Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82707.t1 | XP_003600055.1 | 90.7 | 311 | 29 | 0 | 1 | 311 | 1 | 311 | 1.90E-163 | 585.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82707.t1 | Q9SZ42 | 57.6 | 302 | 101 | 2 | 26 | 308 | 27 | 320 | 4.7e-102 | 372.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82707.t1 | G7IXK5 | 90.7 | 311 | 29 | 0 | 1 | 311 | 1 | 311 | 1.4e-163 | 585.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene82707 | 0.802221 | 6.22E-49 | -1.69E-46 |
| MS.gene049232 | MS.gene82707 | 0.861908 | 7.37E-64 | -1.69E-46 |
| MS.gene049396 | MS.gene82707 | 0.827204 | 1.78E-54 | -1.69E-46 |
| MS.gene049656 | MS.gene82707 | 0.815424 | 9.31E-52 | -1.69E-46 |
| MS.gene049657 | MS.gene82707 | 0.816265 | 6.05E-52 | -1.69E-46 |
| MS.gene05010 | MS.gene82707 | 0.818294 | 2.11E-52 | -1.69E-46 |
| MS.gene050170 | MS.gene82707 | 0.884516 | 1.79E-71 | -1.69E-46 |
| MS.gene050216 | MS.gene82707 | 0.823714 | 1.19E-53 | -1.69E-46 |
| MS.gene050323 | MS.gene82707 | 0.899893 | 1.26E-77 | -1.69E-46 |
| MS.gene050496 | MS.gene82707 | 0.881898 | 1.64E-70 | -1.69E-46 |
| MS.gene050502 | MS.gene82707 | 0.858901 | 5.99E-63 | -1.69E-46 |
| MS.gene050504 | MS.gene82707 | 0.812931 | 3.31E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene82707 | 0.811252 | 7.68E-51 | -1.69E-46 |
| MS.gene050612 | MS.gene82707 | 0.810194 | 1.30E-50 | -1.69E-46 |
| MS.gene050697 | MS.gene82707 | 0.878401 | 2.89E-69 | -1.69E-46 |
| MS.gene05080 | MS.gene82707 | 0.81205 | 5.15E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene82707 | 0.862872 | 3.72E-64 | -1.69E-46 |
| MS.gene050871 | MS.gene82707 | 0.864666 | 1.03E-64 | -1.69E-46 |
| MS.gene051268 | MS.gene82707 | 0.830093 | 3.57E-55 | -1.69E-46 |
| MS.gene051941 | MS.gene82707 | 0.80776 | 4.32E-50 | -1.69E-46 |
| MS.gene052066 | MS.gene82707 | 0.870063 | 1.94E-66 | -1.69E-46 |
| MS.gene052164 | MS.gene82707 | 0.807429 | 5.08E-50 | -1.69E-46 |
| MS.gene052482 | MS.gene82707 | 0.82883 | 7.23E-55 | -1.69E-46 |
| MS.gene052486 | MS.gene82707 | 0.835386 | 1.73E-56 | -1.69E-46 |
| MS.gene052503 | MS.gene82707 | 0.812526 | 4.06E-51 | -1.69E-46 |
| MS.gene052506 | MS.gene82707 | 0.806102 | 9.68E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene82707 | 0.810149 | 1.33E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene82707 | 0.815159 | 1.07E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene82707 | 0.808529 | 2.96E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene82707 | 0.816358 | 5.76E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene82707 | 0.805082 | 1.58E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene82707 | 0.814637 | 1.39E-51 | -1.69E-46 |
| MS.gene053574 | MS.gene82707 | 0.816174 | 6.33E-52 | -1.69E-46 |
| MS.gene053626 | MS.gene82707 | 0.837557 | 4.85E-57 | -1.69E-46 |
| MS.gene053633 | MS.gene82707 | 0.838024 | 3.68E-57 | -1.69E-46 |
| MS.gene053824 | MS.gene82707 | 0.831903 | 1.28E-55 | -1.69E-46 |
| MS.gene054003 | MS.gene82707 | 0.818413 | 1.99E-52 | -1.69E-46 |
| MS.gene054595 | MS.gene82707 | 0.809796 | 1.59E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene82707 | 0.834701 | 2.57E-56 | -1.69E-46 |
| MS.gene055502 | MS.gene82707 | 0.809315 | 2.01E-50 | -1.69E-46 |
| MS.gene055577 | MS.gene82707 | 0.807549 | 4.79E-50 | -1.69E-46 |
| MS.gene055748 | MS.gene82707 | 0.806024 | 1.01E-49 | -1.69E-46 |
| MS.gene055750 | MS.gene82707 | 0.8495 | 3.11E-60 | -1.69E-46 |
| MS.gene055751 | MS.gene82707 | 0.82146 | 3.99E-53 | -1.69E-46 |
| MS.gene055776 | MS.gene82707 | 0.811274 | 7.60E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene82707 | 0.864347 | 1.30E-64 | -1.69E-46 |
| MS.gene056046 | MS.gene82707 | 0.850445 | 1.69E-60 | -1.69E-46 |
| MS.gene056166 | MS.gene82707 | 0.824315 | 8.63E-54 | -1.69E-46 |
| MS.gene056387 | MS.gene82707 | 0.84708 | 1.45E-59 | -1.69E-46 |
| MS.gene056446 | MS.gene82707 | 0.828046 | 1.12E-54 | -1.69E-46 |
| MS.gene056504 | MS.gene82707 | 0.884487 | 1.84E-71 | -1.69E-46 |
| MS.gene056510 | MS.gene82707 | 0.844899 | 5.68E-59 | -1.69E-46 |
| MS.gene056878 | MS.gene82707 | 0.802756 | 4.82E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene82707 | 0.82229 | 2.56E-53 | -1.69E-46 |
| MS.gene057687 | MS.gene82707 | 0.81982 | 9.50E-53 | -1.69E-46 |
| MS.gene05787 | MS.gene82707 | 0.843702 | 1.19E-58 | -1.69E-46 |
| MS.gene058157 | MS.gene82707 | 0.830854 | 2.32E-55 | -1.69E-46 |
| MS.gene058171 | MS.gene82707 | -0.823892 | 1.08E-53 | -1.69E-46 |
| MS.gene058173 | MS.gene82707 | -0.830139 | 3.47E-55 | -1.69E-46 |
| MS.gene058175 | MS.gene82707 | -0.829356 | 5.39E-55 | -1.69E-46 |
| MS.gene058334 | MS.gene82707 | -0.811057 | 8.47E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene82707 | 0.825666 | 4.14E-54 | -1.69E-46 |
| MS.gene058382 | MS.gene82707 | -0.808419 | 3.13E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene82707 | 0.803456 | 3.46E-49 | -1.69E-46 |
| MS.gene059045 | MS.gene82707 | -0.811768 | 5.93E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene82707 | 0.820908 | 5.35E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene82707 | 0.824087 | 9.76E-54 | -1.69E-46 |
| MS.gene059667 | MS.gene82707 | 0.82698 | 2.01E-54 | -1.69E-46 |
| MS.gene059703 | MS.gene82707 | -0.815858 | 7.45E-52 | -1.69E-46 |
| MS.gene059837 | MS.gene82707 | 0.833519 | 5.09E-56 | -1.69E-46 |
| MS.gene059865 | MS.gene82707 | 0.864542 | 1.13E-64 | -1.69E-46 |
| MS.gene05996 | MS.gene82707 | 0.811359 | 7.28E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene82707 | 0.860789 | 1.62E-63 | -1.69E-46 |
| MS.gene060593 | MS.gene82707 | -0.815374 | 9.55E-52 | -1.69E-46 |
| MS.gene060595 | MS.gene82707 | -0.843544 | 1.31E-58 | -1.69E-46 |
| MS.gene060703 | MS.gene82707 | 0.807993 | 3.85E-50 | -1.69E-46 |
| MS.gene060778 | MS.gene82707 | 0.890818 | 6.98E-74 | -1.69E-46 |
| MS.gene06077 | MS.gene82707 | 0.824931 | 6.18E-54 | -1.69E-46 |
| MS.gene06097 | MS.gene82707 | 0.835126 | 2.01E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene82707 | 0.88424 | 2.27E-71 | -1.69E-46 |
| MS.gene061044 | MS.gene82707 | -0.809313 | 2.01E-50 | -1.69E-46 |
| MS.gene061218 | MS.gene82707 | 0.820572 | 6.39E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene82707 | MS.gene30150 | PPI |
| MS.gene94356 | MS.gene82707 | PPI |
| MS.gene30149 | MS.gene82707 | PPI |
| MS.gene027119 | MS.gene82707 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82707.t1 | MTR_3g051220 | 96.785 | 311 | 10 | 0 | 1 | 311 | 1 | 311 | 0.0 | 627 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82707.t1 | AT4G27030 | 60.526 | 304 | 89 | 2 | 26 | 308 | 27 | 320 | 1.20e-139 | 397 |
| MS.gene82707.t1 | AT1G62190 | 56.071 | 280 | 107 | 2 | 26 | 305 | 25 | 288 | 1.74e-120 | 348 |
| MS.gene82707.t1 | AT2G22890 | 60.547 | 256 | 90 | 2 | 45 | 299 | 34 | 279 | 1.49e-115 | 335 |
Find 106 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATATCCATCGAACCTTTAA+TGG | 0.128810 | 3.1:+38180432 | None:intergenic |
| CATTGGGCCATTGATAATTA+TGG | 0.152142 | 3.1:-38180337 | MS.gene82707:CDS |
| GGCCTTGAAAAGCTTCAATT+TGG | 0.208933 | 3.1:+38180287 | None:intergenic |
| TAGCATTGGGCTTGGACTTC+AGG | 0.233568 | 3.1:+38180614 | None:intergenic |
| TCGTTGGGAGAGTCCATTAA+AGG | 0.296680 | 3.1:-38180445 | MS.gene82707:CDS |
| GGTTACATCTTAGCTGATCT+TGG | 0.300492 | 3.1:-38180373 | MS.gene82707:CDS |
| CCAACAAAACTTTGAACAAT+AGG | 0.307472 | 3.1:+38180154 | None:intergenic |
| ATGCAATAATTATTGTTATA+CGG | 0.313977 | 3.1:+38179986 | None:intergenic |
| ATTGGCGGTCGTGCAAGTTT+CGG | 0.325462 | 3.1:+38180574 | None:intergenic |
| AATGGACAATGACCCTAGTT+TGG | 0.384966 | 3.1:-38180524 | MS.gene82707:CDS |
| ATGATATTGTATTTCAAACT+TGG | 0.395567 | 3.1:-38179905 | MS.gene82707:CDS |
| GTTACATCTTAGCTGATCTT+GGG | 0.396076 | 3.1:-38180372 | MS.gene82707:CDS |
| CCAATCCAACCTGCCACAAT+AGG | 0.396466 | 3.1:+38180394 | None:intergenic |
| GAAAGAAGGGATCTTAGGTT+TGG | 0.398872 | 3.1:+38179813 | None:intergenic |
| GCCTTGAAAAGCTTCAATTT+GGG | 0.400829 | 3.1:+38180288 | None:intergenic |
| CACAACATTGCTCATTTCGT+TGG | 0.401615 | 3.1:-38180461 | MS.gene82707:CDS |
| CAATTGATTAGCATTGGGCT+TGG | 0.402702 | 3.1:+38180606 | None:intergenic |
| CAATTTCATGCATGGGCTCA+TGG | 0.404441 | 3.1:-38180100 | MS.gene82707:CDS |
| CGGAGCAATTGACGCGAATT+CGG | 0.404513 | 3.1:+38180668 | None:intergenic |
| CCTATTGTGGCAGGTTGGAT+TGG | 0.412756 | 3.1:-38180394 | MS.gene82707:CDS |
| CAATTTGGGCACCAACCAAT+GGG | 0.415017 | 3.1:+38180302 | None:intergenic |
| TGTATTTCAAACTTGGGGTC+AGG | 0.426129 | 3.1:-38179898 | MS.gene82707:CDS |
| ATGATCAATTGATTAGCATT+GGG | 0.426195 | 3.1:+38180601 | None:intergenic |
| TGGTAGCTCTGCAAGACAGC+GGG | 0.430058 | 3.1:-38180051 | MS.gene82707:CDS |
| TGATATTGTATTTCAAACTT+GGG | 0.432835 | 3.1:-38179904 | MS.gene82707:CDS |
| TTGGGTCCGGAGTTTACCAT+TGG | 0.438101 | 3.1:-38180354 | MS.gene82707:CDS |
| AATGATCAATTGATTAGCAT+TGG | 0.438397 | 3.1:+38180600 | None:intergenic |
| TCATGGAAAAGAAGATTGAT+AGG | 0.446735 | 3.1:+38180178 | None:intergenic |
| AATAATTATTGCATAGTAAG+TGG | 0.447132 | 3.1:-38179977 | MS.gene82707:CDS |
| TCAAACTTGGGGTCAGGCCT+AGG | 0.448580 | 3.1:-38179892 | MS.gene82707:CDS |
| ATCTTAGCTGATCTTGGGTC+CGG | 0.450717 | 3.1:-38180367 | MS.gene82707:CDS |
| CAACAAAACTTTGAACAATA+GGG | 0.456010 | 3.1:+38180155 | None:intergenic |
| GCCATTGATAATTATGGAGA+TGG | 0.463376 | 3.1:-38180331 | MS.gene82707:CDS |
| GGGCTTGGACTTCAGGGCAG+TGG | 0.471613 | 3.1:+38180621 | None:intergenic |
| AGCATTGGGCTTGGACTTCA+GGG | 0.478698 | 3.1:+38180615 | None:intergenic |
| GAAAACTTGGTGGTGAAAGC+TGG | 0.480653 | 3.1:+38180711 | None:intergenic |
| CTTGTGTCACGGTCACAACA+TGG | 0.482681 | 3.1:-38180025 | MS.gene82707:CDS |
| TCCTCTATCCACTCAGAAGA+AGG | 0.484874 | 3.1:+38179857 | None:intergenic |
| TTGGCTAAACATAATACAAC+CGG | 0.486952 | 3.1:+38180123 | None:intergenic |
| ACAACATTGCTCATTTCGTT+GGG | 0.487853 | 3.1:-38180460 | MS.gene82707:CDS |
| TGCGAGTGAGTGCAAATTGT+TGG | 0.491754 | 3.1:+38180225 | None:intergenic |
| GACCATGTTGATTCCAAACT+AGG | 0.495633 | 3.1:+38180511 | None:intergenic |
| TAGCCAACAATTTCATGCAT+GGG | 0.501565 | 3.1:-38180107 | MS.gene82707:CDS |
| CACAACATGGGGCACACCAC+CGG | 0.502734 | 3.1:-38180012 | MS.gene82707:CDS |
| TCAATTTGGGCACCAACCAA+TGG | 0.503580 | 3.1:+38180301 | None:intergenic |
| GGCAAATTGACGCTTAGTAA+TGG | 0.504859 | 3.1:+38180246 | None:intergenic |
| AAGACACTTGTGTTGGACTA+AGG | 0.508363 | 3.1:+38180735 | None:intergenic |
| GAGCCCATGCATGAAATTGT+TGG | 0.514086 | 3.1:+38180104 | None:intergenic |
| TGGTCCACGGCCACTTATGA+TGG | 0.515734 | 3.1:+38180266 | None:intergenic |
| TGGAGCCTATTGTGGCAGGT+TGG | 0.518996 | 3.1:-38180399 | MS.gene82707:CDS |
| TGGAGATGGGTCAACCCCAT+TGG | 0.519914 | 3.1:-38180317 | MS.gene82707:CDS |
| CCCATCTCCATAATTATCAA+TGG | 0.520986 | 3.1:+38180330 | None:intergenic |
| CAACATGGTCTCATCGAGCA+TGG | 0.522244 | 3.1:-38180498 | MS.gene82707:CDS |
| TGGGTCCGGAGTTTACCATT+GGG | 0.524693 | 3.1:-38180353 | MS.gene82707:CDS |
| TTGGGGTCAGGCCTAGGTCA+TGG | 0.530441 | 3.1:-38179886 | MS.gene82707:CDS |
| GCCTTCTTCTGAGTGGATAG+AGG | 0.532572 | 3.1:-38179858 | MS.gene82707:CDS |
| AAACTAGGGTCATTGTCCAT+TGG | 0.532832 | 3.1:+38180526 | None:intergenic |
| TTTGCACTCACTCGCACGTA+TGG | 0.534993 | 3.1:-38180218 | MS.gene82707:CDS |
| TGCACGACCGCCAATAAAAG+TGG | 0.536589 | 3.1:-38180566 | MS.gene82707:CDS |
| AATTTCATGCATGGGCTCAT+GGG | 0.536888 | 3.1:-38180099 | MS.gene82707:CDS |
| AATTATTGTTATACGGCGGC+CGG | 0.539022 | 3.1:+38179993 | None:intergenic |
| TAGTGTTCTTTGTGAAAACT+TGG | 0.545737 | 3.1:+38180698 | None:intergenic |
| CATAATTATCAATGGCCCAA+TGG | 0.547046 | 3.1:+38180338 | None:intergenic |
| AGTATCAGTGGTAGTGGTGG+CGG | 0.547623 | 3.1:+38180648 | None:intergenic |
| GTTCGATGGATATGAATATG+TGG | 0.552737 | 3.1:-38180423 | MS.gene82707:CDS |
| AAGGCCATCATAAGTGGCCG+TGG | 0.552800 | 3.1:-38180270 | MS.gene82707:CDS |
| CATCGAGCATGGGTGACAGT+TGG | 0.553692 | 3.1:-38180487 | MS.gene82707:CDS |
| TGGGTGGAGCCTATTGTGGC+AGG | 0.553913 | 3.1:-38180403 | MS.gene82707:CDS |
| TTAGCCAACAATTTCATGCA+TGG | 0.554178 | 3.1:-38180108 | MS.gene82707:CDS |
| TATTGTTATACGGCGGCCGG+TGG | 0.555619 | 3.1:+38179996 | None:intergenic |
| AGCTGGGAAGACACTTGTGT+TGG | 0.564987 | 3.1:+38180728 | None:intergenic |
| AACTGATGCTAACCGTCCAA+TGG | 0.569213 | 3.1:-38180542 | MS.gene82707:CDS |
| GATATTGTATTTCAAACTTG+GGG | 0.574716 | 3.1:-38179903 | MS.gene82707:CDS |
| GATGGGTCAACCCCATTGGT+TGG | 0.574822 | 3.1:-38180313 | MS.gene82707:CDS |
| AACATGGTCTCATCGAGCAT+GGG | 0.579331 | 3.1:-38180497 | MS.gene82707:CDS |
| GGCATGAGTATCAGTGGTAG+TGG | 0.581600 | 3.1:+38180642 | None:intergenic |
| ACAGCGGGGTGCTTGTGTCA+CGG | 0.582100 | 3.1:-38180036 | MS.gene82707:CDS |
| TGTTCTTTGTGAAAACTTGG+TGG | 0.585410 | 3.1:+38180701 | None:intergenic |
| AGAGTCCATTAAAGGTTCGA+TGG | 0.586164 | 3.1:-38180437 | MS.gene82707:CDS |
| AACTTTGAACAATAGGGTCA+TGG | 0.591159 | 3.1:+38180161 | None:intergenic |
| AAAACTTGGTGGTGAAAGCT+GGG | 0.597144 | 3.1:+38180712 | None:intergenic |
| TGCAGAGCTACCACGAGCGG+TGG | 0.597300 | 3.1:+38180061 | None:intergenic |
| ACCATGTTGATTCCAAACTA+GGG | 0.597315 | 3.1:+38180512 | None:intergenic |
| TTGTGTCACGGTCACAACAT+GGG | 0.597584 | 3.1:-38180024 | MS.gene82707:CDS |
| GGGCTCATGGGACAAAGAGT+AGG | 0.597966 | 3.1:-38180087 | MS.gene82707:CDS |
| TTCGATGGATATGAATATGT+GGG | 0.600883 | 3.1:-38180422 | MS.gene82707:CDS |
| AGCAATTGACGCGAATTCGG+TGG | 0.602094 | 3.1:+38180671 | None:intergenic |
| GAGTAGGCTTCCACCGCTCG+TGG | 0.605399 | 3.1:-38180071 | MS.gene82707:CDS |
| CCATTGATAATTATGGAGAT+GGG | 0.605803 | 3.1:-38180330 | MS.gene82707:CDS |
| GGCAGTGGCATGAGTATCAG+TGG | 0.608079 | 3.1:+38180636 | None:intergenic |
| GAAGGCTCACTCCATGACCT+AGG | 0.616374 | 3.1:+38179875 | None:intergenic |
| TCTTGCAGAGCTACCACGAG+CGG | 0.618762 | 3.1:+38180058 | None:intergenic |
| ATTGCATAGTAAGTGGAGTG+TGG | 0.622359 | 3.1:-38179970 | MS.gene82707:CDS |
| TGTGTCACGGTCACAACATG+GGG | 0.623504 | 3.1:-38180023 | MS.gene82707:CDS |
| TAGGGTCATTGTCCATTGGA+CGG | 0.625760 | 3.1:+38180530 | None:intergenic |
| GTGGTAGCTCTGCAAGACAG+CGG | 0.633692 | 3.1:-38180052 | MS.gene82707:CDS |
| AATGGCCCAATGGTAAACTC+CGG | 0.657151 | 3.1:+38180348 | None:intergenic |
| GGAGTGAGCCTTCTTCTGAG+TGG | 0.666960 | 3.1:-38179865 | MS.gene82707:CDS |
| TGACGCTTAGTAATGGTCCA+CGG | 0.667756 | 3.1:+38180253 | None:intergenic |
| ACCCTAGTTTGGAATCAACA+TGG | 0.676174 | 3.1:-38180513 | MS.gene82707:CDS |
| ATGAGTATCAGTGGTAGTGG+TGG | 0.676996 | 3.1:+38180645 | None:intergenic |
| GGTAGCTCTGCAAGACAGCG+GGG | 0.678989 | 3.1:-38180050 | MS.gene82707:CDS |
| CAATAATTATTGTTATACGG+CGG | 0.684485 | 3.1:+38179989 | None:intergenic |
| TATGTGGGTGGAGCCTATTG+TGG | 0.686763 | 3.1:-38180407 | MS.gene82707:CDS |
| AATTTGGGCACCAACCAATG+GGG | 0.702858 | 3.1:+38180303 | None:intergenic |
| GATGGATATGAATATGTGGG+TGG | 0.713715 | 3.1:-38180419 | MS.gene82707:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGCAATAATTATTGTTATA+CGG | + | chr3.1:38180585-38180604 | None:intergenic | 15.0% |
| !! | AATAATTATTGCATAGTAAG+TGG | - | chr3.1:38180591-38180610 | MS.gene82707:CDS | 20.0% |
| !! | ATGATATTGTATTTCAAACT+TGG | - | chr3.1:38180663-38180682 | MS.gene82707:CDS | 20.0% |
| !! | TGATATTGTATTTCAAACTT+GGG | - | chr3.1:38180664-38180683 | MS.gene82707:CDS | 20.0% |
| ! | AATGATCAATTGATTAGCAT+TGG | + | chr3.1:38179971-38179990 | None:intergenic | 25.0% |
| ! | ATGATCAATTGATTAGCATT+GGG | + | chr3.1:38179970-38179989 | None:intergenic | 25.0% |
| ! | CAACAAAACTTTGAACAATA+GGG | + | chr3.1:38180416-38180435 | None:intergenic | 25.0% |
| ! | CAATAATTATTGTTATACGG+CGG | + | chr3.1:38180582-38180601 | None:intergenic | 25.0% |
| ! | GATATTGTATTTCAAACTTG+GGG | - | chr3.1:38180665-38180684 | MS.gene82707:CDS | 25.0% |
| !!! | CTATTGTTCAAAGTTTTGTT+GGG | - | chr3.1:38180415-38180434 | MS.gene82707:CDS | 25.0% |
| !!! | TATTGTTCAAAGTTTTGTTG+GGG | - | chr3.1:38180416-38180435 | MS.gene82707:CDS | 25.0% |
| CCAACAAAACTTTGAACAAT+AGG | + | chr3.1:38180417-38180436 | None:intergenic | 30.0% | |
| TAGTGTTCTTTGTGAAAACT+TGG | + | chr3.1:38179873-38179892 | None:intergenic | 30.0% | |
| TCATGGAAAAGAAGATTGAT+AGG | + | chr3.1:38180393-38180412 | None:intergenic | 30.0% | |
| TTCGATGGATATGAATATGT+GGG | - | chr3.1:38180146-38180165 | MS.gene82707:CDS | 30.0% | |
| TTGGCTAAACATAATACAAC+CGG | + | chr3.1:38180448-38180467 | None:intergenic | 30.0% | |
| ! | CCATTGATAATTATGGAGAT+GGG | - | chr3.1:38180238-38180257 | MS.gene82707:CDS | 30.0% |
| !! | CCTATTGTTCAAAGTTTTGT+TGG | - | chr3.1:38180414-38180433 | MS.gene82707:CDS | 30.0% |
| !!! | AACTAAAGCTTTTGAAGCTA+TGG | - | chr3.1:38180638-38180657 | MS.gene82707:CDS | 30.0% |
| AACTTTGAACAATAGGGTCA+TGG | + | chr3.1:38180410-38180429 | None:intergenic | 35.0% | |
| ACAACATTGCTCATTTCGTT+GGG | - | chr3.1:38180108-38180127 | MS.gene82707:CDS | 35.0% | |
| CATAATTATCAATGGCCCAA+TGG | + | chr3.1:38180233-38180252 | None:intergenic | 35.0% | |
| CATATCCATCGAACCTTTAA+TGG | + | chr3.1:38180139-38180158 | None:intergenic | 35.0% | |
| CCCATCTCCATAATTATCAA+TGG | + | chr3.1:38180241-38180260 | None:intergenic | 35.0% | |
| GCCTTGAAAAGCTTCAATTT+GGG | + | chr3.1:38180283-38180302 | None:intergenic | 35.0% | |
| GTTACATCTTAGCTGATCTT+GGG | - | chr3.1:38180196-38180215 | MS.gene82707:CDS | 35.0% | |
| GTTCGATGGATATGAATATG+TGG | - | chr3.1:38180145-38180164 | MS.gene82707:CDS | 35.0% | |
| TAGCCAACAATTTCATGCAT+GGG | - | chr3.1:38180461-38180480 | MS.gene82707:CDS | 35.0% | |
| TGTTCTTTGTGAAAACTTGG+TGG | + | chr3.1:38179870-38179889 | None:intergenic | 35.0% | |
| TTAGCCAACAATTTCATGCA+TGG | - | chr3.1:38180460-38180479 | MS.gene82707:CDS | 35.0% | |
| ! | CATTGGGCCATTGATAATTA+TGG | - | chr3.1:38180231-38180250 | MS.gene82707:CDS | 35.0% |
| ! | GCCATTGATAATTATGGAGA+TGG | - | chr3.1:38180237-38180256 | MS.gene82707:CDS | 35.0% |
| !! | ACCATGTTGATTCCAAACTA+GGG | + | chr3.1:38180059-38180078 | None:intergenic | 35.0% |
| !! | GCATCAGTTACCACTTTTAT+TGG | + | chr3.1:38180015-38180034 | None:intergenic | 35.0% |
| !! | TCAGTTACCACTTTTATTGG+CGG | + | chr3.1:38180012-38180031 | None:intergenic | 35.0% |
| AAACTAGGGTCATTGTCCAT+TGG | + | chr3.1:38180045-38180064 | None:intergenic | 40.0% | |
| AAGACACTTGTGTTGGACTA+AGG | + | chr3.1:38179836-38179855 | None:intergenic | 40.0% | |
| AATGGACAATGACCCTAGTT+TGG | - | chr3.1:38180044-38180063 | MS.gene82707:CDS | 40.0% | |
| AATTATTGTTATACGGCGGC+CGG | + | chr3.1:38180578-38180597 | None:intergenic | 40.0% | |
| AATTTCATGCATGGGCTCAT+GGG | - | chr3.1:38180469-38180488 | MS.gene82707:CDS | 40.0% | |
| AGAGTCCATTAAAGGTTCGA+TGG | - | chr3.1:38180131-38180150 | MS.gene82707:CDS | 40.0% | |
| ATTGCATAGTAAGTGGAGTG+TGG | - | chr3.1:38180598-38180617 | MS.gene82707:CDS | 40.0% | |
| CACAACATTGCTCATTTCGT+TGG | - | chr3.1:38180107-38180126 | MS.gene82707:CDS | 40.0% | |
| GATGGATATGAATATGTGGG+TGG | - | chr3.1:38180149-38180168 | MS.gene82707:CDS | 40.0% | |
| GGCAAATTGACGCTTAGTAA+TGG | + | chr3.1:38180325-38180344 | None:intergenic | 40.0% | |
| GGCCTTGAAAAGCTTCAATT+TGG | + | chr3.1:38180284-38180303 | None:intergenic | 40.0% | |
| GGTTACATCTTAGCTGATCT+TGG | - | chr3.1:38180195-38180214 | MS.gene82707:CDS | 40.0% | |
| TGTATTTCAAACTTGGGGTC+AGG | - | chr3.1:38180670-38180689 | MS.gene82707:CDS | 40.0% | |
| ! | ACCCTAGTTTGGAATCAACA+TGG | - | chr3.1:38180055-38180074 | MS.gene82707:CDS | 40.0% |
| ! | CAATTGATTAGCATTGGGCT+TGG | + | chr3.1:38179965-38179984 | None:intergenic | 40.0% |
| ! | GCCCAAATTGAAGCTTTTCA+AGG | - | chr3.1:38180279-38180298 | MS.gene82707:CDS | 40.0% |
| ! | TGGAGTGTGGAATGAGTTTT+TGG | - | chr3.1:38180611-38180630 | MS.gene82707:CDS | 40.0% |
| !! | AAAACTTGGTGGTGAAAGCT+GGG | + | chr3.1:38179859-38179878 | None:intergenic | 40.0% |
| !! | CTTTTCAAGGCCATCATAAG+TGG | - | chr3.1:38180292-38180311 | MS.gene82707:CDS | 40.0% |
| !! | GACCATGTTGATTCCAAACT+AGG | + | chr3.1:38180060-38180079 | None:intergenic | 40.0% |
| AACATGGTCTCATCGAGCAT+GGG | - | chr3.1:38180071-38180090 | MS.gene82707:CDS | 45.0% | |
| AACTGATGCTAACCGTCCAA+TGG | - | chr3.1:38180026-38180045 | MS.gene82707:CDS | 45.0% | |
| AATGGCCCAATGGTAAACTC+CGG | + | chr3.1:38180223-38180242 | None:intergenic | 45.0% | |
| AATTTGGGCACCAACCAATG+GGG | + | chr3.1:38180268-38180287 | None:intergenic | 45.0% | |
| ATCTTAGCTGATCTTGGGTC+CGG | - | chr3.1:38180201-38180220 | MS.gene82707:CDS | 45.0% | |
| CAATTTCATGCATGGGCTCA+TGG | - | chr3.1:38180468-38180487 | MS.gene82707:CDS | 45.0% | |
| CAATTTGGGCACCAACCAAT+GGG | + | chr3.1:38180269-38180288 | None:intergenic | 45.0% | |
| GAGCCCATGCATGAAATTGT+TGG | + | chr3.1:38180467-38180486 | None:intergenic | 45.0% | |
| TAGGGTCATTGTCCATTGGA+CGG | + | chr3.1:38180041-38180060 | None:intergenic | 45.0% | |
| TCAATTTGGGCACCAACCAA+TGG | + | chr3.1:38180270-38180289 | None:intergenic | 45.0% | |
| TCCTCTATCCACTCAGAAGA+AGG | + | chr3.1:38180714-38180733 | None:intergenic | 45.0% | |
| TCGTTGGGAGAGTCCATTAA+AGG | - | chr3.1:38180123-38180142 | MS.gene82707:CDS | 45.0% | |
| TGACGCTTAGTAATGGTCCA+CGG | + | chr3.1:38180318-38180337 | None:intergenic | 45.0% | |
| TGCGAGTGAGTGCAAATTGT+TGG | + | chr3.1:38180346-38180365 | None:intergenic | 45.0% | |
| TTGTGTCACGGTCACAACAT+GGG | - | chr3.1:38180544-38180563 | MS.gene82707:CDS | 45.0% | |
| !! | ATGAGTATCAGTGGTAGTGG+TGG | + | chr3.1:38179926-38179945 | None:intergenic | 45.0% |
| !! | GAAAACTTGGTGGTGAAAGC+TGG | + | chr3.1:38179860-38179879 | None:intergenic | 45.0% |
| AGCAATTGACGCGAATTCGG+TGG | + | chr3.1:38179900-38179919 | None:intergenic | 50.0% | |
| AGCTGGGAAGACACTTGTGT+TGG | + | chr3.1:38179843-38179862 | None:intergenic | 50.0% | |
| ATTGGCGGTCGTGCAAGTTT+CGG | + | chr3.1:38179997-38180016 | None:intergenic | 50.0% | |
| CAACATGGTCTCATCGAGCA+TGG | - | chr3.1:38180070-38180089 | MS.gene82707:CDS | 50.0% | |
| CCAATCCAACCTGCCACAAT+AGG | + | chr3.1:38180177-38180196 | None:intergenic | 50.0% | |
| CGGAGCAATTGACGCGAATT+CGG | + | chr3.1:38179903-38179922 | None:intergenic | 50.0% | |
| CTTGTGTCACGGTCACAACA+TGG | - | chr3.1:38180543-38180562 | MS.gene82707:CDS | 50.0% | |
| GCCTTCTTCTGAGTGGATAG+AGG | - | chr3.1:38180710-38180729 | MS.gene82707:CDS | 50.0% | |
| GGCATGAGTATCAGTGGTAG+TGG | + | chr3.1:38179929-38179948 | None:intergenic | 50.0% | |
| TATGTGGGTGGAGCCTATTG+TGG | - | chr3.1:38180161-38180180 | MS.gene82707:CDS | 50.0% | |
| TGCACGACCGCCAATAAAAG+TGG | - | chr3.1:38180002-38180021 | MS.gene82707:CDS | 50.0% | |
| TGGGTCCGGAGTTTACCATT+GGG | - | chr3.1:38180215-38180234 | MS.gene82707:CDS | 50.0% | |
| TGTGTCACGGTCACAACATG+GGG | - | chr3.1:38180545-38180564 | MS.gene82707:CDS | 50.0% | |
| TTGGGTCCGGAGTTTACCAT+TGG | - | chr3.1:38180214-38180233 | MS.gene82707:CDS | 50.0% | |
| TTTGCACTCACTCGCACGTA+TGG | - | chr3.1:38180350-38180369 | MS.gene82707:CDS | 50.0% | |
| ! | AGCATTGGGCTTGGACTTCA+GGG | + | chr3.1:38179956-38179975 | None:intergenic | 50.0% |
| ! | CCTATTGTGGCAGGTTGGAT+TGG | - | chr3.1:38180174-38180193 | MS.gene82707:CDS | 50.0% |
| ! | TAGCATTGGGCTTGGACTTC+AGG | + | chr3.1:38179957-38179976 | None:intergenic | 50.0% |
| !! | AGTATCAGTGGTAGTGGTGG+CGG | + | chr3.1:38179923-38179942 | None:intergenic | 50.0% |
| !!! | AGTTTTGTTGGGGTGTGTGC+CGG | - | chr3.1:38180426-38180445 | MS.gene82707:CDS | 50.0% |
| AAGGCCATCATAAGTGGCCG+TGG | - | chr3.1:38180298-38180317 | MS.gene82707:CDS | 55.0% | |
| CATCGAGCATGGGTGACAGT+TGG | - | chr3.1:38180081-38180100 | MS.gene82707:CDS | 55.0% | |
| GAAGGCTCACTCCATGACCT+AGG | + | chr3.1:38180696-38180715 | None:intergenic | 55.0% | |
| GATGGGTCAACCCCATTGGT+TGG | - | chr3.1:38180255-38180274 | MS.gene82707:CDS | 55.0% | |
| GGAGTGAGCCTTCTTCTGAG+TGG | - | chr3.1:38180703-38180722 | MS.gene82707:CDS | 55.0% | |
| GGCAGTGGCATGAGTATCAG+TGG | + | chr3.1:38179935-38179954 | None:intergenic | 55.0% | |
| GGGCTCATGGGACAAAGAGT+AGG | - | chr3.1:38180481-38180500 | MS.gene82707:CDS | 55.0% | |
| TATTGTTATACGGCGGCCGG+TGG | + | chr3.1:38180575-38180594 | None:intergenic | 55.0% | |
| TCAAACTTGGGGTCAGGCCT+AGG | - | chr3.1:38180676-38180695 | MS.gene82707:CDS | 55.0% | |
| TCTTGCAGAGCTACCACGAG+CGG | + | chr3.1:38180513-38180532 | None:intergenic | 55.0% | |
| TGGAGATGGGTCAACCCCAT+TGG | - | chr3.1:38180251-38180270 | MS.gene82707:CDS | 55.0% | |
| TGGAGCCTATTGTGGCAGGT+TGG | - | chr3.1:38180169-38180188 | MS.gene82707:CDS | 55.0% | |
| TGGTCCACGGCCACTTATGA+TGG | + | chr3.1:38180305-38180324 | None:intergenic | 55.0% | |
| ! | GTGGTAGCTCTGCAAGACAG+CGG | - | chr3.1:38180516-38180535 | MS.gene82707:CDS | 55.0% |
| ! | TGGTAGCTCTGCAAGACAGC+GGG | - | chr3.1:38180517-38180536 | MS.gene82707:CDS | 55.0% |
| CACAACATGGGGCACACCAC+CGG | - | chr3.1:38180556-38180575 | MS.gene82707:CDS | 60.0% | |
| TGGGTGGAGCCTATTGTGGC+AGG | - | chr3.1:38180165-38180184 | MS.gene82707:CDS | 60.0% | |
| TTGGGGTCAGGCCTAGGTCA+TGG | - | chr3.1:38180682-38180701 | MS.gene82707:CDS | 60.0% | |
| ! | ACAGCGGGGTGCTTGTGTCA+CGG | - | chr3.1:38180532-38180551 | MS.gene82707:CDS | 60.0% |
| ! | GGTAGCTCTGCAAGACAGCG+GGG | - | chr3.1:38180518-38180537 | MS.gene82707:CDS | 60.0% |
| GAGTAGGCTTCCACCGCTCG+TGG | - | chr3.1:38180497-38180516 | MS.gene82707:CDS | 65.0% | |
| GGGCTTGGACTTCAGGGCAG+TGG | + | chr3.1:38179950-38179969 | None:intergenic | 65.0% | |
| TGCAGAGCTACCACGAGCGG+TGG | + | chr3.1:38180510-38180529 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 38179826 | 38180764 | 38179826 | ID=MS.gene82707 |
| chr3.1 | mRNA | 38179826 | 38180764 | 38179826 | ID=MS.gene82707.t1;Parent=MS.gene82707 |
| chr3.1 | exon | 38179826 | 38180764 | 38179826 | ID=MS.gene82707.t1.exon1;Parent=MS.gene82707.t1 |
| chr3.1 | CDS | 38179826 | 38180764 | 38179826 | ID=cds.MS.gene82707.t1;Parent=MS.gene82707.t1 |
| Gene Sequence |
| Protein sequence |
