AlfalfaGEDB | MsG0480023280.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023280.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023280.01.T01	XP_003630173.1	86.607	112	7	2	1	104	14	125	4.74E-57	182

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023280.01.T01	G7LC90	86.607	112	7	2	1	104	14	125	2.26e-57	182

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048177.01	MsG0480023280.01	0.829308	5.534567e-55	5.644077e-52
MsG0180000019.01	MsG0480023280.01	0.819598	1.067775e-52	8.253598e-50
MsG0180000157.01	MsG0480023280.01	0.805833	1.102641e-49	5.893332e-47
MsG0180000197.01	MsG0480023280.01	0.811423	7.053254e-51	4.364392e-48
MsG0180001043.01	MsG0480023280.01	0.847664	1.002058e-59	1.794114e-56
MsG0180001154.01	MsG0480023280.01	0.811332	7.382270e-51	4.556644e-48
MsG0180001922.01	MsG0480023280.01	0.800572	1.353352e-48	6.323034e-46
MsG0180003088.01	MsG0480023280.01	0.817288	3.563736e-52	2.584608e-49
MsG0180003241.01	MsG0480023280.01	0.869706	2.532741e-66	9.596254e-63
MsG0180003252.01	MsG0480023280.01	0.810884	9.232497e-51	5.632158e-48
MsG0180004762.01	MsG0480023280.01	0.801867	7.350754e-49	3.548978e-46
MsG0180004809.01	MsG0480023280.01	0.807413	5.117776e-50	2.848702e-47
MsG0180005125.01	MsG0480023280.01	0.847693	9.838110e-60	1.763106e-56
MsG0180006016.01	MsG0480023280.01	0.820734	5.866264e-53	4.679733e-50
MsG0280011235.01	MsG0480023280.01	0.801895	7.254068e-49	3.504791e-46
MsG0280011288.01	MsG0480023280.01	0.802763	4.808752e-49	2.375199e-46
MsG0280011473.01	MsG0480023280.01	0.840729	7.294700e-58	1.048543e-54
MsG0380011754.01	MsG0480023280.01	0.813435	2.563495e-51	1.673968e-48
MsG0380014680.01	MsG0480023280.01	0.821426	4.063486e-53	3.304868e-50
MsG0380014913.01	MsG0480023280.01	0.812331	4.473825e-51	2.836562e-48
MsG0480018904.01	MsG0480023280.01	0.847354	1.219629e-59	2.161850e-56
MsG0480020445.01	MsG0480023280.01	0.815041	1.132760e-51	7.725394e-49
MsG0480021695.01	MsG0480023280.01	0.814129	1.802420e-51	1.199327e-48
MsG0480022480.01	MsG0480023280.01	0.807311	5.376716e-50	2.985002e-47
MsG0480022775.01	MsG0480023280.01	0.805100	1.571019e-49	8.239687e-47
MsG0480023280.01	MsG0480023417.01	0.973736	6.165245e-137	8.091319e-131
MsG0480023280.01	MsG0480023517.01	0.865732	4.769683e-65	1.568281e-61
MsG0480023280.01	MsG0480023947.01	0.804691	1.912730e-49	9.926928e-47
MsG0480023280.01	MsG0580024204.01	0.804759	1.851140e-49	9.624472e-47
MsG0480023280.01	MsG0580025134.01	0.817186	3.756690e-52	2.716955e-49
MsG0480023280.01	MsG0580025278.01	0.834611	2.712471e-56	3.235591e-53
MsG0480023280.01	MsG0580025453.01	0.835993	1.214129e-56	1.509681e-53
MsG0480023280.01	MsG0580025769.01	0.835529	1.590994e-56	1.950799e-53
MsG0480023280.01	MsG0580026011.01	0.813050	3.114206e-51	2.012327e-48
MsG0480023280.01	MsG0580027034.01	0.804463	2.133888e-49	1.100944e-46
MsG0480023280.01	MsG0580027073.01	0.812433	4.250140e-51	2.702157e-48
MsG0480023280.01	MsG0580027345.01	0.816651	4.953791e-52	3.530822e-49
MsG0480023280.01	MsG0580028185.01	0.805570	1.252455e-49	6.648514e-47
MsG0480023280.01	MsG0580028416.01	0.838759	2.375743e-57	3.213398e-54
MsG0480023280.01	MsG0580028453.01	0.838276	3.166388e-57	4.219596e-54
MsG0480023280.01	MsG0580029758.01	0.829744	4.337822e-55	4.480057e-52
MsG0480023280.01	MsG0580030160.01	0.806197	9.244689e-50	4.987215e-47
MsG0480023280.01	MsG0680031538.01	0.833473	5.226297e-56	6.024850e-53
MsG0480023280.01	MsG0680034672.01	0.833148	6.298767e-56	7.191568e-53
MsG0480023280.01	MsG0680035646.01	0.819330	1.229094e-52	9.429023e-50
MsG0480023280.01	MsG0780036291.01	0.818273	2.135994e-52	1.591530e-49
MsG0480023280.01	MsG0780037258.01	0.827193	1.790901e-54	1.716707e-51
MsG0480023280.01	MsG0780037503.01	0.807060	6.077826e-50	3.352134e-47
MsG0480023280.01	MsG0780038444.01	0.831116	2.003322e-55	2.153185e-52
MsG0480023280.01	MsG0780040516.01	0.822574	2.202767e-53	1.851062e-50
MsG0480023280.01	MsG0780040679.01	0.838258	3.200033e-57	4.262176e-54
MsG0480023280.01	MsG0780041196.01	0.806919	6.509029e-50	3.576895e-47
MsG0480023280.01	MsG0780041357.01	0.815022	1.143490e-51	7.794791e-49
MsG0480023280.01	MsG0880041985.01	0.807136	5.856846e-50	3.236565e-47
MsG0480023280.01	MsG0880042811.01	0.805609	1.229186e-49	6.531565e-47
MsG0480023280.01	MsG0880042843.01	0.816442	5.517347e-52	3.909699e-49
MsG0480023280.01	MsG0880043001.01	0.815809	7.643109e-52	5.323466e-49
MsG0480023280.01	MsG0880043082.01	0.824348	8.475956e-54	7.487825e-51
MsG0480023280.01	MsG0880043279.01	0.829524	4.905738e-55	5.033453e-52
MsG0480023280.01	MsG0880043286.01	0.865233	6.850036e-65	2.212498e-61
MsG0480023280.01	MsG0880043288.01	0.858288	9.132461e-63	2.319182e-59
MsG0480023280.01	MsG0880045106.01	0.841428	4.781572e-58	7.024061e-55
MsG0480023280.01	MsG0880045914.01	0.807230	5.594680e-50	3.099361e-47
MsG0480023280.01	MsG0880046058.01	0.829229	5.783961e-55	5.884107e-52
MsG0480023280.01	MsG0880046113.01	0.822999	1.753324e-53	1.491144e-50
MsG0480023280.01	MsG0880046209.01	0.806883	6.626739e-50	3.638123e-47
MsG0480023280.01	MsG0880046244.01	0.812680	3.752543e-51	2.401201e-48
MsG0480023280.01	MsG0880046329.01	0.820802	5.656703e-53	4.521448e-50
MsG0480023280.01	MsG0880046720.01	0.809706	1.657626e-50	9.802024e-48
MsG0480023280.01	MsG0880046731.01	0.842649	2.273088e-58	3.468985e-55
MsG0480023280.01	MsG0880046888.01	0.857999	1.112681e-62	2.798528e-59
MsG0480023280.01	MsG0880047081.01	0.807340	5.301734e-50	2.945505e-47
MsG0480023280.01	MsG0880047444.01	0.837021	6.644979e-57	8.522538e-54
MsG0480023280.01	MsG0880047590.01	0.821220	4.533754e-53	3.666401e-50
MsG0380015209.01	MsG0480023280.01	0.817644	2.963711e-52	2.170472e-49
MsG0380015488.01	MsG0480023280.01	0.827481	1.527538e-54	1.476582e-51
MsG0380015686.01	MsG0480023280.01	0.868912	4.588592e-66	1.688773e-62
MsG0380015687.01	MsG0480023280.01	0.813364	2.657309e-51	1.731904e-48
MsG0380017020.01	MsG0480023280.01	0.826651	2.413707e-54	2.277345e-51
MsG0380017158.01	MsG0480023280.01	0.826826	2.192511e-54	2.079053e-51
MsG0380017226.01	MsG0480023280.01	0.828489	8.739236e-55	8.699699e-52
MsG0380017286.01	MsG0480023280.01	0.832634	8.450525e-56	9.500899e-53
MsG0280007258.01	MsG0480023280.01	0.806286	8.853967e-50	4.787211e-47
MsG0280007272.01	MsG0480023280.01	0.833688	4.619352e-56	5.359474e-53
MsG0280007369.01	MsG0480023280.01	0.803920	2.768442e-49	1.408651e-46
MsG0280007439.01	MsG0480023280.01	0.820813	5.624878e-53	4.497314e-50
MsG0280007549.01	MsG0480023280.01	0.832092	1.151433e-55	1.273857e-52
MsG0280007714.01	MsG0480023280.01	0.813257	2.805421e-51	1.822954e-48
MsG0280008220.01	MsG0480023280.01	0.822721	2.035352e-53	1.717436e-50
MsG0280009235.01	MsG0480023280.01	0.841516	4.533011e-58	6.676992e-55
MsG0280009838.01	MsG0480023280.01	0.800447	1.434914e-48	6.682849e-46
MsG0280010275.01	MsG0480023280.01	-0.805126	1.551757e-49	8.144275e-47
MsG0280010276.01	MsG0480023280.01	-0.830473	2.879166e-55	3.036367e-52
MsG0280010299.01	MsG0480023280.01	0.802355	5.835697e-49	2.852717e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023280.01.T01	MTR_8g092580	86.607	112	7	2	1	104	14	125	5.74e-61	182

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 15 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAAGATACAAGAGATCTT+TGG	0.301924	4:+83157012	MsG0480023280.01.T01:CDS
CGATGCAGCACAAAAGCTTA+TGG	0.339207	4:+83156876	MsG0480023280.01.T01:CDS
CTCACCCTTGTATCATTAAT+AGG	0.341960	4:-83157114	None:intergenic
CAAAGATACAAGAGATCTTT+GGG	0.370184	4:+83157013	MsG0480023280.01.T01:CDS
TTCTCTTGTGAATATTTACA+TGG	0.470533	4:+83157083	MsG0480023280.01.T01:CDS
CTACACCTATTAATGATACA+AGG	0.485559	4:+83157109	MsG0480023280.01.T01:CDS
AAGGGCAACAACAACATTAG+AGG	0.505052	4:+83156916	MsG0480023280.01.T01:CDS
ACAAAGTGATGATATTGTAA+TGG	0.520018	4:+83156990	MsG0480023280.01.T01:CDS
CGAAGCAACCGACATGAAGA+AGG	0.528099	4:+83156958	MsG0480023280.01.T01:CDS
GGATCTTAGTGATGAAGAGA+AGG	0.549113	4:+83156897	MsG0480023280.01.T01:CDS
TGTATCATTAATAGGTGTAG+TGG	0.560392	4:-83157106	None:intergenic
TTGGCGTAAGATGAAGATTG+TGG	0.597571	4:+83156819	None:intergenic
GATCTTAGTGATGAAGAGAA+GGG	0.605452	4:+83156898	MsG0480023280.01.T01:CDS
TACACCTATTAATGATACAA+GGG	0.645512	4:+83157110	MsG0480023280.01.T01:CDS
TTAGAGGCAGAAGAAGCAAG+AGG	0.689009	4:+83156932	MsG0480023280.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACAAAGTGATGATATTGTAA+TGG	+	Chr4:83156990-83157009	MsG0480023280.01.T01:CDS	25.0%
!	TACACCTATTAATGATACAA+GGG	+	Chr4:83157110-83157129	MsG0480023280.01.T01:CDS	25.0%
!	TTCTCTTGTGAATATTTACA+TGG	+	Chr4:83157083-83157102	MsG0480023280.01.T01:CDS	25.0%
!!!	CTTTTTTGCTTCTTAACTAT+AGG	-	Chr4:83157057-83157076	None:intergenic	25.0%
	CAAAGATACAAGAGATCTTT+GGG	+	Chr4:83157013-83157032	MsG0480023280.01.T01:CDS	30.0%
	CTACACCTATTAATGATACA+AGG	+	Chr4:83157109-83157128	MsG0480023280.01.T01:CDS	30.0%
	TGTATCATTAATAGGTGTAG+TGG	-	Chr4:83157109-83157128	None:intergenic	30.0%
	CTCACCCTTGTATCATTAAT+AGG	-	Chr4:83157117-83157136	None:intergenic	35.0%
	GATCTTAGTGATGAAGAGAA+GGG	+	Chr4:83156898-83156917	MsG0480023280.01.T01:CDS	35.0%
	GCAAAGATACAAGAGATCTT+TGG	+	Chr4:83157012-83157031	MsG0480023280.01.T01:CDS	35.0%
!!!	GCTTTTTTCCTTCTTCATGT+CGG	-	Chr4:83156969-83156988	None:intergenic	35.0%
	AAGGGCAACAACAACATTAG+AGG	+	Chr4:83156916-83156935	MsG0480023280.01.T01:CDS	40.0%
	GGATCTTAGTGATGAAGAGA+AGG	+	Chr4:83156897-83156916	MsG0480023280.01.T01:CDS	40.0%
	CGATGCAGCACAAAAGCTTA+TGG	+	Chr4:83156876-83156895	MsG0480023280.01.T01:CDS	45.0%
	TTAGAGGCAGAAGAAGCAAG+AGG	+	Chr4:83156932-83156951	MsG0480023280.01.T01:CDS	45.0%
	CGAAGCAACCGACATGAAGA+AGG	+	Chr4:83156958-83156977	MsG0480023280.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	83156829	83157143	83156829	ID=MsG0480023280.01;Name=MsG0480023280.01
Chr4	mRNA	83156829	83157143	83156829	ID=MsG0480023280.01.T01;Parent=MsG0480023280.01;Name=MsG0480023280.01.T01;_AED=0.30;_eAED=0.30;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|104
Chr4	exon	83156829	83157143	83156829	ID=MsG0480023280.01.T01:exon:21504;Parent=MsG0480023280.01.T01
Chr4	CDS	83156829	83157143	83156829	ID=MsG0480023280.01.T01:cds;Parent=MsG0480023280.01.T01

Gene Sequence	>MsG0480023280.01.T01 ATGAAGATTGTGGAATATGATCAACACTTGATTAATGAGTATGATGTCGATGCAGCACAAAAGCTTATGGATCTTAGTGATGAAGAGAAGGGCAACAACAACATTAGAGGCAGAAGAAGCAAGAGGATGCGAAGCAACCGACATGAAGAAGGAAAAAAGCAACAAAGTGATGATATTGTAATGGCAAAGATACAAGAGATCTTTGGGAAAGATGTTGAAGTTTTTCCTATAGTTAAGAAGCAAAAAAGATATCGTTCTCTTGTGAATATTTACATGGCCACTACACCTATTAATGATACAAGGGTGAGTGCATGA
Protein sequence	>MsG0480023280.01.T01 MKIVEYDQHLINEYDVDAAQKLMDLSDEEKGNNNIRGRRSKRMRSNRHEEGKKQQSDDIVMAKIQEIFGKDVEVFPIVKKQKRYRSLVNIYMATTPINDTRVSA*