Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80782.t1 | XP_013455005.1 | 97.9 | 704 | 15 | 0 | 20 | 723 | 84 | 787 | 0.00E+00 | 1348.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80782.t1 | Q6NQJ6 | 71.8 | 696 | 195 | 1 | 27 | 721 | 108 | 803 | 1.4e-287 | 989.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80782.t1 | A0A072UIX7 | 97.9 | 704 | 15 | 0 | 20 | 723 | 84 | 787 | 0.0e+00 | 1348.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene80782 | 0.824588 | 7.44E-54 | -1.69E-46 |
| MS.gene049222 | MS.gene80782 | 0.835214 | 1.91E-56 | -1.69E-46 |
| MS.gene049427 | MS.gene80782 | 0.832721 | 8.04E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene80782 | 0.801132 | 1.04E-48 | -1.69E-46 |
| MS.gene049633 | MS.gene80782 | 0.809306 | 2.02E-50 | -1.69E-46 |
| MS.gene050538 | MS.gene80782 | 0.812014 | 5.25E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene80782 | 0.804518 | 2.08E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene80782 | 0.830294 | 3.18E-55 | -1.69E-46 |
| MS.gene050747 | MS.gene80782 | 0.878569 | 2.52E-69 | -1.69E-46 |
| MS.gene050772 | MS.gene80782 | 0.812461 | 4.19E-51 | -1.69E-46 |
| MS.gene050812 | MS.gene80782 | 0.843322 | 1.50E-58 | -1.69E-46 |
| MS.gene05081 | MS.gene80782 | 0.802902 | 4.50E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene80782 | 0.881781 | 1.80E-70 | -1.69E-46 |
| MS.gene051387 | MS.gene80782 | 0.830111 | 3.53E-55 | -1.69E-46 |
| MS.gene051525 | MS.gene80782 | 0.806142 | 9.50E-50 | -1.69E-46 |
| MS.gene051666 | MS.gene80782 | 0.828041 | 1.12E-54 | -1.69E-46 |
| MS.gene051694 | MS.gene80782 | 0.818783 | 1.64E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene80782 | 0.817768 | 2.78E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene80782 | 0.802663 | 5.04E-49 | -1.69E-46 |
| MS.gene052346 | MS.gene80782 | 0.802182 | 6.33E-49 | -1.69E-46 |
| MS.gene052481 | MS.gene80782 | 0.802414 | 5.67E-49 | -1.69E-46 |
| MS.gene052501 | MS.gene80782 | 0.833883 | 4.13E-56 | -1.69E-46 |
| MS.gene052503 | MS.gene80782 | 0.816345 | 5.80E-52 | -1.69E-46 |
| MS.gene052505 | MS.gene80782 | 0.830436 | 2.94E-55 | -1.69E-46 |
| MS.gene052615 | MS.gene80782 | 0.810373 | 1.19E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene80782 | 0.813818 | 2.11E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene80782 | 0.816272 | 6.02E-52 | -1.69E-46 |
| MS.gene052982 | MS.gene80782 | 0.816831 | 4.52E-52 | -1.69E-46 |
| MS.gene053107 | MS.gene80782 | 0.853174 | 2.85E-61 | -1.69E-46 |
| MS.gene053203 | MS.gene80782 | 0.840199 | 1.00E-57 | -1.69E-46 |
| MS.gene053213 | MS.gene80782 | 0.818375 | 2.03E-52 | -1.69E-46 |
| MS.gene053286 | MS.gene80782 | 0.811049 | 8.50E-51 | -1.69E-46 |
| MS.gene053436 | MS.gene80782 | 0.817352 | 3.45E-52 | -1.69E-46 |
| MS.gene053574 | MS.gene80782 | 0.827083 | 1.90E-54 | -1.69E-46 |
| MS.gene053579 | MS.gene80782 | 0.818381 | 2.02E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene80782 | 0.822598 | 2.17E-53 | -1.69E-46 |
| MS.gene054408 | MS.gene80782 | 0.854851 | 9.34E-62 | -1.69E-46 |
| MS.gene054884 | MS.gene80782 | -0.824014 | 1.02E-53 | -1.69E-46 |
| MS.gene054927 | MS.gene80782 | 0.809701 | 1.66E-50 | -1.69E-46 |
| MS.gene055224 | MS.gene80782 | 0.810929 | 9.03E-51 | -1.69E-46 |
| MS.gene055539 | MS.gene80782 | 0.812214 | 4.75E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene80782 | 0.83224 | 1.06E-55 | -1.69E-46 |
| MS.gene05589 | MS.gene80782 | 0.800554 | 1.36E-48 | -1.69E-46 |
| MS.gene056166 | MS.gene80782 | 0.814672 | 1.37E-51 | -1.69E-46 |
| MS.gene056409 | MS.gene80782 | 0.815113 | 1.09E-51 | -1.69E-46 |
| MS.gene056549 | MS.gene80782 | 0.80743 | 5.07E-50 | -1.69E-46 |
| MS.gene056610 | MS.gene80782 | 0.82256 | 2.22E-53 | -1.69E-46 |
| MS.gene05666 | MS.gene80782 | 0.811518 | 6.73E-51 | -1.69E-46 |
| MS.gene056692 | MS.gene80782 | 0.800955 | 1.13E-48 | -1.69E-46 |
| MS.gene056877 | MS.gene80782 | 0.836221 | 1.06E-56 | -1.69E-46 |
| MS.gene057011 | MS.gene80782 | 0.822711 | 2.05E-53 | -1.69E-46 |
| MS.gene057146 | MS.gene80782 | 0.801466 | 8.89E-49 | -1.69E-46 |
| MS.gene057332 | MS.gene80782 | 0.808404 | 3.15E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene80782 | 0.808973 | 2.38E-50 | -1.69E-46 |
| MS.gene057658 | MS.gene80782 | 0.835451 | 1.67E-56 | -1.69E-46 |
| MS.gene057831 | MS.gene80782 | 0.823578 | 1.28E-53 | -1.69E-46 |
| MS.gene058338 | MS.gene80782 | 0.80476 | 1.85E-49 | -1.69E-46 |
| MS.gene058376 | MS.gene80782 | 0.807135 | 5.86E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene80782 | 0.835714 | 1.43E-56 | -1.69E-46 |
| MS.gene058444 | MS.gene80782 | 0.815898 | 7.30E-52 | -1.69E-46 |
| MS.gene058544 | MS.gene80782 | 0.818034 | 2.42E-52 | -1.69E-46 |
| MS.gene058700 | MS.gene80782 | 0.828288 | 9.77E-55 | -1.69E-46 |
| MS.gene058838 | MS.gene80782 | 0.835451 | 1.67E-56 | -1.69E-46 |
| MS.gene05883 | MS.gene80782 | 0.843888 | 1.06E-58 | -1.69E-46 |
| MS.gene059023 | MS.gene80782 | 0.841627 | 4.24E-58 | -1.69E-46 |
| MS.gene05934 | MS.gene80782 | 0.844442 | 7.54E-59 | -1.69E-46 |
| MS.gene059536 | MS.gene80782 | 0.817545 | 3.12E-52 | -1.69E-46 |
| MS.gene059718 | MS.gene80782 | 0.80457 | 2.03E-49 | -1.69E-46 |
| MS.gene059756 | MS.gene80782 | 0.807909 | 4.01E-50 | -1.69E-46 |
| MS.gene059801 | MS.gene80782 | 0.817432 | 3.31E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene80782 | 0.814448 | 1.53E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene80782 | 0.822866 | 1.88E-53 | -1.69E-46 |
| MS.gene06092 | MS.gene80782 | 0.82698 | 2.01E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene80782 | 0.801468 | 8.88E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene80782 | 0.823823 | 1.13E-53 | -1.69E-46 |
| MS.gene061231 | MS.gene80782 | 0.807196 | 5.69E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80782.t1 | MTR_4g021750 | 97.869 | 704 | 15 | 0 | 20 | 723 | 84 | 787 | 0.0 | 1418 |
| MS.gene80782.t1 | MTR_4g021750 | 98.046 | 614 | 12 | 0 | 110 | 723 | 1 | 614 | 0.0 | 1236 |
| MS.gene80782.t1 | MTR_4g021750 | 97.978 | 544 | 11 | 0 | 180 | 723 | 12 | 555 | 0.0 | 1094 |
| MS.gene80782.t1 | MTR_8g073170 | 25.127 | 394 | 249 | 10 | 318 | 669 | 470 | 859 | 9.95e-21 | 98.2 |
| MS.gene80782.t1 | MTR_5g059080 | 25.138 | 362 | 203 | 10 | 317 | 615 | 507 | 863 | 1.28e-15 | 81.6 |
| MS.gene80782.t1 | MTR_5g058810 | 24.716 | 352 | 197 | 10 | 327 | 615 | 482 | 828 | 8.49e-13 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80782.t1 | AT5G02250 | 71.839 | 696 | 195 | 1 | 27 | 721 | 108 | 803 | 0.0 | 1001 |
| MS.gene80782.t1 | AT2G17510 | 25.500 | 400 | 240 | 13 | 318 | 669 | 469 | 858 | 2.05e-24 | 109 |
| MS.gene80782.t1 | AT2G17510 | 24.515 | 412 | 241 | 14 | 318 | 669 | 536 | 937 | 5.82e-21 | 99.0 |
| MS.gene80782.t1 | AT1G77680 | 32.576 | 132 | 89 | 0 | 318 | 449 | 476 | 607 | 1.47e-12 | 72.0 |
Find 153 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGATTGCGTTGCGTTTA+TGG | 0.132721 | 4.4:+79924854 | MS.gene80782:CDS |
| CTCTCCCATCCGTAGATATT+TGG | 0.150105 | 4.4:+79931433 | MS.gene80782:CDS |
| GCCAGCACGCCACGGGGTTT+TGG | 0.196686 | 4.4:+79931385 | MS.gene80782:CDS |
| GCACGGTATCTTCTCTCTTT+TGG | 0.229092 | 4.4:-79932542 | None:intergenic |
| CTGCAAATCTTCGGTTAAAT+TGG | 0.237762 | 4.4:+79930246 | MS.gene80782:CDS |
| CAGAACTTCATTAAACTATT+AGG | 0.256770 | 4.4:+79926438 | MS.gene80782:CDS |
| CTCATCCTTCCACGAAGATT+TGG | 0.265456 | 4.4:-79926496 | None:intergenic |
| GGACCTTCTTGCTCATTATC+AGG | 0.293979 | 4.4:+79931454 | MS.gene80782:CDS |
| TTTCACATGTTTAGATTAAC+AGG | 0.295584 | 4.4:+79928748 | MS.gene80782:intron |
| GGAAGGATGAGGAGAAAATT+TGG | 0.297565 | 4.4:+79926508 | MS.gene80782:CDS |
| CCAACAGTAGTCTCCGTTAC+TGG | 0.304352 | 4.4:+79932495 | MS.gene80782:CDS |
| GTGCAGCTGAAATTGATTTC+AGG | 0.320002 | 4.4:+79931360 | MS.gene80782:CDS |
| GTGAAGCGTTCCTTGCAAAA+AGG | 0.320722 | 4.4:+79924157 | MS.gene80782:CDS |
| AAGCTCCTAACAGGCCCTTC+TGG | 0.330580 | 4.4:-79931317 | None:intergenic |
| TCAGAGACTAATCGCATTGC+AGG | 0.338662 | 4.4:-79931179 | None:intergenic |
| GATATCTGCTTGGTCAAAAT+TGG | 0.344009 | 4.4:-79924466 | None:intergenic |
| CAGATAAAGGCATTTCTTAG+AGG | 0.347763 | 4.4:+79932389 | MS.gene80782:intron |
| CTTGCAGATACTAAAGGAAA+TGG | 0.367872 | 4.4:+79927083 | MS.gene80782:intron |
| CATCACTACTTGAATTTGCT+TGG | 0.374236 | 4.4:+79924615 | MS.gene80782:CDS |
| GACCTGATGGCAAGAAGAAC+TGG | 0.375215 | 4.4:+79924236 | MS.gene80782:CDS |
| CATCAATGGCATAGACCTTC+AGG | 0.382711 | 4.4:-79928785 | None:intergenic |
| TCTACATAAAGGTTTATTGA+TGG | 0.385755 | 4.4:-79931143 | None:intergenic |
| TAAGTTCAACCTCCTCTTCA+AGG | 0.387057 | 4.4:-79930208 | None:intergenic |
| AGGGGATGAAGTAGTGGTTA+AGG | 0.387235 | 4.4:+79933733 | MS.gene80782:CDS |
| AAACCTTTATGTAGAAAATC+AGG | 0.389129 | 4.4:+79931151 | MS.gene80782:CDS |
| GAACTGGATGGTTTCAGATC+AGG | 0.391570 | 4.4:+79924252 | MS.gene80782:CDS |
| TGTCACTTGTTGTGGCTTGA+TGG | 0.394461 | 4.4:-79924424 | None:intergenic |
| TGCAGTTCTGTTGTTGGTTG+AGG | 0.395036 | 4.4:+79932595 | MS.gene80782:CDS |
| TTACCGCTTTCCCATTGCTT+TGG | 0.396461 | 4.4:+79924061 | MS.gene80782:CDS |
| ATTGATGGCTCTGGATCCTC+TGG | 0.396536 | 4.4:-79931128 | None:intergenic |
| ATCCAGTTCTTCTTGCCATC+AGG | 0.396705 | 4.4:-79924238 | None:intergenic |
| ATCTTCCAGAAGGGCCTGTT+AGG | 0.398842 | 4.4:+79931312 | MS.gene80782:CDS |
| AAGATGGTCGGATTAACATT+TGG | 0.401203 | 4.4:+79928957 | MS.gene80782:CDS |
| CCCAAAACCCCGTGGCGTGC+TGG | 0.403412 | 4.4:-79931386 | None:intergenic |
| TTGGATTTCAAGCTACTGCT+TGG | 0.404806 | 4.4:+79933690 | MS.gene80782:CDS |
| ACTAGGGCAAAGCTCCTAAC+AGG | 0.406665 | 4.4:-79931326 | None:intergenic |
| TCGCATTGCAGTTCTGTTGT+TGG | 0.414465 | 4.4:+79932589 | MS.gene80782:CDS |
| TGGATTTCAAGCTACTGCTT+GGG | 0.414963 | 4.4:+79933691 | MS.gene80782:CDS |
| TACCTATCCACAATACTTCC+AGG | 0.417222 | 4.4:-79929013 | None:intergenic |
| TAAAGGTTTATTGATGGCTC+TGG | 0.421693 | 4.4:-79931137 | None:intergenic |
| GTTAAATTGGCGCCGCCAGC+AGG | 0.432558 | 4.4:+79930259 | MS.gene80782:CDS |
| GGCGAAGCTATAGCTACATT+TGG | 0.436547 | 4.4:+79931218 | MS.gene80782:CDS |
| CATAGACCTTCAGGTTAGTA+AGG | 0.437715 | 4.4:-79928776 | None:intergenic |
| AAATCAAGATTGACATGTAT+AGG | 0.439477 | 4.4:-79927162 | None:intergenic |
| GTCTCAGTTGGATCACAGAT+AGG | 0.448663 | 4.4:+79933713 | MS.gene80782:CDS |
| TCAGGAAGCCAGCACGCCAC+GGG | 0.449427 | 4.4:+79931378 | MS.gene80782:CDS |
| TAACAGGCCCTTCTGGAAGA+TGG | 0.456437 | 4.4:-79931310 | None:intergenic |
| TGAGGAGCTTATTCGTAGAA+AGG | 0.460349 | 4.4:+79926386 | MS.gene80782:CDS |
| ATAACATTCCTTTACCCTAC+AGG | 0.466109 | 4.4:+79931249 | MS.gene80782:CDS |
| CAAGTGACATATATTGTTCC+CGG | 0.469433 | 4.4:+79924438 | MS.gene80782:CDS |
| GAAAGATTTATATCTGATTG+AGG | 0.479892 | 4.4:-79931278 | None:intergenic |
| TGTCTGAGGCTGCAAATCTT+CGG | 0.486597 | 4.4:+79930237 | MS.gene80782:CDS |
| GAGCCTCCACCCTTTACATC+TGG | 0.487578 | 4.4:+79932413 | MS.gene80782:CDS |
| ATGCTTCTTGCAGATACTAA+AGG | 0.494187 | 4.4:+79927077 | MS.gene80782:intron |
| CAAGCAAATTCAAGTAGTGA+TGG | 0.494516 | 4.4:-79924614 | None:intergenic |
| TTCGAGATTACCAGATGTAA+AGG | 0.496296 | 4.4:-79932423 | None:intergenic |
| ATAAACCTTCCAAATCTTCG+TGG | 0.496392 | 4.4:+79926487 | MS.gene80782:CDS |
| ACAATATATGTCACTTGTTG+TGG | 0.497382 | 4.4:-79924432 | None:intergenic |
| TTGGTCAAAATTGGATATGC+CGG | 0.499648 | 4.4:-79924457 | None:intergenic |
| CAAATCTTCGTGGAAGGATG+AGG | 0.500601 | 4.4:+79926497 | MS.gene80782:CDS |
| CTATAGCTACATTTGGATCT+CGG | 0.500967 | 4.4:+79931225 | MS.gene80782:CDS |
| GCTACTGCTTGGGTCTCAGT+TGG | 0.501138 | 4.4:+79933701 | MS.gene80782:CDS |
| TTGCAGATACTAAAGGAAAT+GGG | 0.505523 | 4.4:+79927084 | MS.gene80782:intron |
| AATACATCTACCTGCTCACT+TGG | 0.505938 | 4.4:-79924884 | None:intergenic |
| TTTACTATACTCGAATCTAA+AGG | 0.510666 | 4.4:+79924836 | MS.gene80782:CDS |
| GAGAATTCCCACTGATCACT+TGG | 0.511382 | 4.4:+79927194 | MS.gene80782:CDS |
| TAGATAGTAACCAAAGCAAT+GGG | 0.511508 | 4.4:-79924071 | None:intergenic |
| GATACTAAAGGAAATGGGTC+TGG | 0.515799 | 4.4:+79927089 | MS.gene80782:intron |
| TCTCAGTTGGATCACAGATA+GGG | 0.517800 | 4.4:+79933714 | MS.gene80782:CDS |
| CGATATTATTTATGTGAAGG+AGG | 0.518913 | 4.4:+79933778 | MS.gene80782:CDS |
| AACAGGCCCTTCTGGAAGAT+GGG | 0.523166 | 4.4:-79931309 | None:intergenic |
| ATCAGCTTCATCAACATCAA+TGG | 0.525986 | 4.4:-79928799 | None:intergenic |
| GATGAACAAAGGAAAACAGC+TGG | 0.530150 | 4.4:+79926579 | MS.gene80782:CDS |
| AACATAGAATATGTAGCAGT+GGG | 0.531882 | 4.4:-79929147 | None:intergenic |
| GGTTGTCCCCTGTAGGGTAA+AGG | 0.533718 | 4.4:-79931257 | None:intergenic |
| CCAGTAACGGAGACTACTGT+TGG | 0.534199 | 4.4:-79932495 | None:intergenic |
| ATCTCTTCCAAGTGATCAGT+GGG | 0.536634 | 4.4:-79927201 | None:intergenic |
| TCGAGATTACCAGATGTAAA+GGG | 0.539275 | 4.4:-79932422 | None:intergenic |
| CAATTTGAACCTTGAAGAGG+AGG | 0.541680 | 4.4:+79930199 | MS.gene80782:CDS |
| TTCAGGAAGCCAGCACGCCA+CGG | 0.541815 | 4.4:+79931377 | MS.gene80782:CDS |
| CTGCACGCATTATCTTGACT+AGG | 0.542237 | 4.4:-79931343 | None:intergenic |
| TGGTCAAAATTGGATATGCC+GGG | 0.542295 | 4.4:-79924456 | None:intergenic |
| TGATTGAGGTTGTCCCCTGT+AGG | 0.545539 | 4.4:-79931264 | None:intergenic |
| TTGGAAGGTTTATCATGTGA+AGG | 0.550274 | 4.4:-79926477 | None:intergenic |
| GTTTCCAGAAAATCTTGCCA+TGG | 0.550462 | 4.4:+79929167 | MS.gene80782:CDS |
| CAAGAAGGTCCAAATATCTA+CGG | 0.550736 | 4.4:-79931442 | None:intergenic |
| TGGACCTCGACCAAGTGAGC+AGG | 0.555090 | 4.4:+79924874 | MS.gene80782:CDS |
| AAGGTCCAAATATCTACGGA+TGG | 0.556668 | 4.4:-79931438 | None:intergenic |
| TTAGATAGTAACCAAAGCAA+TGG | 0.557269 | 4.4:-79924072 | None:intergenic |
| TAACATTCCTTTACCCTACA+GGG | 0.558376 | 4.4:+79931250 | MS.gene80782:CDS |
| GATTGAGGTTGTCCCCTGTA+GGG | 0.558678 | 4.4:-79931263 | None:intergenic |
| TATTTGCTGGTTTGACAGCA+CGG | 0.559960 | 4.4:+79924099 | MS.gene80782:CDS |
| TGACGATATTATTTATGTGA+AGG | 0.560650 | 4.4:+79933775 | MS.gene80782:CDS |
| TATGTGAAGGAGGTTGTAAG+AGG | 0.562265 | 4.4:+79933788 | MS.gene80782:CDS |
| TGTTCAACCTGGAAGTATTG+TGG | 0.567891 | 4.4:+79929006 | MS.gene80782:CDS |
| TTTCCAGAAAATCTTGCCAT+GGG | 0.567903 | 4.4:+79929168 | MS.gene80782:CDS |
| AATCTCGAAGGTCTAGCAGC+AGG | 0.568622 | 4.4:+79932437 | MS.gene80782:CDS |
| TTACCAGATGTAAAGGGTGG+AGG | 0.571399 | 4.4:-79932416 | None:intergenic |
| CAAATATCTACGGATGGGAG+AGG | 0.574656 | 4.4:-79931432 | None:intergenic |
| TCCTTCCACGAAGATTTGGA+AGG | 0.578547 | 4.4:-79926492 | None:intergenic |
| CAAAATGTGCGATATCTGCT+TGG | 0.581110 | 4.4:-79924476 | None:intergenic |
| TTGTGCTGCATGACGATGGC+AGG | 0.582075 | 4.4:+79929241 | MS.gene80782:CDS |
| ACCTTCCAAATCTTCGTGGA+AGG | 0.584512 | 4.4:+79926491 | MS.gene80782:CDS |
| ACTCAATTTGAACCTTGAAG+AGG | 0.594338 | 4.4:+79930196 | MS.gene80782:CDS |
| TCATATGTTAACATGTACGT+TGG | 0.594529 | 4.4:-79930158 | None:intergenic |
| GTCTGTGACAGTTGAAGAGA+TGG | 0.594829 | 4.4:+79924661 | MS.gene80782:CDS |
| TGCACGCATTATCTTGACTA+GGG | 0.594838 | 4.4:-79931342 | None:intergenic |
| CACAGGGAGGCTATGAGAAG+AGG | 0.598459 | 4.4:+79929108 | MS.gene80782:intron |
| AAGCAAATTCAAGTAGTGAT+GGG | 0.598610 | 4.4:-79924613 | None:intergenic |
| AGGTCCAAATATCTACGGAT+GGG | 0.599467 | 4.4:-79931437 | None:intergenic |
| AAAATTTAAGGACTAAAGCA+CGG | 0.603113 | 4.4:-79932559 | None:intergenic |
| CTTGTATAAGTGATGAACAA+AGG | 0.604324 | 4.4:+79926568 | MS.gene80782:CDS |
| ACAGATAGGGGATGAAGTAG+TGG | 0.607271 | 4.4:+79933727 | MS.gene80782:CDS |
| CTCAGTTGGATCACAGATAG+GGG | 0.608551 | 4.4:+79933715 | MS.gene80782:CDS |
| GAAGAACCTTACTAACCTGA+AGG | 0.608563 | 4.4:+79928770 | MS.gene80782:CDS |
| AAACATAGAATATGTAGCAG+TGG | 0.610030 | 4.4:-79929148 | None:intergenic |
| TCTACCTGCTCACTTGGTCG+AGG | 0.611987 | 4.4:-79924878 | None:intergenic |
| GCAGTAGCTCAGAGACCTGA+TGG | 0.616317 | 4.4:+79924223 | MS.gene80782:CDS |
| TATTCGTAGAAAGGTTGCAA+AGG | 0.616448 | 4.4:+79926395 | MS.gene80782:CDS |
| TCTCAAACTCATAGCTCCCA+TGG | 0.618125 | 4.4:-79929184 | None:intergenic |
| CAACAAAGTTACAAGATGGT+CGG | 0.620904 | 4.4:+79928945 | MS.gene80782:CDS |
| TATGAGTTTGAGACAAGGGG+AGG | 0.621810 | 4.4:+79929194 | MS.gene80782:CDS |
| GATCTCTTCCAAGTGATCAG+TGG | 0.623686 | 4.4:-79927202 | None:intergenic |
| AGCTATGAGTTTGAGACAAG+GGG | 0.625008 | 4.4:+79929191 | MS.gene80782:CDS |
| ATGAACAAAGGAAAACAGCT+GGG | 0.625112 | 4.4:+79926580 | MS.gene80782:CDS |
| GAGGTAAATTGAACATAACC+AGG | 0.626585 | 4.4:-79931413 | None:intergenic |
| TTTACATCTGGTAATCTCGA+AGG | 0.634450 | 4.4:+79932425 | MS.gene80782:CDS |
| CAGAGACTAATCGCATTGCA+GGG | 0.637990 | 4.4:-79931178 | None:intergenic |
| ATGAGAAAGTTAGAGCAGTG+AGG | 0.639786 | 4.4:+79932465 | MS.gene80782:CDS |
| ATACCTGATAATGAGCAAGA+AGG | 0.641739 | 4.4:-79931457 | None:intergenic |
| TCAGAAAGCTCAGGACAACA+TGG | 0.644937 | 4.4:+79924500 | MS.gene80782:CDS |
| AGTATTCTAGTATCCAGTAA+CGG | 0.646549 | 4.4:-79932508 | None:intergenic |
| AGTGCAACAAAGTTACAAGA+TGG | 0.646571 | 4.4:+79928941 | MS.gene80782:CDS |
| AACCTGGAAGTATTGTGGAT+AGG | 0.646902 | 4.4:+79929011 | MS.gene80782:CDS |
| GAGCTATGAGTTTGAGACAA+GGG | 0.648036 | 4.4:+79929190 | MS.gene80782:CDS |
| TGATGTTGATGAAGCTGATG+AGG | 0.650498 | 4.4:+79928803 | MS.gene80782:CDS |
| TAACCAAAGCAATGGGAAAG+CGG | 0.650688 | 4.4:-79924064 | None:intergenic |
| GCAACTAGATATGTTCAACC+TGG | 0.651244 | 4.4:+79928995 | MS.gene80782:CDS |
| CAGGAAGCCAGCACGCCACG+GGG | 0.652514 | 4.4:+79931379 | MS.gene80782:CDS |
| AGATTACCAGATGTAAAGGG+TGG | 0.659485 | 4.4:-79932419 | None:intergenic |
| TGAGCAGAATAACTCTCGAG+AGG | 0.659723 | 4.4:-79924785 | None:intergenic |
| AACATTCCTTTACCCTACAG+GGG | 0.659934 | 4.4:+79931251 | MS.gene80782:CDS |
| AGATTCATCTGATCCAGATG+AGG | 0.661884 | 4.4:+79927251 | MS.gene80782:CDS |
| GACAGTTGAAGAGATGGCTG+AGG | 0.671335 | 4.4:+79924667 | MS.gene80782:CDS |
| TCAGTTGTGCTGCATGACGA+TGG | 0.673832 | 4.4:+79929237 | MS.gene80782:CDS |
| CATCAAAGTGTCTAATCCAG+AGG | 0.679471 | 4.4:+79931112 | MS.gene80782:CDS |
| TGATGGCAAGAAGAACTGGA+TGG | 0.679573 | 4.4:+79924240 | MS.gene80782:CDS |
| TAGCTATAGCTTCGCCACAG+AGG | 0.696399 | 4.4:-79931211 | None:intergenic |
| AGAAGAAACCTACCTGCTGG+CGG | 0.705987 | 4.4:-79930271 | None:intergenic |
| GGAGCTATGAGTTTGAGACA+AGG | 0.708450 | 4.4:+79929189 | MS.gene80782:CDS |
| CAGGAATTCCCAAAACCCCG+TGG | 0.722175 | 4.4:-79931394 | None:intergenic |
| ACATAAATAATATCGTCACG+TGG | 0.739907 | 4.4:-79933770 | None:intergenic |
| TCTGAGATGATGATCCTCTG+TGG | 0.740716 | 4.4:+79931197 | MS.gene80782:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 79924058 | 79933814 | 79924058 | ID=MS.gene80782 |
| chr4.4 | mRNA | 79924058 | 79933814 | 79924058 | ID=MS.gene80782.t1;Parent=MS.gene80782 |
| chr4.4 | exon | 79924058 | 79924273 | 79924058 | ID=MS.gene80782.t1.exon1;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79924058 | 79924273 | 79924058 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79924408 | 79924521 | 79924408 | ID=MS.gene80782.t1.exon2;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79924408 | 79924521 | 79924408 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79924611 | 79924688 | 79924611 | ID=MS.gene80782.t1.exon3;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79924611 | 79924688 | 79924611 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79924764 | 79924895 | 79924764 | ID=MS.gene80782.t1.exon4;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79924764 | 79924895 | 79924764 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79926384 | 79926605 | 79926384 | ID=MS.gene80782.t1.exon5;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79926384 | 79926605 | 79926384 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79927090 | 79927272 | 79927090 | ID=MS.gene80782.t1.exon6;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79927090 | 79927272 | 79927090 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79928762 | 79928824 | 79928762 | ID=MS.gene80782.t1.exon7;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79928762 | 79928824 | 79928762 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79928926 | 79929032 | 79928926 | ID=MS.gene80782.t1.exon8;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79928926 | 79929032 | 79928926 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79929113 | 79929262 | 79929113 | ID=MS.gene80782.t1.exon9;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79929113 | 79929262 | 79929113 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79930121 | 79930280 | 79930121 | ID=MS.gene80782.t1.exon10;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79930121 | 79930280 | 79930121 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79931080 | 79931475 | 79931080 | ID=MS.gene80782.t1.exon11;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79931080 | 79931475 | 79931080 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79932392 | 79932616 | 79932392 | ID=MS.gene80782.t1.exon12;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79932392 | 79932616 | 79932392 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| chr4.4 | exon | 79933689 | 79933814 | 79933689 | ID=MS.gene80782.t1.exon13;Parent=MS.gene80782.t1 |
| chr4.4 | CDS | 79933689 | 79933814 | 79933689 | ID=cds.MS.gene80782.t1;Parent=MS.gene80782.t1 |
| Gene Sequence |
| Protein sequence |
