AlfalfaGEDB | MsG0180001689.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001689.01

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001689.01.T01	XP_013467007.1	82.231	242	40	2	1	239	120	361	2.58E-134	393

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001689.01.T01	A0A072VGV2	82.231	242	40	2	1	239	120	361	1.23e-134	393

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0480019459.01	MsG0180001689.01	PPI
MsG0580025051.01	MsG0180001689.01	PPI
MsG0480022014.01	MsG0180001689.01	PPI
MsG0580028665.01	MsG0180001689.01	PPI
MsG0580028665.01	MsG0180001689.01	PPI
MsG0680032353.01	MsG0180001689.01	PPI
MsG0780037596.01	MsG0180001689.01	PPI
MsG0680032353.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0480019471.01	PPI
MsG0880044282.01	MsG0180001689.01	PPI
MsG0780036062.01	MsG0180001689.01	PPI
MsG0380017414.01	MsG0180001689.01	PPI
MsG0780037571.01	MsG0180001689.01	PPI
MsG0480022007.01	MsG0180001689.01	PPI
MsG0680032353.01	MsG0180001689.01	PPI
MsG0780037522.01	MsG0180001689.01	PPI
MsG0480022013.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0680032369.01	PPI
MsG0180001689.01	MsG0780037534.01	PPI
MsG0780037596.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0480022015.01	PPI
MsG0180001689.01	MsG0480022024.01	PPI
MsG0180001689.01	MsG0680032353.01	PPI
MsG0580028757.01	MsG0180001689.01	PPI
MsG0780038471.01	MsG0180001689.01	PPI
MsG0680031415.01	MsG0180001689.01	PPI
MsG0780037534.01	MsG0180001689.01	PPI
MsG0780037066.01	MsG0180001689.01	PPI
MsG0480022009.01	MsG0180001689.01	PPI
MsG0280010541.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0680032353.01	PPI
MsG0580028652.01	MsG0180001689.01	PPI
MsG0380016794.01	MsG0180001689.01	PPI
MsG0480022109.01	MsG0180001689.01	PPI
MsG0380017846.01	MsG0180001689.01	PPI
MsG0380016761.01	MsG0180001689.01	PPI
MsG0580028757.01	MsG0180001689.01	PPI
MsG0880042381.01	MsG0180001689.01	PPI
MsG0780036908.01	MsG0180001689.01	PPI
MsG0780038469.01	MsG0180001689.01	PPI
MsG0580030111.01	MsG0180001689.01	PPI
MsG0780038156.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0280006373.01	PPI
MsG0280010925.01	MsG0180001689.01	PPI
MsG0680032369.01	MsG0180001689.01	PPI
MsG0880044888.01	MsG0180001689.01	PPI
MsG0780037524.01	MsG0180001689.01	PPI
MsG0780038159.01	MsG0180001689.01	PPI
MsG0580028665.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0480020252.01	PPI
MsG0180001689.01	MsG0580028665.01	PPI
MsG0880042352.01	MsG0180001689.01	PPI
MsG0680032369.01	MsG0180001689.01	PPI
MsG0480021723.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0780037592.01	PPI
MsG0180001689.01	MsG0680032369.01	PPI
MsG0680033026.01	MsG0180001689.01	PPI
MsG0380013669.01	MsG0180001689.01	PPI
MsG0280006326.01	MsG0180001689.01	PPI
MsG0680032369.01	MsG0180001689.01	PPI
MsG0780038468.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0580028665.01	PPI
MsG0180001689.01	MsG0780037591.01	PPI
MsG0480019175.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0280006396.01	PPI
MsG0380017416.01	MsG0180001689.01	PPI
MsG0680031417.01	MsG0180001689.01	PPI
MsG0280006398.01	MsG0180001689.01	PPI
MsG0780036907.01	MsG0180001689.01	PPI
MsG0680032766.01	MsG0180001689.01	PPI
MsG0680031413.01	MsG0180001689.01	PPI
MsG0380017847.01	MsG0180001689.01	PPI
MsG0680034526.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0880045337.01	PPI
MsG0680031419.01	MsG0180001689.01	PPI
MsG0680032353.01	MsG0180001689.01	PPI
MsG0680032353.01	MsG0180001689.01	PPI
MsG0780037597.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0580028757.01	PPI
MsG0180001689.01	MsG0180005047.01	PPI
MsG0580028665.01	MsG0180001689.01	PPI
MsG0480021507.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0380013675.01	PPI
MsG0680032369.01	MsG0180001689.01	PPI
MsG0580028665.01	MsG0180001689.01	PPI
MsG0780040052.01	MsG0180001689.01	PPI
MsG0880042360.01	MsG0180001689.01	PPI
MsG0780037562.01	MsG0180001689.01	PPI
MsG0280006373.01	MsG0180001689.01	PPI
MsG0580028638.01	MsG0180001689.01	PPI
MsG0280010911.01	MsG0180001689.01	PPI
MsG0280006373.01	MsG0180001689.01	PPI
MsG0880047230.01	MsG0180001689.01	PPI
MsG0480019463.01	MsG0180001689.01	PPI
MsG0480022017.01	MsG0180001689.01	PPI
MsG0880047562.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0780037555.01	PPI
MsG0380012429.01	MsG0180001689.01	PPI
MsG0480021722.01	MsG0180001689.01	PPI
MsG0780038466.01	MsG0180001689.01	PPI
MsG0780038157.01	MsG0180001689.01	PPI
MsG0780038463.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0780038466.01	PPI
MsG0780037557.01	MsG0180001689.01	PPI
MsG0680032369.01	MsG0180001689.01	PPI
MsG0180001689.01	MsG0280006385.01	PPI
MsG0180001689.01	MsG0580029293.01	PPI
MsG0780038462.01	MsG0180001689.01	PPI
MsG0380017413.01	MsG0180001689.01	PPI
MsG0080048094.01	MsG0180001689.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001689.01.T01	MTR_1g041245	82.231	242	40	2	1	239	120	361	3.12e-138	393
MsG0180001689.01.T01	MTR_7g034115	57.384	237	97	4	1	235	120	354	2.01e-81	248
MsG0180001689.01.T01	MTR_7g032370	52.282	241	101	5	1	239	99	327	1.21e-67	212
MsG0180001689.01.T01	MTR_7g032380	51.695	236	84	4	1	235	121	327	1.38e-65	207
MsG0180001689.01.T01	MTR_7g034120	47.321	224	109	6	14	234	65	282	2.30e-56	182
MsG0180001689.01.T01	MTR_7g033990	51.111	225	97	7	14	235	140	354	5.07e-55	182
MsG0180001689.01.T01	MTR_7g033915	50.761	197	90	5	41	235	145	336	1.37e-51	171
MsG0180001689.01.T01	MTR_7g115270	44.715	246	105	10	8	232	134	369	5.43e-51	171
MsG0180001689.01.T01	MTR_7g056433	44.958	238	109	10	10	235	132	359	2.73e-49	166
MsG0180001689.01.T01	MTR_7g088260	39.749	239	133	6	2	234	199	432	8.53e-45	156
MsG0180001689.01.T01	MTR_5g012840	42.478	226	89	5	14	239	110	294	9.92e-45	152
MsG0180001689.01.T01	MTR_3g108150	38.017	242	141	4	1	235	118	357	4.99e-41	144
MsG0180001689.01.T01	MTR_3g109982	40.343	233	125	7	10	235	220	445	8.92e-41	148
MsG0180001689.01.T01	MTR_7g098510	38.559	236	134	6	6	235	131	361	1.38e-40	143
MsG0180001689.01.T01	MTR_7g098570	38.298	235	134	6	6	234	133	362	2.49e-39	142
MsG0180001689.01.T01	MTR_5g008920	37.288	236	141	4	6	238	125	356	2.67e-37	134
MsG0180001689.01.T01	MTR_5g013500	39.815	216	110	8	36	235	124	335	4.23e-37	134
MsG0180001689.01.T01	MTR_3g108560	42.489	233	115	9	14	235	135	359	8.44e-37	133
MsG0180001689.01.T01	MTR_0492s0020	38.889	234	125	9	9	235	198	420	9.44e-37	134
MsG0180001689.01.T01	MTR_0543s0010	38.889	234	125	9	9	235	198	420	1.29e-36	134
MsG0180001689.01.T01	MTR_3g087260	41.202	233	124	7	10	235	201	427	2.06e-36	134
MsG0180001689.01.T01	MTR_3g087260	31.288	163	94	5	10	168	114	262	7.17e-11	62.0
MsG0180001689.01.T01	MTR_3g036250	36.287	237	126	6	9	237	123	342	3.85e-36	131
MsG0180001689.01.T01	MTR_5g008950	34.454	238	139	7	9	238	112	340	5.99e-36	131
MsG0180001689.01.T01	MTR_3g107100	37.083	240	133	7	5	234	127	358	3.60e-35	129
MsG0180001689.01.T01	MTR_7g007080	38.034	234	125	7	10	232	129	353	5.87e-35	128
MsG0180001689.01.T01	MTR_7g075820	34.043	235	135	7	9	232	141	366	5.27e-34	126
MsG0180001689.01.T01	MTR_5g083890	34.426	244	130	8	9	232	141	374	1.04e-33	127
MsG0180001689.01.T01	MTR_7g098480	34.764	233	143	5	6	234	131	358	2.67e-33	125
MsG0180001689.01.T01	MTR_7g056020	40.000	230	121	9	14	235	124	344	4.55e-33	126
MsG0180001689.01.T01	MTR_3g082890	36.905	252	125	10	8	232	210	454	6.28e-33	124
MsG0180001689.01.T01	MTR_3g099830	35.246	244	124	9	9	232	117	346	2.59e-31	118
MsG0180001689.01.T01	MTR_8g085430	37.759	241	75	8	1	235	128	299	2.76e-31	117
MsG0180001689.01.T01	MTR_2g007450	32.819	259	132	10	5	232	145	392	1.37e-30	117
MsG0180001689.01.T01	MTR_5g073420	34.914	232	131	8	5	232	129	344	1.45e-30	117
MsG0180001689.01.T01	MTR_7g007340	35.745	235	128	8	9	232	107	329	3.08e-30	115
MsG0180001689.01.T01	MTR_2g007400	33.884	242	133	9	9	232	143	375	3.41e-30	116
MsG0180001689.01.T01	MTR_7g056287	39.394	231	119	11	14	237	129	345	6.44e-30	115
MsG0180001689.01.T01	MTR_1g066830	33.750	240	127	9	14	235	131	356	6.49e-30	115
MsG0180001689.01.T01	MTR_1g108820	36.800	250	123	12	7	235	120	355	6.90e-30	115
MsG0180001689.01.T01	MTR_5g073370	36.596	235	130	8	9	235	127	350	2.73e-29	113
MsG0180001689.01.T01	MTR_5g007410	32.766	235	136	7	14	233	149	376	6.33e-29	113
MsG0180001689.01.T01	MTR_5g058100	34.746	236	130	10	12	235	130	353	6.47e-29	112
MsG0180001689.01.T01	MTR_2g008320	33.613	238	134	7	9	232	172	399	1.21e-28	112
MsG0180001689.01.T01	MTR_3g107120	34.348	230	139	5	9	232	141	364	1.34e-28	112
MsG0180001689.01.T01	MTR_7g083740	35.577	208	108	8	38	232	143	337	1.48e-28	111
MsG0180001689.01.T01	MTR_1g107385	33.058	242	133	8	9	232	165	395	1.64e-28	112
MsG0180001689.01.T01	MTR_7g056277	37.931	232	116	10	14	235	385	598	2.21e-28	113
MsG0180001689.01.T01	MTR_5g045170	35.391	243	132	10	7	234	128	360	2.26e-28	111
MsG0180001689.01.T01	MTR_3g107160	31.250	240	149	7	3	234	127	358	3.06e-28	110
MsG0180001689.01.T01	MTR_3g023630	34.468	235	147	5	1	234	119	347	4.39e-28	110
MsG0180001689.01.T01	MTR_2g008310	33.473	239	131	8	9	232	118	343	6.89e-28	109
MsG0180001689.01.T01	MTR_3g040700	34.361	227	119	7	9	225	161	367	9.19e-28	110
MsG0180001689.01.T01	MTR_1g108800	36.441	236	123	9	14	237	94	314	1.84e-27	107
MsG0180001689.01.T01	MTR_2g007360	32.794	247	134	10	9	232	107	344	3.37e-27	107
MsG0180001689.01.T01	MTR_7g026500	35.246	244	124	10	9	235	141	367	3.65e-27	107
MsG0180001689.01.T01	MTR_2g020180	35.323	201	82	6	62	235	9	188	4.19e-27	103
MsG0180001689.01.T01	MTR_7g034110	35.616	219	98	5	14	232	135	310	6.70e-27	106
MsG0180001689.01.T01	MTR_6g040270	32.751	229	136	7	14	235	131	348	7.41e-27	107
MsG0180001689.01.T01	MTR_2g005990	33.610	241	131	8	10	232	138	367	7.76e-27	107
MsG0180001689.01.T01	MTR_3g082890	31.724	290	127	11	9	234	131	413	8.98e-27	107
MsG0180001689.01.T01	MTR_1g085120	31.624	234	140	7	14	235	216	441	1.74e-26	107
MsG0180001689.01.T01	MTR_1g085120	31.624	234	140	7	14	235	291	516	2.74e-26	106
MsG0180001689.01.T01	MTR_7g114710	32.377	244	133	7	1	238	123	340	3.14e-26	104
MsG0180001689.01.T01	MTR_7g081290	50.000	112	51	3	129	236	122	232	6.23e-26	102
MsG0180001689.01.T01	MTR_6g045333	34.454	238	134	10	10	232	183	413	2.36e-25	103
MsG0180001689.01.T01	MTR_7g056430	33.745	243	119	7	7	235	121	335	2.62e-25	104
MsG0180001689.01.T01	MTR_7g056420	33.745	243	119	7	7	235	121	335	3.09e-25	104
MsG0180001689.01.T01	MTR_1089s0010	33.921	227	134	7	14	235	131	346	3.18e-25	102
MsG0180001689.01.T01	MTR_5g054920	35.938	192	94	8	59	235	3	180	6.33e-25	97.8
MsG0180001689.01.T01	MTR_5g058000	33.624	229	133	8	14	236	130	345	5.54e-24	99.0
MsG0180001689.01.T01	MTR_2g036470	33.600	250	126	9	10	236	135	367	7.51e-24	99.0
MsG0180001689.01.T01	MTR_1g053025	30.279	251	131	11	9	232	128	361	8.08e-24	98.6
MsG0180001689.01.T01	MTR_6g022500	33.040	227	136	8	14	232	225	443	1.01e-23	99.4
MsG0180001689.01.T01	MTR_4g039440	33.613	238	125	11	14	232	153	376	1.17e-23	98.6
MsG0180001689.01.T01	MTR_4g080610	35.071	211	114	8	36	232	124	325	1.20e-23	97.8
MsG0180001689.01.T01	MTR_5g039470	31.452	248	128	9	6	228	97	327	1.61e-23	97.1
MsG0180001689.01.T01	MTR_3g105060	30.705	241	148	6	1	237	124	349	2.61e-23	97.1
MsG0180001689.01.T01	MTR_3g115430	46.602	103	50	2	139	238	1	101	4.76e-23	90.9
MsG0180001689.01.T01	MTR_6g029350	33.898	177	105	5	61	232	1	170	7.30e-23	93.6
MsG0180001689.01.T01	MTR_3g110010	37.931	174	95	6	10	176	220	387	1.21e-22	96.7
MsG0180001689.01.T01	MTR_7g022105	32.479	234	142	7	10	232	165	393	1.45e-22	95.5
MsG0180001689.01.T01	MTR_6g022520	38.514	148	82	5	91	235	142	283	4.73e-22	92.8
MsG0180001689.01.T01	MTR_6g022360	30.000	230	148	8	14	235	154	378	9.25e-22	93.2
MsG0180001689.01.T01	MTR_3g071850	29.918	244	143	8	10	235	146	379	4.63e-21	92.0
MsG0180001689.01.T01	MTR_5g039450	31.858	226	111	7	36	236	132	339	7.89e-21	90.1
MsG0180001689.01.T01	MTR_7g055943	31.760	233	137	8	14	232	164	388	9.09e-21	90.5
MsG0180001689.01.T01	MTR_3g026920	29.762	252	124	12	5	232	159	381	1.02e-20	90.1
MsG0180001689.01.T01	MTR_7g060470	30.242	248	133	10	11	232	126	359	1.36e-20	89.7
MsG0180001689.01.T01	MTR_7g021990	31.780	236	141	8	10	232	164	392	1.85e-20	89.7
MsG0180001689.01.T01	MTR_0009s0080	33.476	233	134	10	14	232	142	367	2.35e-20	89.4
MsG0180001689.01.T01	MTR_1377s0010	36.719	128	76	2	106	233	6	128	2.88e-20	84.0
MsG0180001689.01.T01	MTR_7g021950	31.780	236	141	8	10	232	164	392	2.91e-20	89.4
MsG0180001689.01.T01	MTR_7g056280	34.239	184	104	5	62	232	367	546	3.05e-20	89.4
MsG0180001689.01.T01	MTR_3g467400	32.917	240	119	10	9	238	133	340	3.45e-20	88.2
MsG0180001689.01.T01	MTR_5g031970	31.250	240	134	11	3	236	113	327	9.28e-20	87.0
MsG0180001689.01.T01	MTR_2g007090	34.286	210	97	9	35	232	86	266	2.15e-19	85.1
MsG0180001689.01.T01	MTR_2g007550	38.983	118	61	3	119	234	205	313	8.50e-19	84.3
MsG0180001689.01.T01	MTR_3g107940	45.370	108	42	3	133	231	89	188	1.11e-18	84.0
MsG0180001689.01.T01	MTR_7g007370	32.766	235	135	7	9	231	93	316	2.37e-18	82.8
MsG0180001689.01.T01	MTR_7g075800	27.237	257	137	9	9	224	140	387	2.39e-18	84.0
MsG0180001689.01.T01	MTR_4g098590	33.193	238	136	9	9	232	126	354	2.66e-18	83.2
MsG0180001689.01.T01	MTR_3g095630	34.194	155	92	5	80	232	5	151	2.96e-18	80.1
MsG0180001689.01.T01	MTR_6g011460	32.218	239	143	9	6	236	162	389	1.36e-17	81.3
MsG0180001689.01.T01	MTR_5g073340	33.824	204	116	6	10	200	102	299	1.81e-17	80.1
MsG0180001689.01.T01	MTR_3g108560	40.351	171	86	7	14	174	135	299	1.83e-17	80.1
MsG0180001689.01.T01	MTR_5g033200	32.877	146	90	3	90	235	12	149	7.64e-17	76.3
MsG0180001689.01.T01	MTR_3g113785	42.424	99	44	2	133	225	4	95	1.03e-16	73.6
MsG0180001689.01.T01	MTR_5g045510	30.213	235	120	10	14	232	133	339	1.25e-15	75.5
MsG0180001689.01.T01	MTR_1g108820	33.871	186	96	9	7	172	120	298	3.35e-15	74.3
MsG0180001689.01.T01	MTR_3g071790	28.261	184	108	6	59	226	27	202	3.39e-15	72.0
MsG0180001689.01.T01	MTR_2g007500	29.646	226	125	9	9	211	120	334	7.09e-15	73.2
MsG0180001689.01.T01	MTR_3g094980	41.667	84	46	1	152	232	34	117	7.11e-15	70.5
MsG0180001689.01.T01	MTR_3g106960	34.228	149	90	5	91	235	30	174	6.19e-14	69.3
MsG0180001689.01.T01	MTR_1g085120	30.409	171	102	6	14	172	303	468	1.67e-13	69.7
MsG0180001689.01.T01	MTR_3g113770	27.982	218	85	6	14	222	180	334	3.81e-13	68.2
MsG0180001689.01.T01	MTR_6g044572	28.514	249	134	11	1	233	133	353	3.95e-13	68.6
MsG0180001689.01.T01	MTR_1g059860	28.682	258	126	10	14	225	66	311	6.83e-13	67.4
MsG0180001689.01.T01	MTR_6g044560	28.916	249	133	11	1	233	309	529	8.78e-13	67.8
MsG0180001689.01.T01	MTR_4g096730	49.206	63	32	0	175	237	2	64	2.50e-12	61.6
MsG0180001689.01.T01	MTR_1g043170	49.091	55	28	0	180	234	6	60	4.23e-12	60.8
MsG0180001689.01.T01	MTR_6g013160	29.954	217	108	10	17	194	167	378	4.32e-12	65.5
MsG0180001689.01.T01	MTR_2g007610	36.066	122	66	3	114	232	11	123	6.52e-12	62.4
MsG0180001689.01.T01	MTR_5g039490	30.736	231	123	9	14	236	146	347	7.62e-12	64.7
MsG0180001689.01.T01	MTR_4g056020	29.091	110	72	2	121	227	4	110	9.17e-12	62.0
MsG0180001689.01.T01	MTR_7g056417	33.974	156	70	8	10	143	7	151	1.33e-11	61.2
MsG0180001689.01.T01	MTR_5g076150	29.858	211	112	9	14	197	26	227	6.02e-11	60.8
MsG0180001689.01.T01	MTR_4g056020	26.897	145	91	4	88	227	272	406	8.26e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 59 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTTGCAATTCCTCTAAAA+TGG	0.149160	1:+25346046	None:intergenic
CTTAGAATGTGCTCGCTTAT+AGG	0.200434	1:-25345490	MsG0180001689.01.T01:CDS
CTTGCTCAAAACTCTTCATT+TGG	0.299209	1:-25346129	MsG0180001689.01.T01:CDS
GTGAAATTGGCGATAACAAT+TGG	0.311289	1:-25345552	MsG0180001689.01.T01:CDS
TCTGAAGCTTAGGACAGTTT+TGG	0.313110	1:+25345688	None:intergenic
GTTTAATGGCTTACACAAAT+TGG	0.321294	1:-25345985	MsG0180001689.01.T01:CDS
TTTGTTGTTGACAGGATTAT+AGG	0.322117	1:-25345582	MsG0180001689.01.T01:intron
ATCTCCATGTGAGTCAAATT+AGG	0.339910	1:+25345765	None:intergenic
TATAGGCATAGACGTGAAAT+TGG	0.346426	1:-25345565	MsG0180001689.01.T01:CDS
GGAACTTGGTAGTCTTCATA+TGG	0.362370	1:+25345795	None:intergenic
CAGCTTTGCGCGTATAAACT+TGG	0.367725	1:+25345905	None:intergenic
TTGGAAGGTTCCGTTTGATT+TGG	0.373474	1:-25345937	MsG0180001689.01.T01:CDS
AAGGCCGATAGAGAGTTTAA+TGG	0.375262	1:-25345999	MsG0180001689.01.T01:CDS
TGCTCGCTTATAGGTTATAA+AGG	0.381379	1:-25345481	MsG0180001689.01.T01:CDS
GGTGGACCCACTAATTGTTT+CGG	0.400570	1:-25345530	MsG0180001689.01.T01:CDS
CAGATGAATGGTTTGGGAAG+TGG	0.404081	1:-25345731	MsG0180001689.01.T01:CDS
GTTTGCAATTCCTCTAAAAT+GGG	0.414647	1:+25346047	None:intergenic
CTTAACTGTTCTTAAGTTGA+AGG	0.414815	1:-25346189	MsG0180001689.01.T01:CDS
TTGATACAGATGAATGGTTT+GGG	0.419756	1:-25345737	MsG0180001689.01.T01:CDS
TTTGATACAGATGAATGGTT+TGG	0.420436	1:-25345738	MsG0180001689.01.T01:CDS
TAGAGCAAATATTATGAACT+TGG	0.422128	1:-25345961	MsG0180001689.01.T01:CDS
AGTCTTCGGATTCTCCTCTA+TGG	0.430649	1:-25346074	MsG0180001689.01.T01:CDS
CAAGTTTATACGCGCAAAGC+TGG	0.441719	1:-25345904	MsG0180001689.01.T01:intron
TGCAAACAGATGGTATATTC+TGG	0.442448	1:-25346031	MsG0180001689.01.T01:CDS
ACAGCATTGGTAGCAGTCTT+CGG	0.442909	1:-25346088	MsG0180001689.01.T01:CDS
CAAATATTATGAACTTGGGT+TGG	0.446882	1:-25345956	MsG0180001689.01.T01:CDS
GCTCAAAACTCTTCATTTGG+AGG	0.452730	1:-25346126	MsG0180001689.01.T01:CDS
CTTTCCTAATTTGACTCACA+TGG	0.460924	1:-25345769	MsG0180001689.01.T01:CDS
ACTTAAAGCTATAACCCCTA+TGG	0.470337	1:+25346240	None:intergenic
TATTATGAACTTGGGTTGGA+AGG	0.470738	1:-25345952	MsG0180001689.01.T01:CDS
TTAACTGTTCTTAAGTTGAA+GGG	0.472566	1:-25346188	MsG0180001689.01.T01:CDS
ATAGTAAGACTCTGAAGCTT+AGG	0.477351	1:+25345678	None:intergenic
ATTCATTTCAATTACAGCAT+TGG	0.489996	1:-25346101	MsG0180001689.01.T01:CDS
CAACATCGACATGTCCCATA+GGG	0.499837	1:-25346255	None:intergenic
TGAAATTGGCGATAACAATT+GGG	0.501322	1:-25345551	MsG0180001689.01.T01:CDS
TTGTATTTGATACAGATGAA+TGG	0.512834	1:-25345743	MsG0180001689.01.T01:CDS
AATTAGGAAAGGTGGGAACT+TGG	0.513063	1:+25345781	None:intergenic
GACAATTAATGCCTCTCTCA+TGG	0.513096	1:-25345395	MsG0180001689.01.T01:CDS
AGAGCAAATATTATGAACTT+GGG	0.524643	1:-25345960	MsG0180001689.01.T01:CDS
TATATTCTGGCTGCATAAGA+AGG	0.525563	1:-25346018	MsG0180001689.01.T01:CDS
TTAGAGGAATTGCAAACAGA+TGG	0.534856	1:-25346041	MsG0180001689.01.T01:CDS
GTGAGTCAAATTAGGAAAGG+TGG	0.536674	1:+25345773	None:intergenic
AGACATTCCGAAACAATTAG+TGG	0.543202	1:+25345523	None:intergenic
GCGTTACGAACCAAATCAAA+CGG	0.548435	1:+25345927	None:intergenic
TGAGTCAAATTAGGAAAGGT+GGG	0.558768	1:+25345774	None:intergenic
CTAAAATGGGACAGCCATAG+AGG	0.569602	1:+25346060	None:intergenic
CTTAAAGCTATAACCCCTAT+GGG	0.570808	1:+25346241	None:intergenic
TAACTGTTCTTAAGTTGAAG+GGG	0.572427	1:-25346187	MsG0180001689.01.T01:CDS
TAAGCCATTAAACTCTCTAT+CGG	0.575796	1:+25345995	None:intergenic
TCAACATCGACATGTCCCAT+AGG	0.576127	1:-25346256	None:intergenic
GAACTTGGTAGTCTTCATAT+GGG	0.577680	1:+25345796	None:intergenic
TATTTATATCACCATGAGAG+AGG	0.600491	1:+25345384	None:intergenic
CTGCAACTGAAAACACAAAG+AGG	0.603645	1:+25346215	None:intergenic
CATGTGAGTCAAATTAGGAA+AGG	0.610877	1:+25345770	None:intergenic
GACATTCCGAAACAATTAGT+GGG	0.613572	1:+25345524	None:intergenic
TCAGAGTCTTACTATTCATG+AGG	0.621549	1:-25345670	MsG0180001689.01.T01:intron
GAGAGAGGCATTAATTGTCA+TGG	0.622785	1:+25345399	None:intergenic
AATTGGCGATAACAATTGGG+TGG	0.649089	1:-25345548	MsG0180001689.01.T01:CDS
AACATCGACATGTCCCATAG+GGG	0.684223	1:-25346254	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAAAACAAACATTAGAAAT+AGG	+	Chr1:25345799-25345818	None:intergenic	20.0%
!!!	AACAATTTTCTAAGAACAAA+TGG	+	Chr1:25346032-25346051	None:intergenic	20.0%
!	AGAGCAAATATTATGAACTT+GGG	-	Chr1:25345672-25345691	MsG0180001689.01.T01:CDS	25.0%
!	ATAGGCCAAATTTGTATTAA+CGG	+	Chr1:25345781-25345800	None:intergenic	25.0%
!	ATTCATTTCAATTACAGCAT+TGG	-	Chr1:25345531-25345550	MsG0180001689.01.T01:CDS	25.0%
!	TAGAGCAAATATTATGAACT+TGG	-	Chr1:25345671-25345690	MsG0180001689.01.T01:CDS	25.0%
!	TTGTATTTGATACAGATGAA+TGG	-	Chr1:25345889-25345908	MsG0180001689.01.T01:intron	25.0%
!!!	TTAACTGTTCTTAAGTTGAA+GGG	-	Chr1:25345444-25345463	MsG0180001689.01.T01:CDS	25.0%
	CAAATATTATGAACTTGGGT+TGG	-	Chr1:25345676-25345695	MsG0180001689.01.T01:CDS	30.0%
	GTTTAATGGCTTACACAAAT+TGG	-	Chr1:25345647-25345666	MsG0180001689.01.T01:intron	30.0%
	TAAGCCATTAAACTCTCTAT+CGG	+	Chr1:25345640-25345659	None:intergenic	30.0%
	TAGGCCAAATTTGTATTAAC+GGG	+	Chr1:25345780-25345799	None:intergenic	30.0%
	TGAAATTGGCGATAACAATT+GGG	-	Chr1:25346081-25346100	MsG0180001689.01.T01:CDS	30.0%
	TTGATACAGATGAATGGTTT+GGG	-	Chr1:25345895-25345914	MsG0180001689.01.T01:intron	30.0%
	TTTGATACAGATGAATGGTT+TGG	-	Chr1:25345894-25345913	MsG0180001689.01.T01:intron	30.0%
!	GTTTTTGTAATTCATGCAGA+AGG	-	Chr1:25345812-25345831	MsG0180001689.01.T01:intron	30.0%
!	TGTTTGCAATTCCTCTAAAA+TGG	+	Chr1:25345589-25345608	None:intergenic	30.0%
!!	CTTAACTGTTCTTAAGTTGA+AGG	-	Chr1:25345443-25345462	MsG0180001689.01.T01:CDS	30.0%
!!	GAAAATTGTTTGTTGTTGAC+AGG	-	Chr1:25346042-25346061	MsG0180001689.01.T01:CDS	30.0%
!!	GTTTGCAATTCCTCTAAAAT+GGG	+	Chr1:25345588-25345607	None:intergenic	30.0%
!!	TAACTGTTCTTAAGTTGAAG+GGG	-	Chr1:25345445-25345464	MsG0180001689.01.T01:CDS	30.0%
!!	TTTGTTGTTGACAGGATTAT+AGG	-	Chr1:25346050-25346069	MsG0180001689.01.T01:CDS	30.0%
!!!	AAATGAAGAGTTTTGAGCAA+GGG	+	Chr1:25345504-25345523	None:intergenic	30.0%
	ACTTAAAGCTATAACCCCTA+TGG	+	Chr1:25345395-25345414	None:intergenic	35.0%
	AGACATTCCGAAACAATTAG+TGG	+	Chr1:25346112-25346131	None:intergenic	35.0%
	ATCTCCATGTGAGTCAAATT+AGG	+	Chr1:25345870-25345889	None:intergenic	35.0%
	CATGTGAGTCAAATTAGGAA+AGG	+	Chr1:25345865-25345884	None:intergenic	35.0%
	CTTAAAGCTATAACCCCTAT+GGG	+	Chr1:25345394-25345413	None:intergenic	35.0%
	CTTGCTCAAAACTCTTCATT+TGG	-	Chr1:25345503-25345522	MsG0180001689.01.T01:CDS	35.0%
	CTTTCCTAATTTGACTCACA+TGG	-	Chr1:25345863-25345882	MsG0180001689.01.T01:intron	35.0%
	GACATTCCGAAACAATTAGT+GGG	+	Chr1:25346111-25346130	None:intergenic	35.0%
	GTGAAATTGGCGATAACAAT+TGG	-	Chr1:25346080-25346099	MsG0180001689.01.T01:CDS	35.0%
	TATAGGCATAGACGTGAAAT+TGG	-	Chr1:25346067-25346086	MsG0180001689.01.T01:CDS	35.0%
	TCAGAGTCTTACTATTCATG+AGG	-	Chr1:25345962-25345981	MsG0180001689.01.T01:CDS	35.0%
	TCCTCCCGTTAATACAAATT+TGG	-	Chr1:25345773-25345792	MsG0180001689.01.T01:CDS	35.0%
	TGAGTCAAATTAGGAAAGGT+GGG	+	Chr1:25345861-25345880	None:intergenic	35.0%
	TGCAAACAGATGGTATATTC+TGG	-	Chr1:25345601-25345620	MsG0180001689.01.T01:intron	35.0%
	TGCTCGCTTATAGGTTATAA+AGG	-	Chr1:25346151-25346170	MsG0180001689.01.T01:CDS	35.0%
	TTAGAGGAATTGCAAACAGA+TGG	-	Chr1:25345591-25345610	MsG0180001689.01.T01:intron	35.0%
!	GAACTTGGTAGTCTTCATAT+GGG	+	Chr1:25345839-25345858	None:intergenic	35.0%
!	TATATTCTGGCTGCATAAGA+AGG	-	Chr1:25345614-25345633	MsG0180001689.01.T01:intron	35.0%
!!	ATAGTAAGACTCTGAAGCTT+AGG	+	Chr1:25345957-25345976	None:intergenic	35.0%
!!	TATTATGAACTTGGGTTGGA+AGG	-	Chr1:25345680-25345699	MsG0180001689.01.T01:CDS	35.0%
!!	TATTCATGAGGTCTTATTCG+CGG	-	Chr1:25345974-25345993	MsG0180001689.01.T01:CDS	35.0%
!!!	CAAATGAAGAGTTTTGAGCA+AGG	+	Chr1:25345505-25345524	None:intergenic	35.0%
	AAGGCCGATAGAGAGTTTAA+TGG	-	Chr1:25345633-25345652	MsG0180001689.01.T01:intron	40.0%
	AATTAGGAAAGGTGGGAACT+TGG	+	Chr1:25345854-25345873	None:intergenic	40.0%
	AATTGGCGATAACAATTGGG+TGG	-	Chr1:25346084-25346103	MsG0180001689.01.T01:CDS	40.0%
	CTGCAACTGAAAACACAAAG+AGG	+	Chr1:25345420-25345439	None:intergenic	40.0%
	CTTAGAATGTGCTCGCTTAT+AGG	-	Chr1:25346142-25346161	MsG0180001689.01.T01:CDS	40.0%
	GACAATTAATGCCTCTCTCA+TGG	-	Chr1:25346237-25346256	MsG0180001689.01.T01:CDS	40.0%
	GCCAAATTTGTATTAACGGG+AGG	+	Chr1:25345777-25345796	None:intergenic	40.0%
	GCGTTACGAACCAAATCAAA+CGG	+	Chr1:25345708-25345727	None:intergenic	40.0%
	GCTCAAAACTCTTCATTTGG+AGG	-	Chr1:25345506-25345525	MsG0180001689.01.T01:CDS	40.0%
	GGTATGAACATTGAACACCA+TGG	-	Chr1:25345749-25345768	MsG0180001689.01.T01:CDS	40.0%
	GTGAGTCAAATTAGGAAAGG+TGG	+	Chr1:25345862-25345881	None:intergenic	40.0%
	TTGGAAGGTTCCGTTTGATT+TGG	-	Chr1:25345695-25345714	MsG0180001689.01.T01:CDS	40.0%
!	GGAACTTGGTAGTCTTCATA+TGG	+	Chr1:25345840-25345859	None:intergenic	40.0%
!!	GAGAGAGGCATTAATTGTCA+TGG	+	Chr1:25346236-25346255	None:intergenic	40.0%
!!	TCTGAAGCTTAGGACAGTTT+TGG	+	Chr1:25345947-25345966	None:intergenic	40.0%
	AGTCTTCGGATTCTCCTCTA+TGG	-	Chr1:25345558-25345577	MsG0180001689.01.T01:CDS	45.0%
	CAAGTTTATACGCGCAAAGC+TGG	-	Chr1:25345728-25345747	MsG0180001689.01.T01:CDS	45.0%
	CAGCTTTGCGCGTATAAACT+TGG	+	Chr1:25345730-25345749	None:intergenic	45.0%
	CTAAAATGGGACAGCCATAG+AGG	+	Chr1:25345575-25345594	None:intergenic	45.0%
	GGTGGACCCACTAATTGTTT+CGG	-	Chr1:25346102-25346121	MsG0180001689.01.T01:CDS	45.0%
!	CAGATGAATGGTTTGGGAAG+TGG	-	Chr1:25345901-25345920	MsG0180001689.01.T01:intron	45.0%
!	CTATGGCTGTCCCATTTTAG+AGG	-	Chr1:25345575-25345594	MsG0180001689.01.T01:intron	45.0%
!!	ACAGCATTGGTAGCAGTCTT+CGG	-	Chr1:25345544-25345563	MsG0180001689.01.T01:CDS	45.0%
	TGTATTAACGGGAGGAGCCA+TGG	+	Chr1:25345769-25345788	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	25345387	25346267	25345387	ID=MsG0180001689.01;Name=MsG0180001689.01
Chr1	mRNA	25345387	25346267	25345387	ID=MsG0180001689.01.T01;Parent=MsG0180001689.01;Name=MsG0180001689.01.T01;_AED=0.22;_eAED=0.22;_QI=0\|0\|0\|1\|1\|1\|3\|0\|239
Chr1	exon	25345905	25346267	25345905	ID=MsG0180001689.01.T01:exon:18428;Parent=MsG0180001689.01.T01
Chr1	exon	25345671	25345823	25345671	ID=MsG0180001689.01.T01:exon:18427;Parent=MsG0180001689.01.T01
Chr1	exon	25345387	25345590	25345387	ID=MsG0180001689.01.T01:exon:18426;Parent=MsG0180001689.01.T01
Chr1	CDS	25345905	25346267	25345905	ID=MsG0180001689.01.T01:cds;Parent=MsG0180001689.01.T01
Chr1	CDS	25345671	25345823	25345671	ID=MsG0180001689.01.T01:cds;Parent=MsG0180001689.01.T01
Chr1	CDS	25345387	25345590	25345387	ID=MsG0180001689.01.T01:cds;Parent=MsG0180001689.01.T01

Gene Sequence

>MsG0180001689.01.T01

ATGTCCCATAGGGGTTATAGCTTTAAGTTACCTCTTTGTGTTTTCAGTTGCAGTAACTTAACTGTTCTTAAGTTGAAGGGGATAAAAATGCATGAACTTTTTCATGTCAATTTTCCCTTGCTCAAAACTCTTCATTTGGAGGCAATTCATTTCAATTACAGCATTGGTAGCAGTCTTCGGATTCTCCTCTATGGCTGTCCCATTTTAGAGGAATTGCAAACAGATGGTATATTCTGGCTGCATAAGAAGGCCGATAGAGAGTTTAATGGCTTACACAAATTGGTTAGAGCAAATATTATGAACTTGGGTTGGAAGGTTCCGTTTGATTTGGTTCGTAACGCCAAGTTTATACGCGCAAAGCTGAAGGACCCATATGAAGACTACCAAGTTCCCACCTTTCCTAATTTGACTCACATGGAGATTGTATTTGATACAGATGAATGGTTTGGGAAGTGGAAGTTTTTGACTGAAGTGCTCCAAAACTGTCCTAAGCTTCAGAGTCTTACTATTCATGAGGATTATAGGCATAGACGTGAAATTGGCGATAACAATTGGGTGGACCCACTAATTGTTTCGGAATGTCTTTCATCTCAGCTTAGAATGTGCTCGCTTATAGGTTATAAAGGCATGAAGTGTGAGCTTCAGTTCGCAGAGTATATTTTAAAGAATGCAAAAGTATTGCACACCATGACAATTAATGCCTCTCTCATGGTGATATAA

Protein sequence

>MsG0180001689.01.T01

MSHRGYSFKLPLCVFSCSNLTVLKLKGIKMHELFHVNFPLLKTLHLEAIHFNYSIGSSLRILLYGCPILEELQTDGIFWLHKKADREFNGLHKLVRANIMNLGWKVPFDLVRNAKFIRAKLKDPYEDYQVPTFPNLTHMEIVFDTDEWFGKWKFLTEVLQNCPKLQSLTIHEDYRHRREIGDNNWVDPLIVSECLSSQLRMCSLIGYKGMKCELQFAEYILKNAKVLHTMTINASLMVI*