Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038488.01.T01 | KEH22665.1 | 78.571 | 56 | 12 | 0 | 4 | 59 | 16 | 71 | 1.36E-24 | 100 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038488.01.T01 | A0A072U9Y5 | 78.571 | 56 | 12 | 0 | 4 | 59 | 16 | 71 | 6.51e-25 | 100 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047944.01 | MsG0780038488.01 | 0.803019 | 4.256533e-49 | 2.116017e-46 |
| MsG0080048404.01 | MsG0780038488.01 | 0.804649 | 1.952171e-49 | 1.012042e-46 |
| MsG0080048461.01 | MsG0780038488.01 | 0.822708 | 2.050288e-53 | 1.729341e-50 |
| MsG0080048462.01 | MsG0780038488.01 | 0.832051 | 1.178491e-55 | 1.302318e-52 |
| MsG0080048463.01 | MsG0780038488.01 | 0.813808 | 2.122038e-51 | 1.399793e-48 |
| MsG0080048464.01 | MsG0780038488.01 | 0.809239 | 2.087419e-50 | 1.219169e-47 |
| MsG0180000470.01 | MsG0780038488.01 | 0.827008 | 1.983568e-54 | 1.890932e-51 |
| MsG0180000914.01 | MsG0780038488.01 | 0.801938 | 7.108243e-49 | 3.437869e-46 |
| MsG0180002377.01 | MsG0780038488.01 | 0.825019 | 5.888452e-54 | 5.303988e-51 |
| MsG0180003455.01 | MsG0780038488.01 | 0.800475 | 1.416304e-48 | 6.600744e-46 |
| MsG0180003885.01 | MsG0780038488.01 | 0.813292 | 2.756142e-51 | 1.792705e-48 |
| MsG0180004249.01 | MsG0780038488.01 | 0.802299 | 5.992815e-49 | 2.925326e-46 |
| MsG0180004389.01 | MsG0780038488.01 | 0.813894 | 2.031673e-51 | 1.343277e-48 |
| MsG0180004500.01 | MsG0780038488.01 | 0.820544 | 6.484387e-53 | 5.145886e-50 |
| MsG0280006309.01 | MsG0780038488.01 | 0.810327 | 1.217800e-50 | 7.319197e-48 |
| MsG0280010722.01 | MsG0780038488.01 | 0.811756 | 5.971527e-51 | 3.727568e-48 |
| MsG0380011609.01 | MsG0780038488.01 | 0.818002 | 2.460024e-52 | 1.819443e-49 |
| MsG0380014537.01 | MsG0780038488.01 | 0.841598 | 4.311349e-58 | 6.367031e-55 |
| MsG0680031108.01 | MsG0780038488.01 | 0.801252 | 9.827156e-49 | 4.671218e-46 |
| MsG0680034230.01 | MsG0780038488.01 | 0.807704 | 4.438830e-50 | 2.489485e-47 |
| MsG0680035034.01 | MsG0780038488.01 | 0.804974 | 1.669781e-49 | 8.729561e-47 |
| MsG0680035580.01 | MsG0780038488.01 | 0.805962 | 1.036244e-49 | 5.556726e-47 |
| MsG0480018465.01 | MsG0780038488.01 | 0.804553 | 2.043962e-49 | 1.056986e-46 |
| MsG0480018653.01 | MsG0780038488.01 | 0.827860 | 1.238573e-54 | 1.210579e-51 |
| MsG0480018985.01 | MsG0780038488.01 | 0.808388 | 3.174086e-50 | 1.812572e-47 |
| MsG0480019711.01 | MsG0780038488.01 | 0.815559 | 8.688562e-52 | 6.010287e-49 |
| MsG0480020739.01 | MsG0780038488.01 | 0.801847 | 7.423839e-49 | 3.582287e-46 |
| MsG0480021870.01 | MsG0780038488.01 | 0.824323 | 8.588900e-54 | 7.582301e-51 |
| MsG0480022068.01 | MsG0780038488.01 | 0.823111 | 1.651099e-53 | 1.408672e-50 |
| MsG0480022514.01 | MsG0780038488.01 | 0.825214 | 5.294471e-54 | 4.795458e-51 |
| MsG0480022589.01 | MsG0780038488.01 | 0.804451 | 2.146532e-49 | 1.107109e-46 |
| MsG0480022985.01 | MsG0780038488.01 | 0.802832 | 4.651954e-49 | 2.301941e-46 |
| MsG0480023172.01 | MsG0780038488.01 | 0.814884 | 1.227163e-51 | 8.333490e-49 |
| MsG0480023251.01 | MsG0780038488.01 | 0.828891 | 6.987183e-55 | 7.036320e-52 |
| MsG0480023381.01 | MsG0780038488.01 | 0.802395 | 5.726394e-49 | 2.802079e-46 |
| MsG0480023488.01 | MsG0780038488.01 | 0.806326 | 8.685015e-50 | 4.700582e-47 |
| MsG0480023489.01 | MsG0780038488.01 | 0.804045 | 2.607882e-49 | 1.331158e-46 |
| MsG0480023670.01 | MsG0780038488.01 | 0.808598 | 2.862633e-50 | 1.643614e-47 |
| MsG0580025332.01 | MsG0780038488.01 | 0.806117 | 9.609724e-50 | 5.173528e-47 |
| MsG0580025661.01 | MsG0780038488.01 | 0.821672 | 3.565336e-53 | 2.919744e-50 |
| MsG0580025723.01 | MsG0780038488.01 | 0.814741 | 1.320457e-51 | 8.932739e-49 |
| MsG0580025816.01 | MsG0780038488.01 | 0.806326 | 8.686725e-50 | 4.701407e-47 |
| MsG0580025987.01 | MsG0780038488.01 | 0.844957 | 5.478645e-59 | 8.991050e-56 |
| MsG0380014948.01 | MsG0780038488.01 | 0.824558 | 7.564821e-54 | 6.724074e-51 |
| MsG0380015555.01 | MsG0780038488.01 | 0.839438 | 1.584133e-57 | 2.187759e-54 |
| MsG0380016285.01 | MsG0780038488.01 | 0.809873 | 1.525571e-50 | 9.060939e-48 |
| MsG0380016407.01 | MsG0780038488.01 | 0.806875 | 6.650496e-50 | 3.650490e-47 |
| MsG0380017293.01 | MsG0780038488.01 | 0.813050 | 3.113601e-51 | 2.011947e-48 |
| MsG0480018236.01 | MsG0780038488.01 | 0.817709 | 2.865072e-52 | 2.102065e-49 |
| MsG0580027211.01 | MsG0780038488.01 | 0.800231 | 1.587825e-48 | 7.354659e-46 |
| MsG0580027320.01 | MsG0780038488.01 | 0.804137 | 2.495565e-49 | 1.276817e-46 |
| MsG0580028517.01 | MsG0780038488.01 | 0.803196 | 3.911502e-49 | 1.953484e-46 |
| MsG0580029236.01 | MsG0780038488.01 | 0.848054 | 7.824186e-60 | 1.418021e-56 |
| MsG0280006770.01 | MsG0780038488.01 | 0.804126 | 2.508473e-49 | 1.283060e-46 |
| MsG0280006930.01 | MsG0780038488.01 | 0.835081 | 2.064807e-56 | 2.497842e-53 |
| MsG0280007955.01 | MsG0780038488.01 | 0.835302 | 1.816308e-56 | 2.212325e-53 |
| MsG0280008112.01 | MsG0780038488.01 | 0.839580 | 1.455199e-57 | 2.018272e-54 |
| MsG0280008113.01 | MsG0780038488.01 | 0.814843 | 1.253229e-51 | 8.501304e-49 |
| MsG0280008377.01 | MsG0780038488.01 | 0.814180 | 1.756559e-51 | 1.170424e-48 |
| MsG0280008738.01 | MsG0780038488.01 | 0.801889 | 7.276266e-49 | 3.514992e-46 |
| MsG0280008866.01 | MsG0780038488.01 | 0.810486 | 1.125544e-50 | 6.793217e-48 |
| MsG0280009421.01 | MsG0780038488.01 | 0.808767 | 2.634439e-50 | 1.519245e-47 |
| MsG0280010184.01 | MsG0780038488.01 | 0.813773 | 2.159738e-51 | 1.423241e-48 |
| MsG0280010349.01 | MsG0780038488.01 | 0.817801 | 2.731679e-52 | 2.009277e-49 |
| MsG0280010350.01 | MsG0780038488.01 | 0.827185 | 1.799019e-54 | 1.724048e-51 |
| MsG0280010366.01 | MsG0780038488.01 | 0.840701 | 7.421140e-58 | 1.065749e-54 |
| MsG0280010517.01 | MsG0780038488.01 | 0.803874 | 2.829789e-49 | 1.438144e-46 |
| MsG0780036602.01 | MsG0780038488.01 | 0.849599 | 2.915138e-60 | 5.555095e-57 |
| MsG0780037054.01 | MsG0780038488.01 | 0.816727 | 4.764353e-52 | 3.402647e-49 |
| MsG0780037141.01 | MsG0780038488.01 | 0.808000 | 3.839010e-50 | 2.169920e-47 |
| MsG0780037292.01 | MsG0780038488.01 | 0.804092 | 2.550390e-49 | 1.303364e-46 |
| MsG0780038488.01 | MsG0780039170.01 | 0.820856 | 5.498492e-53 | 4.401728e-50 |
| MsG0780038488.01 | MsG0780039443.01 | 0.835783 | 1.372567e-56 | 1.695846e-53 |
| MsG0780038488.01 | MsG0780040834.01 | 0.809285 | 2.040308e-50 | 1.193078e-47 |
| MsG0780038488.01 | MsG0780041187.01 | 0.818703 | 1.706821e-52 | 1.286945e-49 |
| MsG0780038488.01 | MsG0780041416.01 | 0.806965 | 6.365513e-50 | 3.502143e-47 |
| MsG0780038488.01 | MsG0880042520.01 | 0.832224 | 1.067660e-55 | 1.185753e-52 |
| MsG0780038488.01 | MsG0880043157.01 | 0.820463 | 6.770394e-53 | 5.360935e-50 |
| MsG0780038488.01 | MsG0880043941.01 | 0.810390 | 1.180359e-50 | 7.105788e-48 |
| MsG0780038488.01 | MsG0880044131.01 | 0.804241 | 2.374188e-49 | 1.217889e-46 |
| MsG0780038488.01 | MsG0880045111.01 | 0.818457 | 1.940566e-52 | 1.453492e-49 |
| MsG0780038488.01 | MsG0880045639.01 | 0.808763 | 2.639839e-50 | 1.522216e-47 |
| MsG0780038488.01 | MsG0880046815.01 | 0.808109 | 3.639542e-50 | 2.063023e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038488.01.T01 | MTR_7g056370 | 78.571 | 56 | 12 | 0 | 4 | 59 | 16 | 71 | 1.65e-28 | 100 |
| MsG0780038488.01.T01 | MTR_8g006930 | 58.333 | 60 | 24 | 1 | 1 | 59 | 33 | 92 | 4.12e-18 | 75.9 |
| MsG0780038488.01.T01 | MTR_7g056393 | 60.000 | 60 | 23 | 1 | 1 | 59 | 33 | 92 | 2.52e-17 | 73.6 |
| MsG0780038488.01.T01 | MTR_4g119410 | 58.491 | 53 | 22 | 0 | 7 | 59 | 40 | 92 | 1.39e-16 | 71.6 |
| MsG0780038488.01.T01 | MTR_7g078010 | 56.604 | 53 | 23 | 0 | 7 | 59 | 40 | 92 | 1.55e-15 | 68.2 |
| MsG0780038488.01.T01 | MTR_7g078010 | 56.604 | 53 | 23 | 0 | 7 | 59 | 40 | 92 | 2.26e-15 | 68.2 |
| MsG0780038488.01.T01 | MTR_8g063070 | 49.057 | 53 | 27 | 0 | 7 | 59 | 40 | 92 | 2.01e-14 | 65.5 |
| MsG0780038488.01.T01 | MTR_4g024660 | 54.717 | 53 | 24 | 0 | 7 | 59 | 40 | 92 | 4.80e-14 | 64.3 |
| MsG0780038488.01.T01 | MTR_8g469400 | 54.000 | 50 | 23 | 0 | 7 | 56 | 40 | 89 | 2.92e-13 | 62.4 |
| MsG0780038488.01.T01 | MTR_8g062600 | 50.943 | 53 | 26 | 0 | 7 | 59 | 40 | 92 | 4.03e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAGGAATGAAGGGTTTA+CGG | 0.345850 | 7:-47609437 | None:intergenic |
| CATGTCAGGCCCTTTGAATC+TGG | 0.373499 | 7:+47609268 | None:intergenic |
| GGAATGAAGGGTTTACGGTT+TGG | 0.382805 | 7:-47609432 | MsG0780038488.01.T01:CDS |
| TTCACCGATGTCCAACTTGC+CGG | 0.421229 | 7:+47609380 | None:intergenic |
| TTTACGGTTTGGAATAACTA+AGG | 0.451924 | 7:-47609421 | MsG0780038488.01.T01:CDS |
| ACTCGGCGTGTGGAAGTAAT+CGG | 0.488706 | 7:-47609291 | MsG0780038488.01.T01:CDS |
| AAGTAATCGGCCAGATTCAA+AGG | 0.528283 | 7:-47609278 | MsG0780038488.01.T01:CDS |
| GTTCCTAGTATCTTCTACAC+CGG | 0.539134 | 7:-47609399 | MsG0780038488.01.T01:CDS |
| TTGCCGGTGTAGAAGATACT+AGG | 0.545166 | 7:+47609396 | None:intergenic |
| ATGTCTTTGAAGTCTACTGT+AGG | 0.556818 | 7:+47609327 | None:intergenic |
| CACTAATCCAACTCGGCGTG+TGG | 0.564601 | 7:-47609301 | MsG0780038488.01.T01:CDS |
| TGTCTTTGAAGTCTACTGTA+GGG | 0.566281 | 7:+47609328 | None:intergenic |
| TCGAAGGTTTCAGGAATGAA+GGG | 0.576563 | 7:-47609444 | None:intergenic |
| AGTAATCGGCCAGATTCAAA+GGG | 0.579528 | 7:-47609277 | MsG0780038488.01.T01:CDS |
| TATCTTCTACACCGGCAAGT+TGG | 0.579874 | 7:-47609391 | MsG0780038488.01.T01:CDS |
| ATTACTTCCACACGCCGAGT+TGG | 0.581539 | 7:+47609294 | None:intergenic |
| ATCGGTGAAAACTCGTCTAG+TGG | 0.606720 | 7:-47609366 | MsG0780038488.01.T01:CDS |
| TACACCGGCAAGTTGGACAT+CGG | 0.663383 | 7:-47609384 | MsG0780038488.01.T01:CDS |
| ACATTCACACTAATCCAACT+CGG | 0.689636 | 7:-47609308 | MsG0780038488.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTACGGTTTGGAATAACTA+AGG | - | Chr7:47609280-47609299 | MsG0780038488.01.T01:CDS | 30.0% |
| ACATTCACACTAATCCAACT+CGG | - | Chr7:47609393-47609412 | MsG0780038488.01.T01:CDS | 35.0% | |
| !! | ATGTCTTTGAAGTCTACTGT+AGG | + | Chr7:47609377-47609396 | None:intergenic | 35.0% |
| !! | TGTCTTTGAAGTCTACTGTA+GGG | + | Chr7:47609376-47609395 | None:intergenic | 35.0% |
| AAGTAATCGGCCAGATTCAA+AGG | - | Chr7:47609423-47609442 | MsG0780038488.01.T01:CDS | 40.0% | |
| AGTAATCGGCCAGATTCAAA+GGG | - | Chr7:47609424-47609443 | MsG0780038488.01.T01:CDS | 40.0% | |
| GTTCCTAGTATCTTCTACAC+CGG | - | Chr7:47609302-47609321 | MsG0780038488.01.T01:CDS | 40.0% | |
| ATCGGTGAAAACTCGTCTAG+TGG | - | Chr7:47609335-47609354 | MsG0780038488.01.T01:CDS | 45.0% | |
| TATCTTCTACACCGGCAAGT+TGG | - | Chr7:47609310-47609329 | MsG0780038488.01.T01:CDS | 45.0% | |
| TTGCCGGTGTAGAAGATACT+AGG | + | Chr7:47609308-47609327 | None:intergenic | 45.0% | |
| ACTCGGCGTGTGGAAGTAAT+CGG | - | Chr7:47609410-47609429 | MsG0780038488.01.T01:CDS | 50.0% | |
| ATTACTTCCACACGCCGAGT+TGG | + | Chr7:47609410-47609429 | None:intergenic | 50.0% | |
| TTCACCGATGTCCAACTTGC+CGG | + | Chr7:47609324-47609343 | None:intergenic | 50.0% | |
| ! | TACACCGGCAAGTTGGACAT+CGG | - | Chr7:47609317-47609336 | MsG0780038488.01.T01:CDS | 50.0% |
| CACTAATCCAACTCGGCGTG+TGG | - | Chr7:47609400-47609419 | MsG0780038488.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 47609272 | 47609451 | 47609272 | ID=MsG0780038488.01;Name=MsG0780038488.01 |
| Chr7 | mRNA | 47609272 | 47609451 | 47609272 | ID=MsG0780038488.01.T01;Parent=MsG0780038488.01;Name=MsG0780038488.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|0|1|0|59 |
| Chr7 | exon | 47609272 | 47609451 | 47609272 | ID=MsG0780038488.01.T01:exon:19241;Parent=MsG0780038488.01.T01 |
| Chr7 | CDS | 47609272 | 47609451 | 47609272 | ID=MsG0780038488.01.T01:cds;Parent=MsG0780038488.01.T01 |
| Gene Sequence |
| Protein sequence |
