AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61228


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61228.t1 MTR_2g102227 97.628 253 5 1 1 252 244 496 0.0 509
MS.gene61228.t1 MTR_6g486270 52.252 222 101 3 30 248 129 348 1.54e-62 200
MS.gene61228.t1 MTR_8g022290 28.758 306 141 10 10 247 37 333 3.44e-21 91.7
MS.gene61228.t1 MTR_4g079960 43.617 94 47 2 31 118 31 124 5.18e-18 82.0
MS.gene61228.t1 MTR_4g079960 40.541 74 44 0 176 249 220 293 4.26e-11 62.4
MS.gene61228.t1 MTR_4g079960 43.158 95 48 2 31 119 31 125 5.96e-18 81.6
MS.gene61228.t1 MTR_4g079960 40.541 74 44 0 176 249 220 293 5.30e-11 62.0
MS.gene61228.t1 MTR_4g079960 43.158 95 48 2 31 119 31 125 7.80e-18 81.6
MS.gene61228.t1 MTR_4g079960 40.541 74 44 0 176 249 220 293 5.16e-11 62.0
MS.gene61228.t1 MTR_2g016140 39.785 93 51 2 31 118 23 115 2.70e-17 80.1
MS.gene61228.t1 MTR_2g016140 39.785 93 51 2 31 118 23 115 3.05e-17 80.1
MS.gene61228.t1 MTR_2g092960 31.250 112 65 2 11 118 34 137 5.95e-12 65.9
MS.gene61228.t1 MTR_1g037650 25.850 147 100 3 30 172 37 178 1.58e-11 62.0
MS.gene61228.t1 MTR_7g081190 31.579 95 57 2 31 117 46 140 8.08e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61228.t1 AT3G24490 43.083 253 124 4 1 252 73 306 1.86e-60 194
MS.gene61228.t1 AT3G14180 42.453 106 54 3 31 133 84 185 1.64e-23 98.6
MS.gene61228.t1 AT3G11100 33.182 220 137 3 31 246 22 235 5.97e-21 89.0
MS.gene61228.t1 AT1G54060 31.439 264 137 8 31 252 94 355 5.94e-19 85.5
MS.gene61228.t1 AT5G05550 28.814 236 129 5 31 250 24 236 1.21e-17 80.1
MS.gene61228.t1 AT5G05550 28.814 236 129 5 31 250 24 236 1.60e-17 79.7
MS.gene61228.t1 AT5G05550 28.814 236 129 5 31 250 24 236 1.94e-17 79.3
MS.gene61228.t1 AT3G58630 37.255 102 50 2 31 118 26 127 1.75e-16 77.8
MS.gene61228.t1 AT3G58630 37.255 102 50 2 31 118 26 127 1.96e-16 77.8
MS.gene61228.t1 AT3G10030 34.783 92 59 1 30 120 160 251 2.32e-14 72.4
MS.gene61228.t1 AT3G10030 34.783 92 59 1 30 120 160 251 2.58e-14 72.4
MS.gene61228.t1 AT3G24860 30.400 125 77 3 16 134 50 170 4.57e-13 68.2

Find 51 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGATTCCATTCAGAATTT+TGG 0.118439 2.1:-3318646 MS.gene61228:CDS
CAAGATTAAGTTTCAAGATA+TGG 0.238032 2.1:-3318944 MS.gene61228:CDS
GCAGCTGCTAAACGATCTTT+TGG 0.284124 2.1:-3319180 MS.gene61228:CDS
TGGGTGGTGGAGATAGAAAA+TGG 0.290828 2.1:-3318924 MS.gene61228:CDS
CATTTGTTCTTCTTGATGCT+TGG 0.296454 2.1:-3319119 MS.gene61228:CDS
ATTTGTTCTTCTTGATGCTT+GGG 0.319429 2.1:-3319118 MS.gene61228:CDS
AGAAGATTGAAAATAATAAA+AGG 0.319723 2.1:-3318612 MS.gene61228:CDS
ACAGAATCGGTTCGTGTAGA+AGG 0.331232 2.1:-3318742 MS.gene61228:CDS
GATGGATTTCCTCAACGATT+TGG 0.369450 2.1:-3318560 MS.gene61228:CDS
TTCTTGATGCTTGGGGTGAT+CGG 0.409344 2.1:-3319110 MS.gene61228:CDS
ATCGGTTTCTGCAACATAGA+AGG 0.423435 2.1:-3319092 MS.gene61228:CDS
AAGATTAAGTTTCAAGATAT+GGG 0.423794 2.1:-3318943 MS.gene61228:CDS
AACTTATGAATTTGCAGCTC+GGG 0.427130 2.1:-3319220 MS.gene61228:CDS
ATGGCAACAAGTTGCAGAAA+AGG 0.453580 2.1:-3319049 MS.gene61228:CDS
TAATAAAAGGCAGCAGATGA+TGG 0.461717 2.1:-3318599 MS.gene61228:CDS
GTTTCAAAAGTTGAAAGAAC+GGG 0.463936 2.1:-3319018 MS.gene61228:CDS
GGGTGGTGGAGATAGAAAAT+GGG 0.465027 2.1:-3318923 MS.gene61228:CDS
CCTTCTCAGTCCATTCAGTA+AGG 0.479775 2.1:+3319145 None:intergenic
GCAGCTGCAGCTGACGGTGT+TGG 0.482446 2.1:+3319195 None:intergenic
TTAGGAATTCCCTTACTGAA+TGG 0.483595 2.1:-3319155 MS.gene61228:CDS
GGAATCAGCAAGTAATTTGA+AGG 0.488399 2.1:+3318660 None:intergenic
TATCTTACCAAAATTCTGAA+TGG 0.497261 2.1:+3318639 None:intergenic
TCCTCCTGAAAATACAGAAT+CGG 0.498451 2.1:-3318755 MS.gene61228:CDS
GCATGATTTACATAAACTCC+AGG 0.504047 2.1:+3318805 None:intergenic
CAAAGAAAAGAAGGAAAGGA+AGG 0.514012 2.1:-3318702 MS.gene61228:CDS
GGCCTTGCCCAAAGAAAAGA+AGG 0.520654 2.1:-3318711 MS.gene61228:CDS
AAGTTTCAAGATATGGGTGG+TGG 0.524195 2.1:-3318937 MS.gene61228:CDS
TCTCTTGAGTTCGAACGACA+AGG 0.531751 2.1:+3318844 None:intergenic
GGAACTAGAAAAGATGAGGA+TGG 0.544427 2.1:-3318578 MS.gene61228:CDS
GGGAGTCTTGCTTGTTGTGG+TGG 0.550724 2.1:+3318865 None:intergenic
AAAGAAAAGAAGGAAAGGAA+GGG 0.551393 2.1:-3318701 MS.gene61228:CDS
TTTGTTCTTCTTGATGCTTG+GGG 0.554775 2.1:-3319117 MS.gene61228:CDS
AAGAAAAGAAGGAAAGGAAG+GGG 0.556042 2.1:-3318700 MS.gene61228:CDS
GTTCGTGTAGAAGGTTTCGA+AGG 0.557020 2.1:-3318733 MS.gene61228:CDS
CGTTTAGCAGCTGCAGCTGA+CGG 0.562335 2.1:+3319189 None:intergenic
CTCTTGAGTTCGAACGACAA+GGG 0.566224 2.1:+3318845 None:intergenic
GGTTTCAAAAGTTGAAAGAA+CGG 0.571460 2.1:-3319019 MS.gene61228:CDS
TGTAAATCATGCAAATGAGA+TGG 0.574988 2.1:-3318794 MS.gene61228:CDS
AAGGAAGAGTCTTCGATCTG+AGG 0.584495 2.1:-3319073 MS.gene61228:CDS
TGCCCAAAGAAAAGAAGGAA+AGG 0.586775 2.1:-3318706 MS.gene61228:CDS
CAACTTATGAATTTGCAGCT+CGG 0.587886 2.1:-3319221 MS.gene61228:CDS
CAAGGGAGTCTTGCTTGTTG+TGG 0.589582 2.1:+3318862 None:intergenic
CTTCTCAGTCCATTCAGTAA+GGG 0.589672 2.1:+3319146 None:intergenic
AGAGTCTTCGATCTGAGGAA+TGG 0.593143 2.1:-3319068 MS.gene61228:CDS
AGTTGCAGAAAAGGTATCAG+TGG 0.596635 2.1:-3319040 MS.gene61228:CDS
TTCGTGTAGAAGGTTTCGAA+GGG 0.597829 2.1:-3318732 MS.gene61228:CDS
CCTTACTGAATGGACTGAGA+AGG 0.603468 2.1:-3319145 MS.gene61228:CDS
TGATGGAACTAGAAAAGATG+AGG 0.616160 2.1:-3318582 MS.gene61228:CDS
AAATGAGATGGATGACATGA+AGG 0.616691 2.1:-3318782 MS.gene61228:CDS
ATTAAGTTTCAAGATATGGG+TGG 0.645987 2.1:-3318940 MS.gene61228:CDS
TTTGTGCTTCCAAATCGTTG+AGG 0.661381 2.1:+3318551 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAGATTGAAAATAATAAA+AGG - chr2.1:3319102-3319121 MS.gene61228:CDS 15.0%
!! ATTGAAGAAAAAATACAAAA+AGG - chr2.1:3318746-3318765 MS.gene61228:CDS 15.0%
!! AAGATTAAGTTTCAAGATAT+GGG - chr2.1:3318771-3318790 MS.gene61228:CDS 20.0%
! CAAGATTAAGTTTCAAGATA+TGG - chr2.1:3318770-3318789 MS.gene61228:CDS 25.0%
! TATCTTACCAAAATTCTGAA+TGG + chr2.1:3319078-3319097 None:intergenic 25.0%
!!! ATGGGTTTATTTTCAAAAGA+TGG - chr2.1:3318809-3318828 MS.gene61228:CDS 25.0%
AAAGAAAAGAAGGAAAGGAA+GGG - chr2.1:3319013-3319032 MS.gene61228:CDS 30.0%
ATTAAGTTTCAAGATATGGG+TGG - chr2.1:3318774-3318793 MS.gene61228:CDS 30.0%
ATTTGTTCTTCTTGATGCTT+GGG - chr2.1:3318596-3318615 MS.gene61228:CDS 30.0%
GGTTTCAAAAGTTGAAAGAA+CGG - chr2.1:3318695-3318714 MS.gene61228:CDS 30.0%
GTTTCAAAAGTTGAAAGAAC+GGG - chr2.1:3318696-3318715 MS.gene61228:CDS 30.0%
TGTAAATCATGCAAATGAGA+TGG - chr2.1:3318920-3318939 MS.gene61228:CDS 30.0%
! TTCCTTTCCTTCTTTTCTTT+GGG + chr2.1:3319013-3319032 None:intergenic 30.0%
AAATGAGATGGATGACATGA+AGG - chr2.1:3318932-3318951 MS.gene61228:CDS 35.0%
AACTTATGAATTTGCAGCTC+GGG - chr2.1:3318494-3318513 MS.gene61228:CDS 35.0%
AAGAAAAGAAGGAAAGGAAG+GGG - chr2.1:3319014-3319033 MS.gene61228:CDS 35.0%
CAAAGAAAAGAAGGAAAGGA+AGG - chr2.1:3319012-3319031 MS.gene61228:CDS 35.0%
CAACTTATGAATTTGCAGCT+CGG - chr2.1:3318493-3318512 MS.gene61228:CDS 35.0%
CATTTGTTCTTCTTGATGCT+TGG - chr2.1:3318595-3318614 MS.gene61228:CDS 35.0%
GCATGATTTACATAAACTCC+AGG + chr2.1:3318912-3318931 None:intergenic 35.0%
GGAATCAGCAAGTAATTTGA+AGG + chr2.1:3319057-3319076 None:intergenic 35.0%
TAATAAAAGGCAGCAGATGA+TGG - chr2.1:3319115-3319134 MS.gene61228:CDS 35.0%
TCCTCCTGAAAATACAGAAT+CGG - chr2.1:3318959-3318978 MS.gene61228:CDS 35.0%
TGATGGAACTAGAAAAGATG+AGG - chr2.1:3319132-3319151 MS.gene61228:CDS 35.0%
TTAGGAATTCCCTTACTGAA+TGG - chr2.1:3318559-3318578 MS.gene61228:CDS 35.0%
TTTGTTCTTCTTGATGCTTG+GGG - chr2.1:3318597-3318616 MS.gene61228:CDS 35.0%
! CTTCCTTTCCTTCTTTTCTT+TGG + chr2.1:3319014-3319033 None:intergenic 35.0%
!! GCTGATTCCATTCAGAATTT+TGG - chr2.1:3319068-3319087 MS.gene61228:CDS 35.0%
AAGTTTCAAGATATGGGTGG+TGG - chr2.1:3318777-3318796 MS.gene61228:CDS 40.0%
ATGGCAACAAGTTGCAGAAA+AGG - chr2.1:3318665-3318684 MS.gene61228:CDS 40.0%
CTTCTCAGTCCATTCAGTAA+GGG + chr2.1:3318571-3318590 None:intergenic 40.0%
GGAACTAGAAAAGATGAGGA+TGG - chr2.1:3319136-3319155 MS.gene61228:CDS 40.0%
TGCCCAAAGAAAAGAAGGAA+AGG - chr2.1:3319008-3319027 MS.gene61228:CDS 40.0%
TTCGTGTAGAAGGTTTCGAA+GGG - chr2.1:3318982-3319001 MS.gene61228:CDS 40.0%
TTTGTGCTTCCAAATCGTTG+AGG + chr2.1:3319166-3319185 None:intergenic 40.0%
! AGCTGCTAAACGATCTTTTG+GGG - chr2.1:3318536-3318555 MS.gene61228:CDS 40.0%
! ATCGGTTTCTGCAACATAGA+AGG - chr2.1:3318622-3318641 MS.gene61228:CDS 40.0%
! CAGCTGCTAAACGATCTTTT+GGG - chr2.1:3318535-3318554 MS.gene61228:CDS 40.0%
! GATGGATTTCCTCAACGATT+TGG - chr2.1:3319154-3319173 MS.gene61228:CDS 40.0%
! TTCCTTCTTTTCTTTGGGCA+AGG + chr2.1:3319008-3319027 None:intergenic 40.0%
!! ACCGATTCTGTATTTTCAGG+AGG + chr2.1:3318963-3318982 None:intergenic 40.0%
!! AGTTGCAGAAAAGGTATCAG+TGG - chr2.1:3318674-3318693 MS.gene61228:CDS 40.0%
!! CGAACCGATTCTGTATTTTC+AGG + chr2.1:3318966-3318985 None:intergenic 40.0%
!! GAGCGTATTTTTATGAAGCC+TGG - chr2.1:3318891-3318910 MS.gene61228:CDS 40.0%
!!! CTAAACGATCTTTTGGGGTT+AGG - chr2.1:3318541-3318560 MS.gene61228:CDS 40.0%
AAGGAAGAGTCTTCGATCTG+AGG - chr2.1:3318641-3318660 MS.gene61228:CDS 45.0%
AGAGTCTTCGATCTGAGGAA+TGG - chr2.1:3318646-3318665 MS.gene61228:CDS 45.0%
CCTTCTCAGTCCATTCAGTA+AGG + chr2.1:3318572-3318591 None:intergenic 45.0%
CTCTTGAGTTCGAACGACAA+GGG + chr2.1:3318872-3318891 None:intergenic 45.0%
GTTCGTGTAGAAGGTTTCGA+AGG - chr2.1:3318981-3319000 MS.gene61228:CDS 45.0%
TCTCTTGAGTTCGAACGACA+AGG + chr2.1:3318873-3318892 None:intergenic 45.0%
TTCTTGATGCTTGGGGTGAT+CGG - chr2.1:3318604-3318623 MS.gene61228:CDS 45.0%
! GCAGCTGCTAAACGATCTTT+TGG - chr2.1:3318534-3318553 MS.gene61228:CDS 45.0%
!! ACAGAATCGGTTCGTGTAGA+AGG - chr2.1:3318972-3318991 MS.gene61228:CDS 45.0%
!! CCTTACTGAATGGACTGAGA+AGG - chr2.1:3318569-3318588 MS.gene61228:CDS 45.0%
!! GGGTGGTGGAGATAGAAAAT+GGG - chr2.1:3318791-3318810 MS.gene61228:CDS 45.0%
!! TGGGTGGTGGAGATAGAAAA+TGG - chr2.1:3318790-3318809 MS.gene61228:CDS 45.0%
GGCCTTGCCCAAAGAAAAGA+AGG - chr2.1:3319003-3319022 MS.gene61228:CDS 50.0%
! CAAGGGAGTCTTGCTTGTTG+TGG + chr2.1:3318855-3318874 None:intergenic 50.0%
CGTTTAGCAGCTGCAGCTGA+CGG + chr2.1:3318528-3318547 None:intergenic 55.0%
!! GGGAGTCTTGCTTGTTGTGG+TGG + chr2.1:3318852-3318871 None:intergenic 55.0%
GCAGCTGCAGCTGACGGTGT+TGG + chr2.1:3318522-3318541 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 3318489 3319247 3318489 ID=MS.gene61228
chr2.1 mRNA 3318489 3319247 3318489 ID=MS.gene61228.t1;Parent=MS.gene61228
chr2.1 exon 3318489 3319247 3318489 ID=MS.gene61228.t1.exon1;Parent=MS.gene61228.t1
chr2.1 CDS 3318489 3319247 3318489 ID=cds.MS.gene61228.t1;Parent=MS.gene61228.t1
Gene Sequence

>MS.gene61228

ATGTCAACTTATGAATTTGCAGCTCGGGTGCCAACACCGTCAGCTGCAGCTGCTAAACGATCTTTTGGGGTTAGGAATTCCCTTACTGAATGGACTGAGAAGGAGACATTTGTTCTTCTTGATGCTTGGGGTGATCGGTTTCTGCAACATAGAAGGAAGAGTCTTCGATCTGAGGAATGGCAACAAGTTGCAGAAAAGGTATCAGTGGTTTCAAAAGTTGAAAGAACGGGTACTCAGTGTAGAAATCGTTTAGATACATTGAAGAAAAAATACAAAAAGGACAAGATTAAGTTTCAAGATATGGGTGGTGGAGATAGAAAATGGGTTTATTTTCAAAAGATGGATAAACTAATGTCTTCTCCACCACAACAAGCAAGACTCCCTTGTCGTTCGAACTCAAGAGAGCGTATTTTTATGAAGCCTGGAGTTTATGTAAATCATGCAAATGAGATGGATGACATGAAGGATAGTCCTCCTGAAAATACAGAATCGGTTCGTGTAGAAGGTTTCGAAGGGCCTTGCCCAAAGAAAAGAAGGAAAGGAAGGGGAAGTGATGAGTTTTCTTCCTTCAAATTACTTGCTGATTCCATTCAGAATTTTGGTAAGATATATGAGAAGATTGAAAATAATAAAAGGCAGCAGATGATGGAACTAGAAAAGATGAGGATGGATTTCCTCAACGATTTGGAAGCACAAAAGAGACAGATTTTTGAAAGATTGCAGAGTGAGATTTTAAAAATCGAGCAAAGAGATGATTAA

Protein sequence

>MS.gene61228.t1

MSTYEFAARVPTPSAAAAKRSFGVRNSLTEWTEKETFVLLDAWGDRFLQHRRKSLRSEEWQQVAEKVSVVSKVERTGTQCRNRLDTLKKKYKKDKIKFQDMGGGDRKWVYFQKMDKLMSSPPQQARLPCRSNSRERIFMKPGVYVNHANEMDDMKDSPPENTESVRVEGFEGPCPKKRRKGRGSDEFSSFKLLADSIQNFGKIYEKIENNKRQQMMELEKMRMDFLNDLEAQKRQIFERLQSEILKIEQRDD