AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038573.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038573.01.T01 MTR_7g057160 96.407 167 6 0 1 167 1 167 8.19e-119 333
MsG0780038573.01.T01 MTR_3g436010 47.260 146 71 1 16 161 169 308 3.68e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038573.01.T01 AT3G17609 60.000 130 47 2 39 167 22 147 6.23e-44 142
MsG0780038573.01.T01 AT3G17609 61.157 121 42 2 48 167 2 118 4.06e-41 134
MsG0780038573.01.T01 AT3G17609 74.444 90 22 1 79 167 44 133 4.40e-40 132
MsG0780038573.01.T01 AT3G17609 74.444 90 22 1 79 167 43 132 4.56e-40 132
MsG0780038573.01.T01 AT5G11260 48.175 137 54 3 31 161 26 151 4.54e-33 115
MsG0780038573.01.T01 AT5G11260 48.120 133 52 3 31 157 26 147 1.74e-30 109

Find 40 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTTACATTGCTTTGATTA+TGG 0.188466 7:-48998729 None:intergenic
TGAGATACGTTCTTCTAAAT+TGG 0.194375 7:-48999302 None:intergenic
CTTCATCTTCATCTTCTTCA+TGG 0.213050 7:+48997603 MsG0780038573.01.T01:CDS
TCTTCCACGGCGGCGCTTGC+CGG 0.245919 7:-48998776 None:intergenic
CTTCCACGGCGGCGCTTGCC+GGG 0.275869 7:-48998775 None:intergenic
CAAATAGGTTGCTAAGGAAT+AGG 0.306771 7:+48999194 MsG0780038573.01.T01:intron
CCTTCATTGCTCATCAACTT+TGG 0.354625 7:-48999496 None:intergenic
CTTCATTGCTCATCAACTTT+GGG 0.354751 7:-48999495 None:intergenic
CATTGTGTACCTTCCACATC+TGG 0.376399 7:-48998667 None:intergenic
TTCCTTCATCTCAGTATCAT+TGG 0.395557 7:-48997561 None:intergenic
TCGGAGCATGGCGTTCTCAT+TGG 0.400151 7:-48999332 None:intergenic
ATGAAGGAAGCTGCAACAAC+TGG 0.409191 7:+48997575 MsG0780038573.01.T01:CDS
TTTGATTTGTTAACTCAGAA+TGG 0.409238 7:-48998699 None:intergenic
CGTCTATGTTCCTTGTCTGC+AGG 0.409298 7:-48998802 None:intergenic
CCTATGCCTCCACATAGATT+TGG 0.415132 7:+48997638 MsG0780038573.01.T01:CDS
AAATAGGTTGCTAAGGAATA+GGG 0.428080 7:+48999195 MsG0780038573.01.T01:intron
CTTCATCTTCTTCATGGAGT+AGG 0.466765 7:+48997609 MsG0780038573.01.T01:CDS
CAACTTCAAGCAGGCTTCCC+CGG 0.470731 7:+48998757 MsG0780038573.01.T01:CDS
GTCTATGTTCCTTGTCTGCA+GGG 0.478530 7:-48998801 None:intergenic
AGCCAATGATACTGAGATGA+AGG 0.496590 7:+48997559 MsG0780038573.01.T01:CDS
TGTCTGCAGGGTTTCTTCCA+CGG 0.500164 7:-48998789 None:intergenic
CCAAAGTTGATGAGCAATGA+AGG 0.501104 7:+48999496 MsG0780038573.01.T01:CDS
TTATGTTACCTTTCGGAGCA+TGG 0.517776 7:-48999344 None:intergenic
TTCCACGGCGGCGCTTGCCG+GGG 0.522944 7:-48998774 None:intergenic
CCAAATCTATGTGGAGGCAT+AGG 0.526031 7:-48997638 None:intergenic
AGGAACATAGACGTCTCAAA+AGG 0.535376 7:+48998812 MsG0780038573.01.T01:CDS
GTGTTACAAATAGGTTGCTA+AGG 0.552163 7:+48999188 MsG0780038573.01.T01:intron
TGGAAGAAACCCTGCAGACA+AGG 0.552255 7:+48998792 MsG0780038573.01.T01:CDS
ACCGATGCTCAACTTCAAGC+AGG 0.560214 7:+48998748 MsG0780038573.01.T01:CDS
CATGAAGAAGATGAAGATGA+AGG 0.570746 7:-48997602 None:intergenic
GTTGCTAAGGAATAGGGTCT+CGG 0.571634 7:+48999201 MsG0780038573.01.T01:CDS
AATCTATGTGGAGGCATAGG+AGG 0.581180 7:-48997635 None:intergenic
GCCTGCTTGAAGTTGAGCAT+CGG 0.588947 7:-48998749 None:intergenic
TTCCCCGGCAAGCGCCGCCG+TGG 0.608180 7:+48998772 MsG0780038573.01.T01:CDS
TGAGAACGCCATGCTCCGAA+AGG 0.615500 7:+48999336 MsG0780038573.01.T01:CDS
CACTTACCAAATCTATGTGG+AGG 0.634191 7:-48997644 None:intergenic
TCTGTTCGAAGTTCCAGATG+TGG 0.639704 7:+48998654 MsG0780038573.01.T01:CDS
TGACACTTACCAAATCTATG+TGG 0.647225 7:-48997647 None:intergenic
CTGCAGGGTTTCTTCCACGG+CGG 0.647406 7:-48998786 None:intergenic
TTCGAAGTTCCAGATGTGGA+AGG 0.651950 7:+48998658 MsG0780038573.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAATTGTAATATGTAT+CGG - Chr7:48999011-48999030 None:intergenic 10.0%
!! TTGTTAAATAAAAATTTAAC+GGG - Chr7:48998059-48998078 None:intergenic 10.0%
!! AAGAATATTAAGTATATTGT+TGG + Chr7:48997909-48997928 MsG0780038573.01.T01:intron 15.0%
!! AATGTCAAATTAAAATTTGA+AGG - Chr7:48998517-48998536 None:intergenic 15.0%
!! ATGTCAAATTAAAATTTGAA+GGG - Chr7:48998516-48998535 None:intergenic 15.0%
!! GGTATATATATAATATAAGT+TGG + Chr7:48997973-48997992 MsG0780038573.01.T01:intron 15.0%
!! AAAACAAGTCAAGTTTAATA+TGG - Chr7:48998560-48998579 None:intergenic 20.0%
!! AACTACATACAATTCAAATT+TGG + Chr7:48998350-48998369 MsG0780038573.01.T01:intron 20.0%
!! AATGCAAAATTGTTACTATT+AGG + Chr7:48998455-48998474 MsG0780038573.01.T01:intron 20.0%
!! TCAAAATTTGCATTGAATTT+CGG + Chr7:48998274-48998293 MsG0780038573.01.T01:intron 20.0%
!! TCAAATTGTTGATGTAAAAA+CGG - Chr7:48998134-48998153 None:intergenic 20.0%
!!! ACAAGTTGTTTTTAAATGAT+CGG - Chr7:48997938-48997957 None:intergenic 20.0%
!!! TAATATGGACTAAATTGAAT+TGG - Chr7:48998545-48998564 None:intergenic 20.0%
! AACTTTGTGATATGATATTG+TGG - Chr7:48998171-48998190 None:intergenic 25.0%
! ACTTGTTTCATGAAAAGTAA+AGG + Chr7:48997952-48997971 MsG0780038573.01.T01:intron 25.0%
! ATAACATCATGTTGATCAAA+TGG + Chr7:48999425-48999444 MsG0780038573.01.T01:intron 25.0%
! ATATGCGTGTGTATATTATA+AGG - Chr7:48998014-48998033 None:intergenic 25.0%
! TACACACGCATATATTAAAT+TGG + Chr7:48998021-48998040 MsG0780038573.01.T01:intron 25.0%
! TATGAAGAATCTTGTAAGTT+TGG - Chr7:48997833-48997852 None:intergenic 25.0%
! TATGCGTGTGTATATTATAA+GGG - Chr7:48998013-48998032 None:intergenic 25.0%
! TCAAATGGAAATTTATTCGA+TGG + Chr7:48999440-48999459 MsG0780038573.01.T01:intron 25.0%
!! AAATAAAAGACCTTAACTTG+AGG - Chr7:48997733-48997752 None:intergenic 25.0%
!! AAATGACTTGTGTTACAAAT+AGG + Chr7:48999179-48999198 MsG0780038573.01.T01:intron 25.0%
!! CATATTTTATCCTCAAGTTA+AGG + Chr7:48997720-48997739 MsG0780038573.01.T01:intron 25.0%
!!! ATATAAGTTGGTCCATATTA+AGG + Chr7:48997985-48998004 MsG0780038573.01.T01:intron 25.0%
!!! TATAAGTTGGTCCATATTAA+GGG + Chr7:48997986-48998005 MsG0780038573.01.T01:intron 25.0%
!!! TTTGATTTGTTAACTCAGAA+TGG - Chr7:48998702-48998721 None:intergenic 25.0%
AAATAGGTTGCTAAGGAATA+GGG + Chr7:48999195-48999214 MsG0780038573.01.T01:intron 30.0%
AATAGTGACTATAGAGTGTA+AGG - Chr7:48998872-48998891 None:intergenic 30.0%
ACATTAACATGTGCTAACAT+TGG - Chr7:48998376-48998395 None:intergenic 30.0%
ATGATTATCGATCATTGTGA+AGG - Chr7:48998200-48998219 None:intergenic 30.0%
GTCTGACTTTACTTATCTTT+CGG + Chr7:48999124-48999143 MsG0780038573.01.T01:intron 30.0%
GTTCAGATTATGTTACCTTT+CGG - Chr7:48999354-48999373 None:intergenic 30.0%
TGAGATACGTTCTTCTAAAT+TGG - Chr7:48999305-48999324 None:intergenic 30.0%
!! ATAAGTTGGTCCATATTAAG+GGG + Chr7:48997987-48998006 MsG0780038573.01.T01:intron 30.0%
!!! CGTACACTTTTTTTCTTTCA+CGG - Chr7:48999237-48999256 None:intergenic 30.0%
!!! GTACACTTTTTTTCTTTCAC+GGG - Chr7:48999236-48999255 None:intergenic 30.0%
ATAGACGTCTCAAAAGGTAA+GGG + Chr7:48998818-48998837 MsG0780038573.01.T01:intron 35.0%
ATTATAAGGGCCCCTTAATA+TGG - Chr7:48998000-48998019 None:intergenic 35.0%
ATTATCGATCATTGTGAAGG+TGG - Chr7:48998197-48998216 None:intergenic 35.0%
CAAATAGGTTGCTAAGGAAT+AGG + Chr7:48999194-48999213 MsG0780038573.01.T01:intron 35.0%
CATGAAGAAGATGAAGATGA+AGG - Chr7:48997605-48997624 None:intergenic 35.0%
CGGTTACATTGCTTTGATTA+TGG - Chr7:48998732-48998751 None:intergenic 35.0%
CTCAAACGATTGTTGAACTT+CGG + Chr7:48999059-48999078 MsG0780038573.01.T01:intron 35.0%
CTTCATCTTCATCTTCTTCA+TGG + Chr7:48997603-48997622 MsG0780038573.01.T01:CDS 35.0%
GTGTTACAAATAGGTTGCTA+AGG + Chr7:48999188-48999207 MsG0780038573.01.T01:intron 35.0%
TATTGATAGGCTCTTGACTT+AGG - Chr7:48997776-48997795 None:intergenic 35.0%
TGACACTTACCAAATCTATG+TGG - Chr7:48997650-48997669 None:intergenic 35.0%
TTCCTTCATCTCAGTATCAT+TGG - Chr7:48997564-48997583 None:intergenic 35.0%
!! TTCGATGGTTTTACTTGTAC+AGG + Chr7:48999455-48999474 MsG0780038573.01.T01:intron 35.0%
AGCCAATGATACTGAGATGA+AGG + Chr7:48997559-48997578 MsG0780038573.01.T01:CDS 40.0%
AGGAACATAGACGTCTCAAA+AGG + Chr7:48998812-48998831 MsG0780038573.01.T01:CDS 40.0%
CACTTACCAAATCTATGTGG+AGG - Chr7:48997647-48997666 None:intergenic 40.0%
CATAGACGTCTCAAAAGGTA+AGG + Chr7:48998817-48998836 MsG0780038573.01.T01:intron 40.0%
CTTCATCTTCTTCATGGAGT+AGG + Chr7:48997609-48997628 MsG0780038573.01.T01:CDS 40.0%
TTATGTTACCTTTCGGAGCA+TGG - Chr7:48999347-48999366 None:intergenic 40.0%
! TTTTTCTTTCACGGGCTTGT+TGG - Chr7:48999228-48999247 None:intergenic 40.0%
!! TTTTCTTTCACGGGCTTGTT+GGG - Chr7:48999227-48999246 None:intergenic 40.0%
AGATCTTCATCGCTATCGTC+TGG - Chr7:48998637-48998656 None:intergenic 45.0%
ATGAAGGAAGCTGCAACAAC+TGG + Chr7:48997575-48997594 MsG0780038573.01.T01:CDS 45.0%
CATTGTGTACCTTCCACATC+TGG - Chr7:48998670-48998689 None:intergenic 45.0%
CCTATGCCTCCACATAGATT+TGG + Chr7:48997638-48997657 MsG0780038573.01.T01:CDS 45.0%
GGTGACAGTTGGATCTAGAT+TGG - Chr7:48998304-48998323 None:intergenic 45.0%
GTCTATGTTCCTTGTCTGCA+GGG - Chr7:48998804-48998823 None:intergenic 45.0%
GTTGCTAAGGAATAGGGTCT+CGG + Chr7:48999201-48999220 MsG0780038573.01.T01:CDS 45.0%
TAGATCCAACTGTCACCTGA+GGG + Chr7:48998307-48998326 MsG0780038573.01.T01:intron 45.0%
TCTGTTCGAAGTTCCAGATG+TGG + Chr7:48998654-48998673 MsG0780038573.01.T01:CDS 45.0%
TTCGAAGTTCCAGATGTGGA+AGG + Chr7:48998658-48998677 MsG0780038573.01.T01:CDS 45.0%
! AATCTATGTGGAGGCATAGG+AGG - Chr7:48997638-48997657 None:intergenic 45.0%
! CCAAATCTATGTGGAGGCAT+AGG - Chr7:48997641-48997660 None:intergenic 45.0%
!! AAACTAATTAAAAAAAAAAT+TGG - Chr7:48997676-48997695 None:intergenic 5.0%
!! ATTGTTAAATAAAAATTTAA+CGG - Chr7:48998060-48998079 None:intergenic 5.0%
!!! TTTTTTAATTAAATATTGAT+AGG - Chr7:48997789-48997808 None:intergenic 5.0%
ACCGATGCTCAACTTCAAGC+AGG + Chr7:48998748-48998767 MsG0780038573.01.T01:CDS 50.0%
CGTCTATGTTCCTTGTCTGC+AGG - Chr7:48998805-48998824 None:intergenic 50.0%
CTAGATCCAACTGTCACCTG+AGG + Chr7:48998306-48998325 MsG0780038573.01.T01:intron 50.0%
GCCTGCTTGAAGTTGAGCAT+CGG - Chr7:48998752-48998771 None:intergenic 50.0%
TGGAAGAAACCCTGCAGACA+AGG + Chr7:48998792-48998811 MsG0780038573.01.T01:CDS 50.0%
TGTCTGCAGGGTTTCTTCCA+CGG - Chr7:48998792-48998811 None:intergenic 50.0%
CAACTTCAAGCAGGCTTCCC+CGG + Chr7:48998757-48998776 MsG0780038573.01.T01:CDS 55.0%
TCACTCCCTCAGGTGACAGT+TGG - Chr7:48998315-48998334 None:intergenic 55.0%
TCGGAGCATGGCGTTCTCAT+TGG - Chr7:48999335-48999354 None:intergenic 55.0%
TCTCGTGCATTCACTCCCTC+AGG - Chr7:48998325-48998344 None:intergenic 55.0%
TGAGAACGCCATGCTCCGAA+AGG + Chr7:48999336-48999355 MsG0780038573.01.T01:CDS 55.0%
CTGCAGGGTTTCTTCCACGG+CGG - Chr7:48998789-48998808 None:intergenic 60.0%
TCTTCCACGGCGGCGCTTGC+CGG - Chr7:48998779-48998798 None:intergenic 70.0%
CTTCCACGGCGGCGCTTGCC+GGG - Chr7:48998778-48998797 None:intergenic 75.0%
TTCCACGGCGGCGCTTGCCG+GGG - Chr7:48998777-48998796 None:intergenic 75.0%
!! TTCCCCGGCAAGCGCCGCCG+TGG + Chr7:48998772-48998791 MsG0780038573.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr7 gene 48997545 48999515 48997545 ID=MsG0780038573.01;Name=MsG0780038573.01
Chr7 mRNA 48997545 48999515 48997545 ID=MsG0780038573.01.T01;Parent=MsG0780038573.01;Name=MsG0780038573.01.T01;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|1|4|0|168
Chr7 exon 48997545 48997659 48997545 ID=MsG0780038573.01.T01:exon:6540;Parent=MsG0780038573.01.T01
Chr7 exon 48998638 48998833 48998638 ID=MsG0780038573.01.T01:exon:6541;Parent=MsG0780038573.01.T01
Chr7 exon 48999201 48999357 48999201 ID=MsG0780038573.01.T01:exon:6542;Parent=MsG0780038573.01.T01
Chr7 exon 48999477 48999515 48999477 ID=MsG0780038573.01.T01:exon:6543;Parent=MsG0780038573.01.T01
Chr7 CDS 48997545 48997659 48997545 ID=MsG0780038573.01.T01:cds;Parent=MsG0780038573.01.T01
Chr7 CDS 48998638 48998833 48998638 ID=MsG0780038573.01.T01:cds;Parent=MsG0780038573.01.T01
Chr7 CDS 48999201 48999357 48999201 ID=MsG0780038573.01.T01:cds;Parent=MsG0780038573.01.T01
Chr7 CDS 48999477 48999515 48999477 ID=MsG0780038573.01.T01:cds;Parent=MsG0780038573.01.T01
Gene Sequence

>MsG0780038573.01.T01

ATGTCTCTTTCAAGAGCCAATGATACTGAGATGAAGGAAGCTGCAACAACTGGAGCACCTTCATCTTCATCTTCTTCATGGAGTAGGTTACCTCCTATGCCTCCACATAGATTTGACGATAGCGATGAAGATCTGTTCGAAGTTCCAGATGTGGAAGGTACACAATGTCAAAGTGTCCATTCTGAGTTAACAAATCAAAATAGTAACCATAATCAAAGCAATGTAACCGATGCTCAACTTCAAGCAGGCTTCCCCGGCAAGCGCCGCCGTGGAAGAAACCCTGCAGACAAGGAACATAGACGTCTCAAAAGGTTGCTAAGGAATAGGGTCTCGGCCCAACAAGCCCGTGAAAGAAAAAAAGTGTACGTGAATGACTTAGAATCAAGAGCTAAAGAGTTTGAAGATAAGAACTCCAATTTAGAAGAACGTATCTCAACTTTGACCAATGAGAACGCCATGCTCCGAAAGGTTCTTATGAACACTAGACCCAAAGTTGATGAGCAATGA

Protein sequence

>MsG0780038573.01.T01

MSLSRANDTEMKEAATTGAPSSSSSSWSRLPPMPPHRFDDSDEDLFEVPDVEGTQCQSVHSELTNQNSNHNQSNVTDAQLQAGFPGKRRRGRNPADKEHRRLKRLLRNRVSAQQARERKKVYVNDLESRAKEFEDKNSNLEERISTLTNENAMLRKVLMNTRPKVDEQ*