AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023125.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 11 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTAAATCTGAAGCTTTA+AGG 0.110020 4:+81143179 MsG0480023125.01.T01:CDS
CCAACTCTCCACAGTTGATA+AGG 0.434883 4:-81143084 None:intergenic
CTCAGAACTCCAAGTGCAGC+TGG 0.436184 4:+81143125 MsG0480023125.01.T01:CDS
ACTGGTGCACCAGCTGCACT+TGG 0.490099 4:-81143134 None:intergenic
CTCTCCACAGTTGATAAGGA+TGG 0.512074 4:-81143080 None:intergenic
CCTTATCAACTGTGGAGAGT+TGG 0.513468 4:+81143084 MsG0480023125.01.T01:CDS
AGTATCATCAAGATCTTGAC+TGG 0.560635 4:-81143152 None:intergenic
TCTCCACAGTTGATAAGGAT+GGG 0.596020 4:-81143079 None:intergenic
TGGCTGAAGATGGTTCATCA+AGG 0.647563 4:+81143032 None:intergenic
AGACCCATCCTTATCAACTG+TGG 0.653046 4:+81143076 MsG0480023125.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGTATCATCAAGATCTTGAC+TGG - Chr4:81143155-81143174 None:intergenic 35.0%
!! TCTCCACAGTTGATAAGGAT+GGG - Chr4:81143082-81143101 None:intergenic 40.0%
AGACCCATCCTTATCAACTG+TGG + Chr4:81143076-81143095 MsG0480023125.01.T01:CDS 45.0%
CCTTATCAACTGTGGAGAGT+TGG + Chr4:81143084-81143103 MsG0480023125.01.T01:CDS 45.0%
! CCAACTCTCCACAGTTGATA+AGG - Chr4:81143087-81143106 None:intergenic 45.0%
! GATAAGGATGGGTCTTTTGC+TGG - Chr4:81143071-81143090 None:intergenic 45.0%
!! CTCTCCACAGTTGATAAGGA+TGG - Chr4:81143083-81143102 None:intergenic 45.0%
!! GGTCTTTTGCTGGCTGAAAA+GGG - Chr4:81143061-81143080 None:intergenic 45.0%
!! GGGTCTTTTGCTGGCTGAAA+AGG - Chr4:81143062-81143081 None:intergenic 50.0%
CTCAGAACTCCAAGTGCAGC+TGG + Chr4:81143125-81143144 MsG0480023125.01.T01:CDS 55.0%
ACTGGTGCACCAGCTGCACT+TGG - Chr4:81143137-81143156 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 81143041 81143199 81143041 ID=MsG0480023125.01;Name=MsG0480023125.01
Chr4 mRNA 81143041 81143199 81143041 ID=MsG0480023125.01.T01;Parent=MsG0480023125.01;Name=MsG0480023125.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|0|1|0|52
Chr4 exon 81143041 81143199 81143041 ID=MsG0480023125.01.T01:exon:4149;Parent=MsG0480023125.01.T01
Chr4 CDS 81143041 81143199 81143041 ID=MsG0480023125.01.T01:cds;Parent=MsG0480023125.01.T01
Gene Sequence

>MsG0480023125.01.T01

ATGGTTCATCAAGGAAACCCTTTTCAGCCAGCAAAAGACCCATCCTTATCAACTGTGGAGAGTTGGTTTCAAAGTCAAAGTGTTCTCAGAACTCCAAGTGCAGCTGGTGCACCAGTCAAGATCTTGATGATACTTGCTTCTCTAAATCTGAAGCTTTAA

Protein sequence

>MsG0480023125.01.T01

MVHQGNPFQPAKDPSLSTVESWFQSQSVLRTPSAAGAPVKILMILASLNLKL*