AlfalfaGEDB | MS.gene08618

M. sativa cultivar XinJiangDaYe / MS.gene08618

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046982 GO:0046983
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08618.t1	XP_003606405.1	100	63	0	0	89	151	79	141	2.70E-26	128.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08618.t1	G7JPE7	100.0	63	0	0	89	151	79	141	2.0e-26	128.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene08618	0.84089	6.62E-58	-1.69E-46
MS.gene049501	MS.gene08618	0.807828	4.18E-50	-1.69E-46
MS.gene049666	MS.gene08618	0.823288	1.50E-53	-1.69E-46
MS.gene049825	MS.gene08618	0.827479	1.53E-54	-1.69E-46
MS.gene049873	MS.gene08618	0.831954	1.25E-55	-1.69E-46
MS.gene050026	MS.gene08618	0.809754	1.62E-50	-1.69E-46
MS.gene050156	MS.gene08618	0.809098	2.24E-50	-1.69E-46
MS.gene050201	MS.gene08618	0.80277	4.79E-49	-1.69E-46
MS.gene050303	MS.gene08618	0.82234	2.50E-53	-1.69E-46
MS.gene050355	MS.gene08618	0.826942	2.06E-54	-1.69E-46
MS.gene050442	MS.gene08618	0.822927	1.82E-53	-1.69E-46
MS.gene050719	MS.gene08618	0.829845	4.10E-55	-1.69E-46
MS.gene050721	MS.gene08618	0.828246	1.00E-54	-1.69E-46
MS.gene050724	MS.gene08618	0.812709	3.70E-51	-1.69E-46
MS.gene050750	MS.gene08618	0.806664	7.37E-50	-1.69E-46
MS.gene050844	MS.gene08618	0.815578	8.60E-52	-1.69E-46
MS.gene050953	MS.gene08618	0.813812	2.12E-51	-1.69E-46
MS.gene051247	MS.gene08618	0.835736	1.41E-56	-1.69E-46
MS.gene051265	MS.gene08618	0.802908	4.49E-49	-1.69E-46
MS.gene051594	MS.gene08618	0.849052	4.14E-60	-1.69E-46
MS.gene051700	MS.gene08618	0.805649	1.21E-49	-1.69E-46
MS.gene051744	MS.gene08618	0.815785	7.74E-52	-1.69E-46
MS.gene052020	MS.gene08618	0.812402	4.32E-51	-1.69E-46
MS.gene052190	MS.gene08618	0.807616	4.63E-50	-1.69E-46
MS.gene052208	MS.gene08618	0.804397	2.20E-49	-1.69E-46
MS.gene052519	MS.gene08618	0.836952	6.92E-57	-1.69E-46
MS.gene052561	MS.gene08618	0.816633	5.00E-52	-1.69E-46
MS.gene052582	MS.gene08618	0.818733	1.68E-52	-1.69E-46
MS.gene052584	MS.gene08618	0.826123	3.22E-54	-1.69E-46
MS.gene052773	MS.gene08618	0.802773	4.78E-49	-1.69E-46
MS.gene052867	MS.gene08618	0.801931	7.13E-49	-1.69E-46
MS.gene053006	MS.gene08618	0.826297	2.93E-54	-1.69E-46
MS.gene053054	MS.gene08618	-0.830503	2.83E-55	-1.69E-46
MS.gene053221	MS.gene08618	0.815262	1.01E-51	-1.69E-46
MS.gene053226	MS.gene08618	0.850027	2.21E-60	-1.69E-46
MS.gene05365	MS.gene08618	0.825446	4.67E-54	-1.69E-46
MS.gene053883	MS.gene08618	0.82259	2.18E-53	-1.69E-46
MS.gene053900	MS.gene08618	0.801607	8.31E-49	-1.69E-46
MS.gene053998	MS.gene08618	0.805001	1.65E-49	-1.69E-46
MS.gene05414	MS.gene08618	0.802103	6.57E-49	-1.69E-46
MS.gene05415	MS.gene08618	0.812303	4.54E-51	-1.69E-46
MS.gene054291	MS.gene08618	-0.805626	1.22E-49	-1.69E-46
MS.gene054405	MS.gene08618	0.806945	6.43E-50	-1.69E-46
MS.gene054651	MS.gene08618	0.823919	1.07E-53	-1.69E-46
MS.gene054775	MS.gene08618	0.811988	5.32E-51	-1.69E-46
MS.gene055357	MS.gene08618	0.810898	9.17E-51	-1.69E-46
MS.gene055466	MS.gene08618	0.814922	1.20E-51	-1.69E-46
MS.gene055778	MS.gene08618	0.856962	2.26E-62	-1.69E-46
MS.gene055808	MS.gene08618	0.817806	2.72E-52	-1.69E-46
MS.gene055818	MS.gene08618	0.847541	1.08E-59	-1.69E-46
MS.gene056091	MS.gene08618	0.816068	6.69E-52	-1.69E-46
MS.gene056162	MS.gene08618	0.810664	1.03E-50	-1.69E-46
MS.gene056174	MS.gene08618	0.818272	2.14E-52	-1.69E-46
MS.gene056373	MS.gene08618	0.826787	2.24E-54	-1.69E-46
MS.gene056498	MS.gene08618	0.840842	6.82E-58	-1.69E-46
MS.gene056506	MS.gene08618	0.818019	2.44E-52	-1.69E-46
MS.gene056595	MS.gene08618	0.814227	1.72E-51	-1.69E-46
MS.gene057060	MS.gene08618	0.829912	3.95E-55	-1.69E-46
MS.gene057314	MS.gene08618	0.86269	4.24E-64	-1.69E-46
MS.gene057414	MS.gene08618	0.809636	1.72E-50	-1.69E-46
MS.gene057693	MS.gene08618	0.819005	1.46E-52	-1.69E-46
MS.gene058037	MS.gene08618	0.827711	1.35E-54	-1.69E-46
MS.gene058084	MS.gene08618	0.819516	1.11E-52	-1.69E-46
MS.gene058625	MS.gene08618	0.831758	1.39E-55	-1.69E-46
MS.gene058979	MS.gene08618	0.807394	5.16E-50	-1.69E-46
MS.gene059084	MS.gene08618	0.806797	6.91E-50	-1.69E-46
MS.gene05954	MS.gene08618	0.857412	1.66E-62	-1.69E-46
MS.gene059569	MS.gene08618	0.805625	1.22E-49	-1.69E-46
MS.gene059625	MS.gene08618	0.845837	3.17E-59	-1.69E-46
MS.gene060322	MS.gene08618	0.807724	4.39E-50	-1.69E-46
MS.gene060429	MS.gene08618	0.854193	1.45E-61	-1.69E-46
MS.gene060521	MS.gene08618	0.824639	7.24E-54	-1.69E-46
MS.gene060553	MS.gene08618	0.81561	8.47E-52	-1.69E-46
MS.gene060572	MS.gene08618	0.832586	8.69E-56	-1.69E-46
MS.gene060587	MS.gene08618	0.806041	9.97E-50	-1.69E-46
MS.gene060634	MS.gene08618	-0.816599	5.09E-52	-1.69E-46
MS.gene060907	MS.gene08618	0.801234	9.91E-49	-1.69E-46
MS.gene060917	MS.gene08618	0.801429	9.04E-49	-1.69E-46
MS.gene061040	MS.gene08618	-0.840384	8.98E-58	-1.69E-46
MS.gene061266	MS.gene08618	0.807654	4.55E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08618.t1	MTR_4g059710	82.781	151	16	1	1	151	1	141	3.81e-78	230

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08618.t1	AT1G07980	42.254	71	40	1	81	151	99	168	3.82e-14	67.0
MS.gene08618.t1	AT1G07980	42.466	73	41	1	81	153	99	170	5.40e-14	67.4

Find 27 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCACTTTCTTCCTGTTCTT+TGG	0.144244	4.1:+39012937	None:intergenic
TTCGTTCTTTATCTTCTCTT+TGG	0.213367	4.1:+39013021	None:intergenic
TGTTTCTCAGGAAGCTATTT+TGG	0.232045	4.1:-39012804	MS.gene08618:CDS
TTCTTCCTGTTCTTTGGAAT+TGG	0.238672	4.1:+39012943	None:intergenic
TGTTTCCTATGAATCGAATT+AGG	0.259220	4.1:-39012859	MS.gene08618:CDS
GTTGCGTTTAAATTATTATT+CGG	0.364741	4.1:-39012481	MS.gene08618:CDS
CAATTAGCGGAGGATGCTTA+TGG	0.368797	4.1:-39012654	MS.gene08618:CDS
TAGCGATTTGCGTGTTTCTC+AGG	0.418237	4.1:-39012816	MS.gene08618:CDS
TGCTTATGGTTGTTGTGCTC+AGG	0.449173	4.1:-39012640	MS.gene08618:CDS
CTTGTAGGTGTTGAGGTTTG+TGG	0.466318	4.1:+39013079	None:intergenic
AAATTCCAATTCCAAAGAAC+AGG	0.466460	4.1:-39012948	MS.gene08618:CDS
TTCTGTTATGGAGTTGTTTG+AGG	0.467984	4.1:+39013126	None:intergenic
TCGAATTAGGACGATGTTGA+AGG	0.492300	4.1:-39012846	MS.gene08618:CDS
ATCGTCCTAATTCGATTCAT+AGG	0.501138	4.1:+39012854	None:intergenic
ATGGTTGTTGTGCTCAGGAT+CGG	0.518192	4.1:-39012635	MS.gene08618:CDS
TTTGGAATTGGAATTTCCGT+TGG	0.525440	4.1:+39012955	None:intergenic
AAACGCAACCACATCGGTGT+TGG	0.526391	4.1:+39012495	None:intergenic
CACCGATAAGCTACTAGAAG+TGG	0.540179	4.1:+39012443	None:intergenic
TCCAAAGAACAGGAAGAAAG+TGG	0.544342	4.1:-39012938	MS.gene08618:CDS
GAAGTTTCTCGAACAATTAG+CGG	0.558467	4.1:-39012667	MS.gene08618:CDS
CGAATTAGGACGATGTTGAA+GGG	0.589179	4.1:-39012845	MS.gene08618:CDS
AAGAAACTCAATTTCACCAA+CGG	0.626812	4.1:-39012971	MS.gene08618:CDS
TTTGGCTATTAACAATGCTG+CGG	0.635948	4.1:-39012786	MS.gene08618:intron
GTTTCTCGAACAATTAGCGG+AGG	0.647450	4.1:-39012664	MS.gene08618:CDS
AAGAAGAAAGAAACATGCAA+AGG	0.664587	4.1:-39012915	MS.gene08618:CDS
TAATTTAAACGCAACCACAT+CGG	0.668137	4.1:+39012489	None:intergenic
GTCAGTGTCCAACACCGATG+TGG	0.705494	4.1:-39012503	MS.gene08618:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTATTGTTATGGTTTTTAT+TGG	-	chr4.1:39012856-39012875	MS.gene08618:CDS	15.0%
!!	GTTGCGTTTAAATTATTATT+CGG	-	chr4.1:39013108-39013127	MS.gene08618:CDS	20.0%
!!!	ATTGTTATGTGTTTTTGATT+TGG	-	chr4.1:39012881-39012900	MS.gene08618:CDS	20.0%
	AAATTCCAATTCCAAAGAAC+AGG	-	chr4.1:39012641-39012660	MS.gene08618:CDS	30.0%
	AAGAAACTCAATTTCACCAA+CGG	-	chr4.1:39012618-39012637	MS.gene08618:CDS	30.0%
	AAGAAGAAAGAAACATGCAA+AGG	-	chr4.1:39012674-39012693	MS.gene08618:intron	30.0%
	TAATTTAAACGCAACCACAT+CGG	+	chr4.1:39013103-39013122	None:intergenic	30.0%
	TGTTTCCTATGAATCGAATT+AGG	-	chr4.1:39012730-39012749	MS.gene08618:intron	30.0%
	TTCGTTCTTTATCTTCTCTT+TGG	+	chr4.1:39012571-39012590	None:intergenic	30.0%
!	TCTTTCTTGATTTTGCTTGT+AGG	+	chr4.1:39012528-39012547	None:intergenic	30.0%
!!!	ATTTGGTGTTTGTGTTTTGT+AGG	-	chr4.1:39012898-39012917	MS.gene08618:CDS	30.0%
!!!	CTTCTTGTTGTTTTCTGTTA+TGG	+	chr4.1:39012478-39012497	None:intergenic	30.0%
	AGCTGTTACATTTGTACTTG+CGG	-	chr4.1:39013040-39013059	MS.gene08618:CDS	35.0%
	ATCGTCCTAATTCGATTCAT+AGG	+	chr4.1:39012738-39012757	None:intergenic	35.0%
	GAAGTTTCTCGAACAATTAG+CGG	-	chr4.1:39012922-39012941	MS.gene08618:CDS	35.0%
	TTCTGTTATGGAGTTGTTTG+AGG	+	chr4.1:39012466-39012485	None:intergenic	35.0%
	TTTGGCTATTAACAATGCTG+CGG	-	chr4.1:39012803-39012822	MS.gene08618:CDS	35.0%
!	TGTTTCTCAGGAAGCTATTT+TGG	-	chr4.1:39012785-39012804	MS.gene08618:intron	35.0%
!	TTTGGAATTGGAATTTCCGT+TGG	+	chr4.1:39012637-39012656	None:intergenic	35.0%
!!	TTCTTCCTGTTCTTTGGAAT+TGG	+	chr4.1:39012649-39012668	None:intergenic	35.0%
	CGAATTAGGACGATGTTGAA+GGG	-	chr4.1:39012744-39012763	MS.gene08618:intron	40.0%
	TCCAAAGAACAGGAAGAAAG+TGG	-	chr4.1:39012651-39012670	MS.gene08618:CDS	40.0%
	TCCACTTTCTTCCTGTTCTT+TGG	+	chr4.1:39012655-39012674	None:intergenic	40.0%
	TCGAATTAGGACGATGTTGA+AGG	-	chr4.1:39012743-39012762	MS.gene08618:intron	40.0%
!	TGTTGAGGTTTGTGGCTTTT+TGG	+	chr4.1:39012505-39012524	None:intergenic	40.0%
!!	GATTTTGCTTGTAGGTGTTG+AGG	+	chr4.1:39012520-39012539	None:intergenic	40.0%
	ATGGTTGTTGTGCTCAGGAT+CGG	-	chr4.1:39012954-39012973	MS.gene08618:CDS	45.0%
	CAATTAGCGGAGGATGCTTA+TGG	-	chr4.1:39012935-39012954	MS.gene08618:CDS	45.0%
	GTTTCTCGAACAATTAGCGG+AGG	-	chr4.1:39012925-39012944	MS.gene08618:CDS	45.0%
	TAGCGATTTGCGTGTTTCTC+AGG	-	chr4.1:39012773-39012792	MS.gene08618:intron	45.0%
	TGCGGCTTGTGTTTAGTGTT+TGG	-	chr4.1:39013058-39013077	MS.gene08618:CDS	45.0%
	TGCTTATGGTTGTTGTGCTC+AGG	-	chr4.1:39012949-39012968	MS.gene08618:CDS	45.0%
!!	CTTGTAGGTGTTGAGGTTTG+TGG	+	chr4.1:39012513-39012532	None:intergenic	45.0%
!!!	TTTTTTTTTTTTTATTGTTA+TGG	-	chr4.1:39012846-39012865	MS.gene08618:CDS	5.0%
	AAACGCAACCACATCGGTGT+TGG	+	chr4.1:39013097-39013116	None:intergenic	50.0%
	GTCAGTGTCCAACACCGATG+TGG	-	chr4.1:39013086-39013105	MS.gene08618:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	39012456	39013155	39012456	ID=MS.gene08618
chr4.1	mRNA	39012456	39013155	39012456	ID=MS.gene08618.t1;Parent=MS.gene08618
chr4.1	exon	39012787	39013155	39012787	ID=MS.gene08618.t1.exon1;Parent=MS.gene08618.t1
chr4.1	CDS	39012787	39013155	39012787	ID=cds.MS.gene08618.t1;Parent=MS.gene08618.t1
chr4.1	exon	39012607	39012691	39012607	ID=MS.gene08618.t1.exon2;Parent=MS.gene08618.t1
chr4.1	CDS	39012607	39012691	39012607	ID=cds.MS.gene08618.t1;Parent=MS.gene08618.t1
chr4.1	exon	39012456	39012520	39012456	ID=MS.gene08618.t1.exon3;Parent=MS.gene08618.t1
chr4.1	CDS	39012456	39012520	39012456	ID=cds.MS.gene08618.t1;Parent=MS.gene08618.t1

Gene Sequence

>MS.gene08618

ATGGCTTCCTCAAACAACTCCATAACAGAAAACAACAAGAAGAAATCCAAAAAGCCACAAACCTCAACACCTACAAGCAAAATCAAGAAAGACAACAAAAGCATTGAAAAATCCAAAGAGAAGATAAAGAACGAAGAGAACAAGAACAACAACAAGAATAAGAAGAAACTCAATTTCACCAACGGAAATTCCAATTCCAAAGAACAGGAAGAAAGTGGAAGAAGAAAGAAACATGCAAAGGACGATGACGAAGAAGAAGATGCGAAAACGCATGTGTTTCCTATGAATCGAATTAGGACGATGTTGAAGGGTGAAATTAGCGATTTGCGTGTTTCTCAGGAAGCTATTTTGGCTATTAACAATGCTGCGGTAAATTTATGAATGTTTATGTTTTTTTTTTTTTATTGTTATGGTTTTTATTGGTTATTGTTATGTGTTTTTGATTTGGTGTTTGTGTTTTGTAGGAGAAGTTTCTCGAACAATTAGCGGAGGATGCTTATGGTTGTTGTGCTCAGGATCGGAAGAAGTCTTTGAGCTACAATCATCTATGTAATGTCTCTTTTGCTCTCTACTTTTTTGCTATAAGCTGTTACATTTGTACTTGCGGCTTGTGTTTAGTGTTTGGTATGTGTCAGTGTCCAACACCGATGTGGTTGCGTTTAAATTATTATTCGGTGTCTATGTGTTAAACCACTTCTAG

Protein sequence

>MS.gene08618.t1

MASSNNSITENNKKKSKKPQTSTPTSKIKKDNKSIEKSKEKIKNEENKNNNKNKKKLNFTNGNSNSKEQEESGRRKKHAKDDDEEEDAKTHVFPMNRIRTMLKGEISDLRVSQEAILAINNAAEKFLEQLAEDAYGCCAQDRKKSLSYNHLLSNTDVVAFKLLFGVYVLNHF