Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77929.t1 | XP_003599257.1 | 89.6 | 106 | 11 | 0 | 1 | 106 | 1 | 106 | 3.30E-43 | 184.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77929.t1 | P31336 | 52.3 | 107 | 48 | 2 | 1 | 106 | 1 | 105 | 2.2e-19 | 96.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77929.t1 | G7IWU5 | 89.6 | 106 | 11 | 0 | 1 | 106 | 1 | 106 | 2.4e-43 | 184.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene77929 | 0.818591 | 1.81E-52 | -1.69E-46 |
| MS.gene049173 | MS.gene77929 | 0.831452 | 1.66E-55 | -1.69E-46 |
| MS.gene049217 | MS.gene77929 | 0.808586 | 2.88E-50 | -1.69E-46 |
| MS.gene049222 | MS.gene77929 | 0.804215 | 2.40E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene77929 | 0.862119 | 6.35E-64 | -1.69E-46 |
| MS.gene049320 | MS.gene77929 | 0.857807 | 1.27E-62 | -1.69E-46 |
| MS.gene04939 | MS.gene77929 | 0.843138 | 1.68E-58 | -1.69E-46 |
| MS.gene049614 | MS.gene77929 | 0.809316 | 2.01E-50 | -1.69E-46 |
| MS.gene050163 | MS.gene77929 | 0.869609 | 2.72E-66 | -1.69E-46 |
| MS.gene050165 | MS.gene77929 | 0.863966 | 1.71E-64 | -1.69E-46 |
| MS.gene050333 | MS.gene77929 | 0.826317 | 2.90E-54 | -1.69E-46 |
| MS.gene050504 | MS.gene77929 | 0.834445 | 2.98E-56 | -1.69E-46 |
| MS.gene050518 | MS.gene77929 | 0.808437 | 3.10E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene77929 | 0.845925 | 3.00E-59 | -1.69E-46 |
| MS.gene050675 | MS.gene77929 | 0.802611 | 5.17E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene77929 | 0.829105 | 6.20E-55 | -1.69E-46 |
| MS.gene050767 | MS.gene77929 | 0.801573 | 8.45E-49 | -1.69E-46 |
| MS.gene050770 | MS.gene77929 | 0.803281 | 3.76E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene77929 | 0.817248 | 3.64E-52 | -1.69E-46 |
| MS.gene050986 | MS.gene77929 | 0.846316 | 2.34E-59 | -1.69E-46 |
| MS.gene05100 | MS.gene77929 | 0.858833 | 6.28E-63 | -1.69E-46 |
| MS.gene051228 | MS.gene77929 | 0.829305 | 5.54E-55 | -1.69E-46 |
| MS.gene051551 | MS.gene77929 | 0.82861 | 8.17E-55 | -1.69E-46 |
| MS.gene051810 | MS.gene77929 | 0.895665 | 7.73E-76 | -1.69E-46 |
| MS.gene051949 | MS.gene77929 | 0.812528 | 4.05E-51 | -1.69E-46 |
| MS.gene052271 | MS.gene77929 | 0.842384 | 2.67E-58 | -1.69E-46 |
| MS.gene052379 | MS.gene77929 | 0.823124 | 1.64E-53 | -1.69E-46 |
| MS.gene052615 | MS.gene77929 | 0.821721 | 3.47E-53 | -1.69E-46 |
| MS.gene053207 | MS.gene77929 | 0.804074 | 2.57E-49 | -1.69E-46 |
| MS.gene053213 | MS.gene77929 | 0.842821 | 2.05E-58 | -1.69E-46 |
| MS.gene053286 | MS.gene77929 | 0.841 | 6.19E-58 | -1.69E-46 |
| MS.gene053322 | MS.gene77929 | 0.810153 | 1.33E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene77929 | 0.827808 | 1.27E-54 | -1.69E-46 |
| MS.gene054600 | MS.gene77929 | 0.844224 | 8.63E-59 | -1.69E-46 |
| MS.gene054642 | MS.gene77929 | 0.856968 | 2.25E-62 | -1.69E-46 |
| MS.gene054954 | MS.gene77929 | 0.842291 | 2.83E-58 | -1.69E-46 |
| MS.gene055212 | MS.gene77929 | 0.810521 | 1.11E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene77929 | 0.856809 | 2.50E-62 | -1.69E-46 |
| MS.gene055397 | MS.gene77929 | 0.813603 | 2.35E-51 | -1.69E-46 |
| MS.gene055499 | MS.gene77929 | 0.838969 | 2.10E-57 | -1.69E-46 |
| MS.gene055502 | MS.gene77929 | 0.825011 | 5.92E-54 | -1.69E-46 |
| MS.gene055722 | MS.gene77929 | 0.805398 | 1.36E-49 | -1.69E-46 |
| MS.gene055776 | MS.gene77929 | 0.808356 | 3.22E-50 | -1.69E-46 |
| MS.gene056374 | MS.gene77929 | 0.852324 | 4.97E-61 | -1.69E-46 |
| MS.gene056504 | MS.gene77929 | 0.808151 | 3.57E-50 | -1.69E-46 |
| MS.gene056510 | MS.gene77929 | 0.809368 | 1.96E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene77929 | 0.864896 | 8.74E-65 | -1.69E-46 |
| MS.gene057129 | MS.gene77929 | 0.839349 | 1.67E-57 | -1.69E-46 |
| MS.gene057246 | MS.gene77929 | 0.802076 | 6.66E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene77929 | 0.807988 | 3.86E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene77929 | 0.842249 | 2.90E-58 | -1.69E-46 |
| MS.gene058544 | MS.gene77929 | 0.815422 | 9.32E-52 | -1.69E-46 |
| MS.gene058723 | MS.gene77929 | 0.836594 | 8.54E-57 | -1.69E-46 |
| MS.gene058773 | MS.gene77929 | 0.831074 | 2.05E-55 | -1.69E-46 |
| MS.gene059022 | MS.gene77929 | 0.869904 | 2.18E-66 | -1.69E-46 |
| MS.gene059029 | MS.gene77929 | 0.809068 | 2.27E-50 | -1.69E-46 |
| MS.gene059677 | MS.gene77929 | 0.872786 | 2.43E-67 | -1.69E-46 |
| MS.gene059718 | MS.gene77929 | 0.832756 | 7.88E-56 | -1.69E-46 |
| MS.gene059858 | MS.gene77929 | 0.851303 | 9.69E-61 | -1.69E-46 |
| MS.gene059865 | MS.gene77929 | 0.817809 | 2.72E-52 | -1.69E-46 |
| MS.gene060341 | MS.gene77929 | 0.809835 | 1.55E-50 | -1.69E-46 |
| MS.gene060703 | MS.gene77929 | 0.826169 | 3.14E-54 | -1.69E-46 |
| MS.gene060762 | MS.gene77929 | 0.838849 | 2.25E-57 | -1.69E-46 |
| MS.gene06077 | MS.gene77929 | 0.807483 | 4.95E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene77929 | 0.833222 | 6.04E-56 | -1.69E-46 |
| MS.gene061200 | MS.gene77929 | 0.800827 | 1.20E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77929.t1 | MTR_3g030850 | 89.623 | 106 | 11 | 0 | 1 | 106 | 1 | 106 | 2.29e-63 | 187 |
| MS.gene77929.t1 | MTR_3g030860 | 80.952 | 105 | 19 | 1 | 1 | 105 | 1 | 104 | 3.75e-48 | 149 |
| MS.gene77929.t1 | MTR_4g037505 | 68.224 | 107 | 32 | 2 | 1 | 105 | 1 | 107 | 3.97e-44 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77929.t1 | AT1G51400 | 54.630 | 108 | 45 | 2 | 1 | 106 | 1 | 106 | 2.67e-27 | 96.7 |
| MS.gene77929.t1 | AT3G21055 | 52.336 | 107 | 44 | 4 | 1 | 105 | 1 | 102 | 2.11e-21 | 81.6 |
| MS.gene77929.t1 | AT3G21055 | 52.336 | 107 | 44 | 4 | 1 | 105 | 41 | 142 | 5.09e-21 | 81.6 |
Find 24 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCTCAATCCTCGGCTTCT+CGG | 0.406107 | 3.2:-21579921 | MS.gene77929:CDS |
| TGACGACGAGGAGTCTCCTC+TGG | 0.437430 | 3.2:+21579870 | None:intergenic |
| AATGCTGTCCGAGCAGTTGA+TGG | 0.443602 | 3.2:-21579848 | MS.gene77929:CDS |
| GTTGGCATAGTGACACAAAC+AGG | 0.491066 | 3.2:+21579665 | None:intergenic |
| GCTTCTCGGCTGTAACCAAC+CGG | 0.513686 | 3.2:-21579907 | MS.gene77929:CDS |
| ATGCTGTCCGAGCAGTTGAT+GGG | 0.515597 | 3.2:-21579847 | MS.gene77929:CDS |
| TCTGGGATGCTACGGATGAC+CGG | 0.520845 | 3.2:+21579888 | None:intergenic |
| AGCCGAGAAGCCGAGGATTG+AGG | 0.533092 | 3.2:+21579919 | None:intergenic |
| GAGTCTCCTCTGGGATGCTA+CGG | 0.538143 | 3.2:+21579880 | None:intergenic |
| GCCGAGGATTGAGGATGTCA+TGG | 0.565591 | 3.2:+21579928 | None:intergenic |
| GACGACGAGGAGTCTCCTCT+GGG | 0.572413 | 3.2:+21579871 | None:intergenic |
| ACCATGACATCCTCAATCCT+CGG | 0.573002 | 3.2:-21579929 | MS.gene77929:CDS |
| AACAACAAAGAGGGAAGCAA+CGG | 0.574516 | 3.2:-21579803 | MS.gene77929:CDS |
| GTGTCACTATGCCAACAGCT+AGG | 0.576409 | 3.2:-21579658 | MS.gene77929:CDS |
| TTGCGACAAATCCTAGCTGT+TGG | 0.581063 | 3.2:+21579647 | None:intergenic |
| GGATGCTACGGATGACCGGT+TGG | 0.593143 | 3.2:+21579892 | None:intergenic |
| TGGTTACAGCCGAGAAGCCG+AGG | 0.594889 | 3.2:+21579912 | None:intergenic |
| CAAGTATGACAACAACAAAG+AGG | 0.600137 | 3.2:-21579813 | MS.gene77929:CDS |
| TGCTGTCCGAGCAGTTGATG+GGG | 0.601918 | 3.2:-21579846 | MS.gene77929:CDS |
| ACAAAGAGGGAAGCAACGGA+AGG | 0.609771 | 3.2:-21579799 | MS.gene77929:CDS |
| GCAGATGATGAACCTAAAAG+AGG | 0.625646 | 3.2:-21579716 | MS.gene77929:CDS |
| CTCGGACAGCATTGACGACG+AGG | 0.627380 | 3.2:+21579858 | None:intergenic |
| AAGTATGACAACAACAAAGA+GGG | 0.629437 | 3.2:-21579812 | MS.gene77929:CDS |
| GGTCATCCGTAGCATCCCAG+AGG | 0.774161 | 3.2:-21579886 | MS.gene77929:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGTATGACAACAACAAAGA+GGG | - | chr3.2:21579772-21579791 | MS.gene77929:CDS | 30.0% | |
| !!! | ATACTTTTTCTTTGCTTCTG+GGG | + | chr3.2:21579896-21579915 | None:intergenic | 30.0% |
| !!! | CATACTTTTTCTTTGCTTCT+GGG | + | chr3.2:21579897-21579916 | None:intergenic | 30.0% |
| CAAGTATGACAACAACAAAG+AGG | - | chr3.2:21579771-21579790 | MS.gene77929:CDS | 35.0% | |
| !!! | GCATACTTTTTCTTTGCTTC+TGG | + | chr3.2:21579898-21579917 | None:intergenic | 35.0% |
| AACAACAAAGAGGGAAGCAA+CGG | - | chr3.2:21579781-21579800 | MS.gene77929:CDS | 40.0% | |
| GCAGATGATGAACCTAAAAG+AGG | - | chr3.2:21579868-21579887 | MS.gene77929:CDS | 40.0% | |
| ACCATGACATCCTCAATCCT+CGG | - | chr3.2:21579655-21579674 | MS.gene77929:CDS | 45.0% | |
| GTTGGCATAGTGACACAAAC+AGG | + | chr3.2:21579922-21579941 | None:intergenic | 45.0% | |
| TTGCGACAAATCCTAGCTGT+TGG | + | chr3.2:21579940-21579959 | None:intergenic | 45.0% | |
| ! | GCTTCTGTTTGCACTTTTGC+TGG | - | chr3.2:21579835-21579854 | MS.gene77929:CDS | 45.0% |
| ! | TCTTTTCCCCATCAACTGCT+CGG | + | chr3.2:21579747-21579766 | None:intergenic | 45.0% |
| !!! | CACTTTTGCTGGTGTTGCAA+CGG | - | chr3.2:21579846-21579865 | MS.gene77929:CDS | 45.0% |
| AATGCTGTCCGAGCAGTTGA+TGG | - | chr3.2:21579736-21579755 | MS.gene77929:CDS | 50.0% | |
| ACAAAGAGGGAAGCAACGGA+AGG | - | chr3.2:21579785-21579804 | MS.gene77929:CDS | 50.0% | |
| ATCCTCAATCCTCGGCTTCT+CGG | - | chr3.2:21579663-21579682 | MS.gene77929:CDS | 50.0% | |
| ATGCTGTCCGAGCAGTTGAT+GGG | - | chr3.2:21579737-21579756 | MS.gene77929:CDS | 50.0% | |
| ! | GTGTCACTATGCCAACAGCT+AGG | - | chr3.2:21579926-21579945 | MS.gene77929:CDS | 50.0% |
| !!! | GCTTCTGGGGTACCTCTTTT+AGG | + | chr3.2:21579883-21579902 | None:intergenic | 50.0% |
| GCCGAGGATTGAGGATGTCA+TGG | + | chr3.2:21579659-21579678 | None:intergenic | 55.0% | |
| GCTTCTCGGCTGTAACCAAC+CGG | - | chr3.2:21579677-21579696 | MS.gene77929:CDS | 55.0% | |
| ! | TCTGGGATGCTACGGATGAC+CGG | + | chr3.2:21579699-21579718 | None:intergenic | 55.0% |
| ! | TGCTGTCCGAGCAGTTGATG+GGG | - | chr3.2:21579738-21579757 | MS.gene77929:CDS | 55.0% |
| !! | GAGTCTCCTCTGGGATGCTA+CGG | + | chr3.2:21579707-21579726 | None:intergenic | 55.0% |
| AGCCGAGAAGCCGAGGATTG+AGG | + | chr3.2:21579668-21579687 | None:intergenic | 60.0% | |
| GACGACGAGGAGTCTCCTCT+GGG | + | chr3.2:21579716-21579735 | None:intergenic | 60.0% | |
| GGATGCTACGGATGACCGGT+TGG | + | chr3.2:21579695-21579714 | None:intergenic | 60.0% | |
| GGTCATCCGTAGCATCCCAG+AGG | - | chr3.2:21579698-21579717 | MS.gene77929:CDS | 60.0% | |
| TGACGACGAGGAGTCTCCTC+TGG | + | chr3.2:21579717-21579736 | None:intergenic | 60.0% | |
| TGGTTACAGCCGAGAAGCCG+AGG | + | chr3.2:21579675-21579694 | None:intergenic | 60.0% | |
| !! | CTCGGACAGCATTGACGACG+AGG | + | chr3.2:21579729-21579748 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 21579643 | 21579963 | 21579643 | ID=MS.gene77929 |
| chr3.2 | mRNA | 21579643 | 21579963 | 21579643 | ID=MS.gene77929.t1;Parent=MS.gene77929 |
| chr3.2 | exon | 21579643 | 21579963 | 21579643 | ID=MS.gene77929.t1.exon1;Parent=MS.gene77929.t1 |
| chr3.2 | CDS | 21579643 | 21579963 | 21579643 | ID=cds.MS.gene77929.t1;Parent=MS.gene77929.t1 |
| Gene Sequence |
| Protein sequence |
