Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84143.t1 | AES72443.1 | 98.4 | 63 | 1 | 0 | 1 | 63 | 1 | 63 | 9.60E-22 | 112.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84143.t1 | Q8L9T8 | 63.5 | 63 | 23 | 0 | 1 | 63 | 1 | 63 | 1.1e-10 | 66.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84143.t1 | G7J699 | 98.4 | 63 | 1 | 0 | 1 | 63 | 1 | 63 | 6.9e-22 | 112.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04926 | MS.gene84143 | 0.801324 | 9.50E-49 | -1.69E-46 |
| MS.gene04932 | MS.gene84143 | 0.8733 | 1.64E-67 | -1.69E-46 |
| MS.gene049549 | MS.gene84143 | 0.827185 | 1.80E-54 | -1.69E-46 |
| MS.gene050133 | MS.gene84143 | 0.819264 | 1.27E-52 | -1.69E-46 |
| MS.gene050329 | MS.gene84143 | 0.808215 | 3.46E-50 | -1.69E-46 |
| MS.gene050344 | MS.gene84143 | 0.800017 | 1.76E-48 | -1.69E-46 |
| MS.gene050369 | MS.gene84143 | 0.812662 | 3.79E-51 | -1.69E-46 |
| MS.gene050710 | MS.gene84143 | 0.825562 | 4.38E-54 | -1.69E-46 |
| MS.gene050720 | MS.gene84143 | 0.804194 | 2.43E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene84143 | 0.804788 | 1.83E-49 | -1.69E-46 |
| MS.gene051158 | MS.gene84143 | 0.8055 | 1.30E-49 | -1.69E-46 |
| MS.gene051385 | MS.gene84143 | 0.801577 | 8.43E-49 | -1.69E-46 |
| MS.gene051439 | MS.gene84143 | 0.8388 | 2.32E-57 | -1.69E-46 |
| MS.gene051525 | MS.gene84143 | -0.801395 | 9.19E-49 | -1.69E-46 |
| MS.gene051552 | MS.gene84143 | 0.828607 | 8.19E-55 | -1.69E-46 |
| MS.gene051838 | MS.gene84143 | -0.808565 | 2.91E-50 | -1.69E-46 |
| MS.gene051973 | MS.gene84143 | 0.804209 | 2.41E-49 | -1.69E-46 |
| MS.gene051996 | MS.gene84143 | 0.849173 | 3.83E-60 | -1.69E-46 |
| MS.gene052011 | MS.gene84143 | 0.808504 | 3.00E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene84143 | 0.814216 | 1.72E-51 | -1.69E-46 |
| MS.gene052223 | MS.gene84143 | 0.857904 | 1.19E-62 | -1.69E-46 |
| MS.gene05240 | MS.gene84143 | 0.861561 | 9.41E-64 | -1.69E-46 |
| MS.gene05244 | MS.gene84143 | 0.810511 | 1.11E-50 | -1.69E-46 |
| MS.gene052501 | MS.gene84143 | -0.816522 | 5.30E-52 | -1.69E-46 |
| MS.gene052503 | MS.gene84143 | -0.806526 | 7.88E-50 | -1.69E-46 |
| MS.gene052505 | MS.gene84143 | -0.819131 | 1.36E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene84143 | 0.804072 | 2.57E-49 | -1.69E-46 |
| MS.gene052702 | MS.gene84143 | 0.817688 | 2.90E-52 | -1.69E-46 |
| MS.gene052705 | MS.gene84143 | 0.813001 | 3.19E-51 | -1.69E-46 |
| MS.gene052706 | MS.gene84143 | 0.807534 | 4.82E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene84143 | -0.811704 | 6.13E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene84143 | -0.809736 | 1.63E-50 | -1.69E-46 |
| MS.gene053406 | MS.gene84143 | 0.830671 | 2.57E-55 | -1.69E-46 |
| MS.gene054107 | MS.gene84143 | -0.820711 | 5.94E-53 | -1.69E-46 |
| MS.gene054138 | MS.gene84143 | 0.824526 | 7.69E-54 | -1.69E-46 |
| MS.gene054147 | MS.gene84143 | 0.827421 | 1.58E-54 | -1.69E-46 |
| MS.gene054277 | MS.gene84143 | 0.867348 | 1.46E-65 | -1.69E-46 |
| MS.gene054742 | MS.gene84143 | 0.902026 | 1.48E-78 | -1.69E-46 |
| MS.gene054884 | MS.gene84143 | 0.813227 | 2.85E-51 | -1.69E-46 |
| MS.gene055215 | MS.gene84143 | 0.816655 | 4.95E-52 | -1.69E-46 |
| MS.gene055240 | MS.gene84143 | 0.81563 | 8.38E-52 | -1.69E-46 |
| MS.gene055289 | MS.gene84143 | -0.801074 | 1.07E-48 | -1.69E-46 |
| MS.gene056076 | MS.gene84143 | 0.81392 | 2.00E-51 | -1.69E-46 |
| MS.gene056330 | MS.gene84143 | 0.877868 | 4.45E-69 | -1.69E-46 |
| MS.gene056514 | MS.gene84143 | -0.814274 | 1.67E-51 | -1.69E-46 |
| MS.gene056590 | MS.gene84143 | 0.841876 | 3.64E-58 | -1.69E-46 |
| MS.gene056591 | MS.gene84143 | 0.840596 | 7.91E-58 | -1.69E-46 |
| MS.gene056633 | MS.gene84143 | -0.802121 | 6.52E-49 | -1.69E-46 |
| MS.gene056680 | MS.gene84143 | 0.854215 | 1.43E-61 | -1.69E-46 |
| MS.gene056688 | MS.gene84143 | 0.828615 | 8.15E-55 | -1.69E-46 |
| MS.gene056690 | MS.gene84143 | 0.815288 | 9.98E-52 | -1.69E-46 |
| MS.gene056691 | MS.gene84143 | 0.82839 | 9.23E-55 | -1.69E-46 |
| MS.gene056697 | MS.gene84143 | 0.821961 | 3.06E-53 | -1.69E-46 |
| MS.gene056748 | MS.gene84143 | 0.810187 | 1.31E-50 | -1.69E-46 |
| MS.gene056920 | MS.gene84143 | 0.900087 | 1.04E-77 | -1.69E-46 |
| MS.gene056922 | MS.gene84143 | 0.855928 | 4.54E-62 | -1.69E-46 |
| MS.gene056923 | MS.gene84143 | 0.842297 | 2.82E-58 | -1.69E-46 |
| MS.gene056924 | MS.gene84143 | 0.836459 | 9.25E-57 | -1.69E-46 |
| MS.gene056925 | MS.gene84143 | 0.824803 | 6.62E-54 | -1.69E-46 |
| MS.gene057048 | MS.gene84143 | -0.804661 | 1.94E-49 | -1.69E-46 |
| MS.gene057261 | MS.gene84143 | 0.833533 | 5.05E-56 | -1.69E-46 |
| MS.gene057544 | MS.gene84143 | 0.835261 | 1.86E-56 | -1.69E-46 |
| MS.gene057717 | MS.gene84143 | 0.806479 | 8.06E-50 | -1.69E-46 |
| MS.gene057856 | MS.gene84143 | -0.809827 | 1.56E-50 | -1.69E-46 |
| MS.gene058067 | MS.gene84143 | 0.801126 | 1.04E-48 | -1.69E-46 |
| MS.gene058381 | MS.gene84143 | 0.820612 | 6.26E-53 | -1.69E-46 |
| MS.gene058418 | MS.gene84143 | 0.828138 | 1.06E-54 | -1.69E-46 |
| MS.gene058444 | MS.gene84143 | -0.811203 | 7.87E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene84143 | 0.81796 | 2.51E-52 | -1.69E-46 |
| MS.gene05926 | MS.gene84143 | 0.81857 | 1.83E-52 | -1.69E-46 |
| MS.gene059328 | MS.gene84143 | 0.816061 | 6.71E-52 | -1.69E-46 |
| MS.gene059512 | MS.gene84143 | -0.80731 | 5.38E-50 | -1.69E-46 |
| MS.gene059891 | MS.gene84143 | 0.848884 | 4.61E-60 | -1.69E-46 |
| MS.gene059892 | MS.gene84143 | 0.842511 | 2.47E-58 | -1.69E-46 |
| MS.gene059955 | MS.gene84143 | 0.818796 | 1.63E-52 | -1.69E-46 |
| MS.gene060087 | MS.gene84143 | 0.896161 | 4.81E-76 | -1.69E-46 |
| MS.gene060358 | MS.gene84143 | 0.815054 | 1.12E-51 | -1.69E-46 |
| MS.gene060405 | MS.gene84143 | 0.808035 | 3.77E-50 | -1.69E-46 |
| MS.gene061038 | MS.gene84143 | 0.809648 | 1.71E-50 | -1.69E-46 |
| MS.gene061092 | MS.gene84143 | 0.83803 | 3.66E-57 | -1.69E-46 |
| MS.gene061094 | MS.gene84143 | 0.844606 | 6.81E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene55776 | MS.gene84143 | PPI |
| MS.gene06050 | MS.gene84143 | PPI |
| MS.gene048906 | MS.gene84143 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84143.t1 | MTR_3g090830 | 98.413 | 63 | 1 | 0 | 1 | 63 | 1 | 63 | 3.94e-38 | 120 |
| MS.gene84143.t1 | MTR_1g028610 | 76.271 | 59 | 12 | 1 | 7 | 63 | 4 | 62 | 2.29e-18 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84143.t1 | AT2G46330 | 63.462 | 52 | 19 | 0 | 12 | 63 | 11 | 62 | 9.80e-19 | 72.4 |
| MS.gene84143.t1 | AT3G61640 | 56.667 | 60 | 26 | 0 | 4 | 63 | 3 | 62 | 1.35e-17 | 69.3 |
| MS.gene84143.t1 | AT5G53250 | 58.182 | 55 | 23 | 0 | 9 | 63 | 9 | 63 | 2.23e-15 | 63.5 |
| MS.gene84143.t1 | AT5G24105 | 63.492 | 63 | 23 | 0 | 1 | 63 | 1 | 63 | 4.83e-11 | 52.4 |
Find 20 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTTTCATGTGCTTCATTC+AGG | 0.175879 | 3.1:-72771304 | MS.gene84143:CDS |
| TGTTCTAATGCTTGTGGCTT+TGG | 0.292333 | 3.1:-72770466 | MS.gene84143:CDS |
| TTCAGGGTGGTTGCCTTCCT+TGG | 0.296499 | 3.1:-72771287 | MS.gene84143:CDS |
| GATAAATTAATGAATGATAT+AGG | 0.396044 | 3.1:+72770437 | None:intergenic |
| GAATATTTACCATCGCTTGT+AGG | 0.408971 | 3.1:+72771203 | None:intergenic |
| TGTTTCATGTGCTTCATTCA+GGG | 0.448362 | 3.1:-72771303 | MS.gene84143:CDS |
| TTACCATCGCTTGTAGGAGC+AGG | 0.455603 | 3.1:+72771209 | None:intergenic |
| AGGAGCAGGGGCAGGTGCAA+TGG | 0.532358 | 3.1:+72771223 | None:intergenic |
| TTATGCAACACTTATTTCTG+TGG | 0.538882 | 3.1:-72771258 | MS.gene84143:CDS |
| TACCATCGCTTGTAGGAGCA+GGG | 0.542274 | 3.1:+72771210 | None:intergenic |
| AAATAAGTGTTGCATAAATG+AGG | 0.548157 | 3.1:+72771264 | None:intergenic |
| TGCATGTGTTCTAATGCTTG+TGG | 0.551000 | 3.1:-72770472 | MS.gene84143:CDS |
| TCGCTTGTAGGAGCAGGGGC+AGG | 0.566771 | 3.1:+72771215 | None:intergenic |
| TGCATAAATGAGGCCAAGGA+AGG | 0.567934 | 3.1:+72771274 | None:intergenic |
| TTAGAACACATGCAATCGCT+TGG | 0.574241 | 3.1:+72770481 | None:intergenic |
| TGCAATCGCTTGGTCAATTG+TGG | 0.581446 | 3.1:+72770491 | None:intergenic |
| GTGTTGCATAAATGAGGCCA+AGG | 0.582795 | 3.1:+72771270 | None:intergenic |
| GCCCCTGCTCCTACAAGCGA+TGG | 0.595947 | 3.1:-72771212 | MS.gene84143:intron |
| ACCATCGCTTGTAGGAGCAG+GGG | 0.636251 | 3.1:+72771211 | None:intergenic |
| TTCATGTGCTTCATTCAGGG+TGG | 0.668781 | 3.1:-72771300 | MS.gene84143:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAAATTGAAAATCAAATA+TGG | + | chr3.1:72771148-72771167 | None:intergenic | 15.0% |
| !! | TAATGATTATAAAACAAATG+TGG | - | chr3.1:72770983-72771002 | MS.gene84143:intron | 15.0% |
| !! | TGAAAAATAAATTAAAATGG+AGG | + | chr3.1:72770664-72770683 | None:intergenic | 15.0% |
| !!! | AATCATTTCATAAATCTTTT+TGG | - | chr3.1:72770858-72770877 | MS.gene84143:intron | 15.0% |
| !!! | TTTATTTTCAGAGATTAAAT+TGG | + | chr3.1:72770704-72770723 | None:intergenic | 15.0% |
| !! | AAAGTGATGAAACATTAATA+AGG | + | chr3.1:72770604-72770623 | None:intergenic | 20.0% |
| !! | AAGTGATGAAACATTAATAA+GGG | + | chr3.1:72770603-72770622 | None:intergenic | 20.0% |
| !! | GCTAAAAGTTAAGTAAAATT+TGG | + | chr3.1:72771065-72771084 | None:intergenic | 20.0% |
| !!! | AAAACACCTTATACTTTTTA+AGG | - | chr3.1:72770900-72770919 | MS.gene84143:intron | 20.0% |
| !!! | AATTGTTGTTTATAATATGC+AGG | - | chr3.1:72771235-72771254 | MS.gene84143:CDS | 20.0% |
| !!! | ATTAAGCCTTAAAAAGTATA+AGG | + | chr3.1:72770909-72770928 | None:intergenic | 20.0% |
| ! | TAATATCCATATAAACGGTT+AGG | - | chr3.1:72771008-72771027 | MS.gene84143:intron | 25.0% |
| ! | TCTTTATGAGCATTATTGAT+AGG | - | chr3.1:72771186-72771205 | MS.gene84143:intron | 25.0% |
| ! | TGGACTAATATCCATATAAA+CGG | - | chr3.1:72771003-72771022 | MS.gene84143:intron | 25.0% |
| !! | AAATAAGTGTTGCATAAATG+AGG | + | chr3.1:72770490-72770509 | None:intergenic | 25.0% |
| !! | ATCAAATATGGAGATGTTTT+TGG | + | chr3.1:72771136-72771155 | None:intergenic | 25.0% |
| !! | GATTAAATTGGTGATTCATT+TGG | + | chr3.1:72770692-72770711 | None:intergenic | 25.0% |
| !!! | AAACTCACATATATTTTGAG+AGG | + | chr3.1:72770941-72770960 | None:intergenic | 25.0% |
| !!! | CTTTCAATCACATTAGTTTT+CGG | + | chr3.1:72770829-72770848 | None:intergenic | 25.0% |
| GCTCATAAAGAATAATAGCA+TGG | + | chr3.1:72771177-72771196 | None:intergenic | 30.0% | |
| !! | TTATGCAACACTTATTTCTG+TGG | - | chr3.1:72770493-72770512 | MS.gene84143:CDS | 30.0% |
| GAATATTTACCATCGCTTGT+AGG | + | chr3.1:72770551-72770570 | None:intergenic | 35.0% | |
| GTTCTTCCTAACCGTTTATA+TGG | + | chr3.1:72771017-72771036 | None:intergenic | 35.0% | |
| TGTTTCATGTGCTTCATTCA+GGG | - | chr3.1:72770448-72770467 | MS.gene84143:CDS | 35.0% | |
| AAGATTGTTCCAACAAACCC+AGG | + | chr3.1:72771039-72771058 | None:intergenic | 40.0% | |
| ATAAACGGTTAGGAAGAACC+TGG | - | chr3.1:72771018-72771037 | MS.gene84143:intron | 40.0% | |
| CTGTTTCATGTGCTTCATTC+AGG | - | chr3.1:72770447-72770466 | MS.gene84143:CDS | 40.0% | |
| TAAACGGTTAGGAAGAACCT+GGG | - | chr3.1:72771019-72771038 | MS.gene84143:intron | 40.0% | |
| TCCCATTCCCTAGAACTAAA+GGG | - | chr3.1:72771104-72771123 | MS.gene84143:intron | 40.0% | |
| TGCATGTGTTCTAATGCTTG+TGG | - | chr3.1:72771279-72771298 | MS.gene84143:CDS | 40.0% | |
| TGTTCTAATGCTTGTGGCTT+TGG | - | chr3.1:72771285-72771304 | MS.gene84143:CDS | 40.0% | |
| TTAGAACACATGCAATCGCT+TGG | + | chr3.1:72771273-72771292 | None:intergenic | 40.0% | |
| !! | AACCCTTTAGTTCTAGGGAA+TGG | + | chr3.1:72771109-72771128 | None:intergenic | 40.0% |
| !! | ACCCTTTAGTTCTAGGGAAT+GGG | + | chr3.1:72771108-72771127 | None:intergenic | 40.0% |
| GTCCCATTCCCTAGAACTAA+AGG | - | chr3.1:72771103-72771122 | MS.gene84143:intron | 45.0% | |
| GTGTTGCATAAATGAGGCCA+AGG | + | chr3.1:72770484-72770503 | None:intergenic | 45.0% | |
| TAGGAAGAACCTGGGTTTGT+TGG | - | chr3.1:72771027-72771046 | MS.gene84143:intron | 45.0% | |
| TCCCTAGAACTAAAGGGTTG+TGG | - | chr3.1:72771110-72771129 | MS.gene84143:intron | 45.0% | |
| TGCAATCGCTTGGTCAATTG+TGG | + | chr3.1:72771263-72771282 | None:intergenic | 45.0% | |
| TGCATAAATGAGGCCAAGGA+AGG | + | chr3.1:72770480-72770499 | None:intergenic | 45.0% | |
| TTCATGTGCTTCATTCAGGG+TGG | - | chr3.1:72770451-72770470 | MS.gene84143:CDS | 45.0% | |
| !! | GCCACAACCCTTTAGTTCTA+GGG | + | chr3.1:72771114-72771133 | None:intergenic | 45.0% |
| !! | TAGTTCTAGGGAATGGGACA+TGG | + | chr3.1:72771102-72771121 | None:intergenic | 45.0% |
| !! | TATTGAAAAATAAATTAAAA+TGG | + | chr3.1:72770667-72770686 | None:intergenic | 5.0% |
| TACCATCGCTTGTAGGAGCA+GGG | + | chr3.1:72770544-72770563 | None:intergenic | 50.0% | |
| TTACCATCGCTTGTAGGAGC+AGG | + | chr3.1:72770545-72770564 | None:intergenic | 50.0% | |
| !! | GGCCACAACCCTTTAGTTCT+AGG | + | chr3.1:72771115-72771134 | None:intergenic | 50.0% |
| ACCATCGCTTGTAGGAGCAG+GGG | + | chr3.1:72770543-72770562 | None:intergenic | 55.0% | |
| TTCAGGGTGGTTGCCTTCCT+TGG | - | chr3.1:72770464-72770483 | MS.gene84143:CDS | 55.0% | |
| AGGAGCAGGGGCAGGTGCAA+TGG | + | chr3.1:72770531-72770550 | None:intergenic | 65.0% | |
| GCCCCTGCTCCTACAAGCGA+TGG | - | chr3.1:72770539-72770558 | MS.gene84143:intron | 65.0% | |
| TCGCTTGTAGGAGCAGGGGC+AGG | + | chr3.1:72770539-72770558 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 72770443 | 72771330 | 72770443 | ID=MS.gene84143 |
| chr3.1 | mRNA | 72770443 | 72771330 | 72770443 | ID=MS.gene84143.t1;Parent=MS.gene84143 |
| chr3.1 | exon | 72771213 | 72771330 | 72771213 | ID=MS.gene84143.t1.exon1;Parent=MS.gene84143.t1 |
| chr3.1 | CDS | 72771213 | 72771330 | 72771213 | ID=cds.MS.gene84143.t1;Parent=MS.gene84143.t1 |
| chr3.1 | exon | 72770443 | 72770516 | 72770443 | ID=MS.gene84143.t1.exon2;Parent=MS.gene84143.t1 |
| chr3.1 | CDS | 72770443 | 72770516 | 72770443 | ID=cds.MS.gene84143.t1;Parent=MS.gene84143.t1 |
| Gene Sequence |
| Protein sequence |
