Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006931.01.T01 | XP_024631558.1 | 94.286 | 175 | 10 | 0 | 1 | 175 | 57 | 231 | 1.19E-118 | 345 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006931.01.T01 | A0A396J6F1 | 94.286 | 175 | 10 | 0 | 1 | 175 | 57 | 231 | 5.70e-119 | 345 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000114.01 | MsG0280006931.01 | 0.826364 | 2.824541e-54 | 2.643378e-51 |
| MsG0180000363.01 | MsG0280006931.01 | 0.803764 | 2.982708e-49 | 1.511520e-46 |
| MsG0180000611.01 | MsG0280006931.01 | 0.801701 | 7.954200e-49 | 3.823773e-46 |
| MsG0180000680.01 | MsG0280006931.01 | 0.804314 | 2.293086e-49 | 1.178548e-46 |
| MsG0180002547.01 | MsG0280006931.01 | 0.803940 | 2.742595e-49 | 1.396209e-46 |
| MsG0180002955.01 | MsG0280006931.01 | 0.814877 | 1.231351e-51 | 8.360406e-49 |
| MsG0180003357.01 | MsG0280006931.01 | 0.804723 | 1.884223e-49 | 9.787408e-47 |
| MsG0180003510.01 | MsG0280006931.01 | 0.870150 | 1.813482e-66 | 6.981534e-63 |
| MsG0180005106.01 | MsG0280006931.01 | 0.806846 | 6.746869e-50 | 3.700623e-47 |
| MsG0180005160.01 | MsG0280006931.01 | 0.816900 | 4.356958e-52 | 3.126500e-49 |
| MsG0180005268.01 | MsG0280006931.01 | 0.801616 | 8.278383e-49 | 3.971101e-46 |
| MsG0180005730.01 | MsG0280006931.01 | 0.807059 | 6.080989e-50 | 3.353799e-47 |
| MsG0180005786.01 | MsG0280006931.01 | 0.800726 | 1.258902e-48 | 5.904699e-46 |
| MsG0180006155.01 | MsG0280006931.01 | 0.810216 | 1.287027e-50 | 7.712447e-48 |
| MsG0280006634.01 | MsG0280006931.01 | 0.801827 | 7.493537e-49 | 3.614054e-46 |
| MsG0280006669.01 | MsG0280006931.01 | 0.801837 | 7.457843e-49 | 3.597796e-46 |
| MsG0280006931.01 | MsG0280007585.01 | 0.824530 | 7.676933e-54 | 6.818323e-51 |
| MsG0280006931.01 | MsG0280007775.01 | 0.803127 | 4.043916e-49 | 2.015900e-46 |
| MsG0280006931.01 | MsG0280008113.01 | 0.813599 | 2.359180e-51 | 1.547424e-48 |
| MsG0280006931.01 | MsG0280008765.01 | 0.809240 | 2.086262e-50 | 1.218531e-47 |
| MsG0280006931.01 | MsG0280008932.01 | 0.801101 | 1.055389e-48 | 4.997823e-46 |
| MsG0280006931.01 | MsG0280009350.01 | 0.804584 | 2.014238e-49 | 1.042449e-46 |
| MsG0280006931.01 | MsG0280009441.01 | 0.805413 | 1.351053e-49 | 7.143245e-47 |
| MsG0280006931.01 | MsG0280009598.01 | 0.804412 | 2.187552e-49 | 1.127115e-46 |
| MsG0280006931.01 | MsG0280010560.01 | 0.800682 | 1.285303e-48 | 6.021721e-46 |
| MsG0280006931.01 | MsG0280010599.01 | 0.820372 | 7.102850e-53 | 5.610027e-50 |
| MsG0280006931.01 | MsG0280010648.01 | 0.821749 | 3.420969e-53 | 2.807562e-50 |
| MsG0280006931.01 | MsG0280011201.01 | 0.808149 | 3.568694e-50 | 2.025132e-47 |
| MsG0280006931.01 | MsG0280011371.01 | 0.822428 | 2.382129e-53 | 1.993366e-50 |
| MsG0280006931.01 | MsG0380011669.01 | 0.803223 | 3.862052e-49 | 1.930078e-46 |
| MsG0280006931.01 | MsG0380012184.01 | 0.800949 | 1.133857e-48 | 5.348604e-46 |
| MsG0280006931.01 | MsG0380013176.01 | 0.806403 | 8.366146e-50 | 4.536771e-47 |
| MsG0280006931.01 | MsG0380014866.01 | 0.813598 | 2.360690e-51 | 1.548366e-48 |
| MsG0280006931.01 | MsG0380015220.01 | 0.807349 | 5.278452e-50 | 2.933232e-47 |
| MsG0280006931.01 | MsG0380016133.01 | 0.832410 | 9.606399e-56 | 1.072871e-52 |
| MsG0280006931.01 | MsG0380017229.01 | 0.801895 | 7.256708e-49 | 3.505970e-46 |
| MsG0280006931.01 | MsG0480021959.01 | 0.803058 | 4.179104e-49 | 2.079607e-46 |
| MsG0280006931.01 | MsG0480021998.01 | 0.819526 | 1.108861e-52 | 8.554397e-50 |
| MsG0280006931.01 | MsG0480022054.01 | 0.800827 | 1.200522e-48 | 5.645331e-46 |
| MsG0280006931.01 | MsG0480022292.01 | 0.804749 | 1.860362e-49 | 9.670047e-47 |
| MsG0280006931.01 | MsG0480022343.01 | 0.809037 | 2.306269e-50 | 1.339634e-47 |
| MsG0280006931.01 | MsG0480022916.01 | 0.800669 | 1.292946e-48 | 6.055798e-46 |
| MsG0280006931.01 | MsG0480023554.01 | 0.800597 | 1.337377e-48 | 6.252345e-46 |
| MsG0280006931.01 | MsG0580024536.01 | 0.809489 | 1.845104e-50 | 1.084768e-47 |
| MsG0280006931.01 | MsG0580024829.01 | 0.829027 | 6.475651e-55 | 6.547533e-52 |
| MsG0280006931.01 | MsG0580025009.01 | 0.813531 | 2.441896e-51 | 1.598747e-48 |
| MsG0280006931.01 | MsG0580025986.01 | 0.811122 | 8.196749e-51 | 5.031726e-48 |
| MsG0280006931.01 | MsG0580026225.01 | 0.805584 | 1.243823e-49 | 6.605309e-47 |
| MsG0280006931.01 | MsG0580026664.01 | 0.816001 | 6.923996e-52 | 4.847996e-49 |
| MsG0280006931.01 | MsG0580027891.01 | 0.809593 | 1.752406e-50 | 1.033193e-47 |
| MsG0280006931.01 | MsG0580028918.01 | 0.821722 | 3.471521e-53 | 2.846921e-50 |
| MsG0280006931.01 | MsG0580029794.01 | 0.814519 | 1.478193e-51 | 9.940828e-49 |
| MsG0280006931.01 | MsG0580030142.01 | 0.805373 | 1.377685e-49 | 7.276432e-47 |
| MsG0280006931.01 | MsG0680030415.01 | 0.804040 | 2.614746e-49 | 1.334476e-46 |
| MsG0280006931.01 | MsG0680031815.01 | 0.800308 | 1.531673e-48 | 7.108836e-46 |
| MsG0280006931.01 | MsG0780036117.01 | 0.813165 | 2.938736e-51 | 1.904749e-48 |
| MsG0280006931.01 | MsG0780039143.01 | 0.808742 | 2.667416e-50 | 1.537238e-47 |
| MsG0280006931.01 | MsG0780040254.01 | 0.815330 | 9.769208e-52 | 6.715563e-49 |
| MsG0280006931.01 | MsG0780040758.01 | 0.810774 | 9.751882e-51 | 5.931427e-48 |
| MsG0280006931.01 | MsG0780041210.01 | 0.836338 | 9.921945e-57 | 1.246760e-53 |
| MsG0280006931.01 | MsG0780041498.01 | 0.811191 | 7.922495e-51 | 4.872117e-48 |
| MsG0280006931.01 | MsG0880042223.01 | 0.801155 | 1.028755e-48 | 4.878188e-46 |
| MsG0280006931.01 | MsG0880042622.01 | 0.802614 | 5.161324e-49 | 2.539600e-46 |
| MsG0280006931.01 | MsG0880042743.01 | 0.802219 | 6.222832e-49 | 3.031250e-46 |
| MsG0280006931.01 | MsG0880043162.01 | 0.827955 | 1.175270e-54 | 1.151870e-51 |
| MsG0280006931.01 | MsG0880043804.01 | 0.802766 | 4.800978e-49 | 2.371599e-46 |
| MsG0280006931.01 | MsG0880045106.01 | 0.819624 | 1.053442e-52 | 8.148312e-50 |
| MsG0280006931.01 | MsG0880045777.01 | 0.807490 | 4.928999e-50 | 2.749122e-47 |
| MsG0280006931.01 | MsG0880046395.01 | 0.807495 | 4.916941e-50 | 2.742757e-47 |
| MsG0280006931.01 | MsG0880047252.01 | 0.826996 | 1.995906e-54 | 1.902141e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006931.01.T01 | MTR_2g016760 | 94.253 | 174 | 10 | 0 | 1 | 174 | 57 | 230 | 1.74e-120 | 345 |
| MsG0280006931.01.T01 | MTR_8g086820 | 60.440 | 182 | 63 | 3 | 1 | 175 | 63 | 242 | 5.78e-64 | 197 |
| MsG0280006931.01.T01 | MTR_2g083910 | 51.235 | 162 | 71 | 2 | 14 | 175 | 80 | 233 | 5.93e-49 | 158 |
| MsG0280006931.01.T01 | MTR_2g083910 | 50.943 | 159 | 70 | 2 | 14 | 172 | 80 | 230 | 4.93e-47 | 154 |
| MsG0280006931.01.T01 | MTR_4g080340 | 44.565 | 184 | 89 | 2 | 5 | 175 | 56 | 239 | 3.20e-39 | 134 |
| MsG0280006931.01.T01 | MTR_2g078620 | 46.364 | 110 | 47 | 4 | 66 | 173 | 221 | 320 | 1.78e-17 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006931.01.T01 | AT2G42760 | 48.603 | 179 | 70 | 8 | 18 | 175 | 89 | 266 | 1.51e-36 | 127 |
Find 34 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGAGTTTGAGGAGGTAAA+AGG | 0.237638 | 2:-8086017 | MsG0280006931.01.T01:CDS |
| AAGATAAACCTTTAATGAAA+TGG | 0.354709 | 2:-8085818 | MsG0280006931.01.T01:CDS |
| AGCTTGGTTTCAATCATTCC+TGG | 0.362803 | 2:-8085948 | MsG0280006931.01.T01:CDS |
| TTTCTTGTGACGTTCTTGTT+AGG | 0.362919 | 2:+8086077 | None:intergenic |
| AACAAGAACGTCACAAGAAA+TGG | 0.373690 | 2:-8086074 | MsG0280006931.01.T01:CDS |
| AACTTGTCATGGTGTTTAAC+TGG | 0.386459 | 2:+8086231 | None:intergenic |
| TTTATCAGACCTTGAGTTTG+AGG | 0.393633 | 2:-8086027 | MsG0280006931.01.T01:CDS |
| GTAAAAGGGTTCATGGATCT+TGG | 0.397551 | 2:-8086002 | MsG0280006931.01.T01:CDS |
| TTCATGTCAACTTCATTAGT+AGG | 0.399570 | 2:+8085783 | None:intergenic |
| TGAGGAGGTAAAAGGGTTCA+TGG | 0.400518 | 2:-8086009 | MsG0280006931.01.T01:CDS |
| GGTTGGACAAGGACAGAGTT+TGG | 0.405732 | 2:+8086188 | None:intergenic |
| ATTCCTGGTTTGCAAAGGTT+AGG | 0.414865 | 2:-8085933 | MsG0280006931.01.T01:CDS |
| TTCCCTAACCTTTGCAAACC+AGG | 0.424502 | 2:+8085930 | None:intergenic |
| TTCCTGGTTTGCAAAGGTTA+GGG | 0.442705 | 2:-8085932 | MsG0280006931.01.T01:CDS |
| GAAATATAGTCTCAAATGTT+GGG | 0.446438 | 2:-8085763 | MsG0280006931.01.T01:CDS |
| GACCTTACCTTTCTGAAGCA+TGG | 0.450117 | 2:-8085863 | MsG0280006931.01.T01:CDS |
| TGAAATATAGTCTCAAATGT+TGG | 0.461940 | 2:-8085764 | MsG0280006931.01.T01:CDS |
| ACCTTACCTTTCTGAAGCAT+GGG | 0.502044 | 2:-8085862 | MsG0280006931.01.T01:CDS |
| TCAACTTCATTAGTAGGAGC+AGG | 0.522419 | 2:+8085789 | None:intergenic |
| GGTGTTTAACTGGTCACTCA+TGG | 0.527991 | 2:+8086241 | None:intergenic |
| TTGAGTTTGAGGAGGTAAAA+GGG | 0.532051 | 2:-8086016 | MsG0280006931.01.T01:CDS |
| GAGAGGATTGTTTGAAGCTG+TGG | 0.532154 | 2:+8086167 | None:intergenic |
| AGAAATGGAAGAAGGAAAAG+TGG | 0.534698 | 2:-8086059 | MsG0280006931.01.T01:CDS |
| CAGCTTCAAACAATCCTCTC+AGG | 0.536712 | 2:-8086164 | MsG0280006931.01.T01:CDS |
| CAATCATTCCTGGTTTGCAA+AGG | 0.550217 | 2:-8085938 | MsG0280006931.01.T01:CDS |
| GGAAGATAAAGACACAAGCT+TGG | 0.560492 | 2:-8085964 | MsG0280006931.01.T01:CDS |
| GGATTGTTTGAAGCTGTGGT+TGG | 0.563182 | 2:+8086171 | None:intergenic |
| ACGTCACAAGAAATGGAAGA+AGG | 0.567190 | 2:-8086067 | MsG0280006931.01.T01:CDS |
| TCCCATGCTTCAGAAAGGTA+AGG | 0.575358 | 2:+8085861 | None:intergenic |
| GATCCTCCCATGCTTCAGAA+AGG | 0.586388 | 2:+8085856 | None:intergenic |
| TTACCTTTCTGAAGCATGGG+AGG | 0.592142 | 2:-8085859 | MsG0280006931.01.T01:CDS |
| TTTGAAGCTGTGGTTGGACA+AGG | 0.594695 | 2:+8086177 | None:intergenic |
| ATCAGACCTTGAGTTTGAGG+AGG | 0.598693 | 2:-8086024 | MsG0280006931.01.T01:CDS |
| ATCATGTTTGAAACTTGTCA+TGG | 0.648612 | 2:+8086220 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGATAAACCTTTAATGAAA+TGG | - | Chr2:8086155-8086174 | MsG0280006931.01.T01:CDS | 20.0% |
| ! | GAAATATAGTCTCAAATGTT+GGG | - | Chr2:8086210-8086229 | MsG0280006931.01.T01:CDS | 25.0% |
| ! | TGAAATATAGTCTCAAATGT+TGG | - | Chr2:8086209-8086228 | MsG0280006931.01.T01:CDS | 25.0% |
| !! | GGAATTTTCCATTTCATTAA+AGG | + | Chr2:8086166-8086185 | None:intergenic | 25.0% |
| TTCATGTCAACTTCATTAGT+AGG | + | Chr2:8086193-8086212 | None:intergenic | 30.0% | |
| ! | ATCATGTTTGAAACTTGTCA+TGG | + | Chr2:8085756-8085775 | None:intergenic | 30.0% |
| AACAAGAACGTCACAAGAAA+TGG | - | Chr2:8085899-8085918 | MsG0280006931.01.T01:CDS | 35.0% | |
| AACTTGTCATGGTGTTTAAC+TGG | + | Chr2:8085745-8085764 | None:intergenic | 35.0% | |
| AGAAATGGAAGAAGGAAAAG+TGG | - | Chr2:8085914-8085933 | MsG0280006931.01.T01:CDS | 35.0% | |
| TTGAGTTTGAGGAGGTAAAA+GGG | - | Chr2:8085957-8085976 | MsG0280006931.01.T01:CDS | 35.0% | |
| TTGCAAAGGTTAGGGAAAAA+TGG | - | Chr2:8086049-8086068 | MsG0280006931.01.T01:CDS | 35.0% | |
| TTTATCAGACCTTGAGTTTG+AGG | - | Chr2:8085946-8085965 | MsG0280006931.01.T01:CDS | 35.0% | |
| TTTCTTGTGACGTTCTTGTT+AGG | + | Chr2:8085899-8085918 | None:intergenic | 35.0% | |
| !!! | TCTTGGTTTTGTTTTCTCAG+AGG | - | Chr2:8085988-8086007 | MsG0280006931.01.T01:CDS | 35.0% |
| ACGTCACAAGAAATGGAAGA+AGG | - | Chr2:8085906-8085925 | MsG0280006931.01.T01:CDS | 40.0% | |
| AGCTTGGTTTCAATCATTCC+TGG | - | Chr2:8086025-8086044 | MsG0280006931.01.T01:CDS | 40.0% | |
| CTTGAGTTTGAGGAGGTAAA+AGG | - | Chr2:8085956-8085975 | MsG0280006931.01.T01:CDS | 40.0% | |
| GGAAGATAAAGACACAAGCT+TGG | - | Chr2:8086009-8086028 | MsG0280006931.01.T01:CDS | 40.0% | |
| GTAAAAGGGTTCATGGATCT+TGG | - | Chr2:8085971-8085990 | MsG0280006931.01.T01:CDS | 40.0% | |
| TCAACTTCATTAGTAGGAGC+AGG | + | Chr2:8086187-8086206 | None:intergenic | 40.0% | |
| ! | ATTCCTGGTTTGCAAAGGTT+AGG | - | Chr2:8086040-8086059 | MsG0280006931.01.T01:CDS | 40.0% |
| ! | CAATCATTCCTGGTTTGCAA+AGG | - | Chr2:8086035-8086054 | MsG0280006931.01.T01:CDS | 40.0% |
| ! | CTTTTACCTCCTCAAACTCA+AGG | + | Chr2:8085958-8085977 | None:intergenic | 40.0% |
| ! | TTCCTGGTTTGCAAAGGTTA+GGG | - | Chr2:8086041-8086060 | MsG0280006931.01.T01:CDS | 40.0% |
| !! | ACCTTACCTTTCTGAAGCAT+GGG | - | Chr2:8086111-8086130 | MsG0280006931.01.T01:CDS | 40.0% |
| ATCAGACCTTGAGTTTGAGG+AGG | - | Chr2:8085949-8085968 | MsG0280006931.01.T01:CDS | 45.0% | |
| CAGCTTCAAACAATCCTCTC+AGG | - | Chr2:8085809-8085828 | MsG0280006931.01.T01:CDS | 45.0% | |
| TCCCATGCTTCAGAAAGGTA+AGG | + | Chr2:8086115-8086134 | None:intergenic | 45.0% | |
| TGAGGAGGTAAAAGGGTTCA+TGG | - | Chr2:8085964-8085983 | MsG0280006931.01.T01:CDS | 45.0% | |
| TTCCCTAACCTTTGCAAACC+AGG | + | Chr2:8086046-8086065 | None:intergenic | 45.0% | |
| ! | CTGTGACATCTTTTCCTGAG+AGG | + | Chr2:8085826-8085845 | None:intergenic | 45.0% |
| ! | GAGAGGATTGTTTGAAGCTG+TGG | + | Chr2:8085809-8085828 | None:intergenic | 45.0% |
| ! | GGATTGTTTGAAGCTGTGGT+TGG | + | Chr2:8085805-8085824 | None:intergenic | 45.0% |
| ! | TTTGAAGCTGTGGTTGGACA+AGG | + | Chr2:8085799-8085818 | None:intergenic | 45.0% |
| !! | GACCTTACCTTTCTGAAGCA+TGG | - | Chr2:8086110-8086129 | MsG0280006931.01.T01:CDS | 45.0% |
| !! | TTACCTTTCTGAAGCATGGG+AGG | - | Chr2:8086114-8086133 | MsG0280006931.01.T01:CDS | 45.0% |
| GATCCTCCCATGCTTCAGAA+AGG | + | Chr2:8086120-8086139 | None:intergenic | 50.0% | |
| GGTTGGACAAGGACAGAGTT+TGG | + | Chr2:8085788-8085807 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 8085734 | 8086261 | 8085734 | ID=MsG0280006931.01;Name=MsG0280006931.01 |
| Chr2 | mRNA | 8085734 | 8086261 | 8085734 | ID=MsG0280006931.01.T01;Parent=MsG0280006931.01;Name=MsG0280006931.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|175 |
| Chr2 | exon | 8085734 | 8086261 | 8085734 | ID=MsG0280006931.01.T01:exon:2369;Parent=MsG0280006931.01.T01 |
| Chr2 | CDS | 8085734 | 8086261 | 8085734 | ID=MsG0280006931.01.T01:cds;Parent=MsG0280006931.01.T01 |
| Gene Sequence |
| Protein sequence |
