Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27835.t1 | XP_003629835.1 | 95.6 | 225 | 9 | 1 | 1 | 225 | 2 | 225 | 9.10E-123 | 449.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27835.t1 | P32110 | 47.5 | 221 | 112 | 3 | 4 | 222 | 6 | 224 | 6.5e-53 | 208.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27835.t1 | G7L788 | 95.6 | 225 | 9 | 1 | 1 | 225 | 2 | 225 | 6.5e-123 | 449.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049259 | MS.gene27835 | 0.807185 | 5.72E-50 | -1.69E-46 |
| MS.gene049281 | MS.gene27835 | 0.822564 | 2.21E-53 | -1.69E-46 |
| MS.gene049611 | MS.gene27835 | 0.805124 | 1.55E-49 | -1.69E-46 |
| MS.gene050363 | MS.gene27835 | 0.807573 | 4.73E-50 | -1.69E-46 |
| MS.gene050381 | MS.gene27835 | 0.856465 | 3.16E-62 | -1.69E-46 |
| MS.gene050883 | MS.gene27835 | 0.874256 | 7.78E-68 | -1.69E-46 |
| MS.gene051044 | MS.gene27835 | 0.828067 | 1.10E-54 | -1.69E-46 |
| MS.gene051127 | MS.gene27835 | 0.865989 | 3.96E-65 | -1.69E-46 |
| MS.gene051138 | MS.gene27835 | 0.811676 | 6.22E-51 | -1.69E-46 |
| MS.gene051364 | MS.gene27835 | 0.817539 | 3.13E-52 | -1.69E-46 |
| MS.gene051366 | MS.gene27835 | 0.802833 | 4.65E-49 | -1.69E-46 |
| MS.gene05141 | MS.gene27835 | 0.811978 | 5.34E-51 | -1.69E-46 |
| MS.gene051946 | MS.gene27835 | 0.838362 | 3.01E-57 | -1.69E-46 |
| MS.gene052089 | MS.gene27835 | 0.808218 | 3.45E-50 | -1.69E-46 |
| MS.gene052478 | MS.gene27835 | 0.817459 | 3.26E-52 | -1.69E-46 |
| MS.gene053069 | MS.gene27835 | 0.805235 | 1.47E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene27835 | 0.866649 | 2.44E-65 | -1.69E-46 |
| MS.gene053488 | MS.gene27835 | 0.818223 | 2.19E-52 | -1.69E-46 |
| MS.gene053549 | MS.gene27835 | 0.820257 | 7.55E-53 | -1.69E-46 |
| MS.gene053561 | MS.gene27835 | 0.827991 | 1.15E-54 | -1.69E-46 |
| MS.gene053826 | MS.gene27835 | 0.855497 | 6.06E-62 | -1.69E-46 |
| MS.gene054056 | MS.gene27835 | 0.820881 | 5.43E-53 | -1.69E-46 |
| MS.gene054708 | MS.gene27835 | 0.825288 | 5.09E-54 | -1.69E-46 |
| MS.gene055124 | MS.gene27835 | 0.834054 | 3.74E-56 | -1.69E-46 |
| MS.gene055154 | MS.gene27835 | 0.856852 | 2.43E-62 | -1.69E-46 |
| MS.gene055202 | MS.gene27835 | 0.831223 | 1.89E-55 | -1.69E-46 |
| MS.gene055549 | MS.gene27835 | 0.839092 | 1.95E-57 | -1.69E-46 |
| MS.gene055550 | MS.gene27835 | 0.83779 | 4.22E-57 | -1.69E-46 |
| MS.gene055551 | MS.gene27835 | 0.826259 | 2.99E-54 | -1.69E-46 |
| MS.gene055942 | MS.gene27835 | 0.812669 | 3.77E-51 | -1.69E-46 |
| MS.gene056310 | MS.gene27835 | 0.846029 | 2.81E-59 | -1.69E-46 |
| MS.gene056311 | MS.gene27835 | 0.880492 | 5.25E-70 | -1.69E-46 |
| MS.gene056313 | MS.gene27835 | 0.822182 | 2.72E-53 | -1.69E-46 |
| MS.gene056408 | MS.gene27835 | 0.809751 | 1.62E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene27835 | -0.817504 | 3.19E-52 | -1.69E-46 |
| MS.gene056530 | MS.gene27835 | 0.805019 | 1.63E-49 | -1.69E-46 |
| MS.gene057014 | MS.gene27835 | 0.8642 | 1.44E-64 | -1.69E-46 |
| MS.gene057499 | MS.gene27835 | 0.805538 | 1.27E-49 | -1.69E-46 |
| MS.gene058068 | MS.gene27835 | 0.825065 | 5.74E-54 | -1.69E-46 |
| MS.gene058099 | MS.gene27835 | 0.837816 | 4.16E-57 | -1.69E-46 |
| MS.gene058108 | MS.gene27835 | 0.809468 | 1.86E-50 | -1.69E-46 |
| MS.gene058109 | MS.gene27835 | 0.855059 | 8.13E-62 | -1.69E-46 |
| MS.gene058111 | MS.gene27835 | 0.840186 | 1.01E-57 | -1.69E-46 |
| MS.gene058171 | MS.gene27835 | 0.866826 | 2.15E-65 | -1.69E-46 |
| MS.gene058173 | MS.gene27835 | 0.859891 | 3.02E-63 | -1.69E-46 |
| MS.gene058175 | MS.gene27835 | 0.866235 | 3.31E-65 | -1.69E-46 |
| MS.gene058268 | MS.gene27835 | 0.819299 | 1.25E-52 | -1.69E-46 |
| MS.gene058269 | MS.gene27835 | 0.805079 | 1.59E-49 | -1.69E-46 |
| MS.gene058493 | MS.gene27835 | 0.955908 | 1.02E-113 | -1.69E-46 |
| MS.gene058494 | MS.gene27835 | 0.925308 | 2.19E-90 | -1.69E-46 |
| MS.gene058496 | MS.gene27835 | 0.920102 | 1.96E-87 | -1.69E-46 |
| MS.gene058628 | MS.gene27835 | 0.860838 | 1.56E-63 | -1.69E-46 |
| MS.gene058629 | MS.gene27835 | 0.832698 | 8.15E-56 | -1.69E-46 |
| MS.gene058630 | MS.gene27835 | 0.856974 | 2.24E-62 | -1.69E-46 |
| MS.gene059022 | MS.gene27835 | -0.814056 | 1.87E-51 | -1.69E-46 |
| MS.gene059045 | MS.gene27835 | 0.819877 | 9.22E-53 | -1.69E-46 |
| MS.gene059223 | MS.gene27835 | 0.828726 | 7.66E-55 | -1.69E-46 |
| MS.gene059690 | MS.gene27835 | 0.806911 | 6.54E-50 | -1.69E-46 |
| MS.gene059694 | MS.gene27835 | 0.801378 | 9.26E-49 | -1.69E-46 |
| MS.gene059697 | MS.gene27835 | 0.801781 | 7.66E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene27835 | -0.820754 | 5.80E-53 | -1.69E-46 |
| MS.gene059916 | MS.gene27835 | 0.816232 | 6.15E-52 | -1.69E-46 |
| MS.gene060595 | MS.gene27835 | 0.852116 | 5.70E-61 | -1.69E-46 |
| MS.gene06077 | MS.gene27835 | -0.817963 | 2.51E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27835.t1 | MTR_8g087410 | 95.556 | 225 | 9 | 1 | 1 | 225 | 2 | 225 | 4.02e-158 | 437 |
| MS.gene27835.t1 | MTR_8g087425 | 63.839 | 224 | 80 | 1 | 1 | 224 | 1 | 223 | 2.46e-95 | 278 |
| MS.gene27835.t1 | MTR_6g080440 | 52.885 | 208 | 95 | 2 | 5 | 211 | 3 | 208 | 1.63e-71 | 217 |
| MS.gene27835.t1 | MTR_7g065720 | 48.113 | 212 | 106 | 3 | 2 | 211 | 4 | 213 | 1.31e-63 | 197 |
| MS.gene27835.t1 | MTR_4g019780 | 49.048 | 210 | 106 | 1 | 2 | 211 | 5 | 213 | 1.98e-62 | 194 |
| MS.gene27835.t1 | MTR_7g065740 | 43.111 | 225 | 124 | 3 | 2 | 224 | 4 | 226 | 8.73e-62 | 192 |
| MS.gene27835.t1 | MTR_1g090150 | 46.330 | 218 | 113 | 3 | 4 | 219 | 2 | 217 | 7.22e-60 | 188 |
| MS.gene27835.t1 | MTR_3g099757 | 46.890 | 209 | 110 | 1 | 1 | 209 | 1 | 208 | 9.85e-60 | 187 |
| MS.gene27835.t1 | MTR_7g065590 | 45.370 | 216 | 115 | 3 | 1 | 215 | 3 | 216 | 1.30e-59 | 187 |
| MS.gene27835.t1 | MTR_4g019790 | 45.249 | 221 | 118 | 3 | 5 | 224 | 7 | 225 | 4.12e-59 | 186 |
| MS.gene27835.t1 | MTR_7g065660 | 44.186 | 215 | 116 | 4 | 3 | 215 | 4 | 216 | 1.11e-58 | 185 |
| MS.gene27835.t1 | MTR_7g065750 | 43.556 | 225 | 123 | 3 | 2 | 224 | 4 | 226 | 3.69e-58 | 183 |
| MS.gene27835.t1 | MTR_7g065600 | 46.512 | 215 | 112 | 3 | 2 | 215 | 4 | 216 | 1.59e-57 | 182 |
| MS.gene27835.t1 | MTR_1g115195 | 45.413 | 218 | 115 | 3 | 5 | 219 | 3 | 219 | 3.11e-56 | 178 |
| MS.gene27835.t1 | MTR_7g065630 | 41.892 | 222 | 122 | 4 | 4 | 224 | 24 | 239 | 9.91e-56 | 177 |
| MS.gene27835.t1 | MTR_7g065290 | 45.540 | 213 | 113 | 3 | 4 | 215 | 6 | 216 | 1.10e-55 | 177 |
| MS.gene27835.t1 | MTR_7g065230 | 44.091 | 220 | 115 | 4 | 4 | 222 | 6 | 218 | 3.33e-54 | 173 |
| MS.gene27835.t1 | MTR_1g090060 | 43.636 | 220 | 121 | 2 | 5 | 222 | 3 | 221 | 1.58e-53 | 172 |
| MS.gene27835.t1 | MTR_7g065265 | 44.037 | 218 | 117 | 5 | 4 | 219 | 6 | 220 | 5.07e-53 | 170 |
| MS.gene27835.t1 | MTR_7g065710 | 44.444 | 207 | 108 | 4 | 6 | 211 | 5 | 205 | 1.24e-52 | 169 |
| MS.gene27835.t1 | MTR_7g065270 | 43.318 | 217 | 120 | 3 | 4 | 219 | 6 | 220 | 2.71e-51 | 166 |
| MS.gene27835.t1 | MTR_7g065680 | 38.117 | 223 | 131 | 4 | 3 | 224 | 5 | 221 | 9.72e-51 | 164 |
| MS.gene27835.t1 | MTR_1g090070 | 42.791 | 215 | 119 | 3 | 1 | 212 | 5 | 218 | 1.06e-50 | 164 |
| MS.gene27835.t1 | MTR_5g040430 | 41.315 | 213 | 118 | 3 | 3 | 209 | 5 | 216 | 1.86e-50 | 164 |
| MS.gene27835.t1 | MTR_2g070150 | 45.098 | 204 | 108 | 3 | 5 | 207 | 4 | 204 | 2.68e-50 | 162 |
| MS.gene27835.t1 | MTR_7g065260 | 45.701 | 221 | 107 | 5 | 4 | 218 | 13 | 226 | 4.81e-50 | 163 |
| MS.gene27835.t1 | MTR_4g059730 | 43.172 | 227 | 111 | 5 | 1 | 218 | 1 | 218 | 1.01e-49 | 162 |
| MS.gene27835.t1 | MTR_7g065640 | 41.395 | 215 | 104 | 4 | 3 | 215 | 4 | 198 | 3.83e-49 | 160 |
| MS.gene27835.t1 | MTR_2g070120 | 43.694 | 222 | 117 | 4 | 5 | 225 | 4 | 218 | 8.40e-49 | 159 |
| MS.gene27835.t1 | MTR_2g070180 | 44.118 | 204 | 110 | 3 | 6 | 208 | 5 | 205 | 7.54e-48 | 157 |
| MS.gene27835.t1 | MTR_2g070200 | 39.815 | 216 | 126 | 3 | 5 | 218 | 4 | 217 | 5.63e-47 | 155 |
| MS.gene27835.t1 | MTR_2g070140 | 45.026 | 191 | 101 | 3 | 19 | 208 | 2 | 189 | 2.72e-46 | 152 |
| MS.gene27835.t1 | MTR_1g090090 | 40.278 | 216 | 126 | 2 | 5 | 218 | 3 | 217 | 8.73e-46 | 152 |
| MS.gene27835.t1 | MTR_1g090100 | 42.788 | 208 | 116 | 2 | 6 | 211 | 3 | 209 | 9.88e-46 | 151 |
| MS.gene27835.t1 | MTR_7g065700 | 42.927 | 205 | 110 | 4 | 4 | 207 | 6 | 204 | 2.30e-45 | 150 |
| MS.gene27835.t1 | MTR_2g070130 | 40.488 | 205 | 118 | 3 | 5 | 208 | 4 | 205 | 4.48e-45 | 150 |
| MS.gene27835.t1 | MTR_5g037380 | 38.182 | 220 | 127 | 4 | 1 | 212 | 1 | 219 | 1.68e-44 | 149 |
| MS.gene27835.t1 | MTR_2g070210 | 44.385 | 187 | 97 | 5 | 5 | 187 | 4 | 187 | 4.52e-43 | 145 |
| MS.gene27835.t1 | MTR_3g467420 | 45.109 | 184 | 98 | 2 | 5 | 187 | 9 | 190 | 5.02e-43 | 145 |
| MS.gene27835.t1 | MTR_2g070060 | 41.935 | 186 | 103 | 4 | 4 | 187 | 3 | 185 | 5.63e-42 | 142 |
| MS.gene27835.t1 | MTR_8g061950 | 41.014 | 217 | 119 | 6 | 1 | 209 | 1 | 216 | 9.02e-42 | 142 |
| MS.gene27835.t1 | MTR_8g056940 | 42.021 | 188 | 106 | 2 | 4 | 190 | 3 | 188 | 1.82e-41 | 140 |
| MS.gene27835.t1 | MTR_4g124130 | 45.405 | 185 | 97 | 3 | 5 | 187 | 4 | 186 | 5.00e-41 | 139 |
| MS.gene27835.t1 | MTR_3g467430 | 43.085 | 188 | 104 | 2 | 1 | 187 | 5 | 190 | 2.55e-39 | 135 |
| MS.gene27835.t1 | MTR_2g070070 | 40.000 | 210 | 118 | 6 | 4 | 211 | 3 | 206 | 4.06e-37 | 129 |
| MS.gene27835.t1 | MTR_2g070110 | 41.622 | 185 | 104 | 3 | 4 | 187 | 3 | 184 | 1.30e-36 | 128 |
| MS.gene27835.t1 | MTR_5g076900 | 39.037 | 187 | 111 | 2 | 2 | 187 | 3 | 187 | 2.12e-36 | 127 |
| MS.gene27835.t1 | MTR_0186s0030 | 50.000 | 130 | 64 | 1 | 8 | 136 | 7 | 136 | 2.72e-33 | 117 |
| MS.gene27835.t1 | MTR_2g072120 | 48.649 | 74 | 34 | 3 | 48 | 118 | 1 | 73 | 2.41e-17 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27835.t1 | AT2G29420 | 44.037 | 218 | 121 | 1 | 3 | 220 | 7 | 223 | 6.97e-64 | 198 |
| MS.gene27835.t1 | AT2G29460 | 46.188 | 223 | 116 | 3 | 2 | 222 | 4 | 224 | 1.91e-62 | 194 |
| MS.gene27835.t1 | AT2G29490 | 48.571 | 210 | 104 | 3 | 2 | 209 | 4 | 211 | 5.50e-62 | 193 |
| MS.gene27835.t1 | AT2G29480 | 45.740 | 223 | 117 | 3 | 2 | 222 | 4 | 224 | 5.55e-62 | 193 |
| MS.gene27835.t1 | AT2G29450 | 46.847 | 222 | 113 | 4 | 4 | 222 | 5 | 224 | 1.41e-60 | 189 |
| MS.gene27835.t1 | AT2G29440 | 45.740 | 223 | 117 | 3 | 2 | 222 | 3 | 223 | 6.97e-60 | 188 |
| MS.gene27835.t1 | AT3G09270 | 46.667 | 210 | 109 | 3 | 2 | 209 | 3 | 211 | 2.05e-58 | 184 |
| MS.gene27835.t1 | AT2G29470 | 45.498 | 211 | 110 | 4 | 2 | 209 | 4 | 212 | 2.02e-55 | 176 |
| MS.gene27835.t1 | AT1G17170 | 42.791 | 215 | 118 | 4 | 5 | 218 | 4 | 214 | 1.88e-49 | 161 |
| MS.gene27835.t1 | AT1G59670 | 43.662 | 213 | 111 | 5 | 4 | 208 | 5 | 216 | 1.24e-47 | 157 |
| MS.gene27835.t1 | AT1G17180 | 41.148 | 209 | 119 | 3 | 5 | 212 | 4 | 209 | 2.20e-47 | 156 |
| MS.gene27835.t1 | AT1G10370 | 42.081 | 221 | 120 | 4 | 1 | 214 | 1 | 220 | 4.49e-47 | 155 |
| MS.gene27835.t1 | AT1G59700 | 43.564 | 202 | 106 | 4 | 4 | 197 | 5 | 206 | 6.17e-46 | 152 |
| MS.gene27835.t1 | AT1G78370 | 39.806 | 206 | 120 | 3 | 8 | 212 | 7 | 209 | 1.64e-45 | 151 |
| MS.gene27835.t1 | AT1G17190 | 40.359 | 223 | 129 | 3 | 1 | 222 | 1 | 220 | 3.89e-45 | 150 |
| MS.gene27835.t1 | AT1G78380 | 42.000 | 200 | 103 | 3 | 4 | 202 | 3 | 190 | 4.89e-45 | 150 |
| MS.gene27835.t1 | AT1G78340 | 43.169 | 183 | 101 | 3 | 5 | 186 | 4 | 184 | 3.15e-44 | 147 |
| MS.gene27835.t1 | AT1G53680 | 43.617 | 188 | 101 | 3 | 3 | 187 | 5 | 190 | 2.84e-43 | 145 |
| MS.gene27835.t1 | AT1G74590 | 38.164 | 207 | 122 | 3 | 13 | 214 | 15 | 220 | 2.69e-42 | 143 |
| MS.gene27835.t1 | AT1G78360 | 39.908 | 218 | 126 | 5 | 5 | 220 | 4 | 218 | 8.03e-42 | 141 |
| MS.gene27835.t1 | AT1G78320 | 38.426 | 216 | 127 | 4 | 4 | 216 | 3 | 215 | 9.85e-42 | 141 |
| MS.gene27835.t1 | AT3G43800 | 41.627 | 209 | 115 | 4 | 1 | 204 | 1 | 207 | 1.88e-41 | 141 |
| MS.gene27835.t1 | AT1G27130 | 37.500 | 224 | 131 | 6 | 2 | 217 | 3 | 225 | 2.05e-40 | 138 |
| MS.gene27835.t1 | AT5G62480 | 37.037 | 216 | 125 | 4 | 14 | 220 | 17 | 230 | 3.73e-40 | 138 |
| MS.gene27835.t1 | AT1G10360 | 38.725 | 204 | 118 | 3 | 1 | 197 | 1 | 204 | 2.75e-39 | 135 |
| MS.gene27835.t1 | AT1G69920 | 37.668 | 223 | 126 | 7 | 3 | 215 | 32 | 251 | 1.37e-38 | 134 |
| MS.gene27835.t1 | AT1G27140 | 35.841 | 226 | 135 | 5 | 2 | 218 | 3 | 227 | 7.76e-38 | 132 |
| MS.gene27835.t1 | AT1G69930 | 33.784 | 222 | 140 | 4 | 2 | 217 | 9 | 229 | 1.01e-37 | 131 |
| MS.gene27835.t1 | AT1G78320 | 32.558 | 215 | 114 | 4 | 4 | 216 | 3 | 188 | 6.41e-29 | 107 |
| MS.gene27835.t1 | AT5G62480 | 29.767 | 215 | 116 | 4 | 14 | 220 | 17 | 204 | 1.93e-25 | 99.4 |
| MS.gene27835.t1 | AT5G62480 | 29.767 | 215 | 115 | 4 | 14 | 220 | 17 | 203 | 3.56e-25 | 98.6 |
Find 59 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCAATTTGGGAAGAAAT+TGG | 0.111299 | 4.3:+13513074 | MS.gene27835:CDS |
| GTGACTCTGCAATTGATTTC+TGG | 0.157766 | 4.3:-13511945 | None:intergenic |
| AAAGTTAGAAGAGGAGATAA+AGG | 0.273945 | 4.3:+13512983 | MS.gene27835:CDS |
| GACCCAATTTCTTCCCAAAT+TGG | 0.274164 | 4.3:-13513077 | None:intergenic |
| ATAAAGGGAAAGAAATTCTT+TGG | 0.305857 | 4.3:+13512999 | MS.gene27835:CDS |
| AAAGTTCCGGTTCTTGTTCA+TGG | 0.322486 | 4.3:+13511922 | MS.gene27835:CDS |
| AAAGATCTCAGGCTCGCTTT+TGG | 0.326368 | 4.3:+13512034 | MS.gene27835:CDS |
| TATAGCTTGCGCTGGGTTCA+TGG | 0.334296 | 4.3:+13512895 | MS.gene27835:intron |
| AATGTCCTTCCATCTCTTCA+TGG | 0.338522 | 4.3:+13513123 | MS.gene27835:CDS |
| AGTTAAGCAGCTTCACATCT+TGG | 0.361900 | 4.3:-13511771 | None:intergenic |
| ACCGGAACTTTCTTATGAAT+CGG | 0.380626 | 4.3:-13511910 | None:intergenic |
| TTCCAATTTGGGAAGAAATT+GGG | 0.398944 | 4.3:+13513075 | MS.gene27835:CDS |
| CCCGATTCATAAGAAAGTTC+CGG | 0.414826 | 4.3:+13511909 | MS.gene27835:CDS |
| TTATTGCCACCGTCGCATAA+AGG | 0.420018 | 4.3:+13513214 | MS.gene27835:CDS |
| ACCTTGATCTTGCTCTTGGA+TGG | 0.423990 | 4.3:+13513039 | MS.gene27835:CDS |
| TAAAGAATCATTTCATCCCT+TGG | 0.429286 | 4.3:-13513194 | None:intergenic |
| CCGGAACTTTCTTATGAATC+GGG | 0.433313 | 4.3:-13511909 | None:intergenic |
| TTCTGGCCATGAACAAGAAC+CGG | 0.434336 | 4.3:-13511928 | None:intergenic |
| TGGATTGCATTGATTAAGAA+AGG | 0.435401 | 4.3:+13512915 | MS.gene27835:CDS |
| GGCTAAGTTATCTGATGAAA+AGG | 0.437582 | 4.3:+13512056 | MS.gene27835:CDS |
| CATTCAACTCTACGACCAAC+TGG | 0.446242 | 4.3:-13511805 | None:intergenic |
| GGGTACCTTGATCTTGCTCT+TGG | 0.453072 | 4.3:+13513035 | MS.gene27835:CDS |
| TCTTTGGAGGGGACACTATC+GGG | 0.454808 | 4.3:+13513015 | MS.gene27835:CDS |
| TAAATTATTATAGCTTGCGC+TGG | 0.466026 | 4.3:+13512887 | MS.gene27835:intron |
| TGATTAAGAAAGGTGATGAA+TGG | 0.474122 | 4.3:+13512925 | MS.gene27835:CDS |
| AAAGGAGGCAAGAGAAATCA+TGG | 0.475218 | 4.3:+13512959 | MS.gene27835:CDS |
| TTCTTTGGAGGGGACACTAT+CGG | 0.475345 | 4.3:+13513014 | MS.gene27835:CDS |
| GATTAAGAAAGGTGATGAAT+GGG | 0.480475 | 4.3:+13512926 | MS.gene27835:CDS |
| AAGTTAGAAGAGGAGATAAA+GGG | 0.491500 | 4.3:+13512984 | MS.gene27835:CDS |
| GAAAGGTGATGAATGGGAAA+AGG | 0.495659 | 4.3:+13512932 | MS.gene27835:CDS |
| TGAGATCTTTGATAAGGATC+AGG | 0.498359 | 4.3:-13512021 | None:intergenic |
| AGTTGGTCGTAGAGTTGAAT+GGG | 0.508405 | 4.3:+13511807 | MS.gene27835:CDS |
| CAGTTGGTCGTAGAGTTGAA+TGG | 0.517051 | 4.3:+13511806 | MS.gene27835:CDS |
| TGATCCTTATCAAAGATCTC+AGG | 0.520135 | 4.3:+13512023 | MS.gene27835:CDS |
| CGAGCCTGAGATCTTTGATA+AGG | 0.520914 | 4.3:-13512027 | None:intergenic |
| AAACACTCCTTGATGATGAT+AGG | 0.524604 | 4.3:-13513167 | None:intergenic |
| AATCATGGAAAAGTTAGAAG+AGG | 0.531445 | 4.3:+13512974 | MS.gene27835:CDS |
| AGGGAAAGAAATTCTTTGGA+GGG | 0.536580 | 4.3:+13513003 | MS.gene27835:CDS |
| ATTGATCTTCCATGAAGAGA+TGG | 0.543546 | 4.3:-13513132 | None:intergenic |
| TGAATGGGAAAAGGCTCTAA+AGG | 0.545680 | 4.3:+13512941 | MS.gene27835:CDS |
| TCCATCCAAGAGCAAGATCA+AGG | 0.557485 | 4.3:-13513040 | None:intergenic |
| TAAGGATCAGGAGGCAGCAA+TGG | 0.562517 | 4.3:-13512009 | None:intergenic |
| CAAGGAGTGTTTGCCACCAA+GGG | 0.564625 | 4.3:+13513178 | MS.gene27835:CDS |
| TGAATGGGCTCTAAAACTCA+AGG | 0.568264 | 4.3:+13511822 | MS.gene27835:CDS |
| TCAAGGAGTGTTTGCCACCA+AGG | 0.571195 | 4.3:+13513177 | MS.gene27835:CDS |
| ACTCCTTGATGATGATAGGA+TGG | 0.572678 | 4.3:-13513163 | None:intergenic |
| TTGAATACATTGATGAAACA+TGG | 0.575829 | 4.3:+13511977 | MS.gene27835:CDS |
| AAGGGAAAGAAATTCTTTGG+AGG | 0.576725 | 4.3:+13513002 | MS.gene27835:CDS |
| GAGCCATCCTATCATCATCA+AGG | 0.581782 | 4.3:+13513160 | MS.gene27835:CDS |
| AAATTATTATAGCTTGCGCT+GGG | 0.589664 | 4.3:+13512888 | MS.gene27835:intron |
| AAGAATACTCCTTTATGCGA+CGG | 0.595491 | 4.3:-13513223 | None:intergenic |
| GGGAAAGAAATTCTTTGGAG+GGG | 0.609810 | 4.3:+13513004 | MS.gene27835:CDS |
| ATCTTCCATGAAGAGATGGA+AGG | 0.621011 | 4.3:-13513128 | None:intergenic |
| AGAATCATTTCATCCCTTGG+TGG | 0.643379 | 4.3:-13513191 | None:intergenic |
| GAATGGGCTCTAAAACTCAA+GGG | 0.646090 | 4.3:+13511823 | MS.gene27835:CDS |
| GATCTTTGATAAGGATCAGG+AGG | 0.658191 | 4.3:-13512018 | None:intergenic |
| ATGGGAAAAGGCTCTAAAGG+AGG | 0.677413 | 4.3:+13512944 | MS.gene27835:CDS |
| AATACTCCTTTATGCGACGG+TGG | 0.688837 | 4.3:-13513220 | None:intergenic |
| GAGTATTCTTCAACTCATCG+TGG | 0.696267 | 4.3:+13513236 | MS.gene27835:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAATATAAAACTATTTTAA+AGG | - | chr4.3:13512665-13512684 | None:intergenic | 10.0% |
| !! | TAATTTACACATACAAAAAT+AGG | - | chr4.3:13512874-13512893 | None:intergenic | 15.0% |
| !!! | AATAATTGTTTTATGAACTT+AGG | + | chr4.3:13512272-13512291 | MS.gene27835:intron | 15.0% |
| !! | TATCTCTTGAATTTATTAGA+TGG | - | chr4.3:13512456-13512475 | None:intergenic | 20.0% |
| !! | TATGTTGTTCAAATTTAACT+AGG | + | chr4.3:13512102-13512121 | MS.gene27835:intron | 20.0% |
| !!! | AAAATTATGTTTCCTTTTTG+AGG | + | chr4.3:13512171-13512190 | MS.gene27835:intron | 20.0% |
| ! | AAAATATTCTTGCCTCAAAA+AGG | - | chr4.3:13512186-13512205 | None:intergenic | 25.0% |
| ! | AAATACTACGTTTCTTTCAA+AGG | + | chr4.3:13512142-13512161 | MS.gene27835:intron | 25.0% |
| ! | ATAAAGGGAAAGAAATTCTT+TGG | + | chr4.3:13512999-13513018 | MS.gene27835:CDS | 25.0% |
| ! | ATTACTTGAGTACAATTGTT+TGG | + | chr4.3:13512327-13512346 | MS.gene27835:intron | 25.0% |
| ! | TTGAATACATTGATGAAACA+TGG | + | chr4.3:13511977-13511996 | MS.gene27835:CDS | 25.0% |
| !!! | TTGAGGCAAGAATATTTTTT+TGG | + | chr4.3:13512188-13512207 | MS.gene27835:intron | 25.0% |
| !!! | TTTTTTTGGTGTTAATCATC+CGG | + | chr4.3:13512202-13512221 | MS.gene27835:intron | 25.0% |
| AAAGTTAGAAGAGGAGATAA+AGG | + | chr4.3:13512983-13513002 | MS.gene27835:CDS | 30.0% | |
| AAGTTAGAAGAGGAGATAAA+GGG | + | chr4.3:13512984-13513003 | MS.gene27835:CDS | 30.0% | |
| AATCATGGAAAAGTTAGAAG+AGG | + | chr4.3:13512974-13512993 | MS.gene27835:CDS | 30.0% | |
| ACCGTTTCTATCAACATATT+AGG | - | chr4.3:13512724-13512743 | None:intergenic | 30.0% | |
| ATTTAACTAGGCTTATTCTG+AGG | + | chr4.3:13512114-13512133 | MS.gene27835:intron | 30.0% | |
| CATGATCAATATGTGTTTAG+TGG | + | chr4.3:13512299-13512318 | MS.gene27835:intron | 30.0% | |
| GATGTTAATTATTCCATTCC+AGG | + | chr4.3:13512545-13512564 | MS.gene27835:intron | 30.0% | |
| GATTAAGAAAGGTGATGAAT+GGG | + | chr4.3:13512926-13512945 | MS.gene27835:CDS | 30.0% | |
| TAAAGAATCATTTCATCCCT+TGG | - | chr4.3:13513197-13513216 | None:intergenic | 30.0% | |
| TGATTAAGAAAGGTGATGAA+TGG | + | chr4.3:13512925-13512944 | MS.gene27835:CDS | 30.0% | |
| TGTTAATCATCCGGTTAATA+GGG | + | chr4.3:13512211-13512230 | MS.gene27835:intron | 30.0% | |
| TTCCAATTTGGGAAGAAATT+GGG | + | chr4.3:13513075-13513094 | MS.gene27835:CDS | 30.0% | |
| ! | AAATTATTATAGCTTGCGCT+GGG | + | chr4.3:13512888-13512907 | MS.gene27835:intron | 30.0% |
| ! | AACTTTTTATTGAGTCCAGT+TGG | + | chr4.3:13511790-13511809 | MS.gene27835:CDS | 30.0% |
| ! | GCCTAATATGTTGATAGAAA+CGG | + | chr4.3:13512720-13512739 | MS.gene27835:intron | 30.0% |
| ! | TAAATTATTATAGCTTGCGC+TGG | + | chr4.3:13512887-13512906 | MS.gene27835:intron | 30.0% |
| !! | AGATGGAAGGACATTTTAAT+GGG | - | chr4.3:13513118-13513137 | None:intergenic | 30.0% |
| !! | CACTTGTTTTCTTCCAATTT+GGG | + | chr4.3:13513064-13513083 | MS.gene27835:CDS | 30.0% |
| !! | TCACTTGTTTTCTTCCAATT+TGG | + | chr4.3:13513063-13513082 | MS.gene27835:CDS | 30.0% |
| !! | TGGATTGCATTGATTAAGAA+AGG | + | chr4.3:13512915-13512934 | MS.gene27835:CDS | 30.0% |
| AAGAATACTCCTTTATGCGA+CGG | - | chr4.3:13513226-13513245 | None:intergenic | 35.0% | |
| AAGGGAAAGAAATTCTTTGG+AGG | + | chr4.3:13513002-13513021 | MS.gene27835:CDS | 35.0% | |
| ACAATACTTGAATGTATGCC+TGG | - | chr4.3:13512566-13512585 | None:intergenic | 35.0% | |
| ACCGGAACTTTCTTATGAAT+CGG | - | chr4.3:13511913-13511932 | None:intergenic | 35.0% | |
| AGGGAAAGAAATTCTTTGGA+GGG | + | chr4.3:13513003-13513022 | MS.gene27835:CDS | 35.0% | |
| ATTGATCTTCCATGAAGAGA+TGG | - | chr4.3:13513135-13513154 | None:intergenic | 35.0% | |
| CTTCCAATTTGGGAAGAAAT+TGG | + | chr4.3:13513074-13513093 | MS.gene27835:CDS | 35.0% | |
| GGCTAAGTTATCTGATGAAA+AGG | + | chr4.3:13512056-13512075 | MS.gene27835:CDS | 35.0% | |
| GTGTTAATCATCCGGTTAAT+AGG | + | chr4.3:13512210-13512229 | MS.gene27835:intron | 35.0% | |
| GTTAATCATCCGGTTAATAG+GGG | + | chr4.3:13512212-13512231 | MS.gene27835:intron | 35.0% | |
| TGAGATCTTTGATAAGGATC+AGG | - | chr4.3:13512024-13512043 | None:intergenic | 35.0% | |
| TGATCCTTATCAAAGATCTC+AGG | + | chr4.3:13512023-13512042 | MS.gene27835:CDS | 35.0% | |
| ! | GAGATGGAAGGACATTTTAA+TGG | - | chr4.3:13513119-13513138 | None:intergenic | 35.0% |
| !! | AAACACTCCTTGATGATGAT+AGG | - | chr4.3:13513170-13513189 | None:intergenic | 35.0% |
| AAAGGAGGCAAGAGAAATCA+TGG | + | chr4.3:13512959-13512978 | MS.gene27835:CDS | 40.0% | |
| AAAGTTCCGGTTCTTGTTCA+TGG | + | chr4.3:13511922-13511941 | MS.gene27835:CDS | 40.0% | |
| AATGTCCTTCCATCTCTTCA+TGG | + | chr4.3:13513123-13513142 | MS.gene27835:CDS | 40.0% | |
| ACTTGAATGTATGCCTGGAA+TGG | - | chr4.3:13512561-13512580 | None:intergenic | 40.0% | |
| AGAATCATTTCATCCCTTGG+TGG | - | chr4.3:13513194-13513213 | None:intergenic | 40.0% | |
| AGTTAAGCAGCTTCACATCT+TGG | - | chr4.3:13511774-13511793 | None:intergenic | 40.0% | |
| AGTTGGTCGTAGAGTTGAAT+GGG | + | chr4.3:13511807-13511826 | MS.gene27835:CDS | 40.0% | |
| ATCTTCCATGAAGAGATGGA+AGG | - | chr4.3:13513131-13513150 | None:intergenic | 40.0% | |
| CCCGATTCATAAGAAAGTTC+CGG | + | chr4.3:13511909-13511928 | MS.gene27835:CDS | 40.0% | |
| CCGGAACTTTCTTATGAATC+GGG | - | chr4.3:13511912-13511931 | None:intergenic | 40.0% | |
| GAAAGGTGATGAATGGGAAA+AGG | + | chr4.3:13512932-13512951 | MS.gene27835:CDS | 40.0% | |
| GACCCAATTTCTTCCCAAAT+TGG | - | chr4.3:13513080-13513099 | None:intergenic | 40.0% | |
| GAGTATTCTTCAACTCATCG+TGG | + | chr4.3:13513236-13513255 | MS.gene27835:CDS | 40.0% | |
| GATCTTTGATAAGGATCAGG+AGG | - | chr4.3:13512021-13512040 | None:intergenic | 40.0% | |
| ! | GGGAAAGAAATTCTTTGGAG+GGG | + | chr4.3:13513004-13513023 | MS.gene27835:CDS | 40.0% |
| ! | GTGACTCTGCAATTGATTTC+TGG | - | chr4.3:13511948-13511967 | None:intergenic | 40.0% |
| ! | TGAATGGGAAAAGGCTCTAA+AGG | + | chr4.3:13512941-13512960 | MS.gene27835:CDS | 40.0% |
| !! | ACTCCTTGATGATGATAGGA+TGG | - | chr4.3:13513166-13513185 | None:intergenic | 40.0% |
| !! | GAATGGGCTCTAAAACTCAA+GGG | + | chr4.3:13511823-13511842 | MS.gene27835:CDS | 40.0% |
| !! | TGAATGGGCTCTAAAACTCA+AGG | + | chr4.3:13511822-13511841 | MS.gene27835:CDS | 40.0% |
| AATACTCCTTTATGCGACGG+TGG | - | chr4.3:13513223-13513242 | None:intergenic | 45.0% | |
| ACCTTGATCTTGCTCTTGGA+TGG | + | chr4.3:13513039-13513058 | MS.gene27835:CDS | 45.0% | |
| ACTCTCCTTCCCCTATTAAC+CGG | - | chr4.3:13512224-13512243 | None:intergenic | 45.0% | |
| ATCATCCGGTTAATAGGGGA+AGG | + | chr4.3:13512216-13512235 | MS.gene27835:intron | 45.0% | |
| CAGTTGGTCGTAGAGTTGAA+TGG | + | chr4.3:13511806-13511825 | MS.gene27835:CDS | 45.0% | |
| CATTCAACTCTACGACCAAC+TGG | - | chr4.3:13511808-13511827 | None:intergenic | 45.0% | |
| CGAGCCTGAGATCTTTGATA+AGG | - | chr4.3:13512030-13512049 | None:intergenic | 45.0% | |
| GAGCCATCCTATCATCATCA+AGG | + | chr4.3:13513160-13513179 | MS.gene27835:CDS | 45.0% | |
| TCCATCCAAGAGCAAGATCA+AGG | - | chr4.3:13513043-13513062 | None:intergenic | 45.0% | |
| TTATTGCCACCGTCGCATAA+AGG | + | chr4.3:13513214-13513233 | MS.gene27835:CDS | 45.0% | |
| TTCTGGCCATGAACAAGAAC+CGG | - | chr4.3:13511931-13511950 | None:intergenic | 45.0% | |
| ! | AAAGATCTCAGGCTCGCTTT+TGG | + | chr4.3:13512034-13512053 | MS.gene27835:CDS | 45.0% |
| ! | TTCTTTGGAGGGGACACTAT+CGG | + | chr4.3:13513014-13513033 | MS.gene27835:CDS | 45.0% |
| !! | AAGATCTCAGGCTCGCTTTT+GGG | + | chr4.3:13512035-13512054 | MS.gene27835:CDS | 45.0% |
| !! | ATGGGAAAAGGCTCTAAAGG+AGG | + | chr4.3:13512944-13512963 | MS.gene27835:CDS | 45.0% |
| GGAAGGAGAGTTCTCTCGAA+AGG | + | chr4.3:13512233-13512252 | MS.gene27835:intron | 50.0% | |
| GGGTACCTTGATCTTGCTCT+TGG | + | chr4.3:13513035-13513054 | MS.gene27835:CDS | 50.0% | |
| TAAGGATCAGGAGGCAGCAA+TGG | - | chr4.3:13512012-13512031 | None:intergenic | 50.0% | |
| ! | TATAGCTTGCGCTGGGTTCA+TGG | + | chr4.3:13512895-13512914 | MS.gene27835:intron | 50.0% |
| ! | TCTTTGGAGGGGACACTATC+GGG | + | chr4.3:13513015-13513034 | MS.gene27835:CDS | 50.0% |
| !! | CAAGGAGTGTTTGCCACCAA+GGG | + | chr4.3:13513178-13513197 | MS.gene27835:CDS | 50.0% |
| !! | TCAAGGAGTGTTTGCCACCA+AGG | + | chr4.3:13513177-13513196 | MS.gene27835:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 13511763 | 13513262 | 13511763 | ID=MS.gene27835 |
| chr4.3 | mRNA | 13511763 | 13513262 | 13511763 | ID=MS.gene27835.t1;Parent=MS.gene27835 |
| chr4.3 | exon | 13511763 | 13512077 | 13511763 | ID=MS.gene27835.t1.exon1;Parent=MS.gene27835.t1 |
| chr4.3 | CDS | 13511763 | 13512077 | 13511763 | ID=cds.MS.gene27835.t1;Parent=MS.gene27835.t1 |
| chr4.3 | exon | 13512900 | 13513262 | 13512900 | ID=MS.gene27835.t1.exon2;Parent=MS.gene27835.t1 |
| chr4.3 | CDS | 13512900 | 13513262 | 13512900 | ID=cds.MS.gene27835.t1;Parent=MS.gene27835.t1 |
| Gene Sequence |
| Protein sequence |
