Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56084.t1 | PNY02770.1 | 92.2 | 335 | 26 | 0 | 1 | 335 | 1 | 335 | 6.00E-62 | 248.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56084.t1 | Q9SZL6 | 77.5 | 324 | 71 | 2 | 8 | 329 | 5 | 328 | 1.1e-24 | 115.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56084.t1 | A0A2K3NIC0 | 92.2 | 335 | 26 | 0 | 1 | 335 | 1 | 335 | 4.3e-62 | 248.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56084.t1 | TR | mTERF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene56084 | 0.833296 | 5.79E-56 | -1.69E-46 |
| MS.gene049351 | MS.gene56084 | 0.815957 | 7.08E-52 | -1.69E-46 |
| MS.gene049389 | MS.gene56084 | 0.80353 | 3.34E-49 | -1.69E-46 |
| MS.gene049491 | MS.gene56084 | 0.850496 | 1.64E-60 | -1.69E-46 |
| MS.gene049758 | MS.gene56084 | 0.867377 | 1.43E-65 | -1.69E-46 |
| MS.gene050076 | MS.gene56084 | 0.810971 | 8.84E-51 | -1.69E-46 |
| MS.gene050099 | MS.gene56084 | 0.850619 | 1.51E-60 | -1.69E-46 |
| MS.gene050301 | MS.gene56084 | 0.891269 | 4.63E-74 | -1.69E-46 |
| MS.gene050558 | MS.gene56084 | 0.821116 | 4.79E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene56084 | 0.858555 | 7.60E-63 | -1.69E-46 |
| MS.gene051471 | MS.gene56084 | 0.833446 | 5.31E-56 | -1.69E-46 |
| MS.gene051582 | MS.gene56084 | 0.804481 | 2.12E-49 | -1.69E-46 |
| MS.gene051662 | MS.gene56084 | 0.823697 | 1.20E-53 | -1.69E-46 |
| MS.gene051663 | MS.gene56084 | 0.848594 | 5.55E-60 | -1.69E-46 |
| MS.gene051860 | MS.gene56084 | 0.81877 | 1.65E-52 | -1.69E-46 |
| MS.gene052078 | MS.gene56084 | 0.84743 | 1.16E-59 | -1.69E-46 |
| MS.gene052178 | MS.gene56084 | 0.845651 | 3.56E-59 | -1.69E-46 |
| MS.gene052188 | MS.gene56084 | 0.832991 | 6.89E-56 | -1.69E-46 |
| MS.gene052207 | MS.gene56084 | 0.820024 | 8.53E-53 | -1.69E-46 |
| MS.gene052253 | MS.gene56084 | 0.820908 | 5.35E-53 | -1.69E-46 |
| MS.gene052287 | MS.gene56084 | 0.807465 | 4.99E-50 | -1.69E-46 |
| MS.gene052310 | MS.gene56084 | 0.847649 | 1.01E-59 | -1.69E-46 |
| MS.gene052326 | MS.gene56084 | 0.848655 | 5.34E-60 | -1.69E-46 |
| MS.gene052340 | MS.gene56084 | 0.833742 | 4.48E-56 | -1.69E-46 |
| MS.gene052600 | MS.gene56084 | 0.811839 | 5.73E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene56084 | 0.806379 | 8.46E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene56084 | 0.826143 | 3.19E-54 | -1.69E-46 |
| MS.gene053836 | MS.gene56084 | 0.872305 | 3.52E-67 | -1.69E-46 |
| MS.gene054123 | MS.gene56084 | 0.81356 | 2.41E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene56084 | 0.833446 | 5.31E-56 | -1.69E-46 |
| MS.gene05455 | MS.gene56084 | 0.837406 | 5.30E-57 | -1.69E-46 |
| MS.gene054942 | MS.gene56084 | 0.822889 | 1.86E-53 | -1.69E-46 |
| MS.gene054983 | MS.gene56084 | 0.801585 | 8.40E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene56084 | 0.810015 | 1.42E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene56084 | 0.848579 | 5.60E-60 | -1.69E-46 |
| MS.gene055390 | MS.gene56084 | 0.848455 | 6.06E-60 | -1.69E-46 |
| MS.gene055422 | MS.gene56084 | 0.871768 | 5.31E-67 | -1.69E-46 |
| MS.gene055428 | MS.gene56084 | 0.87891 | 1.92E-69 | -1.69E-46 |
| MS.gene055604 | MS.gene56084 | 0.814638 | 1.39E-51 | -1.69E-46 |
| MS.gene055712 | MS.gene56084 | 0.80841 | 3.14E-50 | -1.69E-46 |
| MS.gene056314 | MS.gene56084 | 0.810086 | 1.37E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene56084 | 0.83105 | 2.08E-55 | -1.69E-46 |
| MS.gene056875 | MS.gene56084 | 0.80639 | 8.42E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene56084 | 0.830505 | 2.83E-55 | -1.69E-46 |
| MS.gene057121 | MS.gene56084 | 0.852504 | 4.42E-61 | -1.69E-46 |
| MS.gene057245 | MS.gene56084 | 0.822503 | 2.29E-53 | -1.69E-46 |
| MS.gene05733 | MS.gene56084 | 0.840575 | 8.00E-58 | -1.69E-46 |
| MS.gene057439 | MS.gene56084 | 0.817398 | 3.37E-52 | -1.69E-46 |
| MS.gene057450 | MS.gene56084 | 0.8014 | 9.17E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene56084 | 0.886376 | 3.61E-72 | -1.69E-46 |
| MS.gene05811 | MS.gene56084 | 0.884681 | 1.56E-71 | -1.69E-46 |
| MS.gene058142 | MS.gene56084 | 0.846227 | 2.48E-59 | -1.69E-46 |
| MS.gene058233 | MS.gene56084 | 0.806344 | 8.61E-50 | -1.69E-46 |
| MS.gene058276 | MS.gene56084 | 0.813901 | 2.02E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene56084 | 0.839645 | 1.40E-57 | -1.69E-46 |
| MS.gene058749 | MS.gene56084 | 0.815322 | 9.81E-52 | -1.69E-46 |
| MS.gene058833 | MS.gene56084 | 0.846953 | 1.57E-59 | -1.69E-46 |
| MS.gene058845 | MS.gene56084 | 0.8401 | 1.07E-57 | -1.69E-46 |
| MS.gene05907 | MS.gene56084 | 0.853178 | 2.84E-61 | -1.69E-46 |
| MS.gene059518 | MS.gene56084 | 0.881158 | 3.03E-70 | -1.69E-46 |
| MS.gene059597 | MS.gene56084 | 0.856342 | 3.43E-62 | -1.69E-46 |
| MS.gene059676 | MS.gene56084 | 0.80919 | 2.14E-50 | -1.69E-46 |
| MS.gene059688 | MS.gene56084 | 0.818947 | 1.50E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene56084 | 0.838285 | 3.15E-57 | -1.69E-46 |
| MS.gene059695 | MS.gene56084 | 0.834968 | 2.21E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene56084 | 0.860841 | 1.56E-63 | -1.69E-46 |
| MS.gene059894 | MS.gene56084 | 0.857001 | 2.20E-62 | -1.69E-46 |
| MS.gene059899 | MS.gene56084 | 0.811658 | 6.27E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene56084 | 0.849502 | 3.10E-60 | -1.69E-46 |
| MS.gene060407 | MS.gene56084 | 0.83307 | 6.59E-56 | -1.69E-46 |
| MS.gene060476 | MS.gene56084 | 0.835292 | 1.83E-56 | -1.69E-46 |
| MS.gene061049 | MS.gene56084 | 0.854114 | 1.53E-61 | -1.69E-46 |
| MS.gene061112 | MS.gene56084 | 0.806683 | 7.30E-50 | -1.69E-46 |
| MS.gene061265 | MS.gene56084 | 0.803057 | 4.18E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene56084 | MS.gene77650 | PPI |
| MS.gene56084 | MS.gene039043 | PPI |
| MS.gene92189 | MS.gene56084 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56084.t1 | MTR_4g084630 | 96.657 | 329 | 11 | 0 | 7 | 335 | 4 | 332 | 0.0 | 654 |
| MS.gene56084.t1 | MTR_6g003960 | 27.010 | 311 | 191 | 4 | 49 | 325 | 283 | 591 | 2.24e-34 | 133 |
| MS.gene56084.t1 | MTR_5g068860 | 24.333 | 300 | 223 | 4 | 24 | 322 | 187 | 483 | 1.11e-31 | 124 |
| MS.gene56084.t1 | MTR_5g068860 | 22.727 | 220 | 161 | 3 | 46 | 265 | 141 | 351 | 1.38e-13 | 72.0 |
| MS.gene56084.t1 | MTR_4g070060 | 25.850 | 294 | 213 | 5 | 31 | 322 | 296 | 586 | 4.99e-31 | 123 |
| MS.gene56084.t1 | MTR_2g034600 | 26.374 | 273 | 195 | 4 | 50 | 322 | 217 | 483 | 9.04e-27 | 110 |
| MS.gene56084.t1 | MTR_2g034600 | 29.605 | 152 | 105 | 2 | 112 | 263 | 207 | 356 | 3.31e-14 | 73.6 |
| MS.gene56084.t1 | MTR_2g034600 | 23.451 | 226 | 162 | 3 | 4 | 226 | 278 | 495 | 1.08e-12 | 68.9 |
| MS.gene56084.t1 | MTR_2g034600 | 26.374 | 273 | 195 | 4 | 50 | 322 | 282 | 548 | 1.23e-26 | 110 |
| MS.gene56084.t1 | MTR_2g034600 | 29.605 | 152 | 105 | 2 | 112 | 263 | 272 | 421 | 3.76e-14 | 73.6 |
| MS.gene56084.t1 | MTR_2g034600 | 23.451 | 226 | 162 | 3 | 4 | 226 | 343 | 560 | 1.13e-12 | 68.9 |
| MS.gene56084.t1 | MTR_2g034600 | 26.374 | 273 | 195 | 4 | 50 | 322 | 278 | 544 | 1.24e-26 | 110 |
| MS.gene56084.t1 | MTR_2g034600 | 29.605 | 152 | 105 | 2 | 112 | 263 | 268 | 417 | 3.99e-14 | 73.6 |
| MS.gene56084.t1 | MTR_2g034600 | 23.451 | 226 | 162 | 3 | 4 | 226 | 339 | 556 | 1.28e-12 | 68.9 |
| MS.gene56084.t1 | MTR_1g115415 | 22.115 | 312 | 199 | 5 | 50 | 322 | 145 | 451 | 5.86e-25 | 105 |
| MS.gene56084.t1 | MTR_1g115415 | 24.675 | 231 | 148 | 3 | 48 | 253 | 82 | 311 | 1.54e-16 | 80.9 |
| MS.gene56084.t1 | MTR_5g094610 | 27.820 | 266 | 178 | 6 | 45 | 301 | 188 | 448 | 1.78e-23 | 101 |
| MS.gene56084.t1 | MTR_5g094610 | 27.948 | 229 | 161 | 3 | 44 | 271 | 226 | 451 | 3.77e-19 | 88.6 |
| MS.gene56084.t1 | MTR_2g049780 | 23.600 | 250 | 181 | 4 | 72 | 321 | 65 | 304 | 1.15e-17 | 82.8 |
| MS.gene56084.t1 | MTR_7g081270 | 29.032 | 217 | 134 | 5 | 119 | 325 | 77 | 283 | 1.19e-16 | 79.3 |
| MS.gene56084.t1 | MTR_5g041630 | 26.667 | 225 | 150 | 3 | 100 | 318 | 74 | 289 | 4.34e-16 | 78.2 |
| MS.gene56084.t1 | MTR_2g067230 | 28.758 | 153 | 103 | 1 | 170 | 322 | 154 | 300 | 4.61e-16 | 78.2 |
| MS.gene56084.t1 | MTR_2g437130 | 22.892 | 332 | 235 | 9 | 3 | 327 | 38 | 355 | 3.30e-13 | 70.1 |
| MS.gene56084.t1 | MTR_8g012210 | 21.269 | 268 | 199 | 5 | 20 | 287 | 63 | 318 | 7.57e-12 | 66.2 |
| MS.gene56084.t1 | MTR_8g105560 | 20.307 | 261 | 186 | 6 | 79 | 322 | 121 | 376 | 1.09e-11 | 66.2 |
| MS.gene56084.t1 | MTR_2g438020 | 20.124 | 323 | 241 | 8 | 11 | 325 | 106 | 419 | 4.52e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56084.t1 | AT4G38160 | 66.154 | 325 | 108 | 2 | 7 | 329 | 4 | 328 | 2.63e-161 | 454 |
| MS.gene56084.t1 | AT4G38160 | 66.154 | 325 | 108 | 2 | 7 | 329 | 4 | 328 | 2.63e-161 | 454 |
| MS.gene56084.t1 | AT4G38160 | 66.154 | 325 | 108 | 2 | 7 | 329 | 4 | 328 | 1.07e-160 | 453 |
| MS.gene56084.t1 | AT4G38160 | 66.154 | 325 | 108 | 2 | 7 | 329 | 4 | 328 | 4.73e-160 | 452 |
| MS.gene56084.t1 | AT2G21710 | 25.776 | 322 | 199 | 8 | 40 | 324 | 277 | 595 | 5.80e-33 | 129 |
| MS.gene56084.t1 | AT1G78930 | 29.368 | 269 | 184 | 4 | 67 | 332 | 323 | 588 | 2.07e-31 | 124 |
| MS.gene56084.t1 | AT4G02990 | 21.935 | 310 | 204 | 3 | 49 | 322 | 184 | 491 | 3.38e-28 | 115 |
| MS.gene56084.t1 | AT4G02990 | 21.147 | 279 | 189 | 4 | 17 | 268 | 92 | 366 | 6.40e-16 | 79.0 |
| MS.gene56084.t1 | AT4G02990 | 21.935 | 310 | 204 | 3 | 49 | 322 | 184 | 491 | 3.38e-28 | 115 |
| MS.gene56084.t1 | AT4G02990 | 21.147 | 279 | 189 | 4 | 17 | 268 | 92 | 366 | 6.40e-16 | 79.0 |
| MS.gene56084.t1 | AT4G14605 | 27.757 | 263 | 184 | 4 | 60 | 322 | 212 | 468 | 1.22e-27 | 113 |
| MS.gene56084.t1 | AT4G14605 | 23.786 | 206 | 149 | 2 | 7 | 212 | 266 | 463 | 8.00e-14 | 72.4 |
| MS.gene56084.t1 | AT4G14605 | 27.757 | 263 | 184 | 4 | 60 | 322 | 212 | 468 | 1.22e-27 | 113 |
| MS.gene56084.t1 | AT4G14605 | 23.786 | 206 | 149 | 2 | 7 | 212 | 266 | 463 | 8.00e-14 | 72.4 |
| MS.gene56084.t1 | AT2G44020 | 24.679 | 312 | 191 | 5 | 50 | 322 | 150 | 456 | 4.68e-27 | 111 |
| MS.gene56084.t1 | AT2G44020 | 23.318 | 223 | 166 | 2 | 46 | 268 | 114 | 331 | 4.35e-16 | 79.3 |
| MS.gene56084.t1 | AT2G21710 | 25.442 | 283 | 171 | 8 | 40 | 285 | 277 | 556 | 4.66e-24 | 103 |
| MS.gene56084.t1 | AT2G21710 | 25.623 | 281 | 169 | 8 | 40 | 283 | 277 | 554 | 8.60e-24 | 102 |
| MS.gene56084.t1 | AT2G03050 | 27.004 | 237 | 166 | 4 | 87 | 322 | 39 | 269 | 2.77e-23 | 97.8 |
| MS.gene56084.t1 | AT2G03050 | 27.004 | 237 | 166 | 4 | 87 | 322 | 40 | 270 | 2.79e-23 | 97.8 |
| MS.gene56084.t1 | AT5G55580 | 25.475 | 263 | 176 | 6 | 49 | 301 | 83 | 335 | 9.77e-22 | 95.1 |
| MS.gene56084.t1 | AT5G55580 | 25.217 | 230 | 166 | 4 | 44 | 271 | 113 | 338 | 1.02e-18 | 86.7 |
| MS.gene56084.t1 | AT5G55580 | 25.475 | 263 | 176 | 6 | 49 | 301 | 189 | 441 | 2.49e-21 | 95.1 |
| MS.gene56084.t1 | AT5G55580 | 25.217 | 230 | 166 | 4 | 44 | 271 | 219 | 444 | 2.91e-18 | 85.9 |
| MS.gene56084.t1 | AT2G36000 | 26.562 | 192 | 134 | 2 | 131 | 321 | 122 | 307 | 1.98e-19 | 87.8 |
| MS.gene56084.t1 | AT2G36000 | 26.562 | 192 | 134 | 2 | 131 | 321 | 122 | 307 | 2.90e-19 | 87.4 |
| MS.gene56084.t1 | AT2G34620 | 23.529 | 204 | 154 | 1 | 119 | 322 | 90 | 291 | 6.78e-19 | 85.9 |
| MS.gene56084.t1 | AT2G34620 | 29.333 | 150 | 102 | 3 | 50 | 198 | 93 | 239 | 3.82e-12 | 66.6 |
| MS.gene56084.t1 | AT3G18870 | 29.851 | 201 | 135 | 5 | 127 | 325 | 76 | 272 | 4.18e-18 | 83.2 |
| MS.gene56084.t1 | AT5G54180 | 27.481 | 262 | 179 | 9 | 64 | 321 | 234 | 488 | 7.05e-18 | 84.7 |
| MS.gene56084.t1 | AT5G55580 | 24.859 | 177 | 130 | 3 | 44 | 218 | 219 | 394 | 2.96e-14 | 73.6 |
| MS.gene56084.t1 | AT5G55580 | 25.116 | 215 | 141 | 6 | 49 | 253 | 189 | 393 | 6.50e-13 | 69.3 |
| MS.gene56084.t1 | AT5G64950 | 29.448 | 163 | 108 | 5 | 93 | 253 | 52 | 209 | 4.79e-11 | 63.9 |
| MS.gene56084.t1 | AT1G21150 | 19.392 | 263 | 199 | 5 | 5 | 264 | 148 | 400 | 5.03e-11 | 63.9 |
| MS.gene56084.t1 | AT1G21150 | 19.392 | 263 | 199 | 5 | 5 | 264 | 84 | 336 | 6.06e-11 | 63.5 |
| MS.gene56084.t1 | AT1G21150 | 19.392 | 263 | 199 | 5 | 5 | 264 | 48 | 300 | 7.14e-11 | 63.2 |
Find 50 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGACCAAGTACAAGAATTT+TGG | 0.151290 | 4.4:-28077297 | None:intergenic |
| GATTAAGGACCCGTATATTA+TGG | 0.175262 | 4.4:+28077613 | MS.gene56084:CDS |
| GCGAACGAGCCAATGAAAAT+TGG | 0.202887 | 4.4:+28077213 | MS.gene56084:CDS |
| AGAGAAGCTTTGTCCTCTTT+TGG | 0.259572 | 4.4:+28077430 | MS.gene56084:CDS |
| CAAGAGTGTTCGGAATTCTT+TGG | 0.260167 | 4.4:+28077853 | MS.gene56084:CDS |
| ACACTATAACCCATAATATA+CGG | 0.287612 | 4.4:-28077623 | None:intergenic |
| CGAACACTCTTGATCAAAAT+AGG | 0.322211 | 4.4:-28077842 | None:intergenic |
| TTTGACTGAGCAGGATCTTC+AGG | 0.331599 | 4.4:+28077691 | MS.gene56084:CDS |
| TTAAAGAACTGTGGATTTCA+TGG | 0.334993 | 4.4:+28077785 | MS.gene56084:CDS |
| AAGAAGTTCCACTTGAAGTT+TGG | 0.341154 | 4.4:+28078046 | MS.gene56084:CDS |
| TTGAAGTTTGGCTTGTTAGA+AGG | 0.350213 | 4.4:+28078058 | MS.gene56084:CDS |
| TGGGAGTATTTGAAAACAAT+TGG | 0.351216 | 4.4:+28077233 | MS.gene56084:CDS |
| TAGAGATGTGGATAAGGTTT+TGG | 0.354515 | 4.4:+28077745 | MS.gene56084:CDS |
| TGGGTTATAGTGTTGATAAA+AGG | 0.361012 | 4.4:+28077633 | MS.gene56084:CDS |
| TGGCCGGCAGATAGTTGATT+TGG | 0.380260 | 4.4:+28077805 | MS.gene56084:CDS |
| TGCCCCAAAATTCTTGTACT+TGG | 0.381501 | 4.4:+28077293 | MS.gene56084:CDS |
| ATTAAGGACCCGTATATTAT+GGG | 0.382034 | 4.4:+28077614 | MS.gene56084:CDS |
| CTTGATAAAGACGGAATGAT+TGG | 0.389340 | 4.4:+28077581 | MS.gene56084:CDS |
| GCTGCTGTTATTGCCGCTAC+TGG | 0.445431 | 4.4:-28077099 | None:intergenic |
| CGAACGAGCCAATGAAAATT+GGG | 0.448262 | 4.4:+28077214 | MS.gene56084:CDS |
| TTTGAGTAGAGATGTGGATA+AGG | 0.454342 | 4.4:+28077739 | MS.gene56084:CDS |
| CAGATAGTTGATTTGGTGGT+TGG | 0.459004 | 4.4:+28077812 | MS.gene56084:CDS |
| GTGAAATTTCTTGCTAGTGT+TGG | 0.461499 | 4.4:+28077557 | MS.gene56084:CDS |
| ATGCTATAACTAATAAGCCT+AGG | 0.489054 | 4.4:-28077512 | None:intergenic |
| CAATGAAAAGATTGTTCCAA+TGG | 0.489343 | 4.4:+28077319 | MS.gene56084:CDS |
| TTGCAGCTTGAGTGAAATGC+TGG | 0.494640 | 4.4:+28078012 | MS.gene56084:CDS |
| TATCCATGCTTCTTTCGTCA+TGG | 0.501505 | 4.4:+28077935 | MS.gene56084:CDS |
| GTCGATAGGTTTGACTGAGC+AGG | 0.509092 | 4.4:+28077682 | MS.gene56084:CDS |
| CCGGCAGATAGTTGATTTGG+TGG | 0.512541 | 4.4:+28077808 | MS.gene56084:CDS |
| ACACTACAAGAGAGTATTTG+AGG | 0.513102 | 4.4:-28077404 | None:intergenic |
| AGAACTGTGGATTTCATGGC+CGG | 0.517285 | 4.4:+28077789 | MS.gene56084:CDS |
| CACTATAACCCATAATATAC+GGG | 0.530664 | 4.4:-28077622 | None:intergenic |
| CACTACAAGAGAGTATTTGA+GGG | 0.546905 | 4.4:-28077403 | None:intergenic |
| TGCTATAACTAATAAGCCTA+GGG | 0.547400 | 4.4:-28077511 | None:intergenic |
| TAAAAGGCTTCGTCCTACTT+CGG | 0.555828 | 4.4:+28077649 | MS.gene56084:CDS |
| CTAACAAGCCAAACTTCAAG+TGG | 0.558319 | 4.4:-28078054 | None:intergenic |
| GTGTTGAGACATTCAACCAT+TGG | 0.559854 | 4.4:-28077335 | None:intergenic |
| TTCGGAATTCTTTGGAACCG+AGG | 0.584467 | 4.4:+28077861 | MS.gene56084:CDS |
| TCAAATACTCTCTTGTAGTG+TGG | 0.603146 | 4.4:+28077406 | MS.gene56084:CDS |
| ATAGCAGATGCAACTTCATG+AGG | 0.606629 | 4.4:-28077371 | None:intergenic |
| AGGACATACTTTGAGTAACT+AGG | 0.611172 | 4.4:+28078078 | MS.gene56084:CDS |
| AGGCCATGACGAAAGAAGCA+TGG | 0.620961 | 4.4:-28077938 | None:intergenic |
| ACACTCTTGATCAAAATAGG+AGG | 0.622517 | 4.4:-28077839 | None:intergenic |
| CCACCAAATCAACTATCTGC+CGG | 0.624823 | 4.4:-28077808 | None:intergenic |
| TAGCATTGAGACGAAGATGA+CGG | 0.632455 | 4.4:+28077529 | MS.gene56084:CDS |
| GGCACAAGCTCTTAAAGCAG+AGG | 0.635201 | 4.4:+28077981 | MS.gene56084:CDS |
| AGTGTTGGTCTTGATAAAGA+CGG | 0.642183 | 4.4:+28077572 | MS.gene56084:CDS |
| AGTAAAATGGAAACCAGTAG+CGG | 0.644038 | 4.4:+28077086 | None:intergenic |
| ACATCTCTACTCAAAATCGA+CGG | 0.644998 | 4.4:-28077731 | None:intergenic |
| ATAACAGCAGCAACATGATG+TGG | 0.646285 | 4.4:+28077111 | MS.gene56084:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTGGAAAAATTATGATTA+AGG | + | chr4.4:28077598-28077617 | MS.gene56084:CDS | 20.0% |
| !!! | AAATTTTATTGTTAAACCCT+AGG | + | chr4.4:28077495-28077514 | MS.gene56084:CDS | 20.0% |
| !!! | TATGATTTTTTAAAGAACTG+TGG | + | chr4.4:28077776-28077795 | MS.gene56084:CDS | 20.0% |
| ! | ACACTATAACCCATAATATA+CGG | - | chr4.4:28077626-28077645 | None:intergenic | 25.0% |
| !!! | AAGTTTTTGGTAGATGTAAT+GGG | + | chr4.4:28077887-28077906 | MS.gene56084:CDS | 25.0% |
| !!! | AATTTTCCCGATTTGTTTTT+CGG | - | chr4.4:28077480-28077499 | None:intergenic | 25.0% |
| !!! | TGTGAACATTTTTTGTATGA+TGG | - | chr4.4:28077165-28077184 | None:intergenic | 25.0% |
| !!! | TTGGCATTTTTTCAAACATT+AGG | + | chr4.4:28077449-28077468 | MS.gene56084:CDS | 25.0% |
| ATGCTATAACTAATAAGCCT+AGG | - | chr4.4:28077515-28077534 | None:intergenic | 30.0% | |
| ATTAAGGACCCGTATATTAT+GGG | + | chr4.4:28077614-28077633 | MS.gene56084:CDS | 30.0% | |
| CAATGAAAAGATTGTTCCAA+TGG | + | chr4.4:28077319-28077338 | MS.gene56084:CDS | 30.0% | |
| CACTATAACCCATAATATAC+GGG | - | chr4.4:28077625-28077644 | None:intergenic | 30.0% | |
| GTTTGAAAAAATGCCAAAAG+AGG | - | chr4.4:28077446-28077465 | None:intergenic | 30.0% | |
| TGCTATAACTAATAAGCCTA+GGG | - | chr4.4:28077514-28077533 | None:intergenic | 30.0% | |
| TTAAAGAACTGTGGATTTCA+TGG | + | chr4.4:28077785-28077804 | MS.gene56084:CDS | 30.0% | |
| ! | AGACCAAGTACAAGAATTTT+GGG | - | chr4.4:28077299-28077318 | None:intergenic | 30.0% |
| ! | CAAATACTCCCAATTTTCAT+TGG | - | chr4.4:28077225-28077244 | None:intergenic | 30.0% |
| !! | ATTTTCCCGATTTGTTTTTC+GGG | - | chr4.4:28077479-28077498 | None:intergenic | 30.0% |
| !! | TGGGAGTATTTGAAAACAAT+TGG | + | chr4.4:28077233-28077252 | MS.gene56084:CDS | 30.0% |
| !! | TGGGTTATAGTGTTGATAAA+AGG | + | chr4.4:28077633-28077652 | MS.gene56084:CDS | 30.0% |
| !!! | CAAGTTTTTGGTAGATGTAA+TGG | + | chr4.4:28077886-28077905 | MS.gene56084:CDS | 30.0% |
| !!! | CTATTTTGATCAAGAGTGTT+CGG | + | chr4.4:28077843-28077862 | MS.gene56084:CDS | 30.0% |
| AAGAAGTTCCACTTGAAGTT+TGG | + | chr4.4:28078046-28078065 | MS.gene56084:CDS | 35.0% | |
| ACACTCTTGATCAAAATAGG+AGG | - | chr4.4:28077842-28077861 | None:intergenic | 35.0% | |
| ACATCTCTACTCAAAATCGA+CGG | - | chr4.4:28077734-28077753 | None:intergenic | 35.0% | |
| CGAACACTCTTGATCAAAAT+AGG | - | chr4.4:28077845-28077864 | None:intergenic | 35.0% | |
| CTTGATAAAGACGGAATGAT+TGG | + | chr4.4:28077581-28077600 | MS.gene56084:CDS | 35.0% | |
| GATTAAGGACCCGTATATTA+TGG | + | chr4.4:28077613-28077632 | MS.gene56084:CDS | 35.0% | |
| GTGAAATTTCTTGCTAGTGT+TGG | + | chr4.4:28077557-28077576 | MS.gene56084:CDS | 35.0% | |
| TCAAATACTCTCTTGTAGTG+TGG | + | chr4.4:28077406-28077425 | MS.gene56084:CDS | 35.0% | |
| TCATGGCCTGAAGAAAAAAT+TGG | + | chr4.4:28077952-28077971 | MS.gene56084:CDS | 35.0% | |
| TCTACCAAAAACTTGATCCT+CGG | - | chr4.4:28077881-28077900 | None:intergenic | 35.0% | |
| TTTGAGTAGAGATGTGGATA+AGG | + | chr4.4:28077739-28077758 | MS.gene56084:CDS | 35.0% | |
| ! | CAACATGATGTGGTTTTTCA+AGG | + | chr4.4:28077121-28077140 | MS.gene56084:CDS | 35.0% |
| ! | GACCAAGTACAAGAATTTTG+GGG | - | chr4.4:28077298-28077317 | None:intergenic | 35.0% |
| ! | GAGACCAAGTACAAGAATTT+TGG | - | chr4.4:28077300-28077319 | None:intergenic | 35.0% |
| ! | TAGAGATGTGGATAAGGTTT+TGG | + | chr4.4:28077745-28077764 | MS.gene56084:CDS | 35.0% |
| !! | ACACTACAAGAGAGTATTTG+AGG | - | chr4.4:28077407-28077426 | None:intergenic | 35.0% |
| !! | AGTGTTGGTCTTGATAAAGA+CGG | + | chr4.4:28077572-28077591 | MS.gene56084:CDS | 35.0% |
| !! | CACTACAAGAGAGTATTTGA+GGG | - | chr4.4:28077406-28077425 | None:intergenic | 35.0% |
| !! | TGAAGAAAAAATTGGTGTCG+CGG | + | chr4.4:28077960-28077979 | MS.gene56084:CDS | 35.0% |
| !! | TTGAAGTTTGGCTTGTTAGA+AGG | + | chr4.4:28078058-28078077 | MS.gene56084:CDS | 35.0% |
| !!! | GGGCATTTTGAAACAATTGA+AGG | - | chr4.4:28077278-28077297 | None:intergenic | 35.0% |
| ATAACAGCAGCAACATGATG+TGG | + | chr4.4:28077111-28077130 | MS.gene56084:CDS | 40.0% | |
| ATAGCAGATGCAACTTCATG+AGG | - | chr4.4:28077374-28077393 | None:intergenic | 40.0% | |
| CGAACGAGCCAATGAAAATT+GGG | + | chr4.4:28077214-28077233 | MS.gene56084:CDS | 40.0% | |
| CTAACAAGCCAAACTTCAAG+TGG | - | chr4.4:28078057-28078076 | None:intergenic | 40.0% | |
| GACTTCAAAAACTCCGAAGT+AGG | - | chr4.4:28077665-28077684 | None:intergenic | 40.0% | |
| GTGTTGAGACATTCAACCAT+TGG | - | chr4.4:28077338-28077357 | None:intergenic | 40.0% | |
| TAAAAGGCTTCGTCCTACTT+CGG | + | chr4.4:28077649-28077668 | MS.gene56084:CDS | 40.0% | |
| TATCCATGCTTCTTTCGTCA+TGG | + | chr4.4:28077935-28077954 | MS.gene56084:CDS | 40.0% | |
| TGCCCCAAAATTCTTGTACT+TGG | + | chr4.4:28077293-28077312 | MS.gene56084:CDS | 40.0% | |
| ! | TAGCATTGAGACGAAGATGA+CGG | + | chr4.4:28077529-28077548 | MS.gene56084:CDS | 40.0% |
| !! | AGAGAAGCTTTGTCCTCTTT+TGG | + | chr4.4:28077430-28077449 | MS.gene56084:CDS | 40.0% |
| !! | CAAGAGTGTTCGGAATTCTT+TGG | + | chr4.4:28077853-28077872 | MS.gene56084:CDS | 40.0% |
| !! | CAGATAGTTGATTTGGTGGT+TGG | + | chr4.4:28077812-28077831 | MS.gene56084:CDS | 40.0% |
| !! | GTCGATTTTGAGTAGAGATG+TGG | + | chr4.4:28077733-28077752 | MS.gene56084:CDS | 40.0% |
| !!! | ATGTGGTTTTTCAAGGACAG+AGG | + | chr4.4:28077128-28077147 | MS.gene56084:CDS | 40.0% |
| !!! | GCGACACCAATTTTTTCTTC+AGG | - | chr4.4:28077961-28077980 | None:intergenic | 40.0% |
| !!! | TCGGAGTTTTTGAAGTCGAT+AGG | + | chr4.4:28077668-28077687 | MS.gene56084:CDS | 40.0% |
| AGAACTGTGGATTTCATGGC+CGG | + | chr4.4:28077789-28077808 | MS.gene56084:CDS | 45.0% | |
| CCACCAAATCAACTATCTGC+CGG | - | chr4.4:28077811-28077830 | None:intergenic | 45.0% | |
| GCGAACGAGCCAATGAAAAT+TGG | + | chr4.4:28077213-28077232 | MS.gene56084:CDS | 45.0% | |
| GGTGTGCCCGAAAAACAAAT+CGG | + | chr4.4:28077470-28077489 | MS.gene56084:CDS | 45.0% | |
| GTGTGCCCGAAAAACAAATC+GGG | + | chr4.4:28077471-28077490 | MS.gene56084:CDS | 45.0% | |
| TTGCAGCTTGAGTGAAATGC+TGG | + | chr4.4:28078012-28078031 | MS.gene56084:CDS | 45.0% | |
| TTTGACTGAGCAGGATCTTC+AGG | + | chr4.4:28077691-28077710 | MS.gene56084:CDS | 45.0% | |
| ! | GGAACCGAGGATCAAGTTTT+TGG | + | chr4.4:28077874-28077893 | MS.gene56084:CDS | 45.0% |
| !! | TTCGGAATTCTTTGGAACCG+AGG | + | chr4.4:28077861-28077880 | MS.gene56084:CDS | 45.0% |
| AGGCCATGACGAAAGAAGCA+TGG | - | chr4.4:28077941-28077960 | None:intergenic | 50.0% | |
| GGCACAAGCTCTTAAAGCAG+AGG | + | chr4.4:28077981-28078000 | MS.gene56084:CDS | 50.0% | |
| GTCGATAGGTTTGACTGAGC+AGG | + | chr4.4:28077682-28077701 | MS.gene56084:CDS | 50.0% | |
| TGGCCGGCAGATAGTTGATT+TGG | + | chr4.4:28077805-28077824 | MS.gene56084:CDS | 50.0% | |
| !! | CCGGCAGATAGTTGATTTGG+TGG | + | chr4.4:28077808-28077827 | MS.gene56084:CDS | 50.0% |
| GCTGCTGTTATTGCCGCTAC+TGG | - | chr4.4:28077102-28077121 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 28077092 | 28078099 | 28077092 | ID=MS.gene56084 |
| chr4.4 | mRNA | 28077092 | 28078099 | 28077092 | ID=MS.gene56084.t1;Parent=MS.gene56084 |
| chr4.4 | exon | 28077092 | 28078099 | 28077092 | ID=MS.gene56084.t1.exon1;Parent=MS.gene56084.t1 |
| chr4.4 | CDS | 28077092 | 28078099 | 28077092 | ID=cds.MS.gene56084.t1;Parent=MS.gene56084.t1 |
| Gene Sequence |
| Protein sequence |
