AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59838


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59838.t1 MTR_8g045300 95.541 157 7 0 1 157 1 157 2.23e-110 310
MS.gene59838.t1 MTR_8g045520 73.718 156 41 0 1 156 1 156 1.06e-84 245
MS.gene59838.t1 MTR_8g045490 68.590 156 48 1 1 156 1 155 2.97e-81 237
MS.gene59838.t1 MTR_8g045570 70.199 151 45 0 1 151 1 151 1.19e-80 235
MS.gene59838.t1 MTR_8g045400 67.308 156 50 1 1 156 1 155 1.08e-77 228
MS.gene59838.t1 MTR_8g045555 68.212 151 48 0 1 151 1 151 2.54e-77 227
MS.gene59838.t1 MTR_8g045695 65.385 156 53 1 1 156 1 155 2.74e-76 224
MS.gene59838.t1 MTR_8g045735 65.806 155 52 1 1 155 1 154 2.76e-74 219
MS.gene59838.t1 MTR_8g045640 62.821 156 57 1 1 156 1 155 1.64e-70 209
MS.gene59838.t1 MTR_8g045560 62.179 156 58 1 1 156 1 155 1.99e-69 207
MS.gene59838.t1 MTR_8g045665 61.538 156 59 1 1 156 1 155 2.85e-69 206
MS.gene59838.t1 MTR_1g030810 42.208 154 86 2 1 152 1 153 1.27e-38 129
MS.gene59838.t1 MTR_1g030820 39.610 154 90 2 1 152 1 153 4.29e-37 125
MS.gene59838.t1 MTR_8g012550 33.333 150 97 3 6 154 8 155 1.56e-26 98.2
MS.gene59838.t1 MTR_1g030840 32.692 156 97 4 1 149 1 155 4.65e-22 87.0
MS.gene59838.t1 MTR_1g031640 34.694 147 87 5 7 151 7 146 5.93e-21 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59838.t1 AT5G28010 32.895 152 99 3 3 152 15 165 7.00e-23 89.4
MS.gene59838.t1 AT5G28010 32.895 152 99 3 3 152 15 165 7.00e-23 89.4
MS.gene59838.t1 AT1G70830 32.237 152 100 3 3 152 22 172 1.23e-22 88.6
MS.gene59838.t1 AT1G70830 32.237 152 100 3 3 152 22 172 2.86e-22 88.6
MS.gene59838.t1 AT1G70840 32.237 152 100 3 3 152 20 170 6.06e-22 87.0
MS.gene59838.t1 AT1G70830 32.237 152 100 3 3 152 22 172 7.12e-22 88.6
MS.gene59838.t1 AT1G70830 32.237 152 100 3 3 152 22 172 2.07e-21 88.6
MS.gene59838.t1 AT1G70830 30.921 152 102 3 3 152 184 334 6.94e-20 84.7
MS.gene59838.t1 AT1G70890 30.921 152 102 3 3 152 7 157 2.61e-21 84.7
MS.gene59838.t1 AT1G70830 30.921 152 102 3 3 152 137 287 3.09e-20 84.7
MS.gene59838.t1 AT1G70850 30.667 150 101 3 3 150 9 157 7.53e-20 82.8
MS.gene59838.t1 AT1G70850 30.667 150 101 3 3 150 9 157 1.76e-19 83.2
MS.gene59838.t1 AT1G70850 32.394 142 93 3 13 152 175 315 1.07e-18 81.3
MS.gene59838.t1 AT1G70850 30.667 150 101 3 3 150 9 157 1.76e-19 83.2
MS.gene59838.t1 AT1G70850 32.394 142 93 3 13 152 175 315 1.07e-18 81.3
MS.gene59838.t1 AT1G23120 28.105 153 105 3 1 153 1 148 9.03e-19 78.2
MS.gene59838.t1 AT1G23120 28.571 154 103 4 2 153 50 198 4.43e-18 77.4
MS.gene59838.t1 AT1G23130 27.815 151 107 2 3 152 10 159 8.49e-18 75.9
MS.gene59838.t1 AT1G70870 32.450 151 89 4 1 151 1 138 3.98e-17 73.6
MS.gene59838.t1 AT4G23670 30.921 152 102 3 1 151 1 150 6.04e-17 73.6
MS.gene59838.t1 AT5G28000 28.947 152 105 3 3 152 13 163 7.46e-17 73.6
MS.gene59838.t1 AT1G70880 26.974 152 108 3 3 152 8 158 1.13e-16 72.8
MS.gene59838.t1 AT1G24020 29.870 154 104 3 1 152 1 152 1.27e-15 70.1
MS.gene59838.t1 AT1G24020 29.870 154 104 3 1 152 1 152 1.27e-15 70.1
MS.gene59838.t1 AT2G01520 27.632 152 107 3 1 151 1 150 2.87e-15 68.9
MS.gene59838.t1 AT1G14930 27.389 157 111 3 1 156 1 155 1.18e-14 67.4
MS.gene59838.t1 AT2G01530 28.947 152 105 3 1 151 1 150 3.28e-14 66.2
MS.gene59838.t1 AT1G35310 28.169 142 99 3 13 153 12 151 1.07e-13 65.1
MS.gene59838.t1 AT1G35260 27.815 151 99 4 7 152 6 151 1.29e-13 64.7
MS.gene59838.t1 AT4G14060 27.632 152 107 3 1 151 1 150 2.62e-13 63.9
MS.gene59838.t1 AT1G14940 27.152 151 107 3 1 150 1 149 1.66e-12 62.0
MS.gene59838.t1 AT1G30990 28.571 154 100 5 1 150 1 148 1.98e-12 61.6
MS.gene59838.t1 AT4G23680 25.000 152 111 3 1 151 1 150 2.25e-12 61.6
MS.gene59838.t1 AT1G14950 24.841 157 115 3 1 156 1 155 1.79e-11 59.3

Find 21 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAAACTTAGCACTGAAAT+TGG 0.239596 8.3:+54169528 MS.gene59838:CDS
AGGTATTCCATCCAACTATT+TGG 0.260874 8.3:-54170926 None:intergenic
ATGGCTCTACTGTAGTTAAA+TGG 0.325362 8.3:+54170864 MS.gene59838:CDS
TTCATCACCTTGATGCAGCT+TGG 0.437287 8.3:-54169637 None:intergenic
GTAAACTTAGCACTGAAATT+GGG 0.446714 8.3:+54169529 MS.gene59838:CDS
AGAAATTGATGAGCACTATA+AGG 0.468901 8.3:+54170805 MS.gene59838:CDS
AGAATCGTCCACAAGATCTT+CGG 0.493976 8.3:+54170779 MS.gene59838:CDS
TATTCCATCCAACTATTTGG+AGG 0.505271 8.3:-54170923 None:intergenic
AGCTGCATCAAGGTGATGAA+TGG 0.510022 8.3:+54169640 MS.gene59838:CDS
TTTCTTCACCGAAGATCTTG+TGG 0.512241 8.3:-54170787 None:intergenic
CATCATTGAAGTGATTGATA+AGG 0.521648 8.3:+54170838 MS.gene59838:CDS
ATGTTGAACCTCCAAATAGT+TGG 0.544310 8.3:+54170915 MS.gene59838:CDS
CGATTTCACAACAGAGGGCA+AGG 0.552186 8.3:+54170709 MS.gene59838:intron
CATGAAACCAAGCTGCATCA+AGG 0.562710 8.3:+54169630 MS.gene59838:CDS
TGAACCTCCAAATAGTTGGA+TGG 0.602941 8.3:+54170919 MS.gene59838:CDS
TGTCTCGAGTGCATTTGCTA+AGG 0.617854 8.3:-54170946 None:intergenic
ACTCTGATTCAGTTAAACAC+TGG 0.620261 8.3:+54169667 MS.gene59838:CDS
TAGTTTGAAGAACTTGTCAG+AGG 0.640528 8.3:-54169562 None:intergenic
GAAGTGATTGATAAGGCTGA+TGG 0.645628 8.3:+54170845 MS.gene59838:CDS
TCATCTTGTCAAGCAAGCAA+AGG 0.651550 8.3:+54170976 MS.gene59838:CDS
AAACACTGGACTCATGTCCG+AGG 0.721608 8.3:+54169681 MS.gene59838:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACATATTGTAAAATTT+GGG + chr8.3:54169920-54169939 MS.gene59838:intron 10.0%
!! AATTCCAATAAAAATTAAAA+CGG - chr8.3:54170680-54170699 None:intergenic 10.0%
!! ATCATCATTATATATAAAAT+TGG + chr8.3:54170400-54170419 MS.gene59838:intron 10.0%
!!! GTTTACAATTTTTTTTTTTT+TGG + chr8.3:54170515-54170534 MS.gene59838:intron 10.0%
!!! TTACAATTTTTTTTTTTTTG+GGG + chr8.3:54170517-54170536 MS.gene59838:intron 10.0%
!! ACCATTAAATTAAAATTAGA+TGG + chr8.3:54170190-54170209 MS.gene59838:intron 15.0%
!! AGTATAATTATAGAAACATA+TGG - chr8.3:54170613-54170632 None:intergenic 15.0%
!! ATTAGAAATATAATTTACCT+CGG - chr8.3:54169701-54169720 None:intergenic 15.0%
!! CAATAACATATTGTAAAATT+TGG + chr8.3:54169919-54169938 MS.gene59838:intron 15.0%
!!! AGATGAATATTTTAATTGAA+CGG + chr8.3:54170283-54170302 MS.gene59838:intron 15.0%
!!! ATAATTTAAAACTATTTGGT+TGG - chr8.3:54170472-54170491 None:intergenic 15.0%
!!! TTAACCGTTTTAATTTTTAT+TGG + chr8.3:54170673-54170692 MS.gene59838:intron 15.0%
!! AACCAATCAACAATTAATAA+TGG + chr8.3:54170110-54170129 MS.gene59838:intron 20.0%
!! ATTCAAGATCAATATCAATA+TGG - chr8.3:54170052-54170071 None:intergenic 20.0%
!! GATCTAAGTAAAAGATATAA+AGG + chr8.3:54170007-54170026 MS.gene59838:intron 20.0%
!! TAAAAATATGACAGATCTAA+AGG + chr8.3:54169971-54169990 MS.gene59838:intron 20.0%
!! TTCCATTATTAATTGTTGAT+TGG - chr8.3:54170115-54170134 None:intergenic 20.0%
!!! GACATCTTTAAATTTCATTT+TGG + chr8.3:54169850-54169869 MS.gene59838:intron 20.0%
!!! GCCATCTAATTTTAATTTAA+TGG - chr8.3:54170194-54170213 None:intergenic 20.0%
!!! TATAAAATTGGTTGATTTGA+TGG + chr8.3:54170412-54170431 MS.gene59838:intron 20.0%
!!! TTTAGATCTGTCATATTTTT+AGG - chr8.3:54169972-54169991 None:intergenic 20.0%
! ATGTACAAGTACAACTATAA+AGG + chr8.3:54170312-54170331 MS.gene59838:intron 25.0%
! ATTTCTAATAACACTAACAC+TGG + chr8.3:54169712-54169731 MS.gene59838:intron 25.0%
! GATATATATAGCAAAAGTGA+TGG + chr8.3:54170138-54170157 MS.gene59838:intron 25.0%
!!! TTGTCTTCTGATTTTTAACT+TGG + chr8.3:54169777-54169796 MS.gene59838:intron 25.0%
AGAAATTGATGAGCACTATA+AGG + chr8.3:54170805-54170824 MS.gene59838:CDS 30.0%
CATCATTGAAGTGATTGATA+AGG + chr8.3:54170838-54170857 MS.gene59838:CDS 30.0%
! GTAAACTTAGCACTGAAATT+GGG + chr8.3:54169529-54169548 MS.gene59838:CDS 30.0%
! TTGATTTGATGGTTGGAATA+GGG + chr8.3:54170423-54170442 MS.gene59838:intron 30.0%
!! AAACTATTTGGTTGGCTTTA+AGG - chr8.3:54170464-54170483 None:intergenic 30.0%
!! AAATTGGTTGATTTGATGGT+TGG + chr8.3:54170416-54170435 MS.gene59838:intron 30.0%
!! ACTGGATTTATGTGCTATTT+CGG + chr8.3:54169730-54169749 MS.gene59838:intron 30.0%
!! ATAGCAAAAGTGATGGATTT+AGG + chr8.3:54170145-54170164 MS.gene59838:intron 30.0%
!! GCTTTAAGGATCTCTAATTT+AGG - chr8.3:54170450-54170469 None:intergenic 30.0%
ACTCTGATTCAGTTAAACAC+TGG + chr8.3:54169667-54169686 MS.gene59838:CDS 35.0%
AGGTATTCCATCCAACTATT+TGG - chr8.3:54170929-54170948 None:intergenic 35.0%
ATGTTGAACCTCCAAATAGT+TGG + chr8.3:54170915-54170934 MS.gene59838:CDS 35.0%
TAGTTTGAAGAACTTGTCAG+AGG - chr8.3:54169565-54169584 None:intergenic 35.0%
TATTCCATCCAACTATTTGG+AGG - chr8.3:54170926-54170945 None:intergenic 35.0%
! ATGGCTCTACTGTAGTTAAA+TGG + chr8.3:54170864-54170883 MS.gene59838:CDS 35.0%
! GGTAAACTTAGCACTGAAAT+TGG + chr8.3:54169528-54169547 MS.gene59838:CDS 35.0%
! TGATTTGATGGTTGGAATAG+GGG + chr8.3:54170424-54170443 MS.gene59838:intron 35.0%
!! GTTGATTTGATGGTTGGAAT+AGG + chr8.3:54170422-54170441 MS.gene59838:intron 35.0%
ACCTTCGATTTCACAACAGA+GGG + chr8.3:54170704-54170723 MS.gene59838:intron 40.0%
AGAATCGTCCACAAGATCTT+CGG + chr8.3:54170779-54170798 MS.gene59838:CDS 40.0%
GAAGTGATTGATAAGGCTGA+TGG + chr8.3:54170845-54170864 MS.gene59838:CDS 40.0%
TACCTTCGATTTCACAACAG+AGG + chr8.3:54170703-54170722 MS.gene59838:intron 40.0%
TCATCTTGTCAAGCAAGCAA+AGG + chr8.3:54170976-54170995 MS.gene59838:CDS 40.0%
TGAACCTCCAAATAGTTGGA+TGG + chr8.3:54170919-54170938 MS.gene59838:CDS 40.0%
TTTCTTCACCGAAGATCTTG+TGG - chr8.3:54170790-54170809 None:intergenic 40.0%
AGCTGCATCAAGGTGATGAA+TGG + chr8.3:54169640-54169659 MS.gene59838:CDS 45.0%
CATGAAACCAAGCTGCATCA+AGG + chr8.3:54169630-54169649 MS.gene59838:CDS 45.0%
TTCATCACCTTGATGCAGCT+TGG - chr8.3:54169640-54169659 None:intergenic 45.0%
! TGTCTCGAGTGCATTTGCTA+AGG - chr8.3:54170949-54170968 None:intergenic 45.0%
!!! AATAATAATTTAAAACTATT+TGG - chr8.3:54170476-54170495 None:intergenic 5.0%
!!! TTTACAATTTTTTTTTTTTT+GGG + chr8.3:54170516-54170535 MS.gene59838:intron 5.0%
!!! TTTTTTTTTAACAAAAAATT+GGG + chr8.3:54170585-54170604 MS.gene59838:intron 5.0%
!!! TTTTTTTTTTAACAAAAAAT+TGG + chr8.3:54170584-54170603 MS.gene59838:intron 5.0%
AAACACTGGACTCATGTCCG+AGG + chr8.3:54169681-54169700 MS.gene59838:CDS 50.0%
CGATTTCACAACAGAGGGCA+AGG + chr8.3:54170709-54170728 MS.gene59838:intron 50.0%
GCCCTCTGTTGTGAAATCGA+AGG - chr8.3:54170708-54170727 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 54169516 54171009 54169516 ID=MS.gene59838
chr8.3 mRNA 54169516 54171009 54169516 ID=MS.gene59838.t1;Parent=MS.gene59838
chr8.3 exon 54169516 54169702 54169516 ID=MS.gene59838.t1.exon1;Parent=MS.gene59838.t1
chr8.3 CDS 54169516 54169702 54169516 ID=cds.MS.gene59838.t1;Parent=MS.gene59838.t1
chr8.3 exon 54170723 54171009 54170723 ID=MS.gene59838.t1.exon2;Parent=MS.gene59838.t1
chr8.3 CDS 54170723 54171009 54170723 ID=cds.MS.gene59838.t1;Parent=MS.gene59838.t1
Gene Sequence

>MS.gene59838

ATGGTGCTTGCTGGTAAACTTAGCACTGAAATTGGGATCAAAGCATCCTCTGACAAGTTCTTCAAACTATTCGCATCAAATATTCACGAACTGCAAAACATTTGTGAAAGTGTTCATGAAACCAAGCTGCATCAAGGTGATGAATGGCATCACTCTGATTCAGTTAAACACTGGACTCATGTCCGAGGTAAATTATATTTCTAATAACACTAACACTGGATTTATGTGCTATTTCGGTCTATCAATAAATTTATAATTTATTTGTCTTCTGATTTTTAACTTGGCTAAATAAAATTTATTTCTCATATTCTTCGAAAAAAATATATTTCTCATAGACATCTTTAAATTTCATTTTGGCAATAACATATTATAAAAAGTTTAATATAACGTAAAAAATAGATGACAATAACATATTGTAAAATTTGGGATATAATTTTTAAAAGAAGATTTAGTCCTAAAAATATGACAGATCTAAAGGTTATATTATGACAGATCTAAGTAAAAGATATAAAGGTTCAAATTGTATAAATATACCATATTGATATTGATCTTGAATTTTGTATAAAACAAACATTTCTCAAGATTTGTTAACAAAACCAATCAACAATTAATAATGGAACAAGATATATATAGCAAAAGTGATGGATTTAGGTTTCAAATAGATGTCATTACTGACCATTAAATTAAAATTAGATGGCTCACGTATCAATTGAAGAGTTTTTGTCAACATACACAATTAATTAAACTCTGAGTCGTTTGATCAAAATAGATGAATATTTTAATTGAACGGTTTGTTATGTACAAGTACAACTATAAAGGATTCTTCTTATACTATAGAAACATAATACATACAGTACATTATATGTCAAAATCAATGATAAATAATCATCATTATATATAAAATTGGTTGATTTGATGGTTGGAATAGGGGCCTAAATTAGAGATCCTTAAAGCCAACCAAATAGTTTTAAATTATTATTATTCATTCAGTTCGATTTTGTTTACAATTTTTTTTTTTTTGGGGAACAACATATTTTATTTAAGAAATGAAAACAATATATTATTAACTTTTTTTTTTAACAAAAAATTGGGTTCCATATGTTTCTATAATTATACTTTCATAATTATTCTTTCAAAAAAATGTTTATATAATTATTTTAACCGTTTTAATTTTTATTGGAATTAATTACCTTCGATTTCACAACAGAGGGCAAGGTATACACATGTCATGAGAGTTTCGAAGAAATTGATGAAAAGAACAAAAGAATCGTCCACAAGATCTTCGGTGAAGAAATTGATGAGCACTATAAGGTCTTTAAGCTCATCATTGAAGTGATTGATAAGGCTGATGGCTCTACTGTAGTTAAATGGACTGTTGAATATGAGAAGATCAATGAAGATGTTGAACCTCCAAATAGTTGGATGGAATACCTTAGCAAATGCACTCGAGACATTGATGCTCATCTTGTCAAGCAAGCAAAGGTTGCTCCATAA

Protein sequence

>MS.gene59838.t1

MVLAGKLSTEIGIKASSDKFFKLFASNIHELQNICESVHETKLHQGDEWHHSDSVKHWTHVREGKVYTCHESFEEIDEKNKRIVHKIFGEEIDEHYKVFKLIIEVIDKADGSTVVKWTVEYEKINEDVEPPNSWMEYLSKCTRDIDAHLVKQAKVAP