AlfalfaGEDB | MsG0280011333.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011333.01

KEGG: -
GO: GO:0005515
PFAM: PF01535 PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011333.01.T01	XP_003597780.1	82.593	540	20	4	45	510	1	540	0	880
MsG0280011333.01.T01	XP_003597780.1	27.696	408	268	6	112	496	230	633	1.52E-30	137

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011333.01.T01	Q9LW84	66.886	456	132	2	74	510	87	542	0	642

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011333.01.T01	G7II50	82.593	540	20	4	45	510	1	540	0.0	880

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048065.01	MsG0280011333.01	0.808606	2.851830e-50	1.637698e-47
MsG0080048307.01	MsG0280011333.01	0.801700	7.955502e-49	3.824369e-46
MsG0080048464.01	MsG0280011333.01	0.802029	6.808406e-49	3.300516e-46
MsG0080048878.01	MsG0280011333.01	0.800446	1.435741e-48	6.686533e-46
MsG0180000218.01	MsG0280011333.01	-0.838667	2.509507e-57	3.384844e-54
MsG0180000355.01	MsG0280011333.01	0.818886	1.550986e-52	1.175494e-49
MsG0180001002.01	MsG0280011333.01	0.814299	1.653448e-51	1.105330e-48
MsG0180001100.01	MsG0280011333.01	0.802809	4.702861e-49	2.325762e-46
MsG0180001105.01	MsG0280011333.01	0.804921	1.712481e-49	8.941179e-47
MsG0180001311.01	MsG0280011333.01	0.810301	1.233829e-50	7.410166e-48

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PPI

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Gene1	Gene2	Type
MsG0280011333.01	MsG0780041604.01	PPI
MsG0780041506.01	MsG0280011333.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011333.01.T01	MTR_2g102210	82.593	540	20	4	45	510	1	540	0.0	880
MsG0280011333.01.T01	MTR_2g102210	27.696	408	268	6	112	496	230	633	1.84e-34	137
MsG0280011333.01.T01	MTR_5g095130	26.850	473	290	7	92	511	112	581	1.17e-51	189
MsG0280011333.01.T01	MTR_5g095130	30.295	373	241	3	154	509	384	754	1.45e-49	183
MsG0280011333.01.T01	MTR_5g095130	27.902	491	323	8	40	509	344	824	6.79e-44	166
MsG0280011333.01.T01	MTR_5g095130	27.273	385	262	2	144	511	478	861	2.39e-39	153
MsG0280011333.01.T01	MTR_5g095130	26.291	426	293	3	112	517	481	905	5.22e-39	152
MsG0280011333.01.T01	MTR_5g095130	24.474	380	265	5	154	514	103	479	1.62e-30	126
MsG0280011333.01.T01	MTR_5g090170	28.635	447	287	9	85	508	66	503	4.64e-50	182
MsG0280011333.01.T01	MTR_5g090170	25.877	456	287	6	112	517	234	688	1.01e-38	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011333.01.T01	AT3G16010	66.886	456	132	2	74	510	87	542	0.0	642
MsG0280011333.01.T01	AT3G16010	25.994	327	223	3	115	423	305	630	4.05e-30	125
MsG0280011333.01.T01	AT3G16010	25.279	269	181	3	115	364	375	642	2.19e-18	89.0
MsG0280011333.01.T01	AT3G06920	30.214	374	242	3	154	510	349	720	3.25e-50	185
MsG0280011333.01.T01	AT3G06920	25.152	493	311	6	73	511	58	546	8.89e-47	175
MsG0280011333.01.T01	AT3G06920	28.198	383	255	3	148	511	445	826	8.83e-42	160
MsG0280011333.01.T01	AT3G06920	27.596	366	246	2	112	477	481	827	4.10e-40	155
MsG0280011333.01.T01	AT3G06920	26.747	415	250	5	155	517	245	657	6.11e-39	152
MsG0280011333.01.T01	AT3G06920	30.214	374	242	3	154	510	363	734	3.51e-50	185
MsG0280011333.01.T01	AT3G06920	24.900	498	316	6	68	511	67	560	9.01e-47	175

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Find 110 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGCTTTACTGCTTCTTG+AGG	0.580153	2:+82527615	MsG0280011333.01.T01:CDS
AAATTGTGAAAATCTTGGGT+AGG	0.544723	2:+82526838	MsG0280011333.01.T01:CDS
AACCATGAAGAAGCCTCAGA+AGG	0.602790	2:-82527505	None:intergenic
AAGGATTTAAGCTATGAAAT+TGG	0.280190	2:-82525764	None:intergenic
AATAAAGAGTGGATTTGAAT+TGG	0.312051	2:-82525721	None:intergenic
AATCTTGGGTAGGGTTAAGA+TGG	0.458908	2:+82526848	MsG0280011333.01.T01:CDS
AATGAGTATATACTTTAAGT+TGG	0.406232	2:+82527159	MsG0280011333.01.T01:CDS
AATGCGGCGGTGCGTCCCAA+CGG	0.567234	2:+82527222	MsG0280011333.01.T01:CDS
AATTGAATATGTAAATGAGC+TGG	0.514452	2:-82527557	None:intergenic
AATTGTGAAAATCTTGGGTA+GGG	0.538084	2:+82526839	MsG0280011333.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTGTGAAAATCTTGGGT+AGG	+	Chr2:82526838-82526857	MsG0280011333.01.T01:CDS	30.0%
AATAGGTTCTTTGTTAACGA+AGG	+	Chr2:82526327-82526346	MsG0280011333.01.T01:intron	30.0%
AATTGGATGAAAGGTTCATT+AGG	+	Chr2:82526527-82526546	MsG0280011333.01.T01:intron	30.0%
AATTGTGAAAATCTTGGGTA+GGG	+	Chr2:82526839-82526858	MsG0280011333.01.T01:CDS	30.0%
ACAATTTCAGACAATTCAGA+AGG	-	Chr2:82526825-82526844	None:intergenic	30.0%
ACATATTCAATTCTCATCGA+TGG	+	Chr2:82527568-82527587	MsG0280011333.01.T01:CDS	30.0%
ACATGATTCGACAACTTATA+TGG	+	Chr2:82526710-82526729	MsG0280011333.01.T01:CDS	30.0%
ACCCTTAACCTGATAAAAAA+TGG	-	Chr2:82526889-82526908	None:intergenic	30.0%
AGTGAGAGTAAAATAAAGAG+TGG	-	Chr2:82525735-82525754	None:intergenic	30.0%
ATAGGTTCTTTGTTAACGAA+GGG	+	Chr2:82526328-82526347	MsG0280011333.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	82525634	82527987	82525634	ID=MsG0280011333.01;Name=MsG0280011333.01
Chr2	mRNA	82525634	82527987	82525634	ID=MsG0280011333.01.T01;Parent=MsG0280011333.01;Name=MsG0280011333.01.T01;_AED=0.41;_eAED=0.41;_QI=35\|0\|0.33\|1\|0.5\|0.33\|3\|0\|517
Chr2	exon	82525634	82525876	82525634	ID=MsG0280011333.01.T01:exon:2941;Parent=MsG0280011333.01.T01
Chr2	exon	82526589	82527008	82526589	ID=MsG0280011333.01.T01:exon:2942;Parent=MsG0280011333.01.T01
Chr2	exon	82527062	82527987	82527062	ID=MsG0280011333.01.T01:exon:2943;Parent=MsG0280011333.01.T01
Chr2	five_prime_UTR	82525634	82525668	82525634	ID=MsG0280011333.01.T01:five_prime_utr;Parent=MsG0280011333.01.T01
Chr2	CDS	82525669	82525876	82525669	ID=MsG0280011333.01.T01:cds;Parent=MsG0280011333.01.T01
Chr2	CDS	82526589	82527008	82526589	ID=MsG0280011333.01.T01:cds;Parent=MsG0280011333.01.T01
Chr2	CDS	82527062	82527987	82527062	ID=MsG0280011333.01.T01:cds;Parent=MsG0280011333.01.T01

Gene Sequence

>MsG0280011333.01.T01

ATGGAACAATGTCAACGGTTTTTGACGCATACACAAACTACGAAATACGAATCCAATTCAAATCCACTCTTTATTTTACTCTCACTTTCTCGGAACCAATTTCATAGCTTAAATCCTTATTGGGTATCAACAATGCTCCCTGGATTCATTACTGCCAGGAGATTCATTTCTACTTCGCTTCCTTTCTCTCAGAGATTAAAACAAACAGGCTTTGGAAGTATGAAGCTTAAGCTTGATATTCGCTTAGTTCGCGAAGTTTTGAAGATAGATGTTGAGGTCCATGTGAAGATTCAGTTTTTTAAGTGGGCTGGGAAGAAAAGGAATTTTGAACATGATTCGACAACTTATATGGCTTTGATACGATGCTTAGATGAAAATAGGTTGGTTGGTGAACTGTGGAGGACAATACAAGACATGGTTAAGAGTCCATGTGCGATTGGTCCTTCTGAATTGTCTGAAATTGTGAAAATCTTGGGTAGGGTTAAGATGGTTAACAAGGCATTATCCATTTTTTATCAGGTTAAGGGTCGCAAGTGCAGACCTACAGCAGGCACTTACAACTCTGTCATCTTGATGTTGATGCAAGAGGGGCATCATGAAAAAGTTCATGAGTTGTATAATGAAATGTCGTTTGGAAAACTAAATCGCGATGATTCTGCCGTCAGATTATTTAATGAGATGAAGGAGAATGGATTACAGCCAACAGCAAAAATTTATACAACTTTAATGAGTATATACTTTAAGTTGGGTAAGGTTGAGGAAGCGTTGAACCTGGTCCACGAGATGAGAATGCGGCGGTGCGTCCCAACGGTCTATACTTATACAGAATTGATAAGGGGATTGGGGAAATCCGGGAGGGTTGAAGACGCATGCGGAGTATACAAGAATATGCTGAAAGATGGTTGCAAACCAGATGTTGTCCTCATGAATAATTTGATTAACATTTTGGGTAGATCAGATCGCTTAAAAGAGGCTGTTGAGCTTTTTGAGGAAATGAATTTGCTTAATTGCACACCTAATGTTGTGACATACAACACCATTATCAAATCTCTATTTGAGGCTAAAGCCCCACCTTCTGAGGCTTCTTCATGGTTAGAGAGAATGAAAAAAGATGGAGTAGTTCCCAGCTCATTTACATATTCAATTCTCATCGATGGTTTCTGCAAAACAAATCGGGTTGAAAAGGCTTTACTGCTTCTTGAGGAGATGGATGAAAAAGGCTTTCCGCCTTGTCCTGCTGCCTACTGCAGCCTGATCAATAGCCTCGGTAAAGCAAAACGTTATGAGGCTGCAAATGAGCTGTTCCAAGAATTGAAAGAGAACTGTGGAAGTTCGAGCGCGCGTGTATATGCTGTCATGATTAAACATTTTGGAAAATGTGGACGATTCAATGAAGCTATGGGTCTTTTCAACGAGATGAAAAAACTTGGATGCATCCCCGATGTTTATGCTTATAATGCTCTCATGACCGGGATGGTAAGGGAAGACATGATGGATGAAGCTTTTTCTTTGTTTCGAACTATGAAGAAGAAAATGGTTGCAACCCAGATATAA

Protein sequence

>MsG0280011333.01.T01

MEQCQRFLTHTQTTKYESNSNPLFILLSLSRNQFHSLNPYWVSTMLPGFITARRFISTSLPFSQRLKQTGFGSMKLKLDIRLVREVLKIDVEVHVKIQFFKWAGKKRNFEHDSTTYMALIRCLDENRLVGELWRTIQDMVKSPCAIGPSELSEIVKILGRVKMVNKALSIFYQVKGRKCRPTAGTYNSVILMLMQEGHHEKVHELYNEMSFGKLNRDDSAVRLFNEMKENGLQPTAKIYTTLMSIYFKLGKVEEALNLVHEMRMRRCVPTVYTYTELIRGLGKSGRVEDACGVYKNMLKDGCKPDVVLMNNLINILGRSDRLKEAVELFEEMNLLNCTPNVVTYNTIIKSLFEAKAPPSEASSWLERMKKDGVVPSSFTYSILIDGFCKTNRVEKALLLLEEMDEKGFPPCPAAYCSLINSLGKAKRYEAANELFQELKENCGSSSARVYAVMIKHFGKCGRFNEAMGLFNEMKKLGCIPDVYAYNALMTGMVREDMMDEAFSLFRTMKKKMVATQI*