Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025573.01.T01 | AES95894.1 | 98.438 | 128 | 2 | 0 | 1 | 128 | 2 | 129 | 3.09E-86 | 257 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025573.01.T01 | Q9LSK9 | 55.906 | 127 | 54 | 2 | 2 | 128 | 1 | 125 | 6.35E-42 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047967.01 | MsG0580025573.01 | -0.819102 | 1.385121e-52 | 1.056024e-49 |
| MsG0080048692.01 | MsG0580025573.01 | -0.800842 | 1.192137e-48 | 5.608206e-46 |
| MsG0080048694.01 | MsG0580025573.01 | -0.822439 | 2.367550e-53 | 1.981743e-50 |
| MsG0080048695.01 | MsG0580025573.01 | -0.822362 | 2.467169e-53 | 2.060705e-50 |
| MsG0080048697.01 | MsG0580025573.01 | -0.803865 | 2.842380e-49 | 1.444206e-46 |
| MsG0080048948.01 | MsG0580025573.01 | -0.812716 | 3.686383e-51 | 2.361075e-48 |
| MsG0080048992.01 | MsG0580025573.01 | -0.800659 | 1.298995e-48 | 6.082639e-46 |
| MsG0080049019.01 | MsG0580025573.01 | -0.802387 | 5.746348e-49 | 2.811366e-46 |
| MsG0080049023.01 | MsG0580025573.01 | -0.820606 | 6.277735e-53 | 4.990029e-50 |
| MsG0080049038.01 | MsG0580025573.01 | -0.813408 | 2.598738e-51 | 1.695761e-48 |
| MsG0080049076.01 | MsG0580025573.01 | -0.823114 | 1.649047e-53 | 1.407022e-50 |
| MsG0080049133.01 | MsG0580025573.01 | -0.804663 | 1.939108e-49 | 1.005630e-46 |
| MsG0180000138.01 | MsG0580025573.01 | 0.813793 | 2.137862e-51 | 1.409630e-48 |
| MsG0180000367.01 | MsG0580025573.01 | 0.807178 | 5.739435e-50 | 3.175208e-47 |
| MsG0180000719.01 | MsG0580025573.01 | -0.822690 | 2.069690e-53 | 1.744831e-50 |
| MsG0180001054.01 | MsG0580025573.01 | -0.828871 | 7.065101e-55 | 7.110552e-52 |
| MsG0180001072.01 | MsG0580025573.01 | -0.813366 | 2.654140e-51 | 1.729951e-48 |
| MsG0180001925.01 | MsG0580025573.01 | -0.857824 | 1.254493e-62 | 3.136532e-59 |
| MsG0180002099.01 | MsG0580025573.01 | -0.811335 | 7.371734e-51 | 4.550497e-48 |
| MsG0180002214.01 | MsG0580025573.01 | -0.823830 | 1.121472e-53 | 9.764351e-51 |
| MsG0180002425.01 | MsG0580025573.01 | -0.808338 | 3.252984e-50 | 1.855160e-47 |
| MsG0180002426.01 | MsG0580025573.01 | -0.815461 | 9.137977e-52 | 6.304399e-49 |
| MsG0180002546.01 | MsG0580025573.01 | -0.802060 | 6.710167e-49 | 3.255476e-46 |
| MsG0180004124.01 | MsG0580025573.01 | -0.828811 | 7.303849e-55 | 7.338581e-52 |
| MsG0180004125.01 | MsG0580025573.01 | -0.826062 | 3.333251e-54 | 3.092661e-51 |
| MsG0180004226.01 | MsG0580025573.01 | -0.812623 | 3.861708e-51 | 2.467380e-48 |
| MsG0180004301.01 | MsG0580025573.01 | -0.802162 | 6.393498e-49 | 3.109952e-46 |
| MsG0180004322.01 | MsG0580025573.01 | -0.815825 | 7.578446e-52 | 5.280598e-49 |
| MsG0180004553.01 | MsG0580025573.01 | -0.803178 | 3.945531e-49 | 1.969550e-46 |
| MsG0180004586.01 | MsG0580025573.01 | -0.834388 | 3.085150e-56 | 3.655763e-53 |
| MsG0180004957.01 | MsG0580025573.01 | -0.829549 | 4.837266e-55 | 4.966647e-52 |
| MsG0180005516.01 | MsG0580025573.01 | -0.815173 | 1.058782e-51 | 7.246400e-49 |
| MsG0180005791.01 | MsG0580025573.01 | -0.829108 | 6.189248e-55 | 6.273355e-52 |
| MsG0180005817.01 | MsG0580025573.01 | -0.807109 | 5.935609e-50 | 3.277700e-47 |
| MsG0180005833.01 | MsG0580025573.01 | -0.808498 | 3.006435e-50 | 1.721755e-47 |
| MsG0180006063.01 | MsG0580025573.01 | -0.821444 | 4.023750e-53 | 3.274137e-50 |
| MsG0180006212.01 | MsG0580025573.01 | -0.808853 | 2.524639e-50 | 1.459274e-47 |
| MsG0180006227.01 | MsG0580025573.01 | -0.809335 | 1.991127e-50 | 1.165856e-47 |
| MsG0280006381.01 | MsG0580025573.01 | -0.803061 | 4.172637e-49 | 2.076540e-46 |
| MsG0280010944.01 | MsG0580025573.01 | 0.806793 | 6.922608e-50 | 3.791765e-47 |
| MsG0380012281.01 | MsG0580025573.01 | -0.804423 | 2.176041e-49 | 1.121485e-46 |
| MsG0380013892.01 | MsG0580025573.01 | -0.800015 | 1.757283e-48 | 8.094843e-46 |
| MsG0380013921.01 | MsG0580025573.01 | -0.810340 | 1.209896e-50 | 7.274153e-48 |
| MsG0380013926.01 | MsG0580025573.01 | -0.807551 | 4.784223e-50 | 2.672483e-47 |
| MsG0380013928.01 | MsG0580025573.01 | -0.826701 | 2.347423e-54 | 2.218047e-51 |
| MsG0380013940.01 | MsG0580025573.01 | -0.811774 | 5.916917e-51 | 3.695230e-48 |
| MsG0380013943.01 | MsG0580025573.01 | -0.823672 | 1.221014e-53 | 1.058422e-50 |
| MsG0380013945.01 | MsG0580025573.01 | -0.820736 | 5.860090e-53 | 4.675079e-50 |
| MsG0380013953.01 | MsG0580025573.01 | -0.805631 | 1.216333e-49 | 6.467071e-47 |
| MsG0380013954.01 | MsG0580025573.01 | -0.801767 | 7.707454e-49 | 3.711434e-46 |
| MsG0380013956.01 | MsG0580025573.01 | -0.824780 | 6.705875e-54 | 5.999048e-51 |
| MsG0380013966.01 | MsG0580025573.01 | -0.808453 | 3.074384e-50 | 1.758612e-47 |
| MsG0380013971.01 | MsG0580025573.01 | -0.824584 | 7.457587e-54 | 6.633988e-51 |
| MsG0380013975.01 | MsG0580025573.01 | -0.816647 | 4.964054e-52 | 3.537693e-49 |
| MsG0380013977.01 | MsG0580025573.01 | -0.806002 | 1.016269e-49 | 5.455291e-47 |
| MsG0380013986.01 | MsG0580025573.01 | -0.813637 | 2.314582e-51 | 1.519670e-48 |
| MsG0380014005.01 | MsG0580025573.01 | -0.812375 | 4.376465e-51 | 2.777878e-48 |
| MsG0380014016.01 | MsG0580025573.01 | -0.804021 | 2.638183e-49 | 1.345802e-46 |
| MsG0380014023.01 | MsG0580025573.01 | -0.820477 | 6.719882e-53 | 5.322754e-50 |
| MsG0380014026.01 | MsG0580025573.01 | -0.806165 | 9.392715e-50 | 5.062908e-47 |
| MsG0380014029.01 | MsG0580025573.01 | -0.821240 | 4.485942e-53 | 3.629501e-50 |
| MsG0380014032.01 | MsG0580025573.01 | -0.824101 | 9.684423e-54 | 8.495879e-51 |
| MsG0380014033.01 | MsG0580025573.01 | -0.804200 | 2.420924e-49 | 1.240603e-46 |
| MsG0380014129.01 | MsG0580025573.01 | -0.820486 | 6.689007e-53 | 5.299558e-50 |
| MsG0380014410.01 | MsG0580025573.01 | 0.803086 | 4.121957e-49 | 2.052669e-46 |
| MsG0380014642.01 | MsG0580025573.01 | -0.805321 | 1.412585e-49 | 7.450876e-47 |
| MsG0380014735.01 | MsG0580025573.01 | -0.838093 | 3.528714e-57 | 4.676670e-54 |
| MsG0480019017.01 | MsG0580025573.01 | 0.840791 | 7.029235e-58 | 1.012284e-54 |
| MsG0480019028.01 | MsG0580025573.01 | -0.808108 | 3.641496e-50 | 2.064081e-47 |
| MsG0480019861.01 | MsG0580025573.01 | -0.828015 | 1.136689e-54 | 1.116062e-51 |
| MsG0480020393.01 | MsG0580025573.01 | -0.800559 | 1.361597e-48 | 6.359463e-46 |
| MsG0480020958.01 | MsG0580025573.01 | 0.810511 | 1.111545e-50 | 6.713145e-48 |
| MsG0480021327.01 | MsG0580025573.01 | -0.822978 | 1.773450e-53 | 1.507355e-50 |
| MsG0480021331.01 | MsG0580025573.01 | -0.817740 | 2.818900e-52 | 2.070023e-49 |
| MsG0480021474.01 | MsG0580025573.01 | -0.822305 | 2.543654e-53 | 2.121020e-50 |
| MsG0480021476.01 | MsG0580025573.01 | -0.807721 | 4.400598e-50 | 2.469238e-47 |
| MsG0480021483.01 | MsG0580025573.01 | -0.811181 | 7.961820e-51 | 4.895092e-48 |
| MsG0480021912.01 | MsG0580025573.01 | -0.827775 | 1.298314e-54 | 1.265685e-51 |
| MsG0480022459.01 | MsG0580025573.01 | -0.807988 | 3.861909e-50 | 2.182122e-47 |
| MsG0480022581.01 | MsG0580025573.01 | -0.821955 | 3.066212e-53 | 2.531177e-50 |
| MsG0480022591.01 | MsG0580025573.01 | -0.821240 | 4.485942e-53 | 3.629501e-50 |
| MsG0480023152.01 | MsG0580025573.01 | -0.830887 | 2.279785e-55 | 2.433949e-52 |
| MsG0480023481.01 | MsG0580025573.01 | -0.811289 | 7.541200e-51 | 4.649575e-48 |
| MsG0480023577.01 | MsG0580025573.01 | -0.812270 | 4.613853e-51 | 2.920450e-48 |
| MsG0480023695.01 | MsG0580025573.01 | -0.804832 | 1.787520e-49 | 9.311285e-47 |
| MsG0580024309.01 | MsG0580025573.01 | -0.801986 | 6.951139e-49 | 3.365869e-46 |
| MsG0580024493.01 | MsG0580025573.01 | -0.826579 | 2.510701e-54 | 2.363913e-51 |
| MsG0580025572.01 | MsG0580025573.01 | 0.915850 | 3.604719e-85 | 9.618822e-81 |
| MsG0580025573.01 | MsG0580026362.01 | -0.821383 | 4.156539e-53 | 3.376356e-50 |
| MsG0580025573.01 | MsG0580027677.01 | -0.818122 | 2.311474e-52 | 1.715190e-49 |
| MsG0580025573.01 | MsG0580028159.01 | -0.804337 | 2.267609e-49 | 1.166130e-46 |
| MsG0580025573.01 | MsG0580029623.01 | 0.823490 | 1.346951e-53 | 1.161500e-50 |
| MsG0580025573.01 | MsG0580029792.01 | -0.853383 | 2.477994e-61 | 5.344401e-58 |
| MsG0580025573.01 | MsG0680030878.01 | -0.810460 | 1.139832e-50 | 6.874626e-48 |
| MsG0580025573.01 | MsG0680031062.01 | 0.802201 | 6.278482e-49 | 3.056819e-46 |
| MsG0580025573.01 | MsG0680031813.01 | -0.806027 | 1.003907e-49 | 5.392219e-47 |
| MsG0580025573.01 | MsG0680035870.01 | -0.820627 | 6.206831e-53 | 4.936509e-50 |
| MsG0580025573.01 | MsG0780037458.01 | -0.818762 | 1.654762e-52 | 1.249831e-49 |
| MsG0580025573.01 | MsG0780038047.01 | -0.826650 | 2.414262e-54 | 2.277850e-51 |
| MsG0580025573.01 | MsG0780038197.01 | -0.807231 | 5.592874e-50 | 3.098431e-47 |
| MsG0580025573.01 | MsG0780038573.01 | -0.811057 | 8.470529e-51 | 5.191021e-48 |
| MsG0580025573.01 | MsG0780039662.01 | 0.817783 | 2.756141e-52 | 2.026328e-49 |
| MsG0580025573.01 | MsG0780039836.01 | -0.801191 | 1.011429e-48 | 4.800310e-46 |
| MsG0580025573.01 | MsG0780039838.01 | -0.822678 | 2.083746e-53 | 1.756080e-50 |
| MsG0580025573.01 | MsG0780039839.01 | -0.825159 | 5.457369e-54 | 4.935309e-51 |
| MsG0580025573.01 | MsG0780040121.01 | -0.800537 | 1.376089e-48 | 6.423389e-46 |
| MsG0580025573.01 | MsG0780040838.01 | -0.809670 | 1.687390e-50 | 9.968737e-48 |
| MsG0580025573.01 | MsG0780041121.01 | -0.829595 | 4.713713e-55 | 4.846670e-52 |
| MsG0580025573.01 | MsG0780041531.01 | -0.839702 | 1.352920e-57 | 1.883403e-54 |
| MsG0580025573.01 | MsG0780041637.01 | -0.808164 | 3.543564e-50 | 2.011657e-47 |
| MsG0580025573.01 | MsG0780041720.01 | -0.801618 | 8.270562e-49 | 3.967582e-46 |
| MsG0580025573.01 | MsG0780041783.01 | -0.816934 | 4.281304e-52 | 3.075256e-49 |
| MsG0580025573.01 | MsG0880042291.01 | -0.822703 | 2.055727e-53 | 1.733707e-50 |
| MsG0580025573.01 | MsG0880043069.01 | -0.805287 | 1.435841e-49 | 7.566936e-47 |
| MsG0580025573.01 | MsG0880043261.01 | 0.824651 | 7.191101e-54 | 6.409571e-51 |
| MsG0580025573.01 | MsG0880045188.01 | -0.816981 | 4.178354e-52 | 3.005276e-49 |
| MsG0580025573.01 | MsG0880045321.01 | -0.804375 | 2.226611e-49 | 1.146206e-46 |
| MsG0580025573.01 | MsG0880045369.01 | -0.832490 | 9.175037e-56 | 1.027120e-52 |
| MsG0580025573.01 | MsG0880045385.01 | -0.842829 | 2.036446e-58 | 3.125418e-55 |
| MsG0580025573.01 | MsG0880045413.01 | -0.812503 | 4.102258e-51 | 2.612838e-48 |
| MsG0580025573.01 | MsG0880045414.01 | -0.802505 | 5.434762e-49 | 2.666761e-46 |
| MsG0580025573.01 | MsG0880045630.01 | -0.811047 | 8.510985e-51 | 5.214488e-48 |
| MsG0580025573.01 | MsG0880046167.01 | -0.805119 | 1.556991e-49 | 8.170190e-47 |
| MsG0580025573.01 | MsG0880046174.01 | -0.802199 | 6.282726e-49 | 3.058773e-46 |
| MsG0580025573.01 | MsG0880046469.01 | -0.827314 | 1.675125e-54 | 1.611453e-51 |
| MsG0580025573.01 | MsG0880046630.01 | -0.831967 | 1.236019e-55 | 1.362565e-52 |
| MsG0580025573.01 | MsG0880047318.01 | -0.808365 | 3.210530e-50 | 1.832286e-47 |
| MsG0380015271.01 | MsG0580025573.01 | -0.824981 | 6.012597e-54 | 5.409861e-51 |
| MsG0380015348.01 | MsG0580025573.01 | -0.803468 | 3.437060e-49 | 1.728496e-46 |
| MsG0380015802.01 | MsG0580025573.01 | -0.802231 | 6.189397e-49 | 3.015880e-46 |
| MsG0380016065.01 | MsG0580025573.01 | -0.821017 | 5.047646e-53 | 4.059222e-50 |
| MsG0380016229.01 | MsG0580025573.01 | -0.802533 | 5.363531e-49 | 2.633624e-46 |
| MsG0380016881.01 | MsG0580025573.01 | -0.819796 | 9.623409e-53 | 7.480329e-50 |
| MsG0380016889.01 | MsG0580025573.01 | -0.813404 | 2.603727e-51 | 1.698852e-48 |
| MsG0380016931.01 | MsG0580025573.01 | -0.808827 | 2.557840e-50 | 1.477423e-47 |
| MsG0380016934.01 | MsG0580025573.01 | -0.814467 | 1.518254e-51 | 1.019568e-48 |
| MsG0380016935.01 | MsG0580025573.01 | -0.827574 | 1.450871e-54 | 1.406113e-51 |
| MsG0380017231.01 | MsG0580025573.01 | 0.829086 | 6.267937e-55 | 6.348835e-52 |
| MsG0380017405.01 | MsG0580025573.01 | -0.829994 | 3.769840e-55 | 3.921278e-52 |
| MsG0380017406.01 | MsG0580025573.01 | -0.807887 | 4.058175e-50 | 2.286835e-47 |
| MsG0380017904.01 | MsG0580025573.01 | -0.804065 | 2.582887e-49 | 1.319126e-46 |
| MsG0380017928.01 | MsG0580025573.01 | -0.827073 | 1.913495e-54 | 1.827694e-51 |
| MsG0380017962.01 | MsG0580025573.01 | -0.802974 | 4.349435e-49 | 2.159724e-46 |
| MsG0480018088.01 | MsG0580025573.01 | -0.800051 | 1.727409e-48 | 7.964677e-46 |
| MsG0480018201.01 | MsG0580025573.01 | -0.801317 | 9.531236e-49 | 4.537990e-46 |
| MsG0280007017.01 | MsG0580025573.01 | -0.803014 | 4.266540e-49 | 2.120708e-46 |
| MsG0280007786.01 | MsG0580025573.01 | 0.808741 | 2.667935e-50 | 1.537502e-47 |
| MsG0280008820.01 | MsG0580025573.01 | -0.830535 | 2.781275e-55 | 2.938428e-52 |
| MsG0280009079.01 | MsG0580025573.01 | -0.813702 | 2.239333e-51 | 1.472921e-48 |
| MsG0280009094.01 | MsG0580025573.01 | -0.808695 | 2.728946e-50 | 1.570795e-47 |
| MsG0280009742.01 | MsG0580025573.01 | -0.806229 | 9.102135e-50 | 4.914450e-47 |
| MsG0280009864.01 | MsG0580025573.01 | -0.834389 | 3.083036e-56 | 3.653443e-53 |
| MsG0280009971.01 | MsG0580025573.01 | 0.823594 | 1.273666e-53 | 1.101513e-50 |
| MsG0280010142.01 | MsG0580025573.01 | -0.817446 | 3.283839e-52 | 2.392069e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025573.01.T01 | MTR_5g030740 | 98.438 | 128 | 2 | 0 | 1 | 128 | 2 | 129 | 3.75e-90 | 257 |
| MsG0580025573.01.T01 | MTR_8g067590 | 64.463 | 121 | 37 | 4 | 3 | 122 | 1 | 116 | 1.68e-47 | 149 |
| MsG0580025573.01.T01 | MTR_7g079650 | 85.417 | 48 | 6 | 1 | 81 | 127 | 2 | 49 | 3.24e-22 | 83.2 |
| MsG0580025573.01.T01 | MTR_7g079600 | 77.612 | 67 | 4 | 1 | 1 | 67 | 1 | 56 | 2.48e-20 | 78.6 |
| MsG0580025573.01.T01 | MTR_5g067320 | 36.364 | 110 | 67 | 2 | 16 | 122 | 9 | 118 | 2.59e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025573.01.T01 | AT5G65660 | 55.906 | 127 | 54 | 2 | 2 | 128 | 1 | 125 | 6.48e-43 | 138 |
Find 66 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTATGTAATATGATTTAA+AGG | 0.180489 | 5:+20721831 | MsG0580025573.01.T01:three_prime_UTR |
| ACAATGTATTTGCAGTTCTA+AGG | 0.229478 | 5:+20721720 | MsG0580025573.01.T01:three_prime_UTR |
| AGACTTGGAGGGTGGGGATT+GGG | 0.287949 | 5:-20720974 | None:intergenic |
| TAGACTTGGAGGGTGGGGAT+TGG | 0.378810 | 5:-20720975 | None:intergenic |
| AGTTTAACGGTGTTGATGCC+TGG | 0.390484 | 5:+20721334 | MsG0580025573.01.T01:CDS |
| CTCATCATCTTCAGCTTAAG+TGG | 0.396808 | 5:+20720868 | MsG0580025573.01.T01:CDS |
| CTTGCCCGTGTCAGCATTCG+CGG | 0.412175 | 5:+20721386 | MsG0580025573.01.T01:CDS |
| ACTTGAGGTGGTGAAAGATC+TGG | 0.414803 | 5:-20720793 | None:intergenic |
| AATAGATTATCAAAAGACTA+AGG | 0.435313 | 5:+20721675 | MsG0580025573.01.T01:three_prime_UTR |
| GTATCAATACAAACAATTGA+TGG | 0.443205 | 5:-20721526 | None:intergenic |
| TGGTGGTGGTCACGGTGGTT+TGG | 0.444107 | 5:-20721446 | None:intergenic |
| TCTTCTCATGTTGCTACCAC+TGG | 0.447898 | 5:+20720893 | MsG0580025573.01.T01:CDS |
| GCAAGTTAATTAATTTATGA+TGG | 0.449587 | 5:-20721466 | None:intergenic |
| AGGTTTAGACTTGGAGGGTG+GGG | 0.449727 | 5:-20720980 | None:intergenic |
| TGATAAGCTCTTGATGTTGG+TGG | 0.460503 | 5:-20720748 | None:intergenic |
| GTTTAACGGTGTTGATGCCT+GGG | 0.471943 | 5:+20721335 | MsG0580025573.01.T01:CDS |
| CCGTCCCTCGGTTTCCCACT+AGG | 0.481711 | 5:+20720829 | MsG0580025573.01.T01:CDS |
| CAATGGCCACACAAGACAAC+AGG | 0.486436 | 5:-20721800 | None:intergenic |
| TGAGGTTTAGACTTGGAGGG+TGG | 0.486688 | 5:-20720982 | None:intergenic |
| AAAGAAGGGGCAAAGTTTAA+CGG | 0.488155 | 5:+20721321 | MsG0580025573.01.T01:CDS |
| GAGGTTTAGACTTGGAGGGT+GGG | 0.493079 | 5:-20720981 | None:intergenic |
| CAATGTATTTGCAGTTCTAA+GGG | 0.498460 | 5:+20721721 | MsG0580025573.01.T01:three_prime_UTR |
| TAATTAATTTATGATGGTGG+TGG | 0.504231 | 5:-20721460 | None:intergenic |
| AGTTAATTAATTTATGATGG+TGG | 0.505425 | 5:-20721463 | None:intergenic |
| CAAGGCATGGCTATAAACTT+TGG | 0.507163 | 5:-20721367 | None:intergenic |
| GGTGTTGATGCCTGGGGATG+AGG | 0.513978 | 5:+20721342 | MsG0580025573.01.T01:CDS |
| GGAGTGAGGTTTAGACTTGG+AGG | 0.515823 | 5:-20720986 | None:intergenic |
| ATGCTGACACGGGCAAGGCA+TGG | 0.518272 | 5:-20721380 | None:intergenic |
| TCAATCTCTCTCTCATCTTG+AGG | 0.522920 | 5:+20720936 | MsG0580025573.01.T01:CDS |
| CGTGGAGTGAGGTTTAGACT+TGG | 0.523135 | 5:-20720989 | None:intergenic |
| CTTGAGGTGGTGAAAGATCT+GGG | 0.527529 | 5:-20720792 | None:intergenic |
| GACACATCTCGACCGTCCCT+CGG | 0.527794 | 5:+20720817 | MsG0580025573.01.T01:CDS |
| CGCGAATGCTGACACGGGCA+AGG | 0.530171 | 5:-20721385 | None:intergenic |
| AGCTGAAGATGATGAGCAAG+AGG | 0.534577 | 5:-20720861 | None:intergenic |
| CTTTGATAAGCTCTTGATGT+TGG | 0.534844 | 5:-20720751 | None:intergenic |
| AACTTTGGTACCTCATCCCC+AGG | 0.535358 | 5:-20721352 | None:intergenic |
| AGAGGAGAGCAGTGCCTAGT+GGG | 0.536628 | 5:-20720843 | None:intergenic |
| TCTAGCCGCGAATGCTGACA+CGG | 0.546125 | 5:-20721391 | None:intergenic |
| CTTCTCATGTTGCTACCACT+GGG | 0.549662 | 5:+20720894 | MsG0580025573.01.T01:CDS |
| AAAAGACTAAGGGATTATGC+GGG | 0.556760 | 5:+20721686 | MsG0580025573.01.T01:three_prime_UTR |
| GGTCACGGTGGTTTGGACGA+TGG | 0.561997 | 5:-20721439 | None:intergenic |
| CAAAAGACTAAGGGATTATG+CGG | 0.566577 | 5:+20721685 | MsG0580025573.01.T01:three_prime_UTR |
| TCTCTTCCTGTTGTCTTGTG+TGG | 0.568518 | 5:+20721794 | MsG0580025573.01.T01:three_prime_UTR |
| GGTCGAGATGTGTCTACTTG+AGG | 0.570607 | 5:-20720808 | None:intergenic |
| ATAGATTATCAAAAGACTAA+GGG | 0.570789 | 5:+20721676 | MsG0580025573.01.T01:three_prime_UTR |
| ATTTATGATGGTGGTGGTCA+CGG | 0.583497 | 5:-20721454 | None:intergenic |
| CGGTGGATAGGAAACGGAGG+GGG | 0.590318 | 5:+20721297 | MsG0580025573.01.T01:intron |
| CAACATCAAGAGCTTATCAA+AGG | 0.593842 | 5:+20720752 | MsG0580025573.01.T01:five_prime_UTR |
| TTATCAAAGGTATATGATGA+TGG | 0.597241 | 5:+20720765 | MsG0580025573.01.T01:exon |
| GAGTGAGGTTTAGACTTGGA+GGG | 0.606361 | 5:-20720985 | None:intergenic |
| CTAGCCGCGAATGCTGACAC+GGG | 0.608171 | 5:-20721390 | None:intergenic |
| CAGTGCCTAGTGGGAAACCG+AGG | 0.610817 | 5:-20720834 | None:intergenic |
| CTCGGTGGATAGGAAACGGA+GGG | 0.611696 | 5:+20721295 | MsG0580025573.01.T01:intron |
| CAAGTCTAAACCTCACTCCA+CGG | 0.623681 | 5:+20720990 | MsG0580025573.01.T01:CDS |
| AAGAGGAGAGCAGTGCCTAG+TGG | 0.632457 | 5:-20720844 | None:intergenic |
| AAAGACTAAGGGATTATGCG+GGG | 0.646325 | 5:+20721687 | MsG0580025573.01.T01:three_prime_UTR |
| CGAGATGTGTCTACTTGAGG+TGG | 0.649600 | 5:-20720805 | None:intergenic |
| TACATACATAAATTGCACAA+TGG | 0.654375 | 5:-20721817 | None:intergenic |
| AGTGCCTAGTGGGAAACCGA+GGG | 0.658274 | 5:-20720833 | None:intergenic |
| CAATGTGTTTGTCATAAACG+TGG | 0.660221 | 5:+20721562 | MsG0580025573.01.T01:three_prime_UTR |
| CCTAGTGGGAAACCGAGGGA+CGG | 0.663157 | 5:-20720829 | None:intergenic |
| AAGACTAAGGGATTATGCGG+GGG | 0.675128 | 5:+20721688 | MsG0580025573.01.T01:three_prime_UTR |
| TATGATGGTGGTGGTCACGG+TGG | 0.706241 | 5:-20721451 | None:intergenic |
| TCTCGGTGGATAGGAAACGG+AGG | 0.717298 | 5:+20721294 | MsG0580025573.01.T01:intron |
| TTTAACGGTGTTGATGCCTG+GGG | 0.738355 | 5:+20721336 | MsG0580025573.01.T01:CDS |
| TCGGTGGATAGGAAACGGAG+GGG | 0.773372 | 5:+20721296 | MsG0580025573.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAATACTGCAAATTA+CGG | + | Chr5:20721607-20721626 | MsG0580025573.01.T01:three_prime_UTR | 15.0% |
| !! | ATGTATGTAATATGATTTAA+AGG | + | Chr5:20721831-20721850 | MsG0580025573.01.T01:three_prime_UTR | 15.0% |
| !! | TACTAAAATTATGAAACATT+AGG | - | Chr5:20721045-20721064 | None:intergenic | 15.0% |
| !!! | GTATTTTTTTTGTAGAAATA+CGG | - | Chr5:20721598-20721617 | None:intergenic | 15.0% |
| !! | AATAGATTATCAAAAGACTA+AGG | + | Chr5:20721675-20721694 | MsG0580025573.01.T01:three_prime_UTR | 20.0% |
| !! | AGTTAATTAATTTATGATGG+TGG | - | Chr5:20721466-20721485 | None:intergenic | 20.0% |
| !! | ATAGATTATCAAAAGACTAA+GGG | + | Chr5:20721676-20721695 | MsG0580025573.01.T01:three_prime_UTR | 20.0% |
| !! | GCAAGTTAATTAATTTATGA+TGG | - | Chr5:20721469-20721488 | None:intergenic | 20.0% |
| !!! | CATTAATTTTTCTATACACA+CGG | - | Chr5:20721491-20721510 | None:intergenic | 20.0% |
| !!! | TGATAATCTATTGTTTTTGT+CGG | - | Chr5:20721667-20721686 | None:intergenic | 20.0% |
| ! | AAGTATCATACAACTGTAAA+TGG | - | Chr5:20721185-20721204 | None:intergenic | 25.0% |
| ! | GTATCAATACAAACAATTGA+TGG | - | Chr5:20721529-20721548 | None:intergenic | 25.0% |
| ! | TACATACATAAATTGCACAA+TGG | - | Chr5:20721820-20721839 | None:intergenic | 25.0% |
| !! | TTATCAAAGGTATATGATGA+TGG | + | Chr5:20720765-20720784 | MsG0580025573.01.T01:exon | 25.0% |
| !!! | GAATTTACGATTTTGTTTCT+CGG | + | Chr5:20721277-20721296 | MsG0580025573.01.T01:intron | 25.0% |
| !!! | GATAATCTATTGTTTTTGTC+GGG | - | Chr5:20721666-20721685 | None:intergenic | 25.0% |
| !!! | TAATTAATTTATGATGGTGG+TGG | - | Chr5:20721463-20721482 | None:intergenic | 25.0% |
| ACAATGTATTTGCAGTTCTA+AGG | + | Chr5:20721720-20721739 | MsG0580025573.01.T01:three_prime_UTR | 30.0% | |
| CACGGTAAAAAAATTTACTC+TGG | + | Chr5:20721008-20721027 | MsG0580025573.01.T01:intron | 30.0% | |
| ! | CAATGTATTTGCAGTTCTAA+GGG | + | Chr5:20721721-20721740 | MsG0580025573.01.T01:three_prime_UTR | 30.0% |
| !!! | CAGAGTAAATTTTTTTACCG+TGG | - | Chr5:20721010-20721029 | None:intergenic | 30.0% |
| AAAAGACTAAGGGATTATGC+GGG | + | Chr5:20721686-20721705 | MsG0580025573.01.T01:three_prime_UTR | 35.0% | |
| AAAGAAGGGGCAAAGTTTAA+CGG | + | Chr5:20721321-20721340 | MsG0580025573.01.T01:CDS | 35.0% | |
| CAAAAGACTAAGGGATTATG+CGG | + | Chr5:20721685-20721704 | MsG0580025573.01.T01:three_prime_UTR | 35.0% | |
| CAACATCAAGAGCTTATCAA+AGG | + | Chr5:20720752-20720771 | MsG0580025573.01.T01:five_prime_UTR | 35.0% | |
| CAATGTGTTTGTCATAAACG+TGG | + | Chr5:20721562-20721581 | MsG0580025573.01.T01:three_prime_UTR | 35.0% | |
| CTTTGATAAGCTCTTGATGT+TGG | - | Chr5:20720754-20720773 | None:intergenic | 35.0% | |
| ! | CTTTTCCTCTTACAATGTCA+AGG | + | Chr5:20721202-20721221 | MsG0580025573.01.T01:intron | 35.0% |
| !! | TTTACGATTTTGTTTCTCGG+TGG | + | Chr5:20721280-20721299 | MsG0580025573.01.T01:intron | 35.0% |
| !!! | AATTTTTTTACCGTGGAGTG+AGG | - | Chr5:20721003-20721022 | None:intergenic | 35.0% |
| AAAGACTAAGGGATTATGCG+GGG | + | Chr5:20721687-20721706 | MsG0580025573.01.T01:three_prime_UTR | 40.0% | |
| CAAGGCATGGCTATAAACTT+TGG | - | Chr5:20721370-20721389 | None:intergenic | 40.0% | |
| CTCATCATCTTCAGCTTAAG+TGG | + | Chr5:20720868-20720887 | MsG0580025573.01.T01:CDS | 40.0% | |
| CTTGACCTTGACATTGTAAG+AGG | - | Chr5:20721210-20721229 | None:intergenic | 40.0% | |
| TCAATCTCTCTCTCATCTTG+AGG | + | Chr5:20720936-20720955 | MsG0580025573.01.T01:CDS | 40.0% | |
| TGATAAGCTCTTGATGTTGG+TGG | - | Chr5:20720751-20720770 | None:intergenic | 40.0% | |
| !! | ATTTATGATGGTGGTGGTCA+CGG | - | Chr5:20721457-20721476 | None:intergenic | 40.0% |
| !! | GATTTTGTTTCTCGGTGGAT+AGG | + | Chr5:20721285-20721304 | MsG0580025573.01.T01:intron | 40.0% |
| AAGACTAAGGGATTATGCGG+GGG | + | Chr5:20721688-20721707 | MsG0580025573.01.T01:three_prime_UTR | 45.0% | |
| ACTTGAGGTGGTGAAAGATC+TGG | - | Chr5:20720796-20720815 | None:intergenic | 45.0% | |
| AGCTGAAGATGATGAGCAAG+AGG | - | Chr5:20720864-20720883 | None:intergenic | 45.0% | |
| CAAGTCTAAACCTCACTCCA+CGG | + | Chr5:20720990-20721009 | MsG0580025573.01.T01:CDS | 45.0% | |
| CTTCTCATGTTGCTACCACT+GGG | + | Chr5:20720894-20720913 | MsG0580025573.01.T01:CDS | 45.0% | |
| CTTGAGGTGGTGAAAGATCT+GGG | - | Chr5:20720795-20720814 | None:intergenic | 45.0% | |
| GAGTGAGGTTTAGACTTGGA+GGG | - | Chr5:20720988-20721007 | None:intergenic | 45.0% | |
| GTTTCTCGGTGGATAGGAAA+CGG | + | Chr5:20721291-20721310 | MsG0580025573.01.T01:intron | 45.0% | |
| TCTCTTCCTGTTGTCTTGTG+TGG | + | Chr5:20721794-20721813 | MsG0580025573.01.T01:three_prime_UTR | 45.0% | |
| TCTTCTCATGTTGCTACCAC+TGG | + | Chr5:20720893-20720912 | MsG0580025573.01.T01:CDS | 45.0% | |
| ! | GTGAACGAAACTTTTCCCAG+TGG | - | Chr5:20720912-20720931 | None:intergenic | 45.0% |
| !! | AGTTTAACGGTGTTGATGCC+TGG | + | Chr5:20721334-20721353 | MsG0580025573.01.T01:CDS | 45.0% |
| !! | GTTTAACGGTGTTGATGCCT+GGG | + | Chr5:20721335-20721354 | MsG0580025573.01.T01:CDS | 45.0% |
| !! | TTTAACGGTGTTGATGCCTG+GGG | + | Chr5:20721336-20721355 | MsG0580025573.01.T01:CDS | 45.0% |
| AAACGGAGGGGGAAAAGAAG+GGG | + | Chr5:20721308-20721327 | MsG0580025573.01.T01:CDS | 50.0% | |
| AACTTTGGTACCTCATCCCC+AGG | - | Chr5:20721355-20721374 | None:intergenic | 50.0% | |
| AGGTTTAGACTTGGAGGGTG+GGG | - | Chr5:20720983-20721002 | None:intergenic | 50.0% | |
| CAATGGCCACACAAGACAAC+AGG | - | Chr5:20721803-20721822 | None:intergenic | 50.0% | |
| CGAGATGTGTCTACTTGAGG+TGG | - | Chr5:20720808-20720827 | None:intergenic | 50.0% | |
| CGTGGAGTGAGGTTTAGACT+TGG | - | Chr5:20720992-20721011 | None:intergenic | 50.0% | |
| GAAACGGAGGGGGAAAAGAA+GGG | + | Chr5:20721307-20721326 | MsG0580025573.01.T01:CDS | 50.0% | |
| GAGGTTTAGACTTGGAGGGT+GGG | - | Chr5:20720984-20721003 | None:intergenic | 50.0% | |
| GGAGTGAGGTTTAGACTTGG+AGG | - | Chr5:20720989-20721008 | None:intergenic | 50.0% | |
| GGTCGAGATGTGTCTACTTG+AGG | - | Chr5:20720811-20720830 | None:intergenic | 50.0% | |
| TGAGGTTTAGACTTGGAGGG+TGG | - | Chr5:20720985-20721004 | None:intergenic | 50.0% | |
| AAGAGGAGAGCAGTGCCTAG+TGG | - | Chr5:20720847-20720866 | None:intergenic | 55.0% | |
| AGACTTGGAGGGTGGGGATT+GGG | - | Chr5:20720977-20720996 | None:intergenic | 55.0% | |
| AGAGGAGAGCAGTGCCTAGT+GGG | - | Chr5:20720846-20720865 | None:intergenic | 55.0% | |
| AGTGCCTAGTGGGAAACCGA+GGG | - | Chr5:20720836-20720855 | None:intergenic | 55.0% | |
| CTCGGTGGATAGGAAACGGA+GGG | + | Chr5:20721295-20721314 | MsG0580025573.01.T01:intron | 55.0% | |
| GGAAACGGAGGGGGAAAAGA+AGG | + | Chr5:20721306-20721325 | MsG0580025573.01.T01:intron | 55.0% | |
| TAAGGGATTATGCGGGGGCA+GGG | + | Chr5:20721693-20721712 | MsG0580025573.01.T01:three_prime_UTR | 55.0% | |
| TAGACTTGGAGGGTGGGGAT+TGG | - | Chr5:20720978-20720997 | None:intergenic | 55.0% | |
| TCGGTGGATAGGAAACGGAG+GGG | + | Chr5:20721296-20721315 | MsG0580025573.01.T01:intron | 55.0% | |
| TCTAGCCGCGAATGCTGACA+CGG | - | Chr5:20721394-20721413 | None:intergenic | 55.0% | |
| TCTCGGTGGATAGGAAACGG+AGG | + | Chr5:20721294-20721313 | MsG0580025573.01.T01:intron | 55.0% | |
| !! | TATGATGGTGGTGGTCACGG+TGG | - | Chr5:20721454-20721473 | None:intergenic | 55.0% |
| ATGCTGACACGGGCAAGGCA+TGG | - | Chr5:20721383-20721402 | None:intergenic | 60.0% | |
| CAGTGCCTAGTGGGAAACCG+AGG | - | Chr5:20720837-20720856 | None:intergenic | 60.0% | |
| CCTAGTGGGAAACCGAGGGA+CGG | - | Chr5:20720832-20720851 | None:intergenic | 60.0% | |
| CGGTGGATAGGAAACGGAGG+GGG | + | Chr5:20721297-20721316 | MsG0580025573.01.T01:intron | 60.0% | |
| CTAAGGGATTATGCGGGGGC+AGG | + | Chr5:20721692-20721711 | MsG0580025573.01.T01:three_prime_UTR | 60.0% | |
| CTAGCCGCGAATGCTGACAC+GGG | - | Chr5:20721393-20721412 | None:intergenic | 60.0% | |
| CTTGCCCGTGTCAGCATTCG+CGG | + | Chr5:20721386-20721405 | MsG0580025573.01.T01:CDS | 60.0% | |
| GACACATCTCGACCGTCCCT+CGG | + | Chr5:20720817-20720836 | MsG0580025573.01.T01:CDS | 60.0% | |
| ! | GGTCACGGTGGTTTGGACGA+TGG | - | Chr5:20721442-20721461 | None:intergenic | 60.0% |
| !! | GGTGTTGATGCCTGGGGATG+AGG | + | Chr5:20721342-20721361 | MsG0580025573.01.T01:CDS | 60.0% |
| !! | TGGTGGTGGTCACGGTGGTT+TGG | - | Chr5:20721449-20721468 | None:intergenic | 60.0% |
| CCGTCCCTCGGTTTCCCACT+AGG | + | Chr5:20720829-20720848 | MsG0580025573.01.T01:CDS | 65.0% | |
| CGCGAATGCTGACACGGGCA+AGG | - | Chr5:20721388-20721407 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 20720742 | 20721878 | 20720742 | ID=MsG0580025573.01;Name=MsG0580025573.01 |
| Chr5 | mRNA | 20720742 | 20721878 | 20720742 | ID=MsG0580025573.01.T01;Parent=MsG0580025573.01;Name=MsG0580025573.01.T01;_AED=0.13;_eAED=0.13;_QI=36|1|1|1|1|1|2|419|128 |
| Chr5 | exon | 20721307 | 20721878 | 20721307 | ID=MsG0580025573.01.T01:exon:12660;Parent=MsG0580025573.01.T01 |
| Chr5 | exon | 20720742 | 20721011 | 20720742 | ID=MsG0580025573.01.T01:exon:12659;Parent=MsG0580025573.01.T01 |
| Chr5 | five_prime_UTR | 20720742 | 20720777 | 20720742 | ID=MsG0580025573.01.T01:five_prime_utr;Parent=MsG0580025573.01.T01 |
| Chr5 | CDS | 20720778 | 20721011 | 20720778 | ID=MsG0580025573.01.T01:cds;Parent=MsG0580025573.01.T01 |
| Chr5 | CDS | 20721307 | 20721459 | 20721307 | ID=MsG0580025573.01.T01:cds;Parent=MsG0580025573.01.T01 |
| Chr5 | three_prime_UTR | 20721460 | 20721878 | 20721460 | ID=MsG0580025573.01.T01:three_prime_utr;Parent=MsG0580025573.01.T01 |
| Gene Sequence |
| Protein sequence |
