AlfalfaGEDB | MsG0780037571.01

M. sativa cultivar ZhongmuNo.1 / MsG0780037571.01

KEGG: -
GO: -
PFAM: PF08387

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780037571.01.T01	PNX98124.1	59.283	307	116	6	1	304	69	369	5.95E-113	342

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780037571.01.T01	Q9LXJ7	28.716	296	167	12	39	304	101	382	4.88E-12	69.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780037571.01.T01	A0A2K3N552	59.283	307	116	6	1	304	69	369	2.84e-113	342

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080047972.01	MsG0780037571.01	PPI
MsG0080048094.01	MsG0780037571.01	PPI
MsG0080048156.01	MsG0780037571.01	PPI
MsG0080049065.01	MsG0780037571.01	PPI
MsG0180002225.01	MsG0780037571.01	PPI
MsG0180003609.01	MsG0780037571.01	PPI
MsG0180004709.01	MsG0780037571.01	PPI
MsG0180005259.01	MsG0780037571.01	PPI
MsG0180005886.01	MsG0780037571.01	PPI
MsG0180006146.01	MsG0780037571.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780037571.01.T01	MTR_3g107100	42.532	308	155	9	1	306	77	364	1.52e-64	209
MsG0780037571.01.T01	MTR_3g107120	44.027	293	146	7	10	300	93	369	9.37e-60	196
MsG0780037571.01.T01	MTR_3g107160	40.604	298	162	7	1	296	75	359	4.24e-58	192
MsG0780037571.01.T01	MTR_2g007450	38.941	321	148	13	1	289	82	386	3.44e-50	172
MsG0780037571.01.T01	MTR_2g007400	37.748	302	163	13	1	288	78	368	9.01e-47	162
MsG0780037571.01.T01	MTR_1g053025	37.908	306	152	13	1	288	69	354	5.18e-44	155
MsG0780037571.01.T01	MTR_1g107385	38.562	306	143	13	1	282	98	382	6.79e-44	155
MsG0780037571.01.T01	MTR_5g083890	38.111	307	160	12	1	288	72	367	9.04e-43	154
MsG0780037571.01.T01	MTR_2g008320	41.150	226	113	5	65	282	173	386	2.13e-40	146
MsG0780037571.01.T01	MTR_7g075820	35.811	296	154	10	10	288	83	359	4.41e-40	144

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780037571.01.T01	AT4G00160	28.428	299	155	11	39	288	109	397	4.72e-15	75.9
MsG0780037571.01.T01	AT4G26340	24.921	317	168	8	23	285	77	377	4.49e-14	72.8
MsG0780037571.01.T01	AT3G26920	26.241	282	162	10	28	288	6	262	1.42e-12	67.4
MsG0780037571.01.T01	AT5G22730	33.333	99	65	1	37	135	119	216	3.87e-11	63.9
MsG0780037571.01.T01	AT5G22730	33.333	99	65	1	37	135	119	216	3.87e-11	63.9
MsG0780037571.01.T01	AT3G26920	25.163	306	174	10	28	288	6	301	4.16e-11	63.5
MsG0780037571.01.T01	AT3G26920	25.163	306	174	10	28	288	6	301	4.16e-11	63.5
MsG0780037571.01.T01	AT5G22730	33.333	99	65	1	37	135	119	216	4.95e-11	63.5

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Find 63 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATCGACAAAGAACAT+TGG	0.547418	7:-28595601	MsG0780037571.01.T01:CDS
AAAATCCTCAGGTGTTCCTG+CGG	0.659666	7:+28595427	None:intergenic
AAACATCGACAAAGAACATT+GGG	0.486742	7:-28595600	MsG0780037571.01.T01:CDS
AAAGTATTAATTGGAAGTGC+GGG	0.547761	7:+28596572	None:intergenic
AAAGTGCTGACAGGGCCGTG+AGG	0.709366	7:+28596503	None:intergenic
AACATAAGATCTCGAAGTTT+AGG	0.267358	7:+28596266	None:intergenic
AACCGGATGTCCAATTCTTG+AGG	0.532154	7:-28596237	MsG0780037571.01.T01:CDS
AAGCGCAAATAAACACCAAA+TGG	0.433043	7:+28595899	None:intergenic
AAGGATATGTAACATCAAGG+TGG	0.697568	7:+28596549	None:intergenic
AAGTTCTTAGGTGTAATGAA+AGG	0.636997	7:+28595552	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACATCGACAAAGAACAT+TGG	-	Chr7:28596392-28596411	MsG0780037571.01.T01:CDS	30.0%
AAACATCGACAAAGAACATT+GGG	-	Chr7:28596393-28596412	MsG0780037571.01.T01:CDS	30.0%
AACATAAGATCTCGAAGTTT+AGG	+	Chr7:28595730-28595749	None:intergenic	30.0%
AAGTTCTTAGGTGTAATGAA+AGG	+	Chr7:28596444-28596463	None:intergenic	30.0%
ACATAAGATCTCGAAGTTTA+GGG	+	Chr7:28595729-28595748	None:intergenic	30.0%
ATAGCAATCAATATCAGCAT+AGG	+	Chr7:28595866-28595885	None:intergenic	30.0%
ATAGTTAACAAACCTCATTG+AGG	+	Chr7:28596187-28596206	None:intergenic	30.0%
ATTAAGTTTGAGAACCACAA+CGG	+	Chr7:28595629-28595648	None:intergenic	30.0%
CAAGTTATGAAAAGTAGGAA+TGG	+	Chr7:28596072-28596091	None:intergenic	30.0%
CATAAGGATATGTAACATCA+AGG	+	Chr7:28595450-28595469	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	28595406	28596609	28595406	ID=MsG0780037571.01;Name=MsG0780037571.01
Chr7	mRNA	28595406	28596609	28595406	ID=MsG0780037571.01.T01;Parent=MsG0780037571.01;Name=MsG0780037571.01.T01;_AED=0.16;_eAED=0.16;_QI=0\|0\|0\|1\|1\|1\|3\|0\|312
Chr7	exon	28596067	28596609	28596067	ID=MsG0780037571.01.T01:exon:9602;Parent=MsG0780037571.01.T01
Chr7	exon	28595822	28595965	28595822	ID=MsG0780037571.01.T01:exon:9601;Parent=MsG0780037571.01.T01
Chr7	exon	28595406	28595657	28595406	ID=MsG0780037571.01.T01:exon:9600;Parent=MsG0780037571.01.T01
Chr7	CDS	28596067	28596609	28596067	ID=MsG0780037571.01.T01:cds;Parent=MsG0780037571.01.T01
Chr7	CDS	28595822	28595965	28595822	ID=MsG0780037571.01.T01:cds;Parent=MsG0780037571.01.T01
Chr7	CDS	28595406	28595657	28595406	ID=MsG0780037571.01.T01:cds;Parent=MsG0780037571.01.T01

Gene Sequence

>MsG0780037571.01.T01

TTGCTCTCTCGAGATCCCGCACTTCCAATTAATACTTTCCACCTTGATGTTACATATCCTTATGATCTTGATCTTATTCTCGATCCTCACGGCCCTGTCAGCACTTTCACCAAATGGGTGAACTTTGTTGTACAACGTCGTGTTGAGTATCTTTATCTCTCAGTAGTATCATCGGGGCTCCAATATTCCAAATTGCCAATCAGTGTTATCACCTGCAGTACCGTTGTGGTTCTCAAACTTAATCGTTTTATTATTCAAGGCGGTTTTTCTCCAGTTTTACTTCCATCTCTTAAAACTCTTCATTTGGAAGATAGTTCTTTCCCTAAACTTCGAGATCTTATGTTGTTGTTAACCGGATGTCCAATTCTTGAGGATTTATTTACATTTAATGTGGTGTTTGATTCCGACGAATCTCTTACATGTGATGAGTGGAAGATCTTTTGCTTAAGTAACTTGACCTATGCTGATATTGATTGCTATCGTTTTCATTTTCCATTGGAAGCCGTTCACAATGTACCTACTTTGCGATTGGAAATTGACAAGGGTTATTGTCGTAATGATTCCATTCCTACTTTTCATAACTTGACCCATTTGGTGTTTATTTGCGCTTCTCTCCATCATAGTTGGAAGTTCTTAATACAAGTTGTCAATCACTGCCCTAAGCTTCAAAAACTTGACCTCAATGAGATCAAGCAGGATGAGGATGAAGAATTCTGGACTAAAAACATCGACAAAGAACATTGGGTGGACCCAAAATTTGTTCCGCAATGCCTTTCATTACACCTAAGAACTTGCAATTTTTTTAGTTTCTCAAGCCGACGGGGTCAGATTATGCTAGCAAGGTACACTACTAGAATCTCTCGTTTTTCCTGCGGATTTTCTTGCGGAAATATATCCGCAGGAACACCTGAGGATTTTGCTGCGGCTGCGATTAGTTAA

Protein sequence

>MsG0780037571.01.T01

LLSRDPALPINTFHLDVTYPYDLDLILDPHGPVSTFTKWVNFVVQRRVEYLYLSVVSSGLQYSKLPISVITCSTVVVLKLNRFIIQGGFSPVLLPSLKTLHLEDSSFPKLRDLMLLLTGCPILEDLFTFNVVFDSDESLTCDEWKIFCLSNLTYADIDCYRFHFPLEAVHNVPTLRLEIDKGYCRNDSIPTFHNLTHLVFICASLHHSWKFLIQVVNHCPKLQKLDLNEIKQDEDEEFWTKNIDKEHWVDPKFVPQCLSLHLRTCNFFSFSSRRGQIMLARYTTRISRFSCGFSCGNISAGTPEDFAAAAIS*