AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44252


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44252.t1 MTR_8g075960 97.814 183 4 0 1 183 1 183 2.66e-132 368
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44252.t1 AT2G17972 46.479 142 69 2 40 181 24 158 7.29e-37 125

Find 51 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAGAAAGGTGCTCTCTTT+TGG 0.312526 8.1:-31465778 MS.gene44252:CDS
GCTTCAATCTTCATAATGAT+TGG 0.317068 8.1:-31465737 MS.gene44252:CDS
CGCCGCAGCCTTAGTTCAAC+TGG 0.318125 8.1:+31465938 None:intergenic
CTCATGTCCTCCCATTTCTT+AGG 0.342760 8.1:+31465833 None:intergenic
GGTGCTACTGCTGCTGCTGC+AGG 0.353627 8.1:+31466024 None:intergenic
ATGGATAGCGTTTAGGTTTG+TGG 0.358027 8.1:-31465711 MS.gene44252:CDS
TTGGGCTATGGATAGCGTTT+AGG 0.362432 8.1:-31465718 MS.gene44252:CDS
CAAAGGCTGCTCCTAAGAAA+TGG 0.371169 8.1:-31465844 MS.gene44252:CDS
TTAGGAGCAGCCTTTGCCTT+GGG 0.384532 8.1:+31465851 None:intergenic
TGGATAGCGTTTAGGTTTGT+GGG 0.391731 8.1:-31465710 MS.gene44252:CDS
TGAGAGGAATTGGTTGTGTC+TGG 0.402863 8.1:+31466084 None:intergenic
AATTGGTTGTGTCTGGTTGA+TGG 0.408980 8.1:+31466091 None:intergenic
TAGGTTGAAATGAGAGGAAT+TGG 0.412261 8.1:+31466074 None:intergenic
ACTGCTGCTGCTGCAGGTGA+TGG 0.419484 8.1:+31466030 None:intergenic
CTTCAATCTTCATAATGATT+GGG 0.425261 8.1:-31465736 MS.gene44252:CDS
CTTAGGAGCAGCCTTTGCCT+TGG 0.425440 8.1:+31465850 None:intergenic
TGCACTTAATATTTATCAGT+TGG 0.425443 8.1:-31465684 MS.gene44252:CDS
GCCAGAAAACAGAGAGAGAA+TGG 0.442782 8.1:-31465899 MS.gene44252:CDS
GGGAAGGTGAGTTTGAAGAG+AGG 0.451276 8.1:+31466152 None:intergenic
GAGCTTTATGTAGGAGAGAA+AGG 0.453819 8.1:-31465791 MS.gene44252:CDS
CTTAGTTCAACTGGTTCAGC+TGG 0.457887 8.1:+31465947 None:intergenic
GGAAGGTGAGTTTGAAGAGA+GGG 0.458101 8.1:+31466153 None:intergenic
TCAACTGGTTCAGCTGGTGT+TGG 0.466879 8.1:+31465953 None:intergenic
GGTTCAGCTGGTGTTGGAGT+TGG 0.493855 8.1:+31465959 None:intergenic
ATCGTCGTTGGTGTTTGTGT+TGG 0.496861 8.1:+31466003 None:intergenic
AAGGCACTTGAGCTTTATGT+AGG 0.500462 8.1:-31465800 MS.gene44252:CDS
TGAGTTTGAAGAGAGGGAGG+TGG 0.502944 8.1:+31466159 None:intergenic
CAGCTGAACCAGTTGAACTA+AGG 0.513049 8.1:-31465946 MS.gene44252:CDS
AAAGGCTGCTCCTAAGAAAT+GGG 0.520681 8.1:-31465843 MS.gene44252:CDS
AGGTGAGTTTGAAGAGAGGG+AGG 0.535848 8.1:+31466156 None:intergenic
AACTGATAAATATTAAGTGC+AGG 0.538359 8.1:+31465686 None:intergenic
GATCCCTTCAGTACATCCTC+AGG 0.542081 8.1:+31465644 None:intergenic
TCTTCATAATGATTGGGCTA+TGG 0.548140 8.1:-31465730 MS.gene44252:CDS
GTCACCTGAGGATGTACTGA+AGG 0.556978 8.1:-31465648 MS.gene44252:CDS
AGTTAGAATAATATTGATGA+GGG 0.564757 8.1:+31466132 None:intergenic
GGACATGAGTTTCACTGAGA+AGG 0.582048 8.1:-31465819 MS.gene44252:CDS
AAGTGGTAGGTTGAAATGAG+AGG 0.590851 8.1:+31466068 None:intergenic
TGGAAAGTGAGGGAAGTGGT+AGG 0.590970 8.1:+31466055 None:intergenic
AGAATAATATTGATGAGGGA+AGG 0.600756 8.1:+31466136 None:intergenic
CATACAGCAACCCAAGGCAA+AGG 0.612470 8.1:-31465861 MS.gene44252:CDS
TAGTTAGAATAATATTGATG+AGG 0.619832 8.1:+31466131 None:intergenic
TGCTGCTGCAGGTGATGGAG+TGG 0.630360 8.1:+31466035 None:intergenic
GGTGATGGAGTGGAAAGTGA+GGG 0.636796 8.1:+31466045 None:intergenic
AACCAGTTGAACTAAGGCTG+CGG 0.639608 8.1:-31465940 MS.gene44252:CDS
ACATCCTCAGGTGACAGTAG+AGG 0.640948 8.1:+31465656 None:intergenic
TCACCTGAGGATGTACTGAA+GGG 0.654909 8.1:-31465647 MS.gene44252:CDS
GGCTGCTCCTAAGAAATGGG+AGG 0.656833 8.1:-31465840 MS.gene44252:CDS
GGAGTGGAAAGTGAGGGAAG+TGG 0.658333 8.1:+31466051 None:intergenic
AAATAGCATACAGCAACCCA+AGG 0.666926 8.1:-31465867 MS.gene44252:CDS
AGGTGATGGAGTGGAAAGTG+AGG 0.701495 8.1:+31466044 None:intergenic
TCAACCTCTACTGTCACCTG+AGG 0.718719 8.1:-31465660 MS.gene44252:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTAGAATAATATTGATGA+GGG + chr8.1:31465674-31465693 None:intergenic 20.0%
!! TAGTTAGAATAATATTGATG+AGG + chr8.1:31465675-31465694 None:intergenic 20.0%
! AACTGATAAATATTAAGTGC+AGG + chr8.1:31466120-31466139 None:intergenic 25.0%
! CTTCAATCTTCATAATGATT+GGG - chr8.1:31466067-31466086 MS.gene44252:CDS 25.0%
! TGCACTTAATATTTATCAGT+TGG - chr8.1:31466119-31466138 MS.gene44252:CDS 25.0%
AGAATAATATTGATGAGGGA+AGG + chr8.1:31465670-31465689 None:intergenic 30.0%
GCTTCAATCTTCATAATGAT+TGG - chr8.1:31466066-31466085 MS.gene44252:CDS 30.0%
TCTTCATAATGATTGGGCTA+TGG - chr8.1:31466073-31466092 MS.gene44252:CDS 35.0%
! TAGGTTGAAATGAGAGGAAT+TGG + chr8.1:31465732-31465751 None:intergenic 35.0%
!! TGAATCTTTTTCATCGTCGT+TGG + chr8.1:31465815-31465834 None:intergenic 35.0%
AAAGGCTGCTCCTAAGAAAT+GGG - chr8.1:31465960-31465979 MS.gene44252:CDS 40.0%
AAATAGCATACAGCAACCCA+AGG - chr8.1:31465936-31465955 MS.gene44252:CDS 40.0%
AATTGGTTGTGTCTGGTTGA+TGG + chr8.1:31465715-31465734 None:intergenic 40.0%
! AAGGCACTTGAGCTTTATGT+AGG - chr8.1:31466003-31466022 MS.gene44252:CDS 40.0%
! AAGTGGTAGGTTGAAATGAG+AGG + chr8.1:31465738-31465757 None:intergenic 40.0%
! ATGGATAGCGTTTAGGTTTG+TGG - chr8.1:31466092-31466111 MS.gene44252:CDS 40.0%
! GAGCTTTATGTAGGAGAGAA+AGG - chr8.1:31466012-31466031 MS.gene44252:CDS 40.0%
! TCCATTCTCTCTCTGTTTTC+TGG + chr8.1:31465908-31465927 None:intergenic 40.0%
! TGGATAGCGTTTAGGTTTGT+GGG - chr8.1:31466093-31466112 MS.gene44252:CDS 40.0%
AACCAGTTGAACTAAGGCTG+CGG - chr8.1:31465863-31465882 MS.gene44252:CDS 45.0%
CAAAGGCTGCTCCTAAGAAA+TGG - chr8.1:31465959-31465978 MS.gene44252:CDS 45.0%
CAGCTGAACCAGTTGAACTA+AGG - chr8.1:31465857-31465876 MS.gene44252:CDS 45.0%
CTCATGTCCTCCCATTTCTT+AGG + chr8.1:31465973-31465992 None:intergenic 45.0%
GCCAGAAAACAGAGAGAGAA+TGG - chr8.1:31465904-31465923 MS.gene44252:CDS 45.0%
GGAAGGTGAGTTTGAAGAGA+GGG + chr8.1:31465653-31465672 None:intergenic 45.0%
GGACATGAGTTTCACTGAGA+AGG - chr8.1:31465984-31466003 MS.gene44252:CDS 45.0%
TCACCTGAGGATGTACTGAA+GGG - chr8.1:31466156-31466175 MS.gene44252:CDS 45.0%
TGAGAGGAATTGGTTGTGTC+TGG + chr8.1:31465722-31465741 None:intergenic 45.0%
TTGGGCTATGGATAGCGTTT+AGG - chr8.1:31466085-31466104 MS.gene44252:CDS 45.0%
! ATCGTCGTTGGTGTTTGTGT+TGG + chr8.1:31465803-31465822 None:intergenic 45.0%
! CTTAGTTCAACTGGTTCAGC+TGG + chr8.1:31465859-31465878 None:intergenic 45.0%
!!! GAGAGAAAGGTGCTCTCTTT+TGG - chr8.1:31466025-31466044 MS.gene44252:CDS 45.0%
ACATCCTCAGGTGACAGTAG+AGG + chr8.1:31466150-31466169 None:intergenic 50.0%
AGGTGAGTTTGAAGAGAGGG+AGG + chr8.1:31465650-31465669 None:intergenic 50.0%
CATACAGCAACCCAAGGCAA+AGG - chr8.1:31465942-31465961 MS.gene44252:CDS 50.0%
GATCCCTTCAGTACATCCTC+AGG + chr8.1:31466162-31466181 None:intergenic 50.0%
GGGAAGGTGAGTTTGAAGAG+AGG + chr8.1:31465654-31465673 None:intergenic 50.0%
GTCACCTGAGGATGTACTGA+AGG - chr8.1:31466155-31466174 MS.gene44252:CDS 50.0%
TCAACCTCTACTGTCACCTG+AGG - chr8.1:31466143-31466162 MS.gene44252:CDS 50.0%
TGAGTTTGAAGAGAGGGAGG+TGG + chr8.1:31465647-31465666 None:intergenic 50.0%
TGGAAAGTGAGGGAAGTGGT+AGG + chr8.1:31465751-31465770 None:intergenic 50.0%
TTAGGAGCAGCCTTTGCCTT+GGG + chr8.1:31465955-31465974 None:intergenic 50.0%
! TCAACTGGTTCAGCTGGTGT+TGG + chr8.1:31465853-31465872 None:intergenic 50.0%
!! AGGTGATGGAGTGGAAAGTG+AGG + chr8.1:31465762-31465781 None:intergenic 50.0%
!! GGTGATGGAGTGGAAAGTGA+GGG + chr8.1:31465761-31465780 None:intergenic 50.0%
CTTAGGAGCAGCCTTTGCCT+TGG + chr8.1:31465956-31465975 None:intergenic 55.0%
GGAGTGGAAAGTGAGGGAAG+TGG + chr8.1:31465755-31465774 None:intergenic 55.0%
GGCTGCTCCTAAGAAATGGG+AGG - chr8.1:31465963-31465982 MS.gene44252:CDS 55.0%
! GGTTCAGCTGGTGTTGGAGT+TGG + chr8.1:31465847-31465866 None:intergenic 55.0%
ACTGCTGCTGCTGCAGGTGA+TGG + chr8.1:31465776-31465795 None:intergenic 60.0%
CGCCGCAGCCTTAGTTCAAC+TGG + chr8.1:31465868-31465887 None:intergenic 60.0%
!! TGCTGCTGCAGGTGATGGAG+TGG + chr8.1:31465771-31465790 None:intergenic 60.0%
!! GGTGCTACTGCTGCTGCTGC+AGG + chr8.1:31465782-31465801 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 31465637 31466188 31465637 ID=MS.gene44252
chr8.1 mRNA 31465637 31466188 31465637 ID=MS.gene44252.t1;Parent=MS.gene44252
chr8.1 exon 31465637 31466188 31465637 ID=MS.gene44252.t1.exon1;Parent=MS.gene44252.t1
chr8.1 CDS 31465637 31466188 31465637 ID=cds.MS.gene44252.t1;Parent=MS.gene44252.t1
Gene Sequence

>MS.gene44252

ATGGGAACCACCTCCCTCTCTTCAAACTCACCTTCCCTCATCAATATTATTCTAACTAGACGTTCAAGTTCACTACCATCAACCAGACACAACCAATTCCTCTCATTTCAACCTACCACTTCCCTCACTTTCCACTCCATCACCTGCAGCAGCAGCAGTAGCACCAACACAAACACCAACGACGATGAAAAAGATTCAGTTCAAGCACCAACTCCAACACCAGCTGAACCAGTTGAACTAAGGCTGCGGCGAAGATCAAAAAGACAAGCCAGAAAACAGAGAGAGAATGGAACTCTCACAAATAGCATACAGCAACCCAAGGCAAAGGCTGCTCCTAAGAAATGGGAGGACATGAGTTTCACTGAGAAGGCACTTGAGCTTTATGTAGGAGAGAAAGGTGCTCTCTTTTGGCTTAACAAATTTGCATATGCTTCAATCTTCATAATGATTGGGCTATGGATAGCGTTTAGGTTTGTGGGTCCTGCACTTAATATTTATCAGTTGGATCAACCTCTACTGTCACCTGAGGATGTACTGAAGGGATCTTCCTGA

Protein sequence

>MS.gene44252.t1

MGTTSLSSNSPSLINIILTRRSSSLPSTRHNQFLSFQPTTSLTFHSITCSSSSSTNTNTNDDEKDSVQAPTPTPAEPVELRLRRRSKRQARKQRENGTLTNSIQQPKAKAAPKKWEDMSFTEKALELYVGEKGALFWLNKFAYASIFIMIGLWIAFRFVGPALNIYQLDQPLLSPEDVLKGSS