AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56509


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56509.t1 MTR_4g082355 92.982 285 20 0 1 285 1 285 9.90e-164 456
MS.gene56509.t1 MTR_4g082360 57.093 289 112 4 1 285 1 281 1.36e-96 286
MS.gene56509.t1 MTR_4g082365 58.397 262 86 3 1 262 1 239 5.98e-91 270
MS.gene56509.t1 MTR_4g082550 32.911 316 145 5 4 268 11 310 1.81e-30 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56509.t1 AT3G11810 34.385 317 158 7 4 274 14 326 7.00e-41 145

Find 73 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACTTGCAGATGTGGAATT+TGG 0.213824 4.2:-27366565 MS.gene56509:CDS
CCATTTCTATACTGGAATTA+AGG 0.233291 4.2:+27366516 None:intergenic
CTTGGTTATGTTATGTTTCA+TGG 0.273202 4.2:-27366902 MS.gene56509:CDS
AAACATAACATAACCAAGTT+AGG 0.281828 4.2:+27366907 None:intergenic
CTCTCTTGCATCATCGCCTT+TGG 0.286792 4.2:-27367153 MS.gene56509:CDS
TGTGGTGGTGGAATCAAGTT+GGG 0.290700 4.2:-27366809 MS.gene56509:CDS
ATCATCAACCATTTCTATAC+TGG 0.301377 4.2:+27366508 None:intergenic
TTTCTATTTGGACTTCTCAT+TGG 0.321017 4.2:-27366982 MS.gene56509:CDS
CAAGAACCCAAACAATAAAA+AGG 0.329416 4.2:+27366723 None:intergenic
GTTGAGGAGAAGTTGGAAAT+TGG 0.356766 4.2:-27366776 MS.gene56509:CDS
GATGATTGTGGTGGGGTTTG+TGG 0.365382 4.2:+27367200 None:intergenic
CCTCAAAACTAACCCTAACT+TGG 0.411518 4.2:-27366920 MS.gene56509:CDS
TTACAATATTGTGGTTAATA+CGG 0.418130 4.2:-27366620 MS.gene56509:CDS
AACTTGCAGATGTGGAATTT+GGG 0.422001 4.2:-27366564 MS.gene56509:CDS
CGGTTGCGGTGGCGAGGAGG+GGG 0.427134 4.2:+27367034 None:intergenic
AGGGCTTCTTTGAGTTTGAC+TGG 0.436290 4.2:+27367081 None:intergenic
TCGTTTCTCATCATTTACTT+AGG 0.441069 4.2:-27366952 MS.gene56509:CDS
GAGAGGAAGGAGGAAGATGA+GGG 0.451247 4.2:+27367278 None:intergenic
GGGGAAGAAGGAAGTGATGA+TGG 0.452153 4.2:+27367053 None:intergenic
TTGTGGTGGTGGAATCAAGT+TGG 0.455015 4.2:-27366810 MS.gene56509:CDS
GGGATAAGAAAGAGAGAGGA+AGG 0.458026 4.2:+27367265 None:intergenic
AGAGAGGAAGGAGGAAGATG+AGG 0.461957 4.2:+27367277 None:intergenic
AAGTGGTTACAGTTTGGTAC+TGG 0.462095 4.2:-27366680 MS.gene56509:CDS
AACGGTTGCGGTGGCGAGGA+GGG 0.464493 4.2:+27367032 None:intergenic
GCAGCTAAGTGGTTACAGTT+TGG 0.469085 4.2:-27366686 MS.gene56509:CDS
GATAGAACATTCCACATGTT+CGG 0.469357 4.2:+27367351 None:intergenic
GGAAGTGATGATGGATTTGA+GGG 0.470132 4.2:+27367062 None:intergenic
GGAAGATGAGGGAGAGAGTG+AGG 0.472871 4.2:+27367289 None:intergenic
AGGAAGTGATGATGGATTTG+AGG 0.474323 4.2:+27367061 None:intergenic
TTGAACCGTTGAGGAGAAGT+TGG 0.487160 4.2:-27366783 MS.gene56509:CDS
CTTCTTTGAGTTTGACTGGT+CGG 0.508967 4.2:+27367085 None:intergenic
TATTATGCTTTACAATATTG+TGG 0.510311 4.2:-27366629 MS.gene56509:CDS
TTTGCTAGTTAACTTGAGCT+TGG 0.511596 4.2:-27366842 MS.gene56509:CDS
AGGAGGGATAAGAAAGAGAG+AGG 0.513309 4.2:+27367261 None:intergenic
ACGGTTGCGGTGGCGAGGAG+GGG 0.520202 4.2:+27367033 None:intergenic
TGCAAGAGAGAATGATGAAG+AGG 0.520202 4.2:+27367166 None:intergenic
CAACGGTTGCGGTGGCGAGG+AGG 0.521164 4.2:+27367031 None:intergenic
AACATAACATAACCAAGTTA+GGG 0.521608 4.2:+27366908 None:intergenic
ATTCCACATGTTCGGAGCCA+TGG 0.524054 4.2:+27367359 None:intergenic
GGAAAAGTATTGGAAGAGGA+GGG 0.524529 4.2:+27367245 None:intergenic
TACAAGGAGATGATTGTGGT+GGG 0.527480 4.2:+27367192 None:intergenic
GACGACAAATGCAACGGTTG+CGG 0.532651 4.2:+27367020 None:intergenic
AGAAGTTGGAAATTGGTGAA+TGG 0.532752 4.2:-27366769 MS.gene56509:CDS
GGTGAAAATTATTGTGGTGG+TGG 0.541805 4.2:-27366821 MS.gene56509:CDS
TGGAAAAGTATTGGAAGAGG+AGG 0.541994 4.2:+27367244 None:intergenic
GATGGTGATGACAGCGCCAA+AGG 0.545392 4.2:+27367137 None:intergenic
ATGCAACGGTTGCGGTGGCG+AGG 0.548909 4.2:+27367028 None:intergenic
GGTGAATGGAATGAAAAGGT+TGG 0.549410 4.2:-27366755 MS.gene56509:CDS
GCATGGTGAACTTGCAGATG+TGG 0.558840 4.2:-27366572 MS.gene56509:CDS
GAGATACAAGGAGATGATTG+TGG 0.560857 4.2:+27367188 None:intergenic
AGCATAATAATAGCAAGTAT+AGG 0.563540 4.2:+27366643 None:intergenic
TGCAGATGTGGAATTTGGGA+AGG 0.563619 4.2:-27366560 MS.gene56509:CDS
GTGGTGGTGGAATCAAGTTG+GGG 0.564765 4.2:-27366808 MS.gene56509:CDS
ATAAGAAAGAGAGAGGAAGG+AGG 0.577919 4.2:+27367268 None:intergenic
AATTGGTGAATGGAATGAAA+AGG 0.585430 4.2:-27366759 MS.gene56509:CDS
AATGATGAAGAGGAGATACA+AGG 0.585911 4.2:+27367176 None:intergenic
ACAAGGAGATGATTGTGGTG+GGG 0.587087 4.2:+27367193 None:intergenic
GACAAATGCAACGGTTGCGG+TGG 0.587419 4.2:+27367023 None:intergenic
GTGATATTGCTGCAGCTAAG+TGG 0.587532 4.2:-27366697 MS.gene56509:CDS
GAGCTTGGTGAAAATTATTG+TGG 0.588574 4.2:-27366827 MS.gene56509:CDS
ATACCATGGCTCCGAACATG+TGG 0.598547 4.2:-27367362 None:intergenic
AATTTCCAACTTCTCCTCAA+CGG 0.599074 4.2:+27366778 None:intergenic
ACTAGCAAATACGTCACAAG+AGG 0.615825 4.2:+27366856 None:intergenic
CTGGTCGGTTAAAGAAGTCG+TGG 0.626383 4.2:+27367100 None:intergenic
CTTGGTGAAAATTATTGTGG+TGG 0.626462 4.2:-27366824 MS.gene56509:CDS
GAGAGAGTGAGGTAGTGACG+AGG 0.630169 4.2:+27367300 None:intergenic
ATACAAGGAGATGATTGTGG+TGG 0.648255 4.2:+27367191 None:intergenic
GTGGAAGACGCTGTAGGTGA+TGG 0.650170 4.2:+27367119 None:intergenic
GAAGTCGTGGAAGACGCTGT+AGG 0.656732 4.2:+27367113 None:intergenic
GAAGAAGACGACAAATGCAA+CGG 0.671477 4.2:+27367014 None:intergenic
CTAGCAAATACGTCACAAGA+GGG 0.673611 4.2:+27366857 None:intergenic
GGTGTTGTACATACACTGCA+AGG 0.675211 4.2:-27366599 MS.gene56509:CDS
ATACACTGCAAGGAGAAGCA+TGG 0.683446 4.2:-27366589 MS.gene56509:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATGCTTTACAATATTG+TGG - chr4.2:27367250-27367269 MS.gene56509:CDS 20.0%
!! TTACAATATTGTGGTTAATA+CGG - chr4.2:27367259-27367278 MS.gene56509:CDS 20.0%
!!! TTCTTCATCTTTTTTCTATT+TGG - chr4.2:27366885-27366904 MS.gene56509:CDS 20.0%
! AAACATAACATAACCAAGTT+AGG + chr4.2:27366975-27366994 None:intergenic 25.0%
! AACATAACATAACCAAGTTA+GGG + chr4.2:27366974-27366993 None:intergenic 25.0%
! AGCATAATAATAGCAAGTAT+AGG + chr4.2:27367239-27367258 None:intergenic 25.0%
!! TGTCAACCTTTTTATTGTTT+GGG - chr4.2:27367150-27367169 MS.gene56509:CDS 25.0%
!! TTGTCAACCTTTTTATTGTT+TGG - chr4.2:27367149-27367168 MS.gene56509:CDS 25.0%
!!! GAAAGTTTTTGGAAAAGTAT+TGG + chr4.2:27366647-27366666 None:intergenic 25.0%
AATTGGTGAATGGAATGAAA+AGG - chr4.2:27367120-27367139 MS.gene56509:CDS 30.0%
AATTTGGGAAGGAAAAAGAT+GGG - chr4.2:27367330-27367349 MS.gene56509:CDS 30.0%
CAAGAACCCAAACAATAAAA+AGG + chr4.2:27367159-27367178 None:intergenic 30.0%
CTTGGTTATGTTATGTTTCA+TGG - chr4.2:27366977-27366996 MS.gene56509:CDS 30.0%
TCGTTTCTCATCATTTACTT+AGG - chr4.2:27366927-27366946 MS.gene56509:CDS 30.0%
TTTCTATTTGGACTTCTCAT+TGG - chr4.2:27366897-27366916 MS.gene56509:CDS 30.0%
!! CTTTTTATTGTTTGGGTTCT+TGG - chr4.2:27367157-27367176 MS.gene56509:CDS 30.0%
!!! TTTTGGAAAAGTATTGGAAG+AGG + chr4.2:27366641-27366660 None:intergenic 30.0%
AACTTGCAGATGTGGAATTT+GGG - chr4.2:27367315-27367334 MS.gene56509:CDS 35.0%
AATGATGAAGAGGAGATACA+AGG + chr4.2:27366706-27366725 None:intergenic 35.0%
AATTTCCAACTTCTCCTCAA+CGG + chr4.2:27367104-27367123 None:intergenic 35.0%
AGAAGTTGGAAATTGGTGAA+TGG - chr4.2:27367110-27367129 MS.gene56509:CDS 35.0%
ATTTGGGAAGGAAAAAGATG+GGG - chr4.2:27367331-27367350 MS.gene56509:CDS 35.0%
CTTGGTGAAAATTATTGTGG+TGG - chr4.2:27367055-27367074 MS.gene56509:CDS 35.0%
GAATTTGGGAAGGAAAAAGA+TGG - chr4.2:27367329-27367348 MS.gene56509:CDS 35.0%
GATAGAACATTCCACATGTT+CGG + chr4.2:27366531-27366550 None:intergenic 35.0%
! GAGCTTGGTGAAAATTATTG+TGG - chr4.2:27367052-27367071 MS.gene56509:CDS 35.0%
! TTTGCTAGTTAACTTGAGCT+TGG - chr4.2:27367037-27367056 MS.gene56509:CDS 35.0%
!! GAGTTGCTGTTGAAAGTTTT+TGG + chr4.2:27366658-27366677 None:intergenic 35.0%
AAGTGGTTACAGTTTGGTAC+TGG - chr4.2:27367199-27367218 MS.gene56509:CDS 40.0%
ACTAGCAAATACGTCACAAG+AGG + chr4.2:27367026-27367045 None:intergenic 40.0%
ATAAGAAAGAGAGAGGAAGG+AGG + chr4.2:27366614-27366633 None:intergenic 40.0%
ATACAAGGAGATGATTGTGG+TGG + chr4.2:27366691-27366710 None:intergenic 40.0%
CCTCAAAACTAACCCTAACT+TGG - chr4.2:27366959-27366978 MS.gene56509:CDS 40.0%
CTAGCAAATACGTCACAAGA+GGG + chr4.2:27367025-27367044 None:intergenic 40.0%
GAACTTGCAGATGTGGAATT+TGG - chr4.2:27367314-27367333 MS.gene56509:CDS 40.0%
GAAGAAGACGACAAATGCAA+CGG + chr4.2:27366868-27366887 None:intergenic 40.0%
GAGATACAAGGAGATGATTG+TGG + chr4.2:27366694-27366713 None:intergenic 40.0%
GGTGAAAATTATTGTGGTGG+TGG - chr4.2:27367058-27367077 MS.gene56509:CDS 40.0%
GGTGAATGGAATGAAAAGGT+TGG - chr4.2:27367124-27367143 MS.gene56509:CDS 40.0%
GTTGAGGAGAAGTTGGAAAT+TGG - chr4.2:27367103-27367122 MS.gene56509:CDS 40.0%
TACAAGGAGATGATTGTGGT+GGG + chr4.2:27366690-27366709 None:intergenic 40.0%
TGCAAGAGAGAATGATGAAG+AGG + chr4.2:27366716-27366735 None:intergenic 40.0%
! CTTCTTTGAGTTTGACTGGT+CGG + chr4.2:27366797-27366816 None:intergenic 40.0%
!! AGGAAGTGATGATGGATTTG+AGG + chr4.2:27366821-27366840 None:intergenic 40.0%
!! GGAAAAGTATTGGAAGAGGA+GGG + chr4.2:27366637-27366656 None:intergenic 40.0%
!! GGAAGTGATGATGGATTTGA+GGG + chr4.2:27366820-27366839 None:intergenic 40.0%
!! TGGAAAAGTATTGGAAGAGG+AGG + chr4.2:27366638-27366657 None:intergenic 40.0%
!!! CCAAGTTAGGGTTAGTTTTG+AGG + chr4.2:27366962-27366981 None:intergenic 40.0%
!!! GGTTAGTTTTGAGGTCAACA+TGG + chr4.2:27366953-27366972 None:intergenic 40.0%
ACAAGGAGATGATTGTGGTG+GGG + chr4.2:27366689-27366708 None:intergenic 45.0%
AGGAGGGATAAGAAAGAGAG+AGG + chr4.2:27366621-27366640 None:intergenic 45.0%
ATACACTGCAAGGAGAAGCA+TGG - chr4.2:27367290-27367309 MS.gene56509:CDS 45.0%
GCAGCTAAGTGGTTACAGTT+TGG - chr4.2:27367193-27367212 MS.gene56509:CDS 45.0%
GGGATAAGAAAGAGAGAGGA+AGG + chr4.2:27366617-27366636 None:intergenic 45.0%
GTGATATTGCTGCAGCTAAG+TGG - chr4.2:27367182-27367201 MS.gene56509:CDS 45.0%
TGCAGATGTGGAATTTGGGA+AGG - chr4.2:27367319-27367338 MS.gene56509:CDS 45.0%
TTGAACCGTTGAGGAGAAGT+TGG - chr4.2:27367096-27367115 MS.gene56509:CDS 45.0%
! AGGGCTTCTTTGAGTTTGAC+TGG + chr4.2:27366801-27366820 None:intergenic 45.0%
! GGTGTTGTACATACACTGCA+AGG - chr4.2:27367280-27367299 MS.gene56509:CDS 45.0%
!! TGTGGTGGTGGAATCAAGTT+GGG - chr4.2:27367070-27367089 MS.gene56509:CDS 45.0%
!! TTGTGGTGGTGGAATCAAGT+TGG - chr4.2:27367069-27367088 MS.gene56509:CDS 45.0%
AGAGAGGAAGGAGGAAGATG+AGG + chr4.2:27366605-27366624 None:intergenic 50.0%
CTCTCTTGCATCATCGCCTT+TGG - chr4.2:27366726-27366745 MS.gene56509:CDS 50.0%
CTGGTCGGTTAAAGAAGTCG+TGG + chr4.2:27366782-27366801 None:intergenic 50.0%
GACGACAAATGCAACGGTTG+CGG + chr4.2:27366862-27366881 None:intergenic 50.0%
GAGAGGAAGGAGGAAGATGA+GGG + chr4.2:27366604-27366623 None:intergenic 50.0%
GCATGGTGAACTTGCAGATG+TGG - chr4.2:27367307-27367326 MS.gene56509:CDS 50.0%
GGGGAAGAAGGAAGTGATGA+TGG + chr4.2:27366829-27366848 None:intergenic 50.0%
! GATGATTGTGGTGGGGTTTG+TGG + chr4.2:27366682-27366701 None:intergenic 50.0%
!! GTGGTGGTGGAATCAAGTTG+GGG - chr4.2:27367071-27367090 MS.gene56509:CDS 50.0%
!!! GTTGGGGTTTTGAACCGTTG+AGG - chr4.2:27367087-27367106 MS.gene56509:CDS 50.0%
GAAGTCGTGGAAGACGCTGT+AGG + chr4.2:27366769-27366788 None:intergenic 55.0%
GACAAATGCAACGGTTGCGG+TGG + chr4.2:27366859-27366878 None:intergenic 55.0%
GAGAGAGTGAGGTAGTGACG+AGG + chr4.2:27366582-27366601 None:intergenic 55.0%
GGAAGATGAGGGAGAGAGTG+AGG + chr4.2:27366593-27366612 None:intergenic 55.0%
GTGGAAGACGCTGTAGGTGA+TGG + chr4.2:27366763-27366782 None:intergenic 55.0%
! GATGGTGATGACAGCGCCAA+AGG + chr4.2:27366745-27366764 None:intergenic 55.0%
AACGGTTGCGGTGGCGAGGA+GGG + chr4.2:27366850-27366869 None:intergenic 65.0%
ATGCAACGGTTGCGGTGGCG+AGG + chr4.2:27366854-27366873 None:intergenic 65.0%
ACGGTTGCGGTGGCGAGGAG+GGG + chr4.2:27366849-27366868 None:intergenic 70.0%
CAACGGTTGCGGTGGCGAGG+AGG + chr4.2:27366851-27366870 None:intergenic 70.0%
GGTGGCGAGGAGGGGGAAGA+AGG + chr4.2:27366841-27366860 None:intergenic 70.0%
CGGTTGCGGTGGCGAGGAGG+GGG + chr4.2:27366848-27366867 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 27366522 27367379 27366522 ID=MS.gene56509
chr4.2 mRNA 27366522 27367379 27366522 ID=MS.gene56509.t1;Parent=MS.gene56509
chr4.2 exon 27366522 27367379 27366522 ID=MS.gene56509.t1.exon1;Parent=MS.gene56509.t1
chr4.2 CDS 27366522 27367379 27366522 ID=cds.MS.gene56509.t1;Parent=MS.gene56509.t1
Gene Sequence

>MS.gene56509

ATGGCTCCGAACATGTGGAATGTTCTATCAGAATCTAAAAGCATAGTAAATGCTCAACCTCGTCACTACCTCACTCTCTCCCTCATCTTCCTCCTTCCTCTCTCTTTCTTATCCCTCCTCTTCCAATACTTTTCCAAAAACTTTCAACAGCAACTCTCCACAAACCCCACCACAATCATCTCCTTGTATCTCCTCTTCATCATTCTCTCTTGCATCATCGCCTTTGGCGCTGTCATCACCATCACCTACAGCGTCTTCCACGACTTCTTTAACCGACCAGTCAAACTCAAAGAAGCCCTCAAATCCATCATCACTTCCTTCTTCCCCCTCCTCGCCACCGCAACCGTTGCATTTGTCGTCTTCTTCTTCATCTTTTTTCTATTTGGACTTCTCATTGGACTTGTATCGTTTCTCATCATTTACTTAGGCCATGTTGACCTCAAAACTAACCCTAACTTGGTTATGTTATGTTTCATGGTACTTTTCATGATTGTTTTACTCCCTCTTGTGACGTATTTGCTAGTTAACTTGAGCTTGGTGAAAATTATTGTGGTGGTGGAATCAAGTTGGGGTTTTGAACCGTTGAGGAGAAGTTGGAAATTGGTGAATGGAATGAAAAGGTTGGTTTTGTCAACCTTTTTATTGTTTGGGTTCTTGGAAGTGATATTGCTGCAGCTAAGTGGTTACAGTTTGGTACTGGTTTTAGTGATTACTCCTATACTTGCTATTATTATGCTTTACAATATTGTGGTTAATACGGTGTTGTACATACACTGCAAGGAGAAGCATGGTGAACTTGCAGATGTGGAATTTGGGAAGGAAAAAGATGGGGCTAACTTGTCCTTAATTCCAGTATAG

Protein sequence

>MS.gene56509.t1

MAPNMWNVLSESKSIVNAQPRHYLTLSLIFLLPLSFLSLLFQYFSKNFQQQLSTNPTTIISLYLLFIILSCIIAFGAVITITYSVFHDFFNRPVKLKEALKSIITSFFPLLATATVAFVVFFFIFFLFGLLIGLVSFLIIYLGHVDLKTNPNLVMLCFMVLFMIVLLPLVTYLLVNLSLVKIIVVVESSWGFEPLRRSWKLVNGMKRLVLSTFLLFGFLEVILLQLSGYSLVLVLVITPILAIIMLYNIVVNTVLYIHCKEKHGELADVEFGKEKDGANLSLIPV