AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78773


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78773.t1 MTR_5g049230 99.603 252 1 0 1 252 47 298 0.0 509
MS.gene78773.t1 MTR_5g049240 86.508 252 34 0 1 252 2 253 1.04e-160 449
MS.gene78773.t1 MTR_5g049240 86.508 252 34 0 1 252 47 298 1.47e-159 447
MS.gene78773.t1 MTR_8g069850 76.984 252 58 0 1 252 47 298 3.06e-147 414
MS.gene78773.t1 MTR_4g086150 44.017 234 124 2 19 252 61 287 2.54e-64 204
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78773.t1 AT1G13580 69.841 252 76 0 1 252 47 298 1.03e-130 372
MS.gene78773.t1 AT1G13580 69.841 252 76 0 1 252 47 298 1.03e-130 372
MS.gene78773.t1 AT3G25540 63.922 255 88 2 1 252 47 300 2.49e-118 340
MS.gene78773.t1 AT3G25540 63.922 255 88 2 1 252 47 300 2.49e-118 340
MS.gene78773.t1 AT1G26200 56.870 262 97 3 1 252 46 301 3.54e-104 305
MS.gene78773.t1 AT1G26200 54.444 270 102 3 1 252 46 312 2.67e-101 298
MS.gene78773.t1 AT1G13580 71.354 192 55 0 1 192 47 238 1.34e-99 290
MS.gene78773.t1 AT3G25540 64.615 195 66 1 1 192 47 241 2.03e-90 267
MS.gene78773.t1 AT1G26200 60.309 194 74 1 1 194 46 236 6.60e-82 245
MS.gene78773.t1 AT3G19260 45.528 246 127 2 7 252 51 289 9.46e-73 224

Find 37 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAAGATTAACAAATTTA+AGG 0.125028 5.1:+40799568 MS.gene78773:CDS
CCATACTTGCTTTAATATTC+TGG 0.136312 5.1:+40799878 MS.gene78773:CDS
TGCTTGTTCTTAACATTTAT+TGG 0.179322 5.1:+40801044 MS.gene78773:CDS
TGATGCTACTGATGTGTTTC+TGG 0.182167 5.1:+40800189 MS.gene78773:CDS
GTGTCGTTTACTACCCATTT+TGG 0.238501 5.1:+40800299 MS.gene78773:CDS
CCAGAATATTAAAGCAAGTA+TGG 0.250823 5.1:-40799878 None:intergenic
ACTGGTTGCCACCTTGAAAT+TGG 0.253838 5.1:+40800961 MS.gene78773:CDS
ACACTCAGGTTTGTTCGAAT+TGG 0.325304 5.1:+40800145 MS.gene78773:intron
GAAACAAGACGCTCTGACTT+TGG 0.337924 5.1:+40799901 MS.gene78773:CDS
GTACTTCGAAGAACCCAAAA+TGG 0.344972 5.1:-40800313 None:intergenic
CTGATTCTTCGGATGCTTGT+TGG 0.351437 5.1:+40801073 MS.gene78773:CDS
CCTTGAAATTGGAAAATCAT+TGG 0.362965 5.1:+40800972 MS.gene78773:CDS
AAACAAGACGCTCTGACTTT+GGG 0.369876 5.1:+40799902 MS.gene78773:CDS
CATACTTGCTTTAATATTCT+GGG 0.380439 5.1:+40799879 MS.gene78773:CDS
TTGGAAAATCATTGGGTGAA+TGG 0.396407 5.1:+40800980 MS.gene78773:CDS
ACTGATGTGTTTCTGGAGAT+AGG 0.414778 5.1:+40800196 MS.gene78773:CDS
CTTGAAATTGGAAAATCATT+GGG 0.421846 5.1:+40800973 MS.gene78773:CDS
ATTGGTGGGTGCTGATTCTT+CGG 0.444061 5.1:+40801062 MS.gene78773:CDS
TCTGGCCATCTCTGATTCCC+TGG 0.444917 5.1:-40799695 None:intergenic
TCTTTGTTGCAGCTATGAAC+TGG 0.456363 5.1:+40800943 MS.gene78773:intron
CTGATGTGTTTCTGGAGATA+GGG 0.490323 5.1:+40800197 MS.gene78773:CDS
CTGTGACATATGACGAGCCT+TGG 0.495300 5.1:+40799642 MS.gene78773:CDS
TTTGGGTTCTTCGAAGTACA+AGG 0.496763 5.1:+40800317 MS.gene78773:CDS
TTGTTCTTAACATTTATTGG+TGG 0.510641 5.1:+40801047 MS.gene78773:CDS
GTTAGTGAAGATATACGATC+TGG 0.513493 5.1:+40801118 MS.gene78773:CDS
GTAGTAAACGACACGTAGTA+TGG 0.517462 5.1:-40800290 None:intergenic
GGGCTGTATATGTATGGTGC+TGG 0.528738 5.1:+40799841 MS.gene78773:CDS
TGTTCTTAACATTTATTGGT+GGG 0.530572 5.1:+40801048 MS.gene78773:CDS
TTGAAAGGGCTGTATATGTA+TGG 0.545225 5.1:+40799835 MS.gene78773:CDS
TACTTCGAAGAACCCAAAAT+GGG 0.551224 5.1:-40800312 None:intergenic
TTGGGCCAGGGAATCAGAGA+TGG 0.559128 5.1:+40799690 MS.gene78773:CDS
GTTGGTCAAATTCGAGCAAG+AGG 0.587622 5.1:+40801091 MS.gene78773:CDS
TTGGTCAAATTCGAGCAAGA+GGG 0.591303 5.1:+40801092 MS.gene78773:CDS
ATGTGTAGAAATTGGCCAGA+CGG 0.612314 5.1:+40799492 None:intergenic
AAATGTAGGATAACACAATG+AGG 0.666787 5.1:-40799957 None:intergenic
GGGAAAATGTCCAAATACAG+CGG 0.695232 5.1:+40800217 MS.gene78773:CDS
ACAAATTTAAGGAATCAGCG+TGG 0.724000 5.1:+40799579 MS.gene78773:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAAAATTAAGTAAAAT+AGG - chr5.1:40800125-40800144 None:intergenic 10.0%
!! AAAGAAGATTAACAAATTTA+AGG + chr5.1:40799568-40799587 MS.gene78773:CDS 15.0%
!! AATAAATTACTCAGTTTATA+CGG + chr5.1:40800885-40800904 MS.gene78773:intron 15.0%
!! ATATCCTTTAAATTTACATT+TGG - chr5.1:40800454-40800473 None:intergenic 15.0%
!! TATCCTTTAAATTTACATTT+GGG - chr5.1:40800453-40800472 None:intergenic 15.0%
!!! TACTGATACAAAATATTTTT+GGG + chr5.1:40799667-40799686 MS.gene78773:CDS 15.0%
!!! TTACTGATACAAAATATTTT+TGG + chr5.1:40799666-40799685 MS.gene78773:CDS 15.0%
!! AATCCCAAATGTAAATTTAA+AGG + chr5.1:40800447-40800466 MS.gene78773:intron 20.0%
!! TATATGAGTAAAGTATAAAG+TGG + chr5.1:40800752-40800771 MS.gene78773:intron 20.0%
!! TCTTGTAAAAAAGTAATCAT+TGG + chr5.1:40800497-40800516 MS.gene78773:intron 20.0%
!! TTCAATCTGAAAATTGTAAA+TGG - chr5.1:40799814-40799833 None:intergenic 20.0%
!! TTTATACTTTACTCATATAG+AGG - chr5.1:40800751-40800770 None:intergenic 20.0%
!! TTTGTTAAAATTTCAACTCT+TGG - chr5.1:40800783-40800802 None:intergenic 20.0%
!!! ATACAAAATATTTTTGGGTT+GGG + chr5.1:40799672-40799691 MS.gene78773:CDS 20.0%
!!! ATTTTCAGATTGAAATTGAA+AGG + chr5.1:40799820-40799839 MS.gene78773:intron 20.0%
!!! GAAATGTGTTTATTTTCTAT+CGG + chr5.1:40799601-40799620 MS.gene78773:CDS 20.0%
!!! TTTTCAGATTGAAATTGAAA+GGG + chr5.1:40799821-40799840 MS.gene78773:intron 20.0%
!!! TTTTTTTAACTTCCACTAAA+TGG - chr5.1:40800429-40800448 None:intergenic 20.0%
! AAAATAGGAAATTGAACTAG+TGG - chr5.1:40800110-40800129 None:intergenic 25.0%
! AACTGACAAATAACAACAAT+TGG - chr5.1:40799754-40799773 None:intergenic 25.0%
! ACTGACAAATAACAACAATT+GGG - chr5.1:40799753-40799772 None:intergenic 25.0%
! CATACTTGCTTTAATATTCT+GGG + chr5.1:40799879-40799898 MS.gene78773:CDS 25.0%
! CTAGGTGAATTATATAATGT+CGG + chr5.1:40800397-40800416 MS.gene78773:intron 25.0%
! CTTGAAATTGGAAAATCATT+GGG + chr5.1:40800973-40800992 MS.gene78773:CDS 25.0%
! CTTTAGATTTAGGAAATTGT+CGG + chr5.1:40800007-40800026 MS.gene78773:intron 25.0%
! TGCTTGTTCTTAACATTTAT+TGG + chr5.1:40801044-40801063 MS.gene78773:CDS 25.0%
! TGTTCTTAACATTTATTGGT+GGG + chr5.1:40801048-40801067 MS.gene78773:CDS 25.0%
! TTGTTCTTAACATTTATTGG+TGG + chr5.1:40801047-40801066 MS.gene78773:CDS 25.0%
!! TATTTTGTATCAGTAAACCA+AGG - chr5.1:40799662-40799681 None:intergenic 25.0%
!! TCTTACTTAATCTTTTGATC+TGG - chr5.1:40799716-40799735 None:intergenic 25.0%
!! TTGTGTTATCCTACATTTTT+AGG + chr5.1:40799962-40799981 MS.gene78773:CDS 25.0%
!!! CTTAATTTTTCAATACACTC+AGG + chr5.1:40800131-40800150 MS.gene78773:intron 25.0%
!!! GATACAAAATATTTTTGGGT+TGG + chr5.1:40799671-40799690 MS.gene78773:CDS 25.0%
AAATGTAGGATAACACAATG+AGG - chr5.1:40799960-40799979 None:intergenic 30.0%
AATCATTGGTGAATGATGAT+AGG + chr5.1:40800511-40800530 MS.gene78773:intron 30.0%
CCAGAATATTAAAGCAAGTA+TGG - chr5.1:40799881-40799900 None:intergenic 30.0%
CCATACTTGCTTTAATATTC+TGG + chr5.1:40799878-40799897 MS.gene78773:CDS 30.0%
CCTTGAAATTGGAAAATCAT+TGG + chr5.1:40800972-40800991 MS.gene78773:CDS 30.0%
CGACAATTTCCTAAATCTAA+AGG - chr5.1:40800009-40800028 None:intergenic 30.0%
CTAAACTCTTAACAAAACAG+AGG + chr5.1:40800554-40800573 MS.gene78773:intron 30.0%
GTCTAAATGCCTTTAGATTT+AGG + chr5.1:40799997-40800016 MS.gene78773:intron 30.0%
! ATGATTTTCCAATTTCAAGG+TGG - chr5.1:40800972-40800991 None:intergenic 30.0%
! CCAATGATTTTCCAATTTCA+AGG - chr5.1:40800975-40800994 None:intergenic 30.0%
!! CTCTGTTTTGTTAAGAGTTT+AGG - chr5.1:40800556-40800575 None:intergenic 30.0%
!!! ATATGTTAGCTTTTCAACAG+TGG + chr5.1:40800703-40800722 MS.gene78773:intron 30.0%
!!! GGCTGTGCAATTTAATTTTT+TGG + chr5.1:40800575-40800594 MS.gene78773:intron 30.0%
!!! TTGGTTTTAAGAGCACATTT+TGG + chr5.1:40800044-40800063 MS.gene78773:intron 30.0%
ACAAATTTAAGGAATCAGCG+TGG + chr5.1:40799579-40799598 MS.gene78773:CDS 35.0%
ACCTAGATTAGCAACTACAA+TGG - chr5.1:40800383-40800402 None:intergenic 35.0%
CAGTTGTTTAATCCATCATG+AGG - chr5.1:40800649-40800668 None:intergenic 35.0%
GAAATCGCACCTAAAAATGT+AGG - chr5.1:40799974-40799993 None:intergenic 35.0%
GGTGAATTATATAATGTCGG+CGG + chr5.1:40800400-40800419 MS.gene78773:intron 35.0%
TACAAAAAGTGACCTCATGA+TGG + chr5.1:40800634-40800653 MS.gene78773:intron 35.0%
TACTTCGAAGAACCCAAAAT+GGG - chr5.1:40800315-40800334 None:intergenic 35.0%
TTGAAAGGGCTGTATATGTA+TGG + chr5.1:40799835-40799854 MS.gene78773:CDS 35.0%
TTGGAAAATCATTGGGTGAA+TGG + chr5.1:40800980-40800999 MS.gene78773:CDS 35.0%
TTGGTGAATGATGATAGGAT+AGG + chr5.1:40800516-40800535 MS.gene78773:intron 35.0%
!! AGACGGTTCATTTTTGGAAA+GGG + chr5.1:40799509-40799528 MS.gene78773:CDS 35.0%
!! GCAACTGAGGGAATTTTAAT+AGG - chr5.1:40800361-40800380 None:intergenic 35.0%
!! TGTCGTTTACTACCCATTTT+GGG + chr5.1:40800300-40800319 MS.gene78773:CDS 35.0%
AAACAAGACGCTCTGACTTT+GGG + chr5.1:40799902-40799921 MS.gene78773:CDS 40.0%
CTTTCCAAAAATGAACCGTC+TGG - chr5.1:40799510-40799529 None:intergenic 40.0%
GCCATTGTAGTTGCTAATCT+AGG + chr5.1:40800379-40800398 MS.gene78773:intron 40.0%
GGGAAAATGTCCAAATACAG+CGG + chr5.1:40800217-40800236 MS.gene78773:CDS 40.0%
GTACTTCGAAGAACCCAAAA+TGG - chr5.1:40800316-40800335 None:intergenic 40.0%
GTAGTAAACGACACGTAGTA+TGG - chr5.1:40800293-40800312 None:intergenic 40.0%
TCTTTGTTGCAGCTATGAAC+TGG + chr5.1:40800943-40800962 MS.gene78773:intron 40.0%
TGATGCTACTGATGTGTTTC+TGG + chr5.1:40800189-40800208 MS.gene78773:CDS 40.0%
TTGGTCAAATTCGAGCAAGA+GGG + chr5.1:40801092-40801111 MS.gene78773:CDS 40.0%
! ACACTCAGGTTTGTTCGAAT+TGG + chr5.1:40800145-40800164 MS.gene78773:intron 40.0%
! TTTGGGTTCTTCGAAGTACA+AGG + chr5.1:40800317-40800336 MS.gene78773:CDS 40.0%
!! ACTGATGTGTTTCTGGAGAT+AGG + chr5.1:40800196-40800215 MS.gene78773:CDS 40.0%
!! CAGACGGTTCATTTTTGGAA+AGG + chr5.1:40799508-40799527 MS.gene78773:CDS 40.0%
!! CTGATGTGTTTCTGGAGATA+GGG + chr5.1:40800197-40800216 MS.gene78773:CDS 40.0%
!! TTTTTCAGCACCGCTGTATT+TGG - chr5.1:40800230-40800249 None:intergenic 40.0%
!!! AAATATTTTTGGGTTGGGCC+AGG + chr5.1:40799677-40799696 MS.gene78773:CDS 40.0%
!!! AATATTTTTGGGTTGGGCCA+GGG + chr5.1:40799678-40799697 MS.gene78773:CDS 40.0%
!!! GTGTCGTTTACTACCCATTT+TGG + chr5.1:40800299-40800318 MS.gene78773:CDS 40.0%
ACTGGTTGCCACCTTGAAAT+TGG + chr5.1:40800961-40800980 MS.gene78773:CDS 45.0%
AGCAACTACAATGGCAACTG+AGG - chr5.1:40800374-40800393 None:intergenic 45.0%
CTGATTCTTCGGATGCTTGT+TGG + chr5.1:40801073-40801092 MS.gene78773:CDS 45.0%
GAAACAAGACGCTCTGACTT+TGG + chr5.1:40799901-40799920 MS.gene78773:CDS 45.0%
GCAACTACAATGGCAACTGA+GGG - chr5.1:40800373-40800392 None:intergenic 45.0%
GTTGGTCAAATTCGAGCAAG+AGG + chr5.1:40801091-40801110 MS.gene78773:CDS 45.0%
! GGAAATTGTCGGTGTCTCTA+CGG + chr5.1:40800018-40800037 MS.gene78773:intron 45.0%
! TTGGCCAGACGGTTCATTTT+TGG + chr5.1:40799503-40799522 MS.gene78773:CDS 45.0%
!! ATTGGTGGGTGCTGATTCTT+CGG + chr5.1:40801062-40801081 MS.gene78773:CDS 45.0%
CTGTGACATATGACGAGCCT+TGG + chr5.1:40799642-40799661 MS.gene78773:CDS 50.0%
GGGCTGTATATGTATGGTGC+TGG + chr5.1:40799841-40799860 MS.gene78773:CDS 50.0%
TGTCGGCGGTAACCATTTAG+TGG + chr5.1:40800414-40800433 MS.gene78773:intron 50.0%
! GTCGGTGTCTCTACGGAAAT+TGG + chr5.1:40800025-40800044 MS.gene78773:intron 50.0%
TCTGGCCATCTCTGATTCCC+TGG - chr5.1:40799698-40799717 None:intergenic 55.0%
TTGGGCCAGGGAATCAGAGA+TGG + chr5.1:40799690-40799709 MS.gene78773:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 40799500 40801139 40799500 ID=MS.gene78773
chr5.1 mRNA 40799500 40801139 40799500 ID=MS.gene78773.t1;Parent=MS.gene78773
chr5.1 exon 40799500 40799729 40799500 ID=MS.gene78773.t1.exon1;Parent=MS.gene78773.t1
chr5.1 CDS 40799500 40799729 40799500 ID=cds.MS.gene78773.t1;Parent=MS.gene78773.t1
chr5.1 exon 40799828 40799983 40799828 ID=MS.gene78773.t1.exon2;Parent=MS.gene78773.t1
chr5.1 CDS 40799828 40799983 40799828 ID=cds.MS.gene78773.t1;Parent=MS.gene78773.t1
chr5.1 exon 40800153 40800338 40800153 ID=MS.gene78773.t1.exon3;Parent=MS.gene78773.t1
chr5.1 CDS 40800153 40800338 40800153 ID=cds.MS.gene78773.t1;Parent=MS.gene78773.t1
chr5.1 exon 40800955 40801139 40800955 ID=MS.gene78773.t1.exon4;Parent=MS.gene78773.t1
chr5.1 CDS 40800955 40801139 40800955 ID=cds.MS.gene78773.t1;Parent=MS.gene78773.t1
Gene Sequence

>MS.gene78773

AAATTGGCCAGACGGTTCATTTTTGGAAAGGGACATGAAGTGCTTGATTTTCATACAGATGATAGTAGAAAGAAGATTAACAAATTTAAGGAATCAGCGTGGAAATGTGTTTATTTTCTATCGGCAGAAATTTTTGCGTTGTCTGTGACATATGACGAGCCTTGGTTTACTGATACAAAATATTTTTGGGTTGGGCCAGGGAATCAGAGATGGCCAGATCAAAAGATTAAGTAAGATTTTTATTTACATGCCCAATTGTTGTTATTTGTCAGTTGTCATTTCAAGTTAGTAGTTAACTATCGATTGAGTAACCATTTACAATTTTCAGATTGAAATTGAAAGGGCTGTATATGTATGGTGCTGGATTTTATTCATACTCCATACTTGCTTTAATATTCTGGGAAACAAGACGCTCTGACTTTGGGATTTCTATGACACATCATGTTGCTTCTCTCAGCCTCATTGTGTTATCCTACATTTTTAGGTGCGATTTCATTGTCTAAATGCCTTTAGATTTAGGAAATTGTCGGTGTCTCTACGGAAATTGGTTTTAAGAGCACATTTTGGACTTAATGTCTTCACTAATTCTATTTTATTTAAATTTACTCCACTAGTTCAATTTCCTATTTTACTTAATTTTTCAATACACTCAGGTTTGTTCGAATTGGATCAGTTGTTTTAGCTCTTCATGATGCTACTGATGTGTTTCTGGAGATAGGGAAAATGTCCAAATACAGCGGTGCTGAAAAAATCGCTAGCTTTGCATTTGTTCTGTTTGTTTTATCTTTCACCATACTACGTGTCGTTTACTACCCATTTTGGGTTCTTCGAAGTACAAGGTTTGTTTTCTTTCTTTCACCTATTAAAATTCCCTCAGTTGCCATTGTAGTTGCTAATCTAGGTGAATTATATAATGTCGGCGGTAACCATTTAGTGGAAGTTAAAAAAATCCCAAATGTAAATTTAAAGGATATGATCAGTTTGCTGCTCTTCTTAATCTTGTAAAAAAGTAATCATTGGTGAATGATGATAGGATAGGTACACTTTTTTTCTCCTAAACTCTTAACAAAACAGAGGCTGTGCAATTTAATTTTTTGGATTTTACTTCTAAAGTGAGCAATTCTCTTCAGACGATACAAAAAGTGACCTCATGATGGATTAAACAACTGTTTATATTGCGTTTATATGCATAACAAATCATATATATGTTAGCTTTTCAACAGTGGATTTTCTCATCAATAGCTGACACCTCTATATGAGTAAAGTATAAAGTGGAAAAACCAAGAGTTGAAATTTTAACAAACTCGTAATTTGTCATGATTTATCTAGAATATTCATCTTTGACTTGTTAATTAATAATTGATAATACTCATCACATAGTCTAAAATAAATTACTCAGTTTATACGGACATATACTTTTATTTCTCTATTTAATTGTTTTATTCTTTGTTGCAGCTATGAACTGGTTGCCACCTTGAAATTGGAAAATCATTGGGTGAATGGTTCTATACATTATTATGTATTCAATAGTCTTCTATTCTGCTTGCTTGTTCTTAACATTTATTGGTGGGTGCTGATTCTTCGGATGCTTGTTGGTCAAATTCGAGCAAGAGGGAAAGTTAGTGAAGATATACGATCTG

Protein sequence

>MS.gene78773.t1

KLARRFIFGKGHEVLDFHTDDSRKKINKFKESAWKCVYFLSAEIFALSVTYDEPWFTDTKYFWVGPGNQRWPDQKIKLKLKGLYMYGAGFYSYSILALIFWETRRSDFGISMTHHVASLSLIVLSYIFRFVRIGSVVLALHDATDVFLEIGKMSKYSGAEKIASFAFVLFVLSFTILRVVYYPFWVLRSTSYELVATLKLENHWVNGSIHYYVFNSLLFCLLVLNIYWWVLILRMLVGQIRARGKVSEDIRS