AlfalfaGEDB | MS.gene30150

M. sativa cultivar XinJiangDaYe / MS.gene30150

KEGG: K20417
GO: -
PFAM: PF10520.12

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30150.t1	XP_003600055.1	86.2	311	32	1	1	300	1	311	2.40E-155	558.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30150.t1	Q9SZ42	56.8	294	105	2	26	297	27	320	8.9e-98	358.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30150.t1	G7IXK5	86.2	311	32	1	1	300	1	311	1.7e-155	558.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049232	MS.gene30150	0.814565	1.44E-51	-1.69E-46
MS.gene049356	MS.gene30150	0.82179	3.35E-53	-1.69E-46
MS.gene049369	MS.gene30150	0.82457	7.51E-54	-1.69E-46
MS.gene049396	MS.gene30150	0.871643	5.85E-67	-1.69E-46
MS.gene049656	MS.gene30150	0.80135	9.39E-49	-1.69E-46
MS.gene050323	MS.gene30150	0.816347	5.80E-52	-1.69E-46
MS.gene050380	MS.gene30150	-0.806839	6.77E-50	-1.69E-46
MS.gene050499	MS.gene30150	0.851062	1.13E-60	-1.69E-46
MS.gene050502	MS.gene30150	0.865252	6.76E-65	-1.69E-46
MS.gene050520	MS.gene30150	0.822116	2.81E-53	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene027119	MS.gene30150	PPI
MS.gene30149	MS.gene30150	PPI
MS.gene82707	MS.gene30150	PPI
MS.gene94356	MS.gene30150	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30150.t1	MTR_3g051220	88.103	311	26	1	1	300	1	311	0.0	568

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30150.t1	AT4G27030	63.910	266	86	1	32	297	65	320	5.03e-133	380
MS.gene30150.t1	AT2G22890	60.456	263	93	2	27	288	27	279	6.96e-120	345
MS.gene30150.t1	AT1G62190	56.250	272	108	3	26	294	25	288	9.56e-117	338

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Find 95 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGGGGTCATTAGTCT+TGG	0.476005	3.3:+42611598	None:intergenic
AACCGATGCTAATCGTCAAA+TGG	0.499165	3.3:-42612327	MS.gene30150:CDS
AACGTGGTCTCATCGAGCAT+GGG	0.579331	3.3:-42612282	MS.gene30150:CDS
AACTATTGTTATACGGTGGC+CGG	0.517622	3.3:+42611778	None:intergenic
AACTTTGAACTATAGGGTCA+TGG	0.588676	3.3:+42611946	None:intergenic
AAGGCCATCATAAGTGGCCA+TGG	0.554715	3.3:-42612055	MS.gene30150:CDS
AATAGTTATTGCATAGTAAG+TGG	0.480814	3.3:-42611762	MS.gene30150:CDS
AATGGACAATGACCCTAGTT+TGG	0.362427	3.3:-42612309	MS.gene30150:CDS
AATGGCCCAATGGTAAACTC+CGG	0.657151	3.3:+42612133	None:intergenic
AATTTCATGCATGGGCTCAT+GGG	0.536888	3.3:-42611884	MS.gene30150:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAATAACTATTGTTATACGG+TGG	+	chr3.3:42612334-42612353	None:intergenic	30.0%
CAATTGATTAGCATTAGGAT+TGG	+	chr3.3:42611717-42611736	None:intergenic	30.0%
CCAACAAAACTTTGAACTAT+AGG	+	chr3.3:42612169-42612188	None:intergenic	30.0%
GTTCAATCGATATGAATATG+TGG	-	chr3.3:42611897-42611916	MS.gene30150:CDS	30.0%
AACTTTGAACTATAGGGTCA+TGG	+	chr3.3:42612162-42612181	None:intergenic	35.0%
ACAACTTTGCTCATTTCGTT+GGG	-	chr3.3:42611860-42611879	MS.gene30150:CDS	35.0%
ATTGCATAGTAAGTGGAGTA+TGG	-	chr3.3:42612350-42612369	MS.gene30150:CDS	35.0%
CATAATTATCAATGGCCCAA+TGG	+	chr3.3:42611985-42612004	None:intergenic	35.0%
GTTACATCTTAGCTGATCTT+GGG	-	chr3.3:42611948-42611967	MS.gene30150:CDS	35.0%
TAGCCAACAATTTCATGCAT+GGG	-	chr3.3:42612213-42612232	MS.gene30150:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	42611611	42612516	42611611	ID=MS.gene30150
chr3.3	mRNA	42611611	42612516	42611611	ID=MS.gene30150.t1;Parent=MS.gene30150
chr3.3	exon	42611611	42612516	42611611	ID=MS.gene30150.t1.exon1;Parent=MS.gene30150.t1
chr3.3	CDS	42611611	42612516	42611611	ID=cds.MS.gene30150.t1;Parent=MS.gene30150.t1

Gene Sequence

>MS.gene30150

ATGTCTTCCCTAGTCCAACACAAGTGTCCTCCCAACTTTCACCACCAAGTTTGCACAAAGAATACCATCATTCACCGAATTCGCGTCACCACTGCCACGAAGTCCAATCCTAATGCTAATCAATTGATCATTGAGCCAAAATTTGCACGACCGCCAAAAAAAGTGGTAACCGATGCTAATCGTCAAATGGACAATGACCCTAGTTTGGAATCAACGTGGTCTCATCGAGCATGGGTGACTGTTGGATGCACAACTTTGCTCATTTCGTTGGGAGAGTCCATAAAGGGTTCAATCGATATGAATATGTGGGTTGAGCCCATTGTGGCAGGTTGGATTGGTTACATCTTAGCTGATCTTGGGTCCGGAGTTTACCATTGGGCCATTGATAATTATGGGGACGAGTCAACACCGTTAGTTGGAGCCCAACTTGAAGCTTCCCAAGGCCATCATAAGTGGCCATGGACCATTACTAAGCGTCAATTTGCCAACAATTTACATGCACTCGCACGTATGGTAACTTTTGTAGTGCTTCCTATCGATATTTTTTTCCATGACCCTATAGTTCAAAGTTTTGTTGGGGTGTGCGCTAGTTGTATTATGTTTAGCCAACAATTTCATGCATGGGCTCATGGGACAAAGAGTAGGCTTCCGCCTCTCGTGGTAGCTCTGCAAGACAGCGGGGTGCGTGTGTCACGATCGCATCATGGGGCGCACCACCGGCCACCGTATAACAATAGTTATTGCATAGTAAGTGGAGTATGGAATGAGTTTTTGGATAAAAGTAATGCTTTTGAAGTTATGGAAATGATATTGTATTTTAAACTTGGGGTTAGGCCTAGGTCTTGGAGTGAACCTTCTTCTGAGTGGATTGAGGAGATTAAGATTGCTTCGCAAAGCCAAGACTAA

Protein sequence

>MS.gene30150.t1

MSSLVQHKCPPNFHHQVCTKNTIIHRIRVTTATKSNPNANQLIIEPKFARPPKKVVTDANRQMDNDPSLESTWSHRAWVTVGCTTLLISLGESIKGSIDMNMWVEPIVAGWIGYILADLGSGVYHWAIDNYGDESTPLVGAQLEASQGHHKWPWTITKRQFANNLHALARMVTFVVLPIDIFFHDPIVQSFVGVCASCIMFSQQFHAWAHGTKSRLPPLVVALQDSGVRVSRSHHGAHHRPPYNNSYCIVSGVWNEFLDKSNAFEVMEMILYFKLGVRPRSWSEPSSEWIEEIKIASQSQD