Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012146.01.T01 | XP_003598516.1 | 81.707 | 246 | 30 | 3 | 1 | 241 | 94 | 329 | 1.02E-132 | 387 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012146.01.T01 | Q94CK9 | 47.236 | 199 | 100 | 5 | 41 | 236 | 112 | 308 | 2.12E-47 | 162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012146.01.T01 | G7J0K6 | 81.707 | 246 | 30 | 3 | 1 | 241 | 94 | 329 | 4.85e-133 | 387 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0380012146.01.T01 | TF | SRS |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048701.01 | MsG0380012146.01 | 0.800613 | 1.327592e-48 | 6.209152e-46 |
| MsG0080049140.01 | MsG0380012146.01 | 0.808769 | 2.631932e-50 | 1.517860e-47 |
| MsG0180000353.01 | MsG0380012146.01 | 0.867091 | 1.766494e-65 | 6.091452e-62 |
| MsG0180000360.01 | MsG0380012146.01 | 0.844429 | 7.600575e-59 | 1.226703e-55 |
| MsG0180000373.01 | MsG0380012146.01 | 0.851513 | 8.453981e-61 | 1.715228e-57 |
| MsG0180001565.01 | MsG0380012146.01 | 0.801380 | 9.251536e-49 | 4.411775e-46 |
| MsG0180003281.01 | MsG0380012146.01 | 0.816235 | 6.139502e-52 | 4.326260e-49 |
| MsG0180004320.01 | MsG0380012146.01 | 0.806090 | 9.736477e-50 | 5.238104e-47 |
| MsG0180004357.01 | MsG0380012146.01 | 0.819255 | 1.278272e-52 | 9.786854e-50 |
| MsG0180005388.01 | MsG0380012146.01 | -0.809778 | 1.599030e-50 | 9.474105e-48 |
| MsG0180005835.01 | MsG0380012146.01 | 0.806100 | 9.690989e-50 | 5.214837e-47 |
| MsG0180005921.01 | MsG0380012146.01 | 0.802823 | 4.671900e-49 | 2.311289e-46 |
| MsG0180006000.01 | MsG0380012146.01 | 0.810651 | 1.036631e-50 | 6.284415e-48 |
| MsG0180006260.01 | MsG0380012146.01 | 0.806513 | 7.932960e-50 | 4.314029e-47 |
| MsG0280006311.01 | MsG0380012146.01 | 0.828595 | 8.237366e-55 | 8.225019e-52 |
| MsG0280006420.01 | MsG0380012146.01 | 0.803849 | 2.864280e-49 | 1.454707e-46 |
| MsG0280006425.01 | MsG0380012146.01 | 0.806827 | 6.807805e-50 | 3.732215e-47 |
| MsG0280010779.01 | MsG0380012146.01 | 0.819653 | 1.037278e-52 | 8.030246e-50 |
| MsG0280010795.01 | MsG0380012146.01 | 0.812919 | 3.327935e-51 | 2.142830e-48 |
| MsG0280010868.01 | MsG0380012146.01 | 0.826023 | 3.405469e-54 | 3.156242e-51 |
| MsG0280011282.01 | MsG0380012146.01 | 0.854620 | 1.090520e-61 | 2.450164e-58 |
| MsG0380012146.01 | MsG0380012471.01 | 0.832954 | 7.038002e-56 | 7.988678e-53 |
| MsG0380012146.01 | MsG0380014754.01 | 0.833584 | 4.902770e-56 | 5.670852e-53 |
| MsG0380012146.01 | MsG0380015548.01 | 0.811063 | 8.442684e-51 | 5.174755e-48 |
| MsG0380012146.01 | MsG0380015677.01 | 0.808682 | 2.747455e-50 | 1.580885e-47 |
| MsG0380012146.01 | MsG0380016018.01 | 0.811267 | 7.627371e-51 | 4.699968e-48 |
| MsG0380012146.01 | MsG0380016140.01 | 0.805222 | 1.481155e-49 | 7.792652e-47 |
| MsG0380012146.01 | MsG0380016553.01 | 0.815000 | 1.156846e-51 | 7.881035e-49 |
| MsG0380012146.01 | MsG0380016703.01 | 0.815614 | 8.447631e-52 | 5.852926e-49 |
| MsG0380012146.01 | MsG0380016994.01 | 0.835067 | 2.081358e-56 | 2.516768e-53 |
| MsG0380012146.01 | MsG0380017049.01 | 0.811502 | 6.781938e-51 | 4.205292e-48 |
| MsG0380012146.01 | MsG0380017239.01 | 0.865234 | 6.845323e-65 | 2.211038e-61 |
| MsG0380012146.01 | MsG0480018397.01 | 0.819035 | 1.434504e-52 | 1.091705e-49 |
| MsG0380012146.01 | MsG0480018919.01 | 0.812521 | 4.065874e-51 | 2.590924e-48 |
| MsG0380012146.01 | MsG0480019635.01 | 0.845344 | 4.307145e-59 | 7.156268e-56 |
| MsG0380012146.01 | MsG0480020263.01 | 0.830431 | 2.948873e-55 | 3.106169e-52 |
| MsG0380012146.01 | MsG0480020965.01 | 0.802494 | 5.464171e-49 | 2.680332e-46 |
| MsG0380012146.01 | MsG0480021156.01 | 0.843875 | 1.070774e-58 | 1.698575e-55 |
| MsG0380012146.01 | MsG0480021350.01 | 0.813978 | 1.946846e-51 | 1.290059e-48 |
| MsG0380012146.01 | MsG0480022325.01 | 0.832970 | 6.973077e-56 | 7.919003e-53 |
| MsG0380012146.01 | MsG0480022331.01 | 0.809750 | 1.621189e-50 | 9.598398e-48 |
| MsG0380012146.01 | MsG0480022480.01 | 0.808516 | 2.980413e-50 | 1.707629e-47 |
| MsG0380012146.01 | MsG0480022866.01 | 0.800124 | 1.669424e-48 | 7.711680e-46 |
| MsG0380012146.01 | MsG0480023196.01 | 0.804322 | 2.283702e-49 | 1.173982e-46 |
| MsG0380012146.01 | MsG0480023541.01 | 0.834984 | 2.184334e-56 | 2.635089e-53 |
| MsG0380012146.01 | MsG0480023707.01 | 0.810722 | 1.000753e-50 | 6.078507e-48 |
| MsG0380012146.01 | MsG0480023832.01 | 0.839212 | 1.814158e-57 | 2.488001e-54 |
| MsG0380012146.01 | MsG0580024035.01 | 0.835031 | 2.125871e-56 | 2.567889e-53 |
| MsG0380012146.01 | MsG0580024478.01 | 0.828559 | 8.406979e-55 | 8.385621e-52 |
| MsG0380012146.01 | MsG0580024489.01 | 0.832534 | 8.950674e-56 | 1.003297e-52 |
| MsG0380012146.01 | MsG0580024758.01 | 0.812043 | 5.169932e-51 | 3.251986e-48 |
| MsG0380012146.01 | MsG0580025740.01 | 0.811328 | 7.397974e-51 | 4.565786e-48 |
| MsG0380012146.01 | MsG0580027049.01 | 0.817505 | 3.184663e-52 | 2.323580e-49 |
| MsG0380012146.01 | MsG0580027208.01 | 0.833372 | 5.538751e-56 | 6.366304e-53 |
| MsG0380012146.01 | MsG0580029712.01 | 0.832679 | 8.238307e-56 | 9.274497e-53 |
| MsG0380012146.01 | MsG0680030633.01 | 0.808341 | 3.247703e-50 | 1.852338e-47 |
| MsG0380012146.01 | MsG0680030763.01 | 0.804371 | 2.230699e-49 | 1.148184e-46 |
| MsG0380012146.01 | MsG0680034598.01 | 0.816081 | 6.644394e-52 | 4.662623e-49 |
| MsG0380012146.01 | MsG0780036752.01 | 0.806555 | 7.772418e-50 | 4.231324e-47 |
| MsG0380012146.01 | MsG0780036816.01 | 0.812328 | 4.481130e-51 | 2.840867e-48 |
| MsG0380012146.01 | MsG0780038837.01 | 0.810124 | 1.346864e-50 | 8.052192e-48 |
| MsG0380012146.01 | MsG0780039137.01 | 0.829085 | 6.269903e-55 | 6.350599e-52 |
| MsG0380012146.01 | MsG0780039462.01 | -0.812133 | 4.941343e-51 | 3.116047e-48 |
| MsG0380012146.01 | MsG0780040030.01 | 0.804437 | 2.161576e-49 | 1.114456e-46 |
| MsG0380012146.01 | MsG0780040488.01 | 0.807015 | 6.212298e-50 | 3.422301e-47 |
| MsG0380012146.01 | MsG0780040723.01 | 0.805444 | 1.330732e-49 | 7.041428e-47 |
| MsG0380012146.01 | MsG0780040896.01 | 0.830253 | 3.259100e-55 | 3.415581e-52 |
| MsG0380012146.01 | MsG0880041860.01 | 0.813301 | 2.743936e-51 | 1.785172e-48 |
| MsG0380012146.01 | MsG0880043299.01 | 0.809728 | 1.639544e-50 | 9.701019e-48 |
| MsG0380012146.01 | MsG0880043491.01 | 0.809418 | 1.910773e-50 | 1.121330e-47 |
| MsG0380012146.01 | MsG0880043516.01 | 0.817305 | 3.532352e-52 | 2.563025e-49 |
| MsG0380012146.01 | MsG0880045257.01 | 0.833412 | 5.412371e-56 | 6.228371e-53 |
| MsG0380012146.01 | MsG0880046012.01 | 0.826708 | 2.338753e-54 | 2.210236e-51 |
| MsG0380012146.01 | MsG0880046165.01 | 0.808845 | 2.534975e-50 | 1.464933e-47 |
| MsG0380012146.01 | MsG0880046788.01 | 0.814818 | 1.269319e-51 | 8.604646e-49 |
| MsG0380012146.01 | MsG0880047431.01 | 0.831963 | 1.239032e-55 | 1.365713e-52 |
| MsG0280007609.01 | MsG0380012146.01 | 0.816416 | 5.592664e-52 | 3.960366e-49 |
| MsG0280008280.01 | MsG0380012146.01 | 0.886560 | 3.075875e-72 | 2.207813e-68 |
| MsG0280008856.01 | MsG0380012146.01 | 0.800080 | 1.704073e-48 | 7.862995e-46 |
| MsG0280009514.01 | MsG0380012146.01 | 0.841371 | 4.949685e-58 | 7.257702e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012146.01.T01 | MTR_3g014660 | 81.707 | 246 | 30 | 3 | 1 | 241 | 94 | 329 | 1.23e-136 | 387 |
| MsG0380012146.01.T01 | MTR_8g039110 | 55.918 | 245 | 90 | 8 | 5 | 240 | 91 | 326 | 1.18e-80 | 245 |
| MsG0380012146.01.T01 | MTR_5g089750 | 49.133 | 173 | 83 | 1 | 41 | 213 | 249 | 416 | 2.03e-48 | 166 |
| MsG0380012146.01.T01 | MTR_0363s0040 | 55.963 | 109 | 39 | 3 | 5 | 112 | 91 | 191 | 3.37e-33 | 120 |
| MsG0380012146.01.T01 | MTR_8g076620 | 37.975 | 158 | 70 | 2 | 41 | 198 | 121 | 250 | 1.73e-32 | 120 |
| MsG0380012146.01.T01 | MTR_3g112510 | 41.104 | 163 | 69 | 6 | 41 | 198 | 86 | 226 | 3.10e-30 | 114 |
| MsG0380012146.01.T01 | MTR_5g021130 | 37.427 | 171 | 91 | 3 | 35 | 200 | 94 | 253 | 1.86e-29 | 112 |
| MsG0380012146.01.T01 | MTR_4g071110 | 33.742 | 163 | 71 | 3 | 41 | 203 | 17 | 142 | 5.46e-23 | 94.4 |
| MsG0380012146.01.T01 | MTR_1g023230 | 71.111 | 45 | 13 | 0 | 41 | 85 | 122 | 166 | 4.01e-17 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012146.01.T01 | AT5G12330 | 47.236 | 199 | 100 | 5 | 41 | 236 | 112 | 308 | 2.16e-48 | 162 |
| MsG0380012146.01.T01 | AT5G12330 | 47.236 | 199 | 100 | 5 | 41 | 236 | 112 | 308 | 2.16e-48 | 162 |
| MsG0380012146.01.T01 | AT5G12330 | 47.236 | 199 | 100 | 5 | 41 | 236 | 199 | 395 | 7.06e-48 | 163 |
| MsG0380012146.01.T01 | AT3G54430 | 44.809 | 183 | 69 | 5 | 29 | 210 | 31 | 182 | 3.52e-42 | 142 |
| MsG0380012146.01.T01 | AT3G51060 | 41.401 | 157 | 73 | 3 | 41 | 196 | 144 | 282 | 4.77e-35 | 128 |
| MsG0380012146.01.T01 | AT5G66350 | 44.025 | 159 | 70 | 4 | 41 | 198 | 120 | 260 | 4.89e-35 | 127 |
| MsG0380012146.01.T01 | AT5G66350 | 44.025 | 159 | 70 | 4 | 41 | 198 | 120 | 260 | 6.67e-35 | 127 |
| MsG0380012146.01.T01 | AT5G66350 | 44.025 | 159 | 70 | 4 | 41 | 198 | 120 | 260 | 8.58e-35 | 127 |
| MsG0380012146.01.T01 | AT1G75520 | 38.690 | 168 | 83 | 3 | 41 | 198 | 125 | 282 | 1.36e-31 | 119 |
| MsG0380012146.01.T01 | AT4G36260 | 45.062 | 162 | 74 | 3 | 41 | 198 | 94 | 244 | 1.85e-30 | 115 |
| MsG0380012146.01.T01 | AT2G18120 | 34.568 | 162 | 79 | 2 | 41 | 202 | 72 | 206 | 3.19e-29 | 109 |
| MsG0380012146.01.T01 | AT2G18120 | 35.152 | 165 | 76 | 3 | 41 | 202 | 72 | 208 | 4.05e-29 | 109 |
| MsG0380012146.01.T01 | AT2G21400 | 34.783 | 161 | 77 | 3 | 39 | 199 | 8 | 140 | 4.28e-27 | 103 |
| MsG0380012146.01.T01 | AT2G21400 | 34.783 | 161 | 77 | 3 | 39 | 199 | 7 | 139 | 5.05e-27 | 103 |
| MsG0380012146.01.T01 | AT2G21400 | 33.540 | 161 | 64 | 3 | 39 | 199 | 8 | 125 | 7.00e-26 | 99.8 |
| MsG0380012146.01.T01 | AT2G21400 | 33.540 | 161 | 64 | 3 | 39 | 199 | 7 | 124 | 7.75e-26 | 99.8 |
| MsG0380012146.01.T01 | AT5G12330 | 74.468 | 47 | 12 | 0 | 41 | 87 | 112 | 158 | 1.28e-20 | 87.4 |
| MsG0380012146.01.T01 | AT5G33210 | 48.611 | 72 | 37 | 0 | 41 | 112 | 17 | 88 | 2.93e-19 | 80.5 |
| MsG0380012146.01.T01 | AT1G19790 | 65.306 | 49 | 17 | 0 | 41 | 89 | 119 | 167 | 4.56e-19 | 85.1 |
| MsG0380012146.01.T01 | AT1G19790 | 65.306 | 49 | 17 | 0 | 41 | 89 | 119 | 167 | 4.56e-19 | 85.1 |
| MsG0380012146.01.T01 | AT5G33210 | 42.308 | 104 | 59 | 1 | 10 | 112 | 20 | 123 | 9.74e-19 | 80.9 |
Find 63 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGGGTAAACATTATTATT+TGG | 0.173024 | 3:+11589101 | None:intergenic |
| GGTGATGGTGAGGGTTGTTC+TGG | 0.272937 | 3:-11590199 | MsG0380012146.01.T01:CDS |
| GTTTAGATTGACAAGGAAAT+TGG | 0.288280 | 3:-11590000 | MsG0380012146.01.T01:intron |
| TTTGTCAAGATTGTGGTAAT+AGG | 0.301923 | 3:-11590375 | MsG0380012146.01.T01:CDS |
| TTTGGAAGATTCACTACTTC+AGG | 0.328387 | 3:+11590487 | None:intergenic |
| TTCATGCACCAGCTGTATTC+AGG | 0.332427 | 3:-11589325 | MsG0380012146.01.T01:CDS |
| CTCTATGATCATGGTGTTGA+TGG | 0.354571 | 3:-11589212 | MsG0380012146.01.T01:CDS |
| TGAAGATGAATTTGCTTACT+TGG | 0.385662 | 3:-11589273 | MsG0380012146.01.T01:CDS |
| AGAAGAGAATCATGGCAAAA+TGG | 0.391361 | 3:-11590446 | MsG0380012146.01.T01:CDS |
| TGCCAATAGCAGTTACTCTA+TGG | 0.398017 | 3:+11589300 | None:intergenic |
| GTGGAGATGTTTGCCGGTGG+TGG | 0.398827 | 3:-11590235 | MsG0380012146.01.T01:CDS |
| TTGTCAAGATTGTGGTAATA+GGG | 0.399150 | 3:-11590374 | MsG0380012146.01.T01:CDS |
| GAGATGTTTGCCGGTGGTGG+TGG | 0.399774 | 3:-11590232 | MsG0380012146.01.T01:CDS |
| ATGTTTGCCGGTGGTGGTGG+TGG | 0.402912 | 3:-11590229 | MsG0380012146.01.T01:CDS |
| AGGTTTCAAAGAGGGTTTAC+CGG | 0.403003 | 3:-11589354 | MsG0380012146.01.T01:CDS |
| TTGGCTACAGTTCATATTAG+TGG | 0.406244 | 3:-11589254 | MsG0380012146.01.T01:CDS |
| ACATACACATAGTACATGTT+TGG | 0.408164 | 3:+11589928 | None:intergenic |
| TTGTGTTTCAGAACTTCAAT+TGG | 0.409173 | 3:-11589174 | MsG0380012146.01.T01:CDS |
| AGGGAGGTGGAGATGTTTGC+CGG | 0.419024 | 3:-11590241 | MsG0380012146.01.T01:CDS |
| TTTGCCGGTGGTGGTGGTGG+TGG | 0.424345 | 3:-11590226 | MsG0380012146.01.T01:CDS |
| GTACTCATTTGAAGAGTACT+TGG | 0.429685 | 3:-11590288 | MsG0380012146.01.T01:CDS |
| TGGTGGTGGTGGTGATGGTG+AGG | 0.431191 | 3:-11590209 | MsG0380012146.01.T01:CDS |
| GATTCCTTCGACACGTCGTC+GGG | 0.433608 | 3:-11590266 | MsG0380012146.01.T01:CDS |
| AAGATGTAGGACTTGTTGTA+AGG | 0.436244 | 3:-11590329 | MsG0380012146.01.T01:CDS |
| GGTGGTGGTGGTGATGGTGA+GGG | 0.436973 | 3:-11590208 | MsG0380012146.01.T01:CDS |
| AAAGGGTTTCTCTATGATCA+TGG | 0.441459 | 3:-11589221 | MsG0380012146.01.T01:CDS |
| CATGGCTAGCTAGCAGAAGC+AGG | 0.449543 | 3:+11589083 | None:intergenic |
| TGCCATAGAGTAACTGCTAT+TGG | 0.452645 | 3:-11589302 | MsG0380012146.01.T01:CDS |
| GGTGAATGTTCTTCTGCAAT+TGG | 0.456180 | 3:-11589128 | MsG0380012146.01.T01:CDS |
| TCATGGCAAAATGGCTATGA+TGG | 0.462755 | 3:-11590437 | MsG0380012146.01.T01:CDS |
| ACATGTACTATGTGTATGTT+TGG | 0.466533 | 3:-11589924 | MsG0380012146.01.T01:CDS |
| CTTCGACACGTCGTCGGGAA+AGG | 0.472475 | 3:-11590261 | MsG0380012146.01.T01:CDS |
| AGTTCTGAAACACAAGGCAT+TGG | 0.476027 | 3:+11589182 | None:intergenic |
| AGTGAAGAAAATGGTGTGTA+TGG | 0.483753 | 3:-11590412 | MsG0380012146.01.T01:CDS |
| TTCGACACGTCGTCGGGAAA+GGG | 0.486782 | 3:-11590260 | MsG0380012146.01.T01:CDS |
| TGTGTTTCAGAACTTCAATT+GGG | 0.498389 | 3:-11589173 | MsG0380012146.01.T01:CDS |
| ACGTCGTCGGGAAAGGGAGG+TGG | 0.501203 | 3:-11590254 | MsG0380012146.01.T01:CDS |
| TTCTGGTGTTAAGAGACAAA+AGG | 0.501897 | 3:-11590182 | MsG0380012146.01.T01:intron |
| GGTTTCAAAGAGGGTTTACC+GGG | 0.502588 | 3:-11589353 | MsG0380012146.01.T01:CDS |
| ACATAGTACATGTTTGGAAT+CGG | 0.502921 | 3:+11589934 | None:intergenic |
| TATAGAGTTTGTCAAGATTG+TGG | 0.512497 | 3:-11590382 | MsG0380012146.01.T01:CDS |
| AATAATTGTAGTGGAAAGAA+TGG | 0.512653 | 3:-11589149 | MsG0380012146.01.T01:CDS |
| ATGATGGAGAGTGAAGAAAA+TGG | 0.542008 | 3:-11590421 | MsG0380012146.01.T01:CDS |
| TATGTGTATGTTTGGTATCA+CGG | 0.548478 | 3:-11589916 | MsG0380012146.01.T01:intron |
| GGATTCCTTCGACACGTCGT+CGG | 0.554911 | 3:-11590267 | MsG0380012146.01.T01:CDS |
| GCAGATGCAGGTTTCAAAGA+GGG | 0.558109 | 3:-11589362 | MsG0380012146.01.T01:intron |
| CTATGGCACCTGAATACAGC+TGG | 0.563710 | 3:+11589317 | None:intergenic |
| ATCACTGAAGAAGAGAATCA+TGG | 0.572196 | 3:-11590454 | MsG0380012146.01.T01:CDS |
| CTTTCCCGACGACGTGTCGA+AGG | 0.578431 | 3:+11590262 | None:intergenic |
| AGAGTAACTGCTATTGGCAA+TGG | 0.579028 | 3:-11589296 | MsG0380012146.01.T01:CDS |
| CAATTGGGAAATAATTGTAG+TGG | 0.580003 | 3:-11589158 | MsG0380012146.01.T01:CDS |
| AGATGTAGGACTTGTTGTAA+GGG | 0.582676 | 3:-11590328 | MsG0380012146.01.T01:CDS |
| AGAGAAACCCTTTGAAAACA+TGG | 0.595856 | 3:+11589231 | None:intergenic |
| ATTGTGTGTTTAGAAGATGT+AGG | 0.602227 | 3:-11590342 | MsG0380012146.01.T01:CDS |
| TCTATGATCATGGTGTTGAT+GGG | 0.608318 | 3:-11589211 | MsG0380012146.01.T01:CDS |
| TGCAGATGCAGGTTTCAAAG+AGG | 0.617800 | 3:-11589363 | MsG0380012146.01.T01:intron |
| TTTAAATGTTTAGATTGACA+AGG | 0.629535 | 3:-11590007 | MsG0380012146.01.T01:intron |
| AGGACTTGTTGTAAGGGACG+TGG | 0.632558 | 3:-11590322 | MsG0380012146.01.T01:CDS |
| GCTGGTGCATGAACATGACC+CGG | 0.653334 | 3:+11589335 | None:intergenic |
| GACACGTCGTCGGGAAAGGG+AGG | 0.654658 | 3:-11590257 | MsG0380012146.01.T01:CDS |
| ATGGCTAGCTAGCAGAAGCA+GGG | 0.656680 | 3:+11589084 | None:intergenic |
| GAGGTGGAGATGTTTGCCGG+TGG | 0.707877 | 3:-11590238 | MsG0380012146.01.T01:CDS |
| AATTGAAGTTCTGAAACACA+AGG | 0.729820 | 3:+11589176 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CCATAAATTAATATGATAAG+AGG | - | Chr3:11590133-11590152 | MsG0380012146.01.T01:intron | 20.0% |
| !! | CCTCTTATCATATTAATTTA+TGG | + | Chr3:11590136-11590155 | None:intergenic | 20.0% |
| !! | CTAATGTAATAAATTCTTTG+CGG | + | Chr3:11589999-11590018 | None:intergenic | 20.0% |
| !! | TATGTTTGATATTACACAAT+TGG | + | Chr3:11589793-11589812 | None:intergenic | 20.0% |
| !! | TTTAAATGTTTAGATTGACA+AGG | - | Chr3:11589574-11589593 | MsG0380012146.01.T01:intron | 20.0% |
| !!! | TCAATTTTGATAATTGTTTG+AGG | + | Chr3:11590083-11590102 | None:intergenic | 20.0% |
| ! | AAGAGACAAAAGGTTTATTA+GGG | - | Chr3:11589409-11589428 | MsG0380012146.01.T01:intron | 25.0% |
| ! | TAAGAGACAAAAGGTTTATT+AGG | - | Chr3:11589408-11589427 | MsG0380012146.01.T01:intron | 25.0% |
| !! | AATAATTGTAGTGGAAAGAA+TGG | - | Chr3:11590432-11590451 | MsG0380012146.01.T01:CDS | 25.0% |
| !! | TTTGATAATTGTTTGAGGTA+GGG | + | Chr3:11590078-11590097 | None:intergenic | 25.0% |
| !!! | TTTTGATAATTGTTTGAGGT+AGG | + | Chr3:11590079-11590098 | None:intergenic | 25.0% |
| ACATACACATAGTACATGTT+TGG | + | Chr3:11589656-11589675 | None:intergenic | 30.0% | |
| ACATGTACTATGTGTATGTT+TGG | - | Chr3:11589657-11589676 | MsG0380012146.01.T01:intron | 30.0% | |
| AGTAGTGAATCTTCCAAAAA+AGG | - | Chr3:11589099-11589118 | MsG0380012146.01.T01:CDS | 30.0% | |
| ATTGTGTGTTTAGAAGATGT+AGG | - | Chr3:11589239-11589258 | MsG0380012146.01.T01:CDS | 30.0% | |
| CAATTGGGAAATAATTGTAG+TGG | - | Chr3:11590423-11590442 | MsG0380012146.01.T01:CDS | 30.0% | |
| GCAGGGTAAACATTATTATT+TGG | + | Chr3:11590483-11590502 | None:intergenic | 30.0% | |
| GTAGTGAATCTTCCAAAAAA+GGG | - | Chr3:11589100-11589119 | MsG0380012146.01.T01:CDS | 30.0% | |
| GTTTAGATTGACAAGGAAAT+TGG | - | Chr3:11589581-11589600 | MsG0380012146.01.T01:intron | 30.0% | |
| TAAGAGGAATTTAAGTGTCT+TGG | - | Chr3:11590149-11590168 | MsG0380012146.01.T01:intron | 30.0% | |
| TATAGAGTTTGTCAAGATTG+TGG | - | Chr3:11589199-11589218 | MsG0380012146.01.T01:CDS | 30.0% | |
| TATGTGTATGTTTGGTATCA+CGG | - | Chr3:11589665-11589684 | MsG0380012146.01.T01:intron | 30.0% | |
| TGTGTTTCAGAACTTCAATT+GGG | - | Chr3:11590408-11590427 | MsG0380012146.01.T01:CDS | 30.0% | |
| TTGTCAAGATTGTGGTAATA+GGG | - | Chr3:11589207-11589226 | MsG0380012146.01.T01:CDS | 30.0% | |
| TTGTGTTTCAGAACTTCAAT+TGG | - | Chr3:11590407-11590426 | MsG0380012146.01.T01:CDS | 30.0% | |
| TTTGTCAAGATTGTGGTAAT+AGG | - | Chr3:11589206-11589225 | MsG0380012146.01.T01:CDS | 30.0% | |
| ! | ACATAGTACATGTTTGGAAT+CGG | + | Chr3:11589650-11589669 | None:intergenic | 30.0% |
| ! | TAGTATTTTCTCCTTCATCA+GGG | - | Chr3:11590028-11590047 | MsG0380012146.01.T01:intron | 30.0% |
| ! | TGAAGATGAATTTGCTTACT+TGG | - | Chr3:11590308-11590327 | MsG0380012146.01.T01:CDS | 30.0% |
| ! | TTAGTATTTTCTCCTTCATC+AGG | - | Chr3:11590027-11590046 | MsG0380012146.01.T01:intron | 30.0% |
| !! | AAAAGTAAGTGAAAGTACCT+TGG | + | Chr3:11589518-11589537 | None:intergenic | 30.0% |
| !! | AATTGAAGTTCTGAAACACA+AGG | + | Chr3:11590408-11590427 | None:intergenic | 30.0% |
| AAAGGGTTTCTCTATGATCA+TGG | - | Chr3:11590360-11590379 | MsG0380012146.01.T01:CDS | 35.0% | |
| AGAAGAGAATCATGGCAAAA+TGG | - | Chr3:11589135-11589154 | MsG0380012146.01.T01:CDS | 35.0% | |
| AGTGAAGAAAATGGTGTGTA+TGG | - | Chr3:11589169-11589188 | MsG0380012146.01.T01:CDS | 35.0% | |
| ATCACTGAAGAAGAGAATCA+TGG | - | Chr3:11589127-11589146 | MsG0380012146.01.T01:CDS | 35.0% | |
| TCAGCAACCTTAATTTCATG+TGG | + | Chr3:11590179-11590198 | None:intergenic | 35.0% | |
| TTGGCTACAGTTCATATTAG+TGG | - | Chr3:11590327-11590346 | MsG0380012146.01.T01:CDS | 35.0% | |
| TTTGGAAGATTCACTACTTC+AGG | + | Chr3:11589097-11589116 | None:intergenic | 35.0% | |
| ! | AGAGAAACCCTTTGAAAACA+TGG | + | Chr3:11590353-11590372 | None:intergenic | 35.0% |
| ! | ATGATGGAGAGTGAAGAAAA+TGG | - | Chr3:11589160-11589179 | MsG0380012146.01.T01:CDS | 35.0% |
| ! | ATTAGTGGCCATGTTTTCAA+AGG | - | Chr3:11590342-11590361 | MsG0380012146.01.T01:CDS | 35.0% |
| ! | CCACTTGATTTTCAAAAAGC+GGG | + | Chr3:11589751-11589770 | None:intergenic | 35.0% |
| ! | CCCACTTGATTTTCAAAAAG+CGG | + | Chr3:11589752-11589771 | None:intergenic | 35.0% |
| ! | TTAGTGGCCATGTTTTCAAA+GGG | - | Chr3:11590343-11590362 | MsG0380012146.01.T01:CDS | 35.0% |
| ! | TTCTGGTGTTAAGAGACAAA+AGG | - | Chr3:11589399-11589418 | MsG0380012146.01.T01:intron | 35.0% |
| !! | AAGATGTAGGACTTGTTGTA+AGG | - | Chr3:11589252-11589271 | MsG0380012146.01.T01:CDS | 35.0% |
| !! | AGATGTAGGACTTGTTGTAA+GGG | - | Chr3:11589253-11589272 | MsG0380012146.01.T01:CDS | 35.0% |
| !! | GTACTCATTTGAAGAGTACT+TGG | - | Chr3:11589293-11589312 | MsG0380012146.01.T01:CDS | 35.0% |
| !! | TCTATGATCATGGTGTTGAT+GGG | - | Chr3:11590370-11590389 | MsG0380012146.01.T01:CDS | 35.0% |
| !! | TCTCATAGCTCTAACTCTAA+TGG | - | Chr3:11589456-11589475 | MsG0380012146.01.T01:intron | 35.0% |
| !!! | CCGCTTTTTGAAAATCAAGT+GGG | - | Chr3:11589749-11589768 | MsG0380012146.01.T01:intron | 35.0% |
| AGAGTAACTGCTATTGGCAA+TGG | - | Chr3:11590285-11590304 | MsG0380012146.01.T01:CDS | 40.0% | |
| AGGTTTCAAAGAGGGTTTAC+CGG | - | Chr3:11590227-11590246 | MsG0380012146.01.T01:CDS | 40.0% | |
| ATCAGGGTTTGAACGAATTC+TGG | - | Chr3:11590044-11590063 | MsG0380012146.01.T01:intron | 40.0% | |
| ATTCGTTCAAACCCTGATGA+AGG | + | Chr3:11590042-11590061 | None:intergenic | 40.0% | |
| CATATGACTATGTGAATGCG+TGG | - | Chr3:11589964-11589983 | MsG0380012146.01.T01:CDS | 40.0% | |
| GAGCTAGTAGCAAAACTCTT+TGG | + | Chr3:11589489-11589508 | None:intergenic | 40.0% | |
| GAGGAATTTAAGTGTCTTGG+AGG | - | Chr3:11590152-11590171 | MsG0380012146.01.T01:intron | 40.0% | |
| GGTGAATGTTCTTCTGCAAT+TGG | - | Chr3:11590453-11590472 | MsG0380012146.01.T01:CDS | 40.0% | |
| TCATGGCAAAATGGCTATGA+TGG | - | Chr3:11589144-11589163 | MsG0380012146.01.T01:CDS | 40.0% | |
| TGCCAATAGCAGTTACTCTA+TGG | + | Chr3:11590284-11590303 | None:intergenic | 40.0% | |
| TGCCATAGAGTAACTGCTAT+TGG | - | Chr3:11590279-11590298 | MsG0380012146.01.T01:CDS | 40.0% | |
| TGGAGGACCACATGAAATTA+AGG | - | Chr3:11590169-11590188 | MsG0380012146.01.T01:intron | 40.0% | |
| TTCTGTTGAATTGCAGATGC+AGG | - | Chr3:11590207-11590226 | MsG0380012146.01.T01:CDS | 40.0% | |
| ! | CTCTATGATCATGGTGTTGA+TGG | - | Chr3:11590369-11590388 | MsG0380012146.01.T01:CDS | 40.0% |
| !! | AGTTCTGAAACACAAGGCAT+TGG | + | Chr3:11590402-11590421 | None:intergenic | 40.0% |
| !!! | CCCGCTTTTTGAAAATCAAG+TGG | - | Chr3:11589748-11589767 | MsG0380012146.01.T01:intron | 40.0% |
| !!! | TCAGTGATCACTCCCTTTTT+TGG | + | Chr3:11589115-11589134 | None:intergenic | 40.0% |
| GCAGATGCAGGTTTCAAAGA+GGG | - | Chr3:11590219-11590238 | MsG0380012146.01.T01:CDS | 45.0% | |
| GGTTTCAAAGAGGGTTTACC+GGG | - | Chr3:11590228-11590247 | MsG0380012146.01.T01:CDS | 45.0% | |
| TGCAGATGCAGGTTTCAAAG+AGG | - | Chr3:11590218-11590237 | MsG0380012146.01.T01:CDS | 45.0% | |
| TTCATGCACCAGCTGTATTC+AGG | - | Chr3:11590256-11590275 | MsG0380012146.01.T01:CDS | 45.0% | |
| CTATGGCACCTGAATACAGC+TGG | + | Chr3:11590267-11590286 | None:intergenic | 50.0% | |
| ! | GCTACTAGCTCTTGTCACCA+AGG | - | Chr3:11589498-11589517 | MsG0380012146.01.T01:intron | 50.0% |
| !! | AGGACTTGTTGTAAGGGACG+TGG | - | Chr3:11589259-11589278 | MsG0380012146.01.T01:CDS | 50.0% |
| AGGGAGGTGGAGATGTTTGC+CGG | - | Chr3:11589340-11589359 | MsG0380012146.01.T01:CDS | 55.0% | |
| GATTCCTTCGACACGTCGTC+GGG | - | Chr3:11589315-11589334 | MsG0380012146.01.T01:CDS | 55.0% | |
| GCTGGTGCATGAACATGACC+CGG | + | Chr3:11590249-11590268 | None:intergenic | 55.0% | |
| GGATTCCTTCGACACGTCGT+CGG | - | Chr3:11589314-11589333 | MsG0380012146.01.T01:CDS | 55.0% | |
| TTCGACACGTCGTCGGGAAA+GGG | - | Chr3:11589321-11589340 | MsG0380012146.01.T01:CDS | 55.0% | |
| ! | GGTGATGGTGAGGGTTGTTC+TGG | - | Chr3:11589382-11589401 | MsG0380012146.01.T01:intron | 55.0% |
| CTTCGACACGTCGTCGGGAA+AGG | - | Chr3:11589320-11589339 | MsG0380012146.01.T01:CDS | 60.0% | |
| CTTTCCCGACGACGTGTCGA+AGG | + | Chr3:11589322-11589341 | None:intergenic | 60.0% | |
| GAGGTGGAGATGTTTGCCGG+TGG | - | Chr3:11589343-11589362 | MsG0380012146.01.T01:CDS | 60.0% | |
| GTGGAGATGTTTGCCGGTGG+TGG | - | Chr3:11589346-11589365 | MsG0380012146.01.T01:CDS | 60.0% | |
| ! | GAGATGTTTGCCGGTGGTGG+TGG | - | Chr3:11589349-11589368 | MsG0380012146.01.T01:CDS | 60.0% |
| !! | ATGTTTGCCGGTGGTGGTGG+TGG | - | Chr3:11589352-11589371 | MsG0380012146.01.T01:CDS | 60.0% |
| !! | GGTGGTGGTGGTGATGGTGA+GGG | - | Chr3:11589373-11589392 | MsG0380012146.01.T01:intron | 60.0% |
| !! | TGGTGGTGGTGGTGATGGTG+AGG | - | Chr3:11589372-11589391 | MsG0380012146.01.T01:intron | 60.0% |
| ACCACCACCACCACCACCAC+CGG | + | Chr3:11589362-11589381 | None:intergenic | 65.0% | |
| ACGTCGTCGGGAAAGGGAGG+TGG | - | Chr3:11589327-11589346 | MsG0380012146.01.T01:CDS | 65.0% | |
| GACACGTCGTCGGGAAAGGG+AGG | - | Chr3:11589324-11589343 | MsG0380012146.01.T01:CDS | 65.0% | |
| !! | GGTGGTGGTGGTGGTGGTGA+TGG | - | Chr3:11589367-11589386 | MsG0380012146.01.T01:intron | 65.0% |
| !! | TTTGCCGGTGGTGGTGGTGG+TGG | - | Chr3:11589355-11589374 | MsG0380012146.01.T01:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGTGGTGG+TGG | - | Chr3:11589361-11589380 | MsG0380012146.01.T01:CDS | 70.0% |
| !! | GCCGGTGGTGGTGGTGGTGG+TGG | - | Chr3:11589358-11589377 | MsG0380012146.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 11589088 | 11590515 | 11589088 | ID=MsG0380012146.01;Name=MsG0380012146.01 |
| Chr3 | mRNA | 11589088 | 11590515 | 11589088 | ID=MsG0380012146.01.T01;Parent=MsG0380012146.01;Name=MsG0380012146.01.T01;_AED=0.43;_eAED=0.51;_QI=0|0|0|1|1|1|3|0|241 |
| Chr3 | exon | 11590183 | 11590515 | 11590183 | ID=MsG0380012146.01.T01:exon:2043;Parent=MsG0380012146.01.T01 |
| Chr3 | exon | 11589917 | 11590016 | 11589917 | ID=MsG0380012146.01.T01:exon:2042;Parent=MsG0380012146.01.T01 |
| Chr3 | exon | 11589088 | 11589380 | 11589088 | ID=MsG0380012146.01.T01:exon:2041;Parent=MsG0380012146.01.T01 |
| Chr3 | CDS | 11590183 | 11590515 | 11590183 | ID=MsG0380012146.01.T01:cds;Parent=MsG0380012146.01.T01 |
| Chr3 | CDS | 11589917 | 11590016 | 11589917 | ID=MsG0380012146.01.T01:cds;Parent=MsG0380012146.01.T01 |
| Chr3 | CDS | 11589088 | 11589380 | 11589088 | ID=MsG0380012146.01.T01:cds;Parent=MsG0380012146.01.T01 |
| Gene Sequence |
| Protein sequence |
