AlfalfaGEDB | MsG0180004957.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004957.01

KEGG: K24242
GO: GO:0000287 GO:0005737 GO:0008253
PFAM: PF05822

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004957.01.T01	AES61917.1	90.588	255	12	2	1	255	1	243	7.78E-162	460

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004957.01.T01	Q9D020	38.866	247	126	5	13	255	57	282	1.45E-45	158

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004957.01.T01	G7I312	90.588	255	12	2	1	255	1	243	3.72e-162	460

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047831.01	MsG0180004957.01	-0.852606	4.135586e-61	8.688230e-58
MsG0080047910.01	MsG0180004957.01	0.806205	9.211835e-50	4.970537e-47
MsG0080047967.01	MsG0180004957.01	0.856635	2.816209e-62	6.764883e-59
MsG0080048036.01	MsG0180004957.01	-0.849558	2.993817e-60	5.697103e-57
MsG0080048059.01	MsG0180004957.01	0.887054	1.997307e-72	1.462558e-68
MsG0080048256.01	MsG0180004957.01	-0.809691	1.669609e-50	9.869127e-48
MsG0080048301.01	MsG0180004957.01	-0.816601	5.083593e-52	3.618218e-49
MsG0080048313.01	MsG0180004957.01	0.837075	6.435961e-57	8.267267e-54
MsG0080048347.01	MsG0180004957.01	-0.825538	4.437687e-54	4.056355e-51
MsG0080048352.01	MsG0180004957.01	-0.822771	1.981960e-53	1.674768e-50

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004957.01.T01	MTR_1g090380	90.588	255	12	2	1	255	1	243	9.42e-166	460

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004957.01.T01	AT2G38680	65.504	258	79	5	1	255	41	291	1.60e-111	325

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Find 90 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTACATGCAATAAGGG+GGG	0.667560	1:+84952886	None:intergenic
AAAGGAAACTCATGGTAGAG+TGG	0.614036	1:-84951965	MsG0180004957.01.T01:intron
AAAGTGACGTGGGAGAAAGT+TGG	0.564371	1:+84952983	None:intergenic
AAATGAGCATGCTCTTGATA+TGG	0.389669	1:-84950748	MsG0180004957.01.T01:CDS
AACAGGATGGTATTTAACAA+TGG	0.634907	1:-84951227	MsG0180004957.01.T01:CDS
AACCCAAAACTTTGTTAATG+TGG	0.470399	1:+84952212	None:intergenic
AATCGCCAGCCATATCCAGG+AGG	0.724907	1:+84949820	None:intergenic
ACGCATGCTCTTCTTATTGA+AGG	0.454576	1:-84951714	MsG0180004957.01.T01:CDS
ACTTGTTAGCATTCTTCATA+AGG	0.393716	1:+84949874	None:intergenic
ACTTTGATAACTTCCCACAT+GGG	0.653578	1:+84950006	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTACATGCAATAAGGG+GGG	+	Chr1:84950009-84950028	None:intergenic	30.0%
AAAGGTAAAAAAGAGAAGAG+GGG	+	Chr1:84949980-84949999	None:intergenic	30.0%
AACAGGATGGTATTTAACAA+TGG	-	Chr1:84951665-84951684	MsG0180004957.01.T01:CDS	30.0%
AACCCAAAACTTTGTTAATG+TGG	+	Chr1:84950683-84950702	None:intergenic	30.0%
AGCTTCATTGTAAACATACA+TGG	+	Chr1:84953135-84953154	None:intergenic	30.0%
ATATGAACATTACCATCCAT+TGG	-	Chr1:84950874-84950893	MsG0180004957.01.T01:intron	30.0%
ATTAATGTTTGGAAGGAACA+AGG	+	Chr1:84952711-84952730	None:intergenic	30.0%
ATTAGCAGTTATGTTATGCT+AGG	-	Chr1:84953092-84953111	MsG0180004957.01.T01:five_prime_UTR	30.0%
ATTGTTAAATACCATCCTGT+TGG	+	Chr1:84951666-84951685	None:intergenic	30.0%
CCCAGAAAAAAAATTAAGTC+AGG	+	Chr1:84952420-84952439	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	84949742	84953172	84949742	ID=MsG0180004957.01;Name=MsG0180004957.01
Chr1	mRNA	84949742	84953172	84949742	ID=MsG0180004957.01.T01;Parent=MsG0180004957.01;Name=MsG0180004957.01.T01;_AED=0.49;_eAED=0.49;_QI=339\|0.87\|0.77\|1\|0.75\|0.66\|9\|388\|255
Chr1	exon	84949742	84950037	84949742	ID=MsG0180004957.01.T01:exon:28045;Parent=MsG0180004957.01.T01
Chr1	exon	84950245	84950305	84950245	ID=MsG0180004957.01.T01:exon:28044;Parent=MsG0180004957.01.T01
Chr1	exon	84950589	84950795	84950589	ID=MsG0180004957.01.T01:exon:28043;Parent=MsG0180004957.01.T01
Chr1	exon	84951207	84951300	84951207	ID=MsG0180004957.01.T01:exon:28042;Parent=MsG0180004957.01.T01
Chr1	exon	84951419	84951522	84951419	ID=MsG0180004957.01.T01:exon:28041;Parent=MsG0180004957.01.T01
Chr1	exon	84951652	84951746	84951652	ID=MsG0180004957.01.T01:exon:28040;Parent=MsG0180004957.01.T01
Chr1	exon	84951966	84952098	84951966	ID=MsG0180004957.01.T01:exon:28039;Parent=MsG0180004957.01.T01
Chr1	exon	84952193	84952253	84952193	ID=MsG0180004957.01.T01:exon:28038;Parent=MsG0180004957.01.T01
Chr1	exon	84952729	84953172	84952729	ID=MsG0180004957.01.T01:exon:28037;Parent=MsG0180004957.01.T01
Chr1	five_prime_UTR	84952834	84953172	84952834	ID=MsG0180004957.01.T01:five_prime_utr;Parent=MsG0180004957.01.T01
Chr1	CDS	84952729	84952833	84952729	ID=MsG0180004957.01.T01:cds;Parent=MsG0180004957.01.T01
Chr1	CDS	84952193	84952253	84952193	ID=MsG0180004957.01.T01:cds;Parent=MsG0180004957.01.T01
Chr1	CDS	84951966	84952098	84951966	ID=MsG0180004957.01.T01:cds;Parent=MsG0180004957.01.T01
Chr1	CDS	84951652	84951746	84951652	ID=MsG0180004957.01.T01:cds;Parent=MsG0180004957.01.T01
Chr1	CDS	84951419	84951522	84951419	ID=MsG0180004957.01.T01:cds;Parent=MsG0180004957.01.T01
Chr1	CDS	84951207	84951300	84951207	ID=MsG0180004957.01.T01:cds;Parent=MsG0180004957.01.T01
Chr1	CDS	84950620	84950795	84950620	ID=MsG0180004957.01.T01:cds;Parent=MsG0180004957.01.T01
Chr1	three_prime_UTR	84950589	84950619	84950589	ID=MsG0180004957.01.T01:three_prime_utr;Parent=MsG0180004957.01.T01
Chr1	three_prime_UTR	84950245	84950305	84950245	ID=MsG0180004957.01.T01:three_prime_utr;Parent=MsG0180004957.01.T01
Chr1	three_prime_UTR	84949742	84950037	84949742	ID=MsG0180004957.01.T01:three_prime_utr;Parent=MsG0180004957.01.T01

Gene Sequence

>MsG0180004957.01.T01

ATGGAGGATATCAAATTGGGTTCAGAATTGACGCTGGTGGGTGATCCTGTTTTGCTCCACAACAAACTTGCTGCAATTCGTTCCGCTGGTCCTCAAAAGCTTCAGGTGATCGCTGATTTTGATGCCACATTAACAAAGTTTTGGGTTAATGGAACTCGCGGCCAAAGCAGTCATGGCCTTTTGCAGCAGGATAATCCAGAATATGATGCCAAAAGGCAGCAGTTATATGAACATTACCATCCATTGGAATTTTCTCCTACCCTTGGGCTGGAAGAGAAAAGGAAACTCATGGTAGAGTGGTGGGGGAAAACGCATGCTCTTCTTATTGAAGGAGGACTTACATATGAATCAATAAAACAATCAGTTGCTAATGCTAACATAGCTTTTAGGGAAGATTCGATTGCTGACATTGGATACCATTTTCCATTTTGTTATTTTTCAGGAAGAGACATTCCTGTATTAATATTCTCTGCAGGGCTTGCTGATATCATTGAAGAGGTTCTAAGGCAGAAGCTTCGCAGATCCTTCAAAAATGTGAAGATAGTATCCAACAGGATGGTATTTAACAATGGCCAACTTGTATCTTTTAAAGGAAAATTGATTCACAGCTTAAATAAAAATGAGCATGCTCTTGATATGGCTGCGCCTGTTCACGAACGATTCGGTGATATTGATGGTCCAACCGATGACAATGACTTGCTCAAGAAGAGAACCAATGTTCTCCTTCTTGGTGATCACACTGGAGACTTGGGAATGTCTGATGGTTAA

Protein sequence

>MsG0180004957.01.T01

MEDIKLGSELTLVGDPVLLHNKLAAIRSAGPQKLQVIADFDATLTKFWVNGTRGQSSHGLLQQDNPEYDAKRQQLYEHYHPLEFSPTLGLEEKRKLMVEWWGKTHALLIEGGLTYESIKQSVANANIAFREDSIADIGYHFPFCYFSGRDIPVLIFSAGLADIIEEVLRQKLRRSFKNVKIVSNRMVFNNGQLVSFKGKLIHSLNKNEHALDMAAPVHERFGDIDGPTDDNDLLKKRTNVLLLGDHTGDLGMSDG*