 | 0.824493 | 7.83E-54 | -1.69E-46 |
| MS.gene057544 | MS.gene06756 | 0.833683 | 4.63E-56 | -1.69E-46 |
| MS.gene057717 | MS.gene06756 | 0.809261 | 2.07E-50 | -1.69E-46 |
| MS.gene058177 | MS.gene06756 | 0.809764 | 1.61E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene06756 | 0.830566 | 2.73E-55 | -1.69E-46 |
| MS.gene058418 | MS.gene06756 | 0.842338 | 2.75E-58 | -1.69E-46 |
| MS.gene058444 | MS.gene06756 | -0.8064 | 8.38E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene06756 | 0.809536 | 1.80E-50 | -1.69E-46 |
| MS.gene05926 | MS.gene06756 | 0.81852 | 1.88E-52 | -1.69E-46 |
| MS.gene059328 | MS.gene06756 | 0.821815 | 3.30E-53 | -1.69E-46 |
| MS.gene059512 | MS.gene06756 | -0.813713 | 2.23E-51 | -1.69E-46 |
| MS.gene059891 | MS.gene06756 | 0.855907 | 4.60E-62 | -1.69E-46 |
| MS.gene059892 | MS.gene06756 | 0.853718 | 1.99E-61 | -1.69E-46 |
| MS.gene059955 | MS.gene06756 | 0.802241 | 6.16E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene06756 | 0.904133 | 1.69E-79 | -1.69E-46 |
| MS.gene060358 | MS.gene06756 | 0.815432 | 9.27E-52 | -1.69E-46 |
| MS.gene060405 | MS.gene06756 | 0.800277 | 1.55E-48 | -1.69E-46 |
| MS.gene061038 | MS.gene06756 | 0.803415 | 3.52E-49 | -1.69E-46 |
| MS.gene061092 | MS.gene06756 | 0.829822 | 4.15E-55 | -1.69E-46 |
| MS.gene061094 | MS.gene06756 | 0.843617 | 1.26E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene55776 | MS.gene06756 | PPI |
| MS.gene048906 | MS.gene06756 | PPI |
| MS.gene06050 | MS.gene06756 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06756.t1 | MTR_3g090830 | 98.413 | 63 | 1 | 0 | 1 | 63 | 1 | 63 | 3.94e-38 | 120 |
| MS.gene06756.t1 | MTR_1g028610 | 76.271 | 59 | 12 | 1 | 7 | 63 | 4 | 62 | 2.29e-18 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06756.t1 | AT2G46330 | 63.462 | 52 | 19 | 0 | 12 | 63 | 11 | 62 | 9.80e-19 | 72.4 |
| MS.gene06756.t1 | AT3G61640 | 56.667 | 60 | 26 | 0 | 4 | 63 | 3 | 62 | 1.35e-17 | 69.3 |
| MS.gene06756.t1 | AT5G53250 | 58.182 | 55 | 23 | 0 | 9 | 63 | 9 | 63 | 2.23e-15 | 63.5 |
| MS.gene06756.t1 | AT5G24105 | 63.492 | 63 | 23 | 0 | 1 | 63 | 1 | 63 | 4.83e-11 | 52.4 |
Find 20 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTTTCATGTGCTTCATTC+AGG | 0.175879 | 3.3:+75901002 | MS.gene06756:CDS |
| TGTTCTAATGCTTGTGGCTT+TGG | 0.292333 | 3.3:+75901840 | MS.gene06756:CDS |
| TTCAGGGTGGTTGCCTTCCT+TGG | 0.296499 | 3.3:+75901019 | MS.gene06756:CDS |
| GATAAATTAATGAATGATAT+AGG | 0.396044 | 3.3:-75901869 | None:intergenic |
| GAATATTTACCATCGCTTGT+AGG | 0.408971 | 3.3:-75901103 | None:intergenic |
| TGTTTCATGTGCTTCATTCA+GGG | 0.448362 | 3.3:+75901003 | MS.gene06756:CDS |
| TTACCATCGCTTGTAGGAGC+AGG | 0.455603 | 3.3:-75901097 | None:intergenic |
| AGGAGCAGGGGCAGGTGCAA+TGG | 0.532358 | 3.3:-75901083 | None:intergenic |
| TTATGCAACACTTATTTCTG+TGG | 0.538882 | 3.3:+75901048 | MS.gene06756:CDS |
| TACCATCGCTTGTAGGAGCA+GGG | 0.542274 | 3.3:-75901096 | None:intergenic |
| AAATAAGTGTTGCATAAATG+AGG | 0.548157 | 3.3:-75901042 | None:intergenic |
| TGCATGTGTTCTAATGCTTG+TGG | 0.551000 | 3.3:+75901834 | MS.gene06756:CDS |
| TCGCTTGTAGGAGCAGGGGC+AGG | 0.566771 | 3.3:-75901091 | None:intergenic |
| TGCATAAATGAGGCCAAGGA+AGG | 0.567934 | 3.3:-75901032 | None:intergenic |
| TTAGAACACATGCAATCGCT+TGG | 0.574241 | 3.3:-75901825 | None:intergenic |
| TGCAATCGCTTGGTCAATTG+TGG | 0.581446 | 3.3:-75901815 | None:intergenic |
| GTGTTGCATAAATGAGGCCA+AGG | 0.582795 | 3.3:-75901036 | None:intergenic |
| GCCCCTGCTCCTACAAGCGA+TGG | 0.595947 | 3.3:+75901094 | MS.gene06756:CDS |
| ACCATCGCTTGTAGGAGCAG+GGG | 0.636251 | 3.3:-75901095 | None:intergenic |
| TTCATGTGCTTCATTCAGGG+TGG | 0.668781 | 3.3:+75901006 | MS.gene06756:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAAATTGAAAATCAAATA+TGG | - | chr3.3:75901703-75901722 | None:intergenic | 15.0% |
| !! | TAATGATTATAAAACAAATG+TGG | + | chr3.3:75901538-75901557 | MS.gene06756:intron | 15.0% |
| !! | TGAAAAATAAATTAAAATGG+AGG | - | chr3.3:75901219-75901238 | None:intergenic | 15.0% |
| !!! | AATCATTTCATAAATCTTTT+TGG | + | chr3.3:75901413-75901432 | MS.gene06756:intron | 15.0% |
| !!! | TTTATTTTCAGAGATTAAAT+TGG | - | chr3.3:75901259-75901278 | None:intergenic | 15.0% |
| !! | AAAGTGATGAAACATTAATA+AGG | - | chr3.3:75901159-75901178 | None:intergenic | 20.0% |
| !! | AAGTGATGAAACATTAATAA+GGG | - | chr3.3:75901158-75901177 | None:intergenic | 20.0% |
| !! | GCTAAAAGTTAAGTAAAATT+TGG | - | chr3.3:75901620-75901639 | None:intergenic | 20.0% |
| !!! | AAAACACCTTATACTTTTTA+AGG | + | chr3.3:75901455-75901474 | MS.gene06756:intron | 20.0% |
| !!! | AATTGTTGTTTATAATATGC+AGG | + | chr3.3:75901790-75901809 | MS.gene06756:intron | 20.0% |
| !!! | ATTTAGCCTTAAAAAGTATA+AGG | - | chr3.3:75901464-75901483 | None:intergenic | 20.0% |
| ! | TAATATCCATATAAACGGTT+AGG | + | chr3.3:75901563-75901582 | MS.gene06756:intron | 25.0% |
| ! | TCTTTATGAGCATTATTGAT+AGG | + | chr3.3:75901741-75901760 | MS.gene06756:intron | 25.0% |
| ! | TGGACTAATATCCATATAAA+CGG | + | chr3.3:75901558-75901577 | MS.gene06756:intron | 25.0% |
| !! | AAATAAGTGTTGCATAAATG+AGG | - | chr3.3:75901045-75901064 | None:intergenic | 25.0% |
| !! | ATCAAATATGGAGATGTTTT+TGG | - | chr3.3:75901691-75901710 | None:intergenic | 25.0% |
| !! | GATTAAATTGGTGATTCATT+TGG | - | chr3.3:75901247-75901266 | None:intergenic | 25.0% |
| !!! | AAACTCACATATATTTTGAG+AGG | - | chr3.3:75901496-75901515 | None:intergenic | 25.0% |
| !!! | CTTTCAATCACATTAGTTTT+CGG | - | chr3.3:75901384-75901403 | None:intergenic | 25.0% |
| GCTCATAAAGAATAATAGCA+TGG | - | chr3.3:75901732-75901751 | None:intergenic | 30.0% | |
| !! | TTATGCAACACTTATTTCTG+TGG | + | chr3.3:75901048-75901067 | MS.gene06756:CDS | 30.0% |
| GAATATTTACCATCGCTTGT+AGG | - | chr3.3:75901106-75901125 | None:intergenic | 35.0% | |
| GTTCTTCCTAACCGTTTATA+TGG | - | chr3.3:75901572-75901591 | None:intergenic | 35.0% | |
| TGTTTCATGTGCTTCATTCA+GGG | + | chr3.3:75901003-75901022 | MS.gene06756:CDS | 35.0% | |
| AAGATTGTTCCTACAAACCC+AGG | - | chr3.3:75901594-75901613 | None:intergenic | 40.0% | |
| ATAAACGGTTAGGAAGAACC+TGG | + | chr3.3:75901573-75901592 | MS.gene06756:intron | 40.0% | |
| CTGTTTCATGTGCTTCATTC+AGG | + | chr3.3:75901002-75901021 | MS.gene06756:CDS | 40.0% | |
| TAAACGGTTAGGAAGAACCT+GGG | + | chr3.3:75901574-75901593 | MS.gene06756:intron | 40.0% | |
| TCCCATTCCCTAGAACTAAA+GGG | + | chr3.3:75901659-75901678 | MS.gene06756:intron | 40.0% | |
| TGCATGTGTTCTAATGCTTG+TGG | + | chr3.3:75901834-75901853 | MS.gene06756:CDS | 40.0% | |
| TGTTCTAATGCTTGTGGCTT+TGG | + | chr3.3:75901840-75901859 | MS.gene06756:CDS | 40.0% | |
| TTAGAACACATGCAATCGCT+TGG | - | chr3.3:75901828-75901847 | None:intergenic | 40.0% | |
| !! | AACCCTTTAGTTCTAGGGAA+TGG | - | chr3.3:75901664-75901683 | None:intergenic | 40.0% |
| !! | ACCCTTTAGTTCTAGGGAAT+GGG | - | chr3.3:75901663-75901682 | None:intergenic | 40.0% |
| GTCCCATTCCCTAGAACTAA+AGG | + | chr3.3:75901658-75901677 | MS.gene06756:intron | 45.0% | |
| GTGTTGCATAAATGAGGCCA+AGG | - | chr3.3:75901039-75901058 | None:intergenic | 45.0% | |
| TAGGAAGAACCTGGGTTTGT+AGG | + | chr3.3:75901582-75901601 | MS.gene06756:intron | 45.0% | |
| TCCCTAGAACTAAAGGGTTG+TGG | + | chr3.3:75901665-75901684 | MS.gene06756:intron | 45.0% | |
| TGCAATCGCTTGGTCAATTG+TGG | - | chr3.3:75901818-75901837 | None:intergenic | 45.0% | |
| TGCATAAATGAGGCCAAGGA+AGG | - | chr3.3:75901035-75901054 | None:intergenic | 45.0% | |
| TTCATGTGCTTCATTCAGGG+TGG | + | chr3.3:75901006-75901025 | MS.gene06756:CDS | 45.0% | |
| !! | GCCACAACCCTTTAGTTCTA+GGG | - | chr3.3:75901669-75901688 | None:intergenic | 45.0% |
| !! | TAGTTCTAGGGAATGGGACA+TGG | - | chr3.3:75901657-75901676 | None:intergenic | 45.0% |
| !! | TATTGAAAAATAAATTAAAA+TGG | - | chr3.3:75901222-75901241 | None:intergenic | 5.0% |
| TACCATCGCTTGTAGGAGCA+GGG | - | chr3.3:75901099-75901118 | None:intergenic | 50.0% | |
| TTACCATCGCTTGTAGGAGC+AGG | - | chr3.3:75901100-75901119 | None:intergenic | 50.0% | |
| !! | GGCCACAACCCTTTAGTTCT+AGG | - | chr3.3:75901670-75901689 | None:intergenic | 50.0% |
| ACCATCGCTTGTAGGAGCAG+GGG | - | chr3.3:75901098-75901117 | None:intergenic | 55.0% | |
| TTCAGGGTGGTTGCCTTCCT+TGG | + | chr3.3:75901019-75901038 | MS.gene06756:CDS | 55.0% | |
| AGGAGCAGGGGCAGGTGCAA+TGG | - | chr3.3:75901086-75901105 | None:intergenic | 65.0% | |
| GCCCCTGCTCCTACAAGCGA+TGG | + | chr3.3:75901094-75901113 | MS.gene06756:CDS | 65.0% | |
| TCGCTTGTAGGAGCAGGGGC+AGG | - | chr3.3:75901094-75901113 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 75900998 | 75901885 | 75900998 | ID=MS.gene06756 |
| chr3.3 | mRNA | 75900998 | 75901885 | 75900998 | ID=MS.gene06756.t1;Parent=MS.gene06756 |
| chr3.3 | exon | 75900998 | 75901115 | 75900998 | ID=MS.gene06756.t1.exon1;Parent=MS.gene06756.t1 |
| chr3.3 | CDS | 75900998 | 75901115 | 75900998 | ID=cds.MS.gene06756.t1;Parent=MS.gene06756.t1 |
| chr3.3 | exon | 75901812 | 75901885 | 75901812 | ID=MS.gene06756.t1.exon2;Parent=MS.gene06756.t1 |
| chr3.3 | CDS | 75901812 | 75901885 | 75901812 | ID=cds.MS.gene06756.t1;Parent=MS.gene06756.t1 |
| Gene Sequence |
| Protein sequence |
