AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25829


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25829.t1 MTR_3g079820 92.188 128 9 1 45 171 1 128 7.83e-82 238
MS.gene25829.t1 MTR_3g079783 75.962 104 20 4 71 171 195 296 1.39e-41 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25829.t1 AT5G20935 79.012 81 17 0 89 169 37 117 5.41e-41 134
MS.gene25829.t1 AT5G20935 77.083 48 11 0 89 136 37 84 3.65e-19 78.2

Find 38 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAATTTACCTTGCATTT+TGG 0.168617 3.2:+70160295 None:intergenic
ACTAATGACACTGAAATATT+TGG 0.193807 3.2:+70159485 None:intergenic
CCTTGCCTCAGAGTTAATTC+TGG 0.251411 3.2:-70159338 MS.gene25829:CDS
GATTTGTGGTTTCAATGTTT+TGG 0.261828 3.2:+70159615 None:intergenic
ACTATACTCAACCAGATCTA+AGG 0.289522 3.2:+70158814 None:intergenic
TGGAGCTTCGATGATAATAA+TGG 0.291436 3.2:+70159388 None:intergenic
AAACGTTCAAAGTTGGATAA+AGG 0.314200 3.2:-70159416 MS.gene25829:CDS
CCGTACTGCCCACACATCTT+TGG 0.332641 3.2:+70158880 None:intergenic
CGTACTGCCCACACATCTTT+GGG 0.353292 3.2:+70158881 None:intergenic
ATTGGAACTTATCTTATTGA+TGG 0.358224 3.2:-70158851 MS.gene25829:CDS
GTTGCAATGTTTCTGATTTG+TGG 0.375721 3.2:+70159601 None:intergenic
GGAGCTTCGATGATAATAAT+GGG 0.430762 3.2:+70159389 None:intergenic
AACGTTCAAAGTTGGATAAA+GGG 0.431347 3.2:-70159415 MS.gene25829:CDS
ATATTTCAAGCCCTTAGATC+TGG 0.435238 3.2:-70158825 MS.gene25829:CDS
ATATTTCAGTGTCATTAGTA+AGG 0.457947 3.2:-70159481 MS.gene25829:CDS
CTGTTGTTGAACTTTAGAAA+TGG 0.461418 3.2:+70159530 None:intergenic
ATGTCTTTGTCCCTTTCTTG+TGG 0.510900 3.2:-70159563 MS.gene25829:CDS
CTTTGTGATACCACAAGAAA+GGG 0.530774 3.2:+70159553 None:intergenic
GCTTTGTGATACCACAAGAA+AGG 0.545899 3.2:+70159552 None:intergenic
CCAAAGATGTGTGGGCAGTA+CGG 0.550493 3.2:-70158880 MS.gene25829:CDS
AATTCTGGCTTAGTCAAACC+TGG 0.553371 3.2:-70159323 MS.gene25829:CDS
CAGAATTAACTCTGAGGCAA+GGG 0.557005 3.2:+70159339 None:intergenic
CTATACTCAACCAGATCTAA+GGG 0.566149 3.2:+70158815 None:intergenic
TGGGCAGTACGGCTCAACAT+TGG 0.579445 3.2:-70158869 MS.gene25829:CDS
TCAAACCTGGTGATGTTGGA+AGG 0.583959 3.2:-70159310 MS.gene25829:intron
GGAAGCCTAACAAAAGATGT+TGG 0.589565 3.2:-70160322 MS.gene25829:CDS
AAGAAAGCCCAAAGATGTGT+GGG 0.590031 3.2:-70158888 MS.gene25829:CDS
CCAGAATTAACTCTGAGGCA+AGG 0.590927 3.2:+70159338 None:intergenic
CAAGAAAGCCCAAAGATGTG+TGG 0.598572 3.2:-70158889 MS.gene25829:CDS
TTAGTCAAACCTGGTGATGT+TGG 0.604419 3.2:-70159314 MS.gene25829:CDS
AAATACCTTCCAACATCACC+AGG 0.606725 3.2:+70159305 None:intergenic
TGTTGGTATACCAAAATGCA+AGG 0.609468 3.2:-70160305 MS.gene25829:intron
AGTCATTCAGTTAATACACA+AGG 0.644330 3.2:-70160344 MS.gene25829:CDS
GTCATTCAGTTAATACACAA+GGG 0.671716 3.2:-70160343 MS.gene25829:CDS
TGAATCAAAAGAGTCATCAG+AGG 0.675313 3.2:-70159456 MS.gene25829:CDS
CTAAGCCAGAATTAACTCTG+AGG 0.700737 3.2:+70159333 None:intergenic
ACAGCTTGACGTATCAACAA+CGG 0.724379 3.2:-70159511 MS.gene25829:CDS
GCTTGACGTATCAACAACGG+AGG 0.741416 3.2:-70159508 MS.gene25829:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTATAAATGATTTAATT+TGG - chr3.2:70160050-70160069 MS.gene25829:intron 10.0%
!! AATTAATATCATGTATAAAA+TGG - chr3.2:70160203-70160222 MS.gene25829:intron 10.0%
!! GTAAATAATTATATATCACT+TGG - chr3.2:70160233-70160252 MS.gene25829:intron 15.0%
!!! AAACTTTTCTATTTTTTTTG+AGG - chr3.2:70159273-70159292 MS.gene25829:intron 15.0%
!!! ATTTTTGTTTCGTTTTTTAT+TGG + chr3.2:70159404-70159423 None:intergenic 15.0%
!! AATGAAAAACTTAAAAGATG+AGG + chr3.2:70159881-70159900 None:intergenic 20.0%
!! GATTAGATACATAGTAAAAA+AGG + chr3.2:70160081-70160100 None:intergenic 20.0%
!!! TGATTATAATTTTTTTGGGA+AGG + chr3.2:70159174-70159193 None:intergenic 20.0%
! AAAATGCAAGGTAAATTTAC+AGG - chr3.2:70158865-70158884 MS.gene25829:CDS 25.0%
! ACTAATGACACTGAAATATT+TGG + chr3.2:70159676-70159695 None:intergenic 25.0%
! ATAGAAACTATGAGTTAAAC+CGG - chr3.2:70159312-70159331 MS.gene25829:CDS 25.0%
! ATTGGAACTTATCTTATTGA+TGG - chr3.2:70160307-70160326 MS.gene25829:CDS 25.0%
! TATCATGTATAAAATGGCAA+TGG - chr3.2:70160209-70160228 MS.gene25829:intron 25.0%
!! ATATTTCAGTGTCATTAGTA+AGG - chr3.2:70159677-70159696 MS.gene25829:intron 25.0%
!! TAATCTTGTTATAATGAGTG+TGG + chr3.2:70159080-70159099 None:intergenic 25.0%
!! TGTAAATTTACCTTGCATTT+TGG + chr3.2:70158866-70158885 None:intergenic 25.0%
!! TTACGTGTAGAAATTTTGTA+AGG - chr3.2:70160172-70160191 MS.gene25829:intron 25.0%
!!! TTCAATGTTTTGGAATTTGT+TGG + chr3.2:70159536-70159555 None:intergenic 25.0%
!!! TTCGTTTTTTATTGGTTTAG+TGG + chr3.2:70159396-70159415 None:intergenic 25.0%
AAACGTTCAAAGTTGGATAA+AGG - chr3.2:70159742-70159761 MS.gene25829:intron 30.0%
AACGTTCAAAGTTGGATAAA+GGG - chr3.2:70159743-70159762 MS.gene25829:intron 30.0%
AGTCATTCAGTTAATACACA+AGG - chr3.2:70158814-70158833 MS.gene25829:CDS 30.0%
CTTATCCCATTAAATTGGAT+CGG - chr3.2:70159992-70160011 MS.gene25829:intron 30.0%
GTCATTCAGTTAATACACAA+GGG - chr3.2:70158815-70158834 MS.gene25829:CDS 30.0%
! CTGTTGTTGAACTTTAGAAA+TGG + chr3.2:70159631-70159650 None:intergenic 30.0%
! CTTTTCTACCAATCCAAATA+TGG - chr3.2:70158941-70158960 MS.gene25829:intron 30.0%
! GATTTGTGGTTTCAATGTTT+TGG + chr3.2:70159546-70159565 None:intergenic 30.0%
! GTATACCAACATCTTTTGTT+AGG + chr3.2:70158844-70158863 None:intergenic 30.0%
!! CATATTTGGATTGGTAGAAA+AGG + chr3.2:70158943-70158962 None:intergenic 30.0%
!!! GTTTTGGAATTTGTTGGTTA+TGG + chr3.2:70159530-70159549 None:intergenic 30.0%
AACAAACTCCGAACCATATT+TGG + chr3.2:70158957-70158976 None:intergenic 35.0%
ACACATACACTGCAAATGAT+TGG - chr3.2:70159483-70159502 MS.gene25829:CDS 35.0%
ACTATACTCAACCAGATCTA+AGG + chr3.2:70160347-70160366 None:intergenic 35.0%
ATATTTCAAGCCCTTAGATC+TGG - chr3.2:70160333-70160352 MS.gene25829:CDS 35.0%
ATGTTGCCGATCCAATTTAA+TGG + chr3.2:70160001-70160020 None:intergenic 35.0%
CAACACATGCTATTAAGAAC+CGG + chr3.2:70159334-70159353 None:intergenic 35.0%
CAATTTAATGGGATAAGGCT+TGG + chr3.2:70159989-70160008 None:intergenic 35.0%
CGATCCAATTTAATGGGATA+AGG + chr3.2:70159994-70160013 None:intergenic 35.0%
CTACCAATCCAAATATGGTT+CGG - chr3.2:70158946-70158965 MS.gene25829:intron 35.0%
CTATACTCAACCAGATCTAA+GGG + chr3.2:70160346-70160365 None:intergenic 35.0%
CTTTGTGATACCACAAGAAA+GGG + chr3.2:70159608-70159627 None:intergenic 35.0%
GCTTGAAAAACGTTCAAAGT+TGG - chr3.2:70159735-70159754 MS.gene25829:intron 35.0%
GGAGCTTCGATGATAATAAT+GGG + chr3.2:70159772-70159791 None:intergenic 35.0%
TATGTGTGAGGAAGGATATT+AGG + chr3.2:70159470-70159489 None:intergenic 35.0%
TGAATCAAAAGAGTCATCAG+AGG - chr3.2:70159702-70159721 MS.gene25829:intron 35.0%
TGGAGCTTCGATGATAATAA+TGG + chr3.2:70159773-70159792 None:intergenic 35.0%
TGTTGCCGATCCAATTTAAT+GGG + chr3.2:70160000-70160019 None:intergenic 35.0%
TGTTGGTATACCAAAATGCA+AGG - chr3.2:70158853-70158872 MS.gene25829:CDS 35.0%
! CAAGCCTTATCCCATTAAAT+TGG - chr3.2:70159987-70160006 MS.gene25829:intron 35.0%
! GTTGCAATGTTTCTGATTTG+TGG + chr3.2:70159560-70159579 None:intergenic 35.0%
!!! AGCATGTGTTGTTTTGTAGT+AGG - chr3.2:70159343-70159362 MS.gene25829:CDS 35.0%
AAATACCTTCCAACATCACC+AGG + chr3.2:70159856-70159875 None:intergenic 40.0%
AAGAAAGCCCAAAGATGTGT+GGG - chr3.2:70160270-70160289 MS.gene25829:intron 40.0%
ACAGCTTGACGTATCAACAA+CGG - chr3.2:70159647-70159666 MS.gene25829:intron 40.0%
ACTCCGAACCATATTTGGAT+TGG + chr3.2:70158952-70158971 None:intergenic 40.0%
ATGTCTTTGTCCCTTTCTTG+TGG - chr3.2:70159595-70159614 MS.gene25829:CDS 40.0%
CTAAGCCAGAATTAACTCTG+AGG + chr3.2:70159828-70159847 None:intergenic 40.0%
GCTTTGTGATACCACAAGAA+AGG + chr3.2:70159609-70159628 None:intergenic 40.0%
GGAAGCCTAACAAAAGATGT+TGG - chr3.2:70158836-70158855 MS.gene25829:CDS 40.0%
GTCACTGTGAAACAGAATGA+AGG + chr3.2:70159374-70159393 None:intergenic 40.0%
TCATTTGCAGTGTATGTGTG+AGG + chr3.2:70159482-70159501 None:intergenic 40.0%
! TTAGTCAAACCTGGTGATGT+TGG - chr3.2:70159844-70159863 MS.gene25829:intron 40.0%
!! AATTCTGGCTTAGTCAAACC+TGG - chr3.2:70159835-70159854 MS.gene25829:intron 40.0%
!! CAGAATTAACTCTGAGGCAA+GGG + chr3.2:70159822-70159841 None:intergenic 40.0%
!! GCTGCAGTTTTGATCATCTT+TGG + chr3.2:70159793-70159812 None:intergenic 40.0%
CAAGAAAGCCCAAAGATGTG+TGG - chr3.2:70160269-70160288 MS.gene25829:intron 45.0%
CCTTGCCTCAGAGTTAATTC+TGG - chr3.2:70159820-70159839 MS.gene25829:intron 45.0%
TTGCAGTGTATGTGTGAGGA+AGG + chr3.2:70159478-70159497 None:intergenic 45.0%
! TCAAACCTGGTGATGTTGGA+AGG - chr3.2:70159848-70159867 MS.gene25829:intron 45.0%
!! CCAGAATTAACTCTGAGGCA+AGG + chr3.2:70159823-70159842 None:intergenic 45.0%
!!! AAATATGATTATAATTTTTT+TGG + chr3.2:70159179-70159198 None:intergenic 5.0%
!!! AATATGATTATAATTTTTTT+GGG + chr3.2:70159178-70159197 None:intergenic 5.0%
!!! TTATTCAAAAATATTTTTTA+TGG + chr3.2:70159044-70159063 None:intergenic 5.0%
CCAAAGATGTGTGGGCAGTA+CGG - chr3.2:70160278-70160297 MS.gene25829:intron 50.0%
CGTACTGCCCACACATCTTT+GGG + chr3.2:70160280-70160299 None:intergenic 50.0%
GCTTGACGTATCAACAACGG+AGG - chr3.2:70159650-70159669 MS.gene25829:intron 50.0%
CCGTACTGCCCACACATCTT+TGG + chr3.2:70160281-70160300 None:intergenic 55.0%
TGGGCAGTACGGCTCAACAT+TGG - chr3.2:70160289-70160308 MS.gene25829:intron 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 70158811 70160369 70158811 ID=MS.gene25829
chr3.2 mRNA 70158811 70160369 70158811 ID=MS.gene25829.t1;Parent=MS.gene25829
chr3.2 exon 70160306 70160369 70160306 ID=MS.gene25829.t1.exon1;Parent=MS.gene25829.t1
chr3.2 CDS 70160306 70160369 70160306 ID=cds.MS.gene25829.t1;Parent=MS.gene25829.t1
chr3.2 exon 70159311 70159653 70159311 ID=MS.gene25829.t1.exon2;Parent=MS.gene25829.t1
chr3.2 CDS 70159311 70159653 70159311 ID=cds.MS.gene25829.t1;Parent=MS.gene25829.t1
chr3.2 exon 70158811 70158919 70158811 ID=MS.gene25829.t1.exon3;Parent=MS.gene25829.t1
chr3.2 CDS 70158811 70158919 70158811 ID=cds.MS.gene25829.t1;Parent=MS.gene25829.t1
Gene Sequence

>MS.gene25829

ATGAGTCATTCAGTTAATACACAAGGGAAGCCTAACAAAAGATGTTGGTATACCAAAATGCAAGGTAAATTTACAGGAAGTTATTTAAAACAACTCTTAACAATTTAAACTGATTTTATTTCATCATATCCTTTTCTACCAATCCAAATATGGTTCGGAGTTTGTTTATTTGATATATTAATATATAATATTCATGTAAAATAGAAGTTAAATGTACTTGTTATGTTTGACCATAAAAAATATTTTTGAATAATTTTTCTCATTTACCACACTCATTATAACAAGATTATTTCTTTTTGAGAGAACAAGATTATTTCTTAAAAGTTATTCAATTAGTTTTTCATTATAATCATATTTCATCCTTCCCAAAAAAATTATAATCATATTTCAGATACATGTTTTTGTTCAATTTTTTTAAAAAATACATTGAAAATTGTTAAAAACAATTATTTTTAAAAAGATAAACTTTTCTATTTTTTTTGAGGCAAGATAAACTTTTCTATAGAAACTATGAGTTAAACCGGTTCTTAATAGCATGTGTTGTTTTGTAGTAGGCGTTTCCTTCATTCTGTTTCACAGTGACCACTAAACCAATAAAAAACGAAACAAAAATAACATTATCTAAACAAATTTTATCTTTTACAATATCTGTCTATCCTAATATCCTTCCTCACACATACACTGCAAATGATTGGAGCTTTGACAAACACTTTTAGCCATAACCAACAAATTCCAAAACATTGAAACCACAAATCAGAAACATTGCAACGTTCAATCTTTAAGCATGTCTTTGTCCCTTTCTTGTGGTATCACAAAGCCATTTCTAAAGTTCAACAACAGCTTGACGTATCAACAACGGAGGCCAAATATTTCAGTGTCATTAGTAAGGTGTGAATCAAAAGAGTCATCAGAGGAGAATGAGTTGCTTGAAAAACGTTCAAAGTTGGATAAAGGGTCTCCCATTATTATCATCGAAGCTCCAAAGATGATCAAAACTGCAGCTTCTGTCCCTTGCCTCAGAGTTAATTCTGGCTTAGTCAAACCTGGTGATGTTGGAAGGTATTTCACCTCATCTTTTAAGTTTTTCATTTTATCAATTTTTTTCTTAATCTTCAATTTTTTGCATGACTATATGATTTTGTGAGAATCAAATCAAATGCTTACGACAACAATTACCAAGCCTTATCCCATTAAATTGGATCGGCAACATATATCAGATTACACAATAATGCTCTATCAAATGTATAAATGATTTAATTTGGTAAAACCTTTTTTACTATGTATCTAATCAAATCATTTCATAATGTTGATCTTTATTATTAATTTCTACAATATCTCTATTTGACATGATTTGATTGAATTTACGTGTAGAAATTTTGTAAGGTGCATATAAATTAATATCATGTATAAAATGGCAATGGTGTAAATAATTATATATCACTTGGTGCAGAATTGTTTCAAGAAAGCCCAAAGATGTGTGGGCAGTACGGCTCAACATTGGAACTTATCTTATTGATGGCAAATATTTCAAGCCCTTAGATCTGGTTGAGTATAGTTAG

Protein sequence

>MS.gene25829.t1

MSHSVNTQGKPNKRCWYTKMQAITNKFQNIETTNQKHCNVQSLSMSLSLSCGITKPFLKFNNSLTYQQRRPNISVSLVRCESKESSEENELLEKRSKLDKGSPIIIIEAPKMIKTAASVPCLRVNSGLVKPGDVGRIVSRKPKDVWAVRLNIGTYLIDGKYFKPLDLVEYS