Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038156.01.T01 | AES73648.2 | 70.588 | 238 | 69 | 1 | 1 | 237 | 58 | 295 | 2.75E-107 | 325 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038156.01.T01 | G7JB20 | 70.588 | 238 | 69 | 1 | 1 | 237 | 58 | 295 | 1.31e-107 | 325 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038156.01.T01 | MTR_3g107160 | 70.588 | 238 | 69 | 1 | 1 | 237 | 58 | 295 | 3.33e-111 | 325 |
| MsG0780038156.01.T01 | MTR_3g107120 | 57.080 | 226 | 94 | 2 | 10 | 235 | 79 | 301 | 1.20e-76 | 237 |
| MsG0780038156.01.T01 | MTR_3g107100 | 54.430 | 237 | 103 | 3 | 4 | 238 | 63 | 296 | 7.00e-73 | 228 |
| MsG0780038156.01.T01 | MTR_3g107140 | 55.629 | 151 | 64 | 2 | 1 | 151 | 50 | 197 | 1.41e-46 | 154 |
| MsG0780038156.01.T01 | MTR_2g007450 | 42.803 | 264 | 117 | 9 | 5 | 237 | 69 | 329 | 2.56e-46 | 159 |
| MsG0780038156.01.T01 | MTR_2g007400 | 40.800 | 250 | 130 | 8 | 2 | 238 | 62 | 306 | 3.80e-40 | 142 |
| MsG0780038156.01.T01 | MTR_1g053025 | 39.453 | 256 | 120 | 9 | 5 | 237 | 56 | 299 | 6.54e-39 | 139 |
| MsG0780038156.01.T01 | MTR_4g098590 | 40.574 | 244 | 132 | 8 | 5 | 238 | 52 | 292 | 9.04e-37 | 133 |
| MsG0780038156.01.T01 | MTR_2g007500 | 37.302 | 252 | 121 | 6 | 1 | 237 | 63 | 292 | 1.17e-35 | 129 |
| MsG0780038156.01.T01 | MTR_3g106960 | 61.468 | 109 | 39 | 2 | 132 | 237 | 1 | 109 | 1.70e-35 | 126 |
| MsG0780038156.01.T01 | MTR_5g083890 | 36.187 | 257 | 138 | 9 | 1 | 237 | 55 | 305 | 2.36e-31 | 120 |
| MsG0780038156.01.T01 | MTR_3g450920 | 50.649 | 154 | 61 | 5 | 1 | 153 | 62 | 201 | 6.28e-30 | 111 |
| MsG0780038156.01.T01 | MTR_1g107385 | 38.735 | 253 | 134 | 11 | 1 | 237 | 81 | 328 | 3.70e-28 | 110 |
| MsG0780038156.01.T01 | MTR_3g099830 | 34.426 | 244 | 124 | 6 | 1 | 237 | 61 | 275 | 2.76e-27 | 107 |
| MsG0780038156.01.T01 | MTR_3g026920 | 33.955 | 268 | 119 | 12 | 5 | 235 | 72 | 318 | 2.14e-26 | 105 |
| MsG0780038156.01.T01 | MTR_7g075820 | 35.271 | 258 | 140 | 7 | 1 | 238 | 55 | 305 | 4.05e-26 | 105 |
| MsG0780038156.01.T01 | MTR_2g005990 | 33.871 | 248 | 144 | 7 | 1 | 235 | 55 | 295 | 9.09e-24 | 98.6 |
| MsG0780038156.01.T01 | MTR_2g008320 | 34.959 | 246 | 136 | 9 | 1 | 234 | 101 | 334 | 1.77e-22 | 95.5 |
| MsG0780038156.01.T01 | MTR_7g075800 | 32.959 | 267 | 140 | 11 | 4 | 238 | 60 | 319 | 3.23e-22 | 95.5 |
| MsG0780038156.01.T01 | MTR_2g008310 | 32.653 | 245 | 141 | 5 | 1 | 235 | 51 | 281 | 4.39e-22 | 94.0 |
| MsG0780038156.01.T01 | MTR_7g083740 | 32.922 | 243 | 117 | 8 | 1 | 235 | 66 | 270 | 1.31e-20 | 89.7 |
| MsG0780038156.01.T01 | MTR_2g007360 | 38.983 | 177 | 93 | 7 | 70 | 238 | 102 | 271 | 3.88e-20 | 88.2 |
| MsG0780038156.01.T01 | MTR_1g041245 | 30.769 | 234 | 145 | 7 | 10 | 235 | 66 | 290 | 8.90e-18 | 81.6 |
| MsG0780038156.01.T01 | MTR_7g098510 | 34.010 | 197 | 119 | 5 | 46 | 235 | 105 | 297 | 9.70e-18 | 81.6 |
| MsG0780038156.01.T01 | MTR_7g007340 | 32.340 | 235 | 112 | 9 | 10 | 237 | 68 | 262 | 2.01e-17 | 80.5 |
| MsG0780038156.01.T01 | MTR_7g007080 | 30.213 | 235 | 151 | 7 | 10 | 238 | 62 | 289 | 1.93e-16 | 77.8 |
| MsG0780038156.01.T01 | MTR_5g073420 | 34.010 | 197 | 115 | 6 | 40 | 233 | 100 | 284 | 3.07e-16 | 77.4 |
| MsG0780038156.01.T01 | MTR_3g108150 | 32.218 | 239 | 140 | 7 | 9 | 231 | 56 | 288 | 4.54e-16 | 77.0 |
| MsG0780038156.01.T01 | MTR_1g108800 | 34.021 | 194 | 99 | 8 | 49 | 228 | 68 | 246 | 5.24e-16 | 76.3 |
| MsG0780038156.01.T01 | MTR_7g007370 | 37.952 | 166 | 93 | 5 | 75 | 237 | 93 | 251 | 7.98e-16 | 75.9 |
| MsG0780038156.01.T01 | MTR_3g071850 | 29.762 | 252 | 140 | 11 | 5 | 235 | 79 | 314 | 5.27e-15 | 74.3 |
| MsG0780038156.01.T01 | MTR_6g045333 | 29.528 | 254 | 142 | 11 | 5 | 237 | 116 | 353 | 2.77e-14 | 72.0 |
| MsG0780038156.01.T01 | MTR_6g011460 | 31.224 | 237 | 146 | 7 | 2 | 231 | 96 | 322 | 3.97e-14 | 71.2 |
| MsG0780038156.01.T01 | MTR_7g098570 | 35.052 | 194 | 101 | 11 | 54 | 233 | 115 | 297 | 5.01e-14 | 71.2 |
| MsG0780038156.01.T01 | MTR_6g029350 | 42.105 | 114 | 58 | 4 | 125 | 237 | 1 | 107 | 5.95e-14 | 69.3 |
| MsG0780038156.01.T01 | MTR_4g039440 | 36.686 | 169 | 87 | 9 | 77 | 231 | 151 | 313 | 2.68e-13 | 68.9 |
| MsG0780038156.01.T01 | MTR_5g076150 | 34.184 | 196 | 99 | 9 | 61 | 231 | 10 | 200 | 8.60e-13 | 66.2 |
| MsG0780038156.01.T01 | MTR_3g023630 | 29.661 | 236 | 136 | 13 | 10 | 232 | 63 | 281 | 9.92e-13 | 67.0 |
| MsG0780038156.01.T01 | MTR_7g026500 | 31.034 | 203 | 109 | 11 | 50 | 233 | 111 | 301 | 1.01e-12 | 67.0 |
| MsG0780038156.01.T01 | MTR_1g085120 | 30.435 | 253 | 152 | 11 | 1 | 239 | 228 | 470 | 1.57e-12 | 66.6 |
| MsG0780038156.01.T01 | MTR_5g073340 | 30.058 | 173 | 112 | 2 | 45 | 211 | 74 | 243 | 7.49e-12 | 64.3 |
| MsG0780038156.01.T01 | MTR_1g085120 | 31.050 | 219 | 132 | 9 | 2 | 210 | 142 | 351 | 7.96e-12 | 64.7 |
| MsG0780038156.01.T01 | MTR_5g007410 | 33.508 | 191 | 102 | 8 | 67 | 237 | 132 | 317 | 8.44e-12 | 64.7 |
| MsG0780038156.01.T01 | MTR_1g085120 | 30.909 | 220 | 133 | 9 | 1 | 210 | 216 | 426 | 8.65e-12 | 64.7 |
| MsG0780038156.01.T01 | MTR_7g088260 | 34.177 | 158 | 96 | 4 | 55 | 210 | 187 | 338 | 8.71e-12 | 64.7 |
| MsG0780038156.01.T01 | MTR_0543s0010 | 30.542 | 203 | 117 | 8 | 45 | 238 | 172 | 359 | 1.19e-11 | 64.3 |
| MsG0780038156.01.T01 | MTR_0492s0020 | 30.542 | 203 | 117 | 8 | 45 | 238 | 172 | 359 | 1.23e-11 | 63.9 |
| MsG0780038156.01.T01 | MTR_8g085430 | 34.000 | 150 | 91 | 3 | 10 | 152 | 66 | 214 | 1.44e-11 | 63.5 |
| MsG0780038156.01.T01 | MTR_5g045510 | 33.523 | 176 | 85 | 8 | 49 | 208 | 106 | 265 | 1.85e-11 | 63.5 |
| MsG0780038156.01.T01 | MTR_0009s0080 | 31.683 | 202 | 110 | 8 | 50 | 235 | 117 | 306 | 2.10e-11 | 63.5 |
| MsG0780038156.01.T01 | MTR_5g061680 | 35.616 | 146 | 77 | 6 | 3 | 138 | 57 | 195 | 2.42e-11 | 62.8 |
| MsG0780038156.01.T01 | MTR_6g022360 | 33.540 | 161 | 94 | 7 | 80 | 231 | 154 | 310 | 2.86e-11 | 62.8 |
| MsG0780038156.01.T01 | MTR_5g031970 | 29.293 | 198 | 107 | 8 | 49 | 239 | 97 | 268 | 3.12e-11 | 62.8 |
| MsG0780038156.01.T01 | MTR_5g058100 | 32.738 | 168 | 100 | 7 | 50 | 211 | 106 | 266 | 4.30e-11 | 62.4 |
| MsG0780038156.01.T01 | MTR_7g055943 | 28.870 | 239 | 146 | 8 | 5 | 231 | 93 | 319 | 4.61e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGGAGGGGAGTTTAA+TGG | 0.207407 | 7:+41321607 | None:intergenic |
| GAGTTGAGTGCCTTGAAATT+TGG | 0.253194 | 7:-41321736 | MsG0780038156.01.T01:CDS |
| CTGCAACTGAGAATGCTAAT+AGG | 0.292060 | 7:+41321680 | None:intergenic |
| GATTCTCATCTTTGTCATCT+TGG | 0.312679 | 7:-41321803 | MsG0780038156.01.T01:CDS |
| TCAAGATTTACTATTGCTTC+TGG | 0.313985 | 7:-41321549 | MsG0780038156.01.T01:CDS |
| TATGCTTTCAAGCTCTTGAT+AGG | 0.341897 | 7:+41321460 | None:intergenic |
| GTCGATGACGCAACTCTTGA+TGG | 0.343238 | 7:+41321838 | None:intergenic |
| CAAGTTGACGAAAGCAGCTT+TGG | 0.371712 | 7:-41321429 | MsG0780038156.01.T01:CDS |
| GCTTTCATAATGTCATCAAA+TGG | 0.422340 | 7:-41321781 | MsG0780038156.01.T01:CDS |
| AAACTCATTAAAGCGAAAAC+AGG | 0.424924 | 7:+41321892 | None:intergenic |
| ATTGTGCATAGAAATAAATA+AGG | 0.425065 | 7:-41321357 | MsG0780038156.01.T01:intron |
| TAAAGCGAAAACAGGTTTCT+CGG | 0.432381 | 7:+41321900 | None:intergenic |
| ACATTATGAAGTGCTTTCAA+TGG | 0.440936 | 7:+41321386 | None:intergenic |
| TGCAACTGAGAATGCTAATA+GGG | 0.446200 | 7:+41321681 | None:intergenic |
| GATTTACTATTGCTTCTGGC+TGG | 0.463222 | 7:-41321545 | MsG0780038156.01.T01:CDS |
| ATTTGGACTGGCGTGATTGA+TGG | 0.463271 | 7:-41321719 | MsG0780038156.01.T01:CDS |
| CTTTCATAATGTCATCAAAT+GGG | 0.477296 | 7:-41321780 | MsG0780038156.01.T01:CDS |
| TCAAAATCTTGAGCTCTGTC+AGG | 0.499115 | 7:-41321121 | MsG0780038156.01.T01:CDS |
| GCATGTAAATCCTCAAGAAT+CGG | 0.513909 | 7:+41321518 | None:intergenic |
| GTTCTATAAGTTCATTGTGA+AGG | 0.515687 | 7:-41321639 | MsG0780038156.01.T01:CDS |
| GGCTGGATGTCCGATTCTTG+AGG | 0.521513 | 7:-41321528 | MsG0780038156.01.T01:CDS |
| CATCAAATGGGTCAATGATC+TGG | 0.522069 | 7:-41321768 | MsG0780038156.01.T01:CDS |
| TCACGCCAGTCCAAATTTCA+AGG | 0.532109 | 7:+41321726 | None:intergenic |
| ATTGAAAGCACTTCATAATG+TGG | 0.544242 | 7:-41321384 | MsG0780038156.01.T01:CDS |
| GAGTGCCTTGAAATTTGGAC+TGG | 0.561925 | 7:-41321731 | MsG0780038156.01.T01:CDS |
| AATTGATAGAGTAAAGTCCC+AGG | 0.584232 | 7:+41321190 | None:intergenic |
| TTATTTGTTTAGGTGTATCG+AGG | 0.619267 | 7:-41321249 | MsG0780038156.01.T01:intron |
| TATAGAACTTGAGCACAACA+AGG | 0.622954 | 7:+41321654 | None:intergenic |
| AGGTAAGAGACTCTTTAGAG+TGG | 0.633754 | 7:+41321480 | None:intergenic |
| ATAGAACTTGAGCACAACAA+GGG | 0.692453 | 7:+41321655 | None:intergenic |
| GTCAATGATCTGGTGCAACG+CGG | 0.702850 | 7:-41321758 | MsG0780038156.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATAGAAATATTTAAATA+AGG | + | Chr7:41321750-41321769 | None:intergenic | 10.0% |
| !!! | TTCAAAATAAAGAATTTTGA+GGG | + | Chr7:41321432-41321451 | None:intergenic | 15.0% |
| !!! | TTTTTTAAATTATGGAAGAT+AGG | + | Chr7:41321811-41321830 | None:intergenic | 15.0% |
| !! | ATTGTGCATAGAAATAAATA+AGG | - | Chr7:41321664-41321683 | MsG0780038156.01.T01:CDS | 20.0% |
| !! | TTCCATAATTTAAAAAACCT+GGG | - | Chr7:41321814-41321833 | MsG0780038156.01.T01:CDS | 20.0% |
| !!! | CTTCAAAATAAAGAATTTTG+AGG | + | Chr7:41321433-41321452 | None:intergenic | 20.0% |
| !!! | TCTATGTTGTTTATTTGTTT+AGG | - | Chr7:41321762-41321781 | MsG0780038156.01.T01:CDS | 20.0% |
| ! | CTTCCATAATTTAAAAAACC+TGG | - | Chr7:41321813-41321832 | MsG0780038156.01.T01:CDS | 25.0% |
| ! | CTTTCATAATGTCATCAAAT+GGG | - | Chr7:41321241-41321260 | MsG0780038156.01.T01:intron | 25.0% |
| !!! | AAAATAAAGAATTTTGAGGG+AGG | + | Chr7:41321429-41321448 | None:intergenic | 25.0% |
| !!! | AAATAAAGAATTTTGAGGGA+GGG | + | Chr7:41321428-41321447 | None:intergenic | 25.0% |
| !!! | ACTTGATTTGAATTTTCTTG+GGG | - | Chr7:41321714-41321733 | MsG0780038156.01.T01:CDS | 25.0% |
| !!! | GACTTGATTTGAATTTTCTT+GGG | - | Chr7:41321713-41321732 | MsG0780038156.01.T01:CDS | 25.0% |
| !!! | TGACTTGATTTGAATTTTCT+TGG | - | Chr7:41321712-41321731 | MsG0780038156.01.T01:CDS | 25.0% |
| AAACTCATTAAAGCGAAAAC+AGG | + | Chr7:41321132-41321151 | None:intergenic | 30.0% | |
| GCTTTCATAATGTCATCAAA+TGG | - | Chr7:41321240-41321259 | MsG0780038156.01.T01:CDS | 30.0% | |
| GTTCTATAAGTTCATTGTGA+AGG | - | Chr7:41321382-41321401 | MsG0780038156.01.T01:CDS | 30.0% | |
| TCAAGATTTACTATTGCTTC+TGG | - | Chr7:41321472-41321491 | MsG0780038156.01.T01:CDS | 30.0% | |
| TTATTTGTTTAGGTGTATCG+AGG | - | Chr7:41321772-41321791 | MsG0780038156.01.T01:CDS | 30.0% | |
| ! | ACATTATGAAGTGCTTTCAA+TGG | + | Chr7:41321638-41321657 | None:intergenic | 30.0% |
| !! | AATAAAGAATTTTGAGGGAG+GGG | + | Chr7:41321427-41321446 | None:intergenic | 30.0% |
| !! | ATTGAAAGCACTTCATAATG+TGG | - | Chr7:41321637-41321656 | MsG0780038156.01.T01:CDS | 30.0% |
| !! | CTTGATTTGAATTTTCTTGG+GGG | - | Chr7:41321715-41321734 | MsG0780038156.01.T01:CDS | 30.0% |
| !! | TTGATTTGAATTTTCTTGGG+GGG | - | Chr7:41321716-41321735 | MsG0780038156.01.T01:CDS | 30.0% |
| !!! | GTCCCAGGTTTTTTAAATTA+TGG | + | Chr7:41321819-41321838 | None:intergenic | 30.0% |
| AATTGATAGAGTAAAGTCCC+AGG | + | Chr7:41321834-41321853 | None:intergenic | 35.0% | |
| ATAGAACTTGAGCACAACAA+GGG | + | Chr7:41321369-41321388 | None:intergenic | 35.0% | |
| GATTCTCATCTTTGTCATCT+TGG | - | Chr7:41321218-41321237 | MsG0780038156.01.T01:CDS | 35.0% | |
| GCATGTAAATCCTCAAGAAT+CGG | + | Chr7:41321506-41321525 | None:intergenic | 35.0% | |
| TAAAGCGAAAACAGGTTTCT+CGG | + | Chr7:41321124-41321143 | None:intergenic | 35.0% | |
| TATAGAACTTGAGCACAACA+AGG | + | Chr7:41321370-41321389 | None:intergenic | 35.0% | |
| TATGCTTTCAAGCTCTTGAT+AGG | + | Chr7:41321564-41321583 | None:intergenic | 35.0% | |
| TGCAACTGAGAATGCTAATA+GGG | + | Chr7:41321343-41321362 | None:intergenic | 35.0% | |
| ! | AATTTTCTTGGGGGGAATTT+CGG | - | Chr7:41321724-41321743 | MsG0780038156.01.T01:CDS | 35.0% |
| !! | AGCTCAAGATTTTGAAGGTT+AGG | + | Chr7:41321895-41321914 | None:intergenic | 35.0% |
| !! | GCTCAAGATTTTGAAGGTTA+GGG | + | Chr7:41321894-41321913 | None:intergenic | 35.0% |
| CATCAAATGGGTCAATGATC+TGG | - | Chr7:41321253-41321272 | MsG0780038156.01.T01:intron | 40.0% | |
| CTGCAACTGAGAATGCTAAT+AGG | + | Chr7:41321344-41321363 | None:intergenic | 40.0% | |
| GAGTTGAGTGCCTTGAAATT+TGG | - | Chr7:41321285-41321304 | MsG0780038156.01.T01:intron | 40.0% | |
| GATTTACTATTGCTTCTGGC+TGG | - | Chr7:41321476-41321495 | MsG0780038156.01.T01:CDS | 40.0% | |
| TCAAAATCTTGAGCTCTGTC+AGG | - | Chr7:41321900-41321919 | MsG0780038156.01.T01:CDS | 40.0% | |
| ! | AGGTAAGAGACTCTTTAGAG+TGG | + | Chr7:41321544-41321563 | None:intergenic | 40.0% |
| ! | CGTCATCGACATTTTGTATG+CGG | - | Chr7:41321196-41321215 | MsG0780038156.01.T01:CDS | 40.0% |
| ! | GACAGAGCTCAAGATTTTGA+AGG | + | Chr7:41321900-41321919 | None:intergenic | 40.0% |
| GAGTGCCTTGAAATTTGGAC+TGG | - | Chr7:41321290-41321309 | MsG0780038156.01.T01:intron | 45.0% | |
| TCACGCCAGTCCAAATTTCA+AGG | + | Chr7:41321298-41321317 | None:intergenic | 45.0% | |
| TTTGAGGGAGGGGAGTTTAA+TGG | + | Chr7:41321417-41321436 | None:intergenic | 45.0% | |
| ! | CAAGTTGACGAAAGCAGCTT+TGG | - | Chr7:41321592-41321611 | MsG0780038156.01.T01:CDS | 45.0% |
| !! | ATTTGGACTGGCGTGATTGA+TGG | - | Chr7:41321302-41321321 | MsG0780038156.01.T01:intron | 45.0% |
| GTCAATGATCTGGTGCAACG+CGG | - | Chr7:41321263-41321282 | MsG0780038156.01.T01:intron | 50.0% | |
| GTCGATGACGCAACTCTTGA+TGG | + | Chr7:41321186-41321205 | None:intergenic | 50.0% | |
| GGCTGGATGTCCGATTCTTG+AGG | - | Chr7:41321493-41321512 | MsG0780038156.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 41321113 | 41321930 | 41321113 | ID=MsG0780038156.01;Name=MsG0780038156.01 |
| Chr7 | mRNA | 41321113 | 41321930 | 41321113 | ID=MsG0780038156.01.T01;Parent=MsG0780038156.01;Name=MsG0780038156.01.T01;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|1|1|2|0|239 |
| Chr7 | exon | 41321358 | 41321930 | 41321358 | ID=MsG0780038156.01.T01:exon:15447;Parent=MsG0780038156.01.T01 |
| Chr7 | exon | 41321113 | 41321259 | 41321113 | ID=MsG0780038156.01.T01:exon:15446;Parent=MsG0780038156.01.T01 |
| Chr7 | CDS | 41321358 | 41321930 | 41321358 | ID=MsG0780038156.01.T01:cds;Parent=MsG0780038156.01.T01 |
| Chr7 | CDS | 41321113 | 41321259 | 41321113 | ID=MsG0780038156.01.T01:cds;Parent=MsG0780038156.01.T01 |
| Gene Sequence |
| Protein sequence |
