AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57670.t1 MTR_4g127420 98.571 140 1 1 1 139 184 323 1.95e-95 280
MS.gene57670.t1 MTR_2g038010 40.541 111 48 4 16 124 217 311 1.10e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57670.t1 AT5G57180 37.681 138 58 6 25 139 236 368 3.46e-14 68.6
MS.gene57670.t1 AT5G57180 37.681 138 58 6 25 139 236 368 3.46e-14 68.6
MS.gene57670.t1 AT5G57180 37.681 138 58 6 25 139 236 368 4.08e-14 68.2
MS.gene57670.t1 AT5G57180 37.681 138 58 6 25 139 236 368 4.82e-14 68.2
MS.gene57670.t1 AT4G25990 35.971 139 60 6 27 139 191 326 1.98e-12 63.5
MS.gene57670.t1 AT4G25990 35.971 139 60 6 27 139 191 326 2.36e-12 63.2
MS.gene57670.t1 AT4G25990 35.971 139 60 6 27 139 191 326 2.37e-12 63.2

Find 39 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACCGACACTGCATATTT+TGG 0.094471 8.4:+7158822 None:intergenic
TCTCATCCATGGATTGGTTT+TGG 0.279149 8.4:-7158958 MS.gene57670:CDS
ACATACATTAACATCGTTTC+CGG 0.311561 8.4:+7158600 None:intergenic
GCCATGTTGATGAAGGAAAT+TGG 0.353307 8.4:-7158882 MS.gene57670:CDS
AATTGAATTACGACGGTGTT+CGG 0.364551 8.4:-7158681 MS.gene57670:CDS
GGGAAATTCGACTTCCGGTT+AGG 0.378755 8.4:-7158934 MS.gene57670:CDS
AGGAAATTGGTGGAATTTCC+CGG 0.382166 8.4:-7158869 MS.gene57670:CDS
TCTTCTTCTCATCCATGGAT+TGG 0.411520 8.4:-7158964 MS.gene57670:CDS
GAATGCAGAATTGAAAGAAA+TGG 0.436941 8.4:-7158737 MS.gene57670:CDS
TTGGCGGGAAATTCGACTTC+CGG 0.439230 8.4:-7158939 MS.gene57670:CDS
TTTCCGGAAGCATCGGAGAC+AGG 0.440046 8.4:+7158616 None:intergenic
CACCTCTCTGTAATCCTAAC+CGG 0.443822 8.4:+7158920 None:intergenic
CATCGGCGAACGGCGTTCCT+CGG 0.459344 8.4:+7158644 None:intergenic
AGCCCTGTCTCCGATGCTTC+CGG 0.466072 8.4:-7158619 MS.gene57670:CDS
ACGTCTCTTCTTCTCATCCA+TGG 0.467379 8.4:-7158969 MS.gene57670:CDS
ACGACGGTGTTCGGAAAGCT+TGG 0.468820 8.4:-7158672 MS.gene57670:CDS
CGGTGGTAAGACGGCGGCGC+CGG 0.496178 8.4:-7158806 MS.gene57670:CDS
AACATCGTTTCCGGAAGCAT+CGG 0.505047 8.4:+7158609 None:intergenic
ATGCAGTGTCGGTGGTAAGA+CGG 0.515154 8.4:-7158815 MS.gene57670:CDS
ACCAATTTCCTTCATCAACA+TGG 0.530695 8.4:+7158881 None:intergenic
GAATCGCCAAAGTTGAAAAG+TGG 0.536005 8.4:-7158715 MS.gene57670:CDS
AAAGAAGAAGGTTGTTGCGG+TGG 0.538258 8.4:-7158767 MS.gene57670:CDS
GCTCTTCGCCATGTTGATGA+AGG 0.541149 8.4:-7158889 MS.gene57670:CDS
ATCGGAGACAGGGCTGTCAT+CGG 0.545065 8.4:+7158627 None:intergenic
TTCCCGCCAAAACCAATCCA+TGG 0.558777 8.4:+7158952 None:intergenic
CGGTTAGGATTACAGAGAGG+TGG 0.569382 8.4:-7158919 MS.gene57670:CDS
TTCCGGAAGCATCGGAGACA+GGG 0.571094 8.4:+7158617 None:intergenic
TAAGACGGCGGCGCCGGTGA+CGG 0.581889 8.4:-7158800 MS.gene57670:CDS
ACAGGGCTGTCATCGGCGAA+CGG 0.603353 8.4:+7158634 None:intergenic
CCCAAAATATGCAGTGTCGG+TGG 0.613838 8.4:-7158823 MS.gene57670:CDS
CATCATGGGAGGTAGAATTG+AGG 0.620329 8.4:-7159004 None:intergenic
TCGCCCAAAATATGCAGTGT+CGG 0.623873 8.4:-7158826 MS.gene57670:CDS
CGGAAAGCTTGGTCTGACCG+AGG 0.657705 8.4:-7158661 MS.gene57670:CDS
TTGTTGAAATTGAATTACGA+CGG 0.684346 8.4:-7158688 MS.gene57670:CDS
CAGTGTCGGTGGTAAGACGG+CGG 0.687348 8.4:-7158812 MS.gene57670:CDS
GGAATTGATAGCATCATGGG+AGG 0.697923 8.4:-7159015 None:intergenic
ATGTTGATGAAGGAAATTGG+TGG 0.707203 8.4:-7158879 MS.gene57670:CDS
TTCCGGTTAGGATTACAGAG+AGG 0.750478 8.4:-7158922 MS.gene57670:CDS
AAATTGGTGGAATTTCCCGG+CGG 0.816024 8.4:-7158866 MS.gene57670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGTTGAAATTGAATTACGA+CGG - chr8.4:7158918-7158937 MS.gene57670:CDS 25.0%
GAATGCAGAATTGAAAGAAA+TGG - chr8.4:7158869-7158888 MS.gene57670:CDS 30.0%
!! AACAATCCACTTTTCAACTT+TGG + chr8.4:7158900-7158919 None:intergenic 30.0%
AATTGAATTACGACGGTGTT+CGG - chr8.4:7158925-7158944 MS.gene57670:CDS 35.0%
ACCAATTTCCTTCATCAACA+TGG + chr8.4:7158728-7158747 None:intergenic 35.0%
GAAAAAGAAGAAGGTTGTTG+CGG - chr8.4:7158836-7158855 MS.gene57670:CDS 35.0%
GGAGAAGAAGAAAAAGAAGA+AGG - chr8.4:7158827-7158846 MS.gene57670:CDS 35.0%
! ATGTTGATGAAGGAAATTGG+TGG - chr8.4:7158727-7158746 MS.gene57670:CDS 35.0%
! ATTTTAAGCATATCAACCGC+CGG + chr8.4:7158759-7158778 None:intergenic 35.0%
AGGAAATTGGTGGAATTTCC+CGG - chr8.4:7158737-7158756 MS.gene57670:CDS 40.0%
GAATCGCCAAAGTTGAAAAG+TGG - chr8.4:7158891-7158910 MS.gene57670:CDS 40.0%
TCTTCTTCTCATCCATGGAT+TGG - chr8.4:7158642-7158661 MS.gene57670:CDS 40.0%
! GCCATGTTGATGAAGGAAAT+TGG - chr8.4:7158724-7158743 MS.gene57670:CDS 40.0%
! TTTTAAGCATATCAACCGCC+GGG + chr8.4:7158758-7158777 None:intergenic 40.0%
! TTTTTCTTCTTCTCCGTCAC+CGG + chr8.4:7158822-7158841 None:intergenic 40.0%
!!! TCTCATCCATGGATTGGTTT+TGG - chr8.4:7158648-7158667 MS.gene57670:CDS 40.0%
AAAGAAGAAGGTTGTTGCGG+TGG - chr8.4:7158839-7158858 MS.gene57670:CDS 45.0%
AAATTGGTGGAATTTCCCGG+CGG - chr8.4:7158740-7158759 MS.gene57670:CDS 45.0%
ACGTCTCTTCTTCTCATCCA+TGG - chr8.4:7158637-7158656 MS.gene57670:CDS 45.0%
CACCTCTCTGTAATCCTAAC+CGG + chr8.4:7158689-7158708 None:intergenic 45.0%
TCGCCCAAAATATGCAGTGT+CGG - chr8.4:7158780-7158799 MS.gene57670:CDS 45.0%
TTCCGGTTAGGATTACAGAG+AGG - chr8.4:7158684-7158703 MS.gene57670:CDS 45.0%
! AACATCGTTTCCGGAAGCAT+CGG + chr8.4:7159000-7159019 None:intergenic 45.0%
! ACCACCGACACTGCATATTT+TGG + chr8.4:7158787-7158806 None:intergenic 45.0%
! CCACCGACACTGCATATTTT+GGG + chr8.4:7158786-7158805 None:intergenic 45.0%
!!! ATCCATGGATTGGTTTTGGC+GGG - chr8.4:7158652-7158671 MS.gene57670:CDS 45.0%
!!! CATCCATGGATTGGTTTTGG+CGG - chr8.4:7158651-7158670 MS.gene57670:CDS 45.0%
CGGTTAGGATTACAGAGAGG+TGG - chr8.4:7158687-7158706 MS.gene57670:CDS 50.0%
GGGAAATTCGACTTCCGGTT+AGG - chr8.4:7158672-7158691 MS.gene57670:CDS 50.0%
TTCCCGCCAAAACCAATCCA+TGG + chr8.4:7158657-7158676 None:intergenic 50.0%
TTGGCGGGAAATTCGACTTC+CGG - chr8.4:7158667-7158686 MS.gene57670:CDS 50.0%
! CCCAAAATATGCAGTGTCGG+TGG - chr8.4:7158783-7158802 MS.gene57670:CDS 50.0%
! GCTCTTCGCCATGTTGATGA+AGG - chr8.4:7158717-7158736 MS.gene57670:CDS 50.0%
!! ATGCAGTGTCGGTGGTAAGA+CGG - chr8.4:7158791-7158810 MS.gene57670:CDS 50.0%
ACGACGGTGTTCGGAAAGCT+TGG - chr8.4:7158934-7158953 MS.gene57670:CDS 55.0%
ATCGGAGACAGGGCTGTCAT+CGG + chr8.4:7158982-7159001 None:intergenic 55.0%
!! TTCCGGAAGCATCGGAGACA+GGG + chr8.4:7158992-7159011 None:intergenic 55.0%
!! TTTCCGGAAGCATCGGAGAC+AGG + chr8.4:7158993-7159012 None:intergenic 55.0%
ACAGGGCTGTCATCGGCGAA+CGG + chr8.4:7158975-7158994 None:intergenic 60.0%
AGCCCTGTCTCCGATGCTTC+CGG - chr8.4:7158987-7159006 MS.gene57670:CDS 60.0%
CGGAAAGCTTGGTCTGACCG+AGG - chr8.4:7158945-7158964 MS.gene57670:CDS 60.0%
!! CAGTGTCGGTGGTAAGACGG+CGG - chr8.4:7158794-7158813 MS.gene57670:CDS 60.0%
CATCGGCGAACGGCGTTCCT+CGG + chr8.4:7158965-7158984 None:intergenic 65.0%
!! TAAGACGGCGGCGCCGGTGA+CGG - chr8.4:7158806-7158825 MS.gene57670:CDS 70.0%
CGGTGGTAAGACGGCGGCGC+CGG - chr8.4:7158800-7158819 MS.gene57670:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 7158606 7159022 7158606 ID=MS.gene57670
chr8.4 mRNA 7158606 7159022 7158606 ID=MS.gene57670.t1;Parent=MS.gene57670
chr8.4 exon 7158606 7159022 7158606 ID=MS.gene57670.t1.exon1;Parent=MS.gene57670.t1
chr8.4 CDS 7158606 7159022 7158606 ID=cds.MS.gene57670.t1;Parent=MS.gene57670.t1
Gene Sequence

>MS.gene57670

ATGGGAGGTAGAATTGAGGAGAATGAATCTAACGTCTCTTCTTCTCATCCATGGATTGGTTTTGGCGGGAAATTCGACTTCCGGTTAGGATTACAGAGAGGTGGAGTGAGAGCTCTTCGCCATGTTGATGAAGGAAATTGGTGGAATTTCCCGGCGGTTGATATGCTTAAAATTTCGCCCAAAATATGCAGTGTCGGTGGTAAGACGGCGGCGCCGGTGACGGAGAAGAAGAAAAAGAAGAAGGTTGTTGCGGTGGAGAGACTGAATGCAGAATTGAAAGAAATGGAATCGCCAAAGTTGAAAAGTGGATTGTTGTTGAAATTGAATTACGACGGTGTTCGGAAAGCTTGGTCTGACCGAGGAACGCCGTTCGCCGATGACAGCCCTGTCTCCGATGCTTCCGGAAACGATGTTAAT

Protein sequence

>MS.gene57670.t1

MGGRIEENESNVSSSHPWIGFGGKFDFRLGLQRGGVRALRHVDEGNWWNFPAVDMLKISPKICSVGGKTAAPVTEKKKKKKVVAVERLNAELKEMESPKLKSGLLLKLNYDGVRKAWSDRGTPFADDSPVSDASGNDVN