AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073123

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073123.t1 MTR_2g084535 94.986 359 18 0 1 359 1 359 0.0 712
MS.gene073123.t1 MTR_2g075300 57.022 356 137 7 6 359 3 344 1.07e-134 393
MS.gene073123.t1 MTR_7g105400 51.899 316 134 5 56 358 53 363 6.83e-103 312
MS.gene073123.t1 MTR_7g105390 50.158 317 145 4 56 359 48 364 5.62e-102 310
MS.gene073123.t1 MTR_7g105390 53.933 267 113 2 56 312 66 332 3.30e-94 289
MS.gene073123.t1 MTR_4g097590 46.316 285 140 3 56 331 56 336 3.36e-82 259
MS.gene073123.t1 MTR_5g021530 37.443 219 97 11 11 219 7 195 2.60e-24 103
MS.gene073123.t1 MTR_8g076210 38.636 176 89 7 55 223 13 176 7.74e-24 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073123.t1 AT5G22860 49.441 358 166 6 13 359 8 361 2.38e-114 337
MS.gene073123.t1 AT5G22860 49.300 357 166 6 13 358 8 360 2.24e-112 334
MS.gene073123.t1 AT5G22860 49.300 357 166 6 13 358 8 360 2.24e-112 334
MS.gene073123.t1 AT5G22860 49.300 357 166 6 13 358 8 360 4.09e-112 335
MS.gene073123.t1 AT5G22860 49.300 357 166 6 13 358 8 360 1.27e-111 335
MS.gene073123.t1 AT5G22860 49.300 357 166 6 13 358 8 360 1.47e-111 334
MS.gene073123.t1 AT5G22860 49.300 357 166 6 13 358 8 360 1.47e-111 334
MS.gene073123.t1 AT5G22860 49.300 357 166 6 13 358 8 360 1.47e-111 334
MS.gene073123.t1 AT2G24280 41.576 368 183 6 11 360 5 358 1.94e-90 278
MS.gene073123.t1 AT2G24280 41.689 367 182 6 11 359 5 357 4.81e-90 279
MS.gene073123.t1 AT5G65760 45.912 318 152 6 56 359 59 370 5.30e-90 280
MS.gene073123.t1 AT3G28680 42.286 175 96 4 190 360 18 191 1.72e-43 149
MS.gene073123.t1 AT4G36195 36.257 171 89 6 56 218 46 204 3.54e-20 92.0
MS.gene073123.t1 AT4G36195 36.257 171 89 6 56 218 46 204 3.54e-20 92.0
MS.gene073123.t1 AT4G36195 36.257 171 89 6 56 218 46 204 4.09e-20 91.7
MS.gene073123.t1 AT4G36190 37.427 171 87 7 56 218 46 204 2.37e-19 89.4
MS.gene073123.t1 AT2G18080 35.503 169 90 5 58 218 48 205 1.32e-18 87.4

Find 75 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGTTGCAATGTCGTTTA+AGG 0.140246 2.2:+13146642 None:intergenic
TCCTAAGAGTATAGGATTTA+TGG 0.181276 2.2:-13146431 MS.gene073123:CDS
ATTTGATCAATTTCAAATAT+TGG 0.182290 2.2:-13146519 MS.gene073123:CDS
TCACAATTACTGGGGAGTTT+TGG 0.211706 2.2:+13146095 None:intergenic
TTTGATCAATTTCAAATATT+GGG 0.216568 2.2:-13146518 MS.gene073123:CDS
TAAAATGTTTCAACATCTTT+TGG 0.224312 2.2:+13146607 None:intergenic
TTATATCAGCATCGGTATTA+TGG 0.238679 2.2:-13146247 MS.gene073123:intron
GGAATTGATAAAGCTTCTTT+TGG 0.245805 2.2:-13144714 MS.gene073123:CDS
TCACTTGGCCATTGGTGCTT+TGG 0.266851 2.2:-13145718 MS.gene073123:CDS
CAACTCAAAAGAATGGTTTA+AGG 0.287048 2.2:+13144818 None:intergenic
GATCCTCCAATCACAATTAC+TGG 0.319018 2.2:+13146085 None:intergenic
CAGTGCTTGCTGCATGGTTT+AGG 0.320590 2.2:-13145752 MS.gene073123:CDS
CCACTACATATCACGGTAAC+TGG 0.327485 2.2:+13144735 None:intergenic
GAACCATCTATTGCTTGTTC+TGG 0.339066 2.2:+13146454 None:intergenic
CTAAGAATTGAAAGACCATT+TGG 0.372278 2.2:+13145124 None:intergenic
GGACCGAAGTAGACAAATAT+TGG 0.373035 2.2:+13146475 None:intergenic
ACCATAAATCCTATACTCTT+AGG 0.386577 2.2:+13146430 None:intergenic
ATCCTTGATAAGATATATTC+AGG 0.388285 2.2:-13144684 MS.gene073123:CDS
ATGAAACAATATTGAACTCT+TGG 0.400378 2.2:-13145174 MS.gene073123:CDS
CTTAAACGACATTGCAACCT+TGG 0.412069 2.2:-13146641 MS.gene073123:CDS
TATGGAAAATCAATACCGTT+TGG 0.413960 2.2:-13146229 MS.gene073123:CDS
TTAAATGTATTATAGCTTTG+TGG 0.421207 2.2:+13146550 None:intergenic
AACTCAAAAGAATGGTTTAA+GGG 0.443392 2.2:+13144819 None:intergenic
TTCAGGTGTTGTGGCTTACA+AGG 0.449221 2.2:-13144667 MS.gene073123:CDS
AACTCCCCAGTAATTGTGAT+TGG 0.451013 2.2:-13146091 MS.gene073123:CDS
ACAACATCGAAATAAGCATT+TGG 0.451510 2.2:+13145654 None:intergenic
TATAGGATTTATGGTAGAAA+AGG 0.452220 2.2:-13146422 MS.gene073123:CDS
ATCACGGTAACTGGATAGTC+TGG 0.453975 2.2:+13144744 None:intergenic
GGTCCAGAACAAGCAATAGA+TGG 0.470136 2.2:-13146457 MS.gene073123:CDS
AATCAATACCGTTTGGATCA+AGG 0.470545 2.2:-13146222 MS.gene073123:CDS
ACTAAAAGAGCTTGAAAGGT+TGG 0.473092 2.2:+13146397 None:intergenic
TAGGAGTGGGATTGATAGAA+TGG 0.473688 2.2:+13146710 None:intergenic
GTTGTGGCTTACAAGGGAAA+TGG 0.475857 2.2:-13144660 MS.gene073123:CDS
GATGGTTCTCCTAAGAGTAT+AGG 0.476783 2.2:-13146439 MS.gene073123:CDS
ATCAATACCGTTTGGATCAA+GGG 0.480317 2.2:-13146221 MS.gene073123:CDS
AAATATCCTCACTTGGCCAT+TGG 0.510773 2.2:-13145726 MS.gene073123:CDS
TTCAAGAATGCAAACACACT+TGG 0.510819 2.2:-13146190 MS.gene073123:CDS
AGACGAGGCCAAAGCACCAA+TGG 0.515427 2.2:+13145710 None:intergenic
GCTCCAATATTTGTCTACTT+CGG 0.518528 2.2:-13146478 MS.gene073123:CDS
ATCAATTTCAAATATTGGGG+TGG 0.520871 2.2:-13146514 MS.gene073123:CDS
ATATCATCAAAGTAGAGGAT+TGG 0.521474 2.2:+13145684 None:intergenic
ATATACTAAAAGAGCTTGAA+AGG 0.523715 2.2:+13146393 None:intergenic
AAAGTAGCGTCGCAGCCAAA+TGG 0.523922 2.2:-13145139 MS.gene073123:CDS
TAAGATATATTCAGGTGTTG+TGG 0.531386 2.2:-13144676 MS.gene073123:CDS
CACCTGAATATATCTTATCA+AGG 0.532694 2.2:+13144682 None:intergenic
TTGATCAATTTCAAATATTG+GGG 0.533645 2.2:-13146517 MS.gene073123:CDS
ATCCTCCAATCACAATTACT+GGG 0.536985 2.2:+13146086 None:intergenic
CCTCTGACAACTCAAAAGAA+TGG 0.537469 2.2:+13144811 None:intergenic
ATCAATTCCACTACATATCA+CGG 0.544440 2.2:+13144728 None:intergenic
TGTCAATGAGTGTGTAGGAG+TGG 0.553790 2.2:+13146696 None:intergenic
TCCCCAGTAATTGTGATTGG+AGG 0.556726 2.2:-13146088 MS.gene073123:CDS
AGACAGAAGTGGGATGGAGA+TGG 0.556976 2.2:-13144602 MS.gene073123:CDS
TAGGCTCAAATATCCTCACT+TGG 0.561155 2.2:-13145733 MS.gene073123:CDS
CCAGTTACCGTGATATGTAG+TGG 0.564278 2.2:-13144735 MS.gene073123:CDS
ACCACTTCAAGTTGTTCACA+TGG 0.568293 2.2:-13146753 MS.gene073123:CDS
ATTGTGATTGGAGGATCATA+TGG 0.568935 2.2:-13146079 MS.gene073123:CDS
GCTTCTTCCCTTGATCCAAA+CGG 0.574524 2.2:+13146214 None:intergenic
AAATATATCTGAGACAGAAG+TGG 0.583630 2.2:-13144613 MS.gene073123:CDS
TCGTCGAATGGAGATAACCT+TGG 0.586295 2.2:+13146670 None:intergenic
GTCAATGAGTGTGTAGGAGT+GGG 0.593257 2.2:+13146697 None:intergenic
TATCTGAGACAGAAGTGGGA+TGG 0.594693 2.2:-13144608 MS.gene073123:CDS
TATACACAGTGCTTGCTGCA+TGG 0.596133 2.2:-13145758 MS.gene073123:intron
GTAGAGGATTGGAGCAGACG+AGG 0.605838 2.2:+13145695 None:intergenic
AGAAGTGGGATGGAGATGGC+AGG 0.608437 2.2:-13144598 MS.gene073123:CDS
AATATATCTGAGACAGAAGT+GGG 0.609429 2.2:-13144612 MS.gene073123:CDS
TCAGGTGTTGTGGCTTACAA+GGG 0.616951 2.2:-13144666 MS.gene073123:CDS
ATGTAATATCATCAAAGTAG+AGG 0.625566 2.2:+13145679 None:intergenic
ACTCAAAAGAATGGTTTAAG+GGG 0.631595 2.2:+13144820 None:intergenic
GCCATGTGAACAACTTGAAG+TGG 0.645854 2.2:+13146752 None:intergenic
GGTATTGTCAATGAGTGTGT+AGG 0.656725 2.2:+13146691 None:intergenic
AAAGCACCAATGGCCAAGTG+AGG 0.660296 2.2:+13145720 None:intergenic
GTGATTGGAGGATCATATGG+TGG 0.676251 2.2:-13146076 MS.gene073123:CDS
ACGGTAACTGGATAGTCTGG+AGG 0.692907 2.2:+13144747 None:intergenic
CACACTCATTGACAATACCA+AGG 0.695882 2.2:-13146687 MS.gene073123:CDS
TCCTCCAATCACAATTACTG+GGG 0.714063 2.2:+13146087 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTATATATTTGCA+GGG - chr2.2:13146055-13146074 MS.gene073123:intron 10.0%
!! AAAAACTAAATAAAAGAATA+CGG + chr2.2:13145520-13145539 None:intergenic 10.0%
!! TAAAAAAATTATATATTTGC+AGG - chr2.2:13146054-13146073 MS.gene073123:intron 10.0%
!!! AGAAAAAATTAGTTTATTTA+CGG + chr2.2:13145784-13145803 None:intergenic 10.0%
!! AAACAAAACAAAAAATTACA+AGG - chr2.2:13146017-13146036 MS.gene073123:intron 15.0%
!! AACGTTAAATTATTAAATGT+TGG - chr2.2:13146434-13146453 MS.gene073123:CDS 15.0%
!! ATTTGATCAATTTCAAATAT+TGG - chr2.2:13144831-13144850 MS.gene073123:intron 15.0%
!! TTTGATCAATTTCAAATATT+GGG - chr2.2:13144832-13144851 MS.gene073123:intron 15.0%
!!! AAAAAATTAGTTTATTTACG+GGG + chr2.2:13145782-13145801 None:intergenic 15.0%
!!! AAATCTATTCATTTTGAAAT+TGG + chr2.2:13145934-13145953 None:intergenic 15.0%
!!! CTAATTTTTTCTTTAAAACT+TGG - chr2.2:13145792-13145811 MS.gene073123:intron 15.0%
!!! GAAAAAATTAGTTTATTTAC+GGG + chr2.2:13145783-13145802 None:intergenic 15.0%
!!! TAAAGGTTTTTGAATTTTTT+AGG + chr2.2:13145456-13145475 None:intergenic 15.0%
!!! TAACTAATTAATTCATTTTC+AGG - chr2.2:13146135-13146154 MS.gene073123:CDS 15.0%
!!! TAATAATTTTATATCAGCAT+CGG - chr2.2:13145095-13145114 MS.gene073123:intron 15.0%
!!! TAATTTAACGTTTTTTTTTG+TGG + chr2.2:13146426-13146445 None:intergenic 15.0%
!! AAAAAAATATTGCTAGCAAA+GGG + chr2.2:13145016-13145035 None:intergenic 20.0%
!! CAATCTTAATCATAACATAA+AGG + chr2.2:13145473-13145492 None:intergenic 20.0%
!! TAAAAAAATATTGCTAGCAA+AGG + chr2.2:13145017-13145036 None:intergenic 20.0%
!! TGACTAAATCAAAACAATAA+TGG - chr2.2:13145959-13145978 MS.gene073123:intron 20.0%
!! TGGTAATATAAAACAAACAA+AGG + chr2.2:13146311-13146330 None:intergenic 20.0%
!! TTGATCAATTTCAAATATTG+GGG - chr2.2:13144833-13144852 MS.gene073123:intron 20.0%
!!! AATACAACACTTTTTGTTTT+TGG + chr2.2:13145985-13146004 None:intergenic 20.0%
!!! AATTTTAGTCCTTATTTTGA+GGG - chr2.2:13145876-13145895 MS.gene073123:intron 20.0%
!!! TAAAATGTTTCAACATCTTT+TGG + chr2.2:13144746-13144765 None:intergenic 20.0%
!!! TAATGAAAGTATGTTTATAC+TGG + chr2.2:13146270-13146289 None:intergenic 20.0%
!!! TAATTTTAGTCCTTATTTTG+AGG - chr2.2:13145875-13145894 MS.gene073123:intron 20.0%
!!! TTAAATGTATTATAGCTTTG+TGG + chr2.2:13144803-13144822 None:intergenic 20.0%
! AACTCAAAAGAATGGTTTAA+GGG + chr2.2:13146534-13146553 None:intergenic 25.0%
! AATGCATAATATGAAAAGTC+TGG - chr2.2:13146378-13146397 MS.gene073123:intron 25.0%
! AGGACTAATAATTTACTGTT+TGG + chr2.2:13146331-13146350 None:intergenic 25.0%
! ATATACTAAAAGAGCTTGAA+AGG + chr2.2:13144960-13144979 None:intergenic 25.0%
! ATCCTTGATAAGATATATTC+AGG - chr2.2:13146666-13146685 MS.gene073123:CDS 25.0%
! ATGAAACAATATTGAACTCT+TGG - chr2.2:13146176-13146195 MS.gene073123:CDS 25.0%
! ATGCATAATATGAAAAGTCT+GGG - chr2.2:13146379-13146398 MS.gene073123:intron 25.0%
! ATGTAATATCATCAAAGTAG+AGG + chr2.2:13145674-13145693 None:intergenic 25.0%
! CTAAAAGTTGTATGTTTACA+AGG + chr2.2:13145843-13145862 None:intergenic 25.0%
! TATAGGATTTATGGTAGAAA+AGG - chr2.2:13144928-13144947 MS.gene073123:intron 25.0%
! TATGATTAAGATTGTGATAG+TGG - chr2.2:13145479-13145498 MS.gene073123:intron 25.0%
!! AGCTCTTTTAGTATATATAG+AGG - chr2.2:13144964-13144983 MS.gene073123:intron 25.0%
!! TCTGTCTCAGATATATTTTT+GGG + chr2.2:13146735-13146754 None:intergenic 25.0%
!! TTCTGTCTCAGATATATTTT+TGG + chr2.2:13146736-13146755 None:intergenic 25.0%
!!! AATTTTAGTCCCTCAAAATA+AGG + chr2.2:13145888-13145907 None:intergenic 25.0%
!!! TTTAACGTTTTTTTTTGTGG+TGG + chr2.2:13146423-13146442 None:intergenic 25.0%
ACAACATCGAAATAAGCATT+TGG + chr2.2:13145699-13145718 None:intergenic 30.0%
ACCATAAATCCTATACTCTT+AGG + chr2.2:13144923-13144942 None:intergenic 30.0%
ACTCAAAAGAATGGTTTAAG+GGG + chr2.2:13146533-13146552 None:intergenic 30.0%
ATATCATCAAAGTAGAGGAT+TGG + chr2.2:13145669-13145688 None:intergenic 30.0%
ATCAATTCCACTACATATCA+CGG + chr2.2:13146625-13146644 None:intergenic 30.0%
ATCAATTTCAAATATTGGGG+TGG - chr2.2:13144836-13144855 MS.gene073123:intron 30.0%
CAACTCAAAAGAATGGTTTA+AGG + chr2.2:13146535-13146554 None:intergenic 30.0%
CACCTGAATATATCTTATCA+AGG + chr2.2:13146671-13146690 None:intergenic 30.0%
CTAAGAATTGAAAGACCATT+TGG + chr2.2:13146229-13146248 None:intergenic 30.0%
GAATGGATGAAAAATATGAG+AGG + chr2.2:13144626-13144645 None:intergenic 30.0%
TATGGAAAATCAATACCGTT+TGG - chr2.2:13145121-13145140 MS.gene073123:CDS 30.0%
TGCATAATATGAAAAGTCTG+GGG - chr2.2:13146380-13146399 MS.gene073123:intron 30.0%
TTACCATGTAACCTCAAATA+TGG - chr2.2:13145412-13145431 MS.gene073123:intron 30.0%
! AAATATATCTGAGACAGAAG+TGG - chr2.2:13146737-13146756 MS.gene073123:CDS 30.0%
! AATATATCTGAGACAGAAGT+GGG - chr2.2:13146738-13146757 MS.gene073123:CDS 30.0%
! GGAATTGATAAAGCTTCTTT+TGG - chr2.2:13146636-13146655 MS.gene073123:CDS 30.0%
! GGTTACATGGTAACATTTTA+TGG + chr2.2:13145405-13145424 None:intergenic 30.0%
! TAAGATATATTCAGGTGTTG+TGG - chr2.2:13146674-13146693 MS.gene073123:CDS 30.0%
! TCCTAAGAGTATAGGATTTA+TGG - chr2.2:13144919-13144938 MS.gene073123:intron 30.0%
!! CTGTCTCAGATATATTTTTG+GGG + chr2.2:13146734-13146753 None:intergenic 30.0%
!! TTATATCAGCATCGGTATTA+TGG - chr2.2:13145103-13145122 MS.gene073123:CDS 30.0%
!! TTCAACATCTTTTGGATTAG+TGG + chr2.2:13144738-13144757 None:intergenic 30.0%
!!! TTTAAGGTTTTTTCGTCGAA+TGG + chr2.2:13144695-13144714 None:intergenic 30.0%
AATCAATACCGTTTGGATCA+AGG - chr2.2:13145128-13145147 MS.gene073123:CDS 35.0%
ACTAAAAGAGCTTGAAAGGT+TGG + chr2.2:13144956-13144975 None:intergenic 35.0%
AGCAATCACAACCATATTTG+AGG + chr2.2:13145426-13145445 None:intergenic 35.0%
ATCAATACCGTTTGGATCAA+GGG - chr2.2:13145129-13145148 MS.gene073123:CDS 35.0%
ATCCTCCAATCACAATTACT+GGG + chr2.2:13145267-13145286 None:intergenic 35.0%
ATTGTGATTGGAGGATCATA+TGG - chr2.2:13145271-13145290 MS.gene073123:intron 35.0%
GCTCCAATATTTGTCTACTT+CGG - chr2.2:13144872-13144891 MS.gene073123:intron 35.0%
TGTCGCAATATTTGATGTTG+CGG - chr2.2:13145372-13145391 MS.gene073123:intron 35.0%
TTCAAGAATGCAAACACACT+TGG - chr2.2:13145160-13145179 MS.gene073123:CDS 35.0%
! CAACCATATTTGAGGTTACA+TGG + chr2.2:13145418-13145437 None:intergenic 35.0%
!! ACAACAAAACTCTAGAGTGT+TGG - chr2.2:13146466-13146485 MS.gene073123:CDS 35.0%
!!! TTTTGGATTAGTGGAAACCA+AGG + chr2.2:13144729-13144748 None:intergenic 35.0%
AAATATCCTCACTTGGCCAT+TGG - chr2.2:13145624-13145643 MS.gene073123:intron 40.0%
AACTCCCCAGTAATTGTGAT+TGG - chr2.2:13145259-13145278 MS.gene073123:intron 40.0%
CACACTCATTGACAATACCA+AGG - chr2.2:13144663-13144682 MS.gene073123:CDS 40.0%
CCTCTGACAACTCAAAAGAA+TGG + chr2.2:13146542-13146561 None:intergenic 40.0%
CTTAAACGACATTGCAACCT+TGG - chr2.2:13144709-13144728 MS.gene073123:CDS 40.0%
GAACCATCTATTGCTTGTTC+TGG + chr2.2:13144899-13144918 None:intergenic 40.0%
GATCCTCCAATCACAATTAC+TGG + chr2.2:13145268-13145287 None:intergenic 40.0%
GGACCGAAGTAGACAAATAT+TGG + chr2.2:13144878-13144897 None:intergenic 40.0%
GGTATTGTCAATGAGTGTGT+AGG + chr2.2:13144662-13144681 None:intergenic 40.0%
TAGGAGTGGGATTGATAGAA+TGG + chr2.2:13144643-13144662 None:intergenic 40.0%
TAGGCTCAAATATCCTCACT+TGG - chr2.2:13145617-13145636 MS.gene073123:intron 40.0%
TAGTCCTCTTGAGCTTAACT+CGG - chr2.2:13146344-13146363 MS.gene073123:intron 40.0%
TCCTCCAATCACAATTACTG+GGG + chr2.2:13145266-13145285 None:intergenic 40.0%
TGAGCTTAACTCGGTTGTTA+AGG - chr2.2:13146353-13146372 MS.gene073123:intron 40.0%
! CAAGGTTGCAATGTCGTTTA+AGG + chr2.2:13144711-13144730 None:intergenic 40.0%
! CACAATTACTGGGGAGTTTT+GGG + chr2.2:13145257-13145276 None:intergenic 40.0%
! GATGGTTCTCCTAAGAGTAT+AGG - chr2.2:13144911-13144930 MS.gene073123:intron 40.0%
! TCACAATTACTGGGGAGTTT+TGG + chr2.2:13145258-13145277 None:intergenic 40.0%
!! ACCACTTCAAGTTGTTCACA+TGG - chr2.2:13144597-13144616 MS.gene073123:CDS 40.0%
!!! CCATTCTTTTGAGTTGTCAG+AGG - chr2.2:13146539-13146558 MS.gene073123:CDS 40.0%
!!! GTTTTTTTTTGTGGTGGTCG+AGG + chr2.2:13146417-13146436 None:intergenic 40.0%
!!! TTTTGAGTTGTCAGAGGACT+TGG - chr2.2:13146545-13146564 MS.gene073123:CDS 40.0%
ACAACCGAGTTAAGCTCAAG+AGG + chr2.2:13146351-13146370 None:intergenic 45.0%
ATCACGGTAACTGGATAGTC+TGG + chr2.2:13146609-13146628 None:intergenic 45.0%
CCACTACATATCACGGTAAC+TGG + chr2.2:13146618-13146637 None:intergenic 45.0%
CCAGTTACCGTGATATGTAG+TGG - chr2.2:13146615-13146634 MS.gene073123:CDS 45.0%
GCCATGTGAACAACTTGAAG+TGG + chr2.2:13144601-13144620 None:intergenic 45.0%
GCTTCTTCCCTTGATCCAAA+CGG + chr2.2:13145139-13145158 None:intergenic 45.0%
GGTCCAGAACAAGCAATAGA+TGG - chr2.2:13144893-13144912 MS.gene073123:intron 45.0%
GTCAATGAGTGTGTAGGAGT+GGG + chr2.2:13144656-13144675 None:intergenic 45.0%
GTGATTGGAGGATCATATGG+TGG - chr2.2:13145274-13145293 MS.gene073123:intron 45.0%
GTTGTGGCTTACAAGGGAAA+TGG - chr2.2:13146690-13146709 MS.gene073123:CDS 45.0%
TCCCCAGTAATTGTGATTGG+AGG - chr2.2:13145262-13145281 MS.gene073123:intron 45.0%
TCGTCGAATGGAGATAACCT+TGG + chr2.2:13144683-13144702 None:intergenic 45.0%
TGTCAATGAGTGTGTAGGAG+TGG + chr2.2:13144657-13144676 None:intergenic 45.0%
! TATACACAGTGCTTGCTGCA+TGG - chr2.2:13145592-13145611 MS.gene073123:intron 45.0%
! TATCTGAGACAGAAGTGGGA+TGG - chr2.2:13146742-13146761 MS.gene073123:CDS 45.0%
! TCAGGTGTTGTGGCTTACAA+GGG - chr2.2:13146684-13146703 MS.gene073123:CDS 45.0%
! TTCAGGTGTTGTGGCTTACA+AGG - chr2.2:13146683-13146702 MS.gene073123:CDS 45.0%
!!! TTATTTTATTTAGAATAATT+TGG - chr2.2:13146099-13146118 MS.gene073123:CDS 5.0%
AAAGTAGCGTCGCAGCCAAA+TGG - chr2.2:13146211-13146230 MS.gene073123:CDS 50.0%
ACGGTAACTGGATAGTCTGG+AGG + chr2.2:13146606-13146625 None:intergenic 50.0%
AGACAGAAGTGGGATGGAGA+TGG - chr2.2:13146748-13146767 MS.gene073123:CDS 50.0%
! AAAGCACCAATGGCCAAGTG+AGG + chr2.2:13145633-13145652 None:intergenic 50.0%
! CAGTGCTTGCTGCATGGTTT+AGG - chr2.2:13145598-13145617 MS.gene073123:intron 50.0%
! TCACTTGGCCATTGGTGCTT+TGG - chr2.2:13145632-13145651 MS.gene073123:intron 50.0%
AGAAGTGGGATGGAGATGGC+AGG - chr2.2:13146752-13146771 MS.gene073123:CDS 55.0%
GTAGAGGATTGGAGCAGACG+AGG + chr2.2:13145658-13145677 None:intergenic 55.0%
! AGACGAGGCCAAAGCACCAA+TGG + chr2.2:13145643-13145662 None:intergenic 55.0%
GCGCGCACGCGTTTGTGTTT+GGG + chr2.2:13145335-13145354 None:intergenic 60.0%
TGCGCGCACGCGTTTGTGTT+TGG + chr2.2:13145336-13145355 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 13144590 13146782 13144590 ID=MS.gene073123
chr2.2 mRNA 13144590 13146782 13144590 ID=MS.gene073123.t1;Parent=MS.gene073123
chr2.2 exon 13146387 13146782 13146387 ID=MS.gene073123.t1.exon1;Parent=MS.gene073123.t1
chr2.2 CDS 13146387 13146782 13146387 ID=cds.MS.gene073123.t1;Parent=MS.gene073123.t1
chr2.2 exon 13146074 13146260 13146074 ID=MS.gene073123.t1.exon2;Parent=MS.gene073123.t1
chr2.2 CDS 13146074 13146260 13146074 ID=cds.MS.gene073123.t1;Parent=MS.gene073123.t1
chr2.2 exon 13145638 13145771 13145638 ID=MS.gene073123.t1.exon3;Parent=MS.gene073123.t1
chr2.2 CDS 13145638 13145771 13145638 ID=cds.MS.gene073123.t1;Parent=MS.gene073123.t1
chr2.2 exon 13145103 13145215 13145103 ID=MS.gene073123.t1.exon4;Parent=MS.gene073123.t1
chr2.2 CDS 13145103 13145215 13145103 ID=cds.MS.gene073123.t1;Parent=MS.gene073123.t1
chr2.2 exon 13144590 13144842 13144590 ID=MS.gene073123.t1.exon5;Parent=MS.gene073123.t1
chr2.2 CDS 13144590 13144842 13144590 ID=cds.MS.gene073123.t1;Parent=MS.gene073123.t1
Gene Sequence

>MS.gene073123

ATGAAACACCACTTCAAGTTGTTCACATGGCTTCCTCTCATATTTTTCATCCATTCTATCAATCCCACTCCTACACACTCATTGACAATACCAAGGTTATCTCCATTCGACGAAAAAACCTTAAACGACATTGCAACCTTGGTTTCCACTAATCCAAAAGATGTTGAAACATTTTACTACAAACAAGTTCTTGATCACTTCAACTATAATCCACAAAGCTATAATACATTTAATCAAAGATATTTGATCAATTTCAAATATTGGGGTGGTGCAAATTCTAGTGCTCCAATATTTGTCTACTTCGGTCCAGAACAAGCAATAGATGGTTCTCCTAAGAGTATAGGATTTATGGTAGAAAAGGCACCAACCTTTCAAGCTCTTTTAGTATATATAGAGGTACTCACTATGTTTCATTTTACTTTTCCCTTTGCTAGCAATATTTTTTTAACATTCAATTATCTAAAAAAAATTATCTAATTCAAATATTTTATTTGAGTTTACATATTAATAATTTTATATCAGCATCGGTATTATGGAAAATCAATACCGTTTGGATCAAGGGAAGAAGCGTTCAAGAATGCAAACACACTTGGATATTTTAACTCAGCTCAAGCACTTGCAGATTATGCAGAAGTGATCATAGACATAAAGAAGACATTGCATGCCCAAAACTCCCCAGTAATTGTGATTGGAGGATCATATGGTGGAAGTTAGTATCTTAAATTGTTTCTTTATACATACACCCAAACACAAACGCGTGCGCGCACACACATATATGTGTGTGTCGCAATATTTGATGTTGCGGAGAATTGCCATAAAATGTTACCATGTAACCTCAAATATGGTTGTGATTGCTTTGCACTCCTAAAAAATTCAAAAACCTTTATGTTATGATTAAGATTGTGATAGTGGACATTTTTTTTAGAACCGTATTCTTTTATTTAGTTTTTTTCTTCACATATATACATTGTTGTCTTGTTGAAATATCAATTTTTTGTTATATATACACAGTGCTTGCTGCATGGTTTAGGCTCAAATATCCTCACTTGGCCATTGGTGCTTTGGCCTCGTCTGCTCCAATCCTCTACTTTGATGATATTACATCACCAAATGCTTATTTCGATGTTGTTTCAAGAGATTTCAAAGTAACTTTACTTAGTTTCTTTTTTTTATTGAAATATGATTTAGTCCCCGTAAATAAACTAATTTTTTCTTTAAAACTTGGAAAAATATTTCTTTTTATTTTTAGTCCTTGTAAACATACAACTTTTAGTCTCTATAATATTAATTTTAGTCCTTATTTTGAGGGACTAAAATTAGATAGTCTACATATTTCAATGACCAATTTCAAAATGAATAGATTTTTCAATGACTAAATCAAAACAATAATGGCCAAAAACAAAAAGTGTTGTATTCACAAGAAGCAAAAACAAAACAAAAAATTACAAGGACTGAAAATAAAAATAAAAAAATTATATATTTGCAGGGATTAAAAATATATATAAACATTTATTTTATTTAGAATAATTTGGATGATTTCATGAGTAACTAATTAATTCATTTTCAGGAAGTCAGTGAGACATGTTATGAAACAATATTGAACTCTTGGTCTGAAATTGACAAAGTAGCGTCGCAGCCAAATGGTCTTTCAATTCTTAGTCAAAGATTCAACACTTGCCAGTATAAACATACTTTCATTACTTTTATTTTTTTTCATTCCTTTGTTTGTTTTATATTACCAAACAGTAAATTATTAGTCCTCTTGAGCTTAACTCGGTTGTTAAGGATAATGCATAATATGAAAAGTCTGGGGTTACGTTCAAACCTCGACCACCACAAAAAAAAACGTTAAATTATTAAATGTTGGATATTATATACAACAAAACTCTAGAGTGTTGGATGAAATTAAATGTATTGATGTTGTATACTTGATCTTGCAGCCCCTTAAACCATTCTTTTGAGTTGTCAGAGGACTTGGAAAGCATGTATACAAGTGCAGCTCAATATAATCATCCTCCAGACTATCCAGTTACCGTGATATGTAGTGGAATTGATAAAGCTTCTTTTGGAAATAATATCCTTGATAAGATATATTCAGGTGTTGTGGCTTACAAGGGAAATGGAACATGCAAAGTTAATAACCCCAAAAATATATCTGAGACAGAAGTGGGATGGAGATGGCAGGTAATTTGA
Protein sequence

>MS.gene073123.t1

MKHHFKLFTWLPLIFFIHSINPTPTHSLTIPRLSPFDEKTLNDIATLVSTNPKDVETFYYKQVLDHFNYNPQSYNTFNQRYLINFKYWGGANSSAPIFVYFGPEQAIDGSPKSIGFMVEKAPTFQALLVYIEHRYYGKSIPFGSREEAFKNANTLGYFNSAQALADYAEVIIDIKKTLHAQNSPVIVIGGSYGGMLAAWFRLKYPHLAIGALASSAPILYFDDITSPNAYFDVVSRDFKEVSETCYETILNSWSEIDKVASQPNGLSILSQRFNTCHPLNHSFELSEDLESMYTSAAQYNHPPDYPVTVICSGIDKASFGNNILDKIYSGVVAYKGNGTCKVNNPKNISETEVGWRWQVI