AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027891.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027891.01.T01 MTR_5g064850 80.899 89 12 1 1 89 1 84 1.79e-18 78.2
MsG0580027891.01.T01 MTR_5g064850 80.899 89 12 1 1 89 1 84 1.97e-18 78.2
MsG0580027891.01.T02 MTR_5g064850 80.899 89 12 1 1 89 1 84 1.79e-18 78.2
MsG0580027891.01.T02 MTR_5g064850 80.899 89 12 1 1 89 1 84 1.97e-18 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGCTACCTAGGGTTTAGA+AGG 0.362705 5:-70493308 None:intergenic
GGTTATCTGAATTGTATAAC+AGG 0.416266 5:-70493274 None:intergenic
GGCTGCAATGGGAAGGGAAA+CGG 0.430404 5:-70493246 None:intergenic
AACAGGAGCGTGGCTGCAAT+GGG 0.468019 5:-70493257 None:intergenic
AAGAATTGTTACCTCGTTGA+AGG 0.468463 5:-70493519 None:intergenic
TAACAGGAGCGTGGCTGCAA+TGG 0.475171 5:-70493258 None:intergenic
GGAGCGTGGCTGCAATGGGA+AGG 0.532851 5:-70493253 None:intergenic
TGAACGTTGGAACGCTACCT+AGG 0.547135 5:-70493318 None:intergenic
GAGCGTGGCTGCAATGGGAA+GGG 0.555935 5:-70493252 None:intergenic
CGCTACCTAGGGTTTAGAAG+GGG 0.602023 5:-70493306 None:intergenic
GAACGTTGGAACGCTACCTA+GGG 0.609630 5:-70493317 None:intergenic
ACGCTACCTAGGGTTTAGAA+GGG 0.636366 5:-70493307 None:intergenic
GCTACCTAGGGTTTAGAAGG+GGG 0.656182 5:-70493305 None:intergenic
TGATATCACAGTGTGAACGT+TGG 0.682951 5:-70493331 None:intergenic
TGAATTGTATAACAGGAGCG+TGG 0.704680 5:-70493267 None:intergenic
TGATGATATGTCCTTCAACG+AGG 0.737794 5:+70493508 MsG0580027891.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAGTTTAAGTTTAAAAAT+GGG + Chr5:70493422-70493441 MsG0580027891.01.T01:exon 15.0%
!!! TTTGAGTTTAAGTTTAAAAA+TGG + Chr5:70493421-70493440 MsG0580027891.01.T01:exon 15.0%
!!! TTTTTTTTTCGATTCTTAAC+AGG + Chr5:70493361-70493380 MsG0580027891.01.T01:intron 20.0%
! TAAGTTTAAAAATGGGTAGT+AGG + Chr5:70493429-70493448 MsG0580027891.01.T01:exon 25.0%
!! TTTGTTGTGAATTCTGATTA+TGG + Chr5:70493588-70493607 MsG0580027891.01.T01:intron 25.0%
GGTTATCTGAATTGTATAAC+AGG - Chr5:70493277-70493296 None:intergenic 30.0%
! ACAACTTGAGAAAGAGTATA+TGG + Chr5:70493760-70493779 MsG0580027891.01.T01:CDS 30.0%
!! GATGATTTTTGTGATGATGT+AGG + Chr5:70493610-70493629 MsG0580027891.01.T01:intron 30.0%
AAGAATTGTTACCTCGTTGA+AGG - Chr5:70493522-70493541 None:intergenic 35.0%
GCAAAAAAGTGACAGTGATT+TGG + Chr5:70493475-70493494 MsG0580027891.01.T01:CDS 35.0%
TGGATGGAAAGATGATGAAA+TGG + Chr5:70493736-70493755 MsG0580027891.01.T01:CDS 35.0%
! GTAGGTTAATGATGATAGTG+AGG + Chr5:70493628-70493647 MsG0580027891.01.T01:intron 35.0%
AGAAGAAGTTGAGGGACAAA+AGG + Chr5:70493709-70493728 MsG0580027891.01.T01:CDS 40.0%
GATGAGGAAGAAGAAGTTGA+GGG + Chr5:70493701-70493720 MsG0580027891.01.T01:CDS 40.0%
GTATATGGATCTTCATCACC+AGG + Chr5:70493775-70493794 MsG0580027891.01.T01:CDS 40.0%
GTTTAGAAGGGGGAAAAAAG+CGG - Chr5:70493298-70493317 None:intergenic 40.0%
TGAATTGTATAACAGGAGCG+TGG - Chr5:70493270-70493289 None:intergenic 40.0%
TGATATCACAGTGTGAACGT+TGG - Chr5:70493334-70493353 None:intergenic 40.0%
TGATGATATGTCCTTCAACG+AGG + Chr5:70493508-70493527 MsG0580027891.01.T01:CDS 40.0%
! GGTTAATGATGATAGTGAGG+TGG + Chr5:70493631-70493650 MsG0580027891.01.T01:intron 40.0%
! TAATGATGATAGTGAGGTGG+AGG + Chr5:70493634-70493653 MsG0580027891.01.T01:CDS 40.0%
AACGCTACCTAGGGTTTAGA+AGG - Chr5:70493311-70493330 None:intergenic 45.0%
ACGCTACCTAGGGTTTAGAA+GGG - Chr5:70493310-70493329 None:intergenic 45.0%
AGGGACAAAAGGAAAGTGGA+TGG + Chr5:70493720-70493739 MsG0580027891.01.T01:CDS 45.0%
CGATGAGGAAGAAGAAGTTG+AGG + Chr5:70493700-70493719 MsG0580027891.01.T01:CDS 45.0%
GAGGAAGAGGAAGAAGATGA+TGG + Chr5:70493662-70493681 MsG0580027891.01.T01:CDS 45.0%
GTTGAGGGACAAAAGGAAAG+TGG + Chr5:70493716-70493735 MsG0580027891.01.T01:CDS 45.0%
! TTTTCCCCCTTCTAAACCCT+AGG + Chr5:70493301-70493320 MsG0580027891.01.T01:five_prime_UTR 45.0%
!! AGAAGATGATGGAGAGGATG+AGG + Chr5:70493673-70493692 MsG0580027891.01.T01:CDS 45.0%
!! AGAGGAAGAAGATGATGGAG+AGG + Chr5:70493667-70493686 MsG0580027891.01.T01:CDS 45.0%
!! GAAGATGATGGAGAGGATGA+GGG + Chr5:70493674-70493693 MsG0580027891.01.T01:CDS 45.0%
!! AAATAAATAACAAAAAAAAT+TGG - Chr5:70493553-70493572 None:intergenic 5.0%
CGCTACCTAGGGTTTAGAAG+GGG - Chr5:70493309-70493328 None:intergenic 50.0%
GAACGTTGGAACGCTACCTA+GGG - Chr5:70493320-70493339 None:intergenic 50.0%
GCTACCTAGGGTTTAGAAGG+GGG - Chr5:70493308-70493327 None:intergenic 50.0%
TGAACGTTGGAACGCTACCT+AGG - Chr5:70493321-70493340 None:intergenic 50.0%
! TGATGATAGTGAGGTGGAGG+AGG + Chr5:70493637-70493656 MsG0580027891.01.T01:CDS 50.0%
AGAGGATGAGGGAAGCGATG+AGG + Chr5:70493685-70493704 MsG0580027891.01.T01:CDS 55.0%
TAGTGAGGTGGAGGAGGAAG+AGG + Chr5:70493643-70493662 MsG0580027891.01.T01:CDS 55.0%
! AACAGGAGCGTGGCTGCAAT+GGG - Chr5:70493260-70493279 None:intergenic 55.0%
! TAACAGGAGCGTGGCTGCAA+TGG - Chr5:70493261-70493280 None:intergenic 55.0%
GAGCGTGGCTGCAATGGGAA+GGG - Chr5:70493255-70493274 None:intergenic 60.0%
GGTGGAGGAGGAAGAGGAAG+AGG + Chr5:70493649-70493668 MsG0580027891.01.T01:CDS 60.0%
GGAGCGTGGCTGCAATGGGA+AGG - Chr5:70493256-70493275 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 70493249 70493811 70493249 ID=MsG0580027891.01;Name=MsG0580027891.01
Chr5 mRNA 70493260 70493811 70493260 ID=MsG0580027891.01.T01;Parent=MsG0580027891.01;Name=MsG0580027891.01.T01;_AED=0.48;_eAED=0.48;_QI=144|1|0.66|1|0.5|0.33|3|0|89
Chr5 exon 70493260 70493371 70493260 ID=MsG0580027891.01.T01:exon:4777;Parent=MsG0580027891.01.T01
Chr5 exon 70493408 70493529 70493408 ID=MsG0580027891.01.T01:exon:4778;Parent=MsG0580027891.01.T01
Chr5 five_prime_UTR 70493260 70493371 70493260 ID=MsG0580027891.01.T01:five_prime_utr;Parent=MsG0580027891.01.T01
Chr5 five_prime_UTR 70493408 70493439 70493408 ID=MsG0580027891.01.T01:five_prime_utr;Parent=MsG0580027891.01.T01
Chr5 CDS 70493440 70493529 70493440 ID=MsG0580027891.01.T01:cds;Parent=MsG0580027891.01.T01
Chr5 CDS 70493632 70493811 70493632 ID=MsG0580027891.01.T01:cds;Parent=MsG0580027891.01.T01
Chr5 mRNA 70493249 70493811 70493249 ID=MsG0580027891.01.T02;Parent=MsG0580027891.01;Name=MsG0580027891.01.T02;_AED=0.48;_eAED=0.48;_QI=131|1|0.66|1|0.5|0.33|3|0|89
Chr5 exon 70493249 70493322 70493249 ID=MsG0580027891.01.T02:exon:4780;Parent=MsG0580027891.01.T02
Chr5 exon 70493383 70493529 70493383 ID=MsG0580027891.01.T02:exon:4781;Parent=MsG0580027891.01.T02
Chr5 five_prime_UTR 70493249 70493322 70493249 ID=MsG0580027891.01.T02:five_prime_utr;Parent=MsG0580027891.01.T02
Chr5 five_prime_UTR 70493383 70493439 70493383 ID=MsG0580027891.01.T02:five_prime_utr;Parent=MsG0580027891.01.T02
Chr5 CDS 70493440 70493529 70493440 ID=MsG0580027891.01.T02:cds;Parent=MsG0580027891.01.T02
Chr5 CDS 70493632 70493811 70493632 ID=MsG0580027891.01.T02:cds;Parent=MsG0580027891.01.T02
Gene Sequence

>MsG0580027891.01.T01

ATGGGTAGTAGGTCTGCAAAAAAATCTAAAAAAATGCAAAAAAGTGACAGTGATTTGGATGAATACGATGATGATATGTCCTTCAACGAGGTTAATGATGATAGTGAGGTGGAGGAGGAAGAGGAAGAGGAAGAAGATGATGGAGAGGATGAGGGAAGCGATGAGGAAGAAGAAGTTGAGGGACAAAAGGAAAGTGGATGGAAAGATGATGAAATGGAACAACTTGAGAAAGAGTATATGGATCTTCATCACCAGGAGCAAGAGCTGTGA

>MsG0580027891.01.T02

ATGGGTAGTAGGTCTGCAAAAAAATCTAAAAAAATGCAAAAAAGTGACAGTGATTTGGATGAATACGATGATGATATGTCCTTCAACGAGGTTAATGATGATAGTGAGGTGGAGGAGGAAGAGGAAGAGGAAGAAGATGATGGAGAGGATGAGGGAAGCGATGAGGAAGAAGAAGTTGAGGGACAAAAGGAAAGTGGATGGAAAGATGATGAAATGGAACAACTTGAGAAAGAGTATATGGATCTTCATCACCAGGAGCAAGAGCTGTGA

Protein sequence

>MsG0580027891.01.T01

MGSRSAKKSKKMQKSDSDLDEYDDDMSFNEVNDDSEVEEEEEEEEDDGEDEGSDEEEEVEGQKESGWKDDEMEQLEKEYMDLHHQEQEL*

>MsG0580027891.01.T02

MGSRSAKKSKKMQKSDSDLDEYDDDMSFNEVNDDSEVEEEEEEEEDDGEDEGSDEEEEVEGQKESGWKDDEMEQLEKEYMDLHHQEQEL*