Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30149.t1 | XP_003600055.1 | 89.7 | 311 | 32 | 0 | 1 | 311 | 1 | 311 | 3.10E-161 | 577.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30149.t1 | Q9SZ42 | 58.6 | 302 | 98 | 2 | 26 | 308 | 27 | 320 | 1.5e-103 | 377.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30149.t1 | G7IXK5 | 89.7 | 311 | 32 | 0 | 1 | 311 | 1 | 311 | 2.2e-161 | 577.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene30149 | 0.807966 | 3.90E-50 | -1.69E-46 |
| MS.gene049232 | MS.gene30149 | 0.874636 | 5.79E-68 | -1.69E-46 |
| MS.gene049396 | MS.gene30149 | 0.850954 | 1.22E-60 | -1.69E-46 |
| MS.gene049633 | MS.gene30149 | 0.809384 | 1.94E-50 | -1.69E-46 |
| MS.gene05010 | MS.gene30149 | 0.805686 | 1.18E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene30149 | 0.862332 | 5.46E-64 | -1.69E-46 |
| MS.gene050216 | MS.gene30149 | 0.815162 | 1.06E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene30149 | 0.890871 | 6.65E-74 | -1.69E-46 |
| MS.gene050496 | MS.gene30149 | 0.856348 | 3.42E-62 | -1.69E-46 |
| MS.gene050502 | MS.gene30149 | 0.85468 | 1.05E-61 | -1.69E-46 |
| MS.gene050504 | MS.gene30149 | 0.817958 | 2.52E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene30149 | 0.800317 | 1.53E-48 | -1.69E-46 |
| MS.gene050662 | MS.gene30149 | 0.802278 | 6.05E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene30149 | 0.81442 | 1.55E-51 | -1.69E-46 |
| MS.gene050697 | MS.gene30149 | 0.869742 | 2.47E-66 | -1.69E-46 |
| MS.gene05080 | MS.gene30149 | 0.809593 | 1.75E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene30149 | 0.86054 | 1.92E-63 | -1.69E-46 |
| MS.gene050871 | MS.gene30149 | 0.864832 | 9.16E-65 | -1.69E-46 |
| MS.gene050986 | MS.gene30149 | 0.810945 | 8.96E-51 | -1.69E-46 |
| MS.gene051268 | MS.gene30149 | 0.831352 | 1.75E-55 | -1.69E-46 |
| MS.gene051509 | MS.gene30149 | 0.850521 | 1.61E-60 | -1.69E-46 |
| MS.gene051551 | MS.gene30149 | 0.800632 | 1.32E-48 | -1.69E-46 |
| MS.gene051874 | MS.gene30149 | 0.839826 | 1.26E-57 | -1.69E-46 |
| MS.gene051941 | MS.gene30149 | 0.832954 | 7.04E-56 | -1.69E-46 |
| MS.gene052066 | MS.gene30149 | 0.844215 | 8.68E-59 | -1.69E-46 |
| MS.gene052164 | MS.gene30149 | 0.822345 | 2.49E-53 | -1.69E-46 |
| MS.gene052482 | MS.gene30149 | 0.802667 | 5.03E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene30149 | 0.813359 | 2.66E-51 | -1.69E-46 |
| MS.gene052506 | MS.gene30149 | 0.810247 | 1.27E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene30149 | 0.815205 | 1.04E-51 | -1.69E-46 |
| MS.gene052823 | MS.gene30149 | 0.81665 | 4.96E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene30149 | 0.808811 | 2.58E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene30149 | 0.839483 | 1.54E-57 | -1.69E-46 |
| MS.gene053203 | MS.gene30149 | 0.815065 | 1.12E-51 | -1.69E-46 |
| MS.gene053505 | MS.gene30149 | 0.832252 | 1.05E-55 | -1.69E-46 |
| MS.gene053574 | MS.gene30149 | 0.812087 | 5.06E-51 | -1.69E-46 |
| MS.gene053626 | MS.gene30149 | 0.844645 | 6.65E-59 | -1.69E-46 |
| MS.gene053633 | MS.gene30149 | 0.852734 | 3.80E-61 | -1.69E-46 |
| MS.gene053824 | MS.gene30149 | 0.819814 | 9.53E-53 | -1.69E-46 |
| MS.gene054003 | MS.gene30149 | 0.813035 | 3.14E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene30149 | 0.836428 | 9.41E-57 | -1.69E-46 |
| MS.gene054954 | MS.gene30149 | 0.820134 | 8.05E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene30149 | 0.810026 | 1.41E-50 | -1.69E-46 |
| MS.gene055750 | MS.gene30149 | 0.823175 | 1.60E-53 | -1.69E-46 |
| MS.gene055751 | MS.gene30149 | 0.800675 | 1.29E-48 | -1.69E-46 |
| MS.gene05589 | MS.gene30149 | 0.854011 | 1.64E-61 | -1.69E-46 |
| MS.gene056046 | MS.gene30149 | 0.825819 | 3.81E-54 | -1.69E-46 |
| MS.gene056166 | MS.gene30149 | 0.851781 | 7.10E-61 | -1.69E-46 |
| MS.gene056387 | MS.gene30149 | 0.866005 | 3.91E-65 | -1.69E-46 |
| MS.gene056422 | MS.gene30149 | 0.816106 | 6.56E-52 | -1.69E-46 |
| MS.gene056504 | MS.gene30149 | 0.872942 | 2.16E-67 | -1.69E-46 |
| MS.gene056510 | MS.gene30149 | 0.845407 | 4.14E-59 | -1.69E-46 |
| MS.gene056878 | MS.gene30149 | 0.819729 | 9.97E-53 | -1.69E-46 |
| MS.gene057019 | MS.gene30149 | 0.804491 | 2.11E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene30149 | 0.803228 | 3.85E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene30149 | 0.870405 | 1.50E-66 | -1.69E-46 |
| MS.gene057687 | MS.gene30149 | 0.806352 | 8.58E-50 | -1.69E-46 |
| MS.gene057734 | MS.gene30149 | 0.816091 | 6.61E-52 | -1.69E-46 |
| MS.gene05787 | MS.gene30149 | 0.830588 | 2.70E-55 | -1.69E-46 |
| MS.gene058171 | MS.gene30149 | -0.816336 | 5.83E-52 | -1.69E-46 |
| MS.gene058173 | MS.gene30149 | -0.817722 | 2.85E-52 | -1.69E-46 |
| MS.gene058175 | MS.gene30149 | -0.821067 | 4.92E-53 | -1.69E-46 |
| MS.gene059045 | MS.gene30149 | -0.815001 | 1.16E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene30149 | 0.808389 | 3.17E-50 | -1.69E-46 |
| MS.gene059367 | MS.gene30149 | 0.856685 | 2.72E-62 | -1.69E-46 |
| MS.gene059368 | MS.gene30149 | 0.817117 | 3.89E-52 | -1.69E-46 |
| MS.gene059667 | MS.gene30149 | 0.819027 | 1.44E-52 | -1.69E-46 |
| MS.gene059680 | MS.gene30149 | 0.811869 | 5.64E-51 | -1.69E-46 |
| MS.gene059703 | MS.gene30149 | -0.802885 | 4.54E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene30149 | 0.866087 | 3.68E-65 | -1.69E-46 |
| MS.gene06000 | MS.gene30149 | 0.864316 | 1.33E-64 | -1.69E-46 |
| MS.gene060372 | MS.gene30149 | 0.82152 | 3.87E-53 | -1.69E-46 |
| MS.gene060482 | MS.gene30149 | 0.821008 | 5.07E-53 | -1.69E-46 |
| MS.gene060593 | MS.gene30149 | -0.819532 | 1.11E-52 | -1.69E-46 |
| MS.gene060595 | MS.gene30149 | -0.816937 | 4.28E-52 | -1.69E-46 |
| MS.gene060778 | MS.gene30149 | 0.894351 | 2.68E-75 | -1.69E-46 |
| MS.gene06077 | MS.gene30149 | 0.80004 | 1.74E-48 | -1.69E-46 |
| MS.gene06097 | MS.gene30149 | 0.869858 | 2.26E-66 | -1.69E-46 |
| MS.gene060982 | MS.gene30149 | 0.858139 | 1.01E-62 | -1.69E-46 |
| MS.gene061218 | MS.gene30149 | 0.818725 | 1.69E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene30149 | MS.gene94356 | PPI |
| MS.gene027119 | MS.gene30149 | PPI |
| MS.gene94356 | MS.gene30149 | PPI |
| MS.gene30149 | MS.gene027119 | PPI |
| MS.gene30149 | MS.gene82707 | PPI |
| MS.gene30149 | MS.gene30150 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30149.t1 | MTR_3g051220 | 95.820 | 311 | 13 | 0 | 1 | 311 | 1 | 311 | 0.0 | 597 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30149.t1 | AT4G27030 | 61.513 | 304 | 86 | 2 | 26 | 308 | 27 | 320 | 1.25e-140 | 400 |
| MS.gene30149.t1 | AT1G62190 | 55.674 | 282 | 105 | 3 | 26 | 305 | 25 | 288 | 6.61e-119 | 344 |
| MS.gene30149.t1 | AT2G22890 | 60.938 | 256 | 89 | 2 | 45 | 299 | 34 | 279 | 4.94e-116 | 336 |
Find 104 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATATCCACTGAACCTTTAA+TGG | 0.108550 | 3.3:+42607481 | None:intergenic |
| CATTGGGCCATTGATAATTA+TGG | 0.132929 | 3.3:-42607386 | MS.gene30149:CDS |
| TAGCATTGGGATTGGACTTC+AGG | 0.263938 | 3.3:+42607663 | None:intergenic |
| CCAACAAAACTTTGAAATAT+AGG | 0.275222 | 3.3:+42607203 | None:intergenic |
| TCGTTGGGAGAGTCCATTAA+AGG | 0.296680 | 3.3:-42607494 | MS.gene30149:CDS |
| GGTTACATCTTAGCTGATCT+TGG | 0.300492 | 3.3:-42607422 | MS.gene30149:CDS |
| ATGCAATAATTATTGTTATA+CGG | 0.313977 | 3.3:+42607035 | None:intergenic |
| ATTGGCGGTCGTGCAAGTTT+CGG | 0.325462 | 3.3:+42607623 | None:intergenic |
| TCGATTTGGCCTCCAACTAA+TGG | 0.360766 | 3.3:+42607350 | None:intergenic |
| GATGGACAATGACCCTAGTT+TGG | 0.374790 | 3.3:-42607573 | MS.gene30149:CDS |
| GGCCTTGGAAAGCTTCGATT+TGG | 0.379578 | 3.3:+42607336 | None:intergenic |
| TTGGACTTCAGGGCAGTGGC+TGG | 0.383685 | 3.3:+42607674 | None:intergenic |
| AAAGAAGGGGTCATTAGGTT+TGG | 0.391713 | 3.3:+42606862 | None:intergenic |
| ATTGGGCCATTGATAATTAT+GGG | 0.394460 | 3.3:-42607385 | MS.gene30149:CDS |
| CCAATCCAACCGGCCACAAT+AGG | 0.395624 | 3.3:+42607443 | None:intergenic |
| CTTGTGTCACGGTCGCAACA+TGG | 0.395704 | 3.3:-42607074 | MS.gene30149:CDS |
| GTTACATCTTAGCTGATCTT+GGG | 0.396076 | 3.3:-42607421 | MS.gene30149:CDS |
| GAAAACTTGGTGGGGAAAGC+TGG | 0.399301 | 3.3:+42607760 | None:intergenic |
| CACAACATTGCTCATTTCGT+TGG | 0.401615 | 3.3:-42607510 | MS.gene30149:CDS |
| CAATTTCATGCATGGGCTCA+TGG | 0.404441 | 3.3:-42607149 | MS.gene30149:CDS |
| CGGAGCAATTGACGCAAATT+CGG | 0.404645 | 3.3:+42607717 | None:intergenic |
| CAACAAAACTTTGAAATATA+GGG | 0.407526 | 3.3:+42607204 | None:intergenic |
| CCTATTGTGGCCGGTTGGAT+TGG | 0.416566 | 3.3:-42607443 | MS.gene30149:CDS |
| ATGATCAATTGATTAGCATT+GGG | 0.424942 | 3.3:+42607650 | None:intergenic |
| CAATTGATTAGCATTGGGAT+TGG | 0.436072 | 3.3:+42607655 | None:intergenic |
| TTGGGTCCGGAGTTTACCAT+TGG | 0.438101 | 3.3:-42607403 | MS.gene30149:CDS |
| AATGATCAATTGATTAGCAT+TGG | 0.440879 | 3.3:+42607649 | None:intergenic |
| TTGTGTCACGGTCGCAACAT+GGG | 0.442683 | 3.3:-42607073 | MS.gene30149:CDS |
| TTAAACTTGGGGTTAGGCCT+AGG | 0.445101 | 3.3:-42606941 | MS.gene30149:CDS |
| AATAATTATTGCATAGTAAG+TGG | 0.447132 | 3.3:-42607026 | MS.gene30149:CDS |
| CGCAACATGGGGCACACCAC+CGG | 0.450199 | 3.3:-42607061 | MS.gene30149:CDS |
| ATCTTAGCTGATCTTGGGTC+CGG | 0.450717 | 3.3:-42607416 | MS.gene30149:CDS |
| TGGAGTGTGGAATGAGTTGT+TGG | 0.450873 | 3.3:-42607006 | MS.gene30149:CDS |
| TCGTGGAAAACAAGATCTAT+AGG | 0.454717 | 3.3:+42607227 | None:intergenic |
| ATGGCCACTTATGATGGCCT+TGG | 0.460295 | 3.3:+42607321 | None:intergenic |
| TGCGAGTGCGTGCAAATTGT+TGG | 0.473177 | 3.3:+42607274 | None:intergenic |
| GGGATTGGACTTCAGGGCAG+TGG | 0.481141 | 3.3:+42607670 | None:intergenic |
| ACAACATTGCTCATTTCGTT+GGG | 0.487853 | 3.3:-42607509 | MS.gene30149:CDS |
| GACGAGTCAACACCATTAGT+TGG | 0.493865 | 3.3:-42607362 | MS.gene30149:CDS |
| TAGCCAACAATTTCATGCAT+GGG | 0.501565 | 3.3:-42607156 | MS.gene30149:CDS |
| TCCTCTATCCACTCAGAAGA+AGG | 0.504881 | 3.3:+42606906 | None:intergenic |
| AGCATTGGGATTGGACTTCA+GGG | 0.505486 | 3.3:+42607664 | None:intergenic |
| AAGACACTTGTGTTGGACTA+AGG | 0.508363 | 3.3:+42607784 | None:intergenic |
| GAGCCCATGCATGAAATTGT+TGG | 0.514086 | 3.3:+42607153 | None:intergenic |
| CAACATGGTCTCATCGAGCA+TGG | 0.522244 | 3.3:-42607547 | MS.gene30149:CDS |
| GGCTGGAGTATCAGTGGTAG+TGG | 0.523081 | 3.3:+42607691 | None:intergenic |
| TGGGTCCGGAGTTTACCATT+GGG | 0.524693 | 3.3:-42607402 | MS.gene30149:CDS |
| CTCGTCCCCATAATTATCAA+TGG | 0.530151 | 3.3:+42607379 | None:intergenic |
| ACCTTCTTCTGAGTGGATAG+AGG | 0.532572 | 3.3:-42606907 | MS.gene30149:CDS |
| TGCACGACCGCCAATAAAAG+TGG | 0.536589 | 3.3:-42607615 | MS.gene30149:CDS |
| AATTTCATGCATGGGCTCAT+GGG | 0.536888 | 3.3:-42607148 | MS.gene30149:CDS |
| AATTATTGTTATACGGCGGC+CGG | 0.539022 | 3.3:+42607042 | None:intergenic |
| TGGTCCATGGCCACTTATGA+TGG | 0.539886 | 3.3:+42607315 | None:intergenic |
| AGCTGGGAAGACACTTGTGT+TGG | 0.543194 | 3.3:+42607777 | None:intergenic |
| TAGTGTTCTTTGTGAAAACT+TGG | 0.545737 | 3.3:+42607747 | None:intergenic |
| TAAGATGTAACCAATCCAAC+CGG | 0.546658 | 3.3:+42607433 | None:intergenic |
| CATAATTATCAATGGCCCAA+TGG | 0.547046 | 3.3:+42607387 | None:intergenic |
| CTTTCCAAGGCCATCATAAG+TGG | 0.547599 | 3.3:-42607325 | MS.gene30149:CDS |
| TGGAGCCTATTGTGGCCGGT+TGG | 0.552254 | 3.3:-42607448 | MS.gene30149:CDS |
| AGTATCAGTGGTAGTGGTGG+CGG | 0.553180 | 3.3:+42607697 | None:intergenic |
| CATCGAGCATGGGTGACAGT+TGG | 0.553692 | 3.3:-42607536 | MS.gene30149:CDS |
| TTAGCCAACAATTTCATGCA+TGG | 0.554178 | 3.3:-42607157 | MS.gene30149:CDS |
| AACTTTGAAATATAGGGTCG+TGG | 0.554501 | 3.3:+42607210 | None:intergenic |
| AAGGCCATCATAAGTGGCCA+TGG | 0.554715 | 3.3:-42607319 | MS.gene30149:CDS |
| TATTGTTATACGGCGGCCGG+TGG | 0.555619 | 3.3:+42607045 | None:intergenic |
| GTTCAGTGGATATGAATATG+TGG | 0.556388 | 3.3:-42607472 | MS.gene30149:CDS |
| GACCATGTTGAATCCAAACT+AGG | 0.557252 | 3.3:+42607560 | None:intergenic |
| AAAACTTGGTGGGGAAAGCT+GGG | 0.558716 | 3.3:+42607761 | None:intergenic |
| AACTGATGCTAACCGTCCGA+TGG | 0.562061 | 3.3:-42607591 | MS.gene30149:CDS |
| TTCTTTGTGAAAACTTGGTG+GGG | 0.563589 | 3.3:+42607752 | None:intergenic |
| GGCAAATTGACGCTTAGTGA+TGG | 0.573238 | 3.3:+42607295 | None:intergenic |
| TGTGTCACGGTCGCAACATG+GGG | 0.574793 | 3.3:-42607072 | MS.gene30149:CDS |
| TTGGGGTTAGGCCTAGGTCA+TGG | 0.575041 | 3.3:-42606935 | MS.gene30149:CDS |
| GTTCTTTGTGAAAACTTGGT+GGG | 0.575432 | 3.3:+42607751 | None:intergenic |
| AACATGGTCTCATCGAGCAT+GGG | 0.579331 | 3.3:-42607546 | MS.gene30149:CDS |
| TGCAGAGCTACCACGAGTGG+TGG | 0.582923 | 3.3:+42607110 | None:intergenic |
| TTTGCACGCACTCGCACGCA+TGG | 0.586280 | 3.3:-42607267 | MS.gene30149:CDS |
| TGGGTGGAGCCTATTGTGGC+CGG | 0.588507 | 3.3:-42607452 | MS.gene30149:CDS |
| GGGCTCATGGGACAAAGAGT+AGG | 0.597966 | 3.3:-42607136 | MS.gene30149:CDS |
| TTCAGTGGATATGAATATGT+GGG | 0.598274 | 3.3:-42607471 | MS.gene30149:CDS |
| GGCAGTGGCTGGAGTATCAG+TGG | 0.600643 | 3.3:+42607685 | None:intergenic |
| TGACGCTTAGTGATGGTCCA+TGG | 0.601680 | 3.3:+42607302 | None:intergenic |
| TGTTCTTTGTGAAAACTTGG+TGG | 0.602852 | 3.3:+42607750 | None:intergenic |
| AGCAATTGACGCAAATTCGG+TGG | 0.603113 | 3.3:+42607720 | None:intergenic |
| AGAGTCCATTAAAGGTTCAG+TGG | 0.609489 | 3.3:-42607486 | MS.gene30149:CDS |
| GGCCAAATCGAAGCTTTCCA+AGG | 0.610753 | 3.3:-42607338 | MS.gene30149:CDS |
| ACCATGTTGAATCCAAACTA+GGG | 0.616341 | 3.3:+42607561 | None:intergenic |
| GAGTAGGCTTCCACCACTCG+TGG | 0.618365 | 3.3:-42607120 | MS.gene30149:CDS |
| ATTGCATAGTAAGTGGAGTG+TGG | 0.622359 | 3.3:-42607019 | MS.gene30149:CDS |
| GAAGGTTCACTCCATGACCT+AGG | 0.625361 | 3.3:+42606924 | None:intergenic |
| AAACTAGGGTCATTGTCCAT+CGG | 0.628857 | 3.3:+42607575 | None:intergenic |
| GAGTCAACACCATTAGTTGG+AGG | 0.629904 | 3.3:-42607359 | MS.gene30149:CDS |
| TTGGGCCATTGATAATTATG+GGG | 0.630571 | 3.3:-42607384 | MS.gene30149:CDS |
| ACAGTGGAGTGCTTGTGTCA+CGG | 0.633452 | 3.3:-42607085 | MS.gene30149:CDS |
| TGGAGTATCAGTGGTAGTGG+TGG | 0.638568 | 3.3:+42607694 | None:intergenic |
| ACCCTAGTTTGGATTCAACA+TGG | 0.651337 | 3.3:-42607562 | MS.gene30149:CDS |
| TCTTGCAGAGCTACCACGAG+TGG | 0.651539 | 3.3:+42607107 | None:intergenic |
| AATGGCCCAATGGTAAACTC+CGG | 0.657151 | 3.3:+42607397 | None:intergenic |
| GTGGTAGCTCTGCAAGACAG+TGG | 0.657461 | 3.3:-42607101 | MS.gene30149:CDS |
| TAGGGTCATTGTCCATCGGA+CGG | 0.660969 | 3.3:+42607579 | None:intergenic |
| GGAGTGAACCTTCTTCTGAG+TGG | 0.663604 | 3.3:-42606914 | MS.gene30149:CDS |
| TATGTGGGTGGAGCCTATTG+TGG | 0.671601 | 3.3:-42607456 | MS.gene30149:CDS |
| CAATAATTATTGTTATACGG+CGG | 0.684485 | 3.3:+42607038 | None:intergenic |
| AGTGGATATGAATATGTGGG+TGG | 0.709350 | 3.3:-42607468 | MS.gene30149:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGCAATAATTATTGTTATA+CGG | + | chr3.3:42607634-42607653 | None:intergenic | 15.0% |
| !!! | ATGATATTGTATTTTAAACT+TGG | - | chr3.3:42607712-42607731 | MS.gene30149:CDS | 15.0% |
| !!! | TGATATTGTATTTTAAACTT+GGG | - | chr3.3:42607713-42607732 | MS.gene30149:CDS | 15.0% |
| !! | AATAATTATTGCATAGTAAG+TGG | - | chr3.3:42607640-42607659 | MS.gene30149:CDS | 20.0% |
| !!! | CAACAAAACTTTGAAATATA+GGG | + | chr3.3:42607465-42607484 | None:intergenic | 20.0% |
| !!! | CTATATTTCAAAGTTTTGTT+GGG | - | chr3.3:42607464-42607483 | MS.gene30149:CDS | 20.0% |
| !!! | GATATTGTATTTTAAACTTG+GGG | - | chr3.3:42607714-42607733 | MS.gene30149:CDS | 20.0% |
| !!! | TATATTTCAAAGTTTTGTTG+GGG | - | chr3.3:42607465-42607484 | MS.gene30149:CDS | 20.0% |
| ! | AATGATCAATTGATTAGCAT+TGG | + | chr3.3:42607020-42607039 | None:intergenic | 25.0% |
| ! | ATGATCAATTGATTAGCATT+GGG | + | chr3.3:42607019-42607038 | None:intergenic | 25.0% |
| ! | CAATAATTATTGTTATACGG+CGG | + | chr3.3:42607631-42607650 | None:intergenic | 25.0% |
| !!! | CCAACAAAACTTTGAAATAT+AGG | + | chr3.3:42607466-42607485 | None:intergenic | 25.0% |
| !!! | CCTATATTTCAAAGTTTTGT+TGG | - | chr3.3:42607463-42607482 | MS.gene30149:CDS | 25.0% |
| TAGTGTTCTTTGTGAAAACT+TGG | + | chr3.3:42606922-42606941 | None:intergenic | 30.0% | |
| TTCAGTGGATATGAATATGT+GGG | - | chr3.3:42607195-42607214 | MS.gene30149:CDS | 30.0% | |
| ! | ATTGGGCCATTGATAATTAT+GGG | - | chr3.3:42607281-42607300 | MS.gene30149:CDS | 30.0% |
| !! | TGTATTTTAAACTTGGGGTT+AGG | - | chr3.3:42607719-42607738 | MS.gene30149:CDS | 30.0% |
| !!! | AACTAAAGCTTTTGAAGCTA+TGG | - | chr3.3:42607687-42607706 | MS.gene30149:CDS | 30.0% |
| ACAACATTGCTCATTTCGTT+GGG | - | chr3.3:42607157-42607176 | MS.gene30149:CDS | 35.0% | |
| ACCATGTTGAATCCAAACTA+GGG | + | chr3.3:42607108-42607127 | None:intergenic | 35.0% | |
| CATAATTATCAATGGCCCAA+TGG | + | chr3.3:42607282-42607301 | None:intergenic | 35.0% | |
| CATATCCACTGAACCTTTAA+TGG | + | chr3.3:42607188-42607207 | None:intergenic | 35.0% | |
| GTTACATCTTAGCTGATCTT+GGG | - | chr3.3:42607245-42607264 | MS.gene30149:CDS | 35.0% | |
| GTTCAGTGGATATGAATATG+TGG | - | chr3.3:42607194-42607213 | MS.gene30149:CDS | 35.0% | |
| GTTCTTTGTGAAAACTTGGT+GGG | + | chr3.3:42606918-42606937 | None:intergenic | 35.0% | |
| TAAGATGTAACCAATCCAAC+CGG | + | chr3.3:42607236-42607255 | None:intergenic | 35.0% | |
| TAGCCAACAATTTCATGCAT+GGG | - | chr3.3:42607510-42607529 | MS.gene30149:CDS | 35.0% | |
| TCGTGGAAAACAAGATCTAT+AGG | + | chr3.3:42607442-42607461 | None:intergenic | 35.0% | |
| TGTTCTTTGTGAAAACTTGG+TGG | + | chr3.3:42606919-42606938 | None:intergenic | 35.0% | |
| TTAGCCAACAATTTCATGCA+TGG | - | chr3.3:42607509-42607528 | MS.gene30149:CDS | 35.0% | |
| ! | CAATTGATTAGCATTGGGAT+TGG | + | chr3.3:42607014-42607033 | None:intergenic | 35.0% |
| ! | CATTGGGCCATTGATAATTA+TGG | - | chr3.3:42607280-42607299 | MS.gene30149:CDS | 35.0% |
| ! | TTCTTTGTGAAAACTTGGTG+GGG | + | chr3.3:42606917-42606936 | None:intergenic | 35.0% |
| ! | TTGGGCCATTGATAATTATG+GGG | - | chr3.3:42607282-42607301 | MS.gene30149:CDS | 35.0% |
| !! | AACTTTGAAATATAGGGTCG+TGG | + | chr3.3:42607459-42607478 | None:intergenic | 35.0% |
| !! | GCATCAGTTACCACTTTTAT+TGG | + | chr3.3:42607064-42607083 | None:intergenic | 35.0% |
| !! | TCAGTTACCACTTTTATTGG+CGG | + | chr3.3:42607061-42607080 | None:intergenic | 35.0% |
| AAACTAGGGTCATTGTCCAT+CGG | + | chr3.3:42607094-42607113 | None:intergenic | 40.0% | |
| AAGACACTTGTGTTGGACTA+AGG | + | chr3.3:42606885-42606904 | None:intergenic | 40.0% | |
| AATTATTGTTATACGGCGGC+CGG | + | chr3.3:42607627-42607646 | None:intergenic | 40.0% | |
| AATTTCATGCATGGGCTCAT+GGG | - | chr3.3:42607518-42607537 | MS.gene30149:CDS | 40.0% | |
| ACCCTAGTTTGGATTCAACA+TGG | - | chr3.3:42607104-42607123 | MS.gene30149:CDS | 40.0% | |
| AGAGTCCATTAAAGGTTCAG+TGG | - | chr3.3:42607180-42607199 | MS.gene30149:CDS | 40.0% | |
| AGTGGATATGAATATGTGGG+TGG | - | chr3.3:42607198-42607217 | MS.gene30149:CDS | 40.0% | |
| ATTGCATAGTAAGTGGAGTG+TGG | - | chr3.3:42607647-42607666 | MS.gene30149:CDS | 40.0% | |
| CACAACATTGCTCATTTCGT+TGG | - | chr3.3:42607156-42607175 | MS.gene30149:CDS | 40.0% | |
| CTCGTCCCCATAATTATCAA+TGG | + | chr3.3:42607290-42607309 | None:intergenic | 40.0% | |
| GACCATGTTGAATCCAAACT+AGG | + | chr3.3:42607109-42607128 | None:intergenic | 40.0% | |
| GGTTACATCTTAGCTGATCT+TGG | - | chr3.3:42607244-42607263 | MS.gene30149:CDS | 40.0% | |
| AACATGGTCTCATCGAGCAT+GGG | - | chr3.3:42607120-42607139 | MS.gene30149:CDS | 45.0% | |
| AATGGCCCAATGGTAAACTC+CGG | + | chr3.3:42607272-42607291 | None:intergenic | 45.0% | |
| ACCTTCTTCTGAGTGGATAG+AGG | - | chr3.3:42607759-42607778 | MS.gene30149:CDS | 45.0% | |
| AGCAATTGACGCAAATTCGG+TGG | + | chr3.3:42606949-42606968 | None:intergenic | 45.0% | |
| ATCTTAGCTGATCTTGGGTC+CGG | - | chr3.3:42607250-42607269 | MS.gene30149:CDS | 45.0% | |
| CAATTTCATGCATGGGCTCA+TGG | - | chr3.3:42607517-42607536 | MS.gene30149:CDS | 45.0% | |
| CGGAGCAATTGACGCAAATT+CGG | + | chr3.3:42606952-42606971 | None:intergenic | 45.0% | |
| CTTTCCAAGGCCATCATAAG+TGG | - | chr3.3:42607341-42607360 | MS.gene30149:CDS | 45.0% | |
| GACGAGTCAACACCATTAGT+TGG | - | chr3.3:42607304-42607323 | MS.gene30149:CDS | 45.0% | |
| GAGCCCATGCATGAAATTGT+TGG | + | chr3.3:42607516-42607535 | None:intergenic | 45.0% | |
| GAGTCAACACCATTAGTTGG+AGG | - | chr3.3:42607307-42607326 | MS.gene30149:CDS | 45.0% | |
| GATGGACAATGACCCTAGTT+TGG | - | chr3.3:42607093-42607112 | MS.gene30149:CDS | 45.0% | |
| GGCAAATTGACGCTTAGTGA+TGG | + | chr3.3:42607374-42607393 | None:intergenic | 45.0% | |
| TCCTCTATCCACTCAGAAGA+AGG | + | chr3.3:42607763-42607782 | None:intergenic | 45.0% | |
| TCGTTGGGAGAGTCCATTAA+AGG | - | chr3.3:42607172-42607191 | MS.gene30149:CDS | 45.0% | |
| TGGAGTGTGGAATGAGTTGT+TGG | - | chr3.3:42607660-42607679 | MS.gene30149:CDS | 45.0% | |
| TTAAACTTGGGGTTAGGCCT+AGG | - | chr3.3:42607725-42607744 | MS.gene30149:CDS | 45.0% | |
| ! | AAAACTTGGTGGGGAAAGCT+GGG | + | chr3.3:42606908-42606927 | None:intergenic | 45.0% |
| ! | AGCATTGGGATTGGACTTCA+GGG | + | chr3.3:42607005-42607024 | None:intergenic | 45.0% |
| ! | TAGCATTGGGATTGGACTTC+AGG | + | chr3.3:42607006-42607025 | None:intergenic | 45.0% |
| !! | TCGATTTGGCCTCCAACTAA+TGG | + | chr3.3:42607319-42607338 | None:intergenic | 45.0% |
| AACTGATGCTAACCGTCCGA+TGG | - | chr3.3:42607075-42607094 | MS.gene30149:CDS | 50.0% | |
| AAGGCCATCATAAGTGGCCA+TGG | - | chr3.3:42607347-42607366 | MS.gene30149:CDS | 50.0% | |
| AGCTGGGAAGACACTTGTGT+TGG | + | chr3.3:42606892-42606911 | None:intergenic | 50.0% | |
| ATGGCCACTTATGATGGCCT+TGG | + | chr3.3:42607348-42607367 | None:intergenic | 50.0% | |
| ATTGGCGGTCGTGCAAGTTT+CGG | + | chr3.3:42607046-42607065 | None:intergenic | 50.0% | |
| CAACATGGTCTCATCGAGCA+TGG | - | chr3.3:42607119-42607138 | MS.gene30149:CDS | 50.0% | |
| GAAGGTTCACTCCATGACCT+AGG | + | chr3.3:42607745-42607764 | None:intergenic | 50.0% | |
| GGAGTGAACCTTCTTCTGAG+TGG | - | chr3.3:42607752-42607771 | MS.gene30149:CDS | 50.0% | |
| GGCCAAATCGAAGCTTTCCA+AGG | - | chr3.3:42607328-42607347 | MS.gene30149:CDS | 50.0% | |
| GGCCTTGGAAAGCTTCGATT+TGG | + | chr3.3:42607333-42607352 | None:intergenic | 50.0% | |
| TAGGGTCATTGTCCATCGGA+CGG | + | chr3.3:42607090-42607109 | None:intergenic | 50.0% | |
| TATGTGGGTGGAGCCTATTG+TGG | - | chr3.3:42607210-42607229 | MS.gene30149:CDS | 50.0% | |
| TGACGCTTAGTGATGGTCCA+TGG | + | chr3.3:42607367-42607386 | None:intergenic | 50.0% | |
| TGCACGACCGCCAATAAAAG+TGG | - | chr3.3:42607051-42607070 | MS.gene30149:CDS | 50.0% | |
| TGCGAGTGCGTGCAAATTGT+TGG | + | chr3.3:42607395-42607414 | None:intergenic | 50.0% | |
| TGGGTCCGGAGTTTACCATT+GGG | - | chr3.3:42607264-42607283 | MS.gene30149:CDS | 50.0% | |
| TGGTCCATGGCCACTTATGA+TGG | + | chr3.3:42607354-42607373 | None:intergenic | 50.0% | |
| TTGGGTCCGGAGTTTACCAT+TGG | - | chr3.3:42607263-42607282 | MS.gene30149:CDS | 50.0% | |
| TTGTGTCACGGTCGCAACAT+GGG | - | chr3.3:42607593-42607612 | MS.gene30149:CDS | 50.0% | |
| ! | ACAGTGGAGTGCTTGTGTCA+CGG | - | chr3.3:42607581-42607600 | MS.gene30149:CDS | 50.0% |
| ! | GAAAACTTGGTGGGGAAAGC+TGG | + | chr3.3:42606909-42606928 | None:intergenic | 50.0% |
| !! | AGTATCAGTGGTAGTGGTGG+CGG | + | chr3.3:42606972-42606991 | None:intergenic | 50.0% |
| !! | TGGAGTATCAGTGGTAGTGG+TGG | + | chr3.3:42606975-42606994 | None:intergenic | 50.0% |
| CATCGAGCATGGGTGACAGT+TGG | - | chr3.3:42607130-42607149 | MS.gene30149:CDS | 55.0% | |
| CCAATCCAACCGGCCACAAT+AGG | + | chr3.3:42607226-42607245 | None:intergenic | 55.0% | |
| CCTATTGTGGCCGGTTGGAT+TGG | - | chr3.3:42607223-42607242 | MS.gene30149:CDS | 55.0% | |
| CTTGTGTCACGGTCGCAACA+TGG | - | chr3.3:42607592-42607611 | MS.gene30149:CDS | 55.0% | |
| GGGCTCATGGGACAAAGAGT+AGG | - | chr3.3:42607530-42607549 | MS.gene30149:CDS | 55.0% | |
| TATTGTTATACGGCGGCCGG+TGG | + | chr3.3:42607624-42607643 | None:intergenic | 55.0% | |
| TCTTGCAGAGCTACCACGAG+TGG | + | chr3.3:42607562-42607581 | None:intergenic | 55.0% | |
| TGTGTCACGGTCGCAACATG+GGG | - | chr3.3:42607594-42607613 | MS.gene30149:CDS | 55.0% | |
| TTGGGGTTAGGCCTAGGTCA+TGG | - | chr3.3:42607731-42607750 | MS.gene30149:CDS | 55.0% | |
| ! | GGCTGGAGTATCAGTGGTAG+TGG | + | chr3.3:42606978-42606997 | None:intergenic | 55.0% |
| ! | GTGGTAGCTCTGCAAGACAG+TGG | - | chr3.3:42607565-42607584 | MS.gene30149:CDS | 55.0% |
| !!! | AGTTTTGTTGGGGTGTGCGC+TGG | - | chr3.3:42607475-42607494 | MS.gene30149:CDS | 55.0% |
| GAGTAGGCTTCCACCACTCG+TGG | - | chr3.3:42607546-42607565 | MS.gene30149:CDS | 60.0% | |
| GGGATTGGACTTCAGGGCAG+TGG | + | chr3.3:42606999-42607018 | None:intergenic | 60.0% | |
| TGCAGAGCTACCACGAGTGG+TGG | + | chr3.3:42607559-42607578 | None:intergenic | 60.0% | |
| TGGAGCCTATTGTGGCCGGT+TGG | - | chr3.3:42607218-42607237 | MS.gene30149:CDS | 60.0% | |
| TGGGTGGAGCCTATTGTGGC+CGG | - | chr3.3:42607214-42607233 | MS.gene30149:CDS | 60.0% | |
| TTGGACTTCAGGGCAGTGGC+TGG | + | chr3.3:42606995-42607014 | None:intergenic | 60.0% | |
| TTTGCACGCACTCGCACGCA+TGG | - | chr3.3:42607399-42607418 | MS.gene30149:CDS | 60.0% | |
| ! | GGCAGTGGCTGGAGTATCAG+TGG | + | chr3.3:42606984-42607003 | None:intergenic | 60.0% |
| CGCAACATGGGGCACACCAC+CGG | - | chr3.3:42607605-42607624 | MS.gene30149:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 42606875 | 42607813 | 42606875 | ID=MS.gene30149 |
| chr3.3 | mRNA | 42606875 | 42607813 | 42606875 | ID=MS.gene30149.t1;Parent=MS.gene30149 |
| chr3.3 | exon | 42606875 | 42607813 | 42606875 | ID=MS.gene30149.t1.exon1;Parent=MS.gene30149.t1 |
| chr3.3 | CDS | 42606875 | 42607813 | 42606875 | ID=cds.MS.gene30149.t1;Parent=MS.gene30149.t1 |
| Gene Sequence |
| Protein sequence |
