Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025821.01.T01 | XP_003613278.1 | 96.986 | 365 | 8 | 2 | 1 | 365 | 1 | 362 | 0 | 729 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025821.01.T01 | K4PW38 | 36.444 | 225 | 105 | 7 | 14 | 227 | 31 | 228 | 9.87E-36 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025821.01.T01 | G7K2R0 | 96.986 | 365 | 8 | 2 | 1 | 365 | 1 | 362 | 0.0 | 729 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000145.01 | MsG0580025821.01 | 0.807175 | 5.746901e-50 | 3.179124e-47 |
| MsG0180004088.01 | MsG0580025821.01 | 0.819396 | 1.187433e-52 | 9.126473e-50 |
| MsG0180005524.01 | MsG0580025821.01 | 0.819456 | 1.150359e-52 | 8.857244e-50 |
| MsG0380011496.01 | MsG0580025821.01 | 0.830941 | 2.211514e-55 | 2.364804e-52 |
| MsG0380011501.01 | MsG0580025821.01 | 0.837814 | 4.161726e-57 | 5.469359e-54 |
| MsG0380012067.01 | MsG0580025821.01 | 0.808272 | 3.359319e-50 | 1.912560e-47 |
| MsG0380013475.01 | MsG0580025821.01 | 0.812073 | 5.093862e-51 | 3.206911e-48 |
| MsG0380014506.01 | MsG0580025821.01 | 0.803396 | 3.556941e-49 | 1.785500e-46 |
| MsG0380014706.01 | MsG0580025821.01 | 0.814806 | 1.276945e-51 | 8.653853e-49 |
| MsG0480018653.01 | MsG0580025821.01 | 0.816691 | 4.854330e-52 | 3.463370e-49 |
| MsG0480018909.01 | MsG0580025821.01 | 0.816647 | 4.964143e-52 | 3.537752e-49 |
| MsG0480019711.01 | MsG0580025821.01 | 0.801579 | 8.425957e-49 | 4.038171e-46 |
| MsG0480020765.01 | MsG0580025821.01 | 0.816124 | 6.499282e-52 | 4.565966e-49 |
| MsG0480020788.01 | MsG0580025821.01 | 0.809923 | 1.488207e-50 | 8.850713e-48 |
| MsG0480020832.01 | MsG0580025821.01 | 0.838372 | 2.991317e-57 | 3.998395e-54 |
| MsG0480020935.01 | MsG0580025821.01 | -0.825954 | 3.536490e-54 | 3.271331e-51 |
| MsG0480021195.01 | MsG0580025821.01 | 0.804147 | 2.483305e-49 | 1.270886e-46 |
| MsG0480021796.01 | MsG0580025821.01 | 0.808740 | 2.669939e-50 | 1.538598e-47 |
| MsG0480022029.01 | MsG0580025821.01 | 0.801227 | 9.945199e-49 | 4.724266e-46 |
| MsG0480022615.01 | MsG0580025821.01 | -0.827982 | 1.157744e-54 | 1.135651e-51 |
| MsG0480023172.01 | MsG0580025821.01 | 0.833165 | 6.237886e-56 | 7.125444e-53 |
| MsG0480023251.01 | MsG0580025821.01 | 0.817515 | 3.167687e-52 | 2.311864e-49 |
| MsG0480023936.01 | MsG0580025821.01 | -0.822476 | 2.321066e-53 | 1.944881e-50 |
| MsG0580024790.01 | MsG0580025821.01 | -0.833502 | 5.139407e-56 | 5.929912e-53 |
| MsG0580025407.01 | MsG0580025821.01 | -0.852067 | 5.886770e-61 | 1.215641e-57 |
| MsG0580025477.01 | MsG0580025821.01 | 0.814968 | 1.175943e-51 | 8.003890e-49 |
| MsG0580025821.01 | MsG0580025987.01 | 0.820327 | 7.271983e-53 | 5.736049e-50 |
| MsG0580025821.01 | MsG0580027320.01 | 0.818046 | 2.404587e-52 | 1.780493e-49 |
| MsG0580025821.01 | MsG0580027925.01 | 0.863319 | 2.710524e-64 | 8.183183e-61 |
| MsG0580025821.01 | MsG0580029236.01 | 0.810318 | 1.223667e-50 | 7.352564e-48 |
| MsG0580025821.01 | MsG0680030881.01 | 0.804875 | 1.750751e-49 | 9.130355e-47 |
| MsG0580025821.01 | MsG0680031707.01 | 0.800100 | 1.688528e-48 | 7.794988e-46 |
| MsG0580025821.01 | MsG0680034739.01 | 0.808908 | 2.458089e-50 | 1.422854e-47 |
| MsG0580025821.01 | MsG0680035658.01 | 0.802139 | 6.465315e-49 | 3.142936e-46 |
| MsG0580025821.01 | MsG0780036593.01 | 0.863253 | 2.840275e-64 | 8.555275e-61 |
| MsG0580025821.01 | MsG0780036596.01 | 0.856159 | 3.884501e-62 | 9.185038e-59 |
| MsG0580025821.01 | MsG0780039139.01 | 0.808231 | 3.428954e-50 | 1.949986e-47 |
| MsG0580025821.01 | MsG0780039443.01 | 0.804252 | 2.362309e-49 | 1.212137e-46 |
| MsG0580025821.01 | MsG0780039867.01 | -0.829038 | 6.436892e-55 | 6.510416e-52 |
| MsG0580025821.01 | MsG0780040576.01 | -0.811386 | 7.184272e-51 | 4.440984e-48 |
| MsG0580025821.01 | MsG0780040751.01 | 0.803457 | 3.455196e-49 | 1.737133e-46 |
| MsG0580025821.01 | MsG0780040834.01 | 0.801875 | 7.324740e-49 | 3.537134e-46 |
| MsG0580025821.01 | MsG0780040888.01 | 0.827864 | 1.235950e-54 | 1.208137e-51 |
| MsG0580025821.01 | MsG0880041861.01 | 0.822193 | 2.699949e-53 | 2.243991e-50 |
| MsG0580025821.01 | MsG0880041862.01 | 0.802113 | 6.543910e-49 | 3.179045e-46 |
| MsG0580025821.01 | MsG0880041864.01 | 0.803001 | 4.293393e-49 | 2.133386e-46 |
| MsG0580025821.01 | MsG0880043037.01 | 0.819397 | 1.186905e-52 | 9.122768e-50 |
| MsG0580025821.01 | MsG0880043157.01 | 0.827013 | 1.977129e-54 | 1.885140e-51 |
| MsG0580025821.01 | MsG0880043937.01 | 0.832793 | 7.719555e-56 | 8.719800e-53 |
| MsG0580025821.01 | MsG0880043941.01 | 0.814740 | 1.320533e-51 | 8.933179e-49 |
| MsG0580025821.01 | MsG0880045235.01 | 0.805970 | 1.032273e-49 | 5.536554e-47 |
| MsG0380015577.01 | MsG0580025821.01 | 0.821794 | 3.340603e-53 | 2.745248e-50 |
| MsG0380015875.01 | MsG0580025821.01 | 0.801527 | 8.633816e-49 | 4.132462e-46 |
| MsG0380017202.01 | MsG0580025821.01 | 0.813887 | 2.038429e-51 | 1.347515e-48 |
| MsG0480018236.01 | MsG0580025821.01 | 0.803702 | 3.073344e-49 | 1.554886e-46 |
| MsG0280006601.01 | MsG0580025821.01 | 0.803959 | 2.718027e-49 | 1.384327e-46 |
| MsG0280007414.01 | MsG0580025821.01 | 0.816351 | 5.782953e-52 | 4.088150e-49 |
| MsG0280007432.01 | MsG0580025821.01 | 0.813229 | 2.844540e-51 | 1.846960e-48 |
| MsG0280007955.01 | MsG0580025821.01 | 0.818251 | 2.160479e-52 | 1.608808e-49 |
| MsG0280008377.01 | MsG0580025821.01 | 0.830114 | 3.523779e-55 | 3.677993e-52 |
| MsG0280008378.01 | MsG0580025821.01 | 0.802583 | 5.236524e-49 | 2.574598e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025821.01.T01 | MTR_5g034840 | 96.986 | 365 | 8 | 2 | 1 | 365 | 1 | 362 | 0.0 | 729 |
| MsG0580025821.01.T01 | MTR_1g018810 | 51.185 | 211 | 80 | 5 | 13 | 217 | 11 | 204 | 8.16e-70 | 221 |
| MsG0580025821.01.T01 | MTR_2g078750 | 38.537 | 205 | 99 | 6 | 14 | 218 | 20 | 197 | 5.50e-36 | 135 |
| MsG0580025821.01.T01 | MTR_2g078750 | 40.000 | 155 | 73 | 3 | 64 | 218 | 1 | 135 | 3.68e-28 | 113 |
| MsG0580025821.01.T01 | MTR_2g078750 | 42.553 | 94 | 54 | 0 | 125 | 218 | 11 | 104 | 4.80e-18 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025821.01.T01 | AT1G60060 | 69.041 | 365 | 63 | 11 | 1 | 337 | 1 | 343 | 1.75e-160 | 455 |
| MsG0580025821.01.T01 | AT1G60060 | 64.688 | 320 | 63 | 11 | 46 | 337 | 6 | 303 | 1.07e-123 | 360 |
| MsG0580025821.01.T01 | AT5G53900 | 38.333 | 240 | 118 | 7 | 14 | 244 | 21 | 239 | 9.51e-39 | 142 |
| MsG0580025821.01.T01 | AT3G15240 | 37.073 | 205 | 107 | 4 | 14 | 218 | 34 | 216 | 1.10e-37 | 139 |
| MsG0580025821.01.T01 | AT5G53900 | 39.844 | 128 | 67 | 2 | 126 | 244 | 1 | 127 | 2.53e-20 | 89.7 |
| MsG0580025821.01.T01 | AT5G53900 | 39.844 | 128 | 67 | 2 | 126 | 244 | 1 | 127 | 2.53e-20 | 89.7 |
| MsG0580025821.01.T01 | AT2G31280 | 30.928 | 194 | 83 | 9 | 15 | 207 | 6 | 149 | 2.19e-14 | 75.1 |
| MsG0580025821.01.T01 | AT2G31280 | 30.928 | 194 | 83 | 9 | 15 | 207 | 6 | 149 | 2.51e-14 | 74.7 |
| MsG0580025821.01.T01 | AT2G27230 | 27.488 | 211 | 105 | 8 | 13 | 218 | 4 | 171 | 3.11e-13 | 71.2 |
| MsG0580025821.01.T01 | AT2G27230 | 27.488 | 211 | 105 | 8 | 13 | 218 | 4 | 171 | 3.11e-13 | 71.2 |
Find 101 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAGCTCATCATCAGTTTA+TGG | 0.128324 | 5:-24689098 | MsG0580025821.01.T01:CDS |
| ATAAAATAATTAATAATTAT+TGG | 0.163550 | 5:+24687166 | None:intergenic |
| TGCATATTTCAGTTTCTTCT+TGG | 0.196042 | 5:-24687390 | MsG0580025821.01.T01:three_prime_UTR |
| CAATCACCAGTGTTTATTTC+AGG | 0.239507 | 5:+24689122 | None:intergenic |
| TCACATGAGATCTATAACTA+TGG | 0.263874 | 5:-24689017 | MsG0580025821.01.T01:CDS |
| CACACATTTGCATGGAATAA+TGG | 0.268865 | 5:+24687325 | None:intergenic |
| TTGGGTGGAGGGTAGTTTCT+AGG | 0.275012 | 5:+24689587 | None:intergenic |
| AGAGAAGGTGTTGTTCAATT+AGG | 0.283607 | 5:-24688451 | MsG0580025821.01.T01:CDS |
| CAAAGAGCATTGGAATTTAC+AGG | 0.285652 | 5:-24687704 | MsG0580025821.01.T01:CDS |
| AACACAAAATCAACATGTTT+TGG | 0.289389 | 5:-24687741 | MsG0580025821.01.T01:CDS |
| AAATTAATTTCTTGATCATT+AGG | 0.297602 | 5:+24688820 | None:intergenic |
| CATTTCCATGATGATCTTGA+TGG | 0.308916 | 5:+24687561 | None:intergenic |
| AATGGATCAAATAATAATAA+TGG | 0.318152 | 5:-24687542 | MsG0580025821.01.T01:CDS |
| TATATGCATGCACAGGATAT+TGG | 0.324411 | 5:-24689186 | MsG0580025821.01.T01:intron |
| AGGATATTGGTTTGGGAAGA+TGG | 0.329021 | 5:-24689173 | MsG0580025821.01.T01:CDS |
| TCTTGAAACCCTTTAGCTAT+AGG | 0.329720 | 5:+24689830 | None:intergenic |
| ATTGATGGAGTTACTTTCAT+TGG | 0.336300 | 5:+24687830 | None:intergenic |
| TCAGTTCTTGTCTGGTATAA+AGG | 0.362612 | 5:-24688615 | MsG0580025821.01.T01:intron |
| CTAGCTCCTGCTGCTGTTCA+AGG | 0.368140 | 5:-24687210 | MsG0580025821.01.T01:three_prime_UTR |
| CATCAAGATCATCATGGAAA+TGG | 0.376848 | 5:-24687560 | MsG0580025821.01.T01:CDS |
| CCAATCTACCACATTGTACT+TGG | 0.379695 | 5:-24687247 | MsG0580025821.01.T01:three_prime_UTR |
| GCATGCACAGGATATTGGTT+TGG | 0.384339 | 5:-24689181 | MsG0580025821.01.T01:intron |
| TCACCAACATCAAGTTGTTC+AGG | 0.392523 | 5:+24687623 | None:intergenic |
| GACATCTTGAAGAAAAGTTC+AGG | 0.396788 | 5:+24689038 | None:intergenic |
| CATGCACAGGATATTGGTTT+GGG | 0.400265 | 5:-24689180 | MsG0580025821.01.T01:intron |
| TTCAGTTTCTTCTTGGACCT+TGG | 0.401107 | 5:-24687383 | MsG0580025821.01.T01:three_prime_UTR |
| TCATCATCAGCATACCCTTA+TGG | 0.403770 | 5:-24687947 | MsG0580025821.01.T01:CDS |
| TAAAGATACCTATAGCTAAA+GGG | 0.404162 | 5:-24689838 | MsG0580025821.01.T01:five_prime_UTR |
| GTTGGTGTGTGCTTATATAA+AGG | 0.405928 | 5:-24689892 | None:intergenic |
| GCTTCAAGACTACTCATTGA+TGG | 0.406362 | 5:+24687815 | None:intergenic |
| TTAAAGATACCTATAGCTAA+AGG | 0.409220 | 5:-24689839 | MsG0580025821.01.T01:five_prime_UTR |
| TGGGAAGCTCAGTTCTTGTC+TGG | 0.414012 | 5:-24688623 | MsG0580025821.01.T01:CDS |
| CTAGCTCATCATCAGTTTAT+GGG | 0.418675 | 5:-24689097 | MsG0580025821.01.T01:CDS |
| CTCTCTCACTACAATATGTT+AGG | 0.427654 | 5:+24687458 | None:intergenic |
| TAGGTATCTTTAATAATAGT+AGG | 0.427738 | 5:+24689849 | None:intergenic |
| TTTACAGCACCCTAGGACTT+GGG | 0.440440 | 5:-24688642 | MsG0580025821.01.T01:intron |
| CAAGAGGAAACAGAAGGAAT+TGG | 0.444449 | 5:-24689328 | MsG0580025821.01.T01:intron |
| TGTTCTGATGGACCACCATA+AGG | 0.450763 | 5:+24687932 | None:intergenic |
| CATCCATGAAAATTCTCAAT+GGG | 0.458791 | 5:-24689636 | MsG0580025821.01.T01:CDS |
| ATTGTAGTGAGAGAGTTGTT+AGG | 0.462280 | 5:-24687450 | MsG0580025821.01.T01:three_prime_UTR |
| GGTAGTGTATTGTAGTATGA+AGG | 0.462747 | 5:-24687362 | MsG0580025821.01.T01:three_prime_UTR |
| CCAAGTACAATGTGGTAGAT+TGG | 0.469859 | 5:+24687247 | None:intergenic |
| GCATCCATGAAAATTCTCAA+TGG | 0.475544 | 5:-24689637 | MsG0580025821.01.T01:CDS |
| CTATGATCTTCTTACTTGGG+TGG | 0.478789 | 5:+24689572 | None:intergenic |
| TGATCTTCTTACTTGGGTGG+AGG | 0.479359 | 5:+24689575 | None:intergenic |
| GAACAATGGCACAACTTTCA+AGG | 0.484632 | 5:-24687914 | MsG0580025821.01.T01:CDS |
| GTTGGATAATGAAGAAGAAG+AGG | 0.487343 | 5:-24687654 | MsG0580025821.01.T01:CDS |
| AACTGAGCTTCCCAAGTCCT+AGG | 0.490005 | 5:+24688632 | None:intergenic |
| AGCTACATTGAAAGCATACC+AGG | 0.501005 | 5:-24687989 | MsG0580025821.01.T01:CDS |
| GTTCTGATGGACCACCATAA+GGG | 0.509808 | 5:+24687933 | None:intergenic |
| GAGATCTATAACTATGGAGA+AGG | 0.511952 | 5:-24689011 | MsG0580025821.01.T01:CDS |
| TAATATAGGTGGGAAGGACA+AGG | 0.515798 | 5:-24689365 | MsG0580025821.01.T01:intron |
| TAAGGGTATGCTGATGATGA+TGG | 0.520173 | 5:+24687950 | None:intergenic |
| GATCTTCTTACTTGGGTGGA+GGG | 0.521140 | 5:+24689576 | None:intergenic |
| TGTGTATAATATAGGTGGGA+AGG | 0.521340 | 5:-24689371 | MsG0580025821.01.T01:intron |
| AAAAGTTGCGAAAGAAGAGT+TGG | 0.521509 | 5:-24687672 | MsG0580025821.01.T01:CDS |
| AAGTTGTGCCATTGTTCTGA+TGG | 0.534050 | 5:+24687920 | None:intergenic |
| ACAATCTTCACACATTTGCA+TGG | 0.536085 | 5:+24687317 | None:intergenic |
| GGAAGAACATCTTACTCCAT+TGG | 0.536644 | 5:-24689699 | MsG0580025821.01.T01:CDS |
| CTTGATGGAAATGATGTTGA+TGG | 0.536878 | 5:+24687576 | None:intergenic |
| TCAGGTTGAAGACCTTGGTA+AGG | 0.540371 | 5:+24689056 | None:intergenic |
| TTGCATCCTTGAACAGCAGC+AGG | 0.542861 | 5:+24687204 | None:intergenic |
| CCATAAACTGATGATGAGCT+AGG | 0.545039 | 5:+24689098 | None:intergenic |
| GGGTGTGGAAGTAGCACTCC+TGG | 0.547961 | 5:+24687971 | None:intergenic |
| AAAGTTCAGGTTGAAGACCT+TGG | 0.548638 | 5:+24689051 | None:intergenic |
| GTGCTTATATAAAGGAGGAT+AGG | 0.553115 | 5:-24689884 | MsG0580025821.01.T01:five_prime_UTR |
| CATTTCCATCAAGATCATCA+TGG | 0.554525 | 5:-24687566 | MsG0580025821.01.T01:CDS |
| AAATTAATTTCTTGTCAGCA+TGG | 0.554884 | 5:-24688807 | MsG0580025821.01.T01:CDS |
| ACAACAGCAACAAAGAGCAT+TGG | 0.565036 | 5:-24687714 | MsG0580025821.01.T01:CDS |
| ACCTTCTCTAACAGCAATGA+GGG | 0.566133 | 5:+24688465 | None:intergenic |
| GAGAGTAGTAGTTCAATACC+AGG | 0.567679 | 5:-24687602 | MsG0580025821.01.T01:CDS |
| AGAAGATGAGTAACAGCCAA+TGG | 0.572438 | 5:+24689683 | None:intergenic |
| GTAGTGTATTGTAGTATGAA+GGG | 0.572719 | 5:-24687361 | MsG0580025821.01.T01:three_prime_UTR |
| AGGTATCTTTAATAATAGTA+GGG | 0.573912 | 5:+24689850 | None:intergenic |
| CTGCAGATCATAGTCACAAG+TGG | 0.577564 | 5:-24688855 | MsG0580025821.01.T01:CDS |
| ACAGCAGCAGGAGCTAGCAT+TGG | 0.582155 | 5:+24687216 | None:intergenic |
| TATGCTGATGATGATGGGTG+TGG | 0.582762 | 5:+24687956 | None:intergenic |
| AATATAGGTGGGAAGGACAA+GGG | 0.583406 | 5:-24689364 | MsG0580025821.01.T01:intron |
| ACTGAGCTTCCCAAGTCCTA+GGG | 0.584163 | 5:+24688633 | None:intergenic |
| CACCTTCTCTAACAGCAATG+AGG | 0.591099 | 5:+24688464 | None:intergenic |
| GCCCTCATTGCTGTTAGAGA+AGG | 0.593948 | 5:-24688466 | MsG0580025821.01.T01:CDS |
| ATATAGGTGGGAAGGACAAG+GGG | 0.594932 | 5:-24689363 | MsG0580025821.01.T01:intron |
| AGCATTGCACCACAGCATCA+AGG | 0.600171 | 5:-24687890 | MsG0580025821.01.T01:CDS |
| GGGGCATATGATAGATCAAG+AGG | 0.613030 | 5:-24689344 | MsG0580025821.01.T01:CDS |
| TGTTGTGGTGGAACAACTGA+AGG | 0.615956 | 5:+24687776 | None:intergenic |
| AGATCTATAACTATGGAGAA+GGG | 0.626888 | 5:-24689010 | MsG0580025821.01.T01:intron |
| TCAATTAGGAGCTGTTCACA+AGG | 0.628502 | 5:-24688437 | MsG0580025821.01.T01:intron |
| ATAGATCAAGAGGAAACAGA+AGG | 0.628702 | 5:-24689334 | MsG0580025821.01.T01:CDS |
| AAGGGTATGCTGATGATGAT+GGG | 0.632300 | 5:+24687951 | None:intergenic |
| AATTGATCACCTTGATGCTG+TGG | 0.645054 | 5:+24687881 | None:intergenic |
| AGACCTGAACAACTTGATGT+TGG | 0.656658 | 5:-24687626 | MsG0580025821.01.T01:CDS |
| GAATTAATTCAACCTTACCA+AGG | 0.657107 | 5:-24689068 | MsG0580025821.01.T01:CDS |
| ATGGTGGTCCATCAGAACAA+TGG | 0.666115 | 5:-24687928 | MsG0580025821.01.T01:CDS |
| GATTGAAGACTTGAGCTATG+TGG | 0.668398 | 5:-24688032 | MsG0580025821.01.T01:CDS |
| GGTGTGTGCTTATATAAAGG+AGG | 0.669304 | 5:-24689889 | None:intergenic |
| TCATCAGCATACCCTTATGG+TGG | 0.673613 | 5:-24687944 | MsG0580025821.01.T01:CDS |
| AAATGATGTTGATGGTACCC+TGG | 0.680740 | 5:+24687584 | None:intergenic |
| CATACTACAATACACTACCA+AGG | 0.692930 | 5:+24687366 | None:intergenic |
| GCAGCACCTGAAATAAACAC+TGG | 0.697600 | 5:-24689128 | MsG0580025821.01.T01:CDS |
| AGAGTAGTAGTTCAATACCA+GGG | 0.726230 | 5:-24687601 | MsG0580025821.01.T01:CDS |
| ATGTTGATCCAAGTACAATG+TGG | 0.823704 | 5:+24687239 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAATTAAATTAAAGAA+AGG | + | Chr5:24688553-24688572 | None:intergenic | 10.0% |
| !! | ACTTGATTATAAAAAAAAAA+TGG | - | Chr5:24687787-24687806 | MsG0580025821.01.T01:CDS | 10.0% |
| !!! | ATATTTTTTGTGTATAATAT+AGG | - | Chr5:24687685-24687704 | MsG0580025821.01.T01:CDS | 10.0% |
| !!! | TATTAGTTAATTAGATTTTA+TGG | - | Chr5:24688164-24688183 | MsG0580025821.01.T01:intron | 10.0% |
| !!! | TGAGAATTTTATTTTATATA+TGG | - | Chr5:24687344-24687363 | MsG0580025821.01.T01:three_prime_UTR | 10.0% |
| !! | AAATTAATTTCTTGATCATT+AGG | + | Chr5:24688247-24688266 | None:intergenic | 15.0% |
| !! | AATGGATCAAATAATAATAA+TGG | - | Chr5:24689522-24689541 | MsG0580025821.01.T01:intron | 15.0% |
| !! | AGGTATCTTTAATAATAGTA+GGG | + | Chr5:24687217-24687236 | None:intergenic | 20.0% |
| !! | CTGGTAATAATTTAAGAAAA+TGG | + | Chr5:24688811-24688830 | None:intergenic | 20.0% |
| !! | TAGGTATCTTTAATAATAGT+AGG | + | Chr5:24687218-24687237 | None:intergenic | 20.0% |
| !! | TAGTTGCATATATATATATG+AGG | - | Chr5:24687592-24687611 | MsG0580025821.01.T01:CDS | 20.0% |
| !!! | AAATAAAAGTATGTGAATGA+GGG | + | Chr5:24688511-24688530 | None:intergenic | 20.0% |
| !!! | GAAGTTTTAATTAAGCAAAT+AGG | - | Chr5:24688139-24688158 | MsG0580025821.01.T01:intron | 20.0% |
| !!! | TAAATAAAAGTATGTGAATG+AGG | + | Chr5:24688512-24688531 | None:intergenic | 20.0% |
| !!! | TTTTTGTGTATAATATAGGT+GGG | - | Chr5:24687689-24687708 | MsG0580025821.01.T01:CDS | 20.0% |
| !!! | TTTTTTGTGTATAATATAGG+TGG | - | Chr5:24687688-24687707 | MsG0580025821.01.T01:CDS | 20.0% |
| ! | AAATTAATTTCTTGTCAGCA+TGG | - | Chr5:24688257-24688276 | MsG0580025821.01.T01:intron | 25.0% |
| ! | GAAATCATTCTATCTATTTC+AGG | + | Chr5:24688401-24688420 | None:intergenic | 25.0% |
| ! | TAAAGATACCTATAGCTAAA+GGG | - | Chr5:24687226-24687245 | MsG0580025821.01.T01:three_prime_UTR | 25.0% |
| ! | TTAAAGATACCTATAGCTAA+AGG | - | Chr5:24687225-24687244 | MsG0580025821.01.T01:three_prime_UTR | 25.0% |
| ! | TTTGATTGAGTGAGTATAAT+TGG | + | Chr5:24688988-24689007 | None:intergenic | 25.0% |
| !! | AACACAAAATCAACATGTTT+TGG | - | Chr5:24689323-24689342 | MsG0580025821.01.T01:intron | 25.0% |
| !! | TCAATCAAATTTTGTCTCTA+TGG | - | Chr5:24688999-24689018 | MsG0580025821.01.T01:intron | 25.0% |
| !! | TTAATTAGATTTTATGGAGC+AGG | - | Chr5:24688170-24688189 | MsG0580025821.01.T01:intron | 25.0% |
| !! | TTTTGTCTCTATGGAATTTA+AGG | - | Chr5:24689008-24689027 | MsG0580025821.01.T01:intron | 25.0% |
| !!! | GTTTGATTTTGAATTTGTTG+TGG | + | Chr5:24689306-24689325 | None:intergenic | 25.0% |
| !!! | TCATGATTTTTTATGTCTCT+CGG | + | Chr5:24688890-24688909 | None:intergenic | 25.0% |
| AGATCTATAACTATGGAGAA+GGG | - | Chr5:24688054-24688073 | MsG0580025821.01.T01:intron | 30.0% | |
| CATCCATGAAAATTCTCAAT+GGG | - | Chr5:24687428-24687447 | MsG0580025821.01.T01:three_prime_UTR | 30.0% | |
| GAATTAATTCAACCTTACCA+AGG | - | Chr5:24687996-24688015 | MsG0580025821.01.T01:CDS | 30.0% | |
| GTAGTGTATTGTAGTATGAA+GGG | - | Chr5:24689703-24689722 | MsG0580025821.01.T01:CDS | 30.0% | |
| TCACATGAGATCTATAACTA+TGG | - | Chr5:24688047-24688066 | MsG0580025821.01.T01:intron | 30.0% | |
| TGCATATTTCAGTTTCTTCT+TGG | - | Chr5:24689674-24689693 | MsG0580025821.01.T01:CDS | 30.0% | |
| TGTTAGACACAAACATAAGT+CGG | + | Chr5:24688749-24688768 | None:intergenic | 30.0% | |
| TTGCTATGATCTTCTTACTT+GGG | + | Chr5:24687498-24687517 | None:intergenic | 30.0% | |
| ! | AAACCCATTGAGAATTTTCA+TGG | + | Chr5:24687434-24687453 | None:intergenic | 30.0% |
| ! | AACCATGTTTGAGATAACAA+AGG | + | Chr5:24688665-24688684 | None:intergenic | 30.0% |
| ! | ACCATGTTTGAGATAACAAA+GGG | + | Chr5:24688664-24688683 | None:intergenic | 30.0% |
| ! | ATTGATGGAGTTACTTTCAT+TGG | + | Chr5:24689237-24689256 | None:intergenic | 30.0% |
| ! | TTTCAGATTTAAGACGTGTT+TGG | - | Chr5:24688720-24688739 | MsG0580025821.01.T01:intron | 30.0% |
| ! | TTTGCTATGATCTTCTTACT+TGG | + | Chr5:24687499-24687518 | None:intergenic | 30.0% |
| !!! | GTGCAATGTTTTAAAGACAA+AGG | + | Chr5:24688939-24688958 | None:intergenic | 30.0% |
| !!! | TGATTTTGAATTTGTTGTGG+TGG | + | Chr5:24689303-24689322 | None:intergenic | 30.0% |
| AAAAGTTGCGAAAGAAGAGT+TGG | - | Chr5:24689392-24689411 | MsG0580025821.01.T01:intron | 35.0% | |
| ACAATCTTCACACATTTGCA+TGG | + | Chr5:24689750-24689769 | None:intergenic | 35.0% | |
| AGAGTAGTAGTTCAATACCA+GGG | - | Chr5:24689463-24689482 | MsG0580025821.01.T01:intron | 35.0% | |
| ATAGATCAAGAGGAAACAGA+AGG | - | Chr5:24687730-24687749 | MsG0580025821.01.T01:CDS | 35.0% | |
| ATGAAACACAACACTGATAC+TGG | + | Chr5:24688830-24688849 | None:intergenic | 35.0% | |
| ATGTTGATCCAAGTACAATG+TGG | + | Chr5:24689828-24689847 | None:intergenic | 35.0% | |
| ATTGTAGTGAGAGAGTTGTT+AGG | - | Chr5:24689614-24689633 | MsG0580025821.01.T01:CDS | 35.0% | |
| CAATCACCAGTGTTTATTTC+AGG | + | Chr5:24687945-24687964 | None:intergenic | 35.0% | |
| CACACATTTGCATGGAATAA+TGG | + | Chr5:24689742-24689761 | None:intergenic | 35.0% | |
| CATACTACAATACACTACCA+AGG | + | Chr5:24689701-24689720 | None:intergenic | 35.0% | |
| CATCAAGATCATCATGGAAA+TGG | - | Chr5:24689504-24689523 | MsG0580025821.01.T01:intron | 35.0% | |
| CATTTCCATCAAGATCATCA+TGG | - | Chr5:24689498-24689517 | MsG0580025821.01.T01:intron | 35.0% | |
| CATTTCCATGATGATCTTGA+TGG | + | Chr5:24689506-24689525 | None:intergenic | 35.0% | |
| CTAGCTCATCATCAGTTTAT+GGG | - | Chr5:24687967-24687986 | MsG0580025821.01.T01:CDS | 35.0% | |
| CTCTCTCACTACAATATGTT+AGG | + | Chr5:24689609-24689628 | None:intergenic | 35.0% | |
| CTTGATGGAAATGATGTTGA+TGG | + | Chr5:24689491-24689510 | None:intergenic | 35.0% | |
| GACATCTTGAAGAAAAGTTC+AGG | + | Chr5:24688029-24688048 | None:intergenic | 35.0% | |
| GAGATCTATAACTATGGAGA+AGG | - | Chr5:24688053-24688072 | MsG0580025821.01.T01:intron | 35.0% | |
| GCATCCATGAAAATTCTCAA+TGG | - | Chr5:24687427-24687446 | MsG0580025821.01.T01:three_prime_UTR | 35.0% | |
| GGTAGTGTATTGTAGTATGA+AGG | - | Chr5:24689702-24689721 | MsG0580025821.01.T01:CDS | 35.0% | |
| GTATCAGTGTTGTGTTTCAT+AGG | - | Chr5:24688830-24688849 | MsG0580025821.01.T01:CDS | 35.0% | |
| GTGCTTATATAAAGGAGGAT+AGG | - | Chr5:24687180-24687199 | MsG0580025821.01.T01:three_prime_UTR | 35.0% | |
| GTTGGATAATGAAGAAGAAG+AGG | - | Chr5:24689410-24689429 | MsG0580025821.01.T01:intron | 35.0% | |
| TATATGCATGCACAGGATAT+TGG | - | Chr5:24687878-24687897 | MsG0580025821.01.T01:CDS | 35.0% | |
| TCAGTTCTTGTCTGGTATAA+AGG | - | Chr5:24688449-24688468 | MsG0580025821.01.T01:CDS | 35.0% | |
| TCTATGTTATATGCATGCAC+AGG | - | Chr5:24687871-24687890 | MsG0580025821.01.T01:CDS | 35.0% | |
| TCTTGAAACCCTTTAGCTAT+AGG | + | Chr5:24687237-24687256 | None:intergenic | 35.0% | |
| TGTGTATAATATAGGTGGGA+AGG | - | Chr5:24687693-24687712 | MsG0580025821.01.T01:CDS | 35.0% | |
| ! | TCCCTTTGTTATCTCAAACA+TGG | - | Chr5:24688660-24688679 | MsG0580025821.01.T01:intron | 35.0% |
| !! | AGAGAAGGTGTTGTTCAATT+AGG | - | Chr5:24688613-24688632 | MsG0580025821.01.T01:intron | 35.0% |
| !! | CAAAGAGCATTGGAATTTAC+AGG | - | Chr5:24689360-24689379 | MsG0580025821.01.T01:CDS | 35.0% |
| !!! | AATGGGTTTATGCTGTTTTC+TGG | - | Chr5:24687445-24687464 | MsG0580025821.01.T01:three_prime_UTR | 35.0% |
| !!! | ATTTTATGGAGCAGGTTGAT+TGG | - | Chr5:24688178-24688197 | MsG0580025821.01.T01:intron | 35.0% |
| AATATAGGTGGGAAGGACAA+GGG | - | Chr5:24687700-24687719 | MsG0580025821.01.T01:CDS | 40.0% | |
| AATTGATCACCTTGATGCTG+TGG | + | Chr5:24689186-24689205 | None:intergenic | 40.0% | |
| ACCTTCTCTAACAGCAATGA+GGG | + | Chr5:24688602-24688621 | None:intergenic | 40.0% | |
| AGAAGATGAGTAACAGCCAA+TGG | + | Chr5:24687384-24687403 | None:intergenic | 40.0% | |
| AGACCTGAACAACTTGATGT+TGG | - | Chr5:24689438-24689457 | MsG0580025821.01.T01:intron | 40.0% | |
| CAAGAGGAAACAGAAGGAAT+TGG | - | Chr5:24687736-24687755 | MsG0580025821.01.T01:CDS | 40.0% | |
| CCAAGTACAATGTGGTAGAT+TGG | + | Chr5:24689820-24689839 | None:intergenic | 40.0% | |
| CCAATCTACCACATTGTACT+TGG | - | Chr5:24689817-24689836 | MsG0580025821.01.T01:five_prime_UTR | 40.0% | |
| CCTAGCTCATCATCAGTTTA+TGG | - | Chr5:24687966-24687985 | MsG0580025821.01.T01:CDS | 40.0% | |
| CTATGATCTTCTTACTTGGG+TGG | + | Chr5:24687495-24687514 | None:intergenic | 40.0% | |
| GAACAATGGCACAACTTTCA+AGG | - | Chr5:24689150-24689169 | MsG0580025821.01.T01:CDS | 40.0% | |
| GAGAGTAGTAGTTCAATACC+AGG | - | Chr5:24689462-24689481 | MsG0580025821.01.T01:intron | 40.0% | |
| GATTGAAGACTTGAGCTATG+TGG | - | Chr5:24689032-24689051 | MsG0580025821.01.T01:CDS | 40.0% | |
| GCTTCAAGACTACTCATTGA+TGG | + | Chr5:24689252-24689271 | None:intergenic | 40.0% | |
| GGAAGAACATCTTACTCCAT+TGG | - | Chr5:24687365-24687384 | MsG0580025821.01.T01:three_prime_UTR | 40.0% | |
| TAATATAGGTGGGAAGGACA+AGG | - | Chr5:24687699-24687718 | MsG0580025821.01.T01:CDS | 40.0% | |
| TCAATTAGGAGCTGTTCACA+AGG | - | Chr5:24688627-24688646 | MsG0580025821.01.T01:CDS | 40.0% | |
| TCATAGATCAGTCGCATTCT+CGG | - | Chr5:24688912-24688931 | MsG0580025821.01.T01:intron | 40.0% | |
| TCATCATCAGCATACCCTTA+TGG | - | Chr5:24689117-24689136 | MsG0580025821.01.T01:CDS | 40.0% | |
| TTCAGTTTCTTCTTGGACCT+TGG | - | Chr5:24689681-24689700 | MsG0580025821.01.T01:CDS | 40.0% | |
| ! | AAGGGTATGCTGATGATGAT+GGG | + | Chr5:24689116-24689135 | None:intergenic | 40.0% |
| ! | AAGTTGTGCCATTGTTCTGA+TGG | + | Chr5:24689147-24689166 | None:intergenic | 40.0% |
| ! | ACAACAGCAACAAAGAGCAT+TGG | - | Chr5:24689350-24689369 | MsG0580025821.01.T01:CDS | 40.0% |
| ! | AGCTACATTGAAAGCATACC+AGG | - | Chr5:24689075-24689094 | MsG0580025821.01.T01:CDS | 40.0% |
| ! | CCATAAACTGATGATGAGCT+AGG | + | Chr5:24687969-24687988 | None:intergenic | 40.0% |
| ! | GATTTCTTTTACAGCACCCT+AGG | - | Chr5:24688415-24688434 | MsG0580025821.01.T01:intron | 40.0% |
| ! | TAAGGGTATGCTGATGATGA+TGG | + | Chr5:24689117-24689136 | None:intergenic | 40.0% |
| ! | TCACCAACATCAAGTTGTTC+AGG | + | Chr5:24689444-24689463 | None:intergenic | 40.0% |
| !! | AAAGTTCAGGTTGAAGACCT+TGG | + | Chr5:24688016-24688035 | None:intergenic | 40.0% |
| !! | AAATGATGTTGATGGTACCC+TGG | + | Chr5:24689483-24689502 | None:intergenic | 40.0% |
| !! | AGGATATTGGTTTGGGAAGA+TGG | - | Chr5:24687891-24687910 | MsG0580025821.01.T01:CDS | 40.0% |
| !! | CATGCACAGGATATTGGTTT+GGG | - | Chr5:24687884-24687903 | MsG0580025821.01.T01:CDS | 40.0% |
| ATATAGGTGGGAAGGACAAG+GGG | - | Chr5:24687701-24687720 | MsG0580025821.01.T01:CDS | 45.0% | |
| ATGGTGGTCCATCAGAACAA+TGG | - | Chr5:24689136-24689155 | MsG0580025821.01.T01:CDS | 45.0% | |
| ATGTCTCTCGGATTGCAACA+TGG | + | Chr5:24688878-24688897 | None:intergenic | 45.0% | |
| CACCTTCTCTAACAGCAATG+AGG | + | Chr5:24688603-24688622 | None:intergenic | 45.0% | |
| CTGCAGATCATAGTCACAAG+TGG | - | Chr5:24688209-24688228 | MsG0580025821.01.T01:intron | 45.0% | |
| GATCTTCTTACTTGGGTGGA+GGG | + | Chr5:24687491-24687510 | None:intergenic | 45.0% | |
| GCAGCACCTGAAATAAACAC+TGG | - | Chr5:24687936-24687955 | MsG0580025821.01.T01:CDS | 45.0% | |
| GGGGCATATGATAGATCAAG+AGG | - | Chr5:24687720-24687739 | MsG0580025821.01.T01:CDS | 45.0% | |
| TCATCAGCATACCCTTATGG+TGG | - | Chr5:24689120-24689139 | MsG0580025821.01.T01:CDS | 45.0% | |
| TGATCTTCTTACTTGGGTGG+AGG | + | Chr5:24687492-24687511 | None:intergenic | 45.0% | |
| TTTACAGCACCCTAGGACTT+GGG | - | Chr5:24688422-24688441 | MsG0580025821.01.T01:intron | 45.0% | |
| ! | GCATGCACAGGATATTGGTT+TGG | - | Chr5:24687883-24687902 | MsG0580025821.01.T01:CDS | 45.0% |
| ! | GTTCTGATGGACCACCATAA+GGG | + | Chr5:24689134-24689153 | None:intergenic | 45.0% |
| ! | TGTTCTGATGGACCACCATA+AGG | + | Chr5:24689135-24689154 | None:intergenic | 45.0% |
| ! | TGTTGTGGTGGAACAACTGA+AGG | + | Chr5:24689291-24689310 | None:intergenic | 45.0% |
| ! | TTTTACAGCACCCTAGGACT+TGG | - | Chr5:24688421-24688440 | MsG0580025821.01.T01:intron | 45.0% |
| !! | TATGCTGATGATGATGGGTG+TGG | + | Chr5:24689111-24689130 | None:intergenic | 45.0% |
| !! | TCAGGTTGAAGACCTTGGTA+AGG | + | Chr5:24688011-24688030 | None:intergenic | 45.0% |
| !!! | GGGTTTATGCTGTTTTCTGG+AGG | - | Chr5:24687448-24687467 | MsG0580025821.01.T01:three_prime_UTR | 45.0% |
| AACTGAGCTTCCCAAGTCCT+AGG | + | Chr5:24688435-24688454 | None:intergenic | 50.0% | |
| ACTGAGCTTCCCAAGTCCTA+GGG | + | Chr5:24688434-24688453 | None:intergenic | 50.0% | |
| AGCATTGCACCACAGCATCA+AGG | - | Chr5:24689174-24689193 | MsG0580025821.01.T01:CDS | 50.0% | |
| CTCGGATTGCAACATGGTTG+CGG | + | Chr5:24688872-24688891 | None:intergenic | 50.0% | |
| TGGGAAGCTCAGTTCTTGTC+TGG | - | Chr5:24688441-24688460 | MsG0580025821.01.T01:CDS | 50.0% | |
| TTGCATCCTTGAACAGCAGC+AGG | + | Chr5:24689863-24689882 | None:intergenic | 50.0% | |
| TTGGGTGGAGGGTAGTTTCT+AGG | + | Chr5:24687480-24687499 | None:intergenic | 50.0% | |
| ! | GCCCTCATTGCTGTTAGAGA+AGG | - | Chr5:24688598-24688617 | MsG0580025821.01.T01:intron | 50.0% |
| ACAGCAGCAGGAGCTAGCAT+TGG | + | Chr5:24689851-24689870 | None:intergenic | 55.0% | |
| CTAGCTCCTGCTGCTGTTCA+AGG | - | Chr5:24689854-24689873 | MsG0580025821.01.T01:five_prime_UTR | 55.0% | |
| GGGTGTGGAAGTAGCACTCC+TGG | + | Chr5:24689096-24689115 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 24687180 | 24689906 | 24687180 | ID=MsG0580025821.01;Name=MsG0580025821.01 |
| Chr5 | mRNA | 24687180 | 24689906 | 24687180 | ID=MsG0580025821.01.T01;Parent=MsG0580025821.01;Name=MsG0580025821.01.T01;_AED=0.21;_eAED=0.21;_QI=183|1|1|1|1|1|7|355|365 |
| Chr5 | exon | 24687180 | 24688056 | 24687180 | ID=MsG0580025821.01.T01:exon:3994;Parent=MsG0580025821.01.T01 |
| Chr5 | exon | 24688438 | 24688494 | 24688438 | ID=MsG0580025821.01.T01:exon:3993;Parent=MsG0580025821.01.T01 |
| Chr5 | exon | 24688616 | 24688657 | 24688616 | ID=MsG0580025821.01.T01:exon:3992;Parent=MsG0580025821.01.T01 |
| Chr5 | exon | 24688789 | 24688894 | 24688789 | ID=MsG0580025821.01.T01:exon:3991;Parent=MsG0580025821.01.T01 |
| Chr5 | exon | 24689011 | 24689193 | 24689011 | ID=MsG0580025821.01.T01:exon:3990;Parent=MsG0580025821.01.T01 |
| Chr5 | exon | 24689329 | 24689379 | 24689329 | ID=MsG0580025821.01.T01:exon:3989;Parent=MsG0580025821.01.T01 |
| Chr5 | exon | 24689587 | 24689906 | 24689587 | ID=MsG0580025821.01.T01:exon:3988;Parent=MsG0580025821.01.T01 |
| Chr5 | five_prime_UTR | 24689724 | 24689906 | 24689724 | ID=MsG0580025821.01.T01:five_prime_utr;Parent=MsG0580025821.01.T01 |
| Chr5 | CDS | 24689587 | 24689723 | 24689587 | ID=MsG0580025821.01.T01:cds;Parent=MsG0580025821.01.T01 |
| Chr5 | CDS | 24689329 | 24689379 | 24689329 | ID=MsG0580025821.01.T01:cds;Parent=MsG0580025821.01.T01 |
| Chr5 | CDS | 24689011 | 24689193 | 24689011 | ID=MsG0580025821.01.T01:cds;Parent=MsG0580025821.01.T01 |
| Chr5 | CDS | 24688789 | 24688894 | 24688789 | ID=MsG0580025821.01.T01:cds;Parent=MsG0580025821.01.T01 |
| Chr5 | CDS | 24688616 | 24688657 | 24688616 | ID=MsG0580025821.01.T01:cds;Parent=MsG0580025821.01.T01 |
| Chr5 | CDS | 24688438 | 24688494 | 24688438 | ID=MsG0580025821.01.T01:cds;Parent=MsG0580025821.01.T01 |
| Chr5 | CDS | 24687535 | 24688056 | 24687535 | ID=MsG0580025821.01.T01:cds;Parent=MsG0580025821.01.T01 |
| Chr5 | three_prime_UTR | 24687180 | 24687534 | 24687180 | ID=MsG0580025821.01.T01:three_prime_utr;Parent=MsG0580025821.01.T01 |
| Gene Sequence |
| Protein sequence |
