AlfalfaGEDB | MS.gene071074

M. sativa cultivar XinJiangDaYe / MS.gene071074

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008168 GO:0016740 GO:0016741
PFAM: PF03492.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071074.t1	XP_003614194.2	98.6	74	1	0	1	74	279	352	1.60E-31	144.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071074.t1	G7JWX2	98.6	74	1	0	1	74	339	412	1.1e-31	144.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene071074	0.819809	9.56E-53	-1.69E-46
MS.gene049653	MS.gene071074	0.816145	6.43E-52	-1.69E-46
MS.gene049821	MS.gene071074	0.80187	7.34E-49	-1.69E-46
MS.gene049983	MS.gene071074	0.820042	8.45E-53	-1.69E-46
MS.gene050437	MS.gene071074	0.818194	2.23E-52	-1.69E-46
MS.gene050646	MS.gene071074	0.813829	2.10E-51	-1.69E-46
MS.gene050654	MS.gene071074	0.807957	3.92E-50	-1.69E-46
MS.gene050689	MS.gene071074	0.825093	5.66E-54	-1.69E-46
MS.gene050752	MS.gene071074	0.837625	4.65E-57	-1.69E-46
MS.gene050955	MS.gene071074	0.840064	1.09E-57	-1.69E-46
MS.gene051003	MS.gene071074	0.832012	1.21E-55	-1.69E-46
MS.gene051243	MS.gene071074	0.81554	8.77E-52	-1.69E-46
MS.gene051343	MS.gene071074	0.841473	4.65E-58	-1.69E-46
MS.gene051363	MS.gene071074	0.821082	4.88E-53	-1.69E-46
MS.gene051365	MS.gene071074	0.814093	1.84E-51	-1.69E-46
MS.gene051597	MS.gene071074	0.809306	2.02E-50	-1.69E-46
MS.gene051856	MS.gene071074	0.829515	4.93E-55	-1.69E-46
MS.gene051975	MS.gene071074	0.81454	1.46E-51	-1.69E-46
MS.gene052072	MS.gene071074	0.805441	1.33E-49	-1.69E-46
MS.gene052108	MS.gene071074	0.804926	1.71E-49	-1.69E-46
MS.gene052516	MS.gene071074	0.800019	1.75E-48	-1.69E-46
MS.gene052954	MS.gene071074	0.824059	9.91E-54	-1.69E-46
MS.gene053008	MS.gene071074	0.802023	6.83E-49	-1.69E-46
MS.gene053058	MS.gene071074	0.823068	1.69E-53	-1.69E-46
MS.gene05364	MS.gene071074	0.829192	5.91E-55	-1.69E-46
MS.gene053931	MS.gene071074	0.828355	9.42E-55	-1.69E-46
MS.gene054403	MS.gene071074	0.835814	1.35E-56	-1.69E-46
MS.gene05517	MS.gene071074	0.827651	1.39E-54	-1.69E-46
MS.gene055513	MS.gene071074	0.804283	2.33E-49	-1.69E-46
MS.gene055623	MS.gene071074	0.836531	8.86E-57	-1.69E-46
MS.gene055841	MS.gene071074	0.830395	3.01E-55	-1.69E-46
MS.gene056480	MS.gene071074	0.821361	4.21E-53	-1.69E-46
MS.gene056699	MS.gene071074	0.829656	4.56E-55	-1.69E-46
MS.gene056776	MS.gene071074	0.809325	2.00E-50	-1.69E-46
MS.gene056787	MS.gene071074	0.820444	6.84E-53	-1.69E-46
MS.gene057034	MS.gene071074	0.836878	7.23E-57	-1.69E-46
MS.gene057102	MS.gene071074	0.801128	1.04E-48	-1.69E-46
MS.gene057208	MS.gene071074	0.858824	6.32E-63	-1.69E-46
MS.gene057371	MS.gene071074	0.819932	8.96E-53	-1.69E-46
MS.gene057392	MS.gene071074	0.839216	1.81E-57	-1.69E-46
MS.gene057694	MS.gene071074	0.812802	3.53E-51	-1.69E-46
MS.gene057705	MS.gene071074	0.815297	9.94E-52	-1.69E-46
MS.gene058008	MS.gene071074	0.82821	1.02E-54	-1.69E-46
MS.gene058009	MS.gene071074	0.814148	1.79E-51	-1.69E-46
MS.gene058126	MS.gene071074	0.807508	4.89E-50	-1.69E-46
MS.gene058261	MS.gene071074	0.80541	1.35E-49	-1.69E-46
MS.gene058783	MS.gene071074	0.815391	9.47E-52	-1.69E-46
MS.gene058976	MS.gene071074	0.835468	1.65E-56	-1.69E-46
MS.gene059146	MS.gene071074	0.814146	1.79E-51	-1.69E-46
MS.gene05985	MS.gene071074	-0.803046	4.20E-49	-1.69E-46
MS.gene059965	MS.gene071074	0.80551	1.29E-49	-1.69E-46
MS.gene060244	MS.gene071074	0.877179	7.73E-69	-1.69E-46
MS.gene060365	MS.gene071074	0.840085	1.08E-57	-1.69E-46
MS.gene060935	MS.gene071074	0.810855	9.37E-51	-1.69E-46
MS.gene060937	MS.gene071074	0.806354	8.57E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071074.t1	MTR_5g046110	98.649	74	1	0	1	74	339	412	1.04e-45	150

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071074.t1	AT4G36470	59.211	76	28	1	2	74	286	361	5.32e-23	89.7

Find 19 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCACTTTGGAGAAAAGATTT+TGG	0.182691	5.1:+34091136	MS.gene071074:CDS
GAAAGCAGGGTCATTGAAAT+TGG	0.298092	5.1:+34091007	MS.gene071074:CDS
GGTTAGAAATCATTGATTCT+TGG	0.390675	5.1:-34091115	None:intergenic
GTCATTGAAATTGGAGAGGT+TGG	0.423951	5.1:+34091016	MS.gene071074:CDS
TAGATAAGAAGGAGCAAGGC+AGG	0.435823	5.1:+34091051	MS.gene071074:CDS
TCAATGATTTCTAACCACTT+TGG	0.458721	5.1:+34091122	MS.gene071074:CDS
GTTTGAGAACTATGCAATGC+TGG	0.485835	5.1:+34091166	MS.gene071074:CDS
GAAGATGAAGTAATGAAAGC+AGG	0.486603	5.1:+34090993	None:intergenic
AATGTTTGACATAGATAAGA+AGG	0.500046	5.1:+34091040	MS.gene071074:CDS
AGAAATCATTGATTCTTGGA+TGG	0.518959	5.1:-34091111	None:intergenic
TGATGAAGAAATTGCAAAGG+AGG	0.543048	5.1:+34091190	MS.gene071074:CDS
GAGCAAGGCAGGGAGAGCTA+TGG	0.556285	5.1:+34091062	MS.gene071074:CDS
GACATAGATAAGAAGGAGCA+AGG	0.574472	5.1:+34091047	MS.gene071074:CDS
AGAACAAGAACAAACGTGAT+GGG	0.584403	5.1:-34091221	None:intergenic
AAGATGAAGTAATGAAAGCA+GGG	0.599911	5.1:+34090994	None:intergenic
CTATGGTACAGATGTTGCAA+AGG	0.624437	5.1:+34091079	MS.gene071074:CDS
AGATAAGAAGGAGCAAGGCA+GGG	0.631315	5.1:+34091052	MS.gene071074:CDS
CAGGGTCATTGAAATTGGAG+AGG	0.633479	5.1:+34091012	MS.gene071074:CDS
GGTTGATGAAGAAATTGCAA+AGG	0.715601	5.1:+34091187	MS.gene071074:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATGTTTGACATAGATAAGA+AGG	+	chr5.1:34091040-34091059	MS.gene071074:CDS	25.0%
	TCAATGATTTCTAACCACTT+TGG	+	chr5.1:34091122-34091141	MS.gene071074:CDS	30.0%
!!	AGAAATCATTGATTCTTGGA+TGG	-	chr5.1:34091114-34091133	None:intergenic	30.0%
!!	GGTTAGAAATCATTGATTCT+TGG	-	chr5.1:34091118-34091137	None:intergenic	30.0%
	TGATGAAGAAATTGCAAAGG+AGG	+	chr5.1:34091190-34091209	MS.gene071074:CDS	35.0%
!	CCAAAATCTTTTCTCCAAAG+TGG	-	chr5.1:34091139-34091158	None:intergenic	35.0%
!!	CCACTTTGGAGAAAAGATTT+TGG	+	chr5.1:34091136-34091155	MS.gene071074:CDS	35.0%
!!	GGTTGATGAAGAAATTGCAA+AGG	+	chr5.1:34091187-34091206	MS.gene071074:CDS	35.0%
	CTATGGTACAGATGTTGCAA+AGG	+	chr5.1:34091079-34091098	MS.gene071074:CDS	40.0%
	GAAAGCAGGGTCATTGAAAT+TGG	+	chr5.1:34091007-34091026	MS.gene071074:CDS	40.0%
	GACATAGATAAGAAGGAGCA+AGG	+	chr5.1:34091047-34091066	MS.gene071074:CDS	40.0%
	GTCATTGAAATTGGAGAGGT+TGG	+	chr5.1:34091016-34091035	MS.gene071074:CDS	40.0%
	GTTTGAGAACTATGCAATGC+TGG	+	chr5.1:34091166-34091185	MS.gene071074:CDS	40.0%
	AGATAAGAAGGAGCAAGGCA+GGG	+	chr5.1:34091052-34091071	MS.gene071074:CDS	45.0%
	CAGGGTCATTGAAATTGGAG+AGG	+	chr5.1:34091012-34091031	MS.gene071074:CDS	45.0%
	TAGATAAGAAGGAGCAAGGC+AGG	+	chr5.1:34091051-34091070	MS.gene071074:CDS	45.0%
	GAGCAAGGCAGGGAGAGCTA+TGG	+	chr5.1:34091062-34091081	MS.gene071074:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	34091005	34091228	34091005	ID=MS.gene071074
chr5.1	mRNA	34091005	34091228	34091005	ID=MS.gene071074.t1;Parent=MS.gene071074
chr5.1	exon	34091005	34091228	34091005	ID=MS.gene071074.t1.exon1;Parent=MS.gene071074.t1
chr5.1	CDS	34091005	34091228	34091005	ID=cds.MS.gene071074.t1;Parent=MS.gene071074.t1

Gene Sequence	>MS.gene071074 ATGAAAGCAGGGTCATTGAAATTGGAGAGGTTGGAAATGTTTGACATAGATAAGAAGGAGCAAGGCAGGGAGAGCTATGGTACAGATGTTGCAAAGGCAGTTAGAGCCATCCAAGAATCAATGATTTCTAACCACTTTGGAGAAAAGATTTTGGACAGTTTGTTTGAGAACTATGCAATGCTGGTTGATGAAGAAATTGCAAAGGAGGATATTAATCCCATCAC
Protein sequence	>MS.gene071074.t1 MKAGSLKLERLEMFDIDKKEQGRESYGTDVAKAVRAIQESMISNHFGEKILDSLFENYAMLVDEEIAKEDINPI