Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023869.01.T01 | KEH21367.1 | 75 | 92 | 21 | 1 | 1 | 92 | 1 | 90 | 1.02E-36 | 133 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023869.01.T01 | I3TAH2 | 75.000 | 92 | 21 | 1 | 1 | 92 | 1 | 90 | 1.01e-36 | 134 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0480023869.01 | -0.803481 | 3.415766e-49 | 1.718417e-46 |
| MsG0080049013.01 | MsG0480023869.01 | 0.808196 | 3.487698e-50 | 1.981608e-47 |
| MsG0080049059.01 | MsG0480023869.01 | -0.830183 | 3.389225e-55 | 3.544823e-52 |
| MsG0180002489.01 | MsG0480023869.01 | -0.815085 | 1.107332e-51 | 7.560875e-49 |
| MsG0180002491.01 | MsG0480023869.01 | -0.809025 | 2.320285e-50 | 1.347298e-47 |
| MsG0180002940.01 | MsG0480023869.01 | 0.808371 | 3.200522e-50 | 1.826840e-47 |
| MsG0180003409.01 | MsG0480023869.01 | -0.843679 | 1.208263e-58 | 1.904882e-55 |
| MsG0180004335.01 | MsG0480023869.01 | 0.808172 | 3.528881e-50 | 2.003775e-47 |
| MsG0180006013.01 | MsG0480023869.01 | -0.828328 | 9.556133e-55 | 9.467637e-52 |
| MsG0180006069.01 | MsG0480023869.01 | -0.811512 | 6.745865e-51 | 4.184081e-48 |
| MsG0180006226.01 | MsG0480023869.01 | 0.806579 | 7.681665e-50 | 4.184326e-47 |
| MsG0380012073.01 | MsG0480023869.01 | -0.806938 | 6.450908e-50 | 3.546608e-47 |
| MsG0380012471.01 | MsG0480023869.01 | -0.818837 | 1.591500e-52 | 1.204548e-49 |
| MsG0480020065.01 | MsG0480023869.01 | -0.822488 | 2.305831e-53 | 1.932789e-50 |
| MsG0480020372.01 | MsG0480023869.01 | -0.831540 | 1.575391e-55 | 1.714908e-52 |
| MsG0480020791.01 | MsG0480023869.01 | -0.803822 | 2.901800e-49 | 1.472694e-46 |
| MsG0480021448.01 | MsG0480023869.01 | -0.841991 | 3.395745e-58 | 5.076596e-55 |
| MsG0480021453.01 | MsG0480023869.01 | -0.807023 | 6.187333e-50 | 3.409275e-47 |
| MsG0480022052.01 | MsG0480023869.01 | 0.803030 | 4.234784e-49 | 2.105847e-46 |
| MsG0480022151.01 | MsG0480023869.01 | 0.846296 | 2.374651e-59 | 4.068245e-56 |
| MsG0480022167.01 | MsG0480023869.01 | -0.802088 | 6.623423e-49 | 3.215659e-46 |
| MsG0480022183.01 | MsG0480023869.01 | -0.806550 | 7.790555e-50 | 4.240691e-47 |
| MsG0480022480.01 | MsG0480023869.01 | -0.801591 | 8.374928e-49 | 4.014926e-46 |
| MsG0480023013.01 | MsG0480023869.01 | -0.807427 | 5.080630e-50 | 2.829084e-47 |
| MsG0480023361.01 | MsG0480023869.01 | -0.837949 | 3.842003e-57 | 5.070064e-54 |
| MsG0480023559.01 | MsG0480023869.01 | -0.803233 | 3.844881e-49 | 1.921911e-46 |
| MsG0480023707.01 | MsG0480023869.01 | -0.810556 | 1.086816e-50 | 6.571755e-48 |
| MsG0480023869.01 | MsG0580024458.01 | 0.859829 | 3.155260e-63 | 8.446906e-60 |
| MsG0480023869.01 | MsG0580025134.01 | -0.824954 | 6.099165e-54 | 5.483542e-51 |
| MsG0480023869.01 | MsG0580026157.01 | -0.821112 | 4.801255e-53 | 3.870892e-50 |
| MsG0480023869.01 | MsG0580029661.01 | -0.815879 | 7.373935e-52 | 5.145458e-49 |
| MsG0480023869.01 | MsG0580029668.01 | 0.869783 | 2.389299e-66 | 9.076494e-63 |
| MsG0480023869.01 | MsG0580030160.01 | -0.818224 | 2.191718e-52 | 1.630841e-49 |
| MsG0480023869.01 | MsG0680031549.01 | -0.801030 | 1.091390e-48 | 5.159022e-46 |
| MsG0480023869.01 | MsG0680031651.01 | -0.821476 | 3.957307e-53 | 3.222830e-50 |
| MsG0480023869.01 | MsG0680034061.01 | -0.841312 | 5.128208e-58 | 7.504917e-55 |
| MsG0480023869.01 | MsG0680035777.01 | -0.805352 | 1.391324e-49 | 7.344574e-47 |
| MsG0480023869.01 | MsG0780038645.01 | -0.829818 | 4.159789e-55 | 4.305795e-52 |
| MsG0480023869.01 | MsG0780039137.01 | -0.823812 | 1.131968e-53 | 9.851201e-51 |
| MsG0480023869.01 | MsG0780039841.01 | -0.838179 | 3.354122e-57 | 4.456515e-54 |
| MsG0480023869.01 | MsG0780039950.01 | -0.801871 | 7.338364e-49 | 3.543323e-46 |
| MsG0480023869.01 | MsG0780040699.01 | 0.819590 | 1.072080e-52 | 8.285306e-50 |
| MsG0480023869.01 | MsG0780040861.01 | -0.850962 | 1.209219e-60 | 2.409772e-57 |
| MsG0480023869.01 | MsG0780040929.01 | -0.802539 | 5.348725e-49 | 2.626786e-46 |
| MsG0480023869.01 | MsG0780041295.01 | -0.845812 | 3.214820e-59 | 5.423323e-56 |
| MsG0480023869.01 | MsG0780041490.01 | -0.812302 | 4.539903e-51 | 2.876025e-48 |
| MsG0480023869.01 | MsG0880041883.01 | -0.827580 | 1.446130e-54 | 1.401744e-51 |
| MsG0480023869.01 | MsG0880042349.01 | -0.802894 | 4.517425e-49 | 2.238743e-46 |
| MsG0480023869.01 | MsG0880042779.01 | -0.804185 | 2.439222e-49 | 1.249470e-46 |
| MsG0480023869.01 | MsG0880042811.01 | -0.811717 | 6.087003e-51 | 3.795730e-48 |
| MsG0480023869.01 | MsG0880043465.01 | -0.811098 | 8.297474e-51 | 5.090377e-48 |
| MsG0480023869.01 | MsG0880045631.01 | -0.811893 | 5.573677e-51 | 3.491786e-48 |
| MsG0480023869.01 | MsG0880046177.01 | -0.831551 | 1.565296e-55 | 1.704467e-52 |
| MsG0480023869.01 | MsG0880046762.01 | -0.805984 | 1.024941e-49 | 5.499378e-47 |
| MsG0480023869.01 | MsG0880046933.01 | -0.852271 | 5.151360e-61 | 1.070753e-57 |
| MsG0480023869.01 | MsG0880047191.01 | -0.817986 | 2.481080e-52 | 1.834124e-49 |
| MsG0480023869.01 | MsG0880047607.01 | -0.802678 | 5.005054e-49 | 2.466729e-46 |
| MsG0380015793.01 | MsG0480023869.01 | 0.880988 | 3.487019e-70 | 2.007309e-66 |
| MsG0380016389.01 | MsG0480023869.01 | -0.848820 | 4.803933e-60 | 8.926077e-57 |
| MsG0380017135.01 | MsG0480023869.01 | 0.836793 | 7.597105e-57 | 9.677054e-54 |
| MsG0380017998.01 | MsG0480023869.01 | -0.900010 | 1.122531e-77 | 1.423613e-73 |
| MsG0380018001.01 | MsG0480023869.01 | -0.812585 | 3.936669e-51 | 2.512746e-48 |
| MsG0280006480.01 | MsG0480023869.01 | 0.831750 | 1.398126e-55 | 1.531520e-52 |
| MsG0280007024.01 | MsG0480023869.01 | -0.817100 | 3.928273e-52 | 2.834345e-49 |
| MsG0280007133.01 | MsG0480023869.01 | 0.823802 | 1.138392e-53 | 9.903942e-51 |
| MsG0280007607.01 | MsG0480023869.01 | -0.805877 | 1.079462e-49 | 5.775757e-47 |
| MsG0280007609.01 | MsG0480023869.01 | -0.845638 | 3.583641e-59 | 6.010865e-56 |
| MsG0280007715.01 | MsG0480023869.01 | -0.841154 | 5.645409e-58 | 8.221521e-55 |
| MsG0280008286.01 | MsG0480023869.01 | -0.807435 | 5.061198e-50 | 2.818755e-47 |
| MsG0280008327.01 | MsG0480023869.01 | -0.819992 | 8.678002e-53 | 6.782216e-50 |
| MsG0280008885.01 | MsG0480023869.01 | -0.832715 | 8.069226e-56 | 9.094269e-53 |
| MsG0280009109.01 | MsG0480023869.01 | -0.821171 | 4.652798e-53 | 3.757360e-50 |
| MsG0280009140.01 | MsG0480023869.01 | -0.838699 | 2.462636e-57 | 3.324895e-54 |
| MsG0280010002.01 | MsG0480023869.01 | -0.820449 | 6.818209e-53 | 5.396715e-50 |
| MsG0280010389.01 | MsG0480023869.01 | -0.829568 | 4.786993e-55 | 4.917711e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023869.01.T01 | MTR_8g103890 | 75.000 | 92 | 21 | 1 | 1 | 92 | 1 | 90 | 1.24e-40 | 133 |
| MsG0480023869.01.T01 | MTR_8g103890 | 75.000 | 92 | 21 | 1 | 1 | 92 | 1 | 90 | 2.38e-40 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGTGGTGGTGGTGGATA+TGG | 0.185076 | 4:+90689615 | None:intergenic |
| GTTGCAACGAATAGAGATTA+AGG | 0.266313 | 4:+90689672 | MsG0480023868.01.T01:CDS |
| TGAAGTGGCGAAATGGAAAT+TGG | 0.324166 | 4:+90689701 | MsG0480023868.01.T01:CDS |
| ATCCCTTTCCACAAGATTGA+TGG | 0.332314 | 4:+90689757 | MsG0480023868.01.T01:CDS |
| AATGAACCTTGGTTAATTGT+TGG | 0.402656 | 4:-90689574 | MsG0480023869.01.T01:CDS |
| TATGGATGCAGCTGAGGAAT+TGG | 0.407153 | 4:+90689633 | None:intergenic |
| TGTGGGTTCAGTGGTGGTGG+TGG | 0.425197 | 4:+90689609 | None:intergenic |
| TTGCAACGAATAGAGATTAA+GGG | 0.435700 | 4:+90689673 | MsG0480023868.01.T01:CDS |
| ATCCATCAATCTTGTGGAAA+GGG | 0.446330 | 4:-90689759 | MsG0480023869.01.T01:CDS |
| TAACCAAGGTTCATTCTGCT+TGG | 0.447500 | 4:+90689582 | None:intergenic |
| GGTTTAGTGAAGTGGCGAAA+TGG | 0.478131 | 4:+90689694 | MsG0480023868.01.T01:CDS |
| TATTTCAGGTGGGTTTGAGA+TGG | 0.497079 | 4:-90689421 | MsG0480023869.01.T01:intron |
| AGATTAAGGGTTTAGTGAAG+TGG | 0.503605 | 4:+90689686 | MsG0480023868.01.T01:CDS |
| ATTGTTGGCCTCGGTAATCC+CGG | 0.504322 | 4:-90689559 | MsG0480023869.01.T01:CDS |
| CCTTGGTTAATTGTTGGCCT+CGG | 0.505176 | 4:-90689568 | MsG0480023869.01.T01:CDS |
| TGATGAATCCATCAATCTTG+TGG | 0.535462 | 4:-90689765 | MsG0480023869.01.T01:CDS |
| AATCCATCAATCTTGTGGAA+AGG | 0.548100 | 4:-90689760 | MsG0480023869.01.T01:CDS |
| AATCCAAGCAGAATGAACCT+TGG | 0.632059 | 4:-90689585 | MsG0480023869.01.T01:CDS |
| GGTGGATATGGATGCAGCTG+AGG | 0.648622 | 4:+90689627 | None:intergenic |
| TGGAAAGGGATAATGAACGT+TGG | 0.652970 | 4:-90689745 | MsG0480023869.01.T01:CDS |
| ATGCAGCTGAGGAATTGGAA+TGG | 0.661833 | 4:+90689638 | MsG0480023868.01.T01:CDS |
| CCGAGGCCAACAATTAACCA+AGG | 0.685507 | 4:+90689568 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAGAAAAAGAAAAAAG+GGG | + | Chr4:90689413-90689432 | None:intergenic | 20.0% |
| !! | GACAAAAGAAAAAGAAAAAA+GGG | + | Chr4:90689414-90689433 | None:intergenic | 20.0% |
| !! | TGACAAAAGAAAAAGAAAAA+AGG | + | Chr4:90689415-90689434 | None:intergenic | 20.0% |
| !!! | TTTTTCTTTTTCTTTTGTCA+GGG | - | Chr4:90689415-90689434 | MsG0480023869.01.T01:CDS | 20.0% |
| !!! | TTTTTTCTTTTTCTTTTGTC+AGG | - | Chr4:90689414-90689433 | MsG0480023869.01.T01:CDS | 20.0% |
| ! | CAAAAGAAAAAGAAAAAAGG+GGG | + | Chr4:90689412-90689431 | None:intergenic | 25.0% |
| !!! | CTGTTTTTATTATTTCAGGT+GGG | - | Chr4:90689803-90689822 | MsG0480023869.01.T01:five_prime_UTR | 25.0% |
| !!! | GACTCTGTTTTTATTATTTC+AGG | - | Chr4:90689799-90689818 | MsG0480023869.01.T01:five_prime_UTR | 25.0% |
| !!! | TCTGTTTTTATTATTTCAGG+TGG | - | Chr4:90689802-90689821 | MsG0480023869.01.T01:five_prime_UTR | 25.0% |
| AATGAACCTTGGTTAATTGT+TGG | - | Chr4:90689660-90689679 | MsG0480023869.01.T01:CDS | 30.0% | |
| TGAATGTAGTACTAACATTG+TGG | + | Chr4:90689722-90689741 | MsG0480023868.01.T01:CDS | 30.0% | |
| TTGCAACGAATAGAGATTAA+GGG | + | Chr4:90689564-90689583 | None:intergenic | 30.0% | |
| AATCCATCAATCTTGTGGAA+AGG | - | Chr4:90689474-90689493 | MsG0480023869.01.T01:intron | 35.0% | |
| AGATTAAGGGTTTAGTGAAG+TGG | + | Chr4:90689551-90689570 | None:intergenic | 35.0% | |
| ATCCATCAATCTTGTGGAAA+GGG | - | Chr4:90689475-90689494 | MsG0480023869.01.T01:intron | 35.0% | |
| GTTGCAACGAATAGAGATTA+AGG | + | Chr4:90689565-90689584 | None:intergenic | 35.0% | |
| TGATGAATCCATCAATCTTG+TGG | - | Chr4:90689469-90689488 | MsG0480023869.01.T01:intron | 35.0% | |
| ! | TCATTCTGCTTGGATTTTGT+GGG | + | Chr4:90689645-90689664 | MsG0480023868.01.T01:CDS | 35.0% |
| ! | TGTACCATTGAACTTTTTGC+CGG | + | Chr4:90689697-90689716 | MsG0480023868.01.T01:CDS | 35.0% |
| ! | TTCATTCTGCTTGGATTTTG+TGG | + | Chr4:90689646-90689665 | MsG0480023868.01.T01:CDS | 35.0% |
| AATCCAAGCAGAATGAACCT+TGG | - | Chr4:90689649-90689668 | MsG0480023869.01.T01:CDS | 40.0% | |
| AATCCCGGCAAAAAGTTCAA+TGG | - | Chr4:90689690-90689709 | MsG0480023869.01.T01:CDS | 40.0% | |
| ATCCCTTTCCACAAGATTGA+TGG | + | Chr4:90689480-90689499 | None:intergenic | 40.0% | |
| TAACCAAGGTTCATTCTGCT+TGG | + | Chr4:90689655-90689674 | MsG0480023868.01.T01:CDS | 40.0% | |
| TGAAGTGGCGAAATGGAAAT+TGG | + | Chr4:90689536-90689555 | None:intergenic | 40.0% | |
| TGGAAAGGGATAATGAACGT+TGG | - | Chr4:90689489-90689508 | MsG0480023869.01.T01:intron | 40.0% | |
| ! | GTACCATTGAACTTTTTGCC+GGG | + | Chr4:90689696-90689715 | MsG0480023868.01.T01:CDS | 40.0% |
| ! | TATTTCAGGTGGGTTTGAGA+TGG | - | Chr4:90689813-90689832 | MsG0480023869.01.T01:five_prime_UTR | 40.0% |
| ATGCAGCTGAGGAATTGGAA+TGG | + | Chr4:90689599-90689618 | None:intergenic | 45.0% | |
| GGTTTAGTGAAGTGGCGAAA+TGG | + | Chr4:90689543-90689562 | None:intergenic | 45.0% | |
| TATGGATGCAGCTGAGGAAT+TGG | + | Chr4:90689604-90689623 | None:intergenic | 45.0% | |
| ! | CCTTGGTTAATTGTTGGCCT+CGG | - | Chr4:90689666-90689685 | MsG0480023869.01.T01:CDS | 45.0% |
| ! | CTTGGATTTTGTGGGTTCAG+TGG | + | Chr4:90689637-90689656 | MsG0480023868.01.T01:CDS | 45.0% |
| CCGAGGCCAACAATTAACCA+AGG | + | Chr4:90689669-90689688 | MsG0480023868.01.T01:CDS | 50.0% | |
| ! | ACTTTTTGCCGGGATTACCG+AGG | + | Chr4:90689686-90689705 | MsG0480023868.01.T01:CDS | 50.0% |
| ! | ATTGTTGGCCTCGGTAATCC+CGG | - | Chr4:90689675-90689694 | MsG0480023869.01.T01:CDS | 50.0% |
| ! | GGATTTTGTGGGTTCAGTGG+TGG | + | Chr4:90689634-90689653 | MsG0480023868.01.T01:CDS | 50.0% |
| ! | TTTTGTGGGTTCAGTGGTGG+TGG | + | Chr4:90689631-90689650 | None:intergenic | 50.0% |
| !! | TTCAGTGGTGGTGGTGGATA+TGG | + | Chr4:90689622-90689641 | None:intergenic | 50.0% |
| GGTGGATATGGATGCAGCTG+AGG | + | Chr4:90689610-90689629 | None:intergenic | 55.0% | |
| !! | TGTGGGTTCAGTGGTGGTGG+TGG | + | Chr4:90689628-90689647 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 90689406 | 90689850 | 90689406 | ID=MsG0480023869.01;Name=MsG0480023869.01 |
| Chr4 | mRNA | 90689406 | 90689850 | 90689406 | ID=MsG0480023869.01.T01;Parent=MsG0480023869.01;Name=MsG0480023869.01.T01;_AED=0.25;_eAED=0.28;_QI=65|1|0.85|1|0.5|0.57|7|0|94 |
| Chr4 | exon | 90689406 | 90689435 | 90689406 | ID=MsG0480023869.01.T01:exon:33876;Parent=MsG0480023869.01.T01 |
| Chr4 | exon | 90689531 | 90689850 | 90689531 | ID=MsG0480023869.01.T01:exon:33875;Parent=MsG0480023869.01.T01 |
| Chr4 | five_prime_UTR | 90689786 | 90689850 | 90689786 | ID=MsG0480023869.01.T01:five_prime_utr;Parent=MsG0480023869.01.T01 |
| Chr4 | CDS | 90689531 | 90689785 | 90689531 | ID=MsG0480023869.01.T01:cds;Parent=MsG0480023869.01.T01 |
| Chr4 | CDS | 90689406 | 90689435 | 90689406 | ID=MsG0480023869.01.T01:cds;Parent=MsG0480023869.01.T01 |
| Gene Sequence |
| Protein sequence |
