AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20621


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20621.t1 MTR_0856s0010 61.310 168 51 4 54 217 2 159 2.21e-63 197
MS.gene20621.t1 MTR_7g115320 48.485 66 31 1 179 244 6 68 4.56e-14 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20621.t1 AT4G15090 30.890 191 126 3 93 277 50 240 6.73e-16 78.2
MS.gene20621.t1 AT4G15090 30.890 191 126 3 93 277 50 240 6.73e-16 78.2
MS.gene20621.t1 AT4G15090 30.890 191 126 3 93 277 50 240 6.73e-16 78.2
MS.gene20621.t1 AT4G15090 30.890 191 126 3 93 277 50 240 6.73e-16 78.2
MS.gene20621.t1 AT4G15090 30.890 191 126 3 93 277 50 240 6.73e-16 78.2
MS.gene20621.t1 AT1G76320 26.984 189 129 4 98 284 1 182 5.04e-11 63.5
MS.gene20621.t1 AT1G76320 26.984 189 129 4 98 284 1 182 5.38e-11 63.5
MS.gene20621.t1 AT1G76320 26.984 189 129 4 98 284 1 182 5.38e-11 63.5
MS.gene20621.t1 AT4G38180 25.510 196 136 5 93 279 72 266 7.05e-11 63.2
MS.gene20621.t1 AT1G76320 26.984 189 129 4 98 284 1 182 7.18e-11 63.2

Find 41 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGATAGTTCAAAGAATT+TGG 0.188161 6.3:+4142680 MS.gene20621:CDS
CCCACATCTCGTTTGCAAAA+TGG 0.190562 6.3:-4143344 None:intergenic
TGGAGAGTATGCTTTGAAAA+AGG 0.195927 6.3:+4142956 MS.gene20621:CDS
TTGAACAATCTTCCCTTTGC+AGG 0.266353 6.3:-4143200 None:intergenic
CACCCTCTATTGGAACTTCA+TGG 0.318363 6.3:+4142662 MS.gene20621:CDS
CTCCTGGATCATCAGTGCTT+TGG 0.328920 6.3:-4143262 None:intergenic
TTGGCACTTTCACCCTCTAT+TGG 0.334745 6.3:+4142652 MS.gene20621:CDS
GCACGTTGGAAGCCTGAAAC+AGG 0.364470 6.3:+4142894 MS.gene20621:CDS
CAACCTCTTGTTAAAGATGT+AGG 0.366945 6.3:+4143305 MS.gene20621:CDS
ACATCAAGAAAAGGAGATAA+TGG 0.382507 6.3:+4142999 MS.gene20621:CDS
TTCCAACTTAATGCAAATCT+TGG 0.384721 6.3:-4143120 None:intergenic
GAAGATTGTTCAAGGCTAAC+AGG 0.389692 6.3:+4143210 MS.gene20621:CDS
TACAAGGAACGACATGCAAT+TGG 0.415503 6.3:+4143377 MS.gene20621:CDS
AACTTAATGCAAATCTTGGC+TGG 0.446598 6.3:-4143116 None:intergenic
AGCCAAGATTTGCATTAAGT+TGG 0.449507 6.3:+4143118 MS.gene20621:CDS
GTCTTCTTGATCCTCACTCC+TGG 0.453631 6.3:-4143278 None:intergenic
TATCCATGAAGTTCCAATAG+AGG 0.493111 6.3:-4142665 None:intergenic
CAGGGCTAGTCTCGTGAGAA+TGG 0.497072 6.3:-4143181 None:intergenic
AGTTGGAAGACGATGGTTTG+TGG 0.499523 6.3:+4143135 MS.gene20621:CDS
TCAAAGAATTTGGTAGATTG+TGG 0.500305 6.3:+4142690 MS.gene20621:CDS
AAGATTAGAACATCAAGAAA+AGG 0.501587 6.3:+4142990 MS.gene20621:CDS
CGACATGCAATTGGAAAAGA+AGG 0.505598 6.3:+4143386 MS.gene20621:CDS
TGCAAAGGGAAGATTGTTCA+AGG 0.507962 6.3:+4143202 MS.gene20621:CDS
GATAACTCTAATGATAATGA+AGG 0.530992 6.3:+4142774 MS.gene20621:CDS
GATAACTCTAATGATAATGA+AGG 0.530992 6.3:+4142807 MS.gene20621:CDS
TGAACAATCTTCCCTTTGCA+GGG 0.541033 6.3:-4143199 None:intergenic
ATCCAAAGCACTGATGATCC+AGG 0.543376 6.3:+4143260 MS.gene20621:CDS
TGTGGGAAATTATGTTTACA+AGG 0.554375 6.3:+4143361 MS.gene20621:CDS
TGTGGTACTATTCATGATGA+AGG 0.556353 6.3:+4142708 MS.gene20621:CDS
CACGAGACTAGCCCTGCAAA+GGG 0.557494 6.3:+4143188 MS.gene20621:CDS
TCACGAGACTAGCCCTGCAA+AGG 0.558154 6.3:+4143187 MS.gene20621:CDS
GATAACTCGAATGATAATGA+AGG 0.559715 6.3:+4142741 MS.gene20621:CDS
ATCCATGAAGTTCCAATAGA+GGG 0.567931 6.3:-4142664 None:intergenic
AATTCTTGCTTGCTCAAGAG+AGG 0.580330 6.3:+4143037 MS.gene20621:CDS
TGCATTAAGTTGGAAGACGA+TGG 0.588214 6.3:+4143128 MS.gene20621:CDS
ATTCTTGCTTGCTCAAGAGA+GGG 0.604718 6.3:+4143038 MS.gene20621:CDS
GCTCCTACATCTTTAACAAG+AGG 0.607465 6.3:-4143308 None:intergenic
GCAATTGGAAAAGAAGGTGA+TGG 0.609009 6.3:+4143392 MS.gene20621:CDS
CACGTTGGAAGCCTGAAACA+GGG 0.628231 6.3:+4142895 MS.gene20621:CDS
GCACTGATGATCCAGGAGTG+AGG 0.654669 6.3:+4143267 MS.gene20621:CDS
CTAATGATGATGAAGCACGT+TGG 0.674855 6.3:+4142880 MS.gene20621:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGCTATTTTTGTAAAATGA+GGG + chr6.3:4143427-4143446 MS.gene20621:CDS 20.0%
!!! GATGATGTTAAAACATTTTA+TGG + chr6.3:4142936-4142955 MS.gene20621:CDS 20.0%
!!! TAAGCTATTTTTGTAAAATG+AGG + chr6.3:4143426-4143445 MS.gene20621:CDS 20.0%
! AAGATTAGAACATCAAGAAA+AGG + chr6.3:4142990-4143009 MS.gene20621:CDS 25.0%
!! GATAACTCTAATGATAATGA+AGG + chr6.3:4142774-4142793 MS.gene20621:CDS 25.0%
!!! GACAATTTTTTTCCTTAGAT+GGG - chr6.3:4143097-4143116 None:intergenic 25.0%
ACATCAAGAAAAGGAGATAA+TGG + chr6.3:4142999-4143018 MS.gene20621:CDS 30.0%
CATGGATAGTTCAAAGAATT+TGG + chr6.3:4142680-4142699 MS.gene20621:CDS 30.0%
TCAAAGAATTTGGTAGATTG+TGG + chr6.3:4142690-4142709 MS.gene20621:CDS 30.0%
TGTGGGAAATTATGTTTACA+AGG + chr6.3:4143361-4143380 MS.gene20621:CDS 30.0%
TTCCAACTTAATGCAAATCT+TGG - chr6.3:4143123-4143142 None:intergenic 30.0%
! GATAACTCGAATGATAATGA+AGG + chr6.3:4142741-4142760 MS.gene20621:CDS 30.0%
!!! ATGCTTTGAAAAAGGGTTTT+AGG + chr6.3:4142964-4142983 MS.gene20621:CDS 30.0%
!!! GGACAATTTTTTTCCTTAGA+TGG - chr6.3:4143098-4143117 None:intergenic 30.0%
!!! TGACTTATTTTGTGCAGTTT+TGG + chr6.3:4142628-4142647 MS.gene20621:CDS 30.0%
AACTTAATGCAAATCTTGGC+TGG - chr6.3:4143119-4143138 None:intergenic 35.0%
AGCCAAGATTTGCATTAAGT+TGG + chr6.3:4143118-4143137 MS.gene20621:CDS 35.0%
ATCCATGAAGTTCCAATAGA+GGG - chr6.3:4142667-4142686 None:intergenic 35.0%
CAACCTCTTGTTAAAGATGT+AGG + chr6.3:4143305-4143324 MS.gene20621:CDS 35.0%
GTTAAAAACTCTCCCATCTA+AGG + chr6.3:4143082-4143101 MS.gene20621:CDS 35.0%
TATCCATGAAGTTCCAATAG+AGG - chr6.3:4142668-4142687 None:intergenic 35.0%
TGTGGTACTATTCATGATGA+AGG + chr6.3:4142708-4142727 MS.gene20621:CDS 35.0%
!! GGAGAGTATGCTTTGAAAAA+GGG + chr6.3:4142957-4142976 MS.gene20621:CDS 35.0%
!! TGGAGAGTATGCTTTGAAAA+AGG + chr6.3:4142956-4142975 MS.gene20621:CDS 35.0%
!!! CTTTGAAAAAGGGTTTTAGG+TGG + chr6.3:4142967-4142986 MS.gene20621:CDS 35.0%
!!! TATTTTGTGCAGTTTTGGCT+TGG + chr6.3:4142633-4142652 MS.gene20621:CDS 35.0%
AATTCTTGCTTGCTCAAGAG+AGG + chr6.3:4143037-4143056 MS.gene20621:CDS 40.0%
ATTCTTGCTTGCTCAAGAGA+GGG + chr6.3:4143038-4143057 MS.gene20621:CDS 40.0%
CGACATGCAATTGGAAAAGA+AGG + chr6.3:4143386-4143405 MS.gene20621:CDS 40.0%
GAAAAACACATCCCTGTTTC+AGG - chr6.3:4142909-4142928 None:intergenic 40.0%
GAAGATTGTTCAAGGCTAAC+AGG + chr6.3:4143210-4143229 MS.gene20621:CDS 40.0%
GCAATTGGAAAAGAAGGTGA+TGG + chr6.3:4143392-4143411 MS.gene20621:CDS 40.0%
GCTCCTACATCTTTAACAAG+AGG - chr6.3:4143311-4143330 None:intergenic 40.0%
TACAAGGAACGACATGCAAT+TGG + chr6.3:4143377-4143396 MS.gene20621:CDS 40.0%
TGAACAATCTTCCCTTTGCA+GGG - chr6.3:4143202-4143221 None:intergenic 40.0%
TGCAAAGGGAAGATTGTTCA+AGG + chr6.3:4143202-4143221 MS.gene20621:CDS 40.0%
TTGAACAATCTTCCCTTTGC+AGG - chr6.3:4143203-4143222 None:intergenic 40.0%
! ACCATTTTGCAAACGAGATG+TGG + chr6.3:4143343-4143362 MS.gene20621:CDS 40.0%
! CCATTTTGCAAACGAGATGT+GGG + chr6.3:4143344-4143363 MS.gene20621:CDS 40.0%
! CTAATGATGATGAAGCACGT+TGG + chr6.3:4142880-4142899 MS.gene20621:CDS 40.0%
!! TGCATTAAGTTGGAAGACGA+TGG + chr6.3:4143128-4143147 MS.gene20621:CDS 40.0%
AGTTGGAAGACGATGGTTTG+TGG + chr6.3:4143135-4143154 MS.gene20621:CDS 45.0%
CACCCTCTATTGGAACTTCA+TGG + chr6.3:4142662-4142681 MS.gene20621:CDS 45.0%
CCCACATCTCGTTTGCAAAA+TGG - chr6.3:4143347-4143366 None:intergenic 45.0%
TTGGCACTTTCACCCTCTAT+TGG + chr6.3:4142652-4142671 MS.gene20621:CDS 45.0%
!! ATCCAAAGCACTGATGATCC+AGG + chr6.3:4143260-4143279 MS.gene20621:CDS 45.0%
CACGTTGGAAGCCTGAAACA+GGG + chr6.3:4142895-4142914 MS.gene20621:CDS 50.0%
CTCCTGGATCATCAGTGCTT+TGG - chr6.3:4143265-4143284 None:intergenic 50.0%
GTCTTCTTGATCCTCACTCC+TGG - chr6.3:4143281-4143300 None:intergenic 50.0%
GCACGTTGGAAGCCTGAAAC+AGG + chr6.3:4142894-4142913 MS.gene20621:CDS 55.0%
! CACGAGACTAGCCCTGCAAA+GGG + chr6.3:4143188-4143207 MS.gene20621:CDS 55.0%
! GCACTGATGATCCAGGAGTG+AGG + chr6.3:4143267-4143286 MS.gene20621:CDS 55.0%
! TCACGAGACTAGCCCTGCAA+AGG + chr6.3:4143187-4143206 MS.gene20621:CDS 55.0%
!! CAGGGCTAGTCTCGTGAGAA+TGG - chr6.3:4143184-4143203 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 4142627 4143499 4142627 ID=MS.gene20621
chr6.3 mRNA 4142627 4143499 4142627 ID=MS.gene20621.t1;Parent=MS.gene20621
chr6.3 exon 4142627 4143499 4142627 ID=MS.gene20621.t1.exon1;Parent=MS.gene20621.t1
chr6.3 CDS 4142627 4143499 4142627 ID=cds.MS.gene20621.t1;Parent=MS.gene20621.t1
Gene Sequence

>MS.gene20621

ATGACTTATTTTGTGCAGTTTTGGCTTGGCACTTTCACCCTCTATTGGAACTTCATGGATAGTTCAAAGAATTTGGTAGATTGTGGTACTATTCATGATGAAGGTATAGAAGTTGATAACTCGAATGATAATGAAGGTATAGAAGTTGATAACTCTAATGATAATGAAGGTATAGAAGTTGATAACTCTAATGATAATGAAGGTATAGAAGTTGATAACTCTAATGAGAATGAAGTTATAGAAGTTGATAACTCTAATGATGATGAAGCACGTTGGAAGCCTGAAACAGGGATGTGTTTTTCTTGCATAGATGATGTTAAAACATTTTATGGAGAGTATGCTTTGAAAAAGGGTTTTAGGTGGAAGATTAGAACATCAAGAAAAGGAGATAATGGAGAGATCTGCTATGTAATTCTTGCTTGCTCAAGAGAGGGATCTAGATTGTCAAAAAGTTCGTTAAAAACTCTCCCATCTAAGGAAAAAAATTGTCCAGCCAAGATTTGCATTAAGTTGGAAGACGATGGTTTGTGGTACATTAAAAGATTTGAATCTAGCCATTCTCACGAGACTAGCCCTGCAAAGGGAAGATTGTTCAAGGCTAACAGGAAAATGAACTTGCGTGTAAAGAGAACAATCCAAAGCACTGATGATCCAGGAGTGAGGATCAAGAAGACTTTTCAACCTCTTGTTAAAGATGTAGGAGCGCATGAAATTACACCATTTTGCAAACGAGATGTGGGAAATTATGTTTACAAGGAACGACATGCAATTGGAAAAGAAGGTGATGGCATCGTCTTGATAAGCTATTTTTGTAAAATGAGGGTGCAAAATCAAAATCAAAATGCTTTCATCTTATCTATGTTAGTTTCTTAG

Protein sequence

>MS.gene20621.t1

MTYFVQFWLGTFTLYWNFMDSSKNLVDCGTIHDEGIEVDNSNDNEGIEVDNSNDNEGIEVDNSNDNEGIEVDNSNENEVIEVDNSNDDEARWKPETGMCFSCIDDVKTFYGEYALKKGFRWKIRTSRKGDNGEICYVILACSREGSRLSKSSLKTLPSKEKNCPAKICIKLEDDGLWYIKRFESSHSHETSPAKGRLFKANRKMNLRVKRTIQSTDDPGVRIKKTFQPLVKDVGAHEITPFCKRDVGNYVYKERHAIGKEGDGIVLISYFCKMRVQNQNQNAFILSMLVS