AlfalfaGEDB | MS.gene24740

M. sativa cultivar XinJiangDaYe / MS.gene24740

KEGG: K00621
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF00583.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24740.t1	XP_003625822.1	98	153	3	0	1	153	1	153	9.30E-79	302.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24740.t1	Q9LFU9	71.9	146	41	0	7	152	3	148	5.0e-57	221.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24740.t1	G7KQU8	98.0	153	3	0	1	153	1	153	6.7e-79	302.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene24740.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene24740	0.852907	3.39E-61	-1.69E-46
MS.gene049159	MS.gene24740	0.843559	1.30E-58	-1.69E-46
MS.gene049435	MS.gene24740	0.809756	1.62E-50	-1.69E-46
MS.gene049501	MS.gene24740	0.805303	1.42E-49	-1.69E-46
MS.gene049756	MS.gene24740	0.805606	1.23E-49	-1.69E-46
MS.gene04984	MS.gene24740	0.806624	7.51E-50	-1.69E-46
MS.gene049850	MS.gene24740	0.802184	6.33E-49	-1.69E-46
MS.gene050317	MS.gene24740	0.840531	8.22E-58	-1.69E-46
MS.gene050442	MS.gene24740	0.823968	1.04E-53	-1.69E-46
MS.gene050689	MS.gene24740	0.800244	1.58E-48	-1.69E-46
MS.gene050719	MS.gene24740	0.81558	8.59E-52	-1.69E-46
MS.gene050721	MS.gene24740	0.816877	4.41E-52	-1.69E-46
MS.gene050724	MS.gene24740	0.806083	9.77E-50	-1.69E-46
MS.gene050807	MS.gene24740	0.802375	5.78E-49	-1.69E-46
MS.gene050815	MS.gene24740	0.818573	1.83E-52	-1.69E-46
MS.gene051102	MS.gene24740	0.81881	1.61E-52	-1.69E-46
MS.gene051180	MS.gene24740	0.811002	8.70E-51	-1.69E-46
MS.gene051198	MS.gene24740	0.805584	1.24E-49	-1.69E-46
MS.gene051210	MS.gene24740	0.816388	5.67E-52	-1.69E-46
MS.gene051243	MS.gene24740	0.800242	1.58E-48	-1.69E-46
MS.gene051247	MS.gene24740	0.819247	1.28E-52	-1.69E-46
MS.gene051328	MS.gene24740	0.801437	9.01E-49	-1.69E-46
MS.gene051490	MS.gene24740	0.8056	1.23E-49	-1.69E-46
MS.gene051744	MS.gene24740	0.810232	1.28E-50	-1.69E-46
MS.gene051757	MS.gene24740	0.80469	1.91E-49	-1.69E-46
MS.gene051975	MS.gene24740	0.802744	4.85E-49	-1.69E-46
MS.gene052012	MS.gene24740	0.850387	1.76E-60	-1.69E-46
MS.gene052020	MS.gene24740	0.805724	1.16E-49	-1.69E-46
MS.gene052384	MS.gene24740	0.818682	1.73E-52	-1.69E-46
MS.gene052516	MS.gene24740	0.826783	2.24E-54	-1.69E-46
MS.gene052611	MS.gene24740	0.812048	5.16E-51	-1.69E-46
MS.gene052665	MS.gene24740	0.813605	2.35E-51	-1.69E-46
MS.gene052773	MS.gene24740	0.807743	4.35E-50	-1.69E-46
MS.gene052863	MS.gene24740	0.823818	1.13E-53	-1.69E-46
MS.gene052896	MS.gene24740	0.831134	1.98E-55	-1.69E-46
MS.gene053008	MS.gene24740	0.817189	3.75E-52	-1.69E-46
MS.gene053226	MS.gene24740	0.827392	1.60E-54	-1.69E-46
MS.gene054236	MS.gene24740	0.819299	1.25E-52	-1.69E-46
MS.gene054472	MS.gene24740	0.839993	1.14E-57	-1.69E-46
MS.gene05458	MS.gene24740	0.81713	3.87E-52	-1.69E-46
MS.gene05462	MS.gene24740	0.816574	5.16E-52	-1.69E-46
MS.gene054651	MS.gene24740	0.810646	1.04E-50	-1.69E-46
MS.gene05517	MS.gene24740	0.820766	5.77E-53	-1.69E-46
MS.gene055313	MS.gene24740	0.801758	7.74E-49	-1.69E-46
MS.gene055465	MS.gene24740	0.808152	3.56E-50	-1.69E-46
MS.gene055466	MS.gene24740	0.833952	3.97E-56	-1.69E-46
MS.gene055468	MS.gene24740	0.817931	2.55E-52	-1.69E-46
MS.gene055512	MS.gene24740	0.806269	8.93E-50	-1.69E-46
MS.gene055623	MS.gene24740	0.850122	2.08E-60	-1.69E-46
MS.gene055818	MS.gene24740	0.815128	1.08E-51	-1.69E-46
MS.gene055926	MS.gene24740	0.824564	7.54E-54	-1.69E-46
MS.gene05604	MS.gene24740	0.815268	1.01E-51	-1.69E-46
MS.gene056308	MS.gene24740	0.811355	7.30E-51	-1.69E-46
MS.gene056760	MS.gene24740	0.819963	8.81E-53	-1.69E-46
MS.gene057034	MS.gene24740	0.800231	1.59E-48	-1.69E-46
MS.gene057360	MS.gene24740	0.811582	6.52E-51	-1.69E-46
MS.gene057693	MS.gene24740	0.818241	2.17E-52	-1.69E-46
MS.gene057869	MS.gene24740	0.820592	6.32E-53	-1.69E-46
MS.gene057915	MS.gene24740	0.840496	8.40E-58	-1.69E-46
MS.gene058010	MS.gene24740	0.813228	2.85E-51	-1.69E-46
MS.gene058084	MS.gene24740	0.807062	6.07E-50	-1.69E-46
MS.gene058146	MS.gene24740	0.806913	6.53E-50	-1.69E-46
MS.gene058625	MS.gene24740	0.810906	9.13E-51	-1.69E-46
MS.gene058906	MS.gene24740	0.807328	5.33E-50	-1.69E-46
MS.gene058979	MS.gene24740	0.805137	1.54E-49	-1.69E-46
MS.gene059713	MS.gene24740	0.803598	3.23E-49	-1.69E-46
MS.gene059725	MS.gene24740	0.818795	1.63E-52	-1.69E-46
MS.gene059842	MS.gene24740	0.843904	1.05E-58	-1.69E-46
MS.gene060322	MS.gene24740	0.815409	9.38E-52	-1.69E-46
MS.gene06052	MS.gene24740	0.807136	5.86E-50	-1.69E-46
MS.gene060587	MS.gene24740	0.815313	9.85E-52	-1.69E-46
MS.gene060637	MS.gene24740	0.83952	1.51E-57	-1.69E-46
MS.gene060683	MS.gene24740	0.817697	2.88E-52	-1.69E-46
MS.gene060685	MS.gene24740	0.819622	1.05E-52	-1.69E-46
MS.gene060802	MS.gene24740	0.813198	2.89E-51	-1.69E-46
MS.gene060803	MS.gene24740	0.852008	6.12E-61	-1.69E-46
MS.gene060998	MS.gene24740	0.823813	1.13E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24740.t1	MTR_7g104610	98.039	153	3	0	1	153	1	153	1.93e-107	303

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24740.t1	AT5G15770	71.918	146	41	0	7	152	3	148	3.14e-74	219

Find 27 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCCGTTGCAATAATCTTTC+CGG	0.199227	7.3:-9886035	None:intergenic
GTCTGTAATCTCTAATCTTC+TGG	0.252175	7.3:-9885873	None:intergenic
TCGTTCTGTTGGGTGTTATA+AGG	0.300594	7.3:+9886189	MS.gene24740:CDS
TAAGAAATTGTGGTAAAGTT+GGG	0.348212	7.3:+9886083	MS.gene24740:CDS
TTAAGAAATTGTGGTAAAGT+TGG	0.366943	7.3:+9886082	MS.gene24740:CDS
ACCGATCATGCTCGTTCTGT+TGG	0.371791	7.3:+9886178	MS.gene24740:CDS
AGTGCTCGTGGGAAACAATT+GGG	0.376890	7.3:+9886133	MS.gene24740:CDS
ACCGGAAAGATTATTGCAAC+GGG	0.406061	7.3:+9886034	MS.gene24740:CDS
GTTATTGAAGATGAGATTAC+CGG	0.417687	7.3:+9886016	MS.gene24740:CDS
AAGTGCTCGTGGGAAACAAT+TGG	0.427736	7.3:+9886132	MS.gene24740:CDS
CCGATCATGCTCGTTCTGTT+GGG	0.443895	7.3:+9886179	MS.gene24740:CDS
AGTCTCTGATAAGGAATTTG+AGG	0.451854	7.3:+9885946	MS.gene24740:CDS
TTGTGATTCAGTCTCTGATA+AGG	0.468883	7.3:+9885937	MS.gene24740:CDS
TTTCGAGAGGTTGATTCACT+TGG	0.471380	7.3:+9885974	MS.gene24740:CDS
CGATTGCAGTGTTGAGAACA+AGG	0.494534	7.3:+9886219	MS.gene24740:CDS
CTATGAGAAATGTGGTTTCA+AGG	0.496011	7.3:+9886246	MS.gene24740:CDS
CCCAACAGAACGAGCATGAT+CGG	0.532044	7.3:-9886179	None:intergenic
TACCGGAAAGATTATTGCAA+CGG	0.546187	7.3:+9886033	MS.gene24740:CDS
CAAGGAGAAAGAAGTTCAGA+TGG	0.566779	7.3:+9886264	MS.gene24740:CDS
GTTGTGGATTCAAGTGCTCG+TGG	0.610212	7.3:+9886121	MS.gene24740:CDS
ATTTGAGGATCGATTTCGAG+AGG	0.616403	7.3:+9885961	MS.gene24740:CDS
GCATATAGAAGATGTTGTTG+TGG	0.621571	7.3:+9886105	MS.gene24740:CDS
GTGCTCGTGGGAAACAATTG+GGG	0.624260	7.3:+9886134	MS.gene24740:CDS
TTGTGGATTCAAGTGCTCGT+GGG	0.642750	7.3:+9886122	MS.gene24740:CDS
AAGGTGTTCTATGAGAAATG+TGG	0.671486	7.3:+9886238	MS.gene24740:CDS
TTAGAAGATGCAAAACAGTG+AGG	0.719583	7.3:+9885835	None:intergenic
CCGGAAAGATTATTGCAACG+GGG	0.727047	7.3:+9886035	MS.gene24740:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAGAAATTTTTAAGAAATTG+TGG	+	chr7.3:9886073-9886092	MS.gene24740:CDS	15.0%
!	TAAGAAATTGTGGTAAAGTT+GGG	+	chr7.3:9886083-9886102	MS.gene24740:CDS	25.0%
!	TTAAGAAATTGTGGTAAAGT+TGG	+	chr7.3:9886082-9886101	MS.gene24740:CDS	25.0%
	GAGATTACAGACAAGAAAAA+AGG	+	chr7.3:9885884-9885903	MS.gene24740:CDS	30.0%
	GTTATTGAAGATGAGATTAC+CGG	+	chr7.3:9886016-9886035	MS.gene24740:CDS	30.0%
	AGTCTCTGATAAGGAATTTG+AGG	+	chr7.3:9885946-9885965	MS.gene24740:CDS	35.0%
	CTATGAGAAATGTGGTTTCA+AGG	+	chr7.3:9886246-9886265	MS.gene24740:CDS	35.0%
	GCATATAGAAGATGTTGTTG+TGG	+	chr7.3:9886105-9886124	MS.gene24740:CDS	35.0%
	GTCTGTAATCTCTAATCTTC+TGG	-	chr7.3:9885876-9885895	None:intergenic	35.0%
	TACCGGAAAGATTATTGCAA+CGG	+	chr7.3:9886033-9886052	MS.gene24740:CDS	35.0%
	TTGTGATTCAGTCTCTGATA+AGG	+	chr7.3:9885937-9885956	MS.gene24740:CDS	35.0%
!	AAGGTGTTCTATGAGAAATG+TGG	+	chr7.3:9886238-9886257	MS.gene24740:CDS	35.0%
	ACCGGAAAGATTATTGCAAC+GGG	+	chr7.3:9886034-9886053	MS.gene24740:CDS	40.0%
	CAAGGAGAAAGAAGTTCAGA+TGG	+	chr7.3:9886264-9886283	MS.gene24740:CDS	40.0%
!	ATTTGAGGATCGATTTCGAG+AGG	+	chr7.3:9885961-9885980	MS.gene24740:CDS	40.0%
!	TCGTTCTGTTGGGTGTTATA+AGG	+	chr7.3:9886189-9886208	MS.gene24740:CDS	40.0%
!	TTTCGAGAGGTTGATTCACT+TGG	+	chr7.3:9885974-9885993	MS.gene24740:CDS	40.0%
	AAGTGCTCGTGGGAAACAAT+TGG	+	chr7.3:9886132-9886151	MS.gene24740:CDS	45.0%
	AGTGCTCGTGGGAAACAATT+GGG	+	chr7.3:9886133-9886152	MS.gene24740:CDS	45.0%
	CCCCGTTGCAATAATCTTTC+CGG	-	chr7.3:9886038-9886057	None:intergenic	45.0%
	CCGGAAAGATTATTGCAACG+GGG	+	chr7.3:9886035-9886054	MS.gene24740:CDS	45.0%
	TTGTGGATTCAAGTGCTCGT+GGG	+	chr7.3:9886122-9886141	MS.gene24740:CDS	45.0%
!	CGATTGCAGTGTTGAGAACA+AGG	+	chr7.3:9886219-9886238	MS.gene24740:CDS	45.0%
	ACCGATCATGCTCGTTCTGT+TGG	+	chr7.3:9886178-9886197	MS.gene24740:CDS	50.0%
	CCCAACAGAACGAGCATGAT+CGG	-	chr7.3:9886182-9886201	None:intergenic	50.0%
	CCGATCATGCTCGTTCTGTT+GGG	+	chr7.3:9886179-9886198	MS.gene24740:CDS	50.0%
	GTGCTCGTGGGAAACAATTG+GGG	+	chr7.3:9886134-9886153	MS.gene24740:CDS	50.0%
	GTTGTGGATTCAAGTGCTCG+TGG	+	chr7.3:9886121-9886140	MS.gene24740:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	9885842	9886303	9885842	ID=MS.gene24740
chr7.3	mRNA	9885842	9886303	9885842	ID=MS.gene24740.t1;Parent=MS.gene24740
chr7.3	exon	9885842	9886303	9885842	ID=MS.gene24740.t1.exon1;Parent=MS.gene24740.t1
chr7.3	CDS	9885842	9886303	9885842	ID=cds.MS.gene24740.t1;Parent=MS.gene24740.t1

Gene Sequence

>MS.gene24740

ATGCAAAACAGTGAGGAACAAAAGTATAGAGCCAGAAGATTAGAGATTACAGACAAGAAAAAAGGTTTCATAGAATTACTACAACAGCTTAGCGTTTGTGATTCAGTCTCTGATAAGGAATTTGAGGATCGATTTCGAGAGGTTGATTCACTTGGAGATGATCATGTTATATGTGTTATTGAAGATGAGATTACCGGAAAGATTATTGCAACGGGGAGTGTGTTTATTGAGAAGAAATTTTTAAGAAATTGTGGTAAAGTTGGGCATATAGAAGATGTTGTTGTGGATTCAAGTGCTCGTGGGAAACAATTGGGGAAGAAAGTAATTAACTTTCTTACCGATCATGCTCGTTCTGTTGGGTGTTATAAGGTAATTCTCGATTGCAGTGTTGAGAACAAGGTGTTCTATGAGAAATGTGGTTTCAAGGAGAAAGAAGTTCAGATGGCTATGTATTTTGTTTGA

Protein sequence

>MS.gene24740.t1

MQNSEEQKYRARRLEITDKKKGFIELLQQLSVCDSVSDKEFEDRFREVDSLGDDHVICVIEDEITGKIIATGSVFIEKKFLRNCGKVGHIEDVVVDSSARGKQLGKKVINFLTDHARSVGCYKVILDCSVENKVFYEKCGFKEKEVQMAMYFV