AlfalfaGEDB | MS.gene68392

M. sativa cultivar XinJiangDaYe / MS.gene68392

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68392.t1	XP_003590175.2	78.6	145	31	0	1	145	152	296	7.10E-52	213.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68392.t1	G7I6P2	78.6	145	31	0	1	145	152	296	5.1e-52	213.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene68392	0.854051	1.59E-61	-1.69E-46
MS.gene049171	MS.gene68392	0.864738	9.80E-65	-1.69E-46
MS.gene049222	MS.gene68392	0.806762	7.03E-50	-1.69E-46
MS.gene049285	MS.gene68392	0.803836	2.88E-49	-1.69E-46
MS.gene049427	MS.gene68392	0.808624	2.83E-50	-1.69E-46
MS.gene049609	MS.gene68392	0.832452	9.38E-56	-1.69E-46
MS.gene049633	MS.gene68392	0.801205	1.01E-48	-1.69E-46
MS.gene049805	MS.gene68392	0.857011	2.18E-62	-1.69E-46
MS.gene050577	MS.gene68392	0.806396	8.39E-50	-1.69E-46
MS.gene050593	MS.gene68392	0.815913	7.24E-52	-1.69E-46
MS.gene050747	MS.gene68392	0.845349	4.29E-59	-1.69E-46
MS.gene050772	MS.gene68392	0.816823	4.53E-52	-1.69E-46
MS.gene05081	MS.gene68392	0.808101	3.65E-50	-1.69E-46
MS.gene050950	MS.gene68392	0.837863	4.04E-57	-1.69E-46
MS.gene051666	MS.gene68392	0.853285	2.64E-61	-1.69E-46
MS.gene051838	MS.gene68392	0.819292	1.25E-52	-1.69E-46
MS.gene052322	MS.gene68392	0.837295	5.66E-57	-1.69E-46
MS.gene052481	MS.gene68392	0.842606	2.33E-58	-1.69E-46
MS.gene052484	MS.gene68392	0.803589	3.24E-49	-1.69E-46
MS.gene052501	MS.gene68392	0.827372	1.62E-54	-1.69E-46
MS.gene052503	MS.gene68392	0.812415	4.29E-51	-1.69E-46
MS.gene052505	MS.gene68392	0.82543	4.71E-54	-1.69E-46
MS.gene052606	MS.gene68392	-0.811388	7.18E-51	-1.69E-46
MS.gene052615	MS.gene68392	0.811673	6.22E-51	-1.69E-46
MS.gene052823	MS.gene68392	0.806644	7.44E-50	-1.69E-46
MS.gene052825	MS.gene68392	0.804956	1.68E-49	-1.69E-46
MS.gene052982	MS.gene68392	0.813488	2.50E-51	-1.69E-46
MS.gene053103	MS.gene68392	0.829286	5.61E-55	-1.69E-46
MS.gene053104	MS.gene68392	0.801572	8.45E-49	-1.69E-46
MS.gene053203	MS.gene68392	0.810672	1.03E-50	-1.69E-46
MS.gene053322	MS.gene68392	0.802667	5.03E-49	-1.69E-46
MS.gene053579	MS.gene68392	0.818583	1.82E-52	-1.69E-46
MS.gene054408	MS.gene68392	0.824089	9.75E-54	-1.69E-46
MS.gene054642	MS.gene68392	0.801618	8.27E-49	-1.69E-46
MS.gene05470	MS.gene68392	0.815682	8.16E-52	-1.69E-46
MS.gene054884	MS.gene68392	-0.827584	1.44E-54	-1.69E-46
MS.gene055206	MS.gene68392	0.83251	9.07E-56	-1.69E-46
MS.gene055289	MS.gene68392	0.81074	9.92E-51	-1.69E-46
MS.gene055362	MS.gene68392	0.804176	2.45E-49	-1.69E-46
MS.gene055502	MS.gene68392	0.803716	3.05E-49	-1.69E-46
MS.gene055536	MS.gene68392	-0.823526	1.32E-53	-1.69E-46
MS.gene055748	MS.gene68392	0.81643	5.55E-52	-1.69E-46
MS.gene056692	MS.gene68392	0.81261	3.89E-51	-1.69E-46
MS.gene056897	MS.gene68392	0.800984	1.11E-48	-1.69E-46
MS.gene057124	MS.gene68392	0.803751	3.00E-49	-1.69E-46
MS.gene057196	MS.gene68392	0.804984	1.66E-49	-1.69E-46
MS.gene057331	MS.gene68392	0.803142	4.01E-49	-1.69E-46
MS.gene057332	MS.gene68392	0.80784	4.15E-50	-1.69E-46
MS.gene057416	MS.gene68392	0.847463	1.14E-59	-1.69E-46
MS.gene057658	MS.gene68392	0.840771	7.11E-58	-1.69E-46
MS.gene058419	MS.gene68392	0.822983	1.77E-53	-1.69E-46
MS.gene058544	MS.gene68392	0.860992	1.40E-63	-1.69E-46
MS.gene058838	MS.gene68392	0.874547	6.20E-68	-1.69E-46
MS.gene059023	MS.gene68392	0.832633	8.46E-56	-1.69E-46
MS.gene05914	MS.gene68392	0.808832	2.55E-50	-1.69E-46
MS.gene059536	MS.gene68392	0.82591	3.62E-54	-1.69E-46
MS.gene059677	MS.gene68392	0.802156	6.41E-49	-1.69E-46
MS.gene059718	MS.gene68392	0.80452	2.08E-49	-1.69E-46
MS.gene059960	MS.gene68392	0.805557	1.26E-49	-1.69E-46
MS.gene060403	MS.gene68392	0.828735	7.62E-55	-1.69E-46
MS.gene060762	MS.gene68392	0.819138	1.36E-52	-1.69E-46
MS.gene06092	MS.gene68392	0.849947	2.33E-60	-1.69E-46
MS.gene061150	MS.gene68392	0.867917	9.61E-66	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68392.t1	MTR_1g045520	94.483	145	8	0	1	145	152	296	2.37e-84	249
MS.gene68392.t1	MTR_4g088590	65.060	83	5	1	1	83	74	132	1.63e-16	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68392.t1	AT3G04310	35.948	153	81	4	1	145	125	268	3.54e-20	84.0
MS.gene68392.t1	AT3G04310	35.948	153	81	4	1	145	125	268	3.54e-20	84.0

Find 39 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTGGCAATTGAGAAATTA+TGG	0.159956	1.3:+27129563	MS.gene68392:CDS
AGCCGAGATGCACCAAGTTT+TGG	0.334550	1.3:+27129754	MS.gene68392:CDS
TTCATCCTATACTTGCGCTT+TGG	0.407380	1.3:-27129604	None:intergenic
AGCGAGCTAGGCCAACAAAA+AGG	0.417127	1.3:+27129794	MS.gene68392:CDS
CCTGTGTGTCATTTACATTT+CGG	0.419738	1.3:-27129636	None:intergenic
CTTGCGCTTTGGTTTGCTCT+CGG	0.443826	1.3:-27129593	None:intergenic
ACTGGCAATTGAGAAATTAT+GGG	0.445083	1.3:+27129564	MS.gene68392:CDS
AGGGTCACAAAGGATGAATA+TGG	0.454789	1.3:+27129834	MS.gene68392:CDS
GCAAACCAAAGCGCAAGTAT+AGG	0.470582	1.3:+27129599	MS.gene68392:CDS
TGGAAGGTGGTAGGTTGAGT+AGG	0.473865	1.3:+27129854	MS.gene68392:CDS
AAAACTAATACGAGGAAGAC+GGG	0.477410	1.3:+27129685	MS.gene68392:CDS
TCATCCCATCCACCAAAACT+TGG	0.498543	1.3:-27129766	None:intergenic
GGGATGACTTGGAGCGAGCT+AGG	0.499950	1.3:+27129782	MS.gene68392:CDS
TGTAAATGACACACAGGTTG+AGG	0.502592	1.3:+27129642	MS.gene68392:CDS
CACCAAAACTTGGTGCATCT+CGG	0.507741	1.3:-27129756	None:intergenic
CAGTCAGTCAGCACTTGCAC+TGG	0.515720	1.3:+27129546	MS.gene68392:CDS
GGAGATCACAAGCAAGGAAA+GGG	0.523252	1.3:+27129815	MS.gene68392:CDS
GGAAGACGGGGATGCAGTCG+TGG	0.532326	1.3:+27129698	MS.gene68392:CDS
AACAACGGTTCGGTTGATAG+TGG	0.539590	1.3:+27129730	MS.gene68392:CDS
AGGAGATCACAAGCAAGGAA+AGG	0.557690	1.3:+27129814	MS.gene68392:CDS
AATAAGCAATCGAACCAAGA+GGG	0.559725	1.3:-27129899	None:intergenic
GGTAGTTGAGAACAACGGTT+CGG	0.582840	1.3:+27129720	MS.gene68392:CDS
GGATGAATATGGAAGGTGGT+AGG	0.584534	1.3:+27129845	MS.gene68392:CDS
AGCGCAAGTATAGGATGAAG+AGG	0.587300	1.3:+27129608	MS.gene68392:CDS
GAAAACTAATACGAGGAAGA+CGG	0.592704	1.3:+27129684	MS.gene68392:CDS
CAATTGAGAAATTATGGGGA+CGG	0.615205	1.3:+27129569	MS.gene68392:CDS
CAAAGGATGAATATGGAAGG+TGG	0.642176	1.3:+27129841	MS.gene68392:CDS
AAGCAAGGAAAGGGTCACAA+AGG	0.643486	1.3:+27129824	MS.gene68392:CDS
GAAGACGGGGATGCAGTCGT+GGG	0.644899	1.3:+27129699	MS.gene68392:CDS
TCACAAAGGATGAATATGGA+AGG	0.645284	1.3:+27129838	MS.gene68392:CDS
ATAAGCAATCGAACCAAGAG+GGG	0.649272	1.3:-27129898	None:intergenic
CTGGCAATTGAGAAATTATG+GGG	0.651354	1.3:+27129565	MS.gene68392:CDS
GCAAGTGCTGACTGACTGAG+AGG	0.659866	1.3:-27129541	None:intergenic
CAAGTGCTGACTGACTGAGA+GGG	0.679661	1.3:-27129540	None:intergenic
CAATAAGCAATCGAACCAAG+AGG	0.681035	1.3:-27129900	None:intergenic
CCGAAATGTAAATGACACAC+AGG	0.683264	1.3:+27129636	MS.gene68392:CDS
AAACTAATACGAGGAAGACG+GGG	0.698039	1.3:+27129686	MS.gene68392:CDS
AAACCCAGAAAACTAATACG+AGG	0.734217	1.3:+27129677	MS.gene68392:CDS
TCGTGGGTAGTTGAGAACAA+CGG	0.734928	1.3:+27129715	MS.gene68392:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTGCTATTTTTCCATTTTT+GGG	+	chr1.3:27129919-27129938	MS.gene68392:CDS	20.0%
!!!	TATTGCTATTTTTCCATTTT+TGG	+	chr1.3:27129918-27129937	MS.gene68392:CDS	20.0%
	ACTGGCAATTGAGAAATTAT+GGG	+	chr1.3:27129564-27129583	MS.gene68392:CDS	30.0%
	AAAACTAATACGAGGAAGAC+GGG	+	chr1.3:27129685-27129704	MS.gene68392:CDS	35.0%
	AAACCCAGAAAACTAATACG+AGG	+	chr1.3:27129677-27129696	MS.gene68392:CDS	35.0%
	AATAAGCAATCGAACCAAGA+GGG	-	chr1.3:27129902-27129921	None:intergenic	35.0%
	CAATTGAGAAATTATGGGGA+CGG	+	chr1.3:27129569-27129588	MS.gene68392:CDS	35.0%
	CACTGGCAATTGAGAAATTA+TGG	+	chr1.3:27129563-27129582	MS.gene68392:CDS	35.0%
	CTGGCAATTGAGAAATTATG+GGG	+	chr1.3:27129565-27129584	MS.gene68392:CDS	35.0%
	GAAAACTAATACGAGGAAGA+CGG	+	chr1.3:27129684-27129703	MS.gene68392:CDS	35.0%
	TCACAAAGGATGAATATGGA+AGG	+	chr1.3:27129838-27129857	MS.gene68392:CDS	35.0%
!!	CCTGTGTGTCATTTACATTT+CGG	-	chr1.3:27129639-27129658	None:intergenic	35.0%
!!	TTAGTTTTCTGGGTTTCTTC+TGG	-	chr1.3:27129673-27129692	None:intergenic	35.0%
!!	TTTTTCCATTTTTGGGCACA+TGG	+	chr1.3:27129926-27129945	MS.gene68392:CDS	35.0%
!!!	CTTCCTCGTATTAGTTTTCT+GGG	-	chr1.3:27129683-27129702	None:intergenic	35.0%
!!!	TCTTCCTCGTATTAGTTTTC+TGG	-	chr1.3:27129684-27129703	None:intergenic	35.0%
	AAACTAATACGAGGAAGACG+GGG	+	chr1.3:27129686-27129705	MS.gene68392:CDS	40.0%
	AGGGTCACAAAGGATGAATA+TGG	+	chr1.3:27129834-27129853	MS.gene68392:CDS	40.0%
	ATAAGCAATCGAACCAAGAG+GGG	-	chr1.3:27129901-27129920	None:intergenic	40.0%
	CAAAAAGGAGATCACAAGCA+AGG	+	chr1.3:27129809-27129828	MS.gene68392:CDS	40.0%
	CAAAGGATGAATATGGAAGG+TGG	+	chr1.3:27129841-27129860	MS.gene68392:CDS	40.0%
	CAATAAGCAATCGAACCAAG+AGG	-	chr1.3:27129903-27129922	None:intergenic	40.0%
	CCGAAATGTAAATGACACAC+AGG	+	chr1.3:27129636-27129655	MS.gene68392:CDS	40.0%
	TGTAAATGACACACAGGTTG+AGG	+	chr1.3:27129642-27129661	MS.gene68392:CDS	40.0%
	TTCATCCTATACTTGCGCTT+TGG	-	chr1.3:27129607-27129626	None:intergenic	40.0%
	TTTGTCCATGTGCCCAAAAA+TGG	-	chr1.3:27129934-27129953	None:intergenic	40.0%
!	GCTTGTGATCTCCTTTTTGT+TGG	-	chr1.3:27129808-27129827	None:intergenic	40.0%
	AAAAAGCGATACTCCCCTCT+TGG	+	chr1.3:27129885-27129904	MS.gene68392:CDS	45.0%
	AAGCAAGGAAAGGGTCACAA+AGG	+	chr1.3:27129824-27129843	MS.gene68392:CDS	45.0%
	AGCGCAAGTATAGGATGAAG+AGG	+	chr1.3:27129608-27129627	MS.gene68392:CDS	45.0%
	AGGAGATCACAAGCAAGGAA+AGG	+	chr1.3:27129814-27129833	MS.gene68392:CDS	45.0%
	GCAAACCAAAGCGCAAGTAT+AGG	+	chr1.3:27129599-27129618	MS.gene68392:CDS	45.0%
	GGAGATCACAAGCAAGGAAA+GGG	+	chr1.3:27129815-27129834	MS.gene68392:CDS	45.0%
	GGATGAATATGGAAGGTGGT+AGG	+	chr1.3:27129845-27129864	MS.gene68392:CDS	45.0%
	GGTAGTTGAGAACAACGGTT+CGG	+	chr1.3:27129720-27129739	MS.gene68392:CDS	45.0%
	TCATCCCATCCACCAAAACT+TGG	-	chr1.3:27129769-27129788	None:intergenic	45.0%
	TCGTGGGTAGTTGAGAACAA+CGG	+	chr1.3:27129715-27129734	MS.gene68392:CDS	45.0%
!!	AACAACGGTTCGGTTGATAG+TGG	+	chr1.3:27129730-27129749	MS.gene68392:CDS	45.0%
!!	ATGCACCAAGTTTTGGTGGA+TGG	+	chr1.3:27129761-27129780	MS.gene68392:CDS	45.0%
!!	CACCAAAACTTGGTGCATCT+CGG	-	chr1.3:27129759-27129778	None:intergenic	45.0%
!!	TGCACCAAGTTTTGGTGGAT+GGG	+	chr1.3:27129762-27129781	MS.gene68392:CDS	45.0%
!!	TTTTGGTGGATGGGATGACT+TGG	+	chr1.3:27129771-27129790	MS.gene68392:CDS	45.0%
	AGCGAGCTAGGCCAACAAAA+AGG	+	chr1.3:27129794-27129813	MS.gene68392:CDS	50.0%
!	AGCCGAGATGCACCAAGTTT+TGG	+	chr1.3:27129754-27129773	MS.gene68392:CDS	50.0%
!	TGGAAGGTGGTAGGTTGAGT+AGG	+	chr1.3:27129854-27129873	MS.gene68392:CDS	50.0%
!!	CAAGTGCTGACTGACTGAGA+GGG	-	chr1.3:27129543-27129562	None:intergenic	50.0%
!!	CGAGATGCACCAAGTTTTGG+TGG	+	chr1.3:27129757-27129776	MS.gene68392:CDS	50.0%
!!	CTTGCGCTTTGGTTTGCTCT+CGG	-	chr1.3:27129596-27129615	None:intergenic	50.0%
	CAGTCAGTCAGCACTTGCAC+TGG	+	chr1.3:27129546-27129565	MS.gene68392:CDS	55.0%
!!	GCAAGTGCTGACTGACTGAG+AGG	-	chr1.3:27129544-27129563	None:intergenic	55.0%
	GAAGACGGGGATGCAGTCGT+GGG	+	chr1.3:27129699-27129718	MS.gene68392:CDS	60.0%
!	GGGATGACTTGGAGCGAGCT+AGG	+	chr1.3:27129782-27129801	MS.gene68392:CDS	60.0%
	GGAAGACGGGGATGCAGTCG+TGG	+	chr1.3:27129698-27129717	MS.gene68392:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	27129526	27129963	27129526	ID=MS.gene68392
chr1.3	mRNA	27129526	27129963	27129526	ID=MS.gene68392.t1;Parent=MS.gene68392
chr1.3	exon	27129526	27129963	27129526	ID=MS.gene68392.t1.exon1;Parent=MS.gene68392.t1
chr1.3	CDS	27129526	27129963	27129526	ID=cds.MS.gene68392.t1;Parent=MS.gene68392.t1

Gene Sequence

>MS.gene68392

ATGGCATTAGCAATCCCTCTCAGTCAGTCAGCACTTGCACTGGCAATTGAGAAATTATGGGGACGGACCGAGAGCAAACCAAAGCGCAAGTATAGGATGAAGAGGAAACGCCGAAATGTAAATGACACACAGGTTGAGGAAGAGCCAGAAGAAACCCAGAAAACTAATACGAGGAAGACGGGGATGCAGTCGTGGGTAGTTGAGAACAACGGTTCGGTTGATAGTGGCAGCCGAGATGCACCAAGTTTTGGTGGATGGGATGACTTGGAGCGAGCTAGGCCAACAAAAAGGAGATCACAAGCAAGGAAAGGGTCACAAAGGATGAATATGGAAGGTGGTAGGTTGAGTAGGAGAGAAAGAAAAAGCGATACTCCCCTCTTGGTTCGATTGCTTATTGCTATTTTTCCATTTTTGGGCACATGGACAAAGATGCTGTAA

Protein sequence

>MS.gene68392.t1

MALAIPLSQSALALAIEKLWGRTESKPKRKYRMKRKRRNVNDTQVEEEPEETQKTNTRKTGMQSWVVENNGSVDSGSRDAPSFGGWDDLERARPTKRRSQARKGSQRMNMEGGRLSRRERKSDTPLLVRLLIAIFPFLGTWTKML