AlfalfaGEDB | MS.gene073827

M. sativa cultivar XinJiangDaYe / MS.gene073827

KEGG: K02918
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00831.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073827.t1	XP_004494985.1	97	99	3	0	1	99	1	99	1.50E-40	175.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073827.t1	Q9M5L0	91.9	99	8	0	1	99	1	99	4.8e-40	164.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073827.t1	A0A1S2XV31	97.0	99	3	0	1	99	1	99	1.1e-40	175.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049178	MS.gene073827	0.807996	3.85E-50	-1.69E-46
MS.gene049340	MS.gene073827	0.803682	3.10E-49	-1.69E-46
MS.gene049341	MS.gene073827	0.82643	2.72E-54	-1.69E-46
MS.gene049756	MS.gene073827	0.804242	2.37E-49	-1.69E-46
MS.gene049825	MS.gene073827	0.809809	1.57E-50	-1.69E-46
MS.gene049887	MS.gene073827	0.826723	2.32E-54	-1.69E-46
MS.gene050277	MS.gene073827	0.86998	2.06E-66	-1.69E-46
MS.gene050320	MS.gene073827	0.815707	8.05E-52	-1.69E-46
MS.gene050719	MS.gene073827	0.800602	1.33E-48	-1.69E-46
MS.gene050721	MS.gene073827	0.80022	1.60E-48	-1.69E-46
MS.gene050807	MS.gene073827	0.817566	3.09E-52	-1.69E-46
MS.gene050838	MS.gene073827	0.821462	3.99E-53	-1.69E-46
MS.gene051063	MS.gene073827	0.861806	7.92E-64	-1.69E-46
MS.gene051245	MS.gene073827	0.807411	5.12E-50	-1.69E-46
MS.gene051330	MS.gene073827	0.808853	2.53E-50	-1.69E-46
MS.gene051633	MS.gene073827	0.825812	3.82E-54	-1.69E-46
MS.gene051687	MS.gene073827	0.806253	9.00E-50	-1.69E-46
MS.gene051700	MS.gene073827	0.804336	2.27E-49	-1.69E-46
MS.gene051746	MS.gene073827	0.873948	9.89E-68	-1.69E-46
MS.gene051748	MS.gene073827	0.868042	8.76E-66	-1.69E-46
MS.gene051757	MS.gene073827	0.817557	3.10E-52	-1.69E-46
MS.gene051850	MS.gene073827	0.831102	2.02E-55	-1.69E-46
MS.gene051903	MS.gene073827	0.855318	6.84E-62	-1.69E-46
MS.gene052133	MS.gene073827	0.834367	3.12E-56	-1.69E-46
MS.gene052202	MS.gene073827	0.82159	3.72E-53	-1.69E-46
MS.gene052256	MS.gene073827	0.806881	6.63E-50	-1.69E-46
MS.gene052286	MS.gene073827	0.812387	4.35E-51	-1.69E-46
MS.gene052302	MS.gene073827	0.845152	4.85E-59	-1.69E-46
MS.gene052376	MS.gene073827	0.836498	9.03E-57	-1.69E-46
MS.gene052665	MS.gene073827	0.80997	1.45E-50	-1.69E-46
MS.gene052856	MS.gene073827	0.8291	6.22E-55	-1.69E-46
MS.gene052858	MS.gene073827	0.818738	1.68E-52	-1.69E-46
MS.gene052860	MS.gene073827	0.803665	3.13E-49	-1.69E-46
MS.gene052879	MS.gene073827	0.803658	3.14E-49	-1.69E-46
MS.gene052947	MS.gene073827	0.844462	7.45E-59	-1.69E-46
MS.gene053109	MS.gene073827	0.83091	2.25E-55	-1.69E-46
MS.gene053437	MS.gene073827	0.848957	4.40E-60	-1.69E-46
MS.gene053952	MS.gene073827	0.81803	2.43E-52	-1.69E-46
MS.gene055174	MS.gene073827	0.81245	4.21E-51	-1.69E-46
MS.gene055424	MS.gene073827	0.801289	9.66E-49	-1.69E-46
MS.gene055512	MS.gene073827	0.80179	7.62E-49	-1.69E-46
MS.gene055580	MS.gene073827	-0.805743	1.15E-49	-1.69E-46
MS.gene055591	MS.gene073827	-0.81305	3.11E-51	-1.69E-46
MS.gene055808	MS.gene073827	0.805977	1.03E-49	-1.69E-46
MS.gene056043	MS.gene073827	-0.819744	9.89E-53	-1.69E-46
MS.gene056069	MS.gene073827	-0.813149	2.96E-51	-1.69E-46
MS.gene056075	MS.gene073827	0.82378	1.15E-53	-1.69E-46
MS.gene056091	MS.gene073827	0.817575	3.07E-52	-1.69E-46
MS.gene056432	MS.gene073827	0.865405	6.05E-65	-1.69E-46
MS.gene056631	MS.gene073827	0.867619	1.20E-65	-1.69E-46
MS.gene056760	MS.gene073827	0.813253	2.81E-51	-1.69E-46
MS.gene057057	MS.gene073827	0.826376	2.81E-54	-1.69E-46
MS.gene057094	MS.gene073827	0.850912	1.25E-60	-1.69E-46
MS.gene057140	MS.gene073827	0.818151	2.28E-52	-1.69E-46
MS.gene057234	MS.gene073827	0.829909	3.95E-55	-1.69E-46
MS.gene058055	MS.gene073827	0.808977	2.38E-50	-1.69E-46
MS.gene058084	MS.gene073827	0.810263	1.26E-50	-1.69E-46
MS.gene05809	MS.gene073827	0.804514	2.08E-49	-1.69E-46
MS.gene058182	MS.gene073827	0.828828	7.24E-55	-1.69E-46
MS.gene058238	MS.gene073827	0.815922	7.21E-52	-1.69E-46
MS.gene058906	MS.gene073827	0.880647	4.62E-70	-1.69E-46
MS.gene059171	MS.gene073827	0.814789	1.29E-51	-1.69E-46
MS.gene059898	MS.gene073827	0.810082	1.38E-50	-1.69E-46
MS.gene06001	MS.gene073827	0.913878	3.69E-84	-1.69E-46
MS.gene060165	MS.gene073827	0.834322	3.20E-56	-1.69E-46
MS.gene060429	MS.gene073827	0.803073	4.15E-49	-1.69E-46
MS.gene060619	MS.gene073827	0.842386	2.67E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073827.t1	MTR_1g067490	95.833	96	4	0	4	99	3	98	1.89e-59	178
MS.gene073827.t1	MTR_4g062410	88.889	99	11	0	1	99	1	99	1.61e-56	171
MS.gene073827.t1	MTR_1g115545	92.708	96	7	0	4	99	2	97	2.05e-56	170
MS.gene073827.t1	MTR_7g080090	86.869	99	13	0	1	99	1	99	6.84e-54	164
MS.gene073827.t1	MTR_4g072410	85.417	96	14	0	5	100	3	98	2.56e-51	157
MS.gene073827.t1	MTR_1g105880	73.256	86	12	1	14	99	5	79	1.73e-34	114
MS.gene073827.t1	MTR_5g045540	87.879	33	4	0	67	99	2	34	4.02e-13	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073827.t1	AT5G02610	87.879	99	12	0	1	99	1	99	6.85e-55	166
MS.gene073827.t1	AT2G39390	87.000	100	13	0	1	100	1	100	1.61e-54	166
MS.gene073827.t1	AT3G09500	87.000	100	13	0	1	100	1	100	1.68e-54	166
MS.gene073827.t1	AT3G55170	86.000	100	14	0	1	100	1	100	7.03e-54	163
MS.gene073827.t1	AT3G55170	86.000	100	14	0	1	100	1	100	7.03e-54	163
MS.gene073827.t1	AT3G55170	85.000	100	15	0	1	100	1	100	6.85e-53	161
MS.gene073827.t1	AT3G55170	85.000	100	15	0	1	100	1	100	6.85e-53	161
MS.gene073827.t1	AT3G55170	85.000	100	15	0	1	100	1	100	6.85e-53	161
MS.gene073827.t1	AT5G02610	71.311	122	12	1	1	99	1	122	4.42e-49	152

Find 19 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCATTTGAAGTGAGAATTA+AGG	0.291537	1.3:-47415041	MS.gene073827:intron
CTGATAGCACGGGTCTTCTT+AGG	0.307833	1.3:+47414692	None:intergenic
CTGGTTCAGCAAATCAGCTT+TGG	0.322482	1.3:+47414997	None:intergenic
TGGTTCAGCAAATCAGCTTT+GGG	0.386643	1.3:+47414998	None:intergenic
AGGTGCACCGCCGGTGACTT+TGG	0.448506	1.3:+47414934	None:intergenic
ATTAAACCTGGTGTTTGGTA+AGG	0.478429	1.3:+47414663	None:intergenic
CCGCCTTCAGATCCTTCAGC+TGG	0.492136	1.3:+47414978	None:intergenic
CTTCGAGTCGCCAAAGTCAC+CGG	0.499663	1.3:-47414944	MS.gene073827:CDS
GAGCTTGTTAGGTGCACCGC+CGG	0.541755	1.3:+47414925	None:intergenic
GAATTAAGGTGTTCGAGTTG+AGG	0.547821	1.3:-47415027	MS.gene073827:CDS
TGATTTGCTGAACCAGCTGA+AGG	0.570604	1.3:-47414990	MS.gene073827:CDS
GAACCAGCTGAAGGATCTGA+AGG	0.580944	1.3:-47414981	MS.gene073827:CDS
TAAGGCGTCTTCTGATAGCA+CGG	0.582904	1.3:+47414681	None:intergenic
TTCTTAGGACGAAGATCAAG+TGG	0.602701	1.3:+47414707	None:intergenic
AAGGCGTCTTCTGATAGCAC+GGG	0.605672	1.3:+47414682	None:intergenic
AAGACGCCTTACCAAACACC+AGG	0.624051	1.3:-47414669	MS.gene073827:CDS
CGAGTCGCCAAAGTCACCGG+CGG	0.675842	1.3:-47414941	MS.gene073827:CDS
CCAGCTGAAGGATCTGAAGG+CGG	0.695519	1.3:-47414978	MS.gene073827:CDS
GCAGAAAGCTGCGCTCAGAG+AGG	0.696591	1.3:-47414753	MS.gene073827:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTATGTATTGTTTACATTT+TGG	+	chr1.3:47414898-47414917	None:intergenic	15.0%
!!!	AATACATAATCGCTTTTATA+TGG	-	chr1.3:47414908-47414927	MS.gene073827:intron	20.0%
	AATTCTCACTTCAAATGCAA+TGG	+	chr1.3:47414754-47414773	None:intergenic	30.0%
	TTATATGGAAGCTGCTTATA+TGG	-	chr1.3:47414923-47414942	MS.gene073827:CDS	30.0%
!	TGCATTTGAAGTGAGAATTA+AGG	-	chr1.3:47414755-47414774	MS.gene073827:CDS	30.0%
!!!	TTTTTTACAGCAAAGTTGTG+AGG	-	chr1.3:47414982-47415001	MS.gene073827:CDS	30.0%
!!	TACATTTTGGAGAGCTTGTT+AGG	+	chr1.3:47414885-47414904	None:intergenic	35.0%
!!	TGGTAATCGCGTTTTGTTAA+TGG	-	chr1.3:47414943-47414962	MS.gene073827:CDS	35.0%
	TGGTTCAGCAAATCAGCTTT+GGG	+	chr1.3:47414801-47414820	None:intergenic	40.0%
	TTCTTAGGACGAAGATCAAG+TGG	+	chr1.3:47415092-47415111	None:intergenic	40.0%
!!	GAATTAAGGTGTTCGAGTTG+AGG	-	chr1.3:47414769-47414788	MS.gene073827:CDS	40.0%
	CTGGTTCAGCAAATCAGCTT+TGG	+	chr1.3:47414802-47414821	None:intergenic	45.0%
!	TAAGGCGTCTTCTGATAGCA+CGG	+	chr1.3:47415118-47415137	None:intergenic	45.0%
!!	TGATTTGCTGAACCAGCTGA+AGG	-	chr1.3:47414806-47414825	MS.gene073827:CDS	45.0%
	AAGACGCCTTACCAAACACC+AGG	-	chr1.3:47415127-47415146	MS.gene073827:intron	50.0%
	CTGATAGCACGGGTCTTCTT+AGG	+	chr1.3:47415107-47415126	None:intergenic	50.0%
	GAACCAGCTGAAGGATCTGA+AGG	-	chr1.3:47414815-47414834	MS.gene073827:intron	50.0%
!	AAGGCGTCTTCTGATAGCAC+GGG	+	chr1.3:47415117-47415136	None:intergenic	50.0%
	CCAGCTGAAGGATCTGAAGG+CGG	-	chr1.3:47414818-47414837	MS.gene073827:intron	55.0%
	CTTCGAGTCGCCAAAGTCAC+CGG	-	chr1.3:47414852-47414871	MS.gene073827:intron	55.0%
	CCGCCTTCAGATCCTTCAGC+TGG	+	chr1.3:47414821-47414840	None:intergenic	60.0%
	GCAGAAAGCTGCGCTCAGAG+AGG	-	chr1.3:47415043-47415062	MS.gene073827:intron	60.0%
!!	GAGCTTGTTAGGTGCACCGC+CGG	+	chr1.3:47414874-47414893	None:intergenic	60.0%
	AGGTGCACCGCCGGTGACTT+TGG	+	chr1.3:47414865-47414884	None:intergenic	65.0%
	CGAGTCGCCAAAGTCACCGG+CGG	-	chr1.3:47414855-47414874	MS.gene073827:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	47414664	47415154	47414664	ID=MS.gene073827
chr1.3	mRNA	47414664	47415154	47414664	ID=MS.gene073827.t1;Parent=MS.gene073827
chr1.3	exon	47415151	47415154	47415151	ID=MS.gene073827.t1.exon1;Parent=MS.gene073827.t1
chr1.3	CDS	47415151	47415154	47415151	ID=cds.MS.gene073827.t1;Parent=MS.gene073827.t1
chr1.3	exon	47414917	47415052	47414917	ID=MS.gene073827.t1.exon2;Parent=MS.gene073827.t1
chr1.3	CDS	47414917	47415052	47414917	ID=cds.MS.gene073827.t1;Parent=MS.gene073827.t1
chr1.3	exon	47414664	47414826	47414664	ID=MS.gene073827.t1.exon3;Parent=MS.gene073827.t1
chr1.3	CDS	47414664	47414826	47414664	ID=cds.MS.gene073827.t1;Parent=MS.gene073827.t1

Gene Sequence

>MS.gene073827

ATGGGTAAGAATGTGAATTTAGTTTCAGTAACGATTTAATCAAATCAAATTTGTAACATTTTTCAACATATAAGCTGAATTGAATTTCCATTGCATTTGAAGTGAGAATTAAGGTGTTCGAGTTGAGGAACAAACCCAAAGCTGATTTGCTGAACCAGCTGAAGGATCTGAAGGCGGAGCTCGCTTTGCTTCGAGTCGCCAAAGTCACCGGCGGTGCACCTAACAAGCTCTCCAAAATGTAAACAATACATAATCGCTTTTATATGGAAGCTGCTTATATGGTAATCGCGTTTTGTTAATGGTGTTTTTGTTTGTTTGTTTTTTACAGCAAAGTTGTGAGGCTTTCAATTGCACAAGTGTTGACAGTGATTTCACAGAAGCAGAAAGCTGCGCTCAGAGAGGTTTACAAAAATAAGAAGTATTTACCACTTGATCTTCGTCCTAAGAAGACCCGTGCTATCAGAAGACGCCTTACCAAACACCAGGTTTAA

Protein sequence

>MS.gene073827.t1

MVRIKVFELRNKPKADLLNQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQV